Lesson Plan | 1 HOW DO SCIENTISTS COLLECT DATA? SAMPLING ACTIVITY Length: 40 minutes (20-minute video plus 20-minute activity and discussion) Age: Grades 6-12 Overview Through a recorded talk by MSU graduate student Madison Nixon, students will learn about agricultural ecology, sustainability, pest management and how climate change may impact the future of wheat production in Montana. The hands-on activity helps students understand how scientists sample for weed species, richness and diversity. Background A common difficulty in scientific research is sampling. A sample is something that is collected from a larger population. Samples provide information, called data. Sampling is the act of taking samples, i.e. counting elk in Yellowstone National Park, or collecting weed species in a wheat field. Scientists, such as ecologists, need to collect a lot of samples (often from large populations) in order to find answers to their research questions. Often times, scientists, such as ecologists, need to collect samples from large populations. It is often not practical or even possible to count all the members of a population, especially in large areas of land. A population can be so large, and sometimes spread out, that counting it would be like trying to count the number of grains of sand on a beach. To get around these problems, scientists take data from a smaller portion of the population. Samples are then used to make inferences about the entire population. Learning Outcomes: Objective 1: Help students understand why sound sampling methods, conducted within a realistic time frame, are crucial in achieving helpful data. Objective 2: Show students one or more ways to accomplish sampling weed communities and how to calculate weed species richness, density and diversity. MSU graduate student Madison Nixon coordinates a weed sampling activity at the Montana Teen Science Café. Photo by Suzi Taylor. Materials and Required Technology: • Varying amounts of at least three types of fake plants and/or flowers o Fake plants/flowers can be purchased at any craft store o 100+ “weeds” works well for this exercise in varying “species”. (The video example uses 150 total weeds from five different species) • A bag or box to put the “weeds” into • A way to calculate and record sampling results, e.g., pen, paper and a calculator • MS PowerPoint and projector Glossary of Terms: Species Richness: the number of species present • i.e. dandelion and creeping thistle = 2 species present Species Density: the amount of each species in a given area • i.e. 3 dandelions and 5 creeping thistles per square meter Species Diversity: each species’ percent of the total number This material is based on work supported by the National Science Foundation under Grant EPS-1101342. Any opinions, findings and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. • i.e. 3 dandelion + 5 creeping thistle = 8 total weedy plants • 3 dandelion/8 = 0.375 X 100 = 37.5% of the weeds are dandelions • 5 creeping thistle/8 = 0.625 X 100 = 62.5% of the weeds are creeping thistle Activity 1)Watch the Teen Science Café talk by Madison Nixon called “It’s Tough to be Wheat in a Changing Climate!” https://youtu.be/q1z-eSCuEk0 2)Briefly discuss why weeds are a topic of interest to people (i.e. yield loss in agriculture, invasive and/or noxious species) and why people may want to know species richness, density, and diversity of weedy species in a field or wildlife area. 3)Show students an example of a weed sampling method. Present the students with a hypothetical sampling situation related to weeds. e.g., There is a 10-square-mile wheat field and you are acting as researchers who need to get an idea of weed species richness, diversity, and density for this field. • Each student represents a sampling plot. Ask half of the students to blindly take 2 plants from the bag or box (low sampling density). Ask the other half of the students to take 8 plants from the bag or box (high sampling density). Note: the number of plants sampled (high, low, etc.) should be modified depending on total population size. • As a class, calculate species richness, density, and diversity for the two sampling densities. In the video example, 150 plants are included in the sample. Instructors do not need to use 150 plants, but should know the actual species richness, Suggested Alterations: The above activity can be modified to fit any time frame, space and age group. • With more time, add more levels of sample size i.e. low, med, high, etc. • Scatter the plants and flowers on the ground and have the students conduct the sampling in a pattern, using sampling frames (i.e. a frame of a known size) within a time limit. For example, ask them to sample using the “W” sampling pattern (in groups works best). “W” sampling pattern (orange) with 20 sampling areas (purple). (optional extension activity) diversity and density of the whole “weed population” prior to conducting the activity. 4)For the “low” and “high” sampling groups, compare their results to those of the actual population. 5)Discuss why more samples are usually more representative of the actual population, but how time and resource limitations often make it difficult to collect a sample size near that of the actual population. In conclusion, researchers must design a sampling method that will implement the largest sample size possible and within a reasonable time period and budget. • Make it more difficult by asking students to design and then implement their own sampling pattern (in groups works best), using sampling frames, within a time limit. The latter takes the lesson a step further to introduce the topic of non-bias sampling and why it is very rare to have the time and resources to sample all the weeds in an area of interest. • Instead of purchasing fake flowers/ plants, educators can download free “weed sampling activity cards” from http://eu.montana.edu/climb Lesson Plan | 2 LESSON PLAN AUTHOR Madison Nixon is a graduate student at Montana State University. She is pursuing her masters in Land Resources and Environmental Science. Madison has a BS in Sustainable Food and Bioenergy Systems and a BA in Latin American and Latino Studies. Teaching has long been a passion for Madison. She taught with the Bozone Ozone Greenhouse Bus, shared her masters research with kids through the Teen Science Café at Montana State University, and currently teaches afterschool Spanish to k-5 students in Bozeman, Montana. Examples of sampling frames. (optional extension activity) ABOUT THE MONTANA INSTITUTE ON ECOSYSTEMS The Montana Institute on Ecosystems is a community of scientists and partners that studies Montana’s complex ecosystems, including the impacts of climate change on ecosystems and the ways in which people and nature are interconnected. Formed in 2011 with funding from the National Science Foundation’s EPSCoR program, the IoE has offices at Montana State University and the University of Montana. Partners are located at other Montana University System campuses and Montana tribal colleges. The culminating research and outreach product of the EPSCoR / IoE will be the Montana Climate Assessment (MCA), which involves university researchers, decision makers, and other stakeholders with the goal of providing timely and relevant information for the citizens of the State. The inaugural MCA will focus on climate issues that affect agriculture, forests, and water resources in Montana. View more education and outreach resources at the Climate in My Backyard site: http://eu.montana.edu/CLIMB CLIMB is an educational outreach program serving K-12 teachers and informal educators. M O N T A N A N S F
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