CLS Product Information: MG-60
Ham’s F12
Order Number: 820600
500 ml
Concentration
Ready-to-use
mg/L
Inorganic salts
mg/L
Vitamins
CaCl2 (anhydrous)
KCl
33.30
223.60
Mg SO4 (anhydrous)
72.20
NaCl
7600
FeSO4· 7H2O
0.834
NaHCO3
1176.00
Na2HPO4 (anhydrous)
142.00
CuSO4 (anhydrous)
0.0016
ZnSO4· 7H2O
0.863
Choline chloride
13.96
Folic acid
1.300
i-Inositol
Vitamin B1 · HCl (Thiamine
Monohydrochloride)
Vitamin B2 (Riboflavin)
18.02
0.340
0.038
Vitamin B5 (D-Calcium Pantothenate)
Vitamin B6 · HCl (Pyridoxine
Monohydrochloride)
Nicotinamide
0.480
Vitamin B12 (Cyanocobalamin)
1.360
0.062
0.037
Vitamin H (D-Biotin)
0.0073
Amino acids
L-Alanine
8.9
L-Arginine · HCl
211.00
Other components
D(+)-Glucose
L-Asparagine · H2O
15.00
DL-Thioctic acid
L-Aspartic acid
13.30
Phenol Red, Na
L-glutamic acid
14.70
Na-pyruvate
Glycine
7.50
1802.00
0.210
1.200
110.00
Putrescine · 2HCl
0.16
L-Histidine · HCl · H2O
20.96
Thymidine
0.73
L-Cysteine· HCl· H2O
35.12
Hypoxanthine, Na
4.77
L-Leucine
13.10
Methyl lineoleate
0.088
L-Isoleucine
3.94
L-Lysine · HCl
36.50
Specifications
L-Methionine
4.48
Final pH
7.2 ± 0.2
L-Phenylalanine
4.96
Osmolality (mOsm/kg)
290 ± 30
L-Proline
34.50
L-Serine
10.50
L-Threonine
11.90
Fetal Bovine Serum
L-Tryptophane
2.04
Note:
L-Tyrosine · 2Na · 2H2O
7.84
In case FBS was added, the concentrations
L-Valine
11.70
L-Glutamine
10%
given above need to be reduced by 10%.
146.20
Maintenance
Immediately after delivery, store the medium at 4°C to 8° C in the dark. Do not heat the medium over 37°C or use
uncontrollable sources of heat (e.g. microwave appliances). If only a part of the medium is to be used, remove this
amount from the bottle and warm it up. Do not freeze the medium. This can result in high salt concentrations by
freezing out pure water which will cause irreversible damage.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.
CLS Cell Lines Service GmbH - Dr. Eckener-Straße 8 - 69214 Eppelheim - Germany
Tel: +49(0)6221 700799
FAX +49(0)6221 700717
[email protected] www.clsgmbh.de
Page 1 of 1