SHORT COURSE: CARBOHYDRATES AND FOOD STRUCTURE DESIGN COLOFON Redactie/productie: TNO, Marketing & Communications Ontwerp en opmaak: Grafisch Ontwerp Pi&Q, Zeist Druk: De Swart, Den Haag © TNO 2015 2 PROGRAM Tuesday, November 10th 09.00 Coffee and reception 09.30 Opening: Introduction to the course Dr. Stefano Renzetti, Prof. Bruce Hamaker 09.45Carbohydrate structure and starch modification: chemical, physical and enzymatic Dr. Yuan Yao (Whistler Center) 11.15 Coffee/tea break 11.45 Novel physical modification technologies Dr. Jerome Diaz (TNO) 12.45Lunch 13.45 Lactobacillus Polysaccharides: Synthesis and Degradation Prof. Lubbert Dijkhuizen (University of Groningen/Carbohydrate Competence Center) 14.25 Carbohydrate digestion and sustainable energy Prof. Bruce Hamaker (Whistler Center) 15.40 Coffee/tea break 16.00 Prebiotics: The Fall & Rise of Health Claims Dr. Elaine Vaughan (Sensus) 16.30 Prebiotic modulation of the human gut microbiota Dr. Frank Schuren (TNO) 19.00 Course dinner Wednesday, November 11th 08.30 Coffee and reception 09.00 Welcome and introduction day 2 Dr. Stefano Renzetti, Prof. Bruce Hamaker 09.10 Physical and Rheological Properties of Biomaterials Dr. Osvaldo Campanella (Whistler Center) 10.40 Coffee/tea break 11.10Biopolymers interactions Dr. Owen Jones (Whistler Center) 12.40Lunch 13.30 Functionality of starch and starch derivatives in food Dr. Piet Buwalda (Avebe/University of Wageningen) 14.15 Food structure and texture design: towards healthy formulations (part 1) Albert Jurgens (TNO) 15.00 Coffee/tea break 15.15 Food structure and texture design: towards healthy formulations (part 2) Albert Jurgens (TNO) 15.45Linking innovative food structure design with dietary guidelines, consumer’s views and sustainability Jan-Willem van der Kamp (TNO) 16.15 Closing remarks 3 Dr. Stefano Renzetti obtained a MSc in Food Sciences and Technology from the University of Bologna, Italy, in 2000 and later specialized in Cereal Sciences obtaining his PhD at the University College Cork, Ireland, in 2009. His PhD research focused on the functionalization of gluten-free flour for bread-making purposes with enzymatic treatments. Prior to undertaking a PhD program, Stefano worked as a researcher in food microbiology at Unilever Research Headquarters in the Netherlands (2000–2002) and as Quality Assurance Manager for two well-established Italian companies (2002–2005). Since 2009, Stefano is working at TNO as senior scientist within the e  xpertise group Functional Ingredients. His current activities focus on reformulation of bakery products and snacks for nutritional and clean label purposes, t exture and structure design of food products, physical properties and p  hysical stability of food and moisture migration in food products. Stefano also participated in an open innovation network, i.e. the Top Institute of Food and Nutrition (The Netherlands), where he was appointed as scientist for projects regarding water migration in cellular solid food and powders, as well as fat structuring. 4 ←← Return to Program CARBOHYDRATE STRUCTURE AND STARCH MODIFICATION: CHEMICAL, PHYSICAL AND ENZYMATIC Carbohydrates, such as starch, have broad and important applications in the modern food industry. In this 90-minute lecture, we will review some basic concepts related to carbohydrate structure, in particular the structural properties of carbohydrate polymers. For example, we will discuss carbohydrate polymers based on their charges, molecular weights, and shapes. The basic structural properties of these biobased macromolecules have governing effects on their functionalities and performances in food. Specifically, we will use starches and their alphaD-glucan analogues to highlight the structure- function relationships of carbohydrate polymers. Either as natural food components or as added ingredients, starches and their derivatives affect the nutritional value, textural characteristics, and shelf life of food. ←← Return to Program Dr. Yuan Yao is an Associate Professor in the Department of Food Science of Purdue University and affiliated with the Whistler Center for Carbohydrate Research. He holds degrees of Polymer Science, Chemical Engineering, and Food Science, as well as post-doc training in plant molecular biology. He has years of industrial experience in the food ingredients industry and joined Purdue University in 2005. At Purdue, his primary researches focus on carbohydrate-related biomaterials and their applications in food, nutrition, and medical areas. Through genetic, chemical, physical, and enzymatic modifications, he has been developing new materials from starches and their analogues, not only as functional food ingredients, but also as novel pharmaceutical excipients. His researches have been supported by NSF, USDA, and the industry. To optimize the performances of starches, various approaches have been used, including genetic, chemical, physical, and enzymatic modifications. These modifications change the fine structure of starch molecules or bring functional groups to affect the behaviors of glucan molecules in various relevant environments, such as in low-temperature storage and high-temperature, high-shear food processing. Through this lecture, the audiences are expected to form an overall concept of structure-function relationship of carbohydrates, in particular starch. W 5 NOVEL PHYSICAL MODIFICATION TECHNOLOGIES The rise in public awareness regarding the wholesomeness of the foods they consume have resulted in a shift towards the use of natural and sustainable ingredients by food producers and food product developers. However, the functionality requirements for many food product applications are not easily met by existing natural ingredients. In the past, chemical modification as a strategy for conferring improved ingredient functionalities have proven to be successful. For example, starch functionality have been extended by various chemical modifications. Then again, consumer acceptance for chemically modified ingredients have decreased. As a response, the development of green, sustainable modification technologies have grown. 6 Nowadays, food companies and product d  evelopers will opt to use sustainable green ingredients over those that are chemically modified. From this viewpoint, the next generation of specialty food ingredients will come from the physical m  odification of polysaccharides. These polysaccharides include starches and hydrocolloids like pectins, mannans, and gums among others. They also include h  emicellulose such as arabinoxylan, and x yloglucan among others. Novel physical modification technologies such as super-heated steam (SHS) technology, among others is a good example of an alternative process technology to achieve the desired technological properties of polysaccharides without the need for chemical modification. This presentation will highlight specific cases of physical modification of various polysaccharides and how their f unctionalities may be altered. W Jerome Diaz is a scientist at TNO’s functional ingredients group since 2010. His work focuses mainly on elucidating carbohydrate structure and function relationships with emphasis on developing techno-functional carbohydrate based ingredients. His current projects include clean labelling, 3D food printing, among others. He is also involved in TNO Early Research Programs on developing technologies for early life development and healthy aging. Jerome obtained his doctoral degree in food science from the University of California, Davis as a Fulbright fellow and served as a postdoctoral scientist at the University of Copenhagen before joining TNO. ←← Return to Program Prof. dr. Lubbert Dijkhuizen is professor of Microbiology at the University of Groningen. He initiated many research projects into life processes in micro-organisms and r elevant industrial and other applications of this fundamental knowledge. The main focus in this research is on the ability to use micro-organisms and enzymes in biotechnological processes, in particular in the conversion of carbohydrates. He is Head of the Microbiological Physiology group. Dijkhuizen is one of the initiators and joint founders of the Carbohydrate Competence Center (CCC), a public-private cooperation among 19 businesses and 6 knowledge institutions. Research is being conducted in 24 projects on carbohydrates that play an important role in Nutrition and Health. ←← Return to Program LACTOBACILLUS POLYSACCHARIDES: SYNTHESIS AND DEGRADATION The gastrointestinal tract of vertebrates is inhabited by many bacteria that play an important role in their physiology of these animals. Among these bacteria is Lactobacillus reuteri for which n  umerous beneficial effects have been reported against different g astrointestinal pathogens. L.reuteri has gained GRAS status and is widely used in food and feed industries. The outmost layer covering these bacteria is composed of high molecular weight α-glucans produced by glucansucrases. These are large e  xtracellular enzymes (≈160 kDa), which use a simple substrate such as sucrose to synthesize the α-glucans and/or small oligosaccharides (if suitable acceptor molecules are available). Dextran is the most well-known type of alpha-glucan has been characterized in detail. It has found a  pplication in various fields, most clearly in pharmacy, in m  edicine, and in bakery. Due to the vast p  otential industrial applications of the large α-glucans p  roduced, g lucansucrases have been extensively studied. To date, over 50 different glucansucrases have been classified in Glycoside Hydrolase family 70. Glucansucrase enzymes are found extracellularly, only reported to occur in lactic acid bacteria, members of the genera Lactobacillus, Streptococcus, Leuconostoc, and Weissella. Four different glucansucrase genes, gtf180, gtfML1, gtfA and gtfO have been isolated from four different Lactobacillus reuteri strains, LB 180, LB ML1, LB 121 and LB ATCC 55730. These genes encoded a dextransucrase [dextran, α-(1→6) glucosidic linkages], a mutansucrase [mutan α-(1→3) glucosidic linkages], a reuteransucrase containing 50% α-(1→4) linkages and a reuteransucrase with 70% α-(1→4) linkages, respectively. These glucansucrase enzymes are highly similar at the amino acid level (65% identity and 70% similarity) but nevertheless synthesize very different α-glucan products. Clear understanding of the structural features in glucansucrase enzymes that determine the nature and ratio of glucosidic linkages synthesized is sought from high resolution 3D structures and may allow production of tailor-made poly- and oligosaccharide products suitable for diverse (food) applications. In this lecture the results of our glucansucrase enzyme and product characterization will be reviewed. W 7 CARBOHYDRATE DIGESTION AND SUSTAINABLE ENERGY Digestible carbohydrates provide direct energy to the body through their digestion by the α-amylase and α-glucosidase enzymes to release glucose, fructose, and galactose. These include starch and starch-based products, sucrose, lactose, and some other less common α-linked oligoglucans. Their rate of digestion affects blood glucose, or glycemic, response profiles which has health implication. Digestion rate also relates to location of sugar release in the small intestine which may also be important due to certain physiological feedback controls affecting the satiety center of the brain and control of stomach emptying rate. There are various factors, both extrinsic to the food itself and intrinsic to the body, that influence carbohydrate digestion rate. A current understanding of the field will be presented as well as strategies for extending carbohydrate digestion rate for sustainable energy. W 8 Bruce R. Hamaker is Distinguished Professor of Food Science at Purdue University, West Lafayette, Indiana. He also holds the Roy L. Whistler Chair and is Director of the Whistler Center of Carbohydrate Research. He obtained his undergraduate degree in biological sciences from Indiana University; his graduate studies were in human nutrition (M.S.) and food chemistry (Ph.D.) from Purdue University, and post-doctoral study at the Instituto de Investigacion Nutricional in Lima, Peru (supervisor, George Graham, Johns Hopkins University). He was in the US Peace Corps in Liberia, West Africa from 1977–1979. He has over 160 refereed journal publications in food science, human nutrition, biochemistry and broad spectrum journals, as well as numerous book chapters. He has advised over 50 M.S and Ph.D. students and nearly twenty post-doctoral scientists. Dr. Hamaker’s research is mainly known in the area of food carbohydrates and proteins with applications related to topics of health and wellness. In this regard, he has a number of clinical and nutrition group collaborations. He has been and continues to be active in international research collaborations in Africa and Asia. ←← Return to Program PREBIOTICS: THE FALL & RISE OF HEALTH CLAIMS ß(2,1)-fructans, typically inulins, extracted from roots of the chicory plant are at the basis of the term ‘prebiotic’—a concept that was introduced in 1995. Prebiotics may be defined as ‘a selectively fermented ingredient that results in specific changes in the composition and/or activity of the gastrointestinal microbiota, thus conferring benefit(s) upon host health’, although the concept definition is continually evolving1. Fructans of varying chain length are widely used in all food market segments for their technological and nutritional properties, including sugar/fat replacement, low caloric value, amongst others. They modify the gut microbiota in infants to adults, notably eliciting a bifidogenic effect with high short chain fatty acid levels; more recent data show that other gut species associated with anti-inflammation and anti-obesity are also stimulated2,3. ←← Return to Program Dr. Elaine E. Vaughan obtained her BSc honours in microbiology including nutrition, cell biology and immunology, and received her PhD at University College Cork, Ireland in the field of dairy microbiology and carbohydrate metabolism. She was awarded a Marie-Curie grant for post-doctoral research at NIZO Food Research, the Netherlands, on molecular biology of dairy cultures and subsequently went to Nestle Research Center, Switzerland, contributing to probiotic research. Upon return to the Netherlands, she became assistant professor in Molecular Microbial Ecology at Wageningen University and senior scientist at Top Institute Food & Nutrition managing a group with PhDs and post-docs in the field of gut microbiomics including pro- and prebiotic research. She later joined Unilever R&D in various roles translating scientific & technology developments into business needs and delivering food concepts; she also coordinated a European Union project on polyphenols and the human microbiome. Elaine regularly reviews scientific work, published extensively in journals, and edited the first book dedicated to Gastrointestinal Microbiology (2006). In 2014 she joined Sensus B.V. (Royal Cosun) leading scientific and regulatory affairs; she especially defines scientific research to strengthen the beneficial effects of prebiotic chicory root fibre on human health and building evidence to underpin health claims. Today metagenomics is providing new insights in gut microbiota functionality on human health but this research is still in its infancy. Health claims on foods require solid scientific support. In Europe, novel regulation for nutrition and health claims was introduced in 2006 which may be the strictest system globally; the process requires submission of a dossier with scientific material supporting the health claim to the European Food Safety Authority and subsequent authorization by the European Commission. While giving legal certainty, it has been a significant adjustment for the food industry; the majority of previously allowed health claims were rejected due to insufficient ingredient characterisation or underpinning health evidence. The food industry has used different strategies to overcome this, and importantly scientific support for health benefits following the guidance being developed by the EC is slowly supporting recovery of health claims. As example, nutritional caloric and fibre claims are used for inulin and oligofructose fructans, and more recently positive opinions for health claims of inulin/oligofructose have been published by EFSA for glycaemic response. W References 1. Hutkins et al in press 2. Ramirez-Farias et al., 2009 3. Everard et al., 2013 9 PREBIOTIC MODULATION OF THE HUMAN GUT MICROBIOTA The important role micro-organisms play in human health has gained a lot of attention recently. Microbiota composition has been related to many different human diseases and modulation of these bacterial populations has been recognized as a novel health target. Our nutrition serves not just as an energy supply for the human body but also for the human microbiota. Therefore nutritional intervention offers unique possibilities to influence microbiota composition and functionality, especially in preventive approaches and possibly in early stages of disease development. Well-known examples of such interventions are probiotic bacteria and prebiotics, oligosaccharides which can selectively stimulate the growth of Bifidobacteria, a group of bacteria considered to have a positive effect on health. Novel research shows that prebiotic activity can be derived form many different nutritional; s  ources and some results of our work in this area will; be presented. A more challenging line of research focuses on influencing other groups of bacteria within the microbiota, either through specific s  timulation or through specific inhibition. Technologies which enable the selection of ingredients with such properties will be presented as well as results obtained with these technologies. W Frank Schuren received his PhD at Groningen University in 1987 on the molecular biological analysis of fungal development. After a postdoc on genetic transformation systems in filamentous fungi at the same University and a postdoc at the ETH Zurich in Switzerland he joined TNO in 1996. At TNO he started the implementation of microarray technology in applied research which led to successful implementation in applied microbial research. These include novel diagnostic tools which enable the discrimination between closely related bacterial strains (such as the Legionella chip) and the analysis of complex microbial p opulations (such as the intestinal and vaginal microbiota). Also tools for analyzing the immediate responses of microbes or microbial populations towards multiple stressors have been developed. These tools allow for generating p  redictive models of behaviour of microbes. Currently Frank is lead scientist for the novel TNO research program on Personalized Food for Health with a special e  mphasis on Early Life. 10 ←← Return to Program PHYSICAL AND RHEOLOGICAL PROPERTIES OF BIOMATERIALS This section will describe basic principles to measure and calculate physicochemical properties of bio materials with emphasis in carbohydrates and other food components. It will provide tools to identify parameters associated to unit operations involved in bioprocesses, as well as gain knowledge of main factors that determine the values of these physical chemical properties. The use of basic principles of rheology to correlate mechanical properties with quality parameters of biomaterials will be also discussed. W ←← Return to Program Dr. Osvaldo H. Campanella is a Professor of Food and Biological Engineering at the Biological Engineering Department and the Whistler Carbohydrate Research Center, Purdue University. He has specialized in food extrusion, rheology of complex fluids, and food and food and biological processing engineering. At Purdue University Osvaldo Campanella has focused his research on Extrusion, Rheology, Heat Transfer Modeling, and Modeling of Cereal Processes. He is also actively involved in research related to new methods to characterize the formation of complex compounds using novel acoustic/ultrasound techniques. Dr. Campanella has published more than 140 scientific articles related to rheology, e  xtrusion, food engineering, thermal processing and characterization of biomaterials using acoustic methods and has authored book on extrusion technology. Dr. Campanella was awarded the Best Engineering Teacher Award in Agricultural and Biological Engineering in 2000–2001 and 2011–2012, and named Purdue University Faculty Scholar in 2007 and best graduate student mentor in 2014–2015. He has been associate editor of a number of specialized journals and has written chapters in a number of books related to food processing, rheology, extrusion. 11 BIOPOLYMERS INTERACTIONS Dr. Owen Griffith Jones, Ph.D., is an Assistant Professor of Food Science at Purdue University and a member of the Whistler Center for Carbohydrate Research (WCCR). He received his B.Sc. degree in Food Science with a minor in Chemistry from the Ohio State University and his doctorate from the University of Massachusetts in the laboratory of Food Biopolymers and Colloids Research. Dr. Jones then spent two years at Eidgenössiche Technische Hochschule in Zürich, Switzerland, as a post-doctoral researcher in the laboratory of Food and Soft Materials. He joined Purdue University and the WCCR in 2011. Dr. Jones’ research focuses on colloidal s  tructures formed from food-grade biopolymers, interactions between biopolymers in solution, protein-based emulsion systems, and the d  evelopment of materials using c olloidal assemblies. 12 Behaviour of biopolymer-based food systems is related to the physical characteristics of the individual biopolymers and their tendency towards interactions with other components in solutions, whether that interaction is associative or repulsive. The most important interaction of a biopolymer in terms of its physical behaviour is with solvent, and so the polymer attributes contributing towards s  olubility and dispersion in solution will be discussed for theoretical and practical systems. Interactions between biopolymers in ternary mixtures will be further defined as either incompatible or associative, with discussion of the underlying mechanisms. Examples of representative phase diagrams will be provided for both incompatible and associative s  ystems, and impacts of such phase separations on the rheological and visual properties of suspensions will be discussed. W ←← Return to Program FUNCTIONALITY OF STARCH AND STARCH DERIVATIVES IN FOOD Starch is an abundant natural source of polymeric material. It is produced by plants where it serves as an energy storage medium. Mankind has used this material for improving life, not only as part as the energy supply to the body, but also to improve the perception and appreciation of foods, to make paper, to improve sizing of yarns, paste wallpaper. In short improve life in a plethora of fields. In this lecture the structure of starches, the difference between different botanical sources and the impact of modification will be discussed. Two special cases will be discussed in detail: Crosslinking of starch and starch gelling. For Crosslinking a theoretical model will presented. For gelling general principals will be discussed as well as recent developments on strong gelling agents based on starch. W In order to understand the functionality of starches in food applications and processes, understanding of starch and starch derivatives structure is key. Moreover, how this structure works out for the functionality of the starch in a given application will generate solutions for every day manufacturing. Piet Buwalda studied at the University of Groningen where he earned a PhD in chemistry. He started working at AVEBE in 1989 doing basic research into etherification reactions of starch. Later on he started working in numerous product developments in food and industrial applications. Soon after he was involved in integrated chain approached ranging from potato growing through starch variation to amylomaltase treated starch. After heading the Food Innovation center for 10 years, he became the Starch Technology Manager for the AVEBE total. He has been involved in game changing innovations such as the ELIANE™ range and the Etenia™ gelling starches His special scientific interest is in enzyme m  odified starches and crosslinking reactions of granular starch. He holds 21 independent patents and has published 25 publications. Recently, he has been appointed as an Associated Professor at the University of Wageningen. ←← Return to Program 13 FOOD STRUCTURE AND TEXTURE DESIGN: TOWARDS HEALTHY FORMULATIONS We enjoy our food, not only because of its taste, but also due to its texture. A wide range of factors determine our preference for certain textures, and the understanding of these is the intricate field of psycho-perception. Whatever the reasons for enjoying foods may be, we can conclude that most foods we like have complex structures on different length scales and even multiple textures. As an example the contrast between a crispy crust and a soft inner core in snacks and also in bread is very much liked. Over the last years many consumers have become aware that their choice of food and eating habits have an impact on health. The increasing demand for healthy products, which are still tasty and have good textural quality, is the driving force for reformulation towards reduced salt, sugar and fat contents and increased levels of fibre. Most food products are composed of combinations of various carbohydrates, proteins, lipids and water. Depending on their nature these components can interact with each other in several ways to form structures, either on short or long length scales. Changing ingredients will cause changes in the final structure, which in many cases has a negative effect on perception. 14 Moreover, different ingredients can form m  ultiple phases, such as e.g. sugars which may e  ither crystallize or form amorphous structures. Understanding the physics behind the formation of these structures in complex compositions and the relations with the final texture leads the way to find routes for the design of high quality, healthy formulations. By means of a number practical examples it will be shown what r outes can be followed in the case of reformulation. Obtaining a full understanding of the interactions t aking place in complex compositions is hardly p ossible. Moreover, quality parameters of the products are frequently based on qualitative sensory evaluations. It will be shown how statistics can be of help to find the relevant physico-chemical factors behind the key quality parameters. Albert Jurgens followed his education in colloid science and chemical thermodynamics at the university of Utrecht. For a number of years he worked as a scientist at Unilever Research in Vlaardingen on the relationship between microstructure and rheology of structured liquids and semi-solids. After applying his knowledge, at Unilever, to formulating liquid detergents he joined TNO in 1991 to work in the Cereals and Bakery Institute as scientist in the area of food physics. In the following years he widened his knowledge of application of food physics towards different foods areas such as oils and fats, meat products and snacks. Today he is senior scientist in the department of Functional Ingredients of TNO at present working mainly on projects related to reformulation. The next step is to set up hypotheses for the formation of the food structure and the final t exture characteristics based on physico-chemical m  odels, and find the relationships with the s  pecific ingredient characteristics. Different physical m  ethods, such as rheology, thermal- and structure analysis are of help to deliver parameters fitting into such models. From the relationships found guidelines can be set up in the form of a ‘toolbox’ to be used by the industry for the development of reformulated products. W ←← Return to Program LINKING INNOVATIVE FOOD STRUCTURE DESIGN WITH DIETARY GUIDELINES, SUSTAINABILITY AND CONSUMER’S VIEWS Dietary guidelines based on comprehensive scientific reports are issues and updated regularly by countries and groups of countries. Recent examples are the Scientific Report of the 2015 Dietary Guidelines Advisory Committee (USA, USDA, February 2015) and the comprehensive (36MB) Carbohydrates and Health report of the Scientific Advisory Committee on Nutrition (England, July 2015). Common elements in recent reports and recommendations are: –Energy from intake of carbohydrates ~50% of total energy intake. –Reduced consumption of added mono- and disaccharides to preferably 5 % of total energy intake. –Increased intake of dietary fibre for adults to ~30g/day, preferably from natural sources and not as added purified fibre, and highlighting the benefits of cereal grain based fibres. –No clear recommendations for foods with low glycemic index or load. Note: the EU has authorized ‘reduction of post-prandial glycaemic responses’ health claims for a range of fibres. ←← Return to Program Current guidelines mention bacteria only in relation to food spoilage, but new documents start to consider potential health effects of pre-and probiotics, albeit not yet with positive conclusions. Interest in sustainability of food production and consumption has grown considerably In recent  roduct) years. The carbon footprint (kg CO2 eq./kg p of food products has now been calculated; levels of plant-based food products are much lower than of animal products. The 2015 USDA report recommends a shift in food patterns to a more p  lant-based diet both for improving health and for sustainability reasons, whereas in earlier versions only health was considered. Jan Willem van der Kamp has over 35 years experience in cereal and food research, biotechnology, research management and communication. In addition to his R&D positions in Unilever and since 1985 in TNO he served in ICC as President and currently as Chair of the ICC Academy, and in AACCI as International Director. He managed and p  articipated in a wide range of EU projects including HEALTHGRAIN 2005–2010), as leader of the technology transfer and communication activities and HealthBread (2012–2014), as coordinator. His recent activities focus on food and health related regulatory affairs, including issues related to potential health effects of fibre-rich diets, pre and probiotics and the differences in perception by nutritionists and microbiologists. Consumer’s views are increasingly influenced by soloist-‘experts’ with radical and unconventional dietary recommendations such as minimizing starchy carbohydrate intake (‘no part of primitive man’s diet’). These new and straightforward messages are eagerly communicated in all kinds of media, although most of their views are not supported at all by science. W 15 TNO.NL 15-7422 October 2015
© Copyright 2025 Paperzz