Project Summary Project Title: An Analysis ofFish Attraction and OxygenSanctuaryCreationUtilizing Newly DevelopedOxygenationTechniques Student: University of Arkansas Mentor: Engineering,University of Arkansas Departmentof Biological and Agricultural Abstract A significant causeof unproductivefish populationsin waterbodiesis low dissolvedoxygen(DO). Severalfactorscauselow DO at the bottomof an enclosedwater bodyincluding lack of photo-oxygenationdue to limited light andreducedreaerationdue to a decreaseor an obstructionof watercirculation. Low DO in bottom watercauses manydetrimentaleffectsin aquaticorganismsincluding decreasedreproduction, increasedsusceptibilityto disease,andmortality. This work will utilize a developmentaloxygendelivery devicecapableof delivering water supersaturated with oxygendirectly to the bottomof a waterbody. Prior researchhas shownthe DO deliveredby the device completelyremainsin solutionandis only lost through aerobicbiological processes.The devicewill be deployedto a regional lake,and its capabilitiesto producea sanctuaryof high DO water in lower levels of the water column will be tested. Fish response to the oxygen plume will be examined, and the potential for using this device for fish attractionand oxygensanctuaryproductionwill be detennined. An Analysis ofFish Attraction and Oxygen Sanctuary Creation Utilizing Newly Developed Oxygenation Techniques UndergraduateResearcher Mentor: Back2round Similar to terrestrial animals, fish and other aquatic organisms require oxygen to performmetabolic activities. Aquatic organismsobtain oxygenfrom microscopic oxygengasbubbles,or dissolvedoxygen(DO) in water, which passivelydiffusesinto an aquaticorganism'sbloodstream. The rate of oxygendiffusion dependson the concentrationof dissolvedoxygenin the water. If too Iowa DO concentrationexists, fish and otheraquaticlife can experiencedecreasedreproduction,hamperedswimming ability, increased susceptibility to disease,and mortality (Ricklefs and Miller, 2000). entersa water body through contactwith the atmospherein a processknown as reaeration (Chambersand Mill, 1996). During reaeration,atmosphericoxygenpassivelydiffuses into wateracrossthe surfaceboundaryof a waterbody becauseof the oxygen concentrationgradientexisting betweenair andwater. Oxygenalso entersa waterbody through plant photosynthesis according to the reaction 6CO2 + 6H2O + light -C~1206 is consumedfrom the waterduring aerobicrespirationby aquaticlife including bacteria, algae(during dark respiration),and fish. In a body of waterthe lowest concentrationof dissolvedoxygenis typically found at the lower levels of the watercolumn. In deepbodiesof water, light cannotreach bottom waterlevelspreventingphoto-oxygenation(phang,1991). During summer months,temperaturestratificationcanoccur,preventingcirculationbetweencold bottom water and warm surface water, and thereby not allowing bottom water accessto the atmosphere.Also, the majority of oxygenconsumptionoccursat the bottom where 1 2. sunkenorganicmater accumulatesand decomposesthroughaerobicprocesses(Deasand Orlob, 1999). The differencebetweenDO concentrationsat the surfaceand the bottom of a water body can be significant. During times of greattemperaturestratification or whenice coversthe surfaceandpreventsreaeration,the bottom watersof lakescan becomeanoxic. All aquaticorganismsrequire a specific level of DO. For a lake to be productive for a specific speciesof fish, the DO concentrationmustbe adequateat a water level wherethe water temperatureis desirablefor that species.Sincecold water fish species require low temperaturewaterto survive, they typically congregateat the bottom of a water body during warm weathermonths,making manylakesunproductiveor deadlyto the fish if DO levels at the bottom drop too low (USEPA, 1973). Introducing large quantitiesof oxygento the bottom of a waterbody mayprovide oxygenatedsanctuaries that could potentially attract fish and allow them to survive and even thrive during low DO periods. Objectives The objectivesof this project areto: Investigatethe feasibility of providing oxygensanctuariesat the bottom of a representativelake using a newly developedoxygenationtechnique. Deterntinethe effectsof the oxygenplume on attractingand sustaining populationsof fish. Methods Injecting bottom water with DO will be doneusingthe Portable WaterEcosystem O.xygenatordesigned by Osborn, Matlock, and Teltschikl. This device has been proven 1The Portable WaterEcosystemOxygenatoris patentpending. For the sake of brevity, full operatingdetails will not be discussedin this document. using research, Rogers, effective to deliver oxygen to water bodies at a rate 12 times faster than bubble aeration 1/6 of the energy(Osborn,2004). A National ScienceFoundationSBIR granthas beenreceivedfor further developingthis devicefor wastewatertreatment,and prior researchhasproven the devicecapableof treating remotelylocatedwaterbodies. The oxygenatorsupersaturates water with oxygenby atomizinghigh pressurewater within a vesselcontaininghigh pressureoxygengas. The deviceis then ableto deliver water supersaturated with dissolvedoxygento any locationin a water body with low DO (Osborn,2004). Sincethe devicecan inject oxygena greatdistanceaway from the physical location of the unit in the form of micro-bubblesthat rise much slowerthrough the watercolumn, the oxygenatorallows treatmentof bottom lake waterswithout disturbingthe surface. Field testscompletedduring researchthis summerwith an Honors College UndergraduateResearchGranthaveproventhe deviceis capableof delivering a precise mass of oxygen to a water body with virtually no DO loss to the atmosphere. The conductedon local streams,hasalso shownthe deviceis capableof creating large plumes of water saturated with oxygen that diminish only as biological processes consume the DO. During the research it was observed, but not quantified, that fish migratedupstreamto the sourceof oxygen.The deviceis the first aerationtool with the potential to raise and sustain the DO of water at the bottom of a body of water. Funding through a SURF grant will allow an undergraduateto conduct the first field trials with the Portable WaterEcosystemOxygenatorfor this application. The Portable Water EcosystemOxygenator will be deployed on Lake Atalanta in Arkansas. A cooperativeagreementhasbeenestablishedwith the City of Rogers Departmentof Parksand Recreationto pursuethis research.Officials haveagreedto provide initial support for this proj ect. Lake Atalanta features several optimal criteria for detennining fish responseto bottom water oxygenationincluding: a wide variety of fish of both cold water species -trout -and wann water species -bass, bream, catfish; an observedinability to supporttrout during summermonths likely due to low DO bottom water; and observedthermal stratificationpotentially creatingrelative high and low DO regions due to cold spring water inflow and atmospheric temperature variations. Prior to deployment,a depth,temperature,and oxygenconcentrationprofile will quantified for Lake Atalanta using a boat, GPSunit, andDO probe. Basedon the profile, two siteswill be selectedto oxygenate.One site will representa regionhaving a relatively saturated DO -likely a site near the inflow of the spring, and one site will represent a region of relatively low DO -likely a site near the bottom in the middle of the lake. The devicewill thenbe transportedand setup at the site. The oxygenatorwill be operatedfor a period of time at the lake while adjustingits working setpoints to achieve optimal oxygenation. During this preliminary test, observations will be made regarding the oxygenplume including its volume, DO, andmovement. Parametersincluding DO, temperature,depth, and positionwill be takenbefore,during, and afterrunning the device so to quantify to extent and duration of the oxygen plume. Oncethe datahas beenanalyzedand the behaviorof the oxygenplume is more clearlyunderstood,the devicewill be deployedat eachof the predetenninedsites. The magnitudeof fish concentrationin the oxygenplume will be measuredusing a wide area sonardevice. The numberof strikesreportedby the sonardevicewill representthe numberof fish in a given area. Imagesof the sonarscreenwill be takenfor further analysis. Sonar fish tallies, aswell asDO, temperature,depth,andposition, will be made one daybefore,two hoursbefore,during, two hours after, and one day after each deployment. Fish numberswill be takendirectly in and aroundthe entirevolume of the oxygenplume. Sonarmeasurements will alsobe takenbeforeand afterthe deployment Following in areasoutsidethe reachof the oxygenplume to give any indicationof fish movementto the plume. all deployments,datawill be analyzedto indicate anychangein fish concentrationas the deviceis deployed. Data regardingthe oxygenplume characteristics will also be further analyzed. A literature review will then be conducted to investigate the potential of the deviceto createoxygensanctuariesandto attractfish. An economic study will also be preforn1edto weigh the cost benefit ratio of using the device for such tasks. Si2nificance of Resear£h The Portable WaterEcosystemOxygenatoris capableof more effectively oxygenatingwater systemsthan traditional aerationprocesses.The techniqueemployed by this deviceis unique in that it can maintainan oxygenrich environmentat the bottom of the water column. The devicecould be usedto preventfish mortality and associated water quality problems,increasethe productivity of fish for aquicultureandrecreational activities, and assist in fish population studies. In addition to the data collected, the researchventurewill be an extremelyvaluablelearningexperience.This researchwill not only allow me to put the knowledgeobtainedin classinto real practice,but will accelerate my educational experiences and propel me to strive toward future research opportunities. The work will allow me to combine my personal interests in fishing and outdoor activities with engineering design research to create technological solutions to environmentalproblems. Project Schedule Jan2005 throughApril: This time period will be usedto preparefor all deployments.Trips will be madeto Lake Atalantaand officials from the city of Rogerswill be contactedto determinewater conditions,accesspoints to the lake, and electricity delivery. Delivery ratesrequiredof the oxygenatorwill be assessed and operatingadjustmentswill be madeto makethe deviceready for the specific deploymentsites. All preparatorymeasuresbefore actual deploymentwill be takenduring this time including programmingthe data acquisition probes,acquiring a sonardevice,preparingfield equipment,and accumulatingsupplies. April throughMay The preliminary deploymentwill be conductedduring this time. Prior to, during, and following the oxygenationprocess,datawill be collectedas previouslyoutlined. From this data,the volume, duration,and concentrationof the oxygenplume and the oxygenatoroperatingsetpoints will be examined. May throughJuly: During this period, the two deploymentswill be conducted.As aforementioned, oxygenationand fish countanalysiswill be conductedduring this time. July -October Analysesof oxygenationand fish responsewill continue. The literature review and economicanalysiswill be prefonIled. Resultswill be compiledand examined,and conclusionsdrawn. An article on the projectwill be written hopefully to be included in a peer-reviewedpublication. References Ricklefs, R.E. andMiller, G.L. EcologyFourth Edition. W.H. Freemanand Company, c2000 Chambers,P.A. andMill, T. DissolvedO.xygen, Fish and Nutrient Relationshipsin the AthabascaRiver. Northern River BasinsStudySynthesis,1999,ReportNo.5. Phang,S and Mukherjee,T. K. Rile ofAlgae in Livestock-FishIntegrated Farming Systems.Proceedingsof the FAO/IPT Workshopon IntegratedLivestock-Fish ProductionSystems,Dec. 16-20, 1991,Institute of AdvancedStudies,University of Malaya, Kuala Lumpur, Malaysia. Deas,M.L. and Orlob G.T. AssessmentofAlternativesfor Flow and Water Quality in the Klamath River belowIron GateDam. California Centerfor Environmentaland WaterResourcesEngineering,Nov1999, ReportNo. 99-04. United StatesEnvironmentalProtectionAgency,DevelopmentofDissolved Oxygen CriteriajorFreshwaterFish. Ecological ResearchSeriesEPA-R3-73-019, Feb1973. Osborn, G. Scott, Interpersonal Communication, 2004
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