Analytical and Bioanalytical Chemistry
Electronic Supplementary Material
Efficient method for determination of methylene blue dye in water samples
based on a combined dispersive solid phase and cloud point extraction using
Cu(OH)2 nanoflakes: central composite design optimization
Farzin Nekouei, Hanieh Kargarzadeh, Shahram Nekouei, Farzaneh Keshtpour,
Abdel Salam Hamdy Makhlouf
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Table S1 Methylene Blue characteristics and properties
Alternative names
CAS registry number
Chemical formula
Molar mass (g mol-1)
Appearance
Maximum wavelength (λmax)
3,7-bis(Dimethylamino)-phenothiazin-5-ium chloride
C.I. Basic Blue 9; Methylthioninium Chloride
61-73-4
C16H18N3SCl
319.850
Dark blue solid
664 nm
Molecular structure
Applications
leather
Coloring paper, cottons and wool, hair colorants,
Fig. S1 Absorption spectrum of Cu(OH)2 nanoflakes
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Fig. S2 FESEM images of the Cu(OH)2 nanoflakes
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Fig. S3 X‒ray diffraction (XRD) pattern of the Cu(OH)2 nanoflakes
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Fig. S4 (a) DLS histogram of the Cu(OH)2 nanoflakes size distribution by number.
(b) DLS statistics graph of the Cu(OH)2 nanoflakes by number
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Fig. S5 Adsorption Isotherm plot of the adsorbent
Fig. S6 FTIR‒ATR spectrum of Cu(OH)2 nanoflakes
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