Home › Products › Histone H3.2 Human, Recombinant
Histone H3.2 Human, Recombinant
Catalog #
M2506S
Size
100 μg
Concentration
1 mg/ml
Price
$75.00
Qty
1
Categories: Histones Products
Applications: DNA & Protein Methylation, Histones & Nucleosome Assembly
Product
Information
FAQs &
Tech Tips
Protocols &
Manuals
Other Tools &
Resources
Description
Related Products
References
Quality &
Safety
Legal
Information
Properties and Usage
Notes
Description
Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the
histone octamer. It is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be
important in gene regulation. Histone H3.2, an H3 variant that is found in all eukaryotes except budding yeast, is replication dependent and
is associated with gene silencing.
Protein Sequence: ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP ATGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL
VREIA QDFKT DLRFQ SSAVM ALQEA SEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA (Genbank accession number:
Q71DI3)
ESI-TOF Analysis of Histone H3.2 Human, Recombinant
H3.2 SDS-PAGE analysis of Histone H3.2 Human, Recombinant
Lane 1 & 7: NEB Protein Ladder (NEB #P7703).
Lane 2 through 6: 0.5, 1.0, 2.0, 5.0, 10.0 μg Histone H3.2 Human, Recombinant
Product Source
An E.coli strain that carries a plasmid encoding the cloned human histone H3.2 gene, HIST2H3A or HIST2H3C. (Genbank accession
number: BC130637)
Properties and Usage
Storage Temperature
-20°C
Storage Conditions
20 mM sodium phosphate
300 mM NaCl
1 mM DTT
1 mM EDTA
pH 7.0 @ 25°C
Related Products
Companion Products
Histone H2A Human Recombinant
Histone H3.1 Human, Recombinant
Histone H2B Human, Recombinant
Notes
1. 1 mg/ml, 66 µM is calculated using the molar extinction coefficient for Histone H3.2 (3960) and its absorbance at 280 nm (4,5). 1.0
A280 units = 3.9 mg/ml
2. Synonym: Histone H3/m, H3/o
3. Gene Synonym: H3F2, H3FM
References
1.
2.
3.
4.
5.
Kornberg, R.D. (1977). Annu. Rev. Biochem.. 46, 931-954.
van Holde, K.E. (1989). Chromatin. 1-497.
Hake, S.B. et al. (2006). J. Biol. Chem.. 281, 559-568.
Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem.. 182, 319-326.
Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
FAQs
Tech Tips
FAQs
1.
2.
3.
4.
Are the histones fusion proteins or tagged proteins?
Do the histones need to be reconstituted?
What are the recommended histone storage conditions?
Can the histones be used as substrates for protein modification enzymes? Which ones?
Tech Tips
After thawing on ice, mix well by pipetting the solution up and down. Do not centrifuge.
Datacards
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
M2506Datasheet-Lot0021206
M2506Datasheet-Lot0021211
M2506Datasheet-Lot0021308
M2506Datasheet-Lot0021312
M2506Datasheet-Lot0021404
M2506Datasheet-Lot0021408
M2506Datasheet-Lot0021412
M2506Datasheet-Lot0021508
M2506Datasheet-Lot0021512
M2506Datasheet-Lot0021604
M2506Datasheet-Lot0021608
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Quality Control
Datacards
Safety Data Sheet
Quality Control
Quality Control Assays
The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product.
Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting
Documents section of this page. Further information regarding NEB product quality can be found here.
Endonuclease Activity (Nicking):
The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to
the nicked form is determined by agarose gel electrophoresis.
Exonuclease Activity (Radioactivity Release):
The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4
hours the exonuclease activity is determined by the % release of radioactive nucleotides.
Histone Modification (Radioactivity Incorporation):
The Histone is tested in a reaction containing a specific Methyltransferase and radiolabeled SAM. After incubation the number of
pmoles of methyl group per unit of Methyltransferase transferred to the Histone is determined by radioactive incorporation.
Molecular Weight Determination (Mass Spectrometry) :
The molecular weight of the product is determined using mass spectrometry.
N-terminal Protein Sequencing :
Protein identity is confirmed using Edman Degradation to sequence the N-terminus of the intact protein.
Protease Activity (SDS-PAGE):
The product is tested for protease activity by incubation with a standard mixture of proteins resulting in no detectable
degradation of the proteins as determined by SDS-PAGE.
Safety Data Sheet
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Histone H3.2 Human, Recombinant
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
M2506Datasheet-Lot0021206
M2506Datasheet-Lot0021211
M2506Datasheet-Lot0021308
M2506Datasheet-Lot0021312
M2506Datasheet-Lot0021404
M2506Datasheet-Lot0021408
M2506Datasheet-Lot0021412
M2506Datasheet-Lot0021508
M2506Datasheet-Lot0021512
M2506Datasheet-Lot0021604
M2506Datasheet-Lot0021608
Legal and Disclaimers
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc
(NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain
additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes
in humans or animals.