observe
APPROVED
be inspired
reveal
HYDROFILTRAT MARINE LOTUS G
APPROVED
OXIDATIVE STRESS…
OUR WORST ENEMY FOR CELLULITE.
BE INSPIRED
Who said stress could be productive ?
In the case of our adipocytes stress in fact goes
with the production…of fat.
Without really noticing it, the external
environment is often hostile for our skin.
Repeated and daily exposure to exhaust
fumes, UV radiation, pesticides, cigarette
smoke…generates oxidative stress in our
skin. It causes the production of free
radicals, veritable cellular missiles which
attack our cells, oxidise proteins and
membrane lipids, degrade DNA, disturb
normal cell function, and accelerate their
ageing.
Lotus
maritimus
Still called “Pea
Lotus”
(Dragon’s
Tooth
or
Sea
Birdsfoot Trefoil) it
grows particularly in wet
regions near the sea
across all of western Europe
but it is also found inland,
including in the mountains up to 1600m
altitude.
It is a small vigorous plant with a woody tap
root and a horizontal stem which then
straightens up. Its flowers are golden
yellow giving it the name “Golden Slipper”,
or “Bride’s Slipper” in France.
Lotus has calming properties, in the
past it was given to reduce anxiety,
nervousness and muscular spasms.
Some use the flowers as a sleeping
draught. It was also taken to be the
magic plant which made Ulysses’
companions lose their memory in the
Odyssey.
OBSERVE
Adipocytes are generally considered to have the capacity to
store fatty acids. However they possess all the means
necessary to hydrolyse fats, this mechanism being called
Lipolysis. Lipolysis is induced by internal factors such as
adrenaline, but can also be amplified by external lipolytic such
as caffeine.
oxydative
stress
Fat storage
=
Healthy tissue
Fat storage
Fat Hydrolysis
Fat Hydrolysis
Ageing
Cellulite
For the more mystical amongst us,
Lotus
corniculatus
assists
psychic
powers
and
helps
clairvoyance.
An
ancient
Swedish tradition says that
Free radicals are implicated in fat storage through several ways:
young betrothed who put
Lotus flowers under their
• Alteration of the working order of adipocytes which become insensitive to
pillows on Midsummer’s
lipolytic agents (whether produced by the organism or supplied by slimming
Night will have revealed to
treatments)
them the face of their
• Stimulation of the production of prostaglandins, especially PGE2 which favours
fiancé.
the production of VEGF, the development of blood vessels and therefore the
supplying of oxygen and fatty acids to adipocytes.
• The decrease in the synthesis of adipokin, especially adiponectin, a molecule
responsible for the limitation of the expansion of adipose tissue.
At this stage, the adipocytes lose their lipolytic activity and continue to store fats
without ever hydrolysing them.
After several years research on the action of free radicals, CODIF Recherche et Nature has
developed Hydrofiltrat Marine Lotus G.
By protecting our adipocytes from oxidative stress and free radicals, it prevents loss of
functionality, delays ageing, restores lipolytic activity and boosts caffeine’s lipolytic effects.
Deprived of its best allies, cellulite disappears and our silhouette regains harmony and beauty.
MEMO ON CUTANEOUS BIOLOGY…
…THE LIPOLYTIC AGENTS
2 factors are mainly involved in lipolysis :
• cAMP (cyclic Adenosine MonoPhosphate), which is
synthesised
from
ATP
and
activates
lipase
by
phosphorylation,
• lipase which once it is activated catalyses triglyceride
hydrolysis into fatty acids and glycerol.
Triglycerides
PDE
Fatty
Acids
+
Glycerol
2
-
Lipase
β
AMPc
Adrenaline
+
1
Lipase
cAMP is therefore at the heart of lipolysis and is the first
target of agents which activate or inhibit this mechanism.
Phosphodiesterase (PDE) inhibits cAMP, while adrenaline
fixation on its beta receptor stimulates its activity.
One of the first targets of free radicals in the cell is the mitochondrion. When they are damaged,
mitochondria produce less ATP, the cAMP concentration falls and the lipolytic activity is therefore reduced.
By protecting adipocytes and their mitochondria from oxidative stress, Hydrofiltrat Marine Lotus G
maintains an optimal level of cAMP and restores the efficacy of lipolytic agents.
Effect of ageing and oxidative stress on lipolysis
In vitro, the lipolytic action of adrenaline on adipocyte cultures is recreated using isoproterenol or
noradrenaline. Like adrenaline, these two lipolytic agents fix on the β adrenergic receptor and stimulate
the hydrolysis of fatty acids. Caffeine, a lipolytic agent widely used in cosmetics, inhibits PDE, removing the
inhibition previously exercised by cAMP.
Effect of adipocytes ageing on the efficacy of lipolytic agents
Lipolysis (μg/million cell.hour)
6
Response to lipolysis
( μM fatty acid liberated)
1200
1000
-49%
800
-20%
-70%
600
-60%
400
Effect of oxidative stress on the efficacy of lipolytic agents:
5
Control
Stress
4
3
2
1
200
0
10-9
0
Isoproterenol
35-40 years old
Caffeine
40-50 years old
10-8
10-7,3
10-7
10-6,3
10-6
concentration in Noradrenaline (mol/L)
50-60 years old
Oxidising stress generated by addition of glucose oxidase
source CODIF International
Source INSA, Lyon
Oxidative stress and ageing are accompanied by a loss of adipocyte sensitivity to lipolytic
agents, and therefore a reduction in lipolysis.
Protecting the adipocytes from free radicals forearms them against any celllular dysfunctioning,
delays their ageing, and therefore maintains and reinforces the effectiveness of lipolytic agents
like caffeine in slimming treatment.
In-vitro Test :
antioxidant properties of Hydrofiltrat Marine Lotus G
ORAC Test
DPPH Test
12300
12000
160
140
DPPH
(μmol TE/L)
ORAC
(μmol TE/L)
10000
8000
6000
141
148
120
94
100
80
60
4000
40
2000
1669
20
396
0
0
Hydrofiltrat Marine
Lotus G
Cranberry
1%
Quercetin
1%
Hydrofiltrat Marine
Lotus G
Cranberry
1%
Quercetin
1%
•ORAC (Oxygen Radical Absorbance
Capacity) : overall measure of
antioxidant capability,
•DPPH : complementary test to
ORAC,
antioxidant
capacity
correlated to polyphenol content.
Reactions standardised in relation
to known concentrations of TROLOX
(the water soluble equivalent of
Vitamin E)
Cranberry and Quercetin are used
as positive controls.
TE = Trolox Equivalence
Hydrofiltrat Marine Lotus G has very high antioxidant effect.
In-vitro Test :
effect of Hydrofiltrat Marine Lotus G 2% on adipocyte lipolytic activity
Response to caffeine
Lipolytic Activity
(% of control)
Protocol :
Culture of mature adipocytes with or without oxidative
stress in presence or absence of Hydrofiltrat Marine
Lotus G 2% for 1 week.
Response to isoproterenol
250
225**
200
150
100
100
Incubation in presence of lipolytic agents ( isoproterenol
and caffeine)for 5 hours
101**
100
50
48
54*
Measure of lipolytic activity (amount of glycerol liberated
by lipolysis)
0
Without oxidative
stress
Oxidative stress
Oxidative Stress + Hydrofiltrat
Marine Lotus G
2%
Thanks to its strong antioxidant capacities, Hydrofiltrat
Marine Lotus G neutralizes free radicals, restores the
lipolytic activity of isoproterenol and boosts the efficacy
of caffeine.
*p<0.05 (student test)
**p<0.01 (student test)
Release of PEG2
(% of the control without oxidative stress)
In-vitro Test :
effect of Hydrofiltrat Marine Lotus G 2% on the release of PGE2
450
407
400
357
350
300
250
202**
200
The factor PGE2 (ProstaGlandin E2) is
released by fibroblasts under stressful
conditions.
PGE2 favours the production of VEGF
which stimulates the development of
blood vessels and therefore the supplying
of oxygen and fatty acids to adipocytes.
150
100
100
50
0
Without Oxidative
Stress
With Oxidative
Stress
Oxidative Stress
+ Hydrofiltrat
Marine Lotus G 2%
Oxidative Stress
+ Hydrofiltrat
Marine Lotus G 4%
Protocol :
Culture of fibroblasts with Hydrofiltrat
Marine Lotus G for 24 hours before and
after UV exposure.
** p<0.01 (Student test)
By protecting fibroblasts from oxidative stress, Hydrofiltrat Marine Lotus G limits the
secretion of PGE2 and therefore reduces the supplying of fatty acids to adipocytes.
Adiponectin synthesis
(% of the control without oxidative stress)
In-vitro Test :
effect of Hydrofiltrat Marine Lotus G 2% on the production and the
protection of adiponectin
Protocol :
Culture of adipocytes with Hydrofiltrat Marine Lotus G for 7
days, followed by UV exposure (oxidative stress).
160
Stimulating effect
141*
140
124*
120
100
Protective effect
100
83
89**
76*
80
60
40
20
0
Without
oxidative
stress
Without
oxidative
stress
+HMLG
1%
*p<0.05 **p<0,01 (Student Test)
Without
oxidative
stress
+HMLG
2%
With
oxidative
stress
With
oxidative
stress
+HMLG
1%
With
oxidative
stress
+HMLG
2%
Adiponectin is a factor released by adipocytes themselves,
which limits the expansion of the adipose tissue. Under the
effect of oxidative stress, its production is significantly
reduced.
First of all, Hydrofiltrat Marine Lotus G has a
stimulating effect on the synthesis of adiponectin,
then, thanks to its antioxidant capacities, it has a
protective effect when adipocytes are submitted to
oxidative stress.
These both complementary actions limit in a
permanently way the expansion of the adipose tissue.
HMLG=Hydrofiltrat Marine Lotus G
REVEAL
Clinical Test :
slimming effect of Hydrofiltrat Marine Lotus G 2%
Protocol :
• 15 volunteers between 30 and 50 years old
• 2 applications daily for 28 days on thighs, hips and buttocks
Evaluation of slimming effectiveness :
• fringe projection
• measure of loss in centimetres
• evaluation in a cosmetic of cellulite by macro-photography
FRINGE PROJECTION
Significant decrease in the perimeter of the
thighs observed by 73% of volunteers: -0.5%
on average (p<0.01) and up to -1,8%.
Significant decrease in the volume of the
buttocks by -4,4% (-0,9% on average).
Significant decrease in the volume of the
buttocks observed by 73% of volunteers:
-0.6% on average (p<0.01) and up to
-2.65%
D0
D0
D28
-2,65%
-1,8%
CENTIMETRE LOSS AND VISIBLE REDUCTION IN CELLULITE
Average and optimum centimetre losses
Hips
D0
*0,3
Buttocks
0,7
*0,3
A
statistically
significant
centimetre loss is measured
on the thighs, the buttocks
and the hips, accompanied by
a smoothing effect of the
orange peel aspect.
0,8
Thighs
**0,1
0,4
0
0,2
0,4
0,6
0,8
1
Centimetric lost (cm)
* p<0,001 / ** p<0,01
D28
The strong antioxidant effect of Hydrofiltrat Marine Lotus G protects adipocytes from the
negative effects of free radicals. It restores the lipolytic capacity of fat cells, limits the supplying
of fats to adipocytes, controls the expansion of the adipose tissue and strengthens the slimming
properties of caffeine.
Used alone as a 2% solution or in combination with another slimming agent such as caffeine,
Hydrofiltrat Marine Lotus G removes unsightly curves, sharpens the silhouette and removes
cellulite.
INCI : Glycerine (and) Water (and) Lotus maritimus extract
Land plant origin
Hydrosoluble active ingredient
in-vitro Test
in-vivo Test
APPROVED
ECOCERT validation
Controled management program of
natural resources
HYDROFILTRAT MARINE LOTUS G
ANTIOXIDANT-SLIMMING
• Anti-free radical activity – Anti-inflammatory activity
• Restoration of lipolytic activity
• Stimulation and protection of adiponectin
• Reinforcement of caffeine efficacy
• Reduction in buttock and thigh volume
• Elimination of orange peel effect
Cosmetic Activities
INCI
Glycerine (and) Water (and) Lotus maritimus extract
% concentration recommended
2%
Technical department : Codif Recherche & Nature
[email protected]
Commercial department: Codif Recherche & Nature
[email protected]
70, rue du Commandant l’Herminier - BP11709 - 35417 Saint-Malo cedex - FRANCE
Tel : +33-2-23-18-31-07 Fax : +33-2-23-18-31-01
www.codif-recherche-et-nature.com
All our wastewater is processed and purified using filtrating gardens.
A succession of airtight horizontal and vertical filters is used to depollute wastewater by the
roots of plant species chosen for their draining properties.
Iris, water mint, flowering rush, willows etc… now form part of the image of the brand which is
recognized for its commitment to the preservation of natural resources
RPDOC087GB
CODIF Recherche et Nature is committed to sustainable development.