allelebiotech.com
Allele Biotechnology & Pharmaceuticals, Inc.
Alleleustrious pmWasabi-N
Recommended Use
(N mammalian expression)
mWasabi has been optimized for use with standard GFP/FITC filter
sets.
Features
Catalog Number:
Size: 10ug
ABP-FP-WNNCS10
Price:
$349.00
Introduction
ele
All ustrious pmWasabi-N is a mammalian expression vector with a multiple
cloning site for fusions to the N-terminus of mWasabi, also suitable for
ele
expression of mWasabi alone in mammalian cells. All ustrious mWasabi is
a monomeric green fluorescent protein that can be easily detected using
standard GFP filter sets. mWasabi may be used as a direct replacement for
EGFP or other GFPs for superior performance, and may be co-imaged with
blue and red fluorescent labels without substantial bleed-through.
• About 2-fold brighter than EGFP
•
•
•
•
•
•
Similar photostability to EGFP
Uses standard filter sets
Can be co-imaged with blue and red FPs or dyes
Mammalian expression vector ready to transfect your favorite cells
Low sensitivity to acidic pH (fluorescence pKa=4.3)
True monomer that will not aggregate or cause nonspecific
interactions
Reconstitution
10 µg provided in lyophilized powder form. Reconstitute with 10 µL of
nuclease-free water for a final concentration of 1 µg/µL.
Source
Storage
Engineered variant of mTFP1, originally derived from Clavularia sp. coral.
Store at -20°C or at -80°C for long-term preservation.
Human CMV Immediate-Early
Promoter (CMV Promoter)........1-589
Multiple Cloning Sites (MCS)....591-646
PCMV
pUC Ori
mWasabi.....................................653-1363
SV40 PolyA Signal.....................1529-1579
MCS
bla Promoter...............................2118-2222
pmWasabi
Ampicillin Resistance Gene......2207-3067
3940bp
pUC Origin..................................3216-3858
Upstream Sequencing Primer:
AMPR
SV40
Poly A
Universal CMV Promoter Primer
Downstream Sequencing Primer:
mWasabi
BLA
SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C
Human CMV Immediate Early Promoter
481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA
NheI
601 GACTGGATCC TCGAATTCGG TACCTAAAGC TTGCGGCCGC CTCGAGGCCA CCATGGTCAG
BamHI
EcoRI KpnI
HindIII NotI
XhoI Kozak Start
codon of mTFP1
661 CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC ATGAAGATCA AGCTGAAGAT
1321 GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG TAAGCATGCA GATCCACCGG
Stop codon of mTFP1
References: Ai H, Olenych SG, Wong P, Davidson MW, Campbell RE. Hue-shifted monomeric variants Clavularia cyan fluorescent protein: identification
of the molecular determinants of color and applications in fluorescence imaging. BMC Biology. 2008 Mar; 6:13. Shaner NC, Patterson GH, Davidson MW.
Advances in fluorescent protein technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. Ai HW, Hazelwood KL, Davidson MW, Campbell RE.
Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ,
Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and
applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods.
2005 2(12):905-09
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc.
allelebiotech.com
SEQUENCE
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
TAGTTATTAA
CGTTACATAA
GACGTCAATA
ATGGGTGGAG
AAGTACGCCC
CATGACCTTA
CATGGTGATG
ATTTCCAAGT
GGACTTTCCA
ACGGTGGGAG
GACTGGATCC
CAAGGGCGAG
GGAGGGCAAC
CGACGGCACC
CGACATTCTG
CATCCCCAAC
CTTCGAGGAC
CATCTACGAG
GGAGACCACC
GGGCGACGTC
GACCATCTAC
CATCGAGATC
GGCCCGCAAC
ATCTAGATAA
AAACCTCCCA
CTTGTTTATT
TAAAGCATTT
ACGCGTCCTG
CTACACTTGC
CGTTCGCCGG
GTGCTTTACG
CATCGCCCTG
GACTCTTGTT
AAGGGATTTT
ACGCGAATTT
GCGCGGAACC
ACAATAACCC
TTTCCGTGTC
AGAAACGCTG
CGAACTGGAT
AATGATGAGC
GCAAGAGCAA
AGTCACAGAA
AACCATGAGT
GCTAACCGCT
GGAGCTGAAT
AACAACGTTG
AATAGACTGG
TGGCTGGTTT
AGCACTGGGG
GGCAACTATG
TTGGTAACTG
TTAATTTAAA
ACGTGAGTTT
AGATCCTTTT
GGTGGTTTGT
CAGAGCGCAG
GAACTCTGTA
CAGTGGCGAT
GCAGCGGTCG
CACCGAACTG
AAAGGCGGAC
TCCAGGGGGA
GCGTCGATTT
GGCCTTTTTA
ATCCCCTGAT
TAGTAATCAA
CTTACGGTAA
ATGACGTATG
TATTTACGGT
CCTATTGACG
TGGGACTTTC
CGGTTTTGGC
CTCCACCCCA
AAATGTCGTA
GTCTATATAA
TCGAATTCGG
GAGACCACAA
GTGAATGGCC
AACACCATCA
ACCACCGCGT
TACTTCAAGC
AAGGGCATCG
ATACACCTCA
GGCTGGGACG
AAGATGAAGC
AGGGCCAAGA
CTGAACCACG
TCCACCGACG
CTGATCATAA
CACCTCCCCC
GCAGCTTATA
TTTTCACTGC
TAGCGGCGCA
CAGCGCCCTA
CTTTCCCCGT
GCACCTCGAC
ATAGACGGTT
CCAAACTGGA
GCCGATTTCG
TAACAAAATA
CCTATTTGTT
TGATAAATGC
GCCCTTATTC
GTGAAAGTAA
CTCAACAGCG
ACTTTTAAAG
CTCGGTCGCC
AAGCATCTTA
GATAACACTG
TTTTTGCACA
GAAGCCATAC
CGCAAACTAT
ATGGAGGCGG
ATTGCTGATA
CCAGATGGTA
GATGAACGAA
TCAGACCAAG
AGGATCTAGG
TCGTTCCACT
TTTCTGCGCG
TTGCCGGATC
ATACCAAATA
GCACCGCCTA
AAGTCGTGTC
GGCTGAACGG
AGATACCTAC
AGGTATCCGG
AACGCCTGGT
TTGTGATGCT
CGGTTCCTGG
TCTGTGGATA
TTACGGGGTC
ATGGCCCGCC
TTCCCATAGT
AAACTGCCCA
TCAATGACGG
CTACTTGGCA
AGTACATCAA
TTGACGTCAA
ACAACTCCGC
GCAGAGCTGG
TACCTCAAGC
TGGGCGTAAT
ACGCCTTCGT
ACCTGGAGGT
TCAGTTACGG
AGTCCTTCCC
TGAAGGTGAA
AGGGCGAGAA
CCTCCACCGA
TGCTGCTGGA
AGGCGGTGAA
ACAAGGACTA
GCATGGACGA
TCAGCCATAC
TGAACCTGAA
ATGGTTACAA
ATTCTAGTTG
TTAAGCGCGG
GCGCCCGCTC
CAAGCTCTAA
CCCAAAAAAC
TTTCGCCCTT
ACAACACTCA
GCCTATTGGT
TTAACGCTTA
TATTTTTCTA
TTCAATAATA
CCTTTTTTGC
AAGATGCTGA
GTAAGATCCT
TTCTGCTATG
GCATACACTA
CGGATGGCAT
CGGCCAACTT
ACATGGGGGA
CAAACGACGA
TAACTGGCGA
ATAAAGTTGC
AATCTGGAGC
AGCCCTCCCG
ATAGACAGAT
TTTACTCATA
TGAAGATCCT
GAGCGTCAGA
TAATCTGCTG
AAGAGCTACC
CTGTTCTTCT
CATACCTCGC
TTACCGGGTT
GGGGTTCGTG
AGCGTGAGCT
TAAGCGGCAG
ATCTTTATAG
CGTCAGGGGG
CCTTTTGCTG
ACCGTATTAC
ATTAGTTCAT
TGGCTGACCG
AACGCCAATA
CTTGGCAGTA
TAAATGGCCC
GTACATCTAC
TGGGCGTGGA
TGGGAGTTTG
CCCATTGACG
TTTAGTGAAC
TTGCGGCCGC
CAAGCCCGAC
GATCGAGGGC
GAAGGAGGGA
CAACAGGGCC
CGAGGGCTAC
GTCCGACATC
CTTCCCCCCC
GAGGATGTAC
GGGCGGCGGC
GCTGCCCGAC
CAACAAGGTG
GCTGTACAAG
CACATTTGTA
ACATAAAATG
ATAAAGCAAT
TGGTTTGTCC
CGGGTGTGGT
CTTTCGCTTT
ATCGGGGGCT
TTGATTAGGG
TGACGTTGGA
ACCCTATCTC
TAAAAAATGA
CAATTTAGGT
AATACATTCA
TTGAAAAAGG
GGCATTTTGC
AGATCAGTTG
TGAGAGTTTT
TGGCGCGGTA
TTCTCAGAAT
GACAGTAAGA
ACTTCTGACA
TCATGTAACT
GCGTGACACC
ACTACTTACT
AGGACCACTT
CGGTGAGCGT
TATCGTAGTT
CGCTGAGATA
TATACTTTAG
TTTTGATAAT
CCCCGTAGAA
CTTGCAAACA
AACTCTTTTT
AGTGTAGCCG
TCTGCTAATC
GGACTCAAGA
CACACAGCCC
ATGAGAAAGC
GGTCGGAACA
TCCTGTCGGG
GCGGAGCCTA
GCCTTTTGCT
CGCCATGCAT
AGCCCATATA
CCCAACGACC
GGGACTTTCC
CATCAAGTGT
GCCTGGCATT
GTATTAGTCA
TAGCGGTTTG
TTTTGGCACC
CAAATGGGCG
CGTCAGATCC
CTCGAGGCCA
ATGAAGATCA
GAGGGCGAGG
GCCCCCCTGC
TTCACCAAGT
TCTTGGGAGC
TCCATGGAGG
AACGGCCCCG
GTGCGCGACG
CACCACCGCG
TATCACTTTG
ACCGTTTACG
TAAGCATGCA
GAGGTTTTAC
AATGCAATTG
AGCATCACAA
AAACTCATCA
GGTTACGCGC
CTTCCCTTCC
CCCTTTAGGG
TGATGGTTCA
GTCCACGTTC
GGTCTATTCT
GCTGATTTAA
GGCACTTTTC
AATATGTATC
AAGAGTATGA
CTTCCTGTTT
GGTGCACGAG
CGCCCCGAAG
TTATCCCGTA
GACTTGGTTG
GAATTATGCA
ACGATCGGAG
CGCCTTGATC
ACGATGCCTG
CTAGCTTCCC
CTGCGCTCGG
GGGTCTCGCG
ATCTACACGA
GGTGCCTCAC
ATTGATTTAA
CTCATGACCA
AAGATCAAAG
AAAAAACCAC
CCGAAGGTAA
TAGTTAGGCC
CTGTTACCAG
CGATAGTTAC
AGCTTGGAGC
GCCACGCTTC
GGAGAGCGCA
TTTCGCCACC
TGGAAAAACG
CACATGTTCT
TGGAGTTCCG
CCCGCCCATT
ATTGACGTCA
ATCATATGCC
ATGCCCAGTA
TCGCTATTAC
ACTCACGGGG
AAAATCAACG
GTAGGCGTGT
GCTAGCTCTA
CCATGGTGAG
AGCTGAAGAT
GCAAGCCCTA
CCTTCTCCTA
ACCCCGACGA
GCACCATGAC
AGGACTCCTT
TGATGCAGAA
GCGTGCTGAA
TTGACTTCAA
TGGACCACCG
AGATCGCCGT
GATCCACCGG
TTGCTTTAAA
TTGTTGTTAA
ATTTCACAAA
ATGTATCTTA
AGCGTGACCG
TTTCTCGCCA
TTCCGATTTA
CGTAGTGGGC
TTTAATAGTG
TTTGATTTAT
CAAAAATTTA
GGGGAAATGT
CGCTCATGAG
GTATTCAACA
TTGCTCACCC
TGGGTTACAT
AACGTTTTCC
TTGACGCCGG
AGTACTCACC
GTGCTGCCAT
GACCGAAGGA
GTTGGGAACC
TAGCAATGGC
GGCAACAATT
CCCTTCCGGC
GTATCATTGC
CGGGGAGTCA
TGATTAAGCA
AACTTCATTT
AAATCCCTTA
GATCTTCTTG
CGCTACCAGC
CTGGCTTCAG
ACCACTTCAA
TGGCTGCTGC
CGGATAAGGC
GAACGACCTA
CCGAAGGGAG
CGAGGGAGCT
TCTGACTTGA
CCAGCAACGC
TTCCTGCGTT
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc.
Enzyme
AatII
Acc113I
Acc16I
Acc65I
AccB1I
AccB7I
AccBSI
AcsI
AcyI
AflIII
AhdI
Alw21I
Alw44I
AlwNI
Ama87I
ApaLI
ApoI
AseI
AsnI
Asp700I
Asp718I
AspEI
AspHI
AvaI
AviII
BalI
BamHI
BanI
BanII
BbiII
BbuI
Bbv12I
BcgI
BclI
BcoI
BglI
BpmI
BsaAI
BsaBI
BsaHI
BsaI
BsaMI
BsaOI
BsaWI
Bse118I
Bse8I
BseRI
Bsh1285I
Bsh1365I
BshNI
BsiEI
BsiHKAI
BsiI
BsmI
BsoBI
Bsp1407I
Bsp143II
Bsp19I
BspCI
BspHI
BspLU11I
BsrBI
BsrBRI
BsrDI
BsrFI
BsrGI
BssAI
BssSI
BssT1I
BstD102I
BstDSI
BstEII
BstH2I
BstI
BstMCI
BstPI
BstSFI
BstSNI
BstX2I
Cuts
5
1
1
1
5
1
2
4
7
2
1
3
2
1
2
2
4
2
2
1
1
1
3
2
1
1
1
5
2
7
1
3
1
1
2
5
3
3
1
7
3
2
4
4
3
1
2
4
1
5
4
3
2
2
2
1
3
3
1
2
1
2
1
2
3
1
3
2
3
2
4
1
3
1
4
1
5
1
11
BstXI
BstYI
1
11
BstZI
CciNI
Cfr10I
CfrI
DraI
DraII
DraIII
DrdI
DsaI
1
1
3
4
4
1
2
2
4
Positions
125 178 261 447 1149
2513
2771
619
465 619 785 1810 3041
1092
1710 2155
613 1549 2034 2045
122 175 258 444 1098 1146 2454
1621 3882
2994
2326 2411 3572
2322 3568
3473
641 929
2322 3568
613 1549 2034 2045
7 2819
7 2819
2394
619
2994
2326 2411 3572
641 929
2771
738
605
465 619 785 1810 3041
823 1780
122 175 258 444 1098 1146 2454
1368
2326 2411 3572
2478
1392
641 929
90 212 283 1220 2876
808 1171 2909
340 1120 1851
1397
122 175 258 444 1098 1146 2454
676 1087 2927
1485 1584
637 2476 2625 3548
1376 2698 3529 3676
1088 1746 2909
1397
673 1012
637 2476 2625 3548
1397
465 619 785 1810 3041
637 2476 2625 3548
2326 2411 3572
2330 3714
1485 1584
641 929
1353
1696 1704 3642
360 651 1002
2625
2154 3162
3882
1710 2155
1397
2758 2940
1088 1746 2909
1353
1088 1746 2909
2330 3714
360 651 1002
1710 2155
360 651 1002 1317
1297
1696 1704 3642
605
637 2476 2625 3548
1297
1207 1628 2748 3426 3617
340
585 605 1266 1370 1379 2347 2364
3132 3144 3230 3241
802
585 605 1266 1370 1379 2347 2364
3132 3144 3230 3241
634
634
1088 1746 2909
634 736 1177 2601
1437 2416 3108 3127
876
1249 1854
1898 3780
360 651 1002 1317
Sequence
gacgt/c
agt/act
tgc/gca
g/gtacc
g/gyrcc
ccannnn/ntgg
gagcgg
r/aatty
gr/cgyc
a/crygt
gacnnn/nngtc
gwgcw/c
g/tgcac
cagnnn/ctg
c/ycgrg
g/tgcac
r/aatty
at/taat
at/taat
gaann/nnttc
g/gtacc
gacnnn/nngtc
gwgcw/c
c/ycgrg
tgc/gca
tgg/cca
g/gatcc
g/gyrcc
grgcy/c
gr/cgyc
gcatg/c
gwgcw/c
cgannnnnntgc
t/gatca
c/ycgrg
gccnnnn/nggc
ctggag
yac/gtr
gatnn/nnatc
gr/cgyc
ggtctc
gaatgc
cgry/cg
w/ccggw
r/ccggy
gatnn/nnatc
gaggag
cgry/cg
gatnn/nnatc
g/gyrcc
cgry/cg
gwgcw/c
ctcgtg
gaatgc
c/ycgrg
t/gtaca
rgcgc/y
c/catgg
cgat/cg
t/catga
a/catgt
gagcgg
gatnn/nnatc
gcaatg
r/ccggy
t/gtaca
r/ccggy
ctcgtg
c/cwwgg
gagcgg
c/crygg
g/gtnacc
rgcgc/y
g/gatcc
cgry/cg
g/gtnacc
c/tryag
tac/gta
r/gatcy
ccannnnn/ntgg
r/gatcy
c/ggccg
gc/ggccgc
r/ccggy
y/ggccr
ttt/aaa
rg/gnccy
cacnnn/gtg
gacnnnn/nngtc
c/crygg
EaeI
EagI
Eam1104I
Eam1105I
EarI
EclHKI
EclXI
Eco105I
Eco130I
Eco24I
Eco255I
Eco31I
Eco52I
Eco57I
Eco64I
Eco88I
Eco91I
EcoO109I
EcoO65I
EcoRI
EcoT14I
EcoT22I
ErhI
Esp1396I
FauNDI
FbaI
FriOI
FspI
GsuI
HaeII
Hin1I
HincII
HindII
HindIII
HpaI
Hsp92I
KpnI
Ksp22I
Ksp632I
MamI
MfeI
MflI
4
1
1
1
1
1
1
1
3
2
1
3
1
4
5
2
1
1
1
1
3
1
3
1
1
1
2
1
3
3
7
2
2
1
1
7
1
1
1
1
1
11
MluI
MluNI
Mph1103I
MroNI
MscI
MslI
Msp17I
MspA1I
MunI
Mva1269I
NaeI
NcoI
NdeI
NgoAIV
NgoMI
NheI
NotI
NsiI
NspBII
NspI
PaeI
PaeR7I
PflMI
Ple19I
Ppu10I
PshBI
Psp1406I
PspEI
PstNHI
PvuI
RcaI
ScaI
SfcI
Sfr274I
SnaBI
SphI
SspBI
SspI
StyI
Van91I
VneI
VspI
XbaI
XcmI
XhoI
XhoII
1
1
1
1
1
6
7
3
1
2
1
3
1
1
1
1
1
1
3
2
1
1
1
1
1
2
2
1
1
1
2
1
5
1
1
1
1
2
3
1
2
2
2
1
1
11
XmaIII
XmnI
Zsp2I
1
1
1
allelebiotech.com
634 736 1177 2601
634
2205
2994
2205
2994
634
340
360 651 1002
823 1780
2513
676 1087 2927
634
718 1141 2312 3360
465 619 785 1810 3041
641 929
1297
876
1297
613
360 651 1002
3939
360 651 1002
1092
234
1392
823 1780
2771
808 1171 2909
1696 1704 3642
122 175 258 444 1098 1146 2454
1192 1498
1192 1498
627
1498
122 175 258 444 1098 1146 2454
623
1392
2205
1397
1485
585 605 1266 1370 1379 2347 2364
3132 3144 3230 3241
1621
738
3939
1746
738
365 653 1001 2223 2582 2741
122 175 258 444 1098 1146 2454
2358 3299 3544
1485
1485 1584
1748
360 651 1002
234
1746
1746
591
634
3939
2358 3299 3544
1368 3886
1368
641
1092
2625
3935
7 2819
2392 2765
1297
591
2625
2154 3162
2513
1207 1628 2748 3426 3617
641
340
1368
1353
2059 2189
360 651 1002
1092
2322 3568
7 2819
597 1382
1339
641
585 605 1266 1370 1379 2347 2364
3132 3144 3230 3241
634
2394
3939
y/ggccr
c/ggccg
ctcttc
gacnnn/nngtc
ctcttc
gacnnn/nngtc
c/ggccg
tac/gta
c/cwwgg
grgcy/c
agt/act
ggtctc
c/ggccg
ctgaag
g/gyrcc
c/ycgrg
g/gtnacc
rg/gnccy
g/gtnacc
g/aattc
c/cwwgg
atgca/t
c/cwwgg
ccannnn/ntgg
ca/tatg
t/gatca
grgcy/c
tgc/gca
ctggag
rgcgc/y
gr/cgyc
gty/rac
gty/rac
a/agctt
gtt/aac
gr/cgyc
ggtac/c
t/gatca
ctcttc
gatnn/nnatc
c/aattg
r/gatcy
a/cgcgt
tgg/cca
atgca/t
g/ccggc
tgg/cca
caynn/nnrtg
gr/cgyc
cmg/ckg
c/aattg
gaatgc
gcc/ggc
c/catgg
ca/tatg
g/ccggc
g/ccggc
g/ctagc
gc/ggccgc
atgca/t
cmg/ckg
rcatg/y
gcatg/c
c/tcgag
ccannnn/ntgg
cgat/cg
a/tgcat
at/taat
aa/cgtt
g/gtnacc
g/ctagc
cgat/cg
t/catga
agt/act
c/tryag
c/tcgag
tac/gta
gcatg/c
t/gtaca
aat/att
c/cwwgg
ccannnn/ntgg
g/tgcac
at/taat
t/ctaga
ccannnnn/nnnntgg
c/tcgag
r/gatcy
c/ggccg
gaann/nnttc
atgca/t
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc.
allelebiotech.com
The following enzymes do not cut:
AatI, AccI, AccIII, AclNI, AfeI, AflII, AgeI, AocI, Aor51HI, ApaI, AscI, AspI, AtsI, AvrII, BanIII, BbeI, BbrPI, BbsI, Bbv16II, BfrI, BglII, BlnI, BlpI, BpiI, Bpu1102I, Bpu14I, BpuAI, Bsa29I, BscI, Bse21I, BseAI,
BseCI, BsePI, BsgI, BsiMI, BsiWI, BsmBI, Bsp106I, Bsp119I, Bsp120I, Bsp13I, Bsp1720I, Bsp68I, BspDI, BspEI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, BstBI, Bsu15I, Bsu36I, CelII, Cfr42I, Cfr9I,
ClaI, CpoI, Csp45I, CspI, CvnI, Ecl136II, Eco147I, Eco32I, Eco47III, Eco72I, Eco81I, EcoICRI, EcoNI, EcoRV, EheI, Esp3I, FseI, KasI, Kpn2I, KspI, LspI, MroI, MspCI, NarI, NruI, NspV, PacI, Pfl23II, PinAI,
PmaCI, Pme55I, PmeI, PmlI, PpuMI, PshAI, Psp124BI, Psp5II, PspAI, PspALI, PspLI, PspOMI, PstI, PvuII, RsrII, SacI, SacII, SalI, SapI, SbfI, SexAI, SfiI, Sfr303I, SfuI, SgfI, SgrAI, SmaI, SmiI, SpeI, SplI, SrfI,
Sse8387I, SseBI, SstI, SstII, StuI, SunI, SwaI, Tth111I, Vha464I, XmaI
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc.
allelebiotech.com
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Current All ustious Fluorescent Protein Family Members:
The founding member is mTFP1.
mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a
slightly red-shifted emission spectrum and is 1.5 fold brighter compared to
EGFP. While being very bright, mTFPG3 can be photobleached within ~5
sec, about 30 times faster than EGFP, suitable for certain cell-based
assays that require a bright FP with very short half-life.
mTFP0.7 is a precursor during the evolution of mTFP1. It has
photo-switchable properties like Dronpa that cycles between fluorescent
and nonfluorescent states. It may be developed into components in
PALM/SIM applications.
Basic Vectors
Three vectors are available: pNCS-mWasabi, pmWasabi-N and
pmWasabi-C.
Subcellular Marker Vectors
Twenty six vectors are available.
Vectors in Viral Vectors
All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter
Lentiviral Vectors.
IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government
research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such
license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party.
Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more
information please visit Fluorescent Protein Sublicense, or contact us via phone or email.
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645
Fax: (858) 587-6692 [email protected] Copyright ©2008-2010