5. Lab Safety Using the Microscope

Lab Safety
Rules:
No Running
No eating, smelling or drinking your lab
No horseplay or fooling around
Handle glassware and equipment with care
Be careful around fires and flames
If you make a mess clean it up
Data - The numbers or values you find during the
lab.
Results - A sentence (or more) explaining what you
saw happen during the lab.
Conclusion - A statement about what your data and
results mean. A statement about your hypothesis
(are you right or wrong). Answers to the questions
in the "Analysis" section of the lab in the textbook.
Sources of Error - What things went wrong? What
may have happened that may have had an effect on
your lab (things you may not be able to control as
well as those you can). What impact did these
things have on the lab?
Purpose - Why are we doing the lab? What are we trying
to learn?
Hypothesis - What do you think is going to happen? Why
do you think this? State specifically what information you
are basing this on and where you learned/heard/saw this
information.
Materials - List everything you use including size and
amount.
Procedure - Write the step-by-step directions for doing
the lab.
Poster Assignment
Prepare a poster that groups pictures into three
categories:
1. Things that are living
2. Things that were once living
3. Things that are not living and never have been living
For example: A steak is not currently living but it does
contain muscle cells, because the cow was once living.
Plastic comes from ethylene molecules that were never
living.
Dutch
1600
Microscope
What is a Microscope?
A microscope (Greek: micron = small and scopos = aim)
is an instrument for viewing objects that are too
small to be seen by the naked or unaided eye.
Ten parts to the microscope
2. What are the parts of a microscope?
Eye piece
Focus
Fine Focus
Objective Lenses
Sample on slide
Condenser
Stage
Diaphragm
Light source
Base
3. What is the function of each part of the microscope
Eye piece: the lens at the top that you look through.
They are
usually 10X or 15X power.
Focus:
large, round knob on the side of the microscope used for
focusing the specimen; it may move either the stage or the upper
part of the microscope.
Fine adjustment knob: small, round knob on the side of the
microscope used to fine-tune the focus of your specimen after using
the coarse adjustment knob.
Objective lenses: (low, medium, high, oil immersion) the microscope
may have 2, 3 or more objectives attached to the nosepiece; they
vary in length (the shortest is the lowest power or magnification; the
longest is the highest power or magnification).
Stage: large, flat area under the objectives; it has a hole in it (see
aperture) that allows light through; the specimen/slide is placed on the
stage for viewing.
The purpose of the condenser lens is to focus the
light onto the specimen.
Diaphragm: controls the amount of light going through the
condenser.
Base: The bottom of the microscope, used for support
VIRTUAL
MICROSCOPE
LAB
Instructions: With the provided
worksheet connect to the given
website, answer the questions and
submit your answer to the
instructor.
Work sheet on microscopes
Go and practice your Microscope
knowledge
MICROSCOPE QUIZ
What is your field of view when using a
microscope?
The field of view is the circle of
light you see when you look through
the microscope.
5. How do the lenses in a microscope
work?
The lenses in the microscope help bend
the light source in a direction that we
are able to see the image on the slide in
the field of view.
The lens used in microscopes are convex
lenses.
6. How does light pass through the convex
lens?
Most lenses are made out of glass. Lenses will either
magnify/reduce images or concentrate/spread out light rays.
If the objective lens magnifies 4x and the ocular lens
Eyethe
piece
lens Magnification
magnifies 10x, then
viewerObjective
sees a magnified
image of 40X.
Explain.
Low Power
10x
10x
100x
High Power
10x
43x
430x
Oil lens
10x
97x
970x
Explain the above table
http://www.usoe.k12.ut.us/curr/science/sciber00/7
th/cells/sciber/micrpart.htm
What magnification gives you the best
image?
The best way to get a good
magnification of an object is to start
off with the low power. Refer to the
table in the previous slide.
The MOST common mistake a student
makes is starting on high power.
Microscope Crime Mystery
Write a mystery that involves a crime that can
be solved by using a microscope.
Set the scene for the mystery, profile the
suspects and provide clearly labeled evidence
in clear sealed bags (glad).
You can use things such as finger prints, carpet
or clothing samples, hair samples or soil
samples as evidence.
Work in groups of three-four.
Story must be 5 pages in length, double
spaced, typed with a cover page.
10-story, 10-evidence, 5-participation