Title
Evaluation of nickel tolerance in Amaranthus paniculatus L. plants by
measuring photosynthesis, oxidative status, antioxidative response and
metal-binding molecule content
Author Name
Fabrizio Pietrini, Valentina Iori, Alexandra Cheremisina, Nina I. Shevyakova,
Nataliya Radyukina, Vladimir V. Kuznetsov, Massimo Zacchini
Journal Name
Year
Volume and
Issuue
Pages
Abstracts
Environmental Science and Pollution Research
2015
22, Issue 1
482-494
Among metals, Ni has been indicated as one of the most dangerous for the
environment, and plants exposed to this metal are frequently reported to
undergo a severe stress condition. In this work, the tolerance responses to
different Ni concentrations at physiological and biochemical levels were
evaluated in Amaranthus paniculatus L., a plant species previously
characterised for their ability to phytoremove Ni from metal-spiked water.
Results indicated a good metal tolerance of this plant species at
environmentally relevant Ni concentrations, while clear symptoms of
oxidative damages were detected at higher Ni concentrations, both in roots
and leaves, by measuring lipid peroxide content. At the photosynthetic level,
pigment content determination, chlorophyll fluorescence image analysis and
gas-exchange parameter measurements revealed a progressive impairment of
the photosynthetic machinery at increasing Ni concentrations in the solution.
Regarding biochemical mechanisms involved in antioxidative defence and
metal binding, antioxidative enzyme (ascorbate peroxidase, APX; catalase,
CAT; guaiacol peroxidase, GPX; superoxide dismutase, SOD) activity,
polyamine (PA) content, polyamine oxidase (PAO) activity and organic acid
(OA) content were differently affected by Ni concentration in the growth
solution. A role for GPX, SOD, PAs, and oxalic and citric acid in Ni
detoxification is suggested. These results can contribute to elucidate the
tolerance mechanisms carried out by plants when facing environmentally
relevant Ni concentrations and to identify some traits characterising the
physiological and biochemical responses of Amaranthus plants to the
presence and bioaccumulation of Ni.
Keywords
Title
Photosynthetic, Amaranthus, bioaccumulation , chlorophyll fluorescence
Photosynthetic response and proline bioaccumulation in black gram
induced by Nickel stress
Author Name
Das S, Samantaray S, Mohanty RC, Mohanty M, Pradhan C
Journal Name
The International Daily journal
Year
Volume and
Issuue
Pages
Abstracts
2015
27 (97),
36-42
The present study assess the impact of varying treatment concentrations of
nickel (Ni) on photosynthetic responses and stress induced praline
accumulation in a leguminous plant i.e. black gram [Vigna mungo (L.)
Hepper] cultivars (PU31C and Shekhar1C). After exposure to different
concentrations of toxic Ni2+, the two cultivars of V. mungo i.e. PU31C and
Shekhar1C showed significant changes in their photosynthetic
response. Sekhar1C variety showed high chlorophyll content in seedlings
treated with toxic Ni (200μM) when compared to Control and other variety.
PU31C variety showed four times more Chlorophyll content than seedlings of
control treatment. The present hydroponic study exhibited stimulatory effects
of nickel on total chlorophyll content, carotenoid and increased proline level
after two weeks exposure period. Present preliminary study indicates the
tolerance nature of two green gram cultivars to toxic doses of Ni+2.
Keywords
Nickel, Chlorophyll, Proline, Hydroponics
Title
Estimation of plant growth promoting potential of a nickel accumulating
isolate obtained from dhapa industrial wasteland (Kolkata, India) soil on
Indian yellow mustard (Brassica hirta)
Author Name
Santanu Maitra and Pranab Kumar Banerjee
Journal Name
Int.J.Curr.Microbiol.App.Sci
Year
2015
Volume and
4 (1)
Issuue
Pages
Abstracts
765-772
Plant growth promoting bacteria (PGPB) are known to influence plant growth
by various direct or indirect mechanisms. Present study was conducted with
an aim to estimate the PGP potential of one nickel tolerant bacterial isolate
from Dhapa industrial wasteland, Kolkata, India. Isolate I (Gram negative
coccobacilli) was observed to tolerate and accumulate significant amounts of
nickel and also have multiple Plant Growth Promoting (PGP) activities like
IAA production and phosphate solubilization. Present study also shows that
seeds of yellow mustard (Brassica hirta) inoculated with the test isolate
individually, significantly enhanced root and shoot growth and also protected
the plant from the various phytotoxic effects of nickel.
Keywords
Nickel;, accumulation; IAA production; Phosphate solubilization; Brassica
hirta Bioremediation
Title
Root based responses account for Psidium guajava survival at high nickel
concentration
Author Name
Nadia Bazihizinaa, Mirvat Redwana, Cosimo Taitia, Cristiana Giordanob,
Emanuela Monettia, Elisa Masia, Elisa Azzarelloa, Stefano Mancuso
Journal Name
Journal of plant physiology
Year
Volume and
2015
174 (10
Issuue
Pages
Abstracts
137-146
The presence of Psidium guajava in polluted environments has been reported
in recent studies, suggesting that this species has a high tolerance to the metal
stress. The present study aims at a physiological characterization of P.
guajava response to high nickel (Ni) concentrations in the root-zone. Three
hydroponic experiments were carried out to characterize the effects of toxic
Ni concentrations on morphological and physiological parameters of P.
guajava, focusing on Ni-induced damages at the root-level and root ion
fluxes. With up to 300 μM NiSO4 in the root-zone, plant growth was similar
to that in control plants, whereas at concentrations higher than 1000 μM
NiSO4 there was a progressive decline in plant growth and leaf gas exchange
parameters; this occurred despite, at all considered concentrations, plants
limited Ni2+ translocation to the shoot, therefore avoiding shoot Ni2+
toxicity symptoms. Maintenance of plant growth with 300 μM Ni2+ was
associated with the ability to retain K+ in the roots meanwhile 1000 and 3000
μM NiSO4 led to substantial K+ losses. In this study, root responses mirror
all plant performances suggesting a direct link between root functionality and
Ni2+ tolerance mechanisms and plant survival. Considering that Ni was
mainly accumulated in the root system, the potential use of P. guajava for
Ni2+ phytoextraction in metal-polluted soils is limited; nevertheless, the
observed physiological changes indicate a good Ni2+ tolerance up to 300 μM
NiSO4 suggesting a potential role for the phytostabilization of polluted soils.
Keywords
Ion fluxes; Phytostabilization; Impedance spectroscopy; Gas exchange;
Heavy metals
Title
Modelling uptake and toxicity of nickel in solution to Enchytraeus
crypticus with biotic ligand model theory
Author Name
Erkai He, Hao Qiu, Cornelis A.M. Van Gestel
Journal Name
Environmental pollution
Year
2014
Volume and
Issuue
Pages
188
Abstracts
17–26
Protons and other cations may inhibit metal uptake and alleviate metal
toxicity in aquatic organisms, but less is known about these interactions in
soil organisms. The present study investigated the influence of solution
chemistry on uptake and toxicity of Ni in Enchytraeus crypticus after 14 days
exposure. Ca2+, Mg2+ and Na+ were found to exert significant effects on both
uptake and toxicity of Ni. An extended Langmuir model, which incorporated
cation competition effects, well predicted Ni uptake. The LC50{Ni2+}
predicted by a developed Biotic Ligand Model matched well with observed
values. These suggest that cation competition needs to be taken into account
when modelling uptake and effects. The binding constants of Ni2+, Mg2+ and
Na+ on the uptake and toxic action sites were similar, but for Ca2+ they
differed. This indicates that the effect of Ca2+ on Ni2+ toxicity cannot simply
be explained by the competition for entry into organism.
Keywords
Title
Nickel, Uptake, Toxicity, Langmuir model, Biotic Ligand Model
Characterisation of the wheat phospholipid fraction in the presence of
nickel and/or selenium
Author Name
Przemysław Bernat, Ewa Gajewska, Tadeusz Bernat, Marzena Wielanek
Journal Name
Plant Growth Regulation
Year
Volume and
Issuue
2014
72, Issue 2
Pages
Abstracts
163-170
The influence of nickel (Ni) and/or selenium (Se) on phospholipid
composition was studied in shoots and roots of wheat seedlings.
Phospholipid differences between samples were analysed using liquid
chromatography/electrospray ionization–MS coupled. A total of 39 lipid
species were identified. Individual phospholipids were then quantified
using a multiple reaction monitoring method. In the roots, Ni toxicity was
associated with an elevated level of phosphatidic acid species. In the shoots,
the phosphatidylcholine/phosphatidylethanolamine ratio was about fivefold
higher than in roots and decreased in Ni-treated samples. Additionally, the
concentrations of phospholipid species containing C 18:3 fatty acid were
reduced. Lipidome data were then analyzed using principal component
analysis, which confirmed the compositional changes in phospholipids in
response to Ni and Ni + Se. In contrast, the phospholipid profiles of wheat
seedlings exposed to Se alone showed more similarities with the control.
Together, our results suggested that the presence of Se, despite a
considerable improvement of growth of Ni-treated wheat, did not
counterbalance negative effect of Ni on the phospholipid composition in
wheat roots and shoots.
Keywords
Title
Ni, Phospholipid, chromatography, Lipidome, electrospray ionization
Nickel and zinc effects, accumulation and distribution in ruderal plants
Lepidium ruderale and Capsella bursa-pastoris
Author Name
Anna D. Kozhevnikova, Nadezhda T. Erlikh, Natalia V. Zhukovskaya,
Natalie V. Obroucheva, Victor B. Ivanov, Anastasiya A. Belinskaya,
Milena Y. Khutoryanskaya, Ilya V. Seregin
Journal Name
Year
Volume and
Issuue
Acta Physiologiae Plantarum
2014
36, Issue 12
Pages
Abstracts
3291-3305
Ruderal plants can grow in polluted areas, but little is known about heavy
metal accumulation and distribution in them. Here Ni and Zn accumulation,
distribution and effects were investigated in Lepidium ruderale and
Capsella bursa-pastoris grown at 5–30 µM Ni(NO3)2 or 10–80 µM
Zn(NO3)2. Metal contents were measured by flame atomic absorption
spectrophotometry and tissue distribution of metals was studied
histochemically. Ni was more toxic than Zn for both plants. When metalinduced growth-inhibiting effects were compared at various metal
concentrations in solution, L. ruderale was more tolerant to Ni, whereas C.
bursa-pastoris to Zn. However, when compared at similar Zn or Ni
contents in roots, root growth of C. bursa-pastoris was more tolerant than
that of L. ruderale. On the contrary, at similar Zn or Ni contents in shoots,
shoot growth of L. ruderale was more tolerant. Both plants are excluders
maintaining low metal levels in shoots. In roots, Ni located in protoplasts
while Zn was also detected in cell walls. Metal accumulation in root apices
resulted in growth inhibition. Ni accumulation in root cortex constrained
metal translocation into central cylinder and then to shoots, where it located
only in conductive tissues and epidermis, particularly in leaf trichomes of
C. bursa-pastoris. Zn was translocated to shoots more actively and
distributed in all shoot tissues, being accumulated in leaf vascular bundles
and epidermis. To conclude, these patterns of Ni and Zn distribution are
aimed at metal sequestration in roots and leaf epidermis, thus keeping
mesophyll from metal penetration and pigment degradation.
Keywords
Ni and Zn, Lepidium ruderale, Metal accumulation, pigment degradation