15
th
ALLCELLS
®
Anniversary
Est. 1998
Product Catalog 2013-2014
Your Reliable Source of Primary Cells and Bioservices
Healthy Cells
IRB Approved
Tissue Collection
Facility
Bioservices
Diseased Cells
1
ALLCELLS
What makes our cells different?
QUALITY
ALLCELLS ADVANTAGE
FLEXIBILITY
•  Large healthy donor repository
EXPERTISE
•  Diseased tissue and cells network
•  Own IRB approved tissue collection
facility
•  Availability of over 200 cell types from
various tissues
•  Custom products/bioservices
•  Easy inventory access
SUPPLY
CHAIN
CONTROL
•  Worldwide delivery
SCALABILITY
•  15 years experience
RELIABILITY
AllCells, LLC
1301 Harbor Bay Park way, Suite 200
Alameda, California 94502
Phone: 1-888-535-3444, 510 -521-2600
AllCells,
LLC
Toll Free
1-888-535-3444
Toll
FreePhone:
Phone:
1-888-535-3444
Fa x: 510 -521-7600
Email: [email protected]
ALLCELLS
09-2013
w w w.allcells.com
Cover Image:
Highly magnified view of human blood during a leukemoid reaction, transient myeloproliferative disorder.
Diseased Cells Image:
www.allcells.com For
For In
Research
Use Use
Only Only
www.allcells.com
InVitro
Vitro
Research
This image was originally published in ASH Image Bank.
Peter Maslak. AML with t(3;3)-peripheral blood - 1. ASH Image Bank. 2011; 2011-3514.
The American Society of Hematology.
2
A Message to Our Valued Customers:
Dear AllCells Customer,
In our ongoing effort to keep you informed about the newest developments taking place at AllCells it is
my privilege to provide you with our new 2013-2014 Product Catalog. In addition to new products and
services, you will notice a fresh design that includes a more intuitive product order, enhanced index, and
tips page about how to best find the information you need. To improve your ability to glean online information quickly, more search filters have also been incorporated into www.AllCells.com.
In response to increasing demand in the field of autoimmune disease research we introduced in 2012
an expanded Diseased Product line including peripheral blood (PB) and isolated mononuclear cells
types from patients diagnosed with autoimmune diseases such as Rheumatoid Arthritis, Osteoarthritis,
Systemic Lupus Erythematosus (SLE), Psoriasis, Types I & II Diabetes, and Multiple Sclerosis. Through
an established and growing network of diseased tissue providers, AllCells continues to enhance its capability to source specialty hematologic diseased tissue for your specific research projects.
For research projects involving the use of Leuko Paks, in addition to our Fresh Normal PB and Mobilized
PB Leuko Pak product line, we introduced Fresh Normal PB Dual Half Paks (first & second half) to enable access to tissue from the same donor 6-8 weeks apart, thereby helping minimize assay variability.
At AllCells we truly appreciate the challenges life science researchers face with sourcing biological
components of cell-based assays. We know the success of your research can depend heavily upon obtaining a reliable supply of high quality hematopoietic tissue and/or primary cell products with appropriate cellularity, donor phenotype, and cell viability. That is why AllCells has committed its efforts towards
developing and continually improving our clinical tissue collection operations in a way that provides for
the timely flow of hematopoietic tissue through all steps of production, quality assurance, and product
packaging. This distinctive capability allows us to work closely with our customers to deliver the most
relevant tissue and purified cell types with unsurpassed quality.
To further support your research needs we continue to add many capabilities within the Bioservices
area of our business for customers who are looking for expertise with cell screening, characterization,
cell expansion/differentiation, functional assays, and/or developing other customized cell-based assays.
Please contact us at 1-888-535-3444 to learn more about how AllCells can add value to your specific
application.
On behalf of the AllCells team we look forward to having the opportunity to serve you.
Sincerely,
Jay Tong
Jay Tong, M.D.
President & CEO
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
3
Table of Contents
A Message to Our Customers
2
Helpful Information
Dendritic Cells (BDCA4+ Lymphoid/Plasmacytoid)
Natural Killer Cells (CD56+)52
51
Myeloid Cells
Basophils53
Using the Catalog5
Ordering/Delivery Information6-8
Getting Support9
General Product Information10-12
Terms & Conditions
13-14
Eosinophils54
Neutrophils55
Red Blood Cells/Mature Erythrocytes 56
Platelets 57
Monocytes
CD14+ Monocytes58
Tissue Collection Facility
Background Information15-16
Mobilization, Donor Criteria, Capabilities
17
Lead Times, Matched Sample Collection
18
Dendritic Cells (Monocyte-Derived)
59
Macrophages60
Mobilized Peripheral Blood
Bioservices
Background Information61
Background Information19-20
Mononuclear Cells
Immunoassays21
MNC63
Hematopoietic Assays22
CD34-Depleted MNC64
Flow Cytometry Services23
Stem/Progenitor Cells
Tissue Culture Services24
CD34+65
Protein Analysis25
CD133+ 66
Genotyping26
Myeloid Cells
Custom HLA-Typing27
Leuko Pak (Apheresis Collection)
62
Monocytes (CD14+)67
Lymphoid Cells
Human Primary Cells & Tissue
Background Information28-29
Custom Products/Projects30-32
New Products33
Natural Killer Cells (CD56+)68
Bone Marrow
Background Information69
Aspirates—Bone Marrow (Whole)
Normal Peripheral Blood
70
Mononuclear Cells (MNC) MNC 71
Background Information35
CD34-Depleted MNC72
Leuko Pak (Apheresis Collection)
Stem/Progenitor Cells
36
Whole Blood37
CD34 + 73
Plasma38
CD133 +74
Mononuclear Cells (MNC)39
Myeloid Cells Stem/Progenitor Cells (CD34+)40
Lymphoid Cells
CD33 76
Early Erythroid Progenitor Cells (CD36 +) 75
+
B Cells(CD19+, CD19+/IgD+, Memory)
41-43
T Cells (CD3+ Pan, CD4+ Helper, CD4+/CD25+,44-50
CD4+/CD45RA+/CD25- Naive Helper,
Mesenchymal Cells
Endothelial Cells (CD105 +) 77
Mesenchymal Stem Cells
78
CD4+/CD45RO+ Memory, CD8+ Cytotoxic,
CD8+/CD45RA+ Naive Cytotoxic)
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
4
Cord Blood
Multiple Myeloma (MM)137-142
Background Information79
Autoimmune
Plasma80
Background Information143
Mononuclear Cells (MNC) Lupus (SLE)144
MNC81
CD34-Depleted MNC82
Osteoarthritis (OA)146
CD133-Depleted MNC83
Psoriasis (PSO)147
Stem/Progenitor Cells
Rheumatoid Arthritis (RA)
148
CD34 +84
Type I Diabetes (DIA1)
149
CD133 85
Type II Diabetes (DIA2)
150
Myeloid Cells
Sickle Cell Anemia (SCA)
151
+
Early Erythroid Progenitor Cells (CD36 )
+
86
Multiple Sclerosis (MS)
145
Inflammatory Bowel Disease
Neutrophils 87
Background Information152
Crohn's Disease153
Monocytes (CD14 )88
+
Lymphoid Cells
Ulcerative Colitis154
B Cells (CD19 )89
T Cells (CD3+ Pan, CD4+ Helper, CD8+ Cytotoxic, +
90-93
CD4 /CD45RA Naive)
+
Natural Killer Cells (CD56 +)94
Human Umbilical Vascular Endothelial Cells (HUVEC)
95
Background Information155-156
New Products157
Non-Human Primate Cells158
Mouse Cells159-163
Fetal Liver
Background Information96
Unprocessed Fetal Liver
Animal Primary Cells & Tissue
+
97
Mononuclear Cells (MNC)98
Stem/Progenitor Cells
Rat Cells164-171
Pig Cells172
Cell Culture & Cryopreservation Media
CD34 +99
Background Information173
CD133 100
Cell Culture & Cryopreservation Media
+
Early Erythroid Progenitor Cells (CD36 ) 101
Mesenchymal Stem Cells 102
+
174
Technical Notes
How to Count Fresh Cells
176
How to Thaw Frozen Cells
177
Background Information103
How to Freeze Cell Products
178-179
Adipose-derived Stem Cells104
How to Purify Bone Marrow MNC
180-181
Amniotic/Placental Cells105
How to Count Leuko Pak
182
Dental Pulp Cells106
How to Deplete Red Blood Cells
183
How to Passage & Subculture Cells
184-187
Non-Hematopoietic Stem Cells & Tissue
Diseased Cells & Tissue
Background Information107-108
Index
Custom Products109-110
Alphabetical Index188-190
New Products111
Catalog # Index191-196
Leukemia
Background Information112-113
Acute Lymphoid Leukemia (ALL)
114-119
Acute Myeloid Leukemia (AML)
120-124
Myelodysplastic Syndrome (MDS)
125
Chronic Lymphoid Leukemia (CLL)
126-131
Chronic Myeloid Leukemia (CML)
132-136
ALLCELLS
Toll Free Phone: 1-888-535-3444
Notes197-198
Quick Reference Guide199-200
www.allcells.com
For In Vitro Research Use Only
5
Using The Catalog
We are excited to share our new 2013-2014 Product Catalog with you. We have worked hard to ensure the
catalog is a good resource for you. Here are some tips on how to get the most benefit from our new catalog.
General Information:
The section on pages 6 - 14 features helpful information on ordering, delivery, lead times, technical support,
AllCells.com website, general product information, and terms and conditions. Use this information to become
more familiar with our ordering process and how best to reach us.
Service sections:
We have outlined some of our unique research capabilities in the Tissue Collection Facility, Bioservices, and
Custom Products/Projects sections.
Product sections:
Beginning on page 35 you will find our product information, categorized by species and tissue type. Use the
Table of Contents on page 3 to find the section you desire. Each section then has a background page to help
you find what you are looking for quickly. Look for the 'For more information visit: www...' guides to explore more
on our website.
Technical Notes:
At the back of our new catalog we feature updated protocols, 'how-to' technical guides for the most common
research techniques we utilize at AllCells. Follow these notes to help ensure the best results with our cell
products. For more information you may access videos and printed guides here: www.allcells.com/support/
how-to
Indices:
Our 2013-2014 Product Catalog now features two Indices for your convenience: by topic and by catalog #.
Located on pages 188 - 199.
Quick Reference Guide:
The last pages of the catalog contain commonly-used reference tables and diagrams as a resource for your
research.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
6
Ordering & Delivery Information
Ordering Information
Contact Information
Phone
(510) 521-2600
Toll-Free
(888) 535-3444
Fax
(510) 521-7600
Email
[email protected]
Online
allcells.com
Address
1301 Harbor Bay Parkway, Suite 200
Alameda, CA 94502
Office Hours
Monday through Friday
8:30am to 5:30pm (Pacific Time)
Order, price, and delivery date are confirmed after AllCells receives your purchase
order or credit card information. Please provide us with your email address so we
can notify you about the shipment status. All International orders must be paid in full
prior to shipment.
Lead Times*
Product Format
Fresh
Normal Peripheral Blood
Mobilized Peripheral Blood
Leuko Paks and
Leuko Paks and
Leuko Pak derivatives:
Leuko Pak derivatives:
2 weeks
4 weeks
Frozen
Bone Marrow
Cord Blood
Fetal Liver
2 weeks
2 weeks
N/A
1-2 business days
Lysate/cDNA/RNA
2 weeks
*Lead times apply to healthy donor products only. Diseased tissue and cells are limited by stock availability. Lead times may vary due to
the nature of the disease and sensitivity of patients. Custom product lead times may vary. See Custom Product section on pg. 30.
Delivery Information
Media Shipment & Delivery Information
Media
Package
Examples
Stimulatory Supplement
Packaged in dry ice at
shipment.
MSC Stimulatory Supplement
Basal Medium
Packaged with a cold gel pack
at shipment.
MSC Basal Medium
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
Courier
Packages are shipped using FedEx
Priority Overnight service with a
delivery commitment of 10:30am local
time. Other delivery services, including
same day delivery, are available upon
request.
For In Vitro Research Use Only
7
Ordering & Delivery Information
Cell Type
Fresh
Frozen*/Lysates
Whole tissue
Transferred to a conical tube containing
DPBS buffer. Shipped at room
temperature.
Lysates are packaged in dry ice at
shipment.
Plasma/
Platelets
Shipped at room temperature.
Snap Frozen. Packaged in dry ice at
shipment.
Whole Blood
RBC
Drawn into a blood bag,
syringe(s), or blood tube(s). Shipped at
room temperature.
Transferred to a 50mL conical tube
containing fresh RBCs and shipping
media (DPBS + 0.5% BSA). Packaged
with a cold gel pack.
Transferred to a 50mL conical tube
with shipping media (DPBS + 0.5%
BSA + 2mM EDTA). Packaged with a
cold gel pack.
Frozen MNC are stored in a solution
of 50% IMDM, 40% FBS, 10%
DMSO (0.2 µm filtered) in the vapor
phase of liquid nitrogen storage until
ready for shipment. Packaged in dry
ice at shipment. (Exception: BM-MNC
are stored in standard CryoStor
media.)
Purified Cells
Transferred to a small centrifuge tube
containing shipping media (IMDM +
0.5% BSA + 2mM EDTA). Packaged
with a cold gel pack.
Frozen cells are stored in a solution of
50% IMDM, 40% FBS, 10% DMSO
(0.2 µm filtered) in liquid nitrogen
storage until ready for shipment.
Packaged in dry ice at shipment.
Leuko Pak
One-liter blood bag containing
peripheral blood cell MNCs and
autologous plasma. Shipped at room
temperature. Two-day collections are
shipped out separately.
Cultured Cells
Cells are cultured and transported in
an OptiCell plate that has a two 50cm2
gas permeable, optically clear growth
membranes.**
Mononuclear
Cells (MNC)
Stored in a 500μL microtube with 20
to 100 μL DEPC water. Packaged in
dry ice at shipment.
cDNA
Aliquoted at a base concentration of
one PCR reaction/2μL DEPC-H2O.
Packaged in dry ice at shipment.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Packages are shipped using
FedEx Priority Overnight
service with a delivery
commitment of 10:30am local
time. Other delivery services,
including same day delivery,
are available upon request.
Frozen cells are stored in a solution of
50% IMDM, 40% FBS, 10% DMSO
(0.2 µm filtered) in liquid nitrogen
storage until ready for shipment.
Packaged in dry ice at shipment.
RNA
Courier
www.allcells.com
For In Vitro Research Use Only
8
Ordering & Delivery Information
What additional information will accompany my AllCells Products?
Cell product packages contain:
In addition, each purified cell product package contains:
A Certificate of Analysis
A FACS Analysis Report
The Certificate of Analysis lists the following:
• Catalog number
• Catalog description
• Donor information (as provided)
• Viral results (HIV, HBV, HCV)
The figure below is a sample FACS Analysis Report for our
CD34+ enriched progenitor cells from human mobilized
peripheral blood. The upper right panel shows the viability
of cells and the lower right panel displays the purity of cells.
The lower left panel represents the control. Samples were
collected on a BD FACSCaliburTM.
If applicable:
• Cell viability
• Cell purity
• Cell count
AllCells, LLC
Email: [email protected]
www.allcells.com
FACS Analysis Report
Counts
40 60
R1
20
M1
0
Side Scatter
400
600
Side scatter
800
80 100
1000
Product Name: MPB-CD34+ Cells
ID#: A3179
Catalog #: mPB009
Purity of cells (lower right panel): 98%
Viabililty of cells (upper right panel): 97%
10 1
10 2
PI
10 3
10 4
0
200
10 0
400
600
Forward Scatter
800
Marker
All
M1
1000
120
Counts
60
90
Side scatter
Counts
60
80
M1
10 1
Marker
All
M1
10 2
Control
10 3
10 4
Events % Gated
9690
100.00
7
0.07
0
20
30
40
M1
0
10 0
Events % Gated
10000
100.00
9690
96.90
150
200
100 120
0
10 0
10 1
10 2
CD34-PE
10 3
Marker
All
M1
Events % Gated
9690
100.00
9580
98.86
10 4
Page 1
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
9
Getting Support
Technical Support
How to reach us
At AllCells we are committed to providing the best
support possible for our customers. We are dedicated
to sharing our expertise in the hematology field to
compliment our industry-leading products.
Customer service is a way of life at AllCells and we
pride ourserlves on going 'above and beyond' to
answer any support questions you may have. If you
were not able to find an answer to your question on
our website please contact us directly.
You may find multiple resources on our website
including:
Technical notes, product 'How to' guides, BioTube technical videos:
www.allcells.com/support/how-to
Webinars, support presentations:
www.allcells.com/support/webinars
General product information, availability, sales
inquiries, ordering: [email protected]
Technical questions, Bioservices, custom project
inquiries: [email protected]
Existing orders inquiries: [email protected]
General AllCells information: [email protected]
Publications, research citing AllCells products and services:
www.allcells.com/publications
Facebook: www.facebook.com/allcells
LinkedIn: www.linkedin.com/company/allcells-llc
Product Data Sheets, request Certificate of
Analysis, MSDS, and Clinical Reports
(diseased products only):
www.allcells.com/info-request
Account Manager support:
www.allcells.com/sales
Technical support:
www.allcells.com/technical-support
Twitter: twitter.com/allcells_bio
Phone: (888) 535-3444, (510) 521-2600
Fax: (510) 521-7600
Mailing address:
AllCells, LLC
1301 Harbor Bay Parkway, Suite 200
Alameda, CA 94502
AllCells Shanghai
Shanghai Ju Ke Science Park
333 Guiping Road
Building 7, 2nd Floor
Xuhui District, 200233
Shanghai, China
Phone: (021)-6485-3421
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
10
General Product Information
statement of ethical standards
We, the staff members of AllCells, LLC, commit ourselves to the following principles, which we recognize are the standards of
ethical behavior and excellence in practice that are fundamental to all activities in biotechnology:
Respect for Human Individuals
We recognize that all humans have inherent dignity and that the welfare, rights, beliefs, perceptions, customs and cultural
heritage of individuals should be taken into account. We treat clients, donors, colleagues, and all others who seek information
about our organization with this respect.
Respect for Animals
Animals have inherent value in their being as well as in their relation to human culture and research. We believe the welfare and
humane treatment of animals should be maintained.
Respect for the Natural Environment
The natural environment is our common heritage and sustains all life. We recognize that the natural environment and the natural
resources of the earth are more than a means to satisfy human ends. The safeguarding of biodiversity, ecosystems,
and the beauty of the natural environment should always be considered for both the present time and in the interest of
future generations.
Respect for the Public Good
We recognize that our individual and group activities occur in the context of social and institutional relationships. The “public
good” is a collective good that should be taken into account along with individual interests. AllCells is committed to ensuring that
this respect is embedded in the way we do business.
Benefit and Harm
We believe human activities have social, economic, and environmental impacts. Human activities should promote good and avoid
harm. At AllCells, LLC, we strive to create a positive impact in these areas and on the scientific research community as a whole.
Justice and Equity
We believe that the benefits and burdens resulting from biotechnological activities should be distributed equitably throughout
society so that no particular group is inequitably advantaged or disadvantaged now or in the future.
Probity
We believe that our activities should be conducted honestly, truthfully, lawfully, impartially, competently, and with considered
regard for transparency of processes.
Accountability
We recognize that individuals and institutions are inherently responsible for their actions and the justification, purpose, and
consequences of any action should be taken into account when determined. We rely on our protocols, policies, and procedures.
The principles noted here provide guidance for the application of ethical behavior in our work as members of the biotechnology
community.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
11
General Product Information
Cell Separation
Purified primary cells are processed by positive or
negative isolation using immunomagnetic
MicroBead labeling systems.
MicroBeads are super paramagnetic particles.
They are conjugated to highly specific antibodies
or proteins. Labeling does not affect viability or
function of the cells. In addition, these particles
have no known effect on structure and do not
interfere with subsequent experiments.
MicroBeads are biodegradable and typically will
detach from the cells within a few days of cell
culture.
Positive Selection: Cells of interest are
magnetically labeled with MicroBeads and
isolated as the magnetically retained cell fraction.
This is the most direct and specific way to isolate
target cells from heterogeneous cell suspensions.
Cell Formats
Primary Cells
AllCells provides a wide range of primary hematopoietic and immunological cells, which are available from
numerous sources, including normal peripheral blood
(NPB), G-CSF mobilized peripheral blood (MPB),
bone marrow (BM), cord blood (CB), and fetal liver
(FL). Primary hematopoietic and immunological cells
have a limited life span in vitro.
Human skin cells, non-human primate, rat, and mouse
primary cells are available as well.
AllCells primary cells are available in fresh, frozen
(cryopreserved) or cell pellet formats.
Please note that the viability of frozen cells after thaw
is normally between the ranges of 80% to 98%.
We guarantee that positively-selected cells have a
purity of >90% (unless otherwise specified).
Negative Selection: Negative or untouched cell
isolation is the depletion of unwanted cells. Nontarget cells are magnetically labeled and
eliminated from the cell mixture by
immunomagnetic separation. The non-magnetic,
untouched cell fraction contains the targeted
cells.
Untouched cells are less likely to be activated by
antibodies. We guarantee that the cell purity is
>85% (unless otherwise specified).
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
12
General Product Information
Lysates
cDNA
AllCells provides fresh primary cells that have been
spun down and lysed in TRIZOL® at 1-5 million
cells/mL or 50-100 million cells/5mL concentration
ranges. The lysed cells are vortexed thoroughly and
kept at -80°C. Lysate products are available by
request only. These special products are meant for
DNA/RNA studies and not meant to be used for
processing after thaw. Please only refer to our
frozen products for these applications.
AllCells cDNA is synthesized from highly purified
RNA, which is derived from high purity
hematopoietic and immunological cells. These cells
are isolated from primary cells using
immunomagnetic MicroBead labeling procedures.
Cell Pellet
Cell Pellets are snap frozen on dry ice. Cell Pellet
products are availably by request only. These
special products are meant for DNA/RNA studies
and not meant to be used for processing after thaw.
Please only refer to our frozen products for these
applications.
Comprehensive RNA
AllCells Comprehensive RNA (CRNA) includes a
combination of Total RNA (>200nt), small RNA and
microRNA (19-200nt) from viable human cells.
These cells are isolated from primary cells using
immunomagnetic MicroBead labeling procedures.
The purity of the cells is analyzed by FACS assay
and is higher than 90%. AllCells uses an optimized
phase extraction method to isolate the freshly
purified cells, which are lysed with TRIZOL®
reagent.
Application
Human primary cell cDNA is developed to meet
researchers’ needs regarding gene expression,
cloning studies, and other experiments in molecular
biology fields.
cDNA Synthesis
The single-strand cDNA is designed for studying
gene expression or cloning genes by PCR
amplification for various types of purified human
cells. RNA is purified from the cells using an
optimized procedure to ensure high quality RNA
that does not contain genomic DNA contamination.
Full-length, high fidelity cDNA is synthesized by a
reverse transcriptase from RNA primed with oligod(T). The original RNA templates are removed from
cDNA using RNase H.
cDNA Purity
AllCells cDNA products are tested using PCR
primers from a list of human housekeeping genes,
such as beta-actin and GAPDH. The PCR product
is examined by the Agilent BioAnalyzer 2100 for
correct product size. In addition, the starting cDNA
material is run through the Agilent Bioanalyzer
2100 for integrity analysis.
RNA Purity
Every qualified lot of AllCells’ CRNA product has
high purity and integrity. Each lot has been validated
by the Agilent 2100 Bioanalyzer. In addition, the
CRNA product is shown to have an intact tRNA, 5s,
5.8s, 18s, and 28s rRNA, and an extremely minimal
presence of genomic DNA. ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
13
Terms & Conditions
Delivery:
General Terms:
1. These outlined conditions regarding sale and delivery from
AllCells, LLC apply to all contracts of supply between AllCells, LLC and our customers.
2. The Customer is deemed to agree to these conditions of sale
and delivery upon acceptance of the goods supplied.
3. Any rights awarded to the Customer arising during the
course of business with AllCells, LLC are not transferable.
4. Any amendment to the conditions of sale and delivery, or any
recognition of the Customer’s conflicting purchase conditions
must be confirmed in writing by AllCells, LLC.
1. All orders are shipped via Federal Express Priority
Overnight delivery unless otherwise specified.
2. Estimated delivery times can be specified. However,
estimated delivery times are not legally binding.
3. Same day delivery is available in the San Francisco Bay
Area with prior arrangement with AllCells, LLC.
4. All deliveries take place at the Customer’s risk. The
transfer of risk occurs upon the handing over of the goods
to the carrier or shipper. The course of delivery method is
at the discretion of AllCells, LLC with exclusion of any and
all liability.
Prices:
Payment:
1. Payment of goods is to be made within 30 days of delivery
1. AllCells, LLC prices are subject to change without prior noand remittance of the bill.
tice. Please call, fax, or email to confirm current pricing with
2.
Invoices not paid within the designated terms are subject
AllCells, LLC.
to a 10% late fee of the initial balance and a 1.5% per
2. All shipping and handling charges will be reflected on the
month (18% annum) interest fee.
final invoice. Shipping and handling charges will vary accord3.
Failure of payment within 120 days may result in legal and
ing to the weight of the package and the shipping options.
collection proceedings upon the expiration of the payment
Please contact AllCells, LLC directly for current shipping and
period without further warning.
handling prices.
4.
AllCells, LLC accepts only US currency in the form of
3. Prices quoted by AllCells, LLC are net prices. Any sales tax,
company checks, cashier checks, money orders, and VISA,
use or excise tax, custom duties or other fees imposed upon
American Express, MasterCard payments. All funds must
a transaction by any governmental authority shall be paid by
be withdrawable from a US bank account; foreign bank
the customer in addition to the price quoted and invoiced.
accounts and foreign currency will not be accepted as
appropriate payment. Please make checks payable to
Orders, Cancellation, and Rescheduling:
AllCells, LLC.
5.
All international orders must be prepaid in advance with
1. Once you have placed your order, AllCells will recruit and
either a bank deposit or credit card before shipment.
schedule donors for tissue collection according to your
6.
If AllCells, LLC payment conditions are not complied with,
requirements.
or if justifiable concerns exist in regard to the Customer’s
2. Fresh product orders which are cancelled or rescheduled
ability or willingness to pay, AllCells, LLC is entitled to
within the Lead Time Period required for each collection will
deem any contract with the Customer null and void and
incur a 10% Cancellation/Rescheduling Fee according to
demand the return of the delivered goods in the condition
the Table below. For example, Lead Time Period for Bone
in which they were shipped. The cost of this return of
Marrow collection is 2 weeks, therefore if order is cancelled
delivery is the responsibility of the Customer. In addition,
or rescheduled within 2 weeks from the scheduled collecall outstanding claims the Customer may have with
tion time, customer will be charged a 10% Cancellation/
AllCells, LLC will become due.
Rescheduling Fee.
7.
Justifiable concerns referred to above include, but are
3. Orders may not be cancelled within 24 hours of the schednot restricted to the following: initiation of insolvency or
uled collection time.
bankruptcy proceedings, deterioration of financial circumProduct Format* Product Type
Lead Time Period
stances, and default of payment for any other reason.
Fresh
Frozen
ALLCELLS
Mobilized Leuko Pak 4 weeks
Leuko Pak
2 weeks
Bone Marrow
2 weeks
Whole Blood
1 week
All Products
1-2 business days
Toll Free Phone: 1-888-535-3444
*Lead times apply to healthy donor products only.
Diseased tissue and cells are limited by stock
availability. Lead times may vary due to the nature
of the disease and sensitivity of patients.
www.allcells.com
For In Vitro Research Use Only
14
Terms & Conditions
Use of Goods:
1. All products received from AllCells, LLC are intended
exclusively for in vitro use only. AllCells, LLC products
are not intended for use in human beings, animals, or for
any private use. Please note that any use of AllCells, LLC
products for any unintended purposes (including use
for therapeutic or diagnostic purposes) will have legal
ramifications.
2. AllCells, LLC does not accept any liability for the
improper use of our products. We deliver our products
only to research and teaching institutions, commercial
enterprises, and public researchers. Any signs of improper
use of AllCells, LLC products by a customer will result
in refusal of delivery and termination of any contract
associated with that customer.
3. AllCells, LLC opposes any research in violation of any
ethical, legal, or governmental standards within the United
States and the import countries of our customers. We do
not accept any liability for such research or use by our
customers.
Liability:
1. AllCells, LLC represents and warrants that it has (1)
obtained ownership rights with respect to products and
that such products were provided to AllCells, LLC with
every donor’s informed consent and in compliance with all
applicable laws and regulations, (2) obtained all necessary
and appropriate releases from the donor wherein
there are no restrictions on use of products that would
prohibit customers from using them in any commercial
pharmaceutical research conducted in vitro, (3) complied
with all applicable United States and foreign statutes and
their respective rules and regulations in connection with
handling, export, import, and use of the products.
2. AllCells, LLC represents and warrants that it has no
obligation to any third party that would interfere with
AllCells, LLC providing products to customers. In addition,
any products that AllCells, LLC provides to customers are
not the proprietary property of any third party.
3. In no event will AllCells, LLC be liable to a customer or
anyone else for any damages or injury resulting from the
use or misuse of our products, nor for any action taken by
a customer relying on information, either verbal or written,
from any source.
4. The Customer agrees that AllCells, LLC’s liability, if any,
for damages (including liability, arising from physical or
mental harm, caused by any product liability, arising out of
contract, negligence, strict liability, or tort) shall not exceed
the cost of the product, which the Customer purchased
from AllCells, LLC.
5. The Customer agrees to indemnify and to hold harmless,
ALLCELLS
Toll Free Phone: 1-888-535-3444
AllCells, LLC and all other parties involved, including their
owners, officers, affiliates, associates, and employees
for any and all damages incurred as a result of the
products used. These parties shall not be liable under any
circumstances.
6. By ordering any products from AllCells, LLC, the Customer
acknowledges the above terms, conditions, and disclaimers,
included but not limited to the above disclaimers and any
additional warnings and cautions. The Customer agrees to
carefully abide by these terms, conditions, and disclaimers.
Warranty:
1. AllCells, LLC warrants that our products will meet the
claimed product specifications, such as viability, number of
viable cells, and purity. AllCells, LLC does not warrant any
biological properties associated with our products.
2. AllCells, LLC will replace any products that do not meet the
specifications claimed on our certificate of analysis free of
charge. In no event shall AllCells, LLC’s liability exceed the
purchase price paid by the Customer.
3. AllCells, LLC shall not be liable for any damages or injuries
to persons or property arising from the use of its products. AllCells, LLC is not liable for a product that has been
misused or has been rendered unusable due to improper
storage or handling.
4. AllCells, LLC products are “For In Vitro Research Use Only”
and are approved neither for human nor veterinary use in
vivo, nor for any diagnostic or clinical applications.
5. This limited warranty has a one-year period starting from
the time of shipment.
6. AllCells, LLC’s products are not permitted for re-sale
without written authorization from AllCells, LLC.
Return Policy:
Catalog products may not be returned for credit without
the express prior written authorization of AllCells, LLC.
A Restocking Fee will be applied to returns in which the
purchaser is at fault. A Restocking Fee of $50 or 15% of
the purchase price will be applied, whichever is greater.
Returns involving fresh cell products must be requested
within 24 hours of product delivery. Returns involving
frozen cell products must be requested within 6 months of
product delivery. Products must be returned in their original
containers with original AllCells label affixed and unaltered.
For product returns to be considered, customer must certify
that product was maintained under recommended storage
conditions while in its possession, and will be packaged and
delivered back to AllCells according to the same conditions
as the original shipment. Final acceptance of returned
product is at the sole discretion of AllCells, LLC. No returns
will be accepted for custom product orders.
www.allcells.com
For In Vitro Research Use Only
15
TISSUE Collection facility
Product Description
Photo credit: Miltenyi Biotec CliniMACS®
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
16
TISSUE COLLECTION FACILITY
Background
AllCells, LLC has been operating its own private tissue collection facility under IRB-approved protocols since 2007,
following a 10-year partnership with a local community hospital. Our facility employs a staff of registered nurses each
with over 15 years of blood collection experience from healthy donors. A licensed physician, board certified in the
specialties of hematology and oncology, is the medical director and principal investigator for all our healthy donor related
cell collection studies.
We are committed to making the safety and well-being of our donors our first priority. Participating donors receive safe
and attentive care guided by community standards, the FDA, and the California State Department of Health regulations
for blood product donation.
We are dedicated to providing donors with a confidential experience. Donor identity is rigorously protected observing
federally-recognized standards. All staff members are experienced and compliant regarding the protection of human
research subject health information and privacy issues per the Health Insurance Portability and Accountability Act of
1996 (HIPAA) Privacy and Security Rules.
Equipment
A complete blood count (CBC) blood sample is collected from all donors prior to blood and/or bone marrow donation to
verify donor eligibility. The samples are analyzed on a HoribaABX Micros 60 blood analyzer with results available in
about one minute.
All apheresis cell separation procedures are completed by specially trained registered nurses using the COBE® Spectra
Apheresis System (hardware, software, and disposable kits) by Terumo BCT, Lakewood, CO.
Whole blood collections are completed using a 17-gauge arterio-venous fistula needle. A local anesthetic is applied to
the IV site first for donor comfort. Samples are collected into receptacles of the customer’s choice, such as 600 ml blood
transfer packs, 10 mL EDTA tubes, or other types of vacutainer tubes, 60 mL heparinized syringes, etc. Customers may
request specific collection receptacles, or provide specific collection receptacles directly to AllCells, LLC if they prefer.
Bone marrow aspirations are performed by physicians only and are collected with a 15-gauge or 16-gauge beveled bone
marrow aspiration needle (Ranfac or equivalent). A local anesthetic is applied to the donor’s skin and aspiration site first
for donor comfort.
IRB
All cell collection protocols are overseen and approved by an independent Institutional Review Board (IRB) on an annual
basis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
17
TISSUE COLLECTION FACILITY
Mobilization
AllCells has been a pioneer in the use of G-CSF (Neupogen®) stimulation in normal healthy donors since 1998. The
protocols in use for our Mobilized Peripheral Blood (MPB – pg. 61) products have been approved by an IRB and are
overseen by clinical physicians. In addition all donors are monitored during the injection, collection procedure and
post-collection to ensure the health of our donors is a priority. AllCells is one of only a few providers of Mobilized
Peripheral Blood in the world.
In addition AllCells now offers Plerixafor (Mozobil®) mobilization of stem cells from healthy donors. Mozobil, a selective
CXCR4 antagonist, has been particularly beneficial as an adjuvant stem cell mobilization agent on top of the G-CSF
regimen. The Mozobil regimen requires only 1 injection as opposed to the G-CSF 3-5 day protocol. AllCells is currently
the only source of Plerixafor-mobilized cells in the world. For more information on this exciting new mobilization agent
visit our Mobilized Peripheral Blood section on page 61.
Donor Criteria
Non-mobilized Products•
Age 18-55 years old
•
Donors cannot be pregnant
•
Weight > 110 lbs for whole blood >140 lbs for Leuko Pak
•
Donors must be in good health
•
Complete Blood Count lab test must meet protocol specs
•
Donors are required to sign consent form prior to collection
•
Donors are screened for HIV (HIV 1 & 2 Ab),
•
All consent and protocols approved by an IRB
Hepatitis B (Surface Antigen HbsAg) & HCV (HCVAb)
Mobilized Products•
All Non-Mobilized criteria
•
Minimum 12 months between donations
•
Age 18-40 years old
•
Twice-in-a-lifetime maximum donation
Capabilities
Having our own tissue collection facility allows us to customize a variety of project variables including protocols, techniques, equipment, donor characteristics, and delivery.
Customization
Bone Marrow
Apheresis
Custom
Collection
Protocols
•
•
•
•
•
Donor
Customization
•
•
•
Other
Customization
ALLCELLS
Anticoagulant type ( i.e.
Heparin, ACDA)
Syringe size
Demographics (i.e. age,
ethnicity, gender, etc.)
Past medical history (i.e. health
habits, allergies, medications,
smokers vs. non-smokers, etc.)
HLA typing
ABO typing
Same day delivery available for local
customers
•
•
•
•
•
Whole Blood
Mobilized vs. Non-mobilized
Product volumes (i.e., cells
vs.autologous plasma)
Product Hematocrit
Demographics (i.e. age,
ethnicity, gender, etc.)
Past medical history (i.e. health
habits, allergies, medications,
smokers vs. non-smokers, etc.)
HLA typing
ABO typing
Same day delivery available for local
customers
Toll Free Phone: 1-888-535-3444
www.allcells.com
•
•
•
•
•
•
Anticoagulant type (i.e. Heparin,
EDTA, ACDA)
Collection receptacles (i.e,
heparinized syringes, vacutainer
tubes, transfer packs, etc.)
Demographics (i.e. age, ethnicity,
gender, etc.)
Past medical history (i.e. health
habits, allergies, medications,
smokers vs. non-smokers, etc.)
HLA typing
ABO typing
Same day delivery available for local
customers
For In Vitro Research Use Only
18
TISSUE COLLECTION FACILITY
Standard Collection — Lead Times
When placing your order with our team please keep the following lead times in mind for our standard collection
products:
Collection Type
Lead Time
Mobilized Peripheral Blood (Leuko Pak)
4 weeks
Normal Peripheral Blood (Leuko Pak)
2 weeks
Bone Marrow Aspiration
2 weeks
Whole Blood
1 week
Matched Sample Collection
In addition to the capabilities illustrated here, AllCells has the ability to collect multiple types of tissues from the same
donor on the same day. Various combinations of product types can be mixed and matched including Bone Marrow, Whole
Blood, and Apheresis. Cetain restrictions apply and 3-4 weeks advance notice is required. Please see our ‘Combo Pak’
product information on page 36 for more information or contact us directly.
Inquire with us for your specific research needs. You may send us an email at [email protected] or fill out our
‘Project Request Form’ online at: www.allcells.com/technical-support/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
19
bioservices
Photo credit: Miltenyi Biotec autoMACS Pro®
Photo credit: BD FACSCanto II®
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
20
BIOSERVICES
Bioservices Table of Contents
Immunoassays21
Hematopoietic Assays22
Flow Cytometry Services
23
Tissue Culture Services
24
Protein Analysis25
Genotyping 26
Custom HLA-Typing27
Your Trusted Partner in Hematology Based Research & Development
Since 1998, AllCells has been the industry leader in providing high quality hematopoietic and immunological cell products to leading
researchers. Our scientists have over 50 years of combined experience developing a wide range of hematopoietic assays. We are
proud to offer the following services as part of our Bioservices division:
Immunoassays
Protein Analysis Services
•
ELISA (single, multiplex)
•
Protein Electrophoresis
•
ELISpot
•
Western Blot
•
Luminex® (single, multiplex)
•
Immunoprecipitation
•
Human Donor HLA Typing
Genotyping
Hematopoietic Assays
•
gDNA Extraction
•
Hematopoietic Stem Cell Based Assays (Colony Formation)
•
Mutation Analysis
•
CD16 Genotyping
—CFU-G, CFU-M, CFU-GM, BFU-E
—CFU-Mk
Custom HLA-Typing
—LTC-IC
•
Mesenchymal Stem Cells/Multipotent Stromal Cells (MSC)
•
HSC/Progenitor differentiation
—Megakaryocytes, Dendritic cells
Flow Cytometry Services
•
Multivariable Flow Cytometric Analysis, up to 11 colors
•
Immunophenotyping (cell surface and intracellular)
•
DNA Analysis
•
ISHAGE
—CD34 enumeration
—Leukocyte characterization
Tissue Culture Services
•
Luminex® Multianalyte Assays
•
In vitro Proliferation/Inhibition Assays (EC50)
•
CFSE
•
Cell Proliferation Assays (viability, cell counting)
•
MTS/MTT/CTG
•
Toxicology Studies
•
Diseased Cells Analysis
For more information visit: www.allcells.com/bioservices/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
21
BIOSERVICES
Immunoassays
As part of our Bioservices offering, AllCells specializes in Immunoassays. Immunoassays are a robust method of detecting cellular
response and assessing drug efficacy through protein expression/secretion. These assays have industry applications in autoimmunity, transplantation, vaccine development, therapeutic development, and many others. AllCells provides immunoassay services
including ELISA, ELISpot, Luminex, and Human Donor HLA Typing amongst others.
Enzyme-linked immunosorbent assay, or ELISA, is a quantitative assay that can be used to characterize expression levels of
soluble proteins. This assay features a high throughput, scalable, easily-read platform. Due to its flexibility, ELISA can be applied to
numerous targets depending on your specific requirements.
The following is representative of a typical standard curve output from our ELISA assay, illustrating good upper and lower limits of
detection: (Data Set 1, Data Set 2, Data Set 3)
ELISpot
Enzyme-linked immunospot assay, or ELISpot, is a colorimetric assay which enables quantitative, single cell analysis of protein
expression levels. Due to its high sensitivity and reproducibility, the ELISpot is an industry standard in antigenic response in the form
of cytokine secretion.
Luminex (single, multiplex)
The Luminex Bead Array System allows for a high throughput, multi-target analysis of cytokines, proteins, and nucleic acids for
researchers. The system is important for many clinical and research applications. Our Bioservices team can provide customized
applications of the system to accelerate your research. Inquire with us today by filling out our Technical Support form at www.allcells.
com/technical-support/.
Human Donor HLA Typing
HLA (Human Leukocyte Antigen) Typing of donors is important to reduce variability in research studies conducted within the field of
immunology, including inflammation, immune response and antigen presentation. AllCells Bioservices provides HLA Typing of our
donors for specific A2, DRB4 loci. We offer this service based on the specific research criteria you may have. Please inquire with us
today about our HLA Typing capabilities and how we may assist with your project needs.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
22
BIOSERVICES
Hematopoietic Assays
As a leader in the provision of hematological samples to the research community, AllCells has developed over 20 years of experience in performing hematopoietic stem cell assays. AllCells offers services in colony formation assays and the expansion and
differentiation of stem and progenitor cells, including hematopoietic and mesenchymal stem cells.
Hematopoietic Stem Cell (HSC) potential is routinely measured through myeloid colony forming units (CFU) in a semi-solid
culture environment. In the presence of cytokines, unipotent progenitor cells are capable of giving rise to colonies with granulocytes
(CFU-G), macrophages (CFU-M), erythrocytes (BFU-E) and megakaryocytes (CFU-Mk) while stem/progenitor cells with multipotency give rise to colonies with multiple lineages (CFU-GM and CFU-GEMM). Long term culture-initiating cell (LTC-IC) assays are
used to identify the most primitive HSC which is capable of maintaining its multipotency after long term culture.
Mesenchymal Stem Cells/Multipotent Stromal Cells (MSC) are isolated by AllCells from multiple sources, including bone
marrow, cord blood, adipose tissue and dental pulp from deciduous teeth. MSC have been shown to possess multilineage differentiation potential, including but not limited to adipocytes, chondrocytes, osteoblasts, neurons and myocytes. AllCells offers custom
services for the quantification of MSC using colony forming fibroblast (CFU-F) assays and characterization of MSC derived
adipocytes, chondrocytes and osteoblasts.
In addition to characterization of HSC and MSC potential, AllCells offers services in custom differentiation of our stem and
progenitor cells. AllCells can provide our customers with populations of difficult to isolate cells which have been expanded and
differentiated in culture, including megakaryocytes, dendritic cells, mast cells, and early erythroid cells.
Case study: Samples were treated with different compounds, cultured, and colonies were manually counted. Graph below displays
various colony types after compound treatment.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
23
BIOSERVICES
Flow Cytometry Services
AllCells utilizes our 20+ years of Flow Cytometry experience and state-of-the-art equipment to provide a customized research
solution. We offer Multivariable Flow Cytometric Analysis with up to 7 colors simultaneously using our FACSCalibur®, FACSCanto II® machines. We also offer custom Multivariable Cell Sorting using our FACS Aria II®. Our services include Immunophenotyping of intracellular and extracellular molecules, DNA Analysis, and CD34 Enumeration and Leukocyte Characterization using
ISHAGE guidelines.
Our Immunophenotyping services can be applied to cellular characterization of both intracellular and extracellular molecules using
fluorescently-labeled antibodies. These include but are not limited to MHC/HLA Typing, Cancer Cell Analysis (focusing on Hematological Malignancies), and other custom analyses.
Our DNA Analysis services can provide information on the percentage of cells in each phase of the cell cycle. This output can be
used in drug screening applications, inhibition, proliferation, senescence, etc.
Flow-cytometric enumeration of CD34+ hematopoietic stem and progenitor cells (HSCs) is widely used for evaluation of graft
adequacy of peripheral blood and bone marrow stem cell grafts. The International Society of Hematotherapy and Graft Engineering
(ISHAGE) has created guidelines for a gating strategy that results in replicable standards for stem and progenitor cell enumeration.
AllCells Bioservices adheres to ISHAGE guidelines for characterization of leukocytes and quantification of CD34+ cells.
Fluorescence-activated Cell Sorting (FACS) utilizes the principles of flow cytometry to identify populations of cells of interest.
Cells can be electrically charged and sorted into highly purified populations. At AllCells, we are capable of identification and
sorting of cell populations using up to 11 colors.
Our Bioservices team can provide customized applications of the system to accelerate your research. Inquire with us today by filling
out our Technical Support form at www.allcells.com/technical-support/.
Case Study: Extracellular LSK markers were placed on mouse bone marrow cells. LSK markers are an indication of mouse
hematopoietic cells (HSCs) in the mouse bone marrow.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
24
BIOSERVICES
Tissue Culture Services
AllCells Bioservices includes Tissue Culture Services to help study kinetics of cellular response to external stimuli. We offer a wide
range of platforms for analysis of cell proliferation and viability. This can include MTT, MTS, CTG, and Annexin V assays.
Tissue Culture Services include:
•
Luminex® (single, multiplex)
•
In vitro proliferation/inhibition assays (EC50)
•
CFSE (refer to Flow Cytometry Services pg. 23)
•
Cell proliferation assays (viability, cell counting)
MTS/MTT/CTG – To study the kinetics of cell viability in drug compound screening, we are able to implement specific assays.
Alternatively, these assays may be used for inhibition studies, illustrating the kinetics of your cells: EC50/IC50 analysis
Toxicology Studies – Our bioservices can help with QC validation of toxic effects on cells by certain compounds.
Diseased Cells – AllCells serves as a reliable source for a variety of hematological, oncologic, and autoimmune diseased cells that
can be used with multiple bioservices including compound screening, biomarker discovery, and characterization of specific
diseased cells. We source our own cells, have control over our supply, and can customize based on research demands.
Compound treatment is one example of our Tissue Culture Services:
Proliferation - grow cells, add reagent,
incubate, measure live cells labeled
Apoptosis (Annexin V/PI)
Compound Treatment - aliquot
cells, treat with drug compounds
Pathway Activation
CFU-C Assay
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
25
BIOSERVICES
Protein Analysis
AllCells is currently offering custom services in Protein Analysis, to compliment your research. AllCells has a protein analysis
platform that includes services in Protein Electrophoresis, Western Blot and Immunoprecipitation. Our Bioservices team features
over 15 years of combined Protein Analysis experience.
Protein Electrophoresis
Protein Electrophoresis utilizes the differences in size and charge of proteins for separation and detection in a polyacrylamide gel.
Protein electrophoresis is a highly reliable method for determining the composition and relative quantities of proteins in cell samples.
Western Blot
For characterization of specific protein expression levels, further Western Blot analysis can be performed by transferring electrophoresis separated proteins from the polyacrylamide gel onto a membrane. Semi-quantitative analysis can be performed on protein
expression levels in samples.
Immunoprecipitation
To determine which proteins are interacting in your sample, we offer immunoprecipitation services for isolation of proteins and their
binding partners. In addition our Bioservices team can optimize your immunoprecipitation process.
Our Bioservices team can provide customized applications of our system to accelerate your research. Inquire with us today by filling
out our Technical Support form at www.allcells.com/technical-support/.
Case Study: Intestinal primary myofibroblasts of mice were isolated. The cells were then analyzed using Western Blot to determine
if the cells expressed NPR-C. Figure below is the output illustrating the expression of NPR-C with Actin control:
NPR-C:
Actin:
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
26
BIOSERVICES
Genotyping
As part of AllCells Bioservices we now offer genotyping services. Our genotyping services focus on genomic DNA extraction for
mutation analysis, HLA genotyping, and Fc-gamma Receptor IIIa (CD16a typing). Genotyping services are useful for minimizing
variation in your sample population to increase reproducibility in downstream applications.
gDNA Isolation
Our Bioservices can package genomic DNA extraction with mutational analysis, genomic profiling, and other sequence-based
analyses.
The advancement in genetic engineering has transcended genomic DNA from the basic code of life to the pivotal tool for
next generation medicine. From gene therapy to cellular reprogramming, the DNA molecule continues to manifest its dynamic
potentials.
Because the research needs for genomic DNA may or may not be cell-type dependent, AllCells offers a customized service to extract
and purify genomic DNA from any of our available primary cell products, human as well as other available mammalians, and from any
other mammalian tissues such as human tumors provided by the research client.
AllCells also features cell-type and donor matched extractions of both genomic DNA and comprehensive RNA. Pricing may vary.
Please contact us today by filling out our Technical Support form at www.allcells.com/technical-support/for related inquiries and
requests.
Mutation Analysis
Can be used to detect abnormalities in cell populations. Examples of our mutation analysis are CD16-typing and HLA-typing. We
implement PCR-based assays, PCR genotyping with sequencing where needed. We source our own cells, have control over our supply, and can customize based on research demands. We can also provide KIR-Typing for NK cells.
HLA Genotyping – For more information please refer to our Custom HLA Typing section on the following page.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
27
BIOSERVICES
Custom HLA-Typing
Human Leukocyte Antigen, or HLA, plays a strategic role in the recognition and defense mechanisms of the human body.
The segregation and pooling of the human genome have created a wide spectrum of diverse HLA. Many research efforts are
underway to employ different HLA haplotypes for aid in identifying treatment for infectious diseases, autoimmune diseases
and cancer.
In a more direct area of medicine, recombinant and chimeric monoclonal antibodies (MAb) technologies have become the latest super weapon against cancer, autoimmune and infectious disorders, and even graft rejection, through enhanced pathways
of ADCC (antibody dependent cell-mediated cytotoxicity) and CDC (complement dependent cytotoxicity). One of the critical
factors for mAB efficacy is its Fc portion's affinity to the effector cell's Fc receptor. One such receptor, the FcG RIIIA(CD16),
is subjected to a single nucleotide polymorphism which results in formations of F (Phenylalanine) or V (Valine) at amino acid
position 158, and causes different levels of affinity to the Fc portion of IgG. Recent breakthroughs in antibody engineering
saw the ability to possibly overcome such affinity issues. However, the quest for the perfect monoclonal antibody continues,
and AllCells welcomes the opportunity to work with all of the antibody experts in anyway we can, from cell characterization to
custom assays.
Product/Service Descriptions
HLA-A2 and HLA-B27 expressions are tested by leukocyte surface immunophenotyping. For higher resolution haplotypes,
HLA-A0201 and HLA-DRB1*0401 are analyzed by sequence specific priming PCR.
FcG-RIIIA-158 allelic expression is characterized by detection of CAPS, or Cleaved Amplifed Polymorphic Sequence. We routinely test
our human donor samples for these markers, and can provide testing services for new donors upon request.
Product/Service Type Immunological
Method
Catalog #
HLA-A2+
Immunophenotyping
C-HLA-A2
HLA-B27+
Immunophenotyping
C-HLA-B27
HLA-A*0201+
SSP Genotyping
C-HLA-A0201
HLA-DRB1*0401+
SSP Genotyping
C-HLA-DRB10401
Fc Gamma Receptor
IIIA-158-FF
Fc Gamma Receptor
IIIA-158-VF
Fc Gamma Receptor
IIIA-158-VV
CAPS
C-FCG-R3A158-FF
Price
Target
Immunologically
Characterized
Stock Products
ALLCELLS
Toll Free Phone: 1-888-535-3444
Please contact us for
pricing
C-FCG-R3A158-VF
C-FCG-R3A158-VV
www.allcells.com
For In Vitro Research Use Only
28
Human Primary Cells
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
29
HUMAN PRIMARY CELLS
Human Primary Cells & Tissue Table of Contents
Custom Products30-32
New Products33
Normal Peripheral Blood
35-60
Mobilized Peripheral Blood
61-68
Bone Marrow69-78
Cord Blood
79-95
Fetal Liver96-102
Non-Hematopoietic Stem Cells
103-106
Diseased Cells107-154
Human Primary Cells & Tissue
Human cells & tissue are the most clinically-relevant model for research. AllCells provides a wide range of primary human
cells. These human primary cells include hematopoietic and immunological cells, mesenchymal cells, epidermal cells, amniotic
and placental cells, and dental pulp cells.
These human cells are made available from numerous sources. Our normal/healthy human cells are sourced from healthy
peripheral blood, G-CSF mobilized peripheral blood, bone marrow, cord blood, fetal liver, amniotic & placental fluid, dental pulp,
and skin. Our diseased human cells are sourced from either peripheral blood or bone marrow.
AllCells human cells product line is available in fresh or frozen format. Please contact us for any special requests or custom
isolations at www.allcells.com/technical-support/ or [email protected].
For more information visit: www.allcells.com/human/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
30
Custom products
AllCells is able to offer a variety of customized projects and services. Our Custom Product/Project Request Form appears on the following pages. For more information see our Tissue Collection
Facility Capabilities on page 17.
Examples of custom primary cell isolations include:
•
Custom cell numbers supplied per unit
•
Custom processing/packaging:
-Frozen or snap frozen for RNA or cDNA
-Lysis with custom buffer/reagents
-Transfer into tubes/bags
-PAXgene/Cyto Chex tubes
-Transfer bags
-Custom handling
•
Multiple cell types from a single donor
•
Donor-matched cells
-Matching plasma from the same donor
-Blood typed donors
•
Hematopoietic stem cells from mobilized peripheral blood
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
31
CUSTOM PRODUCTS
Custom Product/Project Request Form
Our team can provide custom research solutions depending on your project requirements. The following form will be used by
our team to help determine how best we can serve you and accelerate your research. Please fill out the form online and a
Technical Support Representative will respond usually within 24 hours. The form can be found under the Custom Product/
Project category at www.allcells.com/technical-support/.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
32
CUSTOM PRODUCTS
Custom Product/Project Request Form (cont'd)
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
33
new products
Dual Half Pak & Combo Pak
Due to the increased popularity of the Leuko Pak product line, AllCells has launched our latest offerings, the Dual Half
Pak & the Combo Pak. For the Dual Half Pak product, a healthy donor undergoes an apheresis procedure twice within
a short 6-12 week interval. Each time half of a Leuko Pak is processed. For the Combo Pak AllCells offers the option to
obtain Bone Marrow, Whole Blood, and a Full Leuko Pak from the same donor at the same time. Any and all combinations of these products may be available. Inquire with us today to learn more: [email protected].
See page 36 for more information.
Adipose-Derived Stem Cells
AllCells’ adipose-derived stem cells (ASC) are isolated from the stromal vascular fraction of lipoaspirates (obtained
by conventional vacuum-assisted or ultrasound –assisted liposuction methods) through culturing on plastic. ASCs are
cultured up to third passage, harvested, and characterized. They can be identified by means of fluorescence-activated
cell sorting, as they have several specific surface markers: CD73+, CD90+, CD105+, CD45–, CD34–, CD14 or CD11b,
CD79– or CD19–, and HLA-DR6. Fresh cells, stromal vascular fraction, and lipoaspirates are available upon request.
For more information see page 103 within our Non-Hematopoietic Stem Cells & Tissue section.
AllCells Cryopreservation Media
AllCells has begun manufacturing our own cryopreservation media,
specially formulated to work best with our primary cell products. This
media contains DMSO and proprietary reagents that increase the
viability of cryopreserved cells. For more information see page 173.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
34
HEALTHY Primary Cells & TISSUE
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
35
Normal Peripheral Blood
Normal Peripheral Blood Table of Contents
Apheresis Collection (Leuko Pak)
36
Whole Blood37
Plasma38
Mononuclear Cells (MNC)39
Stem/Progenitor Cells (CD34+)40
Lymphoid Cells
B Cells41-43
(CD19+, CD19+/IgD+, Memory)
T Cells44-50
(CD3+ Pan, CD4+ Helper, CD4+/CD25+,
CD4+/CD45RA+/CD25- Naive Helper,
CD4+/CD45RO+ Memory, CD8+ Cytotoxic,
CD8+/CD45RA+ Naive Cytotoxic)
Dendritic Cells (BDCA4+ Lymphoid/Plasmacytoid)
51
Natural Killer Cells (CD56+)52
Myeloid Cells
Basophils53
Eosinophils54
Neutrophils55
Red Blood Cells/Mature Erythrocytes 56
Platelets 57
Monocytes (CD14+)58
Dendritic Cells (Monocyte-Derived)
59
Macrophages60
Donor Criteria
•
Age: 18-55 years old
-Blood and bleeding disorders, including sickle cell disease
•
Weight: >140 lbs for leuko pak
-Neurologic disorders
•
Weight: > 110 for whole blood
-Autoimmune disorders
•
CBC lab tests must meet protocol specifications.
-Cancer
•
Donors cannot be pregnant.
-Diabetes
•
Donors are screened for HIV, Hepatitis B and Hepatitis C
-Positive: HIV, HbsAg, HCAb
•
before donation.
•
Donors must have adequate peripheral veins for apheresis.
Donors must be in general good health. Donors with any of the
•
Donors are required to sign a procedure-specific consent form
following will be excluded from donating:
prior to the collection procedure.
-History of heart, lung, liver, or kidney disease
-History of asthma
Lysate and Cell Pellet products are available upon request.
For more information visit: www.allcells.com/normal-peripheral-blood/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
36
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB007-2
1/4 Bag
$1566
PB007-1
1/2 Bag
$1874
PB007
1 Bag
$2801
PB007-3A
1st 1/2 Bag
$2858
PB007-3B
2nd 1/2 Bag
$2350
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Leuko Pak
Product Description
Application
AllCells Leuko Paks are highly-enriched leukapheresis-derived
products obtained from our own IRB-approved collection facility. These products contain higher concentrations of mononuclear cells, B cells, T cells, stem/progenitor cells, dendritic
cells, and other cell types as compared to standard venipuncture collection methods or buffy coat products.
Full/Half/Quarter Pak - Normal peripheral blood mononuclear
cells are collected from healthy donors in various collection
sizes. For the Full Leuko Pak, approximately 3 blood volumes
are processed using the COBE SpectraTM apheresis machine
and between 50 and 150 mL of autologous plasma is collected at the same time as the mononuclear cells.
Dual Half Pak - PB007-3A, -3B - A healthy donor undergoes
an apheresis procedure using the COBE SpectraTM machine
on two separate occasions. The same donor is reserved for a
second apheresis procedure 6-12 weeks after the first. Each
time, 1.5 blood volumes are processed and approximately 100
mL of normal peripheral blood mononuclear cells and 50 mL of
autologous plasma are collected. Second Half Pak from same
donor is optional.
Combo Pak - AllCells also provides an option to obtain Bone
Marrow (50 or 100 mL), Whole Blood (up to 120 mL), and a
Full Leuko Pak from the same donor at the same time. Any and
all combinations of these products are available. Inquire for
more information.
Leuko Pak
¼ Bag: ½ Bag: 1 Bag: Cell Number Ranges
Average: 0.37x1010
Average: 0.75x10 10
Average: 1.5x10 10
NPB Leuko Paks can be used to:
• Isolate large-scale dendritic cells.
• Profile and screen normal peripheral blood monocytes
and lymphocytes.
• Study intercellular interactions by using the same-donor
leukocytes directly or in culture.
Lead Time
A minimum of two weeks is needed.
Related Products
G-CSF Mobilized Peripheral Blood Leuko Pak
Published Papers
Vose, Sara C., et al. (2007) Lysophosphatidylcholine Hydrolases of
Human Erythrocytes, Lymphocytes and Brain: Sensitive Targets of
Conserved Specificity for Organophosphorus Delayed Neurotoxicants.
Toxicol Appl Pharmacol., 224(1), 98–104.
http://www.allcells.com/publications/0089allcells.pdf
Lu, Xiaobin Lu, et al. (2004) Antisense-Mediated Inhibition of Human
Immunodeficiency Virus (HIV) Replication by Use of an HIV Type
1-Based Vector Results in Severely Attenuated Mutants Incapable of
Developing Resistance. Journal of Virology, 78(13), 7079–7088.
http://www.allcells.com/publications/0043allcells.pdf
Range: 0.1 to 0.7x1010
Range: 0.6 to 1x1010
Range: 1 to 3.5x1010
For more information visit: www.allcells.com/leuko-pak/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Leuko Pak (Apheresis Collection)
37
Normal Peripheral Blood
Normal Peripheral Blood—Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
WB001
60 ml
$328
WB002
120 ml
$585
WB003
180 ml
$818
WB004
240 ml
$1055
WB005
300 ml
$1289
Comprehensive RNA
RNA-WB001C
15 µg
$685
cDNA
cDNA-WB001
10 PCR rxns
$314
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Whole Blood
Lead Time
Product Description
Whole blood (WB) is anti-coagulated using heparin, ACD:A or
EDTA per customer specification. Our standard anticoagulant
is heparin (5 U/ml of WB). The volume of whole blood removed
per donation may vary, but will not exceed 300 mL at any
one time. Whole blood collection quantities are in volume
increments of 10 mL up to a maximum of 300 mL Please
contact us if smaller volumes are needed. WB can be collected
in vacutainer tubes, syringes, or transfer packs.
Application
Whole Blood can be used to study:
• Diagnostic or early detection tools for different diseases,
blood-related or unrelated.
• Serum and plasma proteins and their pathway functions,
such as metabolism and immunity.
• Therapeutic antibodies or other drug screenings.
A minimum of one week is needed.
Published Papers
Noto, Paul B., et al. (2012) Regulation of Sphingomyelin Phosphodiesterase, acid-like 3A gene (SMPDL3A) by Liver X Receptors.
Molecular Pharmacology, 112.078865.
http://www.allcells.com/publications/0162allcells.pdf
Park, Eun-Jin, et al. (2008) Improved Bone Healing by Angiogenic
Factor-Enriched Platelet- Rich Plasma and Its Synergistic Enhancement by Bone Morphogenetic Protein-2. Intl J Oral Maxillofac
Implants, 23 (5), 818-826.
http://www.allcells.com/publications/0081allcells.pdf
Zhao, Zhiwei, et al. (2008) Induction of CYP4F3 by Benzene Metabolites in Human White Blood Cells in Vivo Promeylocytic Leukemic Cell
Lines and ex Vivo in Human Blood Neutrophils. Drug Metabolism and
Disposition, 37 (2), 282-291.
http://www.allcells.com/publications/0083allcells.pdf
Vose, Sara C., et al. (2007) Lysophosphatidylcholine Hydrolases of
Human Erythrocytes, Lymphocytes and Brain: Sensitive Targets of
Conserved Specificity for Organophosphorus Delayed Neurotoxicants.
Toxicol Appl Pharmacol., 224(1), 98–104.
http://www.allcells.com/publications/0089allcells.pdf
Duramad, Paurene, et al. (2006). Expression of Th1/Th2 cytokines in
human blood after in vitro treatment with chlorpyrifos and its metabolites, in combination with endotoxin LPS and allergen Der p1. Journal
of Applied Toxicology, 26(5), 458-465.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
38
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB017
10 ml
$104
PB018
20 ml
$146
PB019
30 ml
$181
PB020
40 ml
$216
PB021
50 ml
$240
PB022
100 ml
$303
PB023
150 ml
$361
PB017F
10 ml
$81
PB018F
20 ml
$123
PB019F
30 ml
$158
PB020F
40 ml
$193
PB021F
50 ml
$216
PB022F
100 ml
$280
PB023F
150 ml
$338
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Plasma
Lead Time
Product Description
NPB Plasma is obtained from healthy donors through the
COBE Spectra™ apheresis machine. Between 50 and 150
mL of autologous plasma is collected at the same time as
the mononuclear cells.
Application
NPB-Plasma can be used to:
• Analyze blood plasma components.
• Study plasma transfusion efficiency for coagulopath
and liver diseases.
• Function as source of serum if platelets, fibrinogen, and
other coagulating factors are removed.
• Serve as a control in studies on Factor VIII and
coagulopathies, such as hemophilia.
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of one week is needed.
Related Products
ALL Plasma
AML Plasma
CB Plasma
Published Papers
Francisco, Joseph A. et al. (2003). cAC10-vcMMAE, an anti-CD30–
monomethyl auristatin E conjugate with potent and selective
antitumor activity. Blood, 102(4), 1458-1465.
http://www.allcells.com/publications/0050allcells.pdf
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Plasma
39
Normal Peripheral Blood
Normal Peripheral Blood—Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB001
150 million/vial
$414
PB002
350 million/vial
$567
PB003F
15 million/vial
$118
PB006F
25 million/vial
$173
PB004F
50 million/vial
$244
PB005F
100 million/vial
$380
Comprehensive RNA
RNA-PB001C
50.0 µg
$410
cDNA
cDNA-PB001
30 PCR rxns
$294
Frozen
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Table: NPB Cell Subset Products
NPB-MNC
Product Description
Mononuclear cells are collected from fresh blood during
apheresis into Leuko Paks which enriches lymphocytes
and monocytes while depleting mature granulocytes. The
mononuclear fraction is then further processed to eliminate red
blood cells and platelets.
NPB Cell Subsets
Average Percentage in MNC
CD34+
0.2%
CD3+
60%
CD4
30%
+
CD8+
23%
CD14
15%
CD19+
9%
CD56
7%
Basophils
0.3%
Neutrophils and Eosinophils
0.2%
RBC
<0.01%
Platelets
<0.01%
+
+
Application
NPB-MNC can be used to:
• Isolate and study MNC subpopulations.
• Act as controls for therapeutic research, involving
lymphocytes and monocytes.
• Study the relationship among MNC subpopulation
(for example, the relationship among T Cells, B Cells, and
monocytes)
• Research anti-GVHD drugs.
Lead Time
A minimum of two weeks is needed for fresh cells.
The table above is for reference purposes only. Please note that the average
percentage of cell subsets is referring to the recovered MNC population and not
the total white blood cell population. We cannot guarantee the recovery of purifica-
tion products listed above. For a specific number of a certain cell type, please consider
our normal peripheral blood purified products.
Related Products
MPB-MNC
BM-MNC
CB-MNC
FL-MNC
Published Papers
Arastu-Kapur, Shirin, et al. (2011) Nonproteasomal Targets of the Proteasome Inhibitors Bortezomib and Carfilzomib: a Link to Clinical Adverse
Events. Clinical Cancer Research, 17, 2734.
http://www.allcells.com/publications/0160allcells.pdf
Xu, Yuanxin, et al. (2008) Validation of a HLA-A2 tetramer flow cytometric
method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma
patients. Journal of Translational Medicine, 6(61).
http://www.allcells.com/publications/0033allcells.pdf
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase (PTPN22) Is
Associated with Rheumatoid Arthritis. Am. J. Hum. Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
40
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB032-1
0.5 million/vial
$1994
PB032-2
1 million/vial
$3485
PB033F-1
0.2 million/vial
$805
PB033F-2
0.5 million/vial
$1928
PB033F-3
1 million/vial
$3104
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD34+ Stem/Progenitor Cells
Product Description
NPB-CD34+ stem/progenitor cells (NPB-CD34+) are
positively isolated from mononuclear cells using an
immunomagnetic CD34 MicroBead labeling system.
Application
NPB-CD34 cells can be used to:
• Study expansion, lineage generation, and transplantation
potentials.
• Serve as an alternative, less invasive blood source, to bone
marrow.
+
Note: NPB-CD34+ cells require more source volume due
to low percentages in peripheral blood.
Related Products
BM-CD34+ Cells CB-CD34+ Cells FL-CD34 + Cells MPB-CD34+ Cells
Published Papers
Mack, Amanda A., et al. (2011) Generation of Induced Pluripotent
Stem Cells from CD34+ Cells across Blood Drawn from Multiple Donors with Non-Integrating Episomal Vectors. PLoS ONE, 6(11).
http://www.allcells.com/publications/0138allcells.pdf
Iancu-Rubin, Camilia, et al. (2011) Down-regulation of stathmin
expression is required for megakaryocyte maturation and platelet
production. Blood, 117(17).
http://www.allcells.com/publications/0149allcells.pdf
Dongjiu, Ye, et al. (2009) Analysis of Aurora kinase A expression in
CD34+ blast cells isolated from patients with myelodysplastic syndromes and acute myeloid leukemia. J Hematopathol, 2, 2-8.
http://www.allcells.com/publications/0034.pdf
Wang, Lisheng, et al. (2007) Pharmacologic inhibition of CDK4/6:
mechanistic evidence for selective activity or acquired resistance in
acute myeloid leukemia. Blood, 110(6).
http://www.allcells.com/publications/0096allcells.pdf
Purity
The purity is guranteed to be at least 85% as assessed by flow
cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Stem/Progenitor Cells—CD34 + Cells
41
Normal Peripheral Blood—B Cells—CD19 + B Cells
Normal Peripheral Blood
Untouched Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB010
10 million/vial
$963
Frozen
PB010F
10 million/vial
$799
Fresh
PB010-P
10 million/vial
$779
Frozen
PB010-P-F
10 million/vial
$703
Comprehensive RNA
RNA-PB003C
3.0 µg
$418
cDNA
cDNA-PB003
10 PCR rxns
$436
Positively Selected Cell Products
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD19+ B Cells
Product Description
Purity
Untouched NPB-CD19+ B Cells are negatively isolated from
mononuclear cells using an indirect immunomagnetic labeling
system.
Positively Selected NPB-CD19+ B Cells are directly isolated
from mononuclear cells using immunomagnetic CD19
MicroBeads.
Untouched NPB-CD19+ B Cells: The purity is guaranteed to be
at least 85% as assessed by flow cytometric analysis.
Positively Selected NPB-CD19+ B Cells: The purity is
guaranteed to be at least 90% as assessed by flow cytometric
analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-B Cells can be used to research:
• Cell-mediated immunity pathway
• B cell activation and cloning mechanisms
• Genetic rearrangements of immunoglobulins
• Antigen presentation
• Recognition mechanisms
• Cloning of memory and plasma cells
• Antibody and immunoglobulin secretion
• Toll-like receptors
Related Products
CB-B Cells MPB-B Cells
Published Papers
Akers, Stephen M., et al. (2010) VE-cadherin and PECAM-1 enhance ALL
migration across brain microvascular endothelial cell monolayers. ISEH-Society for Hematology and Stem Cells, doi: 10.1016/j.exphem.2010.05.001.
Mahadevan, Daruku, et al. (2009) Gene Expression and Serum Cytokine
Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3, and CXCL9/
CXCR3 as Regulators of Cell Proliferation, Survival, and Migration. SAGEHindawi Access to Research Human Genomics and Proteomics, 2009.
http://www.allcells.com/publications/0064allcells.pdf
Baysa, Bora E., et al. (2007) A Recurrent Stop-Codon Mutation in Succinate Dehydrogenase Subunit B Gene in Normal Peripheral Blood and
Childhood T-Cell Acute Leukemia Bora E. PLoS ONE, 5(e46).
http://www.allcells.com/publications/0012allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
42
Normal Peripheral Blood—B Cells—CD19 +/IgD + Naïve B Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh/Untouched
PB010-3
1 million/vial
$1157
Frozen/Untouched
PB010-3F
1 million/vial
$1127
Comprehensive RNA
RNA-PB020C
1.0 µg
$418
cDNA
cDNA-PB020
10 PCR rxns
$580
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD19+/IgD+ B Cells
Purity
Product Description
NPB-CD19+/IgD+ B cells are negatively isolated
from MNC using immunomagnetic isolation system to
deplete non-naive B cells.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-Memory B Cells can be used to study:
• Cell-mediated immunity pathway
• B cell activation and cloning mechanisms
• Signal transductions
• Antigen uptake and presentation
• Antibody and immunoglobulin secretion
• Cognate interaction of naive B cells with T helper
cells or dentdritic cells
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Cell Products
43
Normal Peripheral Blood—B Cells—CD19 +/CD27+ Memory B Cells
Normal Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh/Untouched
PB010-4
0.5 million/vial
$981
Frozen/Untouched
PB010-4F
0.5 million/vial
$858
Comprehensive RNA
RNA-PB021C
1.0 µg
$418
cDNA
cDNA-PB021
10 PCR rxns
$788
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD19+/CD27+Memory B Cells
Product Description
Purity
NPB-CD19+/CD27+ memory B cells are negatively
isolated from MNC using immunomagnetic isolation
system to deplete non-memory B cells.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-Memory B Cells can be used to study:
• Cell-mediated immunity pathway
• B cell activation and cloning mechanisms
• Signal transductions
• Antigen uptake and presentation
• Antibody and immunoglobulin secretion
• Cloning of memory and plasma cells
• Antibody production and differentiation
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
44
Normal Peripheral Blood—T Cells—CD3 + Pan T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-1
20 million/vial
$779
Frozen
PB009-1F
20 million/vial
$669
Comprehensive RNA
RNA-PB002C
5.0 µg
$410
cDNA
cDNA-PB002
10 PCR rxns
$294
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB- Pan T Cells (CD3+)
Product Description
NPB-Pan T Cells are negatively isolated from mononuclear
cells using an indirect immunomagnetic Pan-T labeling system.
Application
NPB-Pan T Cells can be used to research the following:
• T cell activation
• Maintenance of the naïve T cells (CD45RA+)
• Transformation to memory (CD45RO+) and or regulatory (CD25+) T cells, and their respective functions
• Intercellular T-cell regulatory and activation pathways
• Antigen presentation and other receptor pathways
• Recognition mechanisms
Related Products
CB-Pan T Cells
MPB-Pan T Cells
Published Papers
Yang, Jianchang, et al. (2008) SALL4 is a key regulator of survival and apoptosis in human leukemic cells. Blood. doi: 10.1182/
blood-2007-11-126326.
http://www.allcells.com/publications/0106allcells.pdf
Mahadevan, Daruku, et al. (2005) Transcript profiling in peripheral
T-cell lymphoma, not otherwise specified, and diffuse large B-cell lymphoma identifies distinct tumor profile signatures. Mol Cancer Ther,
4(12). http://www.allcells.com/publications/0112allcells.pdf
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
Purity
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells. Frozen
products are subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Untouched Cell Products
45
Normal Peripheral Blood—T Cells—CD4 + Helper T Cells
Normal Peripheral Blood
Untouched Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-2
15 million/vial
$779
Frozen
PB009-2F
15 million/vial
$671
Comprehensive RNA
RNA-PB007C
5.0 µg
$410
cDNA
cDNA-PB007
10 PCR rxns
$294
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD4+ Helper T Cells
Product Description
NPB CD4+ Helper T cells are negatively isolated from
mononuclear cells using an indirect immunomagnetic CD4 + T
cell labeling system.
Application
NPB-CD4 Helper T Cells can be used to study:
• Effect on B cell antibody class switching
• HIV infection mechanisms and therapeutic research
• Antigenic presentation, intracellular activation
mechanisms, and learned immunity
• Mechanism and therapeutics of hypersensitivity and
autoimmune disease
+
Related Products
CB-CD4+ T Cells
MPB- CD4+ T Cells
Published Papers
Mahadevan, Daruku, et al. (2005) Transcript profiling in peripheral
T-cell lymphoma, not otherwise specified, and diffuse large B-cell lymphoma identifies distinct tumor profile signatures. Mol Cancer Ther,
4(12). http://www.allcells.com/publications/0112allcells.pdf
Lu, Xiaobin, et al. (2004) Antisense-Mediated Inhibition of Human
Immunodeficiency Virus (HIV) Replication by Use of an HIV Type
1-Based Vector Results in Severely Attenuated Mutants Incapable of
Developing Resistance. Journal of Virology, 78(13), 7079-7088.
http://www.allcells.com/publications/0043allcells.pdf
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
Purity
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
46
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-4
2 million/vial
$935
Frozen
PB009-4F
2 million/vial
$818
Comprehensive RNA
RNA-PB015C
1.0 µg
$410
cDNA
cDNA-PB014
10 PCR rxns
$1002
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD4+/CD25+ T Cells
Product Description
Purity
First, CD4+ T Cells are isolated using an indirect
immunomagnetic depletion. Next, CD25+ cells are positively
isolated using CD25bright MicroBeads, leaving a highly purified
CD4+/CD25+ Regulatory T Cell population.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
Application
NPB-CD4+/CD25+ Regulatory T Cells can be used to study:
• Homeostasis in the immune system.
• Hypersensitivity and autoimmunity.
• Methods to improve the body’s defense against infection
and tumors.
• Activated T cells
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—T Cells—CD4 +/CD25 + T Cells
47
Normal Peripheral Blood
Normal Peripheral Blood—T Cells—CD4 +/CD45RA+/CD25 - Naïve Helper T
Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-5
5 million/vial
$981
Frozen
PB009-5F
5 million/vial
$859
Comprehensive RNA
RNA-PB016C
3.0 µg
$418
cDNA
cDNA-PB016
10 PCR rxns
$348
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD4+/CD45RA/CD25-+ Naïve Helper T Cells
Purity
Product Description
First, NPB-CD4+ T Cells are negatively isolated using
immunomagnetic CD4+ isolation kit from mononuclear cells.
Next, CD45RO MicroBeads are used to deplete the CD45RO+
cell population, leaving an untouched, purified CD4+/CD45RA+
Naïve T Cell population.
Application
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
Related Products
CB-CD4+/CD45RA+ T Cells
NPB-CD4+/CD45RA+ Naïve T Cells can be used to study:
• Naïve T cell activation
• Memory T Cell training and activation for long-term
immunity
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
48
Normal Peripheral Blood—T Cells—CD4 +/CD45RO + Memory T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-7
5 million/vial
$981
Frozen
PB009-7F
5 million/vial
$919
Comprehensive RNA
RNA-PB018C
1.0 µg $418
cDNA
cDNA-PB018
10 PCR rxns
$299
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD4+/CD45RO+ Memory T Cells
Purity
Product Description
NPB-CD4+ T Cells are negatively isolated from MNC using an
indirect immunomagnetic CD4 +/CD45RO+ labelling system.
Lead Time
Application
NPB-CD4+/CD45RO+ Memory T Cells can be used to study:
• The capacity for self-renewal (as a result of high levels of
phosphorylation and the transcription factor STAT5).
• Memory T cell development and maintenance specificity
for different antigens.
• Memory T Cell activation mechanisms and pathways
ALLCELLS
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells.
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Cell Products
49
Normal Peripheral Blood—T Cells—CD8 + Cytotoxic T Cells
Normal Peripheral Blood
Untouched Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-3
10 million/vial
$779
Frozen
PB009-3F
10 million/vial
$671
Comprehensive RNA
RNA-PB008C 5.0 µg
$410
cDNA
cDNA-PB008
10 PCR rxns
$294
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD8+ Cytotoxic T Cells
Product Description
NPB-CD8+ Cytotoxic T cells are negatively isolated from MNC
using an indirect immunomagnetic CD8 + T cell labeling system.
Application
NPB-CD8+ T Cells can be used to study:
• VDJ recombination mechanisms of the TcR (T cell
receptor), self/non-self recognition, and autoimmunity
• Cytotoxic cell lysis and platelet activation to infected
tissues or organs
• Defense mechanisms against tumor and viral infections
Related Products
CB-CD8+ T Cells
MPB- CD8+ T Cells
Published Papers
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
Purity
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
50
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-6
5 million/vial
$963
Frozen
PB009-6F
5 million/vial $842
Comprehensive RNA
RNA-PB017C
3.0 µg
$410
cDNA
cDNA-PB017
10 PCR rxns
$489
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD8+/CD45RA+ Naive Cytotoxic T Cells
Product Description
Purity
First, NPB-CD8+ T Cells (CTL) are negatively isolated from
MNC using immunomagnetic CD8+ T cell isolation kit. Next,
CD45RO MicroBeads are used to deplete the CD45RO+ cell
population, leaving a purified CD8+/CD45RA+ Naïve T Cell
population.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
Application
NPB-CD8+/CD45RA+ Naïve Cytotoxic T Cells can be used to
study:
• Homeostasis of Naive CTL in its surrounding repertoire
• Activation of Naïve CTL
• Methods to redirect the CTL’s learning pathway for
autoimmune therapy
• In vitro engineering to fight specific targets, such as
viruses or tumors
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—T Cells—CD8 +/CD45RA+ Naive Cytotoxic T Cells
51
Normal Peripheral Blood
Normal Peripheral Blood—Dendritic Cells—BDCA4 + Lymphoid/
Plasmacytoid Dendritic Cells
Positively Selected Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB013
0.5 million/vial
$842
Frozen
PB013F
0.5 million/vial
$819
RNA-PB006C
0.5 µg
$790
Molecular Products
Comprehensive RNA
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-BDCA4+ Lymphoid/Plasmacytoid Dendritic Cells
Purity
Product Description
NPB-BDCA4 + Lymphoid/Plasmacytoid Dendritic cells have
been positively isolated from mononuclear cells using
immunomagnetic BDCA-4 MicroBead labeling system.
BDCA-4 is marker for CD11c-, CD123bright (lymphoid) dendritic
cells. NPB-BDCA4+ cells possess plasmacytoid morphology
and express the CD45RA cell marker. BDCA-4 is not
detectable on the cell surface of other blood cells.
NPB-BDCA4 + cells can be used to study:
• Antigen presentation
• Activation of toll-like receptors and other pattern
recognition receptors of viral immunity pathways
• Interferon alpha secretion
• Potential uses in anti-tumor research
ALLCELLS
Lead Time
A minimum of two weeks is needed for fresh cells.
Related Products
MPB-BDCA4+ T Cells
Application
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Toll Free Phone: 1-888-535-3444
Published Papers
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
www.allcells.com
For In Vitro Research Use Only
52
Normal Peripheral Blood—CD56 + Natural Killer Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB012
5 million/vial
$928
Frozen
PB012F
5 million/vial
$799
Fresh
PB012-P
5 million/vial
$756
Frozen
PB012-P-F
5 million/vial
$648
Comprehensive RNA
RNA-PB005C
3.0 µg
$419
cDNA
cDNA-PB005
10 PCR rxns
$576
Positively Selected Cell Products
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD56+ Natural Killer Cells
Lead Time
Product Description
Untouched NPB-CD56+ Natural Killer (NK) cells are negatively
selected from mononuclear cells using an indirect immunomagnetic NK cell labeling system to deplete the non-CD56+
NK cells.
Positively Selected NPB-CD56 + NK cells are directly isolated
from mononuclear cells using direct immunomagnetic CD56
MicroBeads.
Application
A minimum of two weeks is needed for fresh cells. For all other
products, product delivery is subject to stock availability.
Related Products
CB Natural Killer Cells
MPB Natural Killer Cells
Published Papers
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
NPB-NK Cells can be used to study:
• Innate immune system against tumors and viral infections
• Inhibitory receptor recognition and activation
• Cytolytic mechanisms
• Antibody dependent cellular cytotoxicity assay (ADCC)
• FcR binding affinity
• Therapeutic antibody development
Purity
Untouched NPB-CD56+ Cells: The purity is guaranteed to be
at least 85% as assessed by flow cytometric analysis.
Positively Selected NPB-CD56+ Cells: The purity is guaranteed
to be at least 90% as assessed by flow cytometric analysis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Untouched Cell Products
53
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Basophils
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB014
0.3 million/vial
$963
Frozen
PB014F
0.3 million/vial
$842
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Basophils
Product Description
Purity
NPB-Basophils are isolated from peripheral blood MNC using
an indirect immunomagnetic basophil labeling system.
Lead Time
Application
NPB-Basophils can be to study the following:
• Inflammatory responses, involving mast cell differentiation,
IgE expression, and histamine release. The study of these
responses and pathways can lead to remedies for allergy
and asthma.
• Anti-allergy remedies, such as transient transfection
of basophils (inhibiting IgE expression) and other
extracellular methods of basophil suppression.
ALLCELLS
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells.
Related Products
BM-Basophils
CB-Basophils
www.allcells.com
For In Vitro Research Use Only
54
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB015
1 million/vial
$963
Frozen
PB015F
1 million/vial
$842
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Eosinophils
Product Description
Purity
RBC depleted leukocytes are collected from peripheral blood
by sedimentation of red blood cells using HetaSep. Then, the
granulocytes are isolated by density gradient separation and
further processed to deplete any remaining RBC. Finally, the
eosinophils are isolated using an indirect immunomagnetic
labeling system.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of one week is needed for fresh cells.
Application
NPB-Eosinophils can be used to study:
• Post-pubertal mammary gland development
• Estrous cycles
• Allograft rejection
• Neoplasia
• Antigenic presentation to T cells
• Treatment and prevention for atopic dermatitis
• Degranulation and release mechanisms for peroxidases,
nucleases, plasminogens, and major basic proteins
• Cytokine and interleukin release by activation
ALLCELLS
Toll Free Phone: 1-888-535-3444
Related Products
BM-Eosinophils
CB-Eosinophils
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Eosinophils
55
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Neutrophils
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB016
40 million/vial
$842
Comprehensive RNA
RNA-PB011C
1.0 µg
$705
cDNA
cDNA-PB011
10 PCR rxns
$563
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Neutrophils
Purity
Product Description
RBC depleted leukocytes are collected from peripheral blood
by sedimentation of red blood cells using HetaSep. Then, the
granulocytes are isolated by density gradient separation and
further processed to deplete any remaining RBC. Finally, the
neutrophils are isolated using an indirect immunomagnetic
labeling system.
Application
NPB-Neutrophils can be used to study:
• Acute inflammation and tissue injury response
• Degranulation mechanisms.
• Function of neutrophils to form NET (neutrophil extracellular traps) that act as a bacterial killing pathway
independent of phagocytosis.
• Neutropenia (naturally occurring or chemotherapy
induced).
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of one week is needed for fresh cells.
Related Products
BM-Neutrophils
CB-Neutrophils
Published Papers
Zhao, Zhiwei, et al. (2008) Induction of CYP4F3 by Benzene Metabolites in Human White Blood Cells in Vivo Promeylocytic Leukemic Cell
Lines and ex Vivo in Human Blood Neutrophils. Drug Metabolism and
Disposition, 37 (2), 282-291.
http://www.allcells.com/publications/0083allcells.pdf
Brogdon, Jennifer, et al. (2007). Histone deacetylase activities are
required for innate immune cell control of Th1 but not Th2 effector
cell function. Blood, 109(3), 1123-1130.
www.allcells.com
For In Vitro Research Use Only
56
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB029
80 billion/vial
$422
PB030
160 billion/vial
$527
PB031
240 billion/vial
$762
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Mature Erythrocytes / Red Blood Cells
Purity
Product Description
NPB Mature Erythrocytes, also referred to as red blood cells
(RBC), are obtained from normal peripheral blood using size
and density separation methods.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of one week is needed.
NPB-RBC can be used to study anemia caused by
(but not limited to):
• Sickle cell disease
• Thalassemia
• Spherocytosis
• Pernicious anemia
• Aplastic anemia
• Pure red blood cell aplasia
• Hemolysis
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Red Blood Cells / Mature Erythrocytes
57
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Platelets
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB025
5 billion/vial
$181
PB026
10 billion/vial
$303
PB027
20 billion/vial
$422
PB028
30 billion/vial
$544
Comprehensive RNA
RNA-PB014C
0.1 µg
$410
cDNA
cDNA-PB015
10 PCR rxns
$410
Molecular Products
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Platelets
Product Description
Lead Time
NPB-Platelets are obtained from healthy donors with the
collection of maximum three-blood volumes on a COBE
Spectra™ apheresis machine. The platelets are shipped in
platelet-rich plasma.
Application
NPB platelets can be used to study the following:
• Aggregation and coagulation mechanisms
and pathways
• Atherosclerosis
• Coronary artery disease
• Myocardial infarction
• Cerebrovascular diseases
• Stroke
• Peripheral artery occlusive disease
• Cancer, including prevention and treatment of transfusion
A minimum of two weeks is needed for fresh cells.
Published Papers
Kubota, Satoshi, et al. (2004). Abundant retention and release of
connective tissue growth factor (CTGF/CCN2) by platelets. Journal
of Biochemistry, 136(3), 279-282.
related complications.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
58
Normal Peripheral Blood—Myeloid Monocytes—CD14 + Monocytes
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB011
10 million/vial
$928
Frozen
PB011F
10 million/vial
$799
PB011-P-1
10 million/vial
$779
PB011-P-2
20 million/vial
$1000
PB011-P-3
40 million/vial
$1224
PB011-P-F-1
10 million/vial
$671
PB011-P-F-2
20 million/vial
$875
PB011-P-F-3
40 million/vial
$1113
Comprehensive RNA
RNA-PB004C
5.0 µg
$419
cDNA
cDNA-PB004
10 PCR rxns
$330
Positively Selected Cell Products
Fresh
Frozen
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD14+ Monocytes
Product Description
Purity
Untouched NPB-CD14+ Monocytes are indirectly isolated
from mononuclear cells using an immunomagnetic labeling
system.
Positively-Selected NPB-CD14+ Monocytes are directly
isolated from mononuclear cells using an immunomagnetic
CD14 MicroBead labeling system.
Application
NPB-CD14 Monocytes can be used to study:
• Post-pubertal mammary gland development
• Estrous cycle
• Allograft rejection
• Neoplasia
• Antigenic presentation to T cells
• Treatment and prevention for atopic dermatitis
• Toll-like receptors and other pattern recognition
receptors
+
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 85% for untouched
monocytes, and at least 90% for positively selected
monocytes..
Lead Time
A minimum of two weeks is needed for fresh cells.
Related Products
CB- Monocytes
MPB-Monocytes
Published Papers
Wang, Jinghong, et al. (2006) Kynurenic Acid as a Ligand for Orphan
G Protein-coupled Receptor GPR35. The Journal of Biological Chemistry, doi: 10.1074/jbc.M6035003200.
http://www.allcells.com/publications/0085allcells.pdf
Braselmann, Sylvia, et al. (2006). R406, an Orally Available Spleen
Tyrosine Kinase Inhibitor Blocks Fc Receptor Signaling and Reduces
Immune Complex-Mediated Inflammation. Journal of Pharmacology
and Experimental Therapeutics, 319, 998-1008.
http://www.allcells.com/publications/0101allcells.pdf
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Untouched Cell Products
59
Normal Peripheral Blood
Normal Peripheral Blood—Monocytes—Monocyte-Derived Myeloid Dendritic Cells
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
PB-DC001F
1.5 million/vial
$567
PB-DC002F
10 million/vial
$116
PB-DC003F
50 million/vial
$466
PB-DC004F
100 million/vial
$793
PB-DC005F
200 million/vial
$1399
Comprehensive RNA
RNA-PB013C
1.0 µg
$410
cDNA
cDNA-PB013
10 PCR rxns
$328
MNC from same donor
Frozen
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Monocyte-Derived Myeloid Dendritic Cells
•Properties
Product Description
NPB-Monocyte Derived Myeloid Dendritic Cells (MoDC) are
derived from highly purified monocytes. For six to seven days,
the highly purified monocytes are cultured with 10% Fetal
Bovine Serum in the presence of GM-CSF and IL-4. MoDC
have a longer lifespan in vitro when kept inactivated, which
allows them to have a longer storage in culture.
Application
These cells express the following markers: CD11c, CD40,
and CD86 (>90%). These cells lack the expression of CD14
(<10%).
Lead Time
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Monocyte Derived Myeloid DC can be used to study:
• Antigenic endocytosis, processing, and presentation
mechanism and pathway
• DC-SIGN receptor expression and its relation to HIV
infection
• Toll-like receptors and other pattern recognition receptors
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
60
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
PB-MDM-001F
1.5 million/vial
$601
Comprehensive RNA
RNA-PB-MDM-001C
1 µg
$455
cDNA
cDNA-PB-MDM-001
10 PCR rxns
$330
Molecular Products
HLA-Typed Products are available upon request.
Media
Macrophage Basal Medium
PB-MDM-002
450 ml
$59
Macrophage Stimulatory
Supplement
PB-MDM-003
50 ml
$330
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Monocyte-Derived Macrophages
Product Description
Product Description
NPB-Macrophages are derived from negatively-selected
peripheral blood monocytes and stimulated with M-CSF
and IL-4 growth factors and cultured for 6-7 days.
Application
Monocyte-Derived Macrophages can be used to study
the following:
• Inflammation
• Antigen presentation
• T-Cell activation
• Phagocytosis pathways
• Atherosclerosis
• HIV infection
• Toll-like receptors and other pattern recognition
receptors
These cells have phagocytic properties and express the
following markers: MHC class II (>90%), CD11b (>90%),
CD18 (>90%), CD32 (>90%), and CD68 (>90%).
ALLCELLS
This product contains L-glutamine, 110mg/L sodium pyruvate,
and pyridoxine hydrochloride. This product does not contain
growth factors and antibiotics. pH >7.1 to <7.5. The Macrophage Basal Medium should be used in combination with
AllCells Macrophage Stimulatory Supplement. This product
is stable for one month after the stimulatory supplement has
been added and stored at 2 to 8°C.
Macrophage Stimulatory Supplement
Product Description
Macrophage Stimulatory Supplement is designed to initiate and
maintain growth of AllCells Monocyte Derived Macrophages.
This product contains pre-selected Fetal Bovine Serum and
supplements.
Lead Time
Properties
Macrophage Basal Medium
Toll Free Phone: 1-888-535-3444
1-2 business days for frozen products. Product delivery is subject to stock availability.
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Monocyte-Derived Macrophages
61
Mobilized Peripheral Blood
Mobilized Peripheral Blood Table of Contents
Apheresis Collection (Leuko Pak)
62
Stem/Progenitor Cells - CD133+ 66
Mononuclear Cells (MNC)
63
Monocytes - CD14+67
CD34 Depleted Mononuclear Cells
64
Natural Killer Cells - CD56+68
Stem/Progenitor Cells - CD34+ 65
Mobilized Peripheral Blood
AllCells has been a pioneer in the use of G-CSF (Granulocyte-Colony Stimulating Factor) stimulation in normal healthy donors since
1998. The protocols in use for our Mobilized Peripheral Blood (MPB) products have been approved by an IRB and are overseen by clinical
physicians. In addition all donors are monitored during the injection, collection procedure and post-collection to ensure the health of our
donors is a priority. AllCells is one of only a few providers of Mobilized Peripheral Blood in the world.
In addition AllCells now offers Plerixafor (Mozobil®) mobilization of stem cells from healthy donors. Mozobil, a selective CXCR4 antagonist, has
been particularly beneficial as an adjuvant stem cell mobilization agent on top of the G-CSF regimen. The Mozobil regimen requires only 1
injection as opposed to the G-CSF 3-5 day protocol. AllCells is currently the only source of Plerixafor-mobilized cells in the world.
Please specify which mobilization factor you prefer when ordering. Lysate and Cell Pellet products are available upon request.
Background
Granulocyte-Colony Stimulating Factor (G-CSF, Neupogen®) can stimulate bone marrow to produce a large number of hematopoietic
stem/progenitor cells. G-CSF can mobilize these stem/progenitor cells from bone marrow into the peripheral blood stream. The effects
of G-CSF have changed the practice of bone marrow transplantation. Consequently, mobilized peripheral blood cells (MPB) have become
major sources for clinical hematopoietic stem cell transplantation.
Donor Criteria
•
Age 18-40 years old
•
Donors must be in good health
•
Weight > 110 lbs for whole blood >140 lbs for Leuko Pak
•
Donors are required to sign consent form prior to collection
•
Complete Blood Count lab test must meet protocol specs
•
All consent and protocols approved by an IRB
•
Donors cannot be pregnant
•
Minimum 12 months between donations
•
Donors are screened for HIV, Hepatitis B & C
•
Twice-in-a-lifetime maximum donation
For more information visit: www.allcells.com/mobilized-peripheral-blood/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
62
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh−Leuko Pak (One-day)
mPB025-Moz.A
1 Bag
$23671
Fresh−Leuko Pak (One-day)
mPB025-Reg.A
1 Bag
$8912
Fresh−Leuko Pak (One-day)
mPB025-Reg.E
1 Bag
$9897
Fresh−Leuko Pak (Two-day)
mPB026
2 Bags
$13515
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Mobilized Peripheral Blood (MPB)-Leuko Pak
Product Description
One-Day G-CSF MPB-Leuko Pak- This product is obtained
by 5.0-9.6 µg/kg/day of G-CSF injections for 3-5 days and
apheresis on Day 4 or Day 5 (see regimen table below).
•
Mozobil® (Plerixafor) on general profiles and individual MNC phenotypes.
Enrich CD34 + human stem cells for further culture
studies.
Lead Time
A minimum of four weeks is needed.
Leuko Pak Cell Number Ranges
MNC Avg. Cell Number: 2.3x1010 Range: 0.9 to 5.6x1010
CD34+ Avg. Cell Number: 1.86x108
Range: 0.6 to 4.8x108
One-Day Mozobil MPB-Leuko Pak- This product is obtained by a 20 mg/ml, 1.2 ml single dose of Mozobil and apheresis 9-11 hours post injection.
Two-Day G-CSF MPB-Leuko Pak- This product is obtained
by 6.9 µg - 9.0 µg/kg/day (depending on the donor’s weight) of
G-CSF injections for 5 days and apheresis on Day 5 and Day 6
(1 bag delivery per day).
Leuko Pak Cell Number Ranges
MNC Avg. Cell Number: 6.0x1010 Range: 1.2 to 12.4x1010
CD34+ Avg. Cell Number: 4.5x108 Range: 0.9 to 8.1x10 8
Application
MPB-Leuko paks can be used to:
•
Study post-stimulation effects of Neupogen® (G-CSF) or
Related Products
NPB-Leuko Pak
Published Papers
Chy-Anh Tran, et al. (2012). Optimized Processing of Growth Factor
Mobilized Peripheral Blood CD34 Products by Counterflow Centrifugal
Elutriation. Stem Cells Translational Medicine, 1(5) http://www.allcells.
com/publications/0144allcells.pdf
Campbell-Anson, Ryan E., et al. (2008). A new approach for the largescale generation of mature dendritic cells from adherent PBMC using
roller bottle technology. Journal of Immune Based Therapies and Vaccines, 6(1) http://www.allcells.com/publications/0003allcells.pdf
Mahmood, Kutubuddin, et al. (2005). Human cytomegalovirus plasmidbased amplicon vector system for gene therapy. Genetic Vaccines
Therapy, 3, 1. Retrieved June 20, 2008,
http://www.allcells.com/publications/0075allcells.pdf
Hoffman, Joseph F., et al. (2002). Na pump isoforms in human
erythroid progenitor cells and mature erythrocytes. PNAS, 99(22),
4572–14577.
Mahmud, Dolores L., et al. (2002). Phosphorylation of forkhead transcription factors by erythropoietin and stem cell factor prevents acetylation and their interaction with coactivator p300 in erythroid progenitor
cells. Oncogene, 21, 1556–1562.
http://www.allcells.com/publications/0098allcells.pdf
Product
Regimen
mPB025-Moz.A
Mozobil @20 mg/ml, 1.2 ml single dose - Regimen A, apheresis 9-11 hours post injection 1 bag
mPB025-Reg.A
G-CSF @7.5-9.6 µg/kg/day x 3 days - Regimen A, apheresis on day 4
1 bag
mPB025-Reg.E
G-CSF @5 µg/kg/day x 5 days - Regimen E, apheresis on day 5
1 bag
mPB026
G-CSF @6.9-9 µg/kg/day - Regimen C, apheresis on days 5 and 6 (2 bags)
2 bags
ALLCELLS
Toll Free Phone: 1-888-535-3444
Qty
www.allcells.com
For In Vitro Research Use Only
Mobilized Peripheral Blood
Mobilized Peripheral Blood—Apheresis Collection—Leuko Pak
63
Mobilized Peripheral Blood—Mononuclear Cells
Mobilized Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
mPB001
150 million/vial
$655
mPB002
250 million/vial
$786
mPB003
350 million/vial
$917
mPB004F
5 million/vial
$130
mPB005F
15 million/vial
$169
mPB006F
25 million/vial
$261
mPB007F
50 million/vial
$392
mPB008F
100 million/vial
$523
Comprehensive RNA
RNA-mPB001C
50.0 µg
$467
cDNA
cDNA-mPB001
30 PCR rxns
$294
Frozen
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-Mononuclear Cells
Product Description
These mononuclear cells (MNC) are collected using the COBE
Spectra™ apheresis machine (>90% of the cells are MNC).
These MPB-MNC products have been depleted of red blood
cells and platelets.
MPB Cell Subsets
Average Percentage of Cell
Subsets
CD34+
0.8%
CD133+
0.6%
+
CD3
33%
CD4+
18%
CD8
6%
CD14+
25%
CD16+
16%
CD19
5%
CD33+
35%
CD56
6%
CD15+
0.11%
+
Lead Time
A minimum of four weeks is needed for fresh cells. Product
delivery is subject to stock availability.
+
+
Related Products
NPB-MNC
Published Papers
Lund, Troy C., et al. (2008). Granulocyte colony-stimulating factor mobilized CFU-F can be found in the peripheral blood but have limited
expansion potential. haematologica, 93(6)
http://www.allcells.com/publication/0068allcells.pdf
The table above is for reference purposes only. Please note that the average
percentage of cell subsets is referring to the recovered MNC population and not
the total white blood cell population. We cannot guarantee the recovery of purification products listed above. For a specific number of a certain cell type, please
consider our mobilized peripheral blood purified products.
Mahmud, Dolores L., et al. (2002). Phosphorylation of forkhead
transcription factors by erythropoietin and stem cell factor prevents
acetylation and their interaction with coactivator p300 in erythroid
progenitor cells. Oncogene, 21, 1556–1562.
http://www.allcells.com/publications/0098allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
64
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
mPB021F
100 million/vial
$153
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-CD34-Depleted MNC
Product Description
Lead Time
MPB-CD34 Depleted MNC are isolated using
immunomagnetic CD34 MicroBead labeling system to deplete
the MPB-CD34 + cell population. This product contains only a
minimal amount of MPB-CD34+ cells.
A minimum of four weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
BM-CD34 Depleted MNC
CB-CD34 Depleted MNC
Application
MPB-CD34 Depleted MNC can be used to:
• Serve as a cost-effective alternative source of
hematopoietic mononuclear cells, excluding the stem/
progenitor cell population.
• Study the cell population profile changes within the
mononuclear cells after G-CSF simulation.
• Research drugs that suppress GVHD for transfusion
or transplantation purposes.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Mobilized Peripheral Blood
Mobilized Peripheral Blood—CD34 Depleted Mononuclear Cells
65
Mobilized Peripheral Blood
Mobilized Peripheral Blood—Stem/Progenitor Cells—CD34 + Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
mPB009
1 million/vial
$786
mPB010
5 million/vial
$3122
mPB011
10 million/vial
$5234
mPB012
20 million/vial
$7471
mPB013
30 million/vial
$7819
mPB014F
0.2 million/vial
$261
mPB015F
1 million/vial
$655
mPB016F
5 million/vial
$2544
mPB017F
10 million/vial
$3753
mPB018F
20 million/vial
$5164
Comprehensive RNA
RNA-mPB009C
1.0 µg
$467
cDNA
cDNA-mPB009
10 PCR rxns
$1283
Frozen
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-CD34+ Stem/Progenitor Cells
Product Description
MPB-CD34+ Stem/Progenitor cells are positively selected
using a direct immunomagnetic CD34 MicroBead labeling
system.
Purity
Published Papers
Zhu, Xingguo, et al. (2012). NF-Y Recruits Both Transcription Activator and Repressor to Modulate Tissue- and Developmental StageSpecific Expression of Human c-Globin Gene. PLoS ONE, 7(10)
http://www.allcells.com/publications/0172allcells.pdf
MacEneaney, Owen J., et al. (2011). Effects of HIV-1 GP120 and
Protease Inhibitors on Apoptotic Susceptibility of CD34+ Hematopoietic Progenitor Cells. J Acquir Immune Defic Syndr., 56(2), 49–50.
http://www.allcells.com/publications/0151allcells.pdf
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of four weeks is needed for fresh cells.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
66
Mobilized Peripheral Blood—Stem/Progenitor Cells—CD133 + Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
mPB023-1
0.5 million/vial
$786
mPB023-2
2.5 million/vial
$2837
mPB023-3
5 million/vial
$5169
mPB023-4
10 million/vial
$7691
mPB023-5
20 million/vial
$10134
mPB024F-1
0.5 million/vial
$721
mPB024F-2
2.5 million/vial
$2775
mPB024F-3
5 million/vial
$4910
mPB024F-4
10 million/vial
$7295
mPB024F-5
20 million/vial
$10252
Comprehensive RNA
RNA-mPB010C
1.0 µg
$854
cDNA
cDNA-mPB010
10 PCR rxns
$4378
Frozen
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-CD133+ Stem/Progenitor Cells
Purity
Product Description
MPB-CD133+ Stem/Progenitor cells are positively isolated
from MPB-MNC by using a direct immunomagnetic CD133
MicroBeads labeling system. The majority of CD133+ Stem/
Progenitor cells also express the CD34 cell marker.
MPB-CD133+ cells can be used to study expansion and differentiation capacity as nascent stem cells. MPB-CD34+ cells
have the highest frequency of CD133 double-positive cells
when compared to bone marrow and cord blood.
ALLCELLS
Lead Time
A minimum of four weeks is needed. Product delivery is subject
to stock availability.
Application
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Toll Free Phone: 1-888-535-3444
Published Papers
Bissels, Ute, et al. (2011). Combined Characterization of microRNA
and mRNA Profiles Delineates Early Differentiation Pathways of
CD133+ and CD34+ Hematopoietic Stem and Progenitor Cells. STEM
CELLS, 29(5). http://www.allcells.com/publications/0147allcells.pdf
www.allcells.com
For In Vitro Research Use Only
Mobilized Peripheral Blood
Cell Products
67
Mobilized Peripheral Blood—CD14 + Monocytes
Mobilized Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
mPB033-1
15 million/vial
$996
mPB033-2
25 million/vial
$1100
mPB033-3
45 million/vial
$1301
mPB033F-1
15 million/vial
$971
mPB033F-2
25 million/vial
$1045
mPB033F-3
45 million/vial
$1233
Comprehensive RNA
RNA-mPB004C
5.0 µg
$467
cDNA
cDNA-mPB004
10 PCR rxns
$917
Frozen
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-CD14+ Monocytes
Product Description
Purity
MPB-CD14+ Monocytes are positively isolated from MPB-MNC
using a direct immunomagnetic CD14 MicroBeads labeling
system.
Application
MPB-Monocytes can be used to study:
• Down-regulation of T cells for allogeneic transplantation.
• The impact of G-CSF on the antigen-presenting and
macrophage-forming abilities of monocytes.
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of four weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
CB-Monocytes
NPB-Monocytes
www.allcells.com
For In Vitro Research Use Only
68
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
mPB034
5 million/vial
$996
Frozen
mPB034F
5 million/vial
$996
Comprehensive RNA
RNA-mPB005C
5.0 µg
$784
cDNA
cDNA-mPB005
10 PCR rxns
$2015
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-CD56+ Natural Killer Cells
Lead Time
Product Description
MPB-CD56+ Natural Killer (NK) Cells are positively isolated
from mononuclear cells using immunomagnetic CD56 MicroBeads.
A minimum of four weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
CB-NK Cells
NPB-NK Cells
Application
MPB-NK cells can be used to:
• Study the effect of G-CSF-induced NK cell suppression
on GVHD.
• Facilitate ADCC (antibody dependent cellular cytotoxicity)
assays
Purity
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Mobilized Peripheral Blood
Mobilized Peripheral Blood—CD56 + Natural Killer Cells
69
Bone Marrow
Bone Marrow Table of Contents
Aspirates (Whole Bone Marrow)
70
Early Erythroid Progenitor Cells - CD36+
Mononuclear Cells (MNC)
71
Myeloid Cells - CD33 76
CD34 Depleted Mononuclear Cells
72
Endothelial Cells - CD105+77
Stem/Progenitor Cells -CD34 73
+
75
+
Mesenchymal Stem Cells
78
Stem/Progenitor Cells - CD133+ 74
Bone Marrow Cells and Tissue
AllCells is able to provide whole bone marrow aspirate and bone marrow cells collected from healthy individuals. These bone
marrow products are available in fresh or frozen format. Lysate and Cell Pellet products are available upon request.
Background
Bone Marrow (BM) contains hematopoietic stem/progenitor cells, which are self-renewing, proliferating, and differentiating into multi-lineage blood cells. Multipotent, non-hematopoietic stem cells, such as bone marrow mesenchymal stem cells
(MSC), can be isolated from human bone marrow as well. These non-hematopoietic, bone marrow MSCs are capable of both
self-renewal and differentiation into bone, cartilage, muscle, tendons, and fat. 100 mL of bone marrow cells and tissue is
drawn into a 60 cc syringe containing heparin (80 U/mL of BM) from the posterior iliac crest, at a maximum of eight separate
sites. Whole bone marrow products are diluted with PBS.
Donor Criteria
•
Age: 18-55 years old
•
Donors cannot be pregnant.
•
Weight: >110 lbs for 25mL and 50mL donations
•
Donors are screened for HIV, Hepatitis B and Hepatitis C
•
Weight: >130 lbs for 100mL donations
•
CBC lab tests must meet protocol specifications.
•
Donors must be in general good health. Donors with any of the
following will be excluded from donating:
–History of heart, lung, liver, kidney disease (including asthma)
–Blood and bleeding disorders, including sickle cell disease
–Neurologic disorders
before donation.
•
Donors are required to sign a procedure-specific consent form
prior to the collection procedure.
•
All consents and protocols have been approved by an independent biomedical review board.
–Cancer
–Diabetes
–Positive: HIV, HbsAg, HCAb
For more information visit: www.allcells.com/bone-marrow/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
70
Bone Marrow—Aspirates—Bone Marrow (Whole)
Product Format
Catalog #
QTY/Unit
Price/Unit
ABM001-1
3 ml
$243
ABM001
10 ml
$400
ABM002
25 ml
$860
ABM003
50 ml
$1703
ABM004
100 ml
$3099
Comprehensive RNA
RNA-BM001C
15.0 µg
$844
cDNA
cDNA-BM001
30 PCR rxns
$842
Fresh
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-Whole
Amount of White Blood Cells (WBC) in Bone Marrow
Product Description
Bone Marrow (BM) contains hematopoietic stem/progenitor
cells, which have the potential to self-renew, proliferate, and
differentiate into multi-lineage blood cells. Multipotent, nonhematopoietic stem cells, such as mesenchymal stem cells, can
be isolated from human BM as well. These non-hematopoietic,
mesenchymal stem cells are capable of both self-renewal and
differentiation into bone, cartilage, muscle, tendons, and fat.
BM is drawn into a 60cc syringe containing heparin (80 U/mL
of BM) from the posterior iliac crest, 25mL/site, from a maximum of four sites. Whole BM products are diluted with PBS.
Tube Size
Median Number of WBC
Range of WBC
3ml
0.64x108
0.54-0.75x 108
10ml
2.15x108
2.05-2.25x 108
25ml
5.37x10
8
5.28-5.48x 108
50ml
1.08x109
1.07-1.09x 109
100ml
2.15x10
2.14-2.16x 109
9
The above table is for reference purposes only. In bone marrow, mononuclear cells
constitute approximately 20 to 30% of the total white blood cells. AllCells is not
responsible for the MNC or other purified cell recovery. For a specific number of a
certain cell type, please consider our bone marrow purified products.
Application
BM can be used to:
• Serve as a source for hematopoietic progenitor cells, mesenchymal stem cells, and other cell types, such as CD33+
myeloid cells and CD138 + plasma cells.
• Study hematopoietic cell differentiation pathways.
• Act as controls for BM malignancies studies.
Lead Time
Related Products
Diseased Bone Marrow Cells
Published Papers
Oskouei, Behzad N., et al. (2012) Increased Potency of Cardiac
Stem Cells Compared with Bone Marrow Mesenchymal Stem Cells
in Cardiac Repair. Stem Cells Translational Medicine, 1(2) 116124. http://www.allcells.com/publications/0143allcells.pdf
Yamaza, et al. (2010) Immunomodulatory properties of stem cells
from human exfoliated deciduous teeth. Stem Cell Research &
Therapy, 1(5).
http://www.allcells.com/publications/0009allcells.pdf
A minimum of two weeks is needed for fresh cells.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Bone Marrow
Molecular Products
71
Bone Marrow—Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
ABM024
25 million/vial
$315
ABM005
100 million/vial
$664
ABM006
200 million/vial
$1002
ABM007
300 million/vial
$1779
ABM007F
5 million/vial
$123
ABM008F
15 million/vial
$258
ABM009F
25 million/vial
$367
ABM010F
50 million/vial
$561
ABM011F
100 million/vial
$664
Comprehensive RNA
RNA-BM002C
10.0 µg
$467
cDNA
cDNA-BM002
30 PCR rxns
$500
Bone Marrow
Frozen
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-MNC
BM Cell Subset Products
Product Description
BM-MNC are isolated by diluting bone marrow with PBS
and using density gradient separation techniques. After
centrifugation, the MNC layer is collected. MNC can be
processed further to isolate various subpopulations.
The table below is for reference purposes only. Please note that the average
percentage of cell subsets is referring to the recovered MNC population and not
the total white blood cell population.
BM Cell Subsets
Average Percentage of
Cell Subsets
CD34+
2.5%
CD133+
1.1%
CD33
45%
CD105+
0.7%
+
CD19
24%
CD14+
7.8%
Gao, N, et al. (2012) 3, 39-Diindolylmethane Exhibits Antileukemic
Activity In Vitro and In Vivo through a Akt-Dependent Process. PLoS
ONE, 7(2). http://www.allcells.com/publications/0141allcells.pdf
CD3
34%
Granulocytes
10%
Majeti, Ravindra, et al. (2009) Dysregulated gene expression
networks in human acute myelogenous leukemia stem cells. PNAS,
106(9). http://www.allcells.com/publications/0060allcells.pdf
RBC
<0.01%
Published Papers
Novotny-Diermayr, V., et al. (2012) The oral HDAC inhibitor pracinostat (SB939) is efficacious and synergistic with the JAK2 inhibitor
pacritinib (SB1518) in preclinical models of AML. Blood Cancer
Journal, 2, e69. http://www.allcells.com/publications/0159allcells.pdf
+
+
Platelets
<0.01%
CD138 Plasma Cells
0.1-0.4%
+
Seo, BM, et al. (2008) SHED repair critical-size calvarial defects in
mice. Oral Dis. 14(5).
http://www.allcells.com/publications/0107allcells.pdf
We cannot guarantee the recovery of the cell types listed above. For a specific
number of a certain cell type, please consider our bone marrow purified products.
Shuangli, Mi, et al. (2007) MicroRNA expression signatures accurately discriminate acute lymphoblastic leukemia from acute myeloid
leukemia. PNAS, 10.1073.
http://www.allcells.com/publications/0092allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
72
Bone Marrow—CD34 Depleted Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ABM023F
100 million/vial
$315
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-CD34 Depleted MNC
Product Description
Bone Marrow
Related Products
BM-CD34 Depleted MNC are isolated using a direct
immunomagnetic CD34 MicroBead labeling system to deplete
CD34+ cells. This product contains only a minimal amount of
BM-CD34 + cells.
CB CD34-Depleted MNC
Application
BM-CD34 Depleted MNC can be used to enrich and propagate
mesenchymal stem cells.
Lead Time
1-2 business days for frozen products. Product delivery is
subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
73
Bone Marrow—Stem/Progenitor Cells—CD34 + Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
ABM009
0.1 million/vial
$230
ABM010
0.3 million/vial
$392
ABM011
0.5 million/vial
$561
ABM012
1 million/vial
$905
ABM013
5 million/vial
$4472
ABM014
10 million/vial
$8718
Bone Marrow
Frozen
ABM015F
0.1 million/vial
$230
ABM021F
0.3 million/vial
$392
ABM022F
0.5 million/vial
$561
ABM017F
1 million/vial
$899
ABM018F
5 million/vial
$4261
ABM019F
10 million/vial
$8376
Molecular Products
Comprehensive RNA
RNA-BM003C
1.0 µg
$1584
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-CD34+ Stem/Progenitor Cells
Product Description
Lead Time
BM-CD34+ stem/progenitor cells are positively isolated using
a direct immunomagnetic CD34 MicroBead labeling system.
Related Products
CB-CD34+ Cells
MPB-CD34+ Cells
Application
BM-CD34+ cells can be used for the following:
• Study of hematopoiesis and disease pathways.
• Act as a control for leukemia and leukemia stem cell
studies.
• Facilitate colony-forming and stem cell toxicity assays.
• Research in vitro stem cell cloning
Purity
The purity is guaranteed to be at least 90% as
assessed by flow cytometric analysis.
ALLCELLS
A minimum of two weeks is needed for fresh cells.
Toll Free Phone: 1-888-535-3444
FL-CD34 + Cells
NPB-CD34 + Cells
Published Papers
Cokic, Vladan P., et al. (2012) JAK-STAT and AKT pathway-coupled
genes in erythroid progenitor cells through ontogeny. Journal of
Translational Medicine, 10 (116).
http://www.allcells.com/publications/0157allcells.pdf
Karp, Judith E., et al. (2011) Phase 1 and pharmacokinetic study
of bolus-infusion flavopiridol followed by cytosine arabinoside and
mitoxantrone for acute leukemias. Blood, 117 (12).
http://www.allcells.com/publications/0168allcells.pdf
Huang, Xinqiang., et al. (2011) Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem
cells. Blood, 117 (7).
http://www.allcells.com/publications/0167allcells.pdf
Nahimana, A., et al. (2011) A novel potent Fas agonist for selective
depletion of tumor cells in hematopoietic
transplants. Blood Cancer Journal, 1 (e47).
http://www.allcells.com/publications/0150allcells.pdf
www.allcells.com
For In Vitro Research Use Only
74
Bone Marrow—CD133 + Stem/Progenitor Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
ABM027
0.1 million/vial
$371
ABM025
0.5 million/vial
$1196
ABM027F
0.1 million/vial
$370
ABM026F
0.5 million/vial
$1165
RNA-BM004C
0.5 µg
$610
Frozen
Molecular Products
Comprehensive RNA
Bone Marrow
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-CD133+ Stem/Progenitor Cells
Purity
Product Description
Bone Marrow CD133+ Progenitor cells are positively isolated
using a direct immunomagnetic CD133 MicroBead labeling system. The majority of CD133+ cells express the CD34
marker as well.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis
Lead Time
A minimum of two weeks is needed for fresh cells.
Application
BM-CD133+ cells can be used to:
• Research hematopoiesis and disease pathway.
• Serve as a control for leukemia and leukemia stem cells
studies.
• Facilitate colony-forming assays and stem cell toxicity
assays.
• Study stem cell self-renewal.
Related Products
CB CD133+ Cells
MPB-CD133+ Cells
FL-CD133+ Cells
NPB-CD133+ Cells
Published Papers
He, Jia-Qiang, et al. (2011) Human Cardiac Stem Cells Isolated from
Atrial Appendages Stably Express c-kitt. PLoS ONE, 6(11).
http://www.allcells.com/publications/0137allcells.pdf
Chen, Ling, et al. (2007) Identification of CD13+CD36+ cells as a
common progenitor for erythroid and myeloid lineages in human bone
marrow. Exp Hematol, 35(7), 1047-1055.
http://www.allcells.com/publications/0032allcells.pdf
Murmann, Andrea E., et al. (2005) Local gene density predicts the
spatial position of genetic loci in the interphase nucleus. Experimental
Cell Research, 311(1), 14-26.
http://www.allcells.com/publications/0087allcells.pdf
Clark, Rachael A., et al. (2005) Human skin cells support thymusindependent T cell development. The Journal of Clinical Investigation,
115, 3239-3249.
http://www.allcells.com/publications/0006allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
75
Bone Marrow—CD36 + Early Erythroid Progenitor Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ABM020F
1 million/vial
$625
Comprehensive RNA
RNA-BM013C
1.0 µg
$1012
cDNA
cDNA-BM013
10 PCR rxns
$1470
Molecular Products
Bone Marrow
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-CD36+ Early Erythroid Progenitor Cells
Product Description
BM-CD36+ Early Erythroid Progenitor cells are derived from
BM-CD34 + cells in culture. The BM-CD34+ cells are cultured
for 7 to 8 days in serum free media with the addition of SCF,
EPO, IL-3 and IL-6 growth factors. The cells are harvested from
culture, and the BM-CD36+ cells are positively isolated, using
direct immunomagnetic CD36 MicroBeads labeling system.
Application
BM-CD36+ cells can be used to:
• Study the parvovirus B19 tropism for erythroid cells.
• Replace the traditional CD71 + cells as an immediate and
abundant source for erythroid cell studies and transplantation studies.
• Research erythropoiesis.
• Serve as a control in therapeutic studies for early
erythroblastic leukemia and polycythemia vera (PV).
Lead Time
Product delivery is subject to stock availability.
Related Products
CB-CD36+ Cells
FL-CD36+ Cells
MPB-CD36+ Cells
Published Papers
Mulligan, George, et al. (2007). Gene expression profiling and correlation with outcome in clinical trials of the proteasome inhibitor
bortezomib. Blood, 109(8), 3177-3188.
http://www.allcells.com/publications/0065allcells.pdf
Purity
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
76
Bone Marrow—CD33 + Myeloid Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
ABM032-2
1 million/vial
$843
Frozen
ABM032F-2
1 million/vial
$843
Comprehensive RNA
RNA-BM006C
5.0 µg
$444
cDNA
cDNA-BM006
10 PCR rxns
$280
Molecular Products
Bone Marrow
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-CD33+ Myeloid Cells
Product Description
Lead Time
BM-CD33+ myeloid cells are positively isolated from BM-MNC
using a direct immunomagnetic CD33 MicroBead labeling
system.
A minimum of two weeks is needed for fresh cells.
Related Products
CML-BM Cells
Application
BM-CD33+ myeloid cells can be used to:
• Act as a target for transplantation to restore granulocytes
in leukemia patients.
• Study neutropenia.
• Serve as a potential test for anti-leukemia drug candidate
for CML.
Published Papers
Murmann, Andrea E., et al. (2005). Local gene density predicts the
spatial position of genetic loci in the interphase nucleus. Experimental
Cell Research, 311(1), 14-26.
http://www.allcells.com/publications/0087allcells.pdf
Welm, A.L., et al. (2002). C/EBP(alpha) is required for proteolytic
cleavage of cyclin A by calpain 3 in myeloid precursor cells. Journal of
Biological Chemistry.
http://www.allcells.com/publications/0049allcells.pdf
Purity
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
77
Bone Marrow—CD105 + Endothelial Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
ABM028
0.3 million/vial
$875
Frozen
ABM028F
0.3 million/vial
$875
RNA-BM005C
1.0 µg
$1517
Molecular Products
Comprehensive RNA
Bone Marrow
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-CD105+ Endothelial Cells
Product Description
Purity
BM-CD105 Endothelial Cells are positively selected from
bone marrow MNC, using a direct immunomagnetic CD105
MicroBeads labeling system.
+
BM-CD105+ Endothelial cells express endoglin, a type III
receptor of the transforming growth factor-beta (TGF-ß)
receptor family. These cells have the ability for multiplelineage differentiation. After 14 days of culture with
Alpha-MEM + 10% FBS + 1% antimycotic-antibotic, there is
a four-fold expansion of BM-CD105+ Endothelial cells. These
cultured cells express the following cell markers: CD105,
CD13, CD29, CD44, CD49b, CD49e, CD54, CD71, CD90. The
aforementioned markers are similar to the markers expressed
on BM mesenchymal stem cells.
The purity is guaranteed to be at least 80% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
Application
BM-CD105+ Endothelial Cells can be used to study:
• Angiogenesis
• Anti-angiogenesis in tumor therapy research
• Selective multi-potency
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
78
Bone Marrow—Mesenchymal Stem Cells
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MSC-001F
0.75 million/vial
$733
Frozen - Mixed
MSC-001F-M
0.75 million/vial
$667
Comprehensive RNA
RNA-BM011C
3.0 µg
$467
cDNA
cDNA-BM012
10 PCR rxns
$539
HLA-Typed Products are available upon request.
Media
MSC Basal Medium
MSC-002
500 ml
$72
MSC Stimulatory Supplement
MSC-003
50 ml
$243
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-Mesenchymal Stem Cells
Product Description
MSC Basal Medium
Product Description
Bone Marrow Mesenchymal Stem Cells (MSC) are derived
from BM-MNC from the first culture passage.
Application
BM-MSC can be used to:
• Study non-hematopoietic pluripotency potentials and
characteristics to develop connective tissues, such as
bone and cartilage.
• Act as stroma and feeder cells for expansion or
differentiation of hematopoietic stem and progenitor cells.
Mesenchymal Stem Cell Basal Medium is designed to support
AllCells BM-MSC. MSC Basal medium contains L-glutamine,
110mg/L sodium pyruvate, and pyridoxine hydrochloride. This
product does not contain growth factors and antibiotics. The
pH range is >7.1 to <7.5. MSC Basal Medium should be used
in combination with AllCells MSC Stimulatory Supplement.
This product is stable for one month after the stimulatory
supplement has been added and stored at 2 to 8°C.
MSC Stimulatory Supplement
Product Description
Properties
These BM-MSC have fibroblast-like morphology and express
CD105+ (>90%) and CD90+ (>90%). MSC lack expression of
CD34 and CD45.
Lead Time
Mesenchymal Stem Cell Stimulatory Supplement is designed to
initiate and maintain growth of AllCells BM-MSC. This product
contains pre-selected Fetal Bovine Serum and supplements.
Related Products
FL-MSC
1-2 business days for frozen products. Product delivery is subject to stock availability.
Published Papers
Fukushima, H., et al. (2012) SSEA-4 is a Marker of Human Deciduous
Periodontal Ligament Stem Cells. Journal of Dental Research, August
14, 2012. http://www.allcells.com/publications/0152allcells.pdf
Milanesi, Anna, et al. (2012) b-Cell Regeneration Mediated by Human
Bone Marrow Mesenchymal Stem Cells. PLoS ONE, 7(8).
http://www.allcells.com/publications/0142allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Bone Marrow
Molecular Products
79
Cord Blood
Cord Blood Table of Contents
Plasma80
CD14+ Monocytes88
Mononuclear Cells (MNC)81
CD19+ B Cells89
CD34 Depleted Mononuclear Cells
82
CD3+ Pan T Cells90
CD133 Depleted Mononuclear Cells
83
CD4+ Helper T Cells91
Stem/Progenitor Cells - CD34 84
CD8+ Cytotoxic T Cells
92
Stem/Progenitor Cells - CD133+85
CD4+/CD45RA+ Naive T Cells
93
Early Erythroid Progenitor Cells - CD36+86
CD56+ Natural Killer Cells
94
Neutrophils87
Human Umbilical Vascular Endothelial Cells (HUVEC)
95
+
Cord Blood Cells & Tissue
AllCells is able to provide cord blood cells from healthy individuals. These include umbilical cord stem cells, cord blood mononuclear cells,
and additional products. These cord blood products are available in fresh, frozen, lysate, or cell pellet format. AllCells is able to extract
molecular products including Comprehensive RNA and cDNA for your research needs.
Cells can be pulled from mixed or single donors. When ordering please specify preference.
Lysate and Cell Pellet products are available upon request.
Donor Criteria
•
Donors are at least 18 years old.
•
Donors are required to sign a procedure-specific consent form prior to the collection procedure.
•
All consents and protocols have been approved by an independent biomedical review board.
Background
Cord blood (CB) is collected at the time of delivery in a 250 mL standard blood bag containing citrate-phosphate dextrose (CPD) anticoagulant (35mL/bag). The cord blood is processed within 24 hours of collection.
For more information visit: www.allcells.com/cord-blood/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
80
Cord Blood—Plasma
Fresh
Frozen
Catalog #
QTY/Unit
Price/Unit
CB027
10 ml
$103
CB028
20 ml
$145
CB029
30 ml
$179
CB030
40 ml
$214
CB031
50 ml
$239
CB027F
10 ml
$80
CB028F
20 ml
$122
CB029F
30 ml
$156
CB030F
40 ml
$205
CB031F
50 ml
$214
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-Plasma
Product Description
Lead Time
CB-Plasma is obtained by centrifugation of whole cord blood
tissue. This product has a diminished number of platelets.
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Application
Related Products
NPB Plasma
CB-Plasma can be used to study the following:
• Neonatal platelets, cytokines, and immunoglobulins.
• Free-radical trapping ability.
• Beneficial effects of cord blood plasma for cord blood
stem cell transplantation and increased efficiency
during gene transfection.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Cord Blood
Product Format
81
Cord Blood—Mononuclear Cells (MNC)
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB001
150 million/vial
$680
CB002
250 million/vial
$942
CB002F
15 million/vial
$126
CB004F
50 million/vial
$428
CB003F
150 million/vial
$680
Comprehensive RNA
RNA-CB001C
30.0 µg
$407
cDNA
cDNA-CB001
30 PCR rxns
$410
Frozen
Molecular Products
Cord Blood
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-MNC
Cell Subsets in CB
Average Percentage of
(Recoverable) Cell Subsets
CD34+
2.5%
CD133+
4.1%
CD19
45%
CD14+
6%
+
CD3
0.7%
CD4+
24%
CD8
7.8%
Product Description
CB-Mononuclear Cells (MNC) are isolated by diluting the CB
with phosphate-buffered saline and using density gradient
separation techniques. After centrifugation, the MNC layer is
collected. MNC can be processed to isolate subpopulations.
Application
+
+
CB-MNC can be used to study:
• Mesenchymal stem/progenitor cell expansion potential
• Stimulation of neuro-regeneration in the hippocampus
• Sources of endothelial progenitor cells and
neovascularization
• Possible transplantation sources for HIV or SCID diseases
• Remedies for nuclear and radioactive damage
Lead Time
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
CD56+
34%
CD4 /CD45RA
+
+
11%
Granulocytes
10%
RBC
<0.01%
Platelets
<0.01%
The table on the top is for reference purposes only. Please note that the average
percentage of cell subsets is referring to the recovered MNC population and
not the total white blood cell population. We cannot guarantee the recovery of
purification products listed above. For a specific number of a certain cell type,
please consider our cord blood purified products.
Related Products
BM-MNC
FL-MNC
MPB-MNC
NPB-MNC
Published Papers
Medina, Reinhold J, et al. (2010) Molecular analysis of endothelial
progenitor cell (EPC) subtypes reveals two distinct cell populations
with different identities. BMC Medical Genomics, 3 (18).
http://www.allcells.com/publications/0010allcells.pdf
Yang, Li, et al. (2008) IL-21 and TGF-B are required for differentiation
of human TH17 cells. Nature, 454(7202).
http://www.allcells.com/publications/0078allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
82
Cord Blood—CD34 Depleted Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh (Single)
CB011
100 million/vial
$323
Frozen (Single)
CB011F
100 million/vial
$323
Frozen (Mixed)
CB011F-M
100 million/vial
$323
Comprehensive RNA
RNA-CB015C
30.0 µg
$390
cDNA
cDNA-CB015
30 PCR rxns
$399
Molecular Products
HLA-Typed Products are available upon request.
Cord Blood
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD34 Depleted MNC
Product Description
CB CD34 Depleted MNC are isolated, using a direct
immunomagnetic CD34 MicroBead labeling system to deplete
the CD34+ cells. This product contains only a minimal amount
of CD34 + cells.
Related Products
CB-MNC
CB-CD34+ Cells
CB-CD34 Depleted MNC/Mixed indicates that multiple donors
are pooled to obtain the cord blood mononuclear cells.
CB-CD34 Depleted MNC/Single indicates that only one donor
is used to obtain the cord blood mononuclear cells.
Application
CB-CD34 Depleted MNC can be used to research endothelial
cell and mesenchymal stem cell development.
Lead Time
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
83
Cord Blood—CD133-Depleted Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CB014F
100 million/vial
$352
Comprehensive RNA
RNA-CB016C
30.0 µg
$410
cDNA
cDNA-CB016
30 PCR rxns
$410
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD133-Depleted MNC
Cord Blood
Product Description
Lead Time
CB-CD133-Depleted MNC are isolated by using a direct
immunomagnetic CD133 MicroBead labeling system to deplete
the CD133+ cells. This product contains only a minimal amount
of CB-CD133 + cells.
1-2 business days for frozen products. Product delivery is subject to stock availability.
Related Products
CB-MNC
CB-CD133+ Cells
Application
CB-CD133-Depleted MNC can be used to study:
• Mesenchymal stem cell development
• Transplantation and graft-rejection
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
84
Cord Blood—Stem/Progenitor Cells—CD34 + Cells
Mixed Donors/Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB005
1 million/vial
$1100
CB006
5 million/vial
$4344
CB007F
0.2 million/vial
$412
CB005F
0.5 million/vial
$714
CB008F
1 million/vial
$1079
CB009F
5 million/vial
$3821
Fresh
CB005-S
1 million/vial
$1227
Frozen
CB007F-S
0.2 million/vial
$547
CB005F-S
0.5 million/vial
$919
CB008F-S
1 million/vial
$1227
Frozen
Cord Blood
Single Donor/Cell Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD34+ Stem/Progenitor Cells
Product Description
CB-CD34+ Stem/Progenitor cells are positively isolated using
a direct immunomagnetic CD34 MicroBead labeling system.
CB-CD34 + Cells/Mixed indicates that multiple donors are
pooled to obtain the cord blood CD34 + cells.
CB-CD34 Cells/Single indicates that only one donor is used
to obtain the cord blood CD34+ cells.
+
Related Products
FL-CD34+
NPB-CD34 +
BMCD34+
MPBCD34+
Published Papers
Cokic, Vladan P., et al. (2012) JAK-STAT and AKT pathway-coupled
genes in erythroid progenitor cells through ontogeny. Journal of
Translational Medicine, 10 (116).
http://www.allcells.com/publications/0157allcells.pdf
Park, Tea Soon, et al. (2012) Growth Factor-Activated Stem Cell
Circuits and Stromal Signals Cooperatively Accelerate Non-Integrated
iPSC Reprogramming of Human Myeloid Progenitorss. PLoS ONE
7(8). http://www.allcells.com/publications/0128allcells.pdf
Liu, Zhi-Jian, et al. (2011) Developmental differences in megakaryocytopoiesis are associated with up-regulated TPO signaling through
mTOR and elevated GATA-1 levels in neonatal megakaryocytes. Blood
117(15). http://www.allcells.com/publications/0153allcells.pdf
Application
CB-CD34+ cells can be used to study the following:
• Hematopoiesis
• Stem cell expansion and self-renewal
• Transplantation research for hematopoietic malignancies
Cheng, Linzhao, et al. (2011) Efficient human iPS cell derivation by a
non-integrating plasmid from blood cells with unique epigenetic and
gene expression signatures. Cell Research, doi: 10.1038/cr.201.1.12.
http://www.allcells.com/publications/0121allcells.pdf
Purity
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
85
Cord Blood—CD133 + Stem/Progenitor Cells
Mixed Donors/Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB012
0.5 million/vial
$1266
Frozen
CB013F
0.5 million/vial
$980
Fresh
CB012-S
0.5 million/vial
$2651
Frozen
CB013F-S
0.5 million/vial
$2651
Single Donor/Cell Products
HLA-Typed Products are available upon request.
Cord Blood
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD133+ Stem/Progenitor Cells
Purity
Product Description
CB-CD133+ Stem/Progenitor cells are positively isolated using
a direct immunomagnetic CD133 MicroBead labeling system.
The majority of CD133+ stem/progenitor cells express the
CD34 cell marker as well.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
CB-CD133+ Cells/Mixed indicates that multiple donors are
used to obtain the cord blood CD133+ cells.
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
CB-CD133+ Cells/Single indicates that only one donor is used
to obtain the cord blood CD133+ cells.
Application
CB-CD133+ cells can be used to study the following:
• Hematopoiesis
• Stem cell expansion
• Neuro-regeneration
• Transplantation for hematopoietic malignancies
• Effects of nuclear and radioactive damage.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Lead Time
Related Products
BM-CD133+
MPB-CD133 +
Published Papers
Giovanni, C. De, et al. (2012) Human responses against HER-2positive cancer cells in human immune system-engrafted mice. British
Journal ofCancer, doi: 10.1038/bjc.2012.394.
http://www.allcells.com/publications/0155allcells.pdf
Drake, Adam C, et al. (2011) Human CD34+ CD133+ Hematopoietic
Stem Cells Cultured with Growth Factors Including Angptl5
Efficiently Engraft Adult NOD-SCID Il2rc2/2 (NSG) Mice. PLoS ONE,
6 (4). http://www.allcells.com/publications/0132allcells.pdf
www.allcells.com
For In Vitro Research Use Only
86
Cord Blood—CD36 + Early Erythroid Progenitor Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CB010M-F
1 million/vial
$643
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD36+ Early Erythroid Progenitor Cells
Purity
CB-CD36+ Early Erythroid Progenitor cells are derived from
CB-CD34 + cells in culture. CB-CD34+ cells are cultured
for seven to eight days using StemSpan SFEM (StemCell
Technologies, Inc.), a serum free expansion media for
hematopoietic cells, EPO, SCF, IL-3, and IL-6 growth factors.
The cells are harvested from culture, and the CB-CD36+ cells
are positively isolated using a direct immunomagnetic CD36
MicroBead labeling system.
Application
CB-CD36+ cells can be used to:
• Study the parvovirus B19 tropism for erythroid cells.
• Replace the traditional CD71 + cells as an immediate
and abundant source for erythroid cell studies and
transplantation studies.
• Research erythropoiesis.
• Act as a control in therapeutic studies for early
erythroblastic leukemia and polycythemia vera (PV).
• Study production and functions of hemoglobin.
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
BM-CD36+ Cells
FL-CD36+ Cells
MPB-CD36 + Cells
www.allcells.com
For In Vitro Research Use Only
Cord Blood
Product Description
87
Cord Blood—Neutrophils
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB026
40 million/vial
$833
Comprehensive RNA
RNA-CB014C
1.0 µg
$387
cDNA
cDNA-CB014
10 PCR rxns
$275
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-Neutrophils
Cord Blood
Product Description
Purity
RBC depleted leukocytes are collected from cord blood by
sedimentation of red blood cells using HetaSep. Then, the
granulocytes are isolated by density gradient separation and
further processed to deplete any remaining RBC. Finally, the
neutrophils are isolated using an indirect immunomagnetic
labeling system.
Note: Polymorphonuclear Neutrophils (PMN) are the most
abundant white blood cells in human peripheral blood. The
average lifespan of non-activated PMN is approximately 12 to
24 hours. Generally, our PMN activation response is greater
than 50% when stimulated with fMLP or LPS chemokines.
Fresh PMN are shipped via FedEx Priority Overnight for nextday morning delivery.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
BM-Neutrophils
NPB-Neutrophils
Application
CB
•
•
•
•
Neutrophils can be used to study the following:
Oxidative metabolism
Inflammation responses
Phagocytic cell responses
Infant and neonatal immune response to bacterial
infections
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
88
Cord Blood—CD14 + Monocytes
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB022
5 million/vial
$780
Frozen
CB022F
5 million/vial
$716
Comprehensive RNA
RNA-CB004C
5.0 µg
$490
cDNA
cDNA-CB004
10 PCR rxns
$788
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Purity
Product Description
CB CD14+ Monocytes are positively selected from
mononuclear cells, using immunomagnetic CD14 MicroBeads.
Lead Time
Application
CB-CD14+ Monocytes can be used to study the following:
• Antigen presentation and phagocytic property
• Induced macrophage differentiation
• Dendritic cell differentiation
• Osteoclast differentiation
• HIV infection mechanisms
• Fc receptor binding and antibody research
ALLCELLS
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-Monocytes
NPB-Monocytes
www.allcells.com
For In Vitro Research Use Only
Cord Blood
CB-CD14+ Monocytes
89
Cord Blood—CD19 + B Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB021
5 million/vial
$770
Frozen
CB019F
1 million/vial
$432
CB020F
2.5 million/vial
$706
CB021F
5 million/vial
$733
Comprehensive RNA
RNA-CB003C
3.0 µg
$498
cDNA
cDNA-CB003
10 PCR rxns
$727
Molecular Products
HLA-Typed Products are available upon request.
Cord Blood
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD19+ B Cells
Product Description
Purity
CB-CD19+ B cells are positively isolated from mononuclear
cells, using a direct immunomagnetic CD19 MicroBeads
labeling system
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
Application
CD19+ B Cells can be used to study:
• Naïve B cell expansion
• T Cell regulation of immuno-activity
• Gene rearrangement of immunoglobulins
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-B Cells
NPB-B Cells
www.allcells.com
For In Vitro Research Use Only
90
Cord Blood—T Cells—CD3 + Pan T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB020-1
15 million/vial
$875
Frozen
CB020-1F
15 million/vial
$752
Comprehensive RNA
RNA-CB002C
5.0 µg
$415
cDNA
cDNA-CB002
10 PCR rxns
$293
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Purity
CB-Pan T cells are negatively isolated from mononuclear cells,
using an indirect immunomagnetic Pan T labeling system.
Application
Lead Time
Pan T cells can be used to:
• Study immunosuppression of GVHD in transplantation
experiments.
• Research their ability to generate anti-viral cytotoxic T
cells without prior (prenatal) exposure to viral antigens.
• Act as a control for childhood lymphocytic leukemia
studies.
ALLCELLS
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-Pan T Cells
NPB-Pan T Cells
www.allcells.com
For In Vitro Research Use Only
Cord Blood
CB-CD3+ Pan T Cells
91
Cord Blood— T Cells—CD4 + Helper T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB020-2
15 million/vial
$875
Frozen
CB020-2F
15 million/vial
$686
Comprehensive RNA
RNA-CB006C
5.0 µg
$406
cDNA
cDNA-CB006
10 PCR rxns
$381
Molecular Products
HLA-Typed Products are available upon request.
Cord Blood
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD4+ Helper T Cells
Purity
Product Description
Cord Blood CD4+ Helper T cells are negatively isolated from
mononuclear cells using an indirect immunomagnetic CD4+ T
cell labeling system to deplete the non-CD4+ cells.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
CB-CD4 Helper T cells can be used to study:
• The role of the T helper cells in intrauterine infections
cytokine release, and effector T cell pathways
• Antigen presentation and antigen-associated activation of
T helper cells for efficient immune response in children
• Classification of T cell subtypes
+
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-CD4+ Helper T Cells
NPB-CD4 + Helper T Cells
Published Papers
Yang, Li, et al. (2008) IL-21 and TGF-Beta are required for differentiation of human TH17 cells. National Institute of Health Public
Access, doi: 10.38/nature07021.
www.allcells.com
For In Vitro Research Use Only
92
Cord Blood—T Cells—CD8 + Cytotoxic T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB020-3
5 million/vial
$849
Frozen
CB020-3F
5 million/vial
$732
Comprehensive RNA
RNA-CB008C
5.0 µg
$411
cDNA
cDNA-CB008
10 PCR rxns
$775
Molecular Products
HLA-Typed Products are available upon request.
CB-CD8+ Cytotoxic T Cells
Purity
Product Description
CB-CD8+ Cytotoxic T cells are negatively isolated from mononuclear cells, using an indirect immunomagnetic CD8 labeling
system to deplete the non-CD8 + cells.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
CB-CD8+ Cytotoxic T cells can be used to study:
• Childhood immunological response to viral infection
• Memory cytotoxic T cell pathways and formation
• Potential cellular engineering to fight cancer cells and
tumors
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-CD8+ T Cells
NPB-CD8 + T Cells
www.allcells.com
For In Vitro Research Use Only
Cord Blood
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
93
Cord Blood—T Cells—CD4 +/CD45RA+ Naïve T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB020-4-1
5 million/vial
$721
CB020-4-2
10 million/vial
$1034
CB020-4-3
15 million/vial
$1262
CB020-4-1F
5 million/vial
$607
CB020-4-2F
10 million/vial
$897
CB020-4-3F
15 million/vial
$1183
Comprehensive RNA
RNA-CB007C
3.0 µg
$429
cDNA
cDNA-CB007
10 PCR rxns
$698
Frozen
Cord Blood
Molecular Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD4+/CD45RA+ Naïve T Cells
Purity
Product Description
First, CB-CD4+ T Cells are negatively isolated using an
indirect immunomagnetic CD4+ labeling system from
mononuclear cells. Next, CD45RO MicroBeads are used to
deplete the CD45RO+ population, leaving a purified CD4+/
CD45RA + Naïve T Cell population.
Application
CB-CD4+/CD45RA+ Naïve T Cells can be used to study:
• Ex vivo expansion capability of Naive T Cells
• Naive T cell differentiation to memory effector cells
• Comparison of adult CD4+/CD45RA+ Naive T Cells
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
NPB-CD4+/CD45RA+ T Cells
www.allcells.com
For In Vitro Research Use Only
94
Cord Blood—CD56 + Natural Killer Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB023
1 million/vial
$770
Frozen
CB023F
1 million/vial
$695
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD56+ Natural Killer Cells
Purity
Cord Blood CD56+ Natural Killer (NK) Cells are positively
isolated from MNC using a direct immunomagnetic CD56
MicroBeads labeling system.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
CB-CD56+ NK cells can be used to study the following:
• Neonatal infection control
• Anti-leukemia and anti-tumor effect by transplantation
(due to NK cells’ enhanced sensitivity to stimulation and
reduced potential for GVHD)
• Fc receptor binding and ADCC assay for potential use in
therapeutic antibody development
ALLCELLS
Toll Free Phone: 1-888-535-3444
Related Products
MPB-NK Cells
NPB-NK Cells
www.allcells.com
For In Vitro Research Use Only
Cord Blood
Product Description
95
Cord Blood—Human Umbilical Vascular Endothelial Cells (HUVEC)
Product Format
Catalog #
QTY/Unit
Price/Unit
Proliferating/Single
HUVEC-001
100 mm area, 80% Confluent
$874
Frozen/Single
HUVEC-001F
0.5 million/vial
$255
Frozen/Mixed
HUVEC-001F-M
0.5 million/vial
$183
HLA-Typed Products are available upon request.
Cord Blood
Media
Product Format
Catalog #
QTY/Unit
Price/Unit
HUVEC Basal Medium
HUVEC-002
500 ml
$67
HUVEC Stimulatory Supplement
HUVEC-003
10 ml
$68
HUVEC Stimulatory Supplement
HUVEC-004
50 ml
$210
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-HUVEC
Application
Product Description
Human umbilical cord veins are obtained from healthy donors.
Each cord vein is individually processed (or pooled for mixed
products) to isolate endothelial cells through collagenase
digestion and culture. Frozen HUVEC products are cryopreserved
at the end of the primary culture. HUVEC are guaranteed to
reach 15 population doublings when cultured with AllCells's
recommended medium and supplement.
HUVEC Basal Medium - When supplemented with FBS,
epidermal growth factors and hydrocortisone, HUVEC Basal
Medium is designed to support clonal growth of HUVEC. AllCells's
Basal medium should be used in combination with HUVEC Basal
Medium Supplement. This product contains L-glutamine, but does
not contain growth factors. pH 7.1 to 7.5
HUVEC Stimulatory Supplement -
10mL bottle
(10x): add to 90 mL of HUVEC Basal Medium
50 mL bottle (10x): add to 450 mL of HUVEC Basal Medium
This product contains Fetal Bovine Serum, Epidermal Growth Factor,
Basic Fibroblast Growth Factor, Vascular Endothelial Growth Factor,
Heparin, and Hydrocortisone.
Lead Time
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
HUVEC can be used to study:
• The model of vessel development and reconstruction
• Development of anti-angiogenesis drugs for
tumor therapeutics
• Vasodilation and vasoconstriction in hypertension
related research
• Atherosclerosis
• Leukocyte transport and migration mechanisms
Viability
The viability is at least 70% upon thaw.
Properties
HUVEC express the following differentiation cell markers: CD31
endothelial cell marker (>90%), CD54 (ICAM; >90%), CD62e (Eselectin; >90%), and von Willebrand factor (>90%), and uptake of
dil-Ac-LDL (>90%).
Published Papers
Li-Jia Zhang, et al. (2012) PI3K p110a Isoform-Dependent Rho GTPase Rac1 Activation Mediates H2S-Promoted Endothelial Cell
Migration via Actin Cytoskeleton Reorganization. PLoS ONE 7(9)
http://www.allcells.com/publications/0171allcells.pdf
Adams, Gregory N, et al. (2011) Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial
thrombosis. Blood, 117 (14).
http://www.allcells.com/publications/0165allcells.pdf
www.allcells.com
For In Vitro Research Use Only
96
Fetal Liver
Fetal Liver Table of Contents
Unprocessed Fetal Liver
97
Stem/Progenitor Cells - CD133+ 100
Mononuclear Cells98
Early Erythroid Progenitor Cells - CD36+
101
Stem/Progenitor Cells - CD34+ 99
Mesenchymal Stem Cells
102
Fetal Liver Cells & Tissue
AllCells has developed methodologies to isolate and purify high quality human fetal liver cells for your research needs.
The following human fetal liver cell products are available from AllCells:
•
Unprocessed Fetal Liver (Molecular Products)
•
Mononuclear Cells (FL MNC)
•
CD34+ Stem/Progenitor Cells (FL-CD34)
•
CD133 + Stem/Progenitor Cells (FL-CD133)
•
CD36 + Early Erythroid Progenitor Cells (FL-CD36)
•
Mesenchymal Stem Cells (FL MSC)
•
Lysate and Cell Pellet products available upon request
Background
Fetal liver is believed to be the first organ in human development that generates enucleated red blood cells. Fetal liver
macrophages are believed to be responsible for recycling expelled RBC nuclei. Fetal liver RNA and cDNA can provide
insight into such mechanisms.
Fetal Liver is obtained from fetal tissue at a gestation of 10-22 weeks. All consents and protocols have been approved by
an independent biomedical review board.
For more information visit: www.allcells.com/fetal-liver/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
97
Fetal Liver—Unprocessed Fetal Liver
Product Format
Catalog #
QTY/Unit
Price/Unit
Comprehensive RNA
RNA-FL010C
50.0 µg
$410
cDNA
cDNA-FL004
10 PCR rxns
$294
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Unprocessed Fetal Liver
Background
Lead Time
Fetal liver is believed to be the first organ in human development
that generates enucleated red blood cells. Fetal liver macrophages
are believed to be responsible for recycling expelled RBC
nuclei. Fetal liver RNA and cDNA can provide insight into such
mechanisms.
1-2 business days for frozen products. Product delivery is subject to
stock availability.
Fetal Liver
Application
Fetal liver RNA and cDNA may provide insight into the following:
• The transition of fetal liver to a fully functional digestive organ
post-partum
• Hematopoietic stem cell pool maintenance
• Early fetal hematopoiesis
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
98
Fetal Liver—Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen - 10-12 weeks gestation
FL004-T1-F
0.2 million/vial
$172
FL005-T1-F
1 million/vial
$388
FL006-T1-F
5 million/vial
$1037
FL007-T1-F
10 million/vial
$1837
FL008F-T2-F
0.2 million/vial
$118
FL009F-T2-F
1 million/vial
$323
FL010F-T2-F
5 million/vial
$972
FL011F-T2-F
10 million/vial
$1837
Comprehensive RNA
RNA-FL007C
50.0 µg
$390
cDNA
cDNA-FL003
10 PCR rxns
$280
Frozen - 13-24 weeks gestation
Molecular Products
Fetal Liver
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Fetal Liver—Mononuclear Cells
Product Description
Lead Time
Fetal liver is dissociated in an asceptic manner, followed by density
separation to enrich the mononuclear cells. The enriched cells are
frozen using our optimized cryopreservation medium. Fetal liver
MNC can also be used for RNA extraction and cDNA production.
1-2 business days for frozen products. Product delivery is subject
to stock availability.
Application
Fetal Liver MNC can be used to:
• Study erythroid and other hematopoietic precursor cells at the
fetal level.
• Act as control in fetal-maternal diagnostic studies.
• Identify markers responsible for liver cell adaptive capability in
the prenatal stage.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Related Products
BM-MNC
CB-MNC
MPB-MNC
NPB-MNC
www.allcells.com
For In Vitro Research Use Only
99
Fetal Liver—CD34 + Stem/Progenitor Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
FL-CD34-001F
0.1 million/vial
$204
FL-CD34-002F
0.5 million/vial
$567
FL-CD34-007F
5 million/vial
$4614
FL-CD34-012F
10 million/vial
$6141
RNA-FL001C
0.1 µg
$294
RNA-FL002C
0.5 µg
$585
RNA-FL003C
1.0 µg
$903
cDNA-FL001
10 PCR rxns
$702
Molecular Products
Comprehensive RNA
cDNA
HLA-Typed Products are available upon request.
Fetal Liver
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Fetal Liver—CD34+ Stem/Progenitor Cells
Purity
Product Description
First, fetal liver is homogenized. Then, a density separation gradient
media (1.077 g/ml density) is used to isolate the MNC. Next, a
direct immunomagnetic CD34 MicroBead labeling system is used
to positively isolate FL-CD34+ cells.
Fetal liver CD34+ cells can be used to:
• Study engraftment potential in comparison to bone marrow
and cord blood CD34+ cells.
• Research whether fetal liver hematopoietic progenitor cells
are more naïve or less lineage-restricted than CD34+ stem/
progenitor cell from other sites of adult blood tissues.
ALLCELLS
Lead Time
1-2 business days for frozen products. Product delivery is subject to
stock availability.
Application
The purity is guaranteed to be at least 90% as assessed by flow
cytometric analysis.
Toll Free Phone: 1-888-535-3444
Related Products
BM-CD34+ Cells
MPB-CD34+ Cells
CB-CD34+ Cells
NPB-CD34 + Cells
Published Papers
Park, Tea Soon, et al. (2012) Growth Factor-Activated Stem Cell
Circuits and Stromal Signals Cooperatively Accelerate Non-Integrated
iPSC Reprogramming of Human Myeloid Progenitorss. PLoS ONE
7(8). http://www.allcells.com/publications/0128allcells.pdf
www.allcells.com
For In Vitro Research Use Only
100
Fetal Liver—Stem/Progenitor Cells—CD133 + Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
FL-CD133-008F
0.1 million/vial
$287
FL-CD133-001F
0.5 million/vial
$880
FL-CD133-006F
5 million/vial
$6095
RNA-FL004C
0.1 µg
$302
RNA-FL005C
0.5 µg
$838
RNA-FL006C
1.0 µg
$1173
cDNA-FL002
10 PCR rxns
$1129
Molecular Products
Comprehensive RNA
cDNA
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Fetal Liver
Fetal Liver—CD133+ Stem/Progenitor Cells
Product Description
Fetal liver is homogenized, and then a density separation gradient
media (1.077 g/ml density) is used to isolate the mononuclear
cells. Next, a direct immunomagnetic CD133 MicroBead labeling
system is used to positively select FL-CD133+ cells. The majority of
CD133+ cells express the CD34 cell marker.
Purity
The purity is guaranteed to be at least 90% as assessed by flow
cytometric analysis.
Lead Time
Application
Fetal liver CD133+ cells can be used to:
• Study engraftment potential (in comparison to bone marrow
and cord blood CD133+ cells).
• Research whether fetal liver hematopoietic progenitor cells are
more naïve or less lineage-restricted than other CD133+ stem/
progenitor cell from other sites of adult blood tissues
ALLCELLS
Toll Free Phone: 1-888-535-3444
1-2 business days for frozen products. Product delivery is subject to
stock availability.
Related Products
BM-CD133+ Cells
www.allcells.com
CB-CD133 + Cells
MPB-CD133 + Cells
For In Vitro Research Use Only
101
Fetal Liver—CD36 + Early Erythroid Progenitor Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
FL-CD36-001F
1 million/vial
$574
RNA-FL008C
1.0 µg
$557
Molecular Products
Comprehensive RNA
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Fetal Liver—CD36+ Early Erythroid Progenitor Cells
Fetal Liver
Product Description
Purity
Fetal liver CD36+ Early Erythroid Progenitor cells are derived from
FL-CD34+ cells in culture. Fetal liver-CD34+ cells are isolated from
fetal liver MNC with a purity >90%. FL-CD34+ cells are cultured for
seven to eight days using StemSpan SFEM (StemCell Technologies,
Inc.), a serum free expansion media for hematopoietic cells, with the
addition of EPO, SCF, IL-3, and IL-6 growth factors. The cells are
harvested from culture, and the CD36+ cells are positively isolated,
using immunomagnetic CD36 MicroBeads.
Application
FL-CD36+ cells can be used to:
• Study the parvovirus B19 tropism for erythroid cells.
• Replace the traditional CD71 + cells as an immediate
and abundant source for erythroid cell studies and
transplantation studies.
• Research erythropoiesis.
• Act as a control in therapeutic studies for early
erythroblastic leukemia and polycythemia vera (PV).
• Identify markers responsible for liver cell adaptive capability in
the prenatal stage.
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 90% as assessed by flow
cytometric analysis.
Lead Time
1-2 business days for frozen products. Product delivery is subject to
stock availability.
Related Products
BM-CD36+ Cells
CB-CD36+ Cells
MPB-CD36 + Cells
www.allcells.com
For In Vitro Research Use Only
102
Fetal Liver—Mesenchymal Stem Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
FL-001F
0.75 million/vial
$690
HLA-Typed Products are available upon request.
Media
Product Name
Catalog #
QTY/Unit
Price/Unit
Fetal Liver Basal Medium
FL-002
450 ml
$60
Fetal MSC Stimulatory Supplement
FL-003
50 ml
$186
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Fetal Liver—Mesenchymal Stem Cells
Mesenchymal Stem Cells are derived from fetal liver MNC. The fetal
liver is homogenized, and the MNC are isolated using lymphocyte
separation medium (1.077 g/ml density) and cultured. These cells
have fibroblast and epithelial-like morphology.
Application
Fetal Liver Basal Medium is a growth medium for mesenchymal
stem cells and should be used in conjunction with Fetal MSC
Stimulatory Supplement.
Fetal MSC Stimulatory Supplement
Fetal MSC Stimulatory Supplement promotes proliferation of fetal
liver MSC.
Fetal Liver MSC can be used for the following:
• Cell differentiation studies
• Drug screening
• High throughput screening and toxicology
• Functional genomics research
• Feeder cells for embryonic and hematopoietic stem cells
Related Products
BM-MSC
Properties
These FL-MSC have fibroblast-like morphology and express
CD105 (>90%) and CD90 (>90%). MSC lack expression of
CD34, CD45, and CD11b.
Lead Time
1-2 business days for frozen products. Product delivery is subject to
stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Fetal Liver
Fetal Liver Basal Medium
Product Description
103
NON-HEMATOPOIETIC STEM CELLS & tissue
Non-Hematopoietic Stem Cells Table of Contents
Adipose-Derived Stem Cells104
Amniotic & Placental Stem Cells
Human Dental Tissue & Mesenchymal Stem Cells
106
105
Adipose-Derived Stem Cells - new for 2013
Over the past ten years, it has been recognized that fat is not only an energy reservoir, but also a rich source of multipotent stem cells.
Subcutaneous adipose depots are ubiquitous and easily accessible in large quantities with a minimal invasive procedure (by liposuction
aspiration). It has been described that stem and progenitor cells in the uncultured stromal-vascular fraction (SVF) from adipose tissue
usually amount to up to 3% of the whole cells, and this is 2,500-fold more than the frequency of stem cells in bone marrow. Adiposederived stem cells (ASC) are multipotent cells that can easily be extracted from adipose tissue, are capable of expansion in vitro, and have
the capacity to differentiate into multiple cell lineages, which have the potential for use in regenerative medicine. Adipose-derived stem
cells possess the ability to readily be expanded in vitro and the capacity to undergo adipogenic, osteogenic, chondrogenic,and myogenic
(cardiomyocyte and skeletal myocyte) differentiation.
Amniotic & Placental Stem Cells
Human placenta, besides playing a critical and fundamental role in fetal development, nutrition and tolerance, represents a significant
source of stem and progenitor cells. Four regions of the fetal placenta can be distinguished: amniotic epithelia, amniotic mesenchye,
chorionic mesenchyme and chorionic trophoblast. From these regions, the following cell populations are isolated: human amniotic epithelial
cells (hAEC) and human chorionic mesenchymal stem cells (hCMSC). The minimal criteria for defining hAEC and hCMSC are as follows:
adherence to plastic, formation of fibroblast colony-forming units and differentiation into osteogenic, adipogenic, chondrogenic, vascular/
endothelial and neurogenic lineages. Both cell types are positive for CD90, CD73 and CD105 and negative for CD34, CD45, and CD11b.
Human Dental Tissue & Mesenchymal Stem Cells
Stem cells have been isolated from many tissues and organs, including dental tissue. Five types of dental stem cells have been
established: dental pulp stem cells (DPSC), stem cells from human exfoliated deciduous teeth (SHED), stem cells from apical papilla
(SCAP), periodontal ligament stem cells (PDLSC), and dental follicle stem cells (DFSC). The main characteristics of dental stem cells
are their potential for multilineage differentiation and self-renewal capacity. Dental stem cells can differentiate into odontoblasts,
adipocytes, neuronal-like cells, glial cells, osteoblasts, chondrocytes, melanocytes, myotubes, endothelial cells and pancreatic beta cells.
The multipotency of dental stem cells may be attributable to their embryonic origin which is the neural crest. The neural crest contains
pluripotent cells that can give rise to neurons and glial cells of the peripheral nervous system, endocrine cells, connective tissue cells,
muscle cells and pigment cells during embryonic development. Studies have demonstrated that subpopulations of stem cells residing in
these tissues expressed embryonic stem cell markers (Oct-4, Sox-2, Nanog and Klf-4) and a subset of neural crest markers (Nestin, Slug,
p75 and Sox10). Similar to bone marrow mesenchymal cells, DPSCs, SHED, DFSC and PDL express CD13, CD44, CD90, CD146 and
CD166, CD 105 and Stro-1, but are negative for CD3, CD8, CD11b, CD14, CD15, CD19, CD33, CD34, CD45, and HLA-DR. The lack
ofe xpression of class-I HLA antigens suggests that Dental Stem Cells have immunomodulatory capabilities similar to bone marrow MSCs.
Adipose-derived Stem Cells Background References:
•
Baer PC, Geiger H. Adipose-derived mesenchymal stromal/stem cells: tissue localization, characterization, and heterogeneity. Stem Cells Int. 2012;2012:812693.
•
Gimble JM, Katz AJ, Bunnell BA Adipose-derived stem cells for regenerative medicine. Circ Res. 2007 May 11;100(9):1249-60.
For more information visit: www.allcells.com/non-hematopoietic-stem-cells/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
104
Non-Hematopoietic Stem Cells—Adipose-derived Stem Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ASC001F
1 million/vial
$250
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Adipose-derived Stem Cells
Product Description
AllCells’ adipose-derived stem cells (ASC) are isolated from single donor, P3 from the stromal vascular fraction of lipoaspirates
(obtained by conventional vacuum-assisted or ultrasound –assisted liposuction methods) through culturing on plastic. ASCs are
cultured up to third passage, harvested, and characterized. They can be identified by means of fluorescence-activated cell sorting,
as they have several specific surface markers: CD73+, CD90+, CD105+, CD45–, CD34–, CD14 or CD11b, CD79– or CD19–, and
HLA-DR6. Fresh cells, stromal vascular fraction, and lipoaspirates are available upon request.
Application
Can be used for experimental (non-clinical) research on wound healing, soft tissue defects, inflammatory bowel disease
(Crohn’s disease), autoimmune diseases, critical limb ischaemia, cardiovascular diseases
Non-Hematopoietic: Adipose-derived
•
Lead Time
Same as for other frozen cell products (1-2 business days)
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
105
Non-Hematopoietic Stem Cells—Amniotic and Placental Stem Cells
Product Format
Cell Type
Catalog #
QTY/Unit
Price/Unit
Frozen
Amnionitc Fluid Stem Cells
(hAFSC)
HAF001F
1 million/vial
$976
Frozen
Amniotic Epithelial Stem
Cells (hAEC)
HAF002F
1 million/vial
$965
Frozen
Chorionic Mesenchymal
Stem Cells (hCMSC)
HC001F
1 million/vial
$976
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Amniotic and Placental Stem Cells
Non-Hematopoietic: Amniotic
Product Description
Fluid Phase Amniotic Stem Cells (FPASC) are obtained from human amniocentesis specimens. FPASC represent about 1% of
the cells found in amniotic fluid. They are characterized by slow proliferation in culture for approximately 1 week, become adherent
to plastic and can be passaged at 70% confluency every two to three days, retaining a normal karyotype. Purification of isolated
FPASc can be readily performed by selection for expression of the membrane stem cell factor receptor c-Kit. In addition, FPASC
express embryonic markers such as Oct-4 and SSEA-4. They also express mesenchymal and/or neuronal markers such as CD29,
CD44, CD73, CD90 and CD105. FPASCs can be directed into a wide range of cell types representing the three primary
embryonic lineages of mesoderm, endoderm and ectoderm, including cells of adipogenic, osteogenic, myogenic, endothelial,
neuronal and hepatic lineages. They have a very high renewal capacity and can be expanded for over 250 population doublings
without any loss of chromosomal telomere length.
Human Amniotic Epithelial (HAE) Cells are isolated from the surface layer of the amniotic membrane of fresh placentas. HAE
cells retain embryonic stem cell like characteristics and express CD117 (c-Kit) and SSEA-3 and -4. HAEC also express molecular
markers of pluripotent stem cells including Nanog, Oct-4 and Sox-2. These cells are useful for tissue differentiation of all three
germ layers, including the ectoderm, endoderm, and the mesoderm. Some examples of tissue differentiation applications include
conversion into pancreatic islet cells, hepatocyte like cells, cardiomyocytes and neuron like cells.
Human Chorionic Mesenchymal Stem Cells (hCMSC) are derived from extraembryonic mesoderm and are isolated from fresh
chorion following mechanical and enzymatic removal of the trophoblastic layer. They adhere and proliferate well in cell culture and
retain their stem cell characteristics beyond passage 10. Differentiation of hCMSC to all three germ layers, ectoderm (neural),
mesoderm (skeletal muscle, cardiomyocytic and endothelial) and endoderm (pancreatic) has been reported. The embryonic stem
cell marker SSEA-4 has been identified on hCMSC as well as CD349 and CD140b.
Application
•
•
Considered an attractive candidate progenitor cell type for tissue engineering applications.
Can be frozen and thawed and are adherent to plastic.
Lead Time
Two weeks lead time is needed for FPASC, 1-2 business days for HAE or hCMSC
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
106
Non-Hematopoietic Stem Cells—Dental Pulp Tissue and Stem Cells
Product Format
Cell Type
Catalog #
QTY/Unit
Price/Unit
Frozen
Processed Tissue,
Deciduous Teeth
DP001F
1 million/vial
$655
Frozen
Processed Tissue,
Adult 3rd Molar
DP002F
1 million/vial
$655
Follicular Tissue
FT001F
1 million/vial
$655
Follicular Stem Cells
FT002F
1 million/vial
$759
Frozen
Dental Pulp Stem Cells
Adult Teeth
DP003F
1 million/vial
$759
Frozen
Dental Pulp Stem Cells
Deciduous Teeth
DP004F
1 million/vial
$759
Frozen
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Dental Pulp Tissue—Mesenchymal Stem Cells
Stem Cells From Human Exfoliated Deciduous Teeth (SHED) are considered to be the most ‘immature’ of the Dental Stem
Cells and show the highest proliferative capacity when grown in culture. Similar to the DPSCs from third molars, SHED are very
durable in culture and have been passaged over 80 times with no detectable signs of senescence or telomere shortening. SHED
express the embryonic stem cell (ESC) markers Oct-4, Nanog, SSEA-3 and -4, and tumor recognition antigens (TRA-1-60 and
TRA-1-81). When cultured in neurogenic medium, SHED form sphere-like clusters which adhere to the culture dish or float freely
in the culture medium.
Dental Follicle Stem Cells is an ectomesenchymal tissue surrounding the enamel organ and the dental papilla of the developing
tooth germ prior to eruption. The tissue contains the progenitor cells that form the periodontium, (cementum, PDL, and alveolar
bone). In culture DFSC adhere to plastic, proliferate well and like other mesenchymal stem cells show a typical fibroblast-like
morphology. DFSCs express the embryonic marker Oct-4 and the neural progenitor markers Notch-1 and nestin.
Dental Pulp Stem Cells were primarily derived from the pulp tissues of exfoliated deciduous teeth, primary incisors and permanent
third molar teeth. The dental pulp, third molars in particular, have been shown to be a significant stem cell source due to greater
numbers of stem cells residing in the pulp. DPSCs from third molars have been shown to express developmentally important
transcription factors, such as Oct-4, Sox-2, Nanog and c-Myc, which is an indicator of their pluripotency. Flow-cytometric analysis
revealed DPSCs were positive for CD73, CD90, CD105 and CD166, but negative for CD34, CD45 and CD133, suggesting that
these cells are mesenchymal-like stem cells. Under specific culture conditions, DPSCs differentiated into osteogenic, adipogenic and
neurogenic cells, as well as formed tube-like structures in Matrigel assay.
Application
•
•
Good candidates for future research as a new, powerful tool for therapy.
The efforts made in the research of dental stem cells have clarified many mechanisms underlying the biological processes in
which these cells are involved.
Lead Time
1-2 business days for frozen products. Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Non-Hematopoietic: Dental
Product Description
107
Diseased CellS & tissue
This image was originally published in ASH Image Bank.
Peter Maslak. Primary induction failure: AML - 1. ASH Image Bank. 2011; 2011-1298. The American
Society of Hematology.
This image was originally published in ASH Image Bank.
Gridhar U Adiga; Peter C Cote'; Cheryl L Gardner. Bone marrow necrosis in myelodysplastic syndrome - 5. ASH Image Bank. 2011; 2011-1250. The American Society of Hematology.
ALLCELLS
Toll Free Phone: 1-888-535-3444
This image was originally published in ASH Image Bank.
Ruth Rose. Granular ALL - 1. ASH Image Bank. 2011; 2011-2723. The American Society of
Hematology.
This image was originally published in ASH Image Bank.
John Lazarchick. Leukemic phase of multiple myeloma - 1. ASH Image Bank. 2011; 2011-4094.
The American Society of Hematology.
www.allcells.com
For In Vitro Research Use Only
108
Diseased CellS & tissue
Diseased Cells & Tissue Table of Contents
Custom Products109-110
New Products111
Leukemia112-136
Multiple Myeloma (MM)
137-142
Autoimmune143-150
Sickle Cell Anemia (SCA)
151
Inflammatory Bowel Disease (IBD)
152-154
Diseased Cells & Tissue
Resulting from numerous customer requests, AllCells has established a nationwide network of clinical collection sites to supply various types of disease cells for research use. These facilities supply clinically-relevant, peripheral blood and bone marrow
with sample medical history for use in pre-clinical studies. We have amassed a cell repository of patient samples for your
research needs. This repository is updated on a daily basis.
For custom collections and projects visit us on the web at www.allcells.com/technical-support/ or
contact us at [email protected].
Patient's Clinical Information
A Clinical Report may be available for each diseased product. The report includes patient information, age, gender, race,
weight, height; classification of the disease and staging; and treatment history.
The Clinical Report is a clinical history of the patient and not all tests are performed at the time of the blood sample draw.
Please note that all test results are not always concurrent or reflective of each other.
Certificate of Analysis
A Certificate of Analysis is provided with the product information; this document details the cell number and the cell viability
of the product vial.
Please note that due to the nature of the disease and sensitivity of the diseased patients, product availability is limited.
For more information visit: www.allcells.com/diseased/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
109
CUSTOM PRODUCTS
Non-Hodgkin's Lymphoma Cells & Tissue
AllCells is able to provide custom collections of B-cell lymphomas, including Diffuse Large B-cell Lymphoma (DLBCL),
Mantle Cell Lymphoma (MCL), Follicular Lymphoma (FLL), and Waldenstrom's Macroglobulinemia (WM) from either bone marrow and/or peripheral blood samples of newly diagnosed/untreated or relapsed/refractory patients. Medical history of each
patient is available and may include treatment history, serum biochemistry, bone marrow aspirate smear analysis, cytogenics,
and immunophenotyping.
Background
Lymphomas are types of cancers derived from lymphocytes and are typically treated by combinations of chemotherapy,
monoclonal antibodies, immunotherapy, radiation, and hematopoietic stem cell transplantation.
Non-Hodgkin’s Lymphoma (NHL) is a collective term for a broad range of lymphomas that are not classified as Hodgkin’s
lymphoma. NHL can either be a cancer of B cells or T cells. B-cell non-Hodgkin lymphomas include Burkitt’s lymphoma,
diffuse large B-cell lymphoma, follicular lymphoma, immunoblastic large cell lymphoma, precursor B-lymphoblastic
lymphoma, and mantle cell lymphoma. T-cell non-Hodgkin lymphomas include anaplastic large cell lymphoma, and precursor
T-lymphoblastic lymphoma.
For custom collections and projects visit us on the web at www.allcells.com/technical-support/ or
contact us at [email protected].
Please note that due to the nature of the disease and sensitivity of the diseased patients, product availability is limited.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
110
Custom Products—Non-Hodgkin's Lymphoma
DLBCL
Follicular Lymphoma (FLL)
Follicular Lymphoma (FLL) is a type of “indolent” NonHodgkin’s Lymphoma and is defined by follicule center B
Cells, which express a follicular pattern. FLL’s genetic origin
has been correlated to t(14;18), which causes excessive
expression of the Bcl-2 gene, and some reported cases Bcl6 translocation at 3q27. Typically, follicular B cells express
surface-markers CD10, but not CD5. The “tumors” or lymph
follicles contain a mixture of “small” cleaved follicle center
cells (centrocytes) and “large” non-cleaved follicle center
cells (centroblasts): the former usually in predominance. FLL
is generally indolent but can sometimes progress to more
aggressive large cell lymphomas, such as DLBCL.
Diffuse Large B Cell Lymphoma (DLBCL)
Diffuse Large B Cell Lymphoma (DLBCL) is an “intermediategrade” and aggressive type of malignant B cell lymphoma.
DLBCL can arise from an immunoblastic origin, or can
transform from the follicule center large cell neoplasm.
This transformed cell can be further classified into large
noncleaved (centroblastic) cells or large cleaved (large
centrocytic) cells. DLBCL is frequently associated with
t(8;14) and t(14;18): chromosomal rearrangements pertaining
to Myc and Bcl-2 genes. In addition, there may be some
chromosomal mutation involving Bcl-6 on chromosome 3.
Waldenström's Macroglobulinemia (WM)
Waldenström’s Macroglobulinemia (WM), also known as
lymphoplasmacytic lymphoma, WM is one of the rarest types
of NHL. WM is a malignant small lymphocytic lymphoma
with plasmacytoid features. While this disease has not been
linked to a single mutation, researchers have found a WM
associated with the 6p21.3 locus. WM is characterized by
large monoclonal IgM in the serum and symptoms, such as
peripheral neuropathy and hyperviscosity syndrome. WM is
distinguished from Multiple Myeloma by its indolent nature and
less frequent bone marrow involvement. Patient monitoring is
usually recommended before any necessary treatment.
DLBCL can also be called “histiocytic” lymphoma, which
refers to the morphologic expression of the transformed
lymphocytes. DLBCL is the most frequent type of extranodal
lymphoma with the least common bone marrow involvement.
Mantle Cell Lymphoma (MCL)
Mantle Cell Lymphoma (MCL) is one of the rarer types of NHL.
MCL is a disease of the naïve pre-germinal center B cells,
located in the “mantle zone” surrounding the normal germinal
center follicles. A distinctive translocation at t(11;14)(q13;q32)
causes upregulation of Bcl-1, development arrest, and unlimited
proliferation of the mantle-zone B cells. This chromosomal
translocation causes the over-expression of Bcl-1’s protein
product, Cyclin-D1. In addition, these mantle-zone B cells
express the CD5 marker. MCL is one of the few NHLs that can
cross the blood-brain barrier.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Diseased Cells: Custom Products
This image was originally published in ASH Image Bank.
John Lazarchick. Lymphomatous peritoneal effusion - 3. Mature B-cell neoplasms. ASH Image
Bank. 2011; 2011-3729. The American Society of Hematology.
111
New Products
Autoimmune Diseased Cells & Tissue
AllCells recently began carrying plasma, peripheral blood and/or isolated cell types from patients diagnosed with Autoimmune diseases such as Rheumatoid Arthritis, Osteoarthritis, Systemic Lupus Erythematosus (SLE), Psoriasis, Type I &
Type II Diabetes, and Multiple Sclerosis. Patient treatment history and clinical reports are available for each sample, and
may include auto antibody analysis and/or clinical diagnostic scores (ACR, SLEDAI, etc.). Autoimmune Diseased Cells
& Tissue products are obtained from volunteers participating in an Institutional Review Board (IRB) or Human Subject
Committee approved donor program. All donors sign an informed consent form, and are screened for infectious diseases
(HIV, Hepatitis B & C). Find our entire inventory of Autoimmune Cells & Tissue on pg. 143 of the catalog.
Inflammatory Bowel Diseased Cells & Tissue
AllCells sources samples of Inflammatory Bowel Disease (IBD) cells isolated from Crohn’s Disease and Ulcerative Colitis
patients. IBD plasma, peripheral blood, and peripheral blood mononuclear cells are available from each disease type.
These are available in frozen, fresh formats upon request. Patient treatment history and clinical reports are available for
each sample. View our entire inventory of IBD Cells & Tissue on pg. 152 of the catalog.
MDS & Sickle Cell Anemia Diseased Cells & Tissue
We now isolate mononuclear cells (MNCs) from the bone marrow of MDS (myelodysplastic syndrome) patients. Patients
participating in our MDS donor program have been characterized as either Newly Diagnosed/Untreated or Relapsed/
Refractory. Medical history of each patient is available which may include treatment history, serum biochemistry, bone
marrow aspirate smear analysis, cytogenics, and immunophenotyping.
AllCells has begun carrying peripheral blood from patients afflicted with Sickle Cell Anemia. Mononuclear cells (isolated
from peripheral blood), peripheral blood, and plasma are available. Medical history of each patient is available and may
include treatment history.
All diseased cells and tissue products are obtained from volunteers participating in an Institutional Review Board (IRB) or
Human Subject Committee approved donor program. Donors sign an informed consent form and are aware of the fact that
these cells are used for research purposes only. Donors must be over the age of 18.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
112
Leukemia cells & tissue
Leukemia Table of Contents
Leukemia
Background113
Acute Lymphoid Leukemia (ALL)
114-119
ALL Peripheral Blood Plasma
114
ALL Peripheral Blood Mononuclear Cells
115
ALL Peripheral Blood CD19+ B Cells
116
ALL Bone Marrow Plasma
117
ALL Bone Marrow Mononuclear Cells
118
ALL Bone Marrow CD19+ B Cells
119
Acute Myeloid Leukemia (AML)
120-124
AML Peripheral Blood Plasma
120
AML Peripheral Blood Mononuclear Cells 121
AML Bone Marrow Plasma
122
AML Bone Marrow Mononuclear Cells
123
AML Bone Marrow CD34+ Cells
124
Myelodysplastic Syndrome (MDS)
Chronic Lymphoid Leukemia (CLL)
CLL Peripheral Blood Plasma
CLL Peripheral Blood Mononuclear Cells
CLL Peripheral Blood CD19+/CD5+ B Cells
CLL Bone Marrow Plasma
CLL Bone Marrow Mononuclear Cells
CLL Bone Marrow CD19+/CD5+ B Cells
Chronic Myeloid Leukemia (CML)
CML Peripheral Blood Plasma
CML Peripheral Blood Mononuclear Cells
CML Bone Marrow Plasma
CML Bone Marrow Mononuclear Cells
CML Bone Marrow CD34+ Cells
125
126-131
126
127
128
129
130
131
132-136
132
133
134
135
136
Leukemia Cells & Tissue
AllCells specializes in highly purified mononuclear cells from peripheral blood and bone marrow. We also provide specifically selected
leukemia blood cells from patients. These samples are made available from newly diagnosed or relapsed/refractory patients and can be
provided in frozen, fresh formats depending on availability. Please contact us for any other special requests.
A Certificate of Analysis (CofA) for each product will be provided with the shipment. The information about the patient includes their age,
gender, race, weight, height, classification of the disease, the treatment history and the status of the disease when the cells were obtained.
The CofA also includes information about the product such as the number of cells and the viability of the cells in the product vial.
The clinical reports for each product will be provided with the shipment. Medical history of each patient is available and may include treatment history, serum biochemistry, bone marrow aspirate smear analysis, cytogenics, and immunophenotyping.
Background
Leukemia refers to a cancer of the blood-forming (hematopoietic) tissues found in the body. This cancer starts in the bone
marrow and usually spreads quickly into the blood. Over time, leukemia can spread to the lymph nodes, the spleen, the liver,
the covering of the brain and spinal cord, the spinal fluid, and other organs.
There are two ways to classify leukemia: acute or chronic and lymphoid or myeloid. In acute leukemia, the leukemia cells
are derived from early progenitor cells, the immature “blasts.” In chronic leukemia, the leukemia cells are derived from mature lineage-specific, abnormal cells. These cells live longer than normal cells and build up over time, causing tumors. Lymphoid leukemia develops from lymphoblasts or lymphocytes in the bone marrow. Myeloid leukemia develops from myeloid
cells in the bone marrow. Consequently, there are four major types of leukemia: acute lymphoid leukemia, acute myeloid
leukemia, chronic lymphoid leukemia, and chronic myeloid leukemia.
For more information visit: www.allcells.com/leukemia/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
113
Leukemia—Types—Background
Acute Lymphoid Leukemia (ALL)
Acute lymphoblastic leukemia (ALL), is a form of leukemia,
or cancer of the white blood cells, characterized by excess
lymphoblasts. Malignant, immature white blood cells continuously multiply and are overproduced in the bone marrow. ALL
causes damage and death by crowding out normal cells in
the bone marrow and by spreading (metastasizing) to other
organs.
Acute Myeloid Leukemia (AML)
Acute myeloid leukemia (AML), also known as acute myelogenous leukemia, is a cancer of the myeloid line of blood
cells, characterized by the rapid growth of abnormal white
blood cells that accumulate in the bone marrow and interfere
with the production of normal blood cells. AML is the most
common acute leukemia affecting adults, and its incidence
increases with age. As an acute leukemia, AML progresses
rapidly and is typically fatal within weeks or months if left
untreated.
Diseased Cells: Leukemia
Myelodysplastic Syndrome (MDS)
The myelodysplastic syndromes (MDS, formerly known as
“preleukemia”) are a diverse collection of hematological conditions united by ineffective production (dysplasia) of myeloid
blood cells; these conditions risk transformation to acute myelogenous leukemia (AML). Myelodysplastic syndromes are
bone marrow stem cell disorders resulting in disorderly and
ineffective hematopoiesis manifested by irreversible quantitative and qualitative defects in hematopoietic cells. In a majority
of cases, the course of disease is chronic with gradually
worsening cytopenias due to progressive bone marrow failure.
Approximately one-third of patients with MDS progress to
AML within months to a few years.
Chronic Lymphoid Leukemia (CLL)
Chronic Lymphoid Leukemia (CLL) also known as B cell
chronic lymphocytic leukemia (B-CLL) is a slow growing cancer of the blood that affects older adults. Chronic
Lymphoid Leukemia cells originate in the bone marrow and
then spread to the blood and lymph nodes. Characterization
by flow cytometry demonstrates that these CLL cells are
CD19+ and CD5+. Prognosis of CLL is dependent on the
cytogenic profile of the cancer. Deletions of part of the short
arm of chromosome 17 (del 17p) or deletions of the long arm
on chromosome 11 (del 11q) indicate a poorer outcome while
deletions of the long arm of chromosome 13 (del 13q) favors
a better prognosis for CLL patients.
Chronic Myeloid Leukemia (CML)
Chronic myelogenous (or myeloid) leukemia (CML), also
known as chronic granulocytic leukemia (CGL), is a form of
leukemia characterized by the increased and unregulated
growth of predominantly myeloid cells in the bone marrow
and the accumulation of these cells in the blood. CML is a
clonal bone marrow stem cell disorder, in which proliferation
of mature granulocytes (neutrophils, eosinophils, and basophils) and their precursors is the main finding. CML was the
first malignancy to be linked to a clear genetic abnormality
— the chromosomal translocation known as the Philadelphia
Chromosome.
CML
This image was originally published in ASH Image Bank.
Peter Maslak. Relapsed CML - 1. Bone Marrow aspirate from a patient with increasing WBC
count on imanitib. ASH Image Bank. 2011; 2011-1300. The American Society of Hematology.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
114
Acute Lymphoid Leukemia
Diseased Cells—ALL—Peripheral Blood—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Newly Diagnosed/Untreated)
ALL-PB011F
5 ml
$191
Frozen (Relapsed/Refractory)
ALL-PB012F
5 ml
$191
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
ALL-PB-Plasma
Product Description
ALL-PB-Plasma is obtained by centrifugation of ALL peripheral
blood. This product has a diminished number of cells. ALL-Plasma
are available in the newly diagnosed/untreated and relapsed/refractory stages.
Related Products
ALL-BM-MNC
NPB-Plasma
Application
Newly Diagnosed/Untreated - ALL-PB-Plasma can be used to
study ALL lymphoblast and lymphocyte secretion in the plasma.
Relapsed/Refractory - ALL-PB-Plasma can be used to study drug
resistant ALL-lymphoblast secretion and pathway proteins.
Diseased Cells: Leukemia: ALL
Lead Time
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
115
Diseased Cells—ALL—Peripheral Blood—Mononuclear Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ALL-PB001F
10 million/vial
$635
Comprehensive RNA
RNA-ALL-PB001C
5.0 µg
$572
cDNA
cDNA-ALL-PB001
10 PCR rxns
$572
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ALL-PB003F
10 million/vial
$635
Comprehensive RNA
RNA-ALL-PB002C
5.0 µg
$572
cDNA
cDNA-ALL-PB002
10 PCR rxns
$572
Relapsed/Refractory
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
ALL-PB-MNC
Diseased Cells: Leukemia: All
Product Description
PB-MNC are isolated from ALL peripheral blood by diluting the
whole blood with PBS and using gradient separation techniques.
After centrifugation, the mononuclear cell layer is collected. MNC
can be processed further to isolate subpopulations. ALL-MNC are
available in the newly diagnosed/untreated and relapsed/refractory
stages.
Application
ALLCELLS
Lead Time
Product delivery is subject to stock availability.
Newly Diagnosed/Untreated - ALL-PB-MNC can be used to:
• Research better methods of ALL detection and diagnosis.
• Help distinguish between T and B cell blasts.
• Test for the presence of Philadelphia Chromosome.
• Study various mutations under ALL.
• Research new drug candidates, including various molecular
and genetic drug delivery methods.
• Study any negative effects of treatment on non-malignant cells
and to help minimize damage to non-leukemia cells.
Relapsed/Refractory - ALL-PB-MNC can be used to study:
• In vitro drug research, drug delivery for antibody
and small molecules, and/or therapies that target resistance to
primary treatments
• Anti-metastasis drugs or therapies
• Minimizing drug toxicity to non-leukemia cells
Toll Free Phone: 1-888-535-3444
Related Products
ALL-BM-MNC
NPB-MNC
www.allcells.com
For In Vitro Research Use Only
116
Diseased Cells—ALL—Peripheral Blood—CD19 + B Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ALL-B006F
5 million/vial
$1505
ALL-B007F
10 million/vial
$2208
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
ALL-PB-CD19+ B Cells
Product Description
Purity
ALL-PB-CD19+ B cells are positively isolated from adult ALL
peripheral blood MNC, using a direct immunomagnetic CD19
MicroBead labeling system. ALL-PB-CD19+ B cells are available in
the newly diagnosed/untreated and relapsed/refractory stages.
The purity is guaranteed to be at least 90% as assessed by flow
cytometric analysis.
Lead Time
Application
Newly Diagnosed/Untreated - ALL-CD19+ B Cells can be used
for cytogenetic and mutational analysis of B-ALL diseases, such as
ALL-L3 or Burkitt’s lymphoma/leukemia.
Related Products
ALL-BM-CD19+ B Cells
NPB-BM-CD19 + B Cells
Diseased Cells: Leukemia: ALL
Relapsed/Refractory - ALL-CD19+ B Cells can be used for
therapeutic methods, targeting drug resistance in ALL-L3 or
Burkitt’s lymphoma/leukemia.
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
117
Diseased Cells—ALL—Bone Marrow—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Newly Diagnosed/Untreated)
ALL-BM011F
5 ml
$191
Frozen (Relapsed/Refractory)
ALL-BM012F
5 ml
$191
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
ALL-BM-Plasma
Lead Time
Product Description
ALL BM plasma is obtained by centrifugation of the ALL-bone
marrow. This product has a diminished number of cells and platelets.
ALL plasma are available in the newly diagnosed/untreated and
relapsed/refractory stages.
Product delivery is subject to stock availability.
Related Products
ALL-PB-Plasma
Application
Newly Diagnosed/Untreated - ALL-BM-Plasma can be used to
research B- lymphoblast secretion and activity in bone marrow.
Diseased Cells: Leukemia: All
Relapsed/Refractory - ALL-BM-Plasma can be used to study cell
secretion and proteins in the bone marrow plasma of non-responsive, post-treatment patients and cellular resistance to primary
treatments.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
118
Diseased Cells—ALL—Bone Marrow—Mononuclear Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ALL-BM001F
10 million/vial
$635
Comprehensive RNA
RNA-ALL-BM001C
5.0 µg
$572
cDNA
cDNA-ALL-BM001
10 PCR rxns
$572
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ALL-BM003F
10 million/vial
$635
Comprehensive RNA
RNA-ALL-BM002C
5.0 µg
$572
cDNA
cDNA-ALL-BM002
10 PCR rxns
$572
Relapsed/Refractory
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
ALL-BM-MNC
Product Description
Lead Time
Product delivery is subject to stock availability.
Diseased Cells: Leukemia: ALL
BM is obtained from adult ALL patients. BM-MNC are isolated from
bone marrow by diluting the whole bone marrow with phosphatebuffered saline and using gradient separation techniques. MNC can
be processed to isolate subpopulations. ALL-BM-MNC are available
in the newly diagnosed/untreated and relapsed/refractory stages.
Related Products
ALL-PB-MNC
BM-MNC
Application
Newly Diagnosed/Untreated - ALL-BM-MNC can be used to:
• Research diagnosis and detection methods.
• Purify B and T lymphoblasts.
• Act as a possible source for isolating leukemia stem cells.
Relapsed/Refractory - ALL-BM-MNC can be used to research:
• In vitro therapeutic methods targeted against drug-resistant
leukemia stem cells
• Leukemia stem cell pools
• Drug delivery methods
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
119
Diseased Cells—ALL—Bone Marrow—CD19 + B Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ALL-B012F
5 million/vial
$1271
ALL-B013F
10 million/vial
$2034
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ALL-B016F
10 million/vial
$2034
Relapsed/Refractory
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
ALL-BM-CD19+ B Cells
Application
Background
New ALL-BM research has discovered the co-expression of
CD19 surface markers on BM-blast cells, notably in the cases
where t(8;21) translocation is found. Non-myeloid markers in ALL
(especially of B-cell specific transcription factor PAX5 and its
product, BSAP) activates most of the B cell-related genes, such as
CD19 and CD79b.
Diseased Cells: Leukemia: All
Product Description
ALL-BM-CD19+ B cells are positively isolated from adult ALL-BMMNC, using a direct immunomagnetic CD19 MicroBead labeling
system. These CD19+ B cells are are available in newly diagnosed/
untreated and relapsed/refractory stages.
ALL-BM-CD19+ can be used to:
• Study leukemia stem/progenitor cells for precursor B acute
lymphoblastic leukemia/lymphoma.
• Research molecular mechanisms for B cell loss of function and
malignancy.
• Serve as diagnostic tools to aid classification of various subtypes of B-ALL.
• Develop therapeutic approaches.
Purity
The purity is guaranteed to be at least 90% as assessed by flow
cytometric analysis.
Lead Time
Product delivery is subject to stock availability.
Related Products
ALL-PB-CD19+ B Cells
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
120
Acute Myeloid Leukemia
Diseased Cells—AML—Peripheral Blood—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Newly Diagnosed/Untreated)
AML-PB011F
5 ml
$195
Frozen (Relapsed/Refractory)
AML-PB012F
5 ml
$195
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
AML-PB-Plasma
Product Description
AML-PB-Plasma is obtained by centrifugation of AML peripheral
blood. AML-PB-plasma has a diminished number of cells and
platelets. AML plasma is available in the newly diagnosed/untreated
and relapsed/refractory stages.
Related Products
AML-BM-Plasma
NPB-Plasma
Application
AML-PB-Plasma can be used to study and analyze leukemia cell
and progenitor cell protein secretion into the serum or plasma.
Lead Time
Diseased Cells: Leukemia: Aml
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
121
Diseased Cells—AML—Peripheral Blood—Mononuclear Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
AML-PB001F
10 million/vial
$634
Comprehensive RNA
RNA-AML-PB001C
5.0 µg
$572
cDNA
cDNA-AML-PB001
10 PCR rxns
$572
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
AML-PB003F
10 million/vial
$634
cDNA
cDNA-AML-PB002
10 PCR rxns
$572
Relapsed/Refractory
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
AML-PB-MNC
Diseased Cells: Leukemia: Aml
Product Description
Lead Time
PB-MNC are isolated from AML-PB by diluting the
blood with phosphate-buffered saline and using gradient
separation techniques. After centrifugation, the MNC layer
is collected. MNC can be processed further to isolate
subpopulations. AML MNC are available in the newly
diagnosed/untreated and relapsed/refractory stages.
Application
Newly Diagnosed/Untreated - AML-PB-MNC can be used
to:
• Test the efficacy of different diagnostic tools
(e.g. cellular morphology, immunocytometry, and
cytogenetics).
• Analyze drug toxicity of non-leukemia cells.
• Research interactions between leukemia and healthy
cells.
• Study the use of healthy immune cells in combination
therapies.
Relapsed/Refractory - AML-PB-MNC can be used:
• In pre-clinical studies for targeted therapies, such as
farnesyl transferase inhibitors and multidrug-resistance
protein (MDR1) inhibitors, and monoclonal antibodylinked cytotoxic agents. Currently, arsenic trioxide and
ATRA (all-trans retinoic acid) are used for relapsed
patients with AML subtype M3 (acute promyelocytic
leukemia).
• As a source to test the efficacy of current and future
diagnostic tools.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Related Products
AML-BM-MNC
NPB-MNC
Published Papers
Hart, S., et al. (2011). Pacritinib (SB1518), a JAK2/FLT3 inhibitor
for the treatment of acute myeloid leukemia. Blood Cancer Journal,
1(e44). http://www.allcells.com/publications/0161allcells.pdf
This image was originally published in ASH Image Bank.
Peter Maslak. Megakaryocyte-peripheral blood - 1. ASH Image Bank. 2011; 2011-3533. The
American Society of Hematology.
www.allcells.com
For In Vitro Research Use Only
122
Diseased Cells—AML—Bone Marrow—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Newly Diagnosed/Untreated)
AML-BM015F
5 ml
$195
Frozen (Relapsed/Refractory)
AML-BM012F
5 ml
$195
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
AML-BM-Plasma
Product Description
Lead Time
AML-BM Plasma is obtained by centrifugation of the adult AMLBM. Plasma has a diminished number of cells and platelets.
AML plasma is available in the newly diagnosed/untreated and
relapsed/refractory stages.
Product delivery is subject to stock availability.
Related Products
AML-PB-Plasma
BM-Plasma
Application
Diseased Cells: Leukemia: Aml
AML-BM Plasma can be used to study and analyze possible
leukemia stem and progenitor cell protein secretion into the serum
plasma.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
123
Diseased Cells—AML—Bone Marrow—Mononuclear Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
AML-BM001F
10 million/vial
$647
Comprehensive RNA
RNA-AML-BM001C
5.0 µg
$583
cDNA
cDNA-AML-BM001
10 PCR rxns
$583
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
AML-BM003F
10 million/vial
$647
Comprehensive RNA
RNA-AML-BM002C
5.0 µg
$647
cDNA
cDNA-AML-BM002
10 PCR rxns
$583
Relapsed/Refractory
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
AML-BM-MNC
Diseased Cells: Leukemia: Aml
Product Description
BM is obtained from adult AML patients. BM-MNC are isolated
by diluting the bone marrow with phosphate-buffered saline and
using gradient separation techniques. After centrifugation, the MNC
layer is collected. MNC can be processed further to isolate cell
subpopulations. AML-MNC are available in the newly diagnosed/
untreated and relapsed/refractory stages.
Application
Newly Diagnosed/Untreated - AML-BM-MNC can be used to
study:
• Induction chemotherapy effects performing as general cell
suppressors of active proliferation.
• The effects of chemotherapy that involve cytarabine (Ara-C),
anthracycline, or clinical trial candidates.
• Cell cycle regulation failure of isolated BM myeloblasts.
Relapsed/Refractory - AML-MNC can be used to:
• Study clinical trial, targeted therapies, such as farnesyl
transferase inhibitors and multidrug-resistance protein (MDR1)
inhibitors, and monoclonal antibody-linked cytotoxic agents.
Currently, arsenic trioxide and ATRA (all-trans retinoic acid)
are used for relapsed AML subtype M3 (acute promyelocytic
leukemia) patients.
• Target drug resistance.
• Test small molecule and antibody drug-delivery methods.
Lead Time
Product delivery is subject to stock availability.
Related Products
AML-PB-MNC
BM-MNC
Published Papers
Hart, S., et al. (2011). Pacritinib (SB1518), a JAK2/FLT3 inhibitor
for the treatment of acute myeloid leukemia. Blood Cancer Journal,
1(e44). http://www.allcells.com/publications/0161allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
124
Diseased Cells—AML—Bone Marrow—CD34 + Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
AML-BM006F
1 million/vial
$1323
AML-BM007F
5 million/vial
$5410
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
AML-BM009F
1 million/vial
$1323
AML-BM010F
5 million/vial
$5410
Relapsed/Refractory
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
AML-BM-CD34+ Cells
Product Description
Purity
AML-BM-CD34+ cells are selected from adult AML-BM-MNC. The
BM-CD34+ stem/progenitor cells are positively isolated using a
direct immunomagnetic CD34 MicroBead labeling system. AMLCD34+ cells are available in the newly diagnosed/untreated and
relapsed/refractory stages.
Toll Free Phone: 1-888-535-3444
Diseased Cells: Leukemia: Aml
AML-BM-CD34+ cells can be used to:
• Function as a potential source of leukemia stem cells.
• Study loss of cell cycle regulatory mechanisms and
uncontrolled cell proliferation.
• Research novel genetic mutations, such as in Flt-3, for
subtyping and therapeutic research.
ALLCELLS
Lead Time
Product delivery is subject to stock availability.
Application
The purity is guaranteed to be at least 90% as assessed by flow
cytometric analysis.
Related Products
BM-CD34+ Cells
AML-PB-CD34+ Cells
www.allcells.com
For In Vitro Research Use Only
125
Diseased Cells—Myelodysplastic Syndrome (MDS)—Bone Marrow—
Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Newly Diagnosed/Untreated)
MDS-BM001F
10 million/vial
$622
Frozen (Relapsed/Refractory)
MDS-BM003F
10 million/vial
$622
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MDS-BM-MNC
Product Description
Background
AllCells has isolated mononuclear cells (MNCs) from the bone
marrow of Myelodysplastic Syndrome (MDS) patients. Patients
participating in our MDS donor program have been characterized as
either Newly Diagnosed/Untreated or Relapsed/Refractory. Medical history of each patient is available which may include treatment
history, serum biochemistry, bone marrow aspirate smear analysis,
cytogenics, and immunophenotyping.
Frozen MDS BM MNC - BM is obtained from adult Myelodysplastic
Syndrome patients. BM-MNC are isolated by diluting the bone marrow with phosphate-buffered saline and using gradient separation
techniques. After centrifugation,the MNC layer is collected. MNC
can be processed further to isolate cell subpopulations.
Related Products
AML-BM-MNC
Lead Time
Diseased Cells: Leukemia: MDS
The myelodysplastic syndromes (MDS, formerly known as “preleukemia”) are a diverse collection of hematological conditions united
by ineffective production (dysplasia) of myeloid blood cells; these
conditions risk transformation to acute myelogenous leukemia
(AML). Myelodysplastic syndromes are bone marrow stem cell
disorders resulting in disorderly and ineffective hematopoiesis
manifested by irreversible quantitative and qualitative defects in
hematopoietic cells. In a majority of cases, the course of disease
is chronic with gradually worsening cytopenias due to progressive
bone marrow failure. Approximately one-third of patients with MDS
progress to AML within months to a few years.
Product delivery is subject to stock availability.
This image was originally published in ASH Image Bank.
Peter Maslak. Post-BMT aspirate - 1. ASH Image Bank. 2011; 2011-3540. The American
Society of Hematology.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
126
Chronic Lymphoid Leukemia
Diseased Cells—CLL—Peripheral Blood—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Newly Diagnosed/Untreated)
CLL-PB011F
5 ml
$191
Frozen (Relapsed/Refractory)
CLL-PB012F
5 ml
$191
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CLL-PB-Plasma
Lead Time
Product Description
CLL-PB Plasma is obtained by centrifugation of the CLL-PB-MNC.
Plasma has a diminished number of cells and platelets. CLL plasma
is available in newly diagnosed/untreated and relapsed/refractory
stages.
Application
Product delivery is subject to stock availability.
Related Products
CLL-BM-Plasma
NPB-Plasma
Newly Diagnosed/Untreated - CLL-PB Plasma can be used to study
the cell secretion and protein analysis into the serum or plasma.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Diseased Cells: Leukemia: CLL
Relapsed/Refractory - CLL-PB Plasma can be used to study:
• Cell secretion that might elucidate drug resistance mechanisms.
• The drug delivery efficiency and drug turnover rate if there a
drug treatment history exists.
www.allcells.com
For In Vitro Research Use Only
127
Diseased Cells—CLL—Peripheral Blood— Mononuclear Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-PB001F
10 million/vial
$635
CLL-PB002F
50 million/vial
$1523
cDNA-CLL-PB001
10 PCR rxns
$572
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-PB003F
10 million/vial
$635
CLL-PB007F
50 million/vial
$1523
cDNA-CLL-PB002
10 PCR rxns
$572
cDNA
Relapsed/Refractory
cDNA
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CLL-PB-MNC
Diseased Cells: Leukemia: CLL
Product Description
CLL-PB-MNC are isolated from CLL-peripheral blood by diluting
the blood with phosphate-buffered saline and using gradient separation. MNC can be processed to isolate subpopulations. CLL-PBMNC are available in newly diagnosed/untreated and relapsed/
refractory stages.
Application
Lead Time
Product delivery is subject to stock availability.
Related Products
CML-PB-MNC
NPB-MNC
Newly Diagnosed/Untreated - CLL-PB-MNC can be used to:
• Research improved methods for diagnosis and prognosis
from early to late CLL stages.
• Study B lymphocytosis effects on other cells, which cause
problems, such as cytoreduction.
• Establish markers to distinguish B-CLL from similar diseases.
• Test immunosuppressive and chemotherapeutic drugs that
involve purine analogs and monoclonal antibodies.
• Examine the capacity to generate cellular immune response
to newly established CLL-associated antigens.
Relapsed/Refractory - CLL-PB-MNC can be used to study:
• B lymphocyte resistance to primary treatments
• Therapeutic drugs involving stronger purine analog and
monoclonal antibodies
This image was originally published in ASH Image Bank.
Peter Maslak. Smudge cells - 1. ASH Image Bank. 2011; 2011-1023. Peripheral blood smear
from a patient with CLL shows lymphocytes as well as “smudge” cells. The American Society
of Hematology.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
128
Diseased Cells—CLL—Peripheral Blood—CD19 +/CD5 + B Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-B013F
1 million/vial
$572
CLL-B014F
5 million/vial
$1485
cDNA-CLL-B005
10 PCR rxns
$623
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-B019F
1 million/vial
$572
CLL-B020F
5 million/vial
$1485
cDNA-CLL-B006
10 PCR rxns
$623
cDNA
Relapsed/Refractory
cDNA
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CLL-PB-CD19+/CD5+ B Cells
Application
CLL-PB-CD19+/CD5+ B cells are isolated from CLL peripheral
blood mononuclear cells using a direct immunomagnetic CD19+
MicroBead labeling system. Available in newly diagnosed/untreated or relapsed/refractory stages.
The co-expression of CD5 is typically found on cancerous CLLCD19+ B cells. Current research is underway to identify the CLL
clonal origin of CLL from this cell population. Although these
cells appear mature in morphology, they are in developmental
arrest. Researchers have shown that only 1% of these cells are
in an active cycle. CD19+/CD5+ B cells play an important role in
production of IgM autoantibodies.
Newly Diagnosed/Untreated - These cells are a good target to
research CLL-related autoimmune complications.
Relapsed/Refractory - CLL-PB-CD19+/CD5+ B cells can be used
to find strategies against drug resistance.
Purity
The purity is guaranteed to be at least 90% as assessed by flow
cytometric analysis.
Lead Time
Related Products
CLL-BM-CD2+ T Cells CLL-BM-CD19+ Cells
Product
delivery is+ Cells
subject to stockNormal
availability.
CLL-PB-CD19
NPB-CD19+ Cells
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Diseased Cells: Leukemia: CLL
Product Description
129
Diseased Cells—CLL—Bone Marrow—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Newly Diagnosed/Untreated)
CLL-BM011F
5 ml
$191
Frozen (Relapsed/Refractory)
CLL-BM012F
5 ml
$191
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CLL-BM-Plasma
Lead Time
Product Description
CLL BM-Plasma is obtained by centrifugation of the CLL bone
marrow. Plasma has a diminished number of cells and platelets. CLL
plasma is available in newly diagnosed/untreated and relapsed/
refractory stages.
Application
Product delivery is subject to stock availability.
Related Products
CLL-PB-Plasma
BM-Plasma
Newly Diagnosed/Untreated - CLL-BM-Plasma can be used to:
• Study extracellular proteins that could play a role in the cause
and progression of the CLL-B cell malignancies.
• Function as part of diagnostic and prognostic tools.
Diseased Cells: Leukemia: CLL
Relapsed/Refractory - CLL-BM-Plasma can be used to:
• Research the role of the CLL-BM micro-environment.
• Analyze leukemia cell secretion into bone marrow serum
or plasma.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
130
Diseased Cells—CLL—Bone Marrow—Mononuclear Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-BM001F
10 million/vial
$635
cDNA
cDNA-CLL-BM001
10 PCR rxns
$572
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-BM003F
10 million/vial
$635
cDNA
cDNA-CLL-BM002
10 PCR rxns
$572
Relapsed/Refractory
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CLL-BM-MNC
Lead Time
Product Description
CLL-BM-MNC are isolated by diluting CML bone marrow
with phosphate-buffered saline and using gradient separation
techniques. After centrifugation, the MNC layer is collected. CLLBM-MNC can be processed further to isolate subpopulations.
They are available in either newly diagnosed/untreated or
relapsed/refractory stages.
Product delivery is subject to stock availability.
Related Products
CLL-BM-MNC
BM-MNC
Diseased Cells: Leukemia: CLL
Application
Newly Diagnosed/Untreated - CLL-BM-MNC can be used to:
• Research more improved methods for diagnosis and
prognosis from early to late CLL stages.
• Study B lymphocytosis effects on other cells, such as
cytoreduction.
• Establish markers to distinguish B-CLL from similar
diseases.
• Test drugs that involve purine analogs and monoclonal
antibodies.
• Examine the capacity to generate cellular immune response
to newly established CLL-associated antigens.
Relapsed/Refractory - CLL-BM-MNC can be used to study:
• B lymphocyte resistance to primary treatments
• Therapeutic drugs that involve stronger purine analog and
monoclonal antibodies
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
131
Diseased Cells—CLL—Bone Marrow—CD19 +/CD5 + B Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-B037F
1 million/vial
$635
CLL-B038F
5 million/vial
$1775
cDNA-CLL-B009
10 PCR rxns
$666
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-B043F
1 million/vial
$635
CLL-B044F
5 million/vial
$1775
cDNA-CLL-B010
10 PCR rxns
$666
cDNA
Relapsed/Refractory
cDNA
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CLL-BM-CD19+/CD5+ B Cells
Purity
Product Description
CLL-PB-CD19+/CD5+ B cells are isolated from CLL bone marrow
mononuclear cells using a direct immunomagnetic CD19+ MicroBead labeling system. Available in newly diagnosed/untreated or
relapsed/refractory stages.
The purity is guaranteed to be at least 90% as assessed by flow
cytometric analysis.
CD5 co-expression on BM-CD19+ B Cells can be used as a strong
indicator of CLL infiltration in the bone marrow. In many untreated
or lower-staged CLL cases, the co-expression level of CD5 in
BM-CD19+ cells is significantly lower than the co-expression level
within peripheral blood.
Product delivery is subject to stock availability.
Lead Time
Application
Newly Diagnosed/Untreated - These cells can be used to study
the transition from the mantle zone to the bone marrow microenvironment.
Relapsed/Refractory - CLL-BM-CD19+/CD5+ B Cells can be
used to study cellular and molecular mechanisms that cells use
to prolong survival and expansion. These cells can also be used
to research prognosis and prevention methods of acute/blastic
transformation.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
132
Chronic Myeloid Leukemia
Diseased Cells—CML—Peripheral Blood—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Chronic Phase)
CML-PB011F
5 ml
$191
Frozen
(Accelerated/Blast Crisis)
CML-PB012F
5 ml
$191
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CML-PB-Plasma
Lead Time
Product Description
CML-PB Plasma is obtained by centrifuging CML peripheral blood.
Plasma has a diminished number of cells and platelets. CML
plasma is available in the chronic phase and accelerated or blast
crisis phase.
Product delivery is subject to stock availability.
Related Products
CML-BM-Plasma
Application
Diseased
Diseased Cells:
Cells: Leukemia:
Leukemia: CML
CLL
CML-PB Plasma can be used to study and analyze:
• Leukemia cell secretion in to the serum or plasma
• Other possible leukemia candidate and target proteins and
pathways in the serum or plasma
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
133
Diseased Cells—CML—Peripheral Blood—Mononuclear Cells
Chronic Phase
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CML-PB001F
10 million/vial
$1710
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CML-PB003F
10 million/vial
$1710
Accelerated or Blast Crisis
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CML-PB-MNC
Background
Application
Chronic Myeloid Leukemia (CML) is often divided into three
phases, based on clinical characteristics and laboratory findings. In
the absence of interventions, CML typically begins in the chronic
phase (85% of CML patients). Over the course of several years, the
cancer progresses to an accelerated phase and ultimately to a blast
crisis phase.
Diseased Cells: Leukemia: CML
Product Description
ALLCELLS
Accelerated/Blast Crisis - CML-PB-MNC can be used to
improve methods of diagnosis, classification, and prognosis.
Lead Time
CLL-PB-MNC are isolated from CML peripheral blood by diluting
the blood with phosphate-buffered saline and using gradient
separation techniques. After centrifugation, the MNC layer is
collected. MNC can be processed further to isolate subpopulations.
CML-PB-MNC are available in the chronic phase and accelerated
or blast crisis phase.
Chronic Phase - CML-PB-MNC can be used to improve methods of
diagnosis, classification, and prognosis. In addition, researchers can
use these cells to study leukemia cells’ ability to affect and expel
healthy cells.
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Related Products
CML-BM-MNC
NPB-MNC
www.allcells.com
For In Vitro Research Use Only
134
Diseased Cells—CML—Bone Marrow—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Chronic Phase)
CML-BM011F
5 ml
$191
Frozen
(Accelerated/Blast Crisis)
CML-BM012F
5 ml
$191
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CML-BM-Plasma
Product Description
Lead Time
CML-BM plasma is obtained by centrifugation of the CML bone
marrow. Plasma has a diminished number of cells and platelets.
CML-BM plasma is available in chronic phase and accelerated or
blast crisis phase.
Product delivery is subject to stock availability.
Related Products
CML-PB-Plasma
Application
Diseased Cells: Leukemia: CML
CML-BM-Plasma can be used to study and analyze:
• Leukemia cell secretion into the serum or plasma
• Other possible leukemia candidates, target proteins, and pathways
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
135
Diseased Cells—CML—Bone Marrow—Mononuclear Cells
Chronic Phase
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CML-BM001F
10 million/vial
$1900
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CML-BM003F
10 million/vial
$1900
Accelerated or Blast Crisis
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CML-BM-MNC
Product Description
Lead Time
CML-BM-MNC are isolated by diluting CML bone marrow
with phosphate-buffered saline and using gradient separation
techniques. After centrifugation, the MNC layer is collected. CMLMNC can be processed further to isolate subpopulations. CML-BMMNC are available in either the chronic phase or accelerated/blast
crisis phase.
Related Products
AML-PB-MNC
BM-MNC
Published Papers
Takeuchi, Miki, et al. (2010). Rakicidin A effectively induces apoptosis
in hypoxia adapted Bcr-Abl positive leukemic cells. Cancer Science,
102(3). http://www.allcells.com/publications/0158allcells.pdf
Application
Diseased Cells: Leukemia: CML
Product delivery is subject to stock availability.
Chronic Phase - CML-BM-MNC can be used to research improved
methods of diagnosis, classification, and prognosis.
Accelerated/Blast Crisis - CML-BM-MNC can be used for CML
therapeutic studies. At advanced stages, the disease regresses
to earlier stages of cells in the bone marrow, becoming more
symptomatically and characteristically like AML.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
136
Diseased Cells—CML—Bone Marrow—CD34 + Cells
Chronic Phase
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CML-BM008-1F
0.2 million/vial
$1399
CML-BM008-2F
1 million/vial
$3310
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CML-BM009-1F
0.2 million/vial
$1399
CML-BM009-2F
1 million/vial
$3310
Accelerated or Blast Crisis
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CML-BM-CD34+ Cells
Product Description
Purity
CML-BM-CD34+ Cells are isolated from the bone marrow using
a direct CD34 MicroBead labeling system. CML-BM-CD34+
Cells are available in the chronic phase and the accelerated or
blast crisis phase.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
Product delivery is subject to stock availability.
Application
Related Products
CML-BM-MNC
NPB-MNC
Diseased Cells: Leukemia: CML
Chronic Phase- CML-BM-CD34+ cells can be used to research
the origin of clonality and proliferation. These cells can be used
as part of disease diagnostic and prognostic tools. In addition,
these cells can be used to study mechanisms in relation to
potential prevention of acute or blastic phase transformation.
Accelerated/Blast Crisis - CML-BM-CD34+ Cells can be used to
elucidate cases of Ph1 negative CML that produce BCR/ABL
chimeric gene, cytogenetic relapse of CML, and relapse in cells
of donor origin.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
137
Multiple Myeloma cells & tissue
Multiple Myeloma (MM) Table of Contents
MM Peripheral Blood Plasma
138
MM Bone Marrow Plasma CD138+ Cells
141
MM Peripheral Blood Mononuclear Cells
139
MM Bone Marrow Mononuclear Cells
142
MM Bone Marrow Plasma
140
Multiple Myeloma Cells & Tissue
AllCells specializes in highly purified mononuclear cells from peripheral blood and bone marrow of multiple myeloma patients. We also
provide CD138+ myeloma plasma cells isolated from patient samples. These multiple myeloma cells are made available from newly
diagnosed or relapsed/refractory patients and can be provided in frozen, fresh formats depending on availability. Please contact us for any
other special requests.
A Certificate of Analysis (CofA) for each product will be provided with the shipment. The information about the patient includes their
age, gender, race, weight, height, classification of the disease, the treatment history, and the status of the disease when the cells were
obtained. A CD138+ myeloma plasma cell percentage is also included. The CofA also includes information about the product such as the
number of myeloma cells and the viability of the cells in the product vial.
The clinical reports for each product will be provided with the shipment. Medical history of each patient is available and may include treatment history, serum biochemistry, bone marrow aspirate smear analysis, cytogenics, and immunophenotyping.
Please note that due to the nature of the disease and sensitivity of the cancer patients, product availability is limited.
Background
Multiple Myeloma (MM) is a type of incurable cancer of the plasma cells. Normal plasma cells make antibodies that attack foreign
microorganisms and viruses in the body. MM occurs when these plasma cells grow out of control, becoming malignant and form a tumor in
the bone marrow. MM has several types of characterizations. An excessive amount of plasma cells can cause problems in the bone marrow
where blood cells are made. Consequently, the bone marrow may not be able to make enough red blood cells, platelets, or normal white
blood cells to sustain normal body functions.
For more information visit: www.allcells.com/multiple-myeloma/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
138
Diseased Cells—MM—Peripheral Blood—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Newly Diagnosed/Untreated)
MM-PB011F
5 ml
$256
Frozen (Relapsed/Refractory)
MM-PB012F
5 ml
$256
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MM-PB-Plasma
Product Description
Lead Time
MM-PB-Plasma is obtained by centrifugation of MM peripheral
blood. Plasma has a diminished number of cells. MM-PB-Plasma
is available in both the newly diagnosed/untreated and relapsed/
refractory stages.
Product delivery is subject to stock availability.
Related Products
NPB-Plasma
MM-MM-Plasma
Application
Newly Diagnosed/Untreated - MM-PB-Plasma can be used to:
• Study production and manifestation of free serum or urine
immunoglobulin, or paraprotein. These paraproteins are
indicators of disease stages.
• As a source for better diagnostic and prognostic tools.
Diseased Cells: MM
Relapsed/Refractory - MM-PB-Plasma can be used as:
• A source for paraprotein analysis
• A staging tool
• Insight into cellular interactions and pre or post therapeutic
effects
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
139
Diseased Cells—MM—Peripheral Blood—Mononuclear Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MM-PB001F
10 million/vial
$705
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MM-PB003F
10 million/vial
$705
Relapsed/Refractory
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MM-PB-MNC
Product Description
Lead Time
MM-PB-MNC are isolated from MM-peripheral blood by diluting the
blood with phosphate-buffered saline and using gradient separation
techniques. After centrifugation, the MNC layer is collected. MNC
can be processed further to isolate subpopulations. MM-PB-MNC
products are available in both the newly diagnosed/untreated and
the relapsed/refractory stages.
Product delivery is subject to stock availability.
Related Products
BM-MNC
MM-BM-MNC
Diseased Cells: MM
Application
Newly Diagnosed/Untreated - MM-PB-MNC can be used to:
• Test for better tools of diagnosis and prognosis.
• Find more efficient ways of diagnosing effects from specific
translocations, such as FGFR3 over expression.
• Evaluate the effect on normal healthy cells from the amplified
cloning of plasma cells and immunoglobulins.
• Evaluate symptoms, such as hypercalcemia.
Relapsed/Refractory - MM-PB-MNC can be used to:
• Screen in vitro therapeutic methods.
• Profile cells at relapsed or refractory stages.
• Determine the cellular effects of therapeutic drugs.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
140
Diseased Cells—MM—Bone Marrow—Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Newly Diagnosed/Untreated)
MM-BM011F
5 ml
$256
Frozen (Relapsed/Refractory)
MM-BM012F
5 ml
$256
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MM-BM-Plasma
Lead Time
Product Description
Product delivery is subject to stock availability.
MM-BM Plasma is obtained by centrifuging the MM-bone marrow.
The plasma has a diminished number of cells and platelets. MMBM-plasma is available in both the newly diagnosed/untreated and
relapsed/refractory stages.
Related Products
MM-PB-Plasma
NPB-Plasma
Application
Newly Diagnosed/Untreated - MM-BM-Plasma can be used to study:
• Plasma cell secretion into the bone marrow serum/plasma.
• Immunoglobulin/paraprotein prognostic tools.
• The effect of plasma cell overproliferation on healthy cells.
• Bone deterioration and lesions (by detecting of hypercalcemia).
• Hyperviscosity syndrome therapies.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Diseased Cells: MM
Relapsed/Refractory - MM-BM-Plasma can be used to:
• Study cell secretion and paraproteins, which can help improve
sub-typing and staging systems.
• Analyze calcium levels as diagnostic indicators of bone lesion
severity.
www.allcells.com
For In Vitro Research Use Only
141
Diseased Cells—MM—Bone Marrow—CD138 + Plasma Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Frozen
MM-BM004F
1 million/vial
MM-BM005F
5 million/vial
MM-BM006F
10 million/vial
Product Format
Catalog #
QTY/Unit
Frozen
MM-BM013F
1 million/vial
MM-BM014F
5 million/vial
MM-BM015F
10 million/vial
Price/Unit
Inquire for availability
Relapsed/Refractory
Price/Unit
Inquire for availability
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MM-BM-CD138+ Plasma Cells
Viability
Product Description
MM-BM-CD138+ Plasma cells are positively isolated from MNC
using a direct immunomagnetic CD138 MicroBead labeling system. MM-BM-CD138+ plasma cells may be available as custom
products only. Please contact us for more information.
Diseased Cells: MM
Application
Newly Diagnosed/Untreated - MM-BM-CD138+ Plasma cells can
be used to study:
• Oncogenic mutations and translocations
• Plasma cell production
• Proliferation mechanisms that involve immunoglobulin to
suppress drug treatments
• Potential sources to sort myeloma stem cells
The viability ranges from 50% to 75%.
Product Description
MM-BM Plasma is obtained by centrifuging the MM-bone marrow.
The plasma has a diminished number of cells and platelets. MMBM-plasma is available in both the newly diagnosed/untreated
and relapsed/refractory stages.
Related Products
BM-CD138+ Plasma Cells
Relapsed/Refractory - MM-BM-CD138+ Plasma cells can be used
to research:
• Containment and suppression strategies against resistance
to primary treatments by re-treating cells, using the original
agents in stronger doses, either alone or in combination
• Drug-resistant cell populations that give rise to malignant cells
and myeloma in the bone marrow
Purity
The purity is guaranteed to be at least 75% as assessed by flow
cytometric analysis.
This image was originally published in ASH Image Bank.
Thorsten Austein. Four-nucleate plasma cell in multiple myeloma - 1. ASH Image Bank. 2011;
2011-1133. The American Society of Hematology.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
142
Diseased Cells—MM—Bone Marrow—Mononuclear Cells
Newly Diagnosed/Untreated
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MM-BM001F
10 million/vial
$979
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MM-BM003F
10 million/vial
$768
Relapsed/Refractory
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MM-BM-MNC
Lead Time
Product Description
MM-BM-MNC are isolated by diluting MM-bone marrow with
phosphate-buffered saline and using gradient separation. MM-BMMNC products are available in both the newly diagnosed/untreated
and relapsed/refractory stages.
Product delivery is subject to stock availability.
Related Products
BM-MNC
MM-PB-MNC
Application
Diseased Cells: MM
Newly Diagnosed/Untreated - MM-BM-MNC can be used to study:
• Effects of plasma cells and paraproteins on the surrounding
healthy cells and the environment
• MM stem cell resistance to therapeutic methods
• Plasma cell percentage and cell surface expression within
bone marrow cells
Relapsed/Refractory - MM-BM-MNC can be used to research:
• Prevention methods for MM associated symptoms, such as
osteoporosis, hypercalcemia, anemia, and local angiogenesis
• Disease containment and suppression therapies
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
143
Autoimmune cells & tissue
Autoimmune Table of Contents
Lupus (SLE)144
Rheumatoid Arthritis (RA)
148
Multiple Sclerosis (MS)
Type I Diabetes (DIA1)
149
Type II Diabetes (DIA2)
150
145
Osteoarthritis (OA)146
Psoriasis (PSO)147
Autoimmune Cells & Tissue
AllCells provides patient samples of Autoimmune cells isolated from Rheumatoid Arthritis, , Osteoarthritis, Systemic Lupus Erythematosus
(SLE), Psoriasis, Type I & Type II Diabetic, and Multiple Sclerosis patients. Autoimmune plasma and autoimmune peripheral blood mononuclear cells are available from each disease type. These are available in frozen or fresh formats upon request.
Patient treatment history and clinical reports are available for each sample. This may include auto antibody analysis or clinical diagnostic
scores (ACR, SLEDAI, etc).
Donor Criteria & Screening
Autoimmune cells and tissue products are obtained from volunteers participating in an Institutional Review Board (IRB) or Human Subject
Committee approved donor program. All donors sign an informed consent form and are aware that these cells are used for research purposes. Donors must be over the age of 18.
Background
Autoimmune Diseases arise from an inappropriate immune response of the body against substances and tissues normally
present within the body. This disease may be restricted to certain organs or involve a particular tissue located in the body.
Treatment of Autoimmune Diseases is typically with immunosuppression or medication which decreases the immune response.
For a disease to be classified as an Autoimmune Disease there must be direct evidence from transfer of pathogenic antibodies or pathogenic T cells, indirect evidence based on reproduction of the autoimmune disease in experimental animals,
or circumstantial evidence from clinical clues.
Autoimmune specimens can be used in preclinical research, drug discovery, drug toxicity, biomarker development, and preselection of indication for clinical trials.
For more information visit: www.allcells.com/autoimmune/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
144
Diseased Cells—Lupus (SLE)—Peripheral Blood/Plasma/Mononuclear
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
SLE-WB40
40 ml
Inquire for pricing
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
SLE-PB011F
5 ml
$199
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
SLE-PB001F
10 million/vial
$499
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Application
AllCells has isolated mononuclear cells from the peripheral blood of
Systemic Lupus Erythematosus (SLE) patients. In addition plasma
collects are also available. Medical history of each patient is available and may include treatment history, ANA analysis, and SLEDAI
score.
Fresh SLE WB - Whole blood from Systemic Lupus Erythematosus
patients is anti-coagulated in 5x10ml heparin tubes.
Frozen SLE PB Plasma - SLE peripheral blood plasma is obtained
by centrifugation of the SLE PB. This product has a diminished
number of cells and platelets. Fresh SLE PB plasma is available
upon request.
Frozen SLE PB MNC - Peripheral blood mononuclear cells are
isolated from SLE peripheral blood by diluting the whole blood with
PBS and using gradient separation techniques. After centrifugation, the mononuclear cell layer is collected. MNC can be processed
further to isolate subpopulations. Fresh SLE PB MNC is available
upon request.
Lead Time
SLE specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
Background
Systemic Lupus Erythematosus is a Type III Hypersensitivity Reaction caused by an antibody immune complex formation. Often
referred to as 'lupus', SLE is a systemic autoimmune disease that
can affect any part of the body.
SLE is treatable using immunosuppression, mainly with cyclophosphamide, corticosteroids, and other immunosuppressants. There is
currently no cure for SLE. SLE can be fatal although with recent
medical advances, fatalities are becoming increasingly rare. Survival
for people with SLE in the United States and Canada has risen to
approximately 95% at 5 years, 90% at 10 years, and 78% at 20
years.
Product delivery is subject to stock availability.
Antinuclear antibody testing (ANA) and anti-extractable nuclear
antigen (anti-ENA) are the mainstay of serologic testing for SLE.
Other tests routinely performed in suspected SLE diagnosis are
compliment system levels, electrolytes and renal function, liver
enzymes, and complete blood count.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Diseased Cells: Autoimmune: SLE
Product Description
145
Diseased Cells—Multiple Sclerosis (MS)—Peripheral Blood/Plasma/
Mononuclear Cells
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MS-PB011F
5 ml
$199
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MS-PB001F
10 million/vial
$499
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Application
Diseased Cells: Autoimmune: MS
AllCells has isolated mononuclear cells from the peripheral blood of
MS patients. In addition plasma collections are also available. Treatment history of each patient is available.
Frozen MS PB Plasma - MS peripheral blood plasma is obtained by
centrifugation of the MS PB. This product has a diminished number
of cells and platelets. Fresh MS PB plasma is available upon request.
Frozen MS PB MNC - Peripheral blood mononuclear cells are
isolated from MS peripheral blood by diluting the whole blood with
PBS and using gradient separation techniques. After centrifugation, the mononuclear cell layer is collected. MNC can be processed
further to isolate subpopulations. Fresh MS PB MNC is available
upon request.
MS specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
Background
Multiple Sclerosis also known as (Disseminated Sclerosis or Encephalomyelitis Disseminata) is an inflammatory disease in which
the fatty myelin sheaths around the axons of the brain and spinal
cord are damaged leading to demyelination and scarring.
The disease usually occurs in young adults and is more common in
women than in men. In MS the body’s own immune system attacks
the myelin. When myelin is lost, the axons can no longer effectively
conduct electrical signals.
Lead Time
Product delivery is subject to stock availability.
Most likely MS occurs as a result of some combination of genetic,
environmental, and infectious factors. Epidemiological studies of MS
have provided hints on possible causes for the disease however the
exact cause of the disease is still unknown.
Multiple Sclerosis specimens can be used in preclinical research,
drug discovery, drug toxicity, biomarker development, and pre-selection of indication for clinical trials.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
146
Diseased Cells—Osteoarthritis (OA)—Peripheral Blood/Plasma/
Mononuclear Cells
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
OA-WB40
40 ml
Inquire for pricing
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
OA-PB011F
5 ml
$199
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
OA-PB001F
10 million/vial
$499
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Application
Fresh OA WB - Whole blood from Osteoarthritis patients is anticoagulated in 5x10ml heparin tubes.
Frozen OA PB Plasma - Osteoarthritis peripheral blood plasma is
obtained by centrifugation of the Osteoarthritis PB. This product has
a diminished number of cells and platelets. Fresh Osteoarthritis PB
plasma is available upon request.
Frozen OA PB MNC - Peripheral blood mononuclear cells are
isolated from Osteoarthritis peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation,the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Osteoarthritis PB
MNC is available upon request.
Lead Time
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Osteoarthritis specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
Background
Osteoarthritis also known as Degenerative Arthritis or Degenerative Joint Disease, is a group of mechanical abnormalities involving
degradation of joints, including articular cartilage and subchondral
bone. A variety of causes (heredity, developmental, metabolic, and
mechanical) may initiate processes leading to a loss of cartilage.
Treatment generally involves a combination of exercise, lifestyle
modification, and analgesics. OA is the most common form of
arthritis and the leading cause of chronic disability in the United
States, affecting 27 million people. Osteoarthritis is a highly-studied
indication within the therapeutic area of Inflammatory Diseases. OA
specimens can be used in preclinical research, drug discovery, drug
toxicity, biomarker development, and pre-selection of indication for
clinical trials.
www.allcells.com
For In Vitro Research Use Only
Diseased Cells: Autoimmune: OA
Newly added to our Autoimmune Cells line, mononuclear cells isolated from peripheral blood of Osteoarthritis (OA) patients are now
available. Plasma and peripheral blood are also in stock. Medical history of each patient is available and may include treatment history.
147
Diseased Cells—Psoriasis (PSO)—Peripheral Blood/Plasma/
Mononuclear Cells
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PSO-WB40
40 ml
Inquire for pricing
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
PSO-PB011F
5 ml
$199
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
PSO-PB001F
10 million/vial
$499
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Application
Diseased Cells: Autoimmune: PSO
AllCells has isolated mononuclear cells from the peripheral blood
of Psoriasis (PSO) patients. In addition plasma collections are also
available. Treatment history of each patient is available.
Fresh PSO WB - Whole blood from Psoriasis patients is anti-coagulated in 5x10ml heparin tubes.
Frozen PSO PB Plasma - Psoriasis peripheral blood plasma is
obtained by centrifugation of the Psoriasis PB. This product has a
diminished number of cells and platelets. Fresh Psoriasis PB plasma
is available upon request.
Frozen PSO PB MNC - Peripheral blood mononuclear cells are
isolated from Psoriasis peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation,the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Psoriasis PB
MNC is available upon request.
Lead Time
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Psoriasis specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
Background
Psoriasis is an autoimmune disease that appears on the skin and
occurs when epidermal cells cause an immune response that in turn
speeds up the growth cycle of skin cells. Psoriasis has been linked
to an increased risk of stroke.
The disorder is a chronic recurring condition that varies in severity
from minor localized patches to complete body coverage. Psoriasis
can also cause inflammation of the joints which is known as psoriatic arthritis. Between 10-30% of all people with psoriasis experience psoriatic arthritis.
The cause of psoriasis is not fully understood but it is believed to
have a genetic component. Various environmental factors have been
suggested as aggravating to psoriasis including stress and withdrawal of systemic corticosteroids. Psoriasis has a large hereditary
component involving many genes however it is unclear how these
genes work together. Many involve the immune system, particularly
the major histocompatibility complex (MHC) and T cells.
www.allcells.com
For In Vitro Research Use Only
148
Diseased Cells—Rheumatoid Arthritis (RA)—Peripheral Blood/Plasma/
Mononuclear Cells
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RA-PB011F
5 ml
Inquire for pricing
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RA-PB011F
5 ml
$199
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RA-PB001F
10 million/vial
$499
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Application
Fresh RA WB - Whole blood from Rheumatoid Arthritis patients is
anti-coagulated in 5x10ml heparin tubes.
Frozen RA PB Plasma - RA peripheral blood plasma is obtained
by centrifugation of the RA PB. This product has a diminished
number of cells and platelets. Fresh RA PB plasma is available upon
request.
Frozen RA PB MNC - Peripheral blood mononuclear cells are
isolated from Rheumatoid Arthritis peripheral blood by diluting the
whole blood with PBS and using gradient separation techniques.
After centrifugation, the mononuclear cell layer is collected. MNC
can be processed further to isolate subpopulations. Fresh RA PB
MNC is available upon request.
Lead Time
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
OA specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
Background
Rheumatoid Arthritis is a chronic inflammatory disorder affecting
many tissues and organs but principally attacks the synovial joints.
About 1% of the world’s population is afflicted by rheumatoid
arthritis; women 3x more often than men. Onset is most frequent
between the ages of 30-40 but people of any age can be affected.
When RA is clinically suspected, tests for the presence of rheumatoid factor (RF) are conducted. A negative RF does not rule out a
positive diagnosis; rather the arthritis is characterized as seronegative. This is the case in about 15% of patients.
Rheumatoid Arthritis is a highly-studied indication within the therapeutic area of Inflammatory Diseases. RA specimens can be used in
preclinical research, drug discovery, drug toxicity, biomarker development, and pre-selection of indication for clinical trials.
www.allcells.com
For In Vitro Research Use Only
Diseased Cells: Autoimmune: RA
AllCells has isolated mononuclear cells from the peripheral blood of
Rheumatoid Arthritis (RA) patients. In addition plasma collections
are also available. Medical history of each patient is available and
may include treatment history, RF analysis, and ACR score.
149
Diseased Cells—Type I Diabetes (DIA1)—Peripheral Blood/Plasma/
Mononuclear Cells
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
DIA1-WB40
40 ml
Inquire for pricing
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
DIA1-PB011F
5 ml
$199
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
DIA1-PB001F
10 million/vial
$499
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Application
Diseased Cells: Autoimmune: DIA1
AllCells has isolated mononuclear cells from the peripheral blood of
Type I Diabetic patients. In addition plasma collections are also available. Treatment history of each patient is available.
Fresh DIA1 WB - Whole blood from Type I Diabetic patients is anticoagulated in 5x10ml heparin tubes.
Frozen DIA1 PB Plasma - Type I Diabetes peripheral blood plasma
is obtained by centrifugation of the Type I Diabetes PB. This product
has a diminished number of cells and platelets. Fresh Diabetic Type I
PB plasma is available upon request.
Frozen DIA1 PB MNC - Peripheral blood mononuclear cells are
isolated from Type I Diabetes peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation, the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Diabetic Type I
PB MNC is available upon request.
Lead Time
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
DIA1 specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
Background
Diabetes Type I also referred to as Diabetes Mellitus Type I (formally
juvenile diabetes), results from autoimmune destruction of insulinproducing beta cells of the pancreas. The subsequent lack of insulin
leads to an increase of blood and urine glucose.
Eventually Type I Diabetes is fatal unless treated with insulin. Pancreatic transplants have been used to replenish a natural supply of
insulin. Pancreatic islet cell transplantation is experimental though
more procedures are occurring annually.
Type I Diabetes is a polygenic disease. Depending on locus or
combination of loci, it can be dominant, recessive, or somewhere inbetween. The strongest gene, IDDM1, is located in the MHC Class
II region on chromosome 6 at staining region 6p21. The appearance
of Diabetes-related autoantibodies has been shown to be able to
predict the appearance of Diabetes Type I before any hyperglycemia
occurs.
www.allcells.com
For In Vitro Research Use Only
150
Diseased Cells—Type II Diabetes (DIA2)—Peripheral Blood/Plasma/
Mononuclear Cells
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
DIA2-WB40
40 ml
Inquire for pricing
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
DIA2-PB011F
5 ml
$199
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
DIA2-PB001F
10 million/vial
$499
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Application
Fresh DIA2 WB - Whole blood from Type II Diabetic patients is anticoagulated in 5x10ml heparin tubes.
Frozen DIA2 PB Plasma - Type II Diabetes peripheral blood plasma
is obtained by centrifugation of the Type II Diabetes PB. This product has a diminished number of cells and platelets. Fresh Diabetic
Type II PB plasma is available upon request.
Frozen DIA2 PB MNC - Peripheral blood mononuclear cells are
isolated from Type II Diabetes peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation, the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Diabetic Type II
PB MNC is available upon request.
Lead Time
Product delivery is subject to stock availability.
DIA2 specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
Background
Diabetes Type II formally known as Non-insulin Dependent Diabetes Mellitus or Adult Onset Diabetes, is a metabolic disorder that is
characterized by high blood glucose in the context of insulin resistance and relative insulin deficiency. This is in contrast to Diabetes
Mellitus Type I in which there is an absolute insulin deficiency due to
destruction of islet cells of the pancreas.
Type II Diabetes makes up about 90% of cases of Diabetes with the
other 10% due primarily to Type I Diabetes and Gestational Diabetes. Obesity is thought to be the primary cause of Type II Diabetes in
people who are genetically predisposed to the disease.
Diabetes Type II specimens can be used in preclinical research, drug
discovery, drug toxicity, biomarker development, and pre-selection of
indication for clinical trials.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Diseased Cells: Autoimmune: DIA2
AllCells has isolated mononuclear cells from the peripheral blood
of Diabetic Type II patients. In addition plasma collections are also
available. Treatment history of each patient is available.
151
Diseased Cells—Sickle Cell Anemia (SCA)—Peripheral Blood/Plasma/
Mononuclear Cells
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
SCA-FPB20
20 ml
currently unavailable
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
SCA-PB011F
5 ml
currently unavailable
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
SCA-PB001F
10 million/vial
currently unavailable
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Application
Diseased Cells: SCA
AllCells has begun carrying peripheral blood from patients afflicted
with Sickle Cell Anemia (SCA). Mononuclear cells (isolated from
peripheral blood), peripheral blood, and plasma are available. Medical history of each patient is available and may include treatment
history.
Fresh SCA PB - Peripheral blood from Sickle Cell Anemia patients
is anti-coagulated using sodium heparin. Fresh Sickle Cell Anemia
PB is available in 20 ml volume.
Frozen SCA PB Plasma - Sickle Cell Anemia peripheral blood plasma is obtained by centrifugation of the SCA PB. This product has a
diminished number of cells and platelets. Fresh SCA PB plasma is
available upon request.
Frozen SCA PB MNC - Peripheral blood mononuclear cells are isolated from Sickle Cell Anemia peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation, the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh SCA PB MNC is
available upon request.
Sickle Cell Anemia is a highly-studied indication of genetic disease.
Specimens can be used in:
• Pre-clinical research
• Genetic studies
• Drug discovery
• Drug toxicity
• Biomarker development
Background
Sickle Cell Anemia (SCA) also known as Sickle Cell Disease, is an
autosomal recessive genetic blood disorder with overdominance
characterized by red blood cells that assume an abnormal, rigid,
sickle shape. Sickling decreases the cell’s flexibility and results in
various complications. The sickling occurs because of a mutation of
the hemoglobin gene. The prevalence of the disease in the United
States is approximately 1 in 5,000, mostly affecting Americans of
Sub-Saharan African descent according to the National Institutes
of Health. Approximately 1 in every 500 African American children
born will have Sickle Cell Anemia.
Lead Time
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
152
INFLAMMATORY BOWEL DISEASE cells & tissue
Inflammatory Bowel Disease Table of Contents
Crohn's Disease (CRO)
153
Ulcerative Colitis (UC)
154
Inflammatory Bowel Disease Cells & Tissue
AllCells sources samples of Inflammatory Bowel Disease (IBD) cells isolated from Crohn’s Disease and Ulcerative Colitis patients. IBD
plasma and peripheral blood mononuclear cells are available from each disease type. These are available in frozen, fresh formats upon
request.
Patient treatment history and clinical reports are available for each sample.
Donor Criteria & Screening
IBD cells and tissue products are obtained from volunteers participating in an Institutional Review Board (IRB) or Human Subject Committee approved donor program. All donors sign an informed consent form and are aware that these cells are used for research purposes.
Donors must be over the age of 18.
Background
Inflammatory Bowel Disease is a group of inflammatory conditions of the colon and small intestine. The major types of IBD are Crohn’s
Disease and Ulcerative Colitis. IBD typically present with extra-intestinal manifestations such as liver problems, arthritis, skin manifestations, and eye problems.
IBD may require immunosuppression to control symptoms such as prednisone, TNF inhibition, azathioprine, methotrexate, or 6-mercaptopurine. Once the inflammation is successfully controlled, the patient is switched to a lighter drug to keep the disease in remission.
IBD specimens can be used in preclinical research, drug discovery, drug toxicity, biomarker development, and pre-selection of indication
for clinical trials.
For more information visit: www.allcells.com/ibd/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
153
Diseased Cells—Crohn's Disease (CRO)—Peripheral Blood/Plasma/Mononuclear Cells
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CRO-PB011F
5 ml
$199
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CRO-PB001F
10 million/vial
$509
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Application
Diseased Cells: IBD: CRO
Our newly-added IBD diseased line includes mononuclear cells
isolated from peripheral blood of Crohn’s Disease patients. Plasma
collects and peripheral blood are also available. Medical history of
each patient is available and may include treatment history.
Frozen CRO PB Plasma - Crohn's Disease peripheral blood plasma
is obtained by centrifugation of the PB. This product has a diminished number of cells and platelets. Fresh Crohn's PB plasma is
available upon request.
Frozen CRO PB MNC - Peripheral blood mononuclear cells are
isolated from Crohn's Disease peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation,the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Crohn's PB MNC
is available upon request.
Lead Time
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
CRO specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
Background
Crohn’s Disease,also known as Regional Enteritis, is a type of Inflammatory Bowel Disease that may affect any part of the gastrointestinal tract from mouth to anus, causing a wide variety of symptoms. Primarily, Crohn’s Disease causes abdominal pain, diarrhea,
vomiting, weight loss, and may also cause complications outside the
gastrointestinal tract such as skin rashes, arthritis, inflammation of
the eye, tiredness, and lack of concentration.
There is no cure for Crohn’s Disease and remission may not be
possible or prolonged, if achieved at all. In cases where remission is
possible, relapse can be prevented and symptoms controlled with
medication, lifestyle change, and in some cases surgery. Treatment
for Crohn’s Disease only occurs when symptoms are active and
involves first treating the acute problem then maintaining remission.
www.allcells.com
For In Vitro Research Use Only
154
Diseased Cells—Ulcerative Colitis (UC)—Peripheral Blood/Plasma/Mononuclear Cells
Peripheral Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
UC-FPB05
5 ml
currently unavailable
UC-FPB10
10 ml
currently unavailable
UC-FPB30
30 ml
currently unavailable
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
UC-PB011F
5 ml
currently unavailable
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
UC-PB001F
10 million/vial
currently unavailable
Plasma
Peripheral Blood Mononuclear Cells
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Application
Fresh UC PB - Peripheral blood from Ulcerative Colitis patients is
anti-coagulated using sodium heparin. Fresh Ulcerative Colitis PB is
available in 5, 10 and 30 ml volumes.
Frozen UC PB Plasma - Ulcerative Colitis peripheral blood plasma is
obtained by centrifugation of the PB. This product has a diminished
number of cells and platelets. Fresh Ulcerative Colitis PB plasma is
available upon request.
Frozen UC PB MNC - Peripheral blood mononuclear cells are isolated from Ulcerative Colitis peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation,the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Ulcerative Colitis
PB MNC is available upon request.
Lead Time
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
UC specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
Background
Ulcerative Colitis is a form of Inflammatory Bowel Disease of the
colon that includes characteristic ulcers or open sores. The main
symptom of active disease is usually constant diarrhea mixed with
blood of gradual onset. UC is an intermittent disease with periods of
exacerbated symptoms and periods that are relatively symptom-free.
Treatment is generally required for the disease to go into remission.
Ulcerative Colitis occurs in 35-100 people for every 100,000 in the
United States.
Standard treatment for UC depends on extent of involvement and
disease severity. The goal is to induce remission initially with medications followed by the administration of maintenance medications
to prevent relapse of the disease.
www.allcells.com
For In Vitro Research Use Only
Diseased Cells: IBD: UC
AllCells has begun carrying peripheral blood from patients afflicted
with Ulcerative Colitis (UC). Mononuclear cells (isolated from peripheral blood), peripheral blood, and plasma are available. Medical history of each patient is available and may include treatment
history.
155
Animal Primary Cells & tissue
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
156
Animal Primary Cells & tissue
Animal Primary Cells Table of Contents
New Products
157
Non-Human Primate (NHP)
158
Mouse (Murine)159-163
Rat164-171
Pig (Porcine)172
Non-Human Primate Cells & Tissue
Non-human primate cells are obtained from NIH-supported primate research centers. The blood or bone marrow is taken
from live normal/untreated non-human primates as a part of the research centers’ tissue program. AllCells offers blood and
bone marrow non-human primate products from the following species: M. fascicularis, M. nemestrina, or M.mulatta. None of
the aforementioned species are endangered or threatened by extinction.
For more information visit: www.allcells.com/non-human-primate/
Mouse Cells & Tissue
The rat and mouse are great models for pre-clinical research. They are some of the smallest mammals with a great degree
of genetic homology to human, which makes them perfect candidates for efficient tissue procurement and study. AllCells
uses mouse cells from C57BL/6 mice for primary tissue and cell isolation.
For more information visit: www.allcells.com/mouse/
Rat Cells & Tissue
The rat and mouse are great models for pre-clinical research. They are some of the smallest mammals with a great degree
of genetic homology to human, which makes them perfect candidates for efficient tissue procurement and study. AllCells
uses rat cells from Sprague-Dawley (SD) rats for primary tissue and cell isolation.
For more information visit: www.allcells.com/rat/
Lysate and Cell Pellet products are available upon request.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
157
NEw PRODUCTS
Rat Cells & Tissue
AllCells now provides CD90 and Mesenchymal Stem Cells isolated from Rat Bone Marrow. The CD90+ cells are isolated
via immunomagnetic method from purified density-rich bone marrow mononuclear cells. For the MSCs, bone marrow mononuclear cells are expanded for up to 2 weeks on tissue culture plastic with MSC media. See product specifications and background on pages 165 and 166.
For more information visit: www.allcells.com/rat/
Pig Cells & Tissue - new for 2013
AllCells’ Porcine Mononuclear Cells are isolated from fresh blood of a mixed breed of pigs (Yorkshire, Hampshire, Poland China).
The mononuclear fraction is then further processed to eliminate red blood cells and platelets. See product specifications and background on page 172.
For more information visit: www.allcells.com/pig/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
158
Animal Cells—Non-Human Primate—Plasma/Mononuclear/CD34+ Stem Cells
Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen/PB-Plasma
NHP-PB001
5 ml
$80
Frozen/BM-Plasma
NHP-BM001
5 ml
$103
Peripheral Blood Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
NHP-PB002
10 million/vial
$1288
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
NHP-BM002
10 million/vial
$884
Bone Marrow Mononuclear Cells
Bone Marrow Mononuclear
CD34+ Stem/Progenitor
Cells
Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
NHP-BM004
1 million/vial
$1195
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Non—Human Primate Cells
Product Description
Related Products
Human BM-MNC
Human PB-MNC
Animal Cells: Non-Human Primate
NHP-Plasma is processed through centrifugation to diminish cells
and platelets.
NHP-PB-MNC are enriched by density separation.
NHP-BM-MNC are isolated from bone marrow by diluting the
bone marrow with phosphate-buffered saline and using gradient
separation techniques. After centrifugation, the MNC layer is
collected. MNC can be processed to isolate subpopulations.
NHP-BM-CD34+ cells are isolated from NHP bone marrow
mononuclear cells using a direct immunomagnetic CD34+
MicroBead labeling system.
Application
NHP products can be used to study:
• Non-human primate lymphocyte and monocyte functions
• Evolutionary conservation and homology between human and
non-human primate lymphocytes and monocytes
• The homology of BM-CD34+ cells between humans and
non-human primates, especially M. fascicularis (cynomolgus)
monkeys.
• The hematopoietic stem cell potential of the non-human
primate bone marrow CD34+ cells.
• Myeloid lineage differentiation and maturation
• Neutrophil transplantation potential
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
159
Animal Cells—Mouse—Bone Marrow—Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
MBM-001F
5 million/vial
$408
MBM-002F
10 million/vial
$512
Comprehensive RNA
RNA-MBM-001C
1 µg
$486
cDNA
cDNA-MBM-001
10 PCR rxns/20 µl
$594
Frozen
Molecular Products
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Mouse—Bone Marrow—MNC
Product Description
Purity
Mouse bone marrow is procured from the mouse femurs. The
mononuclear cells are enriched by density gradient separation.
The purity is guaranteed to be above 90% as assessed by
flow cytometric analysis.
Lead Time
Application
1-2 business days for frozen products. Product delivery is
subject to stock availability.
Related Products
Human BM MNC
Rat BM MNC
Animal Cells: Mouse
Mouse BM-MNC can be used:
• Directly or in culture, as the source of an animal model
for human bone marrow hematopoietic stem cell and
mesenchymal stem cell research.
• For novel hematopoietic cell marker and protein
identification as well as pathway analysis.
• As an animal model for transplantation and engraftment
studies.
• To study homology or cross-species conservation in
hematopoiesis and mesenchymal qualities among
mammals or larger group of organisms.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
160
Animal Cells—Mouse—Bone Marrow Mesenchymal Stem Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MMSC-001F
0.5 million/vial
Inquire for availability
Comprehensive RNA
RNA-MMSC-001C
1 µg
Inquire for availability
cDNA
cDNA-MMSC-001
10 PCR rxns/20 µl
Inquire for availability
Molecular Products
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Mouse—Bone Marrow—Mesenchymal Stem Cells
Lead Time
Product Description
Mouse marrow mesenchymal stem cells are cultured from
mononuclear cells. MNC are enriched from whole bone
marrow, which is procured from the bone marrow of mouse
femurs.
Custom products only. Please inquire for availability.
Properties
These MMSC have fibroblast-like morphology and express
CD105 (>90%) and CD90 (90%). MSC lack expression of
CD34, CD45, and CD11b.
Application
Related Products
Human Bone Marrow MSC
Fetal Liver MSC
Rat MSC
Animal Cells: Mouse
Mouse-BM-MSC can be used to:
• Study non-hematopoietic pluripotency potentials and
characteristics.
• Develop connective tissues, such as bone and
cartilage.
• Act as stroma and feeder cells for expansion or
differentiation of hematopoietic stem and progenitor
cells.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
161
Animal Cells—Mouse—Astrocytes
Cerebellar
Product Format
Cell Type
Catalog #
QTY/Unit
Price/Unit
Frozen
Cerebellar
MOCR-001F
0.5 million/vial
$340
Cortex
MCTX-001F
0.5 million/vial
$330
Hippocampal
MOHP-001F
0.5 million/vial
$352
Cortex
Frozen
Hippocampal
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Mouse—Astrocytes
Purity
Product Description
Different parts of the C57BL/6 mouse brain, including the cortex,
hippocampus, and cerebellum, are dissociated enzymatically and
mechanically into suspension of single cells. Next, the cells are
seeded, and the astrocytes are enriched in vitro, using specific
medium and cultural conditions.
The purity of mouse astrocytes is assessed by GFAP immunostaining, and these cells are guaranteed to reach 10 population
doublings when cultured using AllCells's recommended conditions.
Lead Time
1-2 business days for frozen products. Product delivery is subject
to stock availability.
Application
Animal Cells: Mouse
Mouse Astrocytes can be used to study:
• Astrocyte structure and function, such as calcium activation,
glutamate release and recycling
• Metabolic support of neurons
• Regulation of synaptic transmission
• Communication with oligodendrocytes
• Vasomodulation
• Astrocytes' role in the blood brain barrier
• Conservation or homology in mammals or in the greater
spectrum of organisms
• Astrocytoma and other diseases
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
162
Animal Cells—Mouse—Splenocytes
Product Format
Catalog #
QTY/Unit
Price/Unit
MSPL-001F
25 million/vial
$351
MSPL-002F
50 million/vial
$521
Comprehensive RNA
RNA-MSPL-001C
5 µg
$432
cDNA
cDNA-MSPL-001
10 PCR rxns/20 µl
$410
Frozen
Molecular Products
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Mouse—Splenocytes
Purity
Product Description
Mouse spleen is dissociated into a single cell suspension.
Next, the splenocytes are enriched by density separation.
The purity is guaranteed to be above 90%.
Lead Time
Application
Animal Cells: Mouse
Mouse Splenocytes can be used to study:
• Growth factor and cytokine release from lymphocytes as
well as monocytes and Kupffer cells.
• Function and mechanism of normal and diseased models
in the reticuloendothelial system.
• Homing and processing of red blood cells.
• Antigen-recognition conditioning and proliferation of
lymphocytes and monocytes.
• Reserve mechanisms for red blood cells and monocytes.
1-2 business days for frozen products. Product delivery is subject
to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
163
Animal Cells—Mouse—Purified Cells from Spleen
CD4+ T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MSPL-011F
5 million/vial
$1134
Comprehensive RNA
RNA-MSPL-011C
1 µg
$432
cDNA
cDNA-MSPL-011
10 PCR rxns/20 µl
$410
Frozen
MSPL-012F
5 million/vial
$964
Comprehensive RNA
RNA-MSPL-012C
1 µg
$432
cDNA
cDNA-MSPL-012
10 PCR rxns/20 µl
$410
Frozen
MSPL-013F
5 million/vial
$1087
Comprehensive RNA
RNA-MSPL-013C
1 µg
$432
cDNA
cDNA-MSPL-013
10 PCR rxns/20 µl
$410
CD8+ T Cells
CD19+ B Cells
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Mouse—Purified Cells from Spleen
Product Description
Mouse spleen is dissociated into a single cell suspension. The
splenocytes are enriched by density separation. CD4+ T cells,
CD8+T Cells, and CD19+ B Cells are isolated using specific
immunomagnetic methods.
Mouse-CD19+ B Cells can be used to study:
• B lymphopoiesis
• Rearrangement of the immunoglobulin genes
• Antibody production and to further distinguish
• Functions of B cell subsets
Purity
Application
Mouse-CD4 T Cells can be used to study:
• Regulation
• Gene rearrangement
• Antigen recognition and activation
• Growth factor and cytokine receptor studies,
• Autoimmunity
• Normal and diseased pathways
Animal Cells: Mouse
+
The purity is guaranteed to be above 90% as assessed by flow
cytometric analysis.
Lead Time
1-2 business days for frozen products. Product delivery is subject
to stock availability.
Mouse-CD8+ T Cells can be used to study:
• Self/non-self recognition
• Tumor and viral particles detection
• Cytolytic properties
• Immunosuppression for organ/tissue transplantation
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
164
Animal Cells—Rat—Bone Marrow—Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RBM-001F
5 million/vial
$351
RBM-002F
10 million/vial
$430
Comprehensive RNA
RNA-RBM-001C
1 µg
$432
cDNA
cDNA-RBM-001
10 PCR rxns/20 µl
$540
Molecular Products
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Rat—BM—MNC
Product Description
Lead Time
Rat bone marrow is procured from the rat femurs. The MNC are
subsequently enriched by density gradient separation.
Application
Related Products
BM-MNC
Animal Cells: Rat
Rat-BM-MNC can be used:
• Directly or in culture, as the source of an animal model
for human bone marrow hematopoietic stem cell and
mesenchymal stem cell research.
• For novel hematopoietic cell markers.
• For protein identification.
• For pathway analysis.
• As animal model for transplantation and engraftment studies.
• To study homology or cross-species conservation in hematopoiesis and mesenchymal qualities among mammals or larger
group of organisms
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
165
Animal Cells—Rat—Bone Marrow—Mesenchymal Stem Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RBM-005F
0.5 million/vial
$318
RBM-006F
1 million/vial
$508
RBM-007F
5 million/vial
$1754
Comprehensive RNA
RNA-RBM-005C
1 µg
$432
cDNA
cDNA-RBM-005
10 PCR rxns/20 µl
$453
Molecular Products
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Rat—BM—Mesenchymal Stem Cells
Purity
Product Description
Bone marrow is procured from rat femurs. Bone marrow mononuclear cells are expanded for up to 2 weeks on tissue culture
plastic with MSC media. Sprague-Dawley Rat MSC express CD90,
CD105 and CD73. They do not express CD45 and CD11b.
Background
Mesenchymal Stem Cells, or MSCs have long been believed to
be a collective, heterogeneous pool of cells responsible for the
replenishment of supportive and connective tissues of the body,
such as bone, cartilage, adipose, muscle, other soft tissues, as well
as the bone marrow stroma where hematopoietic stem and progenitor cells expand and thrive. Their role in the interactions with
and expansion support of hematopoietic stem cells in the bone
marrow is absolutely critical in the maintenance of the entire blood
cells system. Recent studies have begun to utilize the dynamic and
multi-potent properties of MSC in hopes to engineer them into
more diverse types of cells and tissues.
Purity is guaranteed over 90%.
Lead Time
1-2 business days for frozen products. Product delivery is
subject to stock availability.
Related Products
Human Bone Marrow MSC
Fetal Liver MSC
Mouse-MSC
Animal Cells: Rat
Application
As an animal model, Rat-BM-MSC can be used to:
• Study non-hematopoietic pluripotency potentials and
characteristics to develop connective tissues, such as
bone and cartilage.
• Act as stroma and feeder cells for expansion or
differentiation of hematopoietic stem/progenitor cells.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
166
Animal Cells—Rat—Bone Marrow—CD90 Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RBM-003F
1 million/vial
$533
Frozen
RBM-004F
5 million/vial
$1865
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Rat—Bone Marrow—CD90 Cells
Lead Time
Product Description
CD90/Thy-1 is considered a surrogate marker for various
kinds of stem cells, particularly hematopoietic stem cells.
Murine HSC falls under the CD90/Thy-1hi sub-population. In
rats CD90 is also expressed on thymocytes, recent thymic
emigrants, neurons, and other cell types.
1-2 business days for frozen products. Product delivery is subject
to stock availability.
Procedure
The CD90+ cells are isolated via immunomagnetic method
from purified density-rich bone marrow mononuclear cells.
Application
Rat-BM-CD90 can be used for:
• The study of rat hematopoiesis
• Stem cell biology
• Pluripotent studies
Related Products
Human CD34
NHP CD34
Purity
Animal Cells: Rat
Purity is guaranteed over 90%.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
167
Animal Cells—Rat—Astrocytes
Cerebellar
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RACR-001F
0.5 million/vial
$360
Comprehensive RNA
RNA-RACR-001C
0.2 µg
$540
cDNA
cDNA-RACR-001
10 PCR rxns/20 µl
$648
Frozen
RCTX-001F
0.5 million/vial
$360
Comprehensive RNA
RNA-RCTX-001C
0.2 µg
$540
cDNA
cDNA-RCTX-001
10 PCR rxns/20 µl
$648
Frozen
RAHP-001F
0.5 million/vial
$360
Comprehensive RNA
RNA-RAHP-001C
0.2 µg
$540
cDNA
cDNA-RAHP-001
10 PCR rxns/20 µl
$648
Cortex
Hippocampal
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Rat—Astrocytes
Purity
Product Description
Different parts of the rat brain, including the cortex,
hippocampus, and cerebellum, are dissociated enzymatically
and mechanically into suspension of single cells. Next, the
cells are seeded, and the astrocytes are enriched in vitro,
using specific medium and cultural conditions.
Lead Time
1-2 business days for frozen products. Product delivery is subject to
stock availability.
Application
Animal Cells: Rat
The purity of rat astrocytes is verified by GFAP immuno-staining.
The rat astrocytes are guaranteed 10 population doublings when
cultured under AllCells's recommended conditions.
Rat Astrocytes can be used:
• In animal studies of astrocyte structure and functions,
such as calcium activation, glutamate release and recycle,
communication with and metabolic support of neurons,
regulation of synaptic transmission, communication with
oligodendrocytes, vasomodulation and role in the blood
brain barrier.
• To study function conservation or homology in mammals or
larger groups of organisms.
• To research astrocytoma and other diseases.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
168
Animal Cells—Rat—Meningeal Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RMGC-001F
0.5 million/vial
$300
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Rat—Meningeal Cells
Product Description
Purity
Rat meninx is isolated surgically from the outer membrane
of the rat brain. Next, the meninx is enzymatically segregated
into single cell suspension and cultivated by a primary
culture process before harvest and cryopreservation. The rat
meningeal cells are positive for fibronectin, negative for: GFAP,
smooth muscle actin and thylol.
The purity is guaranteed to be above 90% as assessed by
flow cytometric analysis
Lead Time
1-2 business days for frozen products. Product delivery is
subject to stock availability.
Application
Animal Cells: Rat
Rat Meningeal Cells can be used to study:
• Structure and the protective and supportive functions of the
rat brain in relation to formation and maintenance of the
vasculature within the meninx.
• Diseases such as meningitis and meningioma.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
169
Animal Cells—Rat—Pancreatic Islets
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
SD-ILT-001
400 IEq
$907
SD-ILT-002
600 IEq
$1066
SD-ILT-003
800 IEq
$1225
Molecular Products
Comprehensive RNA
RNA-SD-ILT-C
1 µg
$323
cDNA
cDNA-SD-ILT
10 PCR rxns/20 µl
$432
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Rat—Pancreatic Islets
Product Description
Lead Time
Rat Pancreatic Islets are isolated from male rats weighing between
301 to 325 grams and approximately 8.5 to 9.5 weeks of age.
AllCells Rat Pancreatic Islets are measured by IEq standard. Rat
pancreatic islets purity is verified by dithizone or Newport Green,
and the viability by propidium iodide/acridine orange counter stains.
Upon request, the islets can be stabilized in overnight culture prior
to shipment.
1-2 business days for frozen products. Product delivery is subject to
stock availability.
Related Products
Human BM-CD34+ Cells
Human NPB-CD34+ Cells
Application
Animal Cells: Rat
Rat Pancreatic Islets can be used for the following:
• Diabetic studies
• GSIS Assay
• Short and long-term culture and survival experiments
• Cryopreservation studies
• Immunosupression studies for transplantation
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
170
Animal Cells—Rat—Splenocytes
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RSPL-001F
25 million/vial
$340
RSPL-002F
50 million/vial
$464
Comprehensive RNA
RNA-RSPL-001C
5 µg
$323
cDNA
cDNA-RSPL-001
10 PCR rxns/20 µl
$302
Molecular Products
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Rat—Splenocytes
Purity
Product Description
Rat spleen is mechanically dissociated into a single cell
suspension. The splenocytes are enriched by density
separation. The rat splenocytes can be used to isolate CD4+
T cells, CD8+ T Cells, and CD45R+ B Cells, using specific
immunomagnetic methods.
The purity is guaranteed to be above 90% as assessed by flow
cytometric analysis.
Lead Time
1-2 business days for frozen products. Product delivery is subject
to stock availability.
Application
Animal Cells: Rat
Rat Splenocytes can be used to study:
• Growth factor and cytokine release from lymphocytes,
monocytes, and Kupffer cells.
• Function and mechanism of normal and diseased models
in the reticuloendothelial system.
• Homing and processing of red blood cells.
• Antigen-recognition conditioning and proliferation of
lymphocytes and monocytes.
• Reserve mechanisms for red blood cells and monocytes.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
171
Animal Cells—Rat—Purified Splenocytes
CD4+ T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
RSPL-011F
5 million/vial
$862
Comprehensive RNA
RNA-RSPL-011C
1 µg
$323
cDNA
cDNA-RSPL-011
10 PCR rxns/20 µl
$302
Frozen
RSPL-012F
5 million/vial
$907
Comprehensive RNA
RNA-RSPL-012C
1 µg
$323
cDNA
cDNA-RSPL-012
10 PCR rxns/20 µl
$302
Frozen
RSPL-013F
5 million/vial
$794
Comprehensive RNA
RNA-RSPL-013C
1 µg
$323
cDNA
cNA-RSPL-013
10 PCR rxns/20 µl
$302
CD8+ T Cells
CD45R+ B Cells
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Rat—Purified Splenocytes
Product Description
Rat spleen is mechanically dissociated into a single cell
suspension. The splenocytes are enriched by density
separation. CD4+ T cells, CD8+ T Cells, and CD45R+ B Cells
and then isolated using specific immunomagnetic methods.
Animal Cells: Rat
Application
Rat-CD4+ T Cells can be used to study:
• Regulation
• Gene rearrangement
• Antigen recognition and activation
• Growth factors and cytokine receptors
• Autoimmunity
• Normal and diseased pathways
Rat-CD8+ T Cells can be used to study:
• Self/non-self recognition
• Tumor and viral particles detection
• Cytolytic properties
• Immunosuppression for organ/tissue transplantation
Rat-CD45R + B Cells can be used to study:
• B lymphopoiesis
• Rearrangement of the immunoglobulin genes
• Antibody production
• Functions of B cell subsets
Purity
The purity is guaranteed to be above 90% as assessed by flow
cytometric analysis.
Lead Time
1-2 business days for frozen products. Product delivery is subject
to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
172
Animal Cells—Pig—Peripheral Blood—Mononuclear Cells
Product Format
Frozen
Catalog #
QTY/Unit
Price/Unit
PORC-PB001F
10 million/vial
$204
PORC-PB002F
100 million/vial
$410
PORC-PB003F
200 million/vial
$616
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Pig—Peripheral Blood—MNC
Product Description
Application
AllCells’ Porcine Mononuclear Cells are isolated from fresh
blood of a mixed breed of pigs (Yorkshire, Hampshire, Poland
China). The mononuclear fraction is then further processed to
eliminate red blood cells and platelets.
Background
Pig PB-MNC can be used to:
• Isolate and study MNC subpopulations
• Study cytokines secretion curves
• Act as controls for porcine therapeutic research, involving
lymphocytes and monocytes
Lead Time
Swine have increasingly become utilized as biomedical research models in the last two decades. This increased use as
an animal model is not only a result of regulatory pressure on
other large animal species, but also because swine are recognized as a suitable animal model for human disease based
upon their comparative anatomy and physiology. Swine are
used as general surgical models of most organs and systems,
for cardiovascular research including atherosclerosis, for digestive system models, and in recent years in transplantation
and xenografic research.
These frozen products have a lead time of 1-2 business days.
Related Products
Human PB MNC
They are being explored as models in many other systems
because of the widespread availability of both domestic and
miniature breeds. Hand-in-hand with this increase in the number of swine in research, have come technical developments
in surgery, anesthesia, husbandry and handling techniques.
These technical advancements have made it easier to use
this species in research and have also improved the humane
care and use of swine by research institutions worldwide.
Animal Cells: Pig
Background Reference:
http://www.nal.usda.gov/awic/pubs/swine/swine.htm
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
173
CELL Culture & CRYOPRESERVATION media
Cell Culture & Cryopreservation Media
AllCells features basic and defined Cell Culture Media to suit your specific cell culture needs. In addition we have begun
manufacturing our own AllCells Cryopreservation Media, formulated to work best with our products. For most general research purposes, we provide an array of the most commonly-used human cell culture media. For our specific cell products,
i.e. mesenchymal stem cells (MSCs), HUVEC (Human Umbilical Vascular Endothelial Cells), AllCells features defined media
for the expansion and proliferation of these cell types. See following page for all of our Cell Culture & Cryopreservation
Medium products!
For more information visit: www.allcells.com/media/
General Cell Culture Media:
Dulbecco's Modified Eagle's Medium (DMEM-001)
Dulbecco's Modified Eagle's Medium (DMEM-002)
Iscove's Modification of DMEM (IMDM-001)
Roswell Park Memorial Institute (RPMI-001)
Defined Cell Culture Media:
Fetal Liver:
Basal Medium Fetal Liver Cells MSC, 450 ml (FL-002)
Mesenchymal Stimulatory Supplement Fetal Liver Cells MSC, 50ml (FL003)
MSC Stimulatory Supplement BM MSC, 50ml (MSC-003)
Macrophage:
Macrophage Basal Medium for Normal PB Monocyte Derived Macrophages, 450 mL (PB-MDM-002)
Macrophage Stimulatory Supplement for Normal PB Monocyte Derived
Macrophages, 50mL (PB-MDM-003)
Cryopreservation Media:
AllCells Cryopreservation Media (CRYO-001)
Dermal Cells:
Normal Human Neonatal Dermal Fibroblast Basal Medium, 450 ml
(HN006006)
Normal Human Neonatal Dermal Fibroblast Medium Supplement, 50 ml
(HN006007)
Normal Human Neonatal Epidermal Keratinocytes Basal Medium (Undefined), 500 ml (HN007006)
Normal Human Neonatal Epidermal Keratinocytes Supplement Kit for
Undefined Medium, 5 ml (HN007007)
HUVEC:
HUVEC Basal Medium CB HUVEC, 500 ml (HUVEC-002)
HUVEC Stimulatory Supplement CB HUVEC, 10 ml (HUVEC-003)
HUVEC Stimulatory Supplement CB HUVEC, 50 ml (HUVEC-004)
MSC (adult stem cell culture):
MSC Basal Medium BM MSC, 500 ml (MSC-002)
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
174
Cell Culture & Cryopreservation Media
Catalog #
QTY/Unit
Price/Unit
DMEM-001
500 ml/bottle
$23
DMEM-002
500 ml/bottle
$23
IMDM-001
500 ml/bottle
$30
RPMI-001
500 ml/bottle
$30
CRYO-001
50 ml/bottle
$103
Product Description
Dulbeco's Modified Eagle's Medium (DMEM-001) with 4.5g/L glucose, sodium pyruvate & L-glutamine. This media contains
L-glutamine, Sodium Bicarbonate and Sodium Pyruvate.
pH 7.2 + 0.2, Osmolality 335 + 30 mOsm
Dulbeco's Modified Eagle's Medium (DMEM-002 ) with 1g/L glucose, sodium pyruvate & L-glutamine. This media contains Lglutamine, Sodium Bicarbonate and Sodium Pyruvate.
pH 7.2 + 0.2, Osmolality 335 + 30 mOsm
Iscove's Modification of DMEM (IMDM-001 ) with L-glutamine & 25mM HEPES. This media contains L-glutamine, HEPES,
Sodium Bicarbonate and Sodium Pyruvate.
pH 7.2 + 0.2, Osmolality 265 + 310 mOsm
Roswell Park Memorial Institute (RPMI-001) with L-glutamine & HEPES. This media contains L-glutamine, HEPES, and Sodium
Bicarbonate.
pH 7.2 + 0.2, Osmolality 290 + 30 mOsm
AllCells Cryopreservation Media (CRYO-001) This media contains DMSO and proprietary reagents that increase the viability of
cryopreserved cells. Store at -80 C. This media has a shelf life of 3 months from the date of manufacture when stored properly. Avoid multiple freeze thaws of this media. Cryopreservation media is shipped on dry ice. MSDS available upon request.
Stability/Storage
Cell Culture & Cryo Media
Store at 2 - 80C. Protect from light. These media has a shelf-life of 18 months from the date of manufacture when stored properly.
Avoid freezing of this media as it may cuase precipitation upon thawing.
Shipping Information
Media is shipped at 40C on cold gel pack. MSDS available upon request.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
175
Technical Notes
Technical Notes Table of Contents
•
How to Count Cells
176
•
How to Thaw Frozen Cells
177
•
How to Freeze Cell Products
178-179
•
How to Purify Bone Marrow MNC
180-181
•
How to Count Leuko Pak
182
•
How to Deplete Red Blood Cells
183
•
How to Passage & Subculture Cells:
-Fetal Liver Mesenchymal Stem Cells
184
-HUVEC
185
-Mesenchymal Stem Cells
186
-Monocyte-Derived Macrophages
187
For more information including PDF versions of the Technical Notes and our BioTube Technical Videos visit:
www.allcells.com/support/how-to/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
176
Technical Notes—How to Count Cells
When you receive an AllCells product, please count the cells
IMMEDIATELY. AllCells is not liable for any cell loss during
subsequent processing. Please note: For Leuko Paks, please refer
If your cell count is significantly lower than what AllCells claims,
to "How to Count: Leuko Pak" page 182.
1. Check the cell suspension and see if there are any cell clumps.
If so, try to separate the cells using a P1000 pipet tip. Most of
the cell clumps should be small and easily separable.
2. The optimal cell concentration is 50 to 100 cells on one large
hemacytometer square (total 200 to 400 per hemocytometer).
Please make the proper dilution or adjust the concentration
and count again.
3. Immediately report any significant discrepancies to AllCells.
Take a 10 µL sample from the vial. Mix the 10µL sample with 10µL
of trypan blue. Dilute the cells if necessary and count the number of
cells in 4 quadrants of hemacytometer (see red quadrants in figure
below right) to determine the viability.
Table 1: Common Dilution Factors
Tube Size
Cell Number/
Unit Number
Cell
Sample
0.4%Trypan
Blue Amount
PBS (1x)
Dilution
Factor
1.5 to
2mL
0.5 to1 x106
10μL
10μL
---
2
5mL
5 x106
10μL
10μL
---
2
15mL
10 x106
10μL
10μL
---
2
15mL
15 to 20 x106
10μL
10μL
80μL
10
50 mL
30 to100 x106
10μL
10μL
80μL
10
Hemacytometer Method
Hausser BrightLine Directions for Use (http://www.hausserscientific.com/hausserbrightlinedirect.htm)
Usage: cell counts
Cell Depth: 0.100mm+/- 2% (1/10mm)
Volume: 0.1 Microliter
Ruling Pattern: Improved Neubauer, 1/400 Square mm
N = # of cells counted on all 4 squares of a hemacytometer
d = dilution factor
Trypan Blue Method
Equation for Cell Count:
# of cells/vial = N / 4 x d x ____mL = _____x 104
Equation for Viability:
# of cells excluded by trypan blue / total number of cells
x 100% = ______%
Table 2: Common Dilution Factors for MNC Products with RBC
(Cord Blood and Bone Marrow Mononuclear Cell Products)
Tube Size
Cell Number/Unit
Number
Cell
Sample
*3% Acetic Acid with Methylene Blue Solution
50 mL
100-200 x106
25 μL
475 μL
50 mL
200-400 x106
25 μL
475 μL
*StemCell Technologies, Inc., Vancouver; Cat. No. 07060, Tel: 604-877-0713
Neubauer Ruling
(Diagram credit: Hausser Scientific)
To clean the counting chamber:
After counting, remove the cover glass and clean the counting
chamber with water or a mild cleaning solution (10% solution of
bleach). Dry the counting chamber with a soft cloth or wipe, or rinse
with acetone.
For a technical video of this guide visit our BioTube page at:
www.youtube.com/user/AllCells
Methylene Blue Method
Equation for Cell Count:
# of cells/vial_____ / x 20 x d x ___mL = _____ x 106
NOTE: Methylene Blue Method will not give you the cell viability. To
find cell viability use Trypan Blue Method.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Technical Notes
How to Count
In a biosafety hood, use a 5 mL pipet to mix the cell suspension
very well and to separate any cell clumps. For any cells in a 1.5 or 2
mL tube, mix cells with a P1000 pipet.
please take the following steps:
177
Technical Notes
Technical Notes—How to Thaw Frozen Cells
Shipping and Storage
For short-term storage (< 1 month), store in -80° C freezer. For
long-term storage (> 1 month), store in the “vapor phase” of a liquid
nitrogen tank.
General Medium Requirements
• IMDM, α-MEM, or RPMI-1640 containing 10% FBS
• DNase I
Thawing Cells
1. In a 37°C water bath, warm medium (IMDM, α-MEM, or RPMI1640) containing 10% FBS.
2. Clean the frozen vial with 70% alcohol before thawing. In a
biosafety hood, twist the cap a quarter-turn to relieve pressure,
and then retighten the cap.
3. In a 37°C water bath, quickly thaw the vial for approximately 2
minutes. Be careful not to submerge the entire vial in the water
bath. Do not remove the vial until a tiny ice-crystal is left.
4. Remove vial from water bath and clean the outside of the vial
with 70% alcohol.
Table 3: Typical Cell Suspension Volumes
Cell Unit
Cell Suspension Volume
< 1 x 106
1.2 ml
> 1x106
1.8 ml
9. Rinse the vial with 1ml of 37°C medium. In the 50ml conical
tube, slowly add the rinse drop by drop (5 seconds per drop) to
the cells while gently shaking the tube.
10. Next, slowly add the 37°C medium drop by drop (5 seconds
per drop) to the cells up to a total volume of 15 to 20 ml (about
10 times or more than the volume of the vial).
11. Centrifuge the cell suspension at 200xg at room temperature
for 15 minutes.
12. Using a pipet, carefully remove most of the supernatant (save
the supernatant in a second tube). Leave a few milliliters of the
supernatant behind so the cell pellet is not disturbed. Gently
resuspend the cell pellet in the remaining few milliliters of
medium.
5. In a biosafety hood, measure the cell suspension volume.
6. Count the cells using the Trypan Blue Method. Take a 10µl
sample from the vial. Mix 10µl of the sample with 10µl of trypan blue. Dilute the cells if necessary and count the number of
cells on a hemacytometer to determine the viability.
Note: DMSO is heavier than medium. Try to aspirate and discard
the supernatant soon after centrifugation.
13. While gently shaking the tube, slowly add an additional 15 to
20ml of fresh medium to the tube.
N = # of cells counted on all 4 squares of a hemacytometer
d= dilution factor
14. Centrifuge the cell suspension at 200xg (1000 RPM on Beckman Coulter™ Centrifuge) at room temperature for 15 minutes.
Equation for Cell Count: # of cells/ vial = N / 4 x d x ____ml =
_____ x 104
15. Using a pipet, carefully remove all but 2ml of supernatant.
Gently resuspend the cell pellet in the remaining 2ml of
medium and count. If the cell count is lower than expected,
centrifuge the supernatant saved in Step 11 at a higher speed,
count and combine if necessary.
16. Plate the cells in appropriate-sized dish/flask. Incubate in
a humidified incubator specified for tissue culture at 37°C
with 5% CO2. Your cells are now ready for downstream
applications.
Equation for Viability: # of cells excluded by trypan blue/ total
number of cells x 100% = ______%
7. (Optional) In order to avoid occasional clumping, when thawing
units with large cell numbers (>100x106), add DNase I to a
50ml conical tube.
a. To thaw purified cells for culture purpose, a total of 100µg
is needed.
b. To thaw mononuclear cells for cell culture purpose, a total of
300µg is needed.
For a technical video of this guide visit our BioTube page at:
www.youtube.com/user/AllCells
Note: DNase I should not be used if the cells are used for
purification of genomic DNA or if cDNA is for RNA synthesis.
8. Aseptically, using sterile techniques, transfer the cell
suspension to the 50ml conical tube, which contains DNase I.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
178
The below information is for customers who have extra fresh cell
products or have fresh cells that are not needed immediately for
processing. Please note once Cryopreservation Media is added
the process cannot be stopped and should be followed through
to completion. Freezing cells risks poorer viability and poorer cell
recovery after thaw.
How to Freeze Cell Products
(with the exception of bone marrow mononuclear cells)
Reagent and Materials Required
•
Cryopreservation Media (80% FBS, 20% DMSO)
NOTE: for better results use AllCells Cryopreservation
Media CRYO-001 (see Procedure 1. below)
•
Isocove’s Modified Dulbecco’s Medium (IMDM)
•
Ice
Equipment Required
•
Refrigerated centrifuge
•
Biosafety hood (Class II)
•
Compound microscope
•
2mL Cryogenic vials
•
Freezing rack
•
1 to 10 mL pipets
•
Conical centrifuge tubes
•
Isopropanol graded freezing containers
•
-20oC freezer
•
-80oC freezer
Procedure
1. Thaw AllCells Cryopreservation Media (CRYO-001) with proprietary blend of reagents to increase cell viability. Alternately,
prepare cryopreservation solution of 80% FBS and 20%
DMSO up to 2 days in advance and store at 4oC.
6. Based on the desired concentration of cells/vial, calculate
the amount of IMDM required to resuspend cells using the
formulas:
a. # cells/desired concentration of cells per vial = # vials
needed
b. 0.9mL/vial X # vials = # mL of Cryopreservation Media
required
Resuspend the cell pellet in this volume of cold (4oC) IMDM.
7. Mix the suspension well by pipetting up and down. Incubate
cells on ice for approximately 5 to 10 minutes.
8. In a small bowl, add ice and water to make a homogenous icewater bath.
9. Place the cell suspension tube in the ice-water bath. Add the
Cryopreservation Media to the cell suspension at a rate of 3 to
5 seconds per drop. Shake the tube constantly to ensure even
mixing.
10. Remove freezing rack from freezer and place in the hood. Mix
the cells well by pipetting and aliquot 1.8mL of cells to each
cryogenic vial.
11. Freeze cells at a controlled rate of 1oC/min by placing vials
in an alcohol-graded freezing container(s) and incubating at
-80oC for a minimum of 4 hours to a maximum of overnight.
12. Transfer vials to vapor phase of liquid-nitrogen storage.
Cells stored in the vapor phase can be stored long-term up to
several years. When commencing downstream applications on the
cells, follow the protocol on How to Thaw Cells by referring to the
Technical Note pg. 177.
2. Label cryogenic vials with the appropriate vial and donor information.
3. Place ice at the bottom of a freezing rack, place vials in the
freezing rack, store in a -20oC freezer for at least 10 minutes
until ready for use. .
4. Count the cells with preferred primary cell counting method
(see Technical Notes pg. 176)
5. Centrifuge cells at 200xg (1000 RPM on Beckman Coulter™
Centrifuge) for 5 minutes at 4oC and remove supernatant from
cell pellet.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Technical Notes
Technical Notes—How to Freeze Extra Primary Cell Products
179
Technical Notes—How to Freeze Cell Products—Bone Marrow-MNC
Technical Notes
How to Freeze Bone Marrow MNC
Reagents Required
•
Washing Buffer: PBS (Ca2+ and Mg2+ free)
with 2mM EDTA and 10% FBS
•
CryostorTM CS5
•
Fetal Bovine Serum (FBS)
•
Ice
3. Use Cryostor™ CS5. Resuspend a small amount of Cryostor
CS5 with a P1000 pipettman. Bring the cell suspension to the
required amount for freezing.
•
Refrigerated centrifuge
•
Biosafety hood (Class II)
•
2mL Cryogenic vials
•
Compound microscope
•
1 to 10 mL pipets
•
Isopropanol graded freezing containers
•
-20oC freezer
•
-80oC freezer
ALLCELLS
a. Concentration of cells <1x106 freeze at 1.2ml
b. Concentration of cells >1x106 freeze at 1.8ml
2. Centrifuge the cells in a 50ml conical tube at 200xg at 4°C for
10 minutes without brake. Remove the supernatant.
Equipment Required
Procedure
1. Determine the number of vials needed and the
concentration of freeze.
4. Aliquot the appropriate amounts of cell suspension in the
cryogenic vials for freeze.
Toll Free Phone: 1-888-535-3444
5. Place vials in isopropanol graded freezing containers and place
in a -80 °C freezer. For long-term storage (>1 month), we
recommend moving vials to the vapor phase of liquid-nitrogen
storage.
www.allcells.com
For In Vitro Research Use Only
180
BM Unit Size
Total PBS (x1) Added to BM
For Shipment
3ml
1.5ml
10ml
4 ml
25ml
15ml
50ml
30ml
100ml
60ml
The BM-MNC population is approximately 20-30% of white blood
cells.
AllCells is not responsible for the recovery number of MNC or other
purified cells. For a specific number of a certain cell type please
consider our bone marrow purified products.
Reagents Required
•
Ficoll-Paque™ PLUS or Lymphocyte Separation Media
(1.077 density)
•
Washing Buffer: PBS (Ca2+ and Mg2+ free) with
2 mM EDTA and 10% Fetal Bovine Serum
•
Fetal Bovine Serum (FBS)
•
Ice
•
0.4% Trypan Blue (to check for dead cells)
•
3% Acetic Acid Methylene Blue
Equipment Required
Procedure
1. The whole BM product is shipped to you at room temperature.
It is highly recommended to store the whole BM product at
room temperature before MNC isolation.
2. Before MNC isolation, please count the whole BM product
with methylene blue to determine the WBC number in the
sample (please refer to How to Count Fresh Cells Technical
Notes).
3. Place the cell strainer on top of a sterile 50 ml conical tube.
Wet the strainer using 1 ml PBS. Slowly, run the BM through
the strainer. Wash the strainer with 5-10 ml of PBS (without
exceeding appropriate dilution ratio); discard the strainer.
4. Bring your BM cell suspension up to a 1:2 dilution (bone marrow: PBS) in 50ml conical tubes. Dilute every 10ml of your
bone marrow product with 20ml of PBS.
5. Invert the tube several times. Carefully and slowly, under-lay
every 30ml of diluted blood with 14 ml of room temperature
Ficoll-Paque into each tube. Avoid creating air bubbles in the
Ficoll layer.
6. Centrifuge for 30 minutes at 400xg (1300 rpm for Beckman
Coulter™ Centrifuge) at 25°C in a swinging-bucket rotor
(no brake).
7. Remove the upper layer, leaving the mononuclear cell layer
undisturbed at the interphase.
8. Carefully collect the interphase cells and a small part of the
cells underneath the interphase.
•
Refrigerated centrifuge
•
Biosafety hood (Class II)
Note: Do not disturb the red pellet as this will cause granulocyte
contamination.
•
Hemacytometer
9. Transfer to a sterile 50 ml conical tube.
•
Compound microscope
•
5 to 10ml pipets
•
10 –200μl pipettors
•
10 – 200μl tips
•
50ml conical centrifuge tubes
•
40μm mesh nylon strainer (Becton Dickinson Biosciences,
REF 3523)
The following process:
•
Should be performed in a sterile biosafety hood (Class II)
to avoid bacterial contamination.
•
Is written for MNC isolation using 50ml conical centrifuge
tubes.
ALLCELLS
Toll Free Phone: 1-888-535-3444
10. Centrifuge your sample at 1700xg (3000 rpm for Beckman
Coulter™ Centrifuge) at 4°C for 10 minutes, no brake.
11. Remove the supernatant. Resuspend the cells with 5 ml of cold
(4°C) Washing Buffer, using a 5ml pipet.
12. Fill the tube up to 50ml with cold Washing Buffer.
13. Count the cells using the Methylene Blue Method (See "How to
Count Fresh Cells").
14. Centrifuge at 200xg (1000 rpm for Beckman Coulter™ Centrifuge) at 4°C for 10 minutes, no brake.
15. Resuspend the cell pellet with proper culture media and count
the cells. Adjust the MNC concentration with proper culture
media and proceed to your experiment.
www.allcells.com
For In Vitro Research Use Only
Technical Notes
TechnicalNotes
Notes
Technical
Technical Notes—How to Purify Bone Marrow MNC
181
Technical Notes—How to Purify Bone Marrow MNC (continued)
Technical Notes
Frequently Asked Questions
Q. Can bone marrow cells be used in colony formation assays?
A. Yes, our purified bone marrow CD34+ cells have been used in
colony formation assays, and they perform extremely well.
Q. After ficolling, my BM-MNC have many red blood cells (RBC). Is
this normal? If so, how do I get rid of them?
A. It is extremely difficult to collect only MNC during ficolling.
The amount of RBC you harvest depends on donor variation and
technique. You can use Ammonium Chloride for RBC lysis.
Note: Excessive exposure to ammonium chloride can potentially
damage leukocytes.
Q. How do I get rid of clumps in my mononuclear cells?
A. You can use a 40 μm cell strainer to strain the clumps out.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
182
Technical Notes—How to Count: Leuko Pak
Reagent and Materials Required
tube with 475 µl Methylene Blue.
7. Add 80µl of DPBS into a mixing well (rounded non-sterile 96
well plate). Invert the 1.5 ml tube several times to mix the blood
and Methylene Blue thoroughly.
•
Sterile Room Temperature Washing Buffer: 500mL DPBS
with 2mM EDTA
•
Cold Washing Buffer: 500mL DPBS+10mL Fetal Calf
Serum (FCS) or Fetal Bovine Serum (FBS) + 2mM EDTA
•
Cold Shipping Buffer: 500mL DPBS+8mL Bovine Serum
Albumin (BSA; 0.5%)
•
Ammonium Chloride Solution (0.8% NH4Cl with 0.1mM
EDTA). Use approximately 10 to 15ml of ammonium
chloride per 2-5x108 MNC per tube.
a. Methylene Blue Method Equation for Cell Count:
# of cells/vial_____ / 20 x d x ___mL = _____ x 106
•
50mL Conical Tubes
•
5mL and 25mL pipets
•
Latex gloves
b. To assess viability, use Trypan Blue Method. Mix equal volumes of cell to trypan blue (10µl:10µl) and dilute appropriately
where necessary.
Equipment Required
•
Class II Biological Safety Cabinet
•
Beckman Centrifuge
•
Pipettors
•
Stainless Steel Surgical Scissors
•
Stainless Steel Clamps
Safety Precautions
•
•
•
8. Take a 20µl sample from the 1.5 ml tube and mix with 80µl
DPBS. Load the hemacytometer with 20 µl of diluted sample.
9. Count the total number of mononucelar cells using Methylene
Blue Method.
Equation for Viability:
# of cells excluded by trypan blue/ total number of cells x
100% = ______%
To Process PB-MNC from Leuko Pak
1. Count the cells from Leuko pak as detailed above.
2. Refer to "How to Deplete RBC," page 183.
Protective gloves should be worn at all times.
Use a safety pipetting device for all pipetting.
NEVER PIPET BY MOUTH
Procedure
1. Upon receipt of the apheresis blood, verify that the product
label is correct and donor information sheet is provided.
2. Remove the blood bag from biohazard bag. Place the
apheresis bag in a Biological Safety Hood and examine the
bag for leaks or clumps.
3. Mix the blood bag well and locate the blood transfer line.
4. Disinfect the blood transfer line with an alcohol swab and
cut using sterile scissors.
5. Wipe the cut blood transfer line with an alcohol swab.
Wait 1 minute until any remaining alcohol has evaporated.
Carefully place 5ml of the blood in a sterile 50ml conical
centrifuge tube and clamp the transfer pack to prevent any
spills.
6. Take 25µl sample of blood and place into 1.5 ml centrifuge
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Technical Notes
Blood is obtained from normal healthy volunteers with collection
of maximum three-blood volume on COBE SpectraTM apheresis
machine.
183
Technical Notes—How to Deplete Red Blood Cells
Technical Notes
This protocol is designed for human peripheral blood, bone marrow and cord blood for red blood cells (RBC) preparations. This protocol
outlines the procedure for depletion of RBC from whole blood and from mononuclear cells.
Procedure For RBC Lysis from Mononuclear Cells
Materials and Reagents
•
Dulbecco’s Phosphate Buffered Saline (DPBS) with 2mM
EDTA
•
WB (washing buffer, 2% Fetal Bovine Serum (FBS), 2mM
EDTA in DPBS)
•
Ammonium Chloride (StemCell Technologies Cat# 07850)
Refrigerated centrifuge
•
Ice in a bucket
•
Pipettor
•
Biosafety hood
2. Discard the supernatant and resuspend cells with 25 ml of
WB.
3. Centrifuge the tube at 200xg (1000 rpm) for 10 minutes at
room temperature.
Equipment
•
1. Centrifuge the cells after collecting the lymphocyte layer from
the separation medium.
4. Discard the supernatant and add the Ammonium Chloride at
4:1 ratio. For example, 4ml of Ammonium Chloride to 1ml of
blood.
5. Mix the cells and Ammonium Chloride and place on ice for 10
minutes to allow lysis of the RBC.
Safety Precautions
•
Protective gloves should be worn at all times
•
Use a safe pipetting device for all pipetting
•
NEVER PIPET BY MOUTH
6. Fill the tube with WB up to 25ml and centrifuge the tube at
200xg (1000rpm) for 10 minutes at 4oC.
7. Discard the supernatant and wash the cells twice with WB.
The cells are ready for use.
Procedure For Lysis of RBC From Whole Blood
1. Transfer 25ml of blood into a 50ml conical tube and centrifuge
at 200xg (or 1000 rpm on Beckman Coulter™ Centrifuge) in a
swinging bucket rotor for 10 minutes at room temperature.
2. Discard the supernatant and resuspend the blood with 25ml of
DPBS (containing 5mM EDTA) and centrifuge at 200xg (1000
rpm) for 10 minutes at room temperature.
3. Discard the supernatant and add the Ammonium Chloride at
9:1 ratio. For example, 9ml of Ammonium Chloride to 1ml of
blood. You should have 45ml of Ammonium Chloride to 5ml of
blood.
4. Mix the cell and Ammonium Chloride and place on ice for 10
minutes to allow lysis of the RBC.
5. Centrifuge the tube at 500xg (1500rpm) for 10 minutes in
4oC.
6. Discard the supernatant and wash the cells twice with WB. The
cells are ready for use.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
184
Thawing and Culturing Cells
1. Calculate the number of flasks needed. The recommended
seeding density for AllCells FL-MSC is 5,000 to 10,000 cells/
cm2. For example, one vial of AllCells MSC (7.5x105/vial) is
sufficient for one to two T75 flasks or three to six T25 flasks.
2. Prepare the culture media:
a. Add 2mM concentration of L-glutamine and the entire
contents of 50ml AllCells Fetal MSC Stimulatory Supplement
(Cat. No. FL-003) to 450ml of AllCells Basal Medium (Cat. No.
FL-002).
b. Add 2-3 ml/5 cm2 of the prepared Fetal MSC Media into the
T75 flasks.
c. In a 37°C, 5% CO2 humidified incubator, place the media in
flasks to equilibrate in the incubator for at least 30 minutes.
3. Wipe the frozen vial with 70% alcohol before thawing. In a
biosafety hood, briefly twist the cap a quarter-turn to relieve
pressure, and then retighten the cap.
4. In a 37°C water bath, quickly thaw the vial. Be careful not to
submerge the entire vial in the water bath. Do not remove the
vial until a tiny ice-crystal is left. Wipe the outside of the vial
with 70% alcohol.
5. Count the cells using Trypan Blue Method. In a biosafety hood,
take a 10ul sample from the vial. Mix the 10ul sample with
10µl of trypan blue. Dilute the cells if necessary and count the
number of cells on a hemacytometer to determine the viability.
N = # of cells counted on all 4 squares of a hemacytometer
d= dilution factor
Trypan Blue Method Equation for Cell Count:
# of cells/ vial =N/4 x d x_____ ml = _____104
Equation for Viability:
# of cells excluded by trypan blue/ total #of cells x 100% =
______%
**This is a very important step to determine if the cell number
viability matches what AllCells claims.
Maintaining Cell Cultures
1. Change the growth medium the day after seeding and every
other day thereafter.
2. In a sterile container, warm an appropriate amount of medium
to 37°C.
ALLCELLS
Toll Free Phone: 1-888-535-3444
3. Remove the medium from the culture flasks and replace with
the warmed, fresh medium; return the flask to the incubator.
Note: Avoid repeated warming and cooling of the medium. If the
entire contents are not needed for a single procedure, transfer only
the required volume to a sterile secondary container.
Subculturing Cells
1. Subculture the cells when they are 70 to 80% confluent
(about 10 days). Many mitotic figures should be visible
throughout the flask.
2. For each 25cm2 of cells to be subcultured: (1) Prepare 2 ml
of Trypsin/EDTA, 5 ml of HEPES Buffered Saline Solution
(HEPES-BSS), 4 ml of Trypsin Neutralizing Solution (TNS
such as 10% FBS-DPBS). Allow the aforementioned items to
come to room temperature; (2) From one culture flask, remove
the medium and rinse the flask with 5 ml of room temperature
HEPES-BSS. (This is an IMPORTANT step. The culture
medium contains complex proteins that neutralize the effect of
trypsin.) Remove the HEPES-BSS from the flask; (3) Repeat
step 2 for each culture flask.
3. Add 2 ml of Trypsin/EDTA solution to each T25 flask and
incubate at 37°C. Allow the trypsinization to continue until approximately 90% of the cells have lifted up from the T25 flask.
This entire process takes about 2 to 6 minutes.
4. Hit the flask against the palm of your hand to release the
majority of cells from the culture surface. If only a few cells detach, wait 30 seconds and repeat this motion again. If cells still
do not detach, wait and repeat every 30 seconds thereafter.
5. After cells have detached, neutralize the trypsin in the flask
with 4 ml of room temperature Trypsin Neutralizing Solution.
6. Quickly transfer the detached cells to a sterile 15ml centrifuge
tube and rinse the flask with a final 2 ml of HEPES-BSS to
collect residual cells.
7. Add this rinse to the centrifuge tube.
8. Under the microscope, examine the harvested flask to make
sure the harvest was successful. There should be less than 5%
of the cells remaining.
9. Centrifuge the harvested cells at 200xg (1000rpm on Beckman Coulter™ Centrifuge) for 5 minutes to pellet the cells.
10. Dilute the cells in 4 to 5 ml of growth medium and count the
cells with a hemacytometer.
11. Culture the cells. (Refer to Thawing and Culturing Cells)
www.allcells.com
For In Vitro Research Use Only
Technical Notes
Technical Notes—How to Passage and Subculture—
Fetal Liver Mesenchymal Stem Cells
185
Technical Notes
Technical Notes—How to Passage and Subculture—HUVEC
General Media Requirements
HUVEC can achieve 15 population doublings with AllCells HUVEC
Basal Medium (HUVEC-002) and AllCells HUVEC Basal Medium
Supplement (HUVEC-003).
Thawing and Culturing Cells
1. Calculate the number of flasks needed. The recommended
seeding density for AllCells HUVEC is 2,500 to 5,000 cells/
cm2. For example, one vial of AllCells HUVEC (5x105/vial) is
sufficient for four to eight T25 flasks.
2. Prepare the flasks and the culture media. Add 2ml of 0.5%
gelatin to each T25 flask or 5ml 0.5% of gelatin to each T75
flask. At 37°C, incubate for >2 hours or overnight. After the
incubation, discard the gelatin and wash the flask once with
10ml DPBS. To the 90ml of AllCells HUVEC Basal Media
(HUVEC-002), add the entire contents of 10ml AllCells HUVEC Basal Medium Supplement (HUVEC-003). Add 1 ml/5
cm2 of the mixed media to the gelatin-coated flasks. In a 37°C,
5% CO2 humidified incubator, allow the flasks to equilibrate for
at least 30 minutes.
3. Wipe the frozen vial with 70% alcohol before thawing. In a
biosafety hood, briefly twist the cap a quarter-turn to relieve
pressure, then retighten the cap.
4. In a 37°C water bath, quickly thaw the vial. Be careful not to
submerge the entire vial in the water bath. Do not remove the
vial until a tiny ice-crystal is left. Wipe the outside of the vial
with 70% alcohol.
5. Resuspend and dispense cells from the vial into the culture
flasks set up earlier. Gently rock the culture flasks to evenly
distribute the cells and return to the incubator.
Note: It is not necessary to wash the cells. Washing the cells is
more damaging than the effects of DMSO residue in the culture.
Maintaining Cell Culture
6. Change the growth medium the day after seeding and every
other day thereafter.
7. In a sterile container, warm an appropriate amount of medium
to 37°C. Remove the medium and replace it with the warmed,
fresh medium. Return the flask to the incubator.
9. For each 25cm2 of cells to be subcultured:
a. Prepare 2 ml of Trypsin/EDTA, 5 ml of HEPES Buffered
Saline Solution (HEPES-BSS), 4 ml of Trypsin Neutralizing
Solution (TNS such as 10% FBS-DPBS). Allow the
aforementioned items to come to room temperature.
b. From one culture flask, remove the medium and rinse the
flask with 5 ml of room temperature HEPES-BSS. (This is
an IMPORTANT step. The culture medium contains complex
proteins that neutralize the effect of trypsin.) Remove the
HEPES-BSS solution from the flask;
c. Repeat Step b for each culture flask.
10. Add 2 ml of Trypsin/EDTA solution to each T25 flask. Incubate
cells with trypsin at 37°C for 2-6 minutes until approximately
90% of the cells have lifted. Hit the flask against the palm of
your hand to release the majority of cells from the culture surface. If only a few cells detach, wait 30 seconds and repeat this
motion again. If cells still do not detach, wait and repeat every
30 seconds thereafter.
11. After cells have detached, neutralize the trypsin in the flask
with 4 ml of room temperature Trypsin Neutralizing Solution.
12. Quickly transfer the detached cells to a sterile 15ml centrifuge
tube and rinse the flask with 2 ml of HEPES-BSS to collect
residual cells. Add this rinse to the centrifuge tube.
13. Under the microscope, examine the harvested flask to make
sure the harvest was successful. There should be less than 5%
of the cells remaining.
14. Centrifuge the harvested cells at 200xg (1000rpm on Beckman Coulter™ Centrifuge) for 5 minutes to
pellet the cells.
15. Dilute the cells in 4-5 ml of complete HUVEC Medium and
count the cells with a hemacytometer.
16. Culture the cells (Refer to the Thawing and Culturing Cells
section).
10. Warm an appropriate amount of medium to 37°C.
Note: Avoid repeated warming and cooling of the medium. If the
entire contents are not needed for a single procedure, transfer only
the required volume to a sterile secondary container.
Subculturing Cells
8. Subculture the cells when they are 70 to 80% confluent
(about seven days). Many mitotic figures should be visible
throughout the flask.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
186
Thawing and Culturing Cells
1. Calculate the number of flasks needed. The recommended
seeding density for AllCells BM Mesenchymal Stem Cells
(MSC) is 5,000 to 10,000 cells/cm2. For example, one vial of
AllCells MSC (7.5x105/vial) would be sufficient for 1 to 2 T75
flasks or 3 to 6 T25 flasks.
2. Prepare the culture media:
a. Add 2mM concentration of L-glutamine and the entire
contents of 50ml AllCells Stimulatory supplement (Cat.
No.MSC-003)
to 450ml of AllCells Basal Medium (Cat. No. MSC-002).
b. Add 2 to 3 ml/5 cm2 of the mixed media into the T75 or
T25 flasks.
c. In a 37°C, 5% CO2 humidified incubator allow the flasks to
equilibrate in for at least 30 minutes.
3. Wipe the frozen vial with 70% alcohol before thawing. In a
biosafety hood, briefly twist the cap a quarter-turn to relieve
pressure, then retighten the cap.
4. In a 37°C water bath, quickly thaw the vial. Be careful not to
submerge the entire vial in the water bath. Do not remove the
vial until a tiny ice-crystal is left. Wipe the outside of the vial
with 70% alcohol.
5. In a biosafety hood, take a 10ul sample from the vial. Mix
the 10µl sample with 10µl of trypan blue. Dilute the cells if
necessary and count the number of cells on a hemacytometer
to determine the viability.
N = # of cells counted on all 4 squares of a hemacytometer
d= dilution factor
Equation for Cell Count:
# of cells/ vial = N/4 x d x____ml = _____104
Equation for Viability:
# of cells excluded by trypan blue/ # total number of cells x 100%
= ______%
Note: Avoid repeated warming and cooling of the medium. If the
entire contents are not needed for a single procedure, transfer only
the required volume to a sterile secondary container.
Subculturing Cells
1. Subculture the cells when they are 70 to 80% confluent
(about 10 days). Many mitotic figures should be visible
throughout the flask.
2. For each 25cm2 of cells to be subcultured:
a. Prepare 2 ml of Trypsin/EDTA, 5 ml of HEPES Buffered
Saline Solution (HEPES-BSS), 4 ml of Trypsin Neutralizing
Solution (TNS such as 10% FBS-DPBS). Allow the
aforementioned items to come to room temperature.
b. From one culture flask, remove the medium and rinse the
flask with 5 ml of room temperature HEPES-BSS. (This is
an IMPORTANT step. The culture medium contains complex
proteins that neutralize the effect of trypsin.) Remove the
HEPES-BSS from the flask.
c. Repeat Step b for each culture flask.
3. Add 2 ml of Trypsin/EDTA solution to each T25 flask. Allow
the trypsinization to continue until approximately 90% of the
cells have lifted up from the T25 flask. This entire process
takes about two to six minutes. Hit the flask against the palm
of your hand to release the majority of cells from the culture
surface. If only a few cells detach, wait 30 seconds and repeat
this motion again. If cells still do not detach, wait and repeat
every 30 seconds thereafter.
4. After cells have detached, neutralize the trypsin in the flask
with 4 ml of room temperature Trypsin Neutralizing Solution.
5. Quickly transfer the detached cells to a sterile 15ml centrifuge
tube and rinse the flask with a final 2 ml of HEPES-BSS to
collect residual cells. Add this rinse to the centrifuge tube.
**This is a very important step to determine if the cell viability number matches what AllCells claims.
Maintaining Cell Culture
1. Change the growth medium the day after seeding and every
other day thereafter.
2. In a sterile container, warm an appropriate amount of Complete
Medium to 37°C.
3. Remove the medium and replace with the warmed, fresh
medium; return the flask to the incubator.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Technical Notes
Technical Notes—How to Passage and Subculture—
Mesenchymal Stem Cells
187
Technical Notes
Technical Notes—How to Passage and Subculture—
Monocyte-Derived Macrophages
General Media Requirements
Monocyte Derived Macrophages (MDM) will mature if cultured with
Macrophage Basal Medium (Cat. No. PB-MDM-002) in conjunction
with Macrophage Stimulatory Supplement (Cat. No. PB-MDM-003).
Types of Culture Vessels
• 96 well plate
• 35mm tissue culture dish
• 100mm tissue culture dish
• T25 flask
• T75 flask
N = # of cells counted on all 4 squares of a hemacytometer
d= dilution factor
Equation for Cell Count:
# of cells/ vial = N/4 x d x____ml = _____104
Equation for Viability:
# of cells excluded by trypan blue/ # total number of cells x
100% = ______%
**This is a very important step to determine if the cell viability
number matches what AllCells claims.
8. Initiate the culture. Resuspend and dispense 10,000 cells/cm2
from the vial into the culture vessel.
Thawing and Culturing Cells
1. Calculate the number of culture vessels needed. The
recommended seeding density for AllCells MDM is 10x104
cells/cm2. For example, one vial of AllCells MDM (1.5x106/vial)
would be sufficient for six T25 flask or two 100mm dish.
9. Add the desired volume of prepared culture medium to the
vessel.
2. Prepare the Complete Culture Medium: Add 2mM concentration of L-glutamine, 1% of the antibiotic-antimycotic stock
solution and the entire contents of 50ml AllCells Stimulatory
supplement (Cat. No. PB-MDM-003) to 450ml of AllCells
Basal Medium (Cat. No. PB-MDM-002).
Note: It is not necessary to wash the cells because this action is
more damaging than the effects of DMSO residue in the culture.
3. Wipe the frozen vial with 70% alcohol before thawing.
4. In a biosafety hood, briefly twist the cap a quarter-turn to
relieve pressure, then retighten the cap.
5. In a 37°C water bath, quickly thaw the vial. Be careful not to
submerge the entire vial. Do not remove the vial until a tiny
ice-crystal is left.
6. Wipe the outside of the vial with 70% alcohol.
7. Count cells by taking 10μl from the vial to mix with 10μl of
trypan blue solution. Count cells with a hemacytometer .
ALLCELLS
Toll Free Phone: 1-888-535-3444
10. Gently rock the culture vessel to evenly distribute the cells
and place in 5% CO2 incubator.
Maintaining Cell Culture
1. Change the growth medium the day after seeding and
every other day thereafter.
2. In a sterile container, warm an appropriate amount of complete
culture medium to 37°C. Remove the medium and replace it
with the warmed, fresh medium
3. Return the flask to the incubator.
Note: Avoid repeated warming and cooling of the medium. If
the entire contents are not needed for a single procedure, transfer
only the required volume to a sterile secondary container.
www.allcells.com
For In Vitro Research Use Only
188
A
Acute Lymphoid Leukemia (ALL) 114-119
Bone Marrow 117-119
CD19+ B Cells 119
Mononuclear Cells 118
Plasma 117
Peripheral Blood 114-116
CD19+ B Cells 116
Mononuclear Cells 115
Plasma 114
Acute Myeloid Leukemia (AML) 120-124
Bone Marrow 122-124
CD34+ Cells 124
Mononuclear Cells 123
Plasma 122
Peripheral Blood 120-121
Mononuclear Cells 121
Plasma 120
Adipose-derived Stem Cells 103-104
Amniotic/Placental Cells 103, 105
Animal Primary Cells 156-172
Apheresis Collection (Leuko Pak) 6, 16-18, 33, 36, 39, 61-62,
182
Autoimmune Diseased Cells 143-150
B
B Cells 41-43, 89, 116, 119, 128, 131
Basophils 53
Bioservices 19-27, 30-32
Bone Marrow (Human, Healthy) 16-18, 69-78
Aspirates 16-18, 70
CD34-Depleted Mononuclear Cells 72
Mononuclear Cells 71-72
Myeloid Cells 75-76
CD33+ Cells 76
CD36+ Early Erythroid Progenitor Cells 75
Progenitor Cells-CD133+ Cells 74
Stem/Progenitor Cells - CD34+ Cells 73
Mesenchymal Cells 77-78
CD105+ Endothelial Cells 77
Mesenchymal Stem Cells 78
Whole 70
C
CD133+ Cells 66, 74, 85, 100
CD34+ Cells 40, 65, 73, 84, 99, 124, 136, 158
ALLCELLS
Toll Free Phone: 1-888-535-3444
Cell-based Assays 20-22
Cell Culture Media 173-174
Cell Formats 11-12
Cell Separation Methods 11
Chronic Lymphoid Leukemia (CLL) 126-131
Bone Marrow 129-131
CD19+/CD5+ B Cells 131
Mononuclear Cells 130
Plasma 129
Peripheral Blood 126-128
CD19+/CD5+ B Cells 128
Mononuclear Cells 127
Plasma 126
Chronic Myeloid Leukemia (CML) 132-136
Bone Marrow 134-136
CD34+ Cells 136
Mononuclear Cells 133
Plasma 132
Peripheral Blood 132-133
Mononuclear Cells 133
Plasma 132
Compound treatment 24
Cord Blood 79-95
CD34-Depleted Mononuclear Cells 82
CD133-Depleted Mononuclear Cells 83
Human Umbilical Vascular Endothelial Cells (HUVEC) 95
Lymphoid Cells 89-94
CD3+ Pan T Cells 90
CD4+/CD45RA+ Naïve T Cells 93
CD4+ Helper T Cells 91
CD8+ Cytotoxic T Cells 92
CD19+ B Cells 89
CD56+ NK Cells 94
Mononuclear Cells (MNC) 81
Myeloid Cells
CD14+ Monocytes 88
CD36+ Early Erythroid Progenitor Cells 86
Neutrophils 87
Plasma 80
Stem/Progenitor Cells
CD34+ Cells 84
CD133+ Cells 85
Crohn's Disease Cells 153
Cryopreservation Media 33, 173-174, 178
Custom Products/Projects 9, 17-18, 30-32, 109-110
Culture Media 173-174
Cytotoxic T Cells 49-50, 90, 92
www.allcells.com
For In Vitro Research Use Only
Index: Alphabetical
Index - Alphabetical
189
Index: Alphabetical
Alphabetical Index
D
I
Dental Pulp Stem Cells 106
Delivery Information 6-8
Dendritic Cells 22, 36, 51, 59
Differentiated Cells 22, 106
Diffuse Large B Cell Lymphoma (DLBCL) 109-110
Diseased Cells 107-154
DNA Analysis 23
Immunoassays 21
Immunophenotyping 23, 27
Immunoprecipitation 25
In vitro proliferation/inhibition assays 24
Inflammatory Bowel Diseased Cells 152-154
ISHAGE 23
L
E
Early Erythroid Progenitor Cells (CD36+) 62, 73-75, 86, 98, 101
ELISA, ELISpot 21
Endothelial Cells 77, 95
Eosinophils 39, 54, 113
Erythrocytes 22, 36, 56
F
M
Fetal Liver 96-102
Early Erythroid Progenitor Cells (CD36+) 101
Mononuclear Cells 98
Stem/Progenitor Cells
CD34+ Cells 99
CD133+ Cells 100
Mesenchymal Stem Cells 102
Unprocessed Fetal Liver 97
Flow Cytometry 23, 24
Fluorescence-activated Cell Sorting (FACS) 23
Follicular Lymphoma (FL) 109-110
G
G-CSF Mobilized Cells 17, 61-68
General Product Information 10-12
Genotyping Services 26
Granulocytes 22, 54-55, 71, 76, 81, 87
H
Helper T Cells 45, 47, 91
Hematopoietic Assays 22
HLA-Typing 27
How To Count Fresh Cells 176
How To Freeze Cells 178-179
How To Lyse RBC 183
How To Passage and Subculture Cells 184-187
How to Purify Bone Marrow MNC 180-181
How to Thaw Frozen Cells 177
Human Umbilical Vein Endothelial Cells (HUVEC) 95
ALLCELLS
Leukemia Cells 112-136
Leuko Pak (Apheresis Collection) 6, 16-18, 33, 36, 39, 61-62, 182
Leukocytes 23, 36, 54-55, 87
Lupus (SLE) Diseased Cells 144
Toll Free Phone: 1-888-535-3444
Macrophages 22, 60, 96-97, 173-174, 187
Mantle Cell Lymphoma (MCL) 109-110
Media 173-174
Megakaryocytes 22, 40
Memory T, B Cells 43, 48
Mesenchymal Stem Cells (MSC) 22, 69, 78, 102, 160, 165, 173174, 184, 186
Mobilized Peripheral Blood 61-68
CD34-Depleted Mononuclear Cells 64
Leuko Pak 61-62
Lymphoid Cells
CD56+ NK Cells 68
Mononuclear Cells 63-64
Myeloid Cells
CD14+ Monocytes 67
Stem/Progenitor Cells
CD34+ Cells 65
CD133+ Cells 66
Monocytes 58-60, 67, 88
Mouse Cells 159-163
Astrocytes 161
Bone Marrow MNC 159
Bone Marrow MSC 160
Splenocytes 162-163
Mozobil 17, 61-62
Multiple Myeloma (MM) 137-142
Bone Marrow 140-142
CD138+ Plasma Cells 141
Mononuclear Cells 142
Plasma 140
Peripheral Blood 138-139
Mononuclear Cells 139
Plasma 138
www.allcells.com
For In Vitro Research Use Only
190
Alphabetical Index
Multiple Sclerosis (MS) Diseased Cells 145
Mutation Analysis 26
Myelodysplastic Syndrome Diseased Cells 125
Q
N
R
Natural Killer Cells 52, 68, 94
Neutrophils 37, 55, 87
Non-Hematopoietic Stem Cells 103-106
Non-Hodgkin’s Lymphoma 109-110
Non-Human Primate Cells 158
Normal Peripheral Blood 35-60
Leuko Pak 35-36
Lymphoid Cells
BDCA4+ Lymphoid/Plasmacytoid Dendritic Cells 51
CD4+/CD25+ Regulatory T Cells 46
CD4+/CD45RA+/CD25- Naïve T Cells 47
CD4+/CD45RO+ Memory T Cells 48
CD4+ Helper T Cells 45
CD8+/CD45RA+ Naive Cytotoxic T Cells 50
CD8+ Cytotoxic T Cells 49
CD19+ B Cells 41
CD19+/IgD+ Naive B Cells 42
CD19+/CD27+ Memory B Cells 43
CD56+ NK Cells 52
Pan T Cells 44
Mononuclear Cells 39
Myeloid Cells
Basophils 53
CD14+ Monocytes 58
Eosinophils 54
Mature Erythrocytes (RBC) 56
Monocyte Derived Immature Myeloid Dendritic Cells 59
Monocyte Derived Macrophages 60
Neutrophils 55
Platelets 57
Plasma 38
Stem/Progenitor CD34+ 40
Whole Blood 37
Rat Cells 164-171
Astrocytes 167
Bone Marrow —CD90 Cells 166
Bone Marrow —Mesenchymal Stem Cells 165
Bone Marrow—Mononuclear Cells 164
Meningeal 168
Pancreatic Islets 169
Splenocytes 170-171
Red Blood Cells 56
Rheumatoid Arthritis Diseased Cells 148
O
S
Sickle Cell Anemia Diseased Cells 151
T
T Cells 44-50, 90-93
Technical Notes 176-187
Technical Support 9, 19-27, 30-32
Terms & Conditions 13-14
Tissue Collection Facility 15-18
Tissue Culture Services 24
Toxicology Studies 24
Type I Diabetes Diseased Cells 149
Type II Diabetes Diseased Cells 150
U
Ulcerative Colitis Diseased Cells 154
Using the catalog 5
W
Waldenström’s Macroglobulinemia (WM) 109-110
Website Information 9
Western Blot 25
Ordering Information 6-8, 9
Osteoarthritis Diseased Cells 146
P
Pancreatic Islets 105, 169
Pig Cells 172
Placental/Amniotic Cells 103, 105
Plasmacytoid Dendritic Cells 51
Platelets 57
Protein Analysis 25
Psoriasis (PSO) Diseased Cells 147
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Index: Alphabetical
Quick Reference Guide 199-200
191
Index - BY catalog # - ABM-CB
Index: By Catalog #
A
ABM001
ABM001-1 ABM002
ABM003
ABM004
ABM005
ABM006
ABM007
ABM007F
ABM008F
ABM009
ABM009F
ABM010
ABM010F
ABM011
ABM011F
ABM012
ABM013
ABM014
ABM015F
ABM017F
ABM018F
ABM019F
ABM020F
ABM021F
ABM022F
ABM023F
ABM024
ABM025
ABM026F
ABM027
ABM027F
ABM028
ABM028F
ABM032-2
ABM032F-2
ALL-B006F
ALL-B007F
ALL-B012F
ALL-B013F
ALL-B016F
ALL-BM001F
ALL-BM003F
ALL-BM011F
ALL-BM012F
ALLCELLS
70
70
70
70
70
71
71
71
71
71
73
71
73
71
73
71
73
73
73
73
73
73
73
75
73
73
72
71
74
74
74
74
77
77
76
76
116
116
119
119
119
118
118
117
117
Toll Free Phone: 1-888-535-3444
ALL-PB001F
ALL-PB003F
ALL-PB011F
ALL-PB012F
AML-BM001F
AML-BM003F
AML-BM006F
AML-BM007F
AML-BM009F
AML-BM010F
AML-BM012F
AML-BM015F
AML-PB001F
AML-PB003F
AML-PB011F
AML-PB012F
ASC001F
115
115
114
114
123
123
124
124
124
124
122
122
121
121
120
120
104
C
CB001
CB002
CB002F
CB003F
CB004F
CB005
CB005F
CB005-S
CB005F-S
CB006
CB007F
CB007F-S
CB008F
CB008F-S
CB009F
CB010M-F
CB011
CB011F
CB011F-M
CB012
CB012-S
CB013F
CB013F-S
CB014F
CB019F
CB020-1
CB020-1F
www.allcells.com
81
81
81
81
81
84
84
84
84
84
84
84
84
84
84
86
82
82
82
85
85
85
85
83
89
90
90
For In Vitro Research Use Only
192
Catalog # Index - CB-CLL
ALLCELLS
118
118
115
115
123
123
121
121
128
128
Toll Free Phone: 1-888-535-3444
cDNA-CLL-B009
cDNA-CLL-B010
cDNA-CLL-BM001
cDNA-CLL-BM002
cDNA-CLL-PB001
cDNA-CLL-PB002
cDNA-FL001 99
cDNA-FL002 100
cDNA-FL003 98
cDNA-FL004 97
cDNA-MBM-001
cDNA-MMSC-001
cDNA-mPB001
cDNA-mPB004
cDNA-mPB005
cDNA-mPB009
cDNA-mPB010
cDNA-MSPL-001
cDNA-MSPL-011
cDNA-MSPL-012
cDNA-MSPL-013
cDNA-PB001 39
cDNA-PB002 44
cDNA-PB003 41
cDNA-PB004 58
cDNA-PB005 52
cDNA-PB007 45
cDNA-PB008 49
cDNA-PB011 55
cDNA-PB013 59
cDNA-PB014 46
cDNA-PB015 57
cDNA-PB016 47
cDNA-PB017 50
cDNA-PB018 48
cDNA-PB020 42
cDNA-PB021 43
cDNA-PB-MDM-001
cDNA-RACR-001
cDNA-RAHP-001
cDNA-RBM-001
cDNA-RBM-005
cDNA-RCTX-001
cDNA-RSPL-001
cDNA-RSPL-011
cDNA-RSPL-012
cDNA-RSPL-013
cDNA-SD-ILT 169
cDNA-WB001 37
CLL-B013F
128
CLL-B014F
128
CLL-B019F
128
CLL-B020F
128
www.allcells.com
131
131
130
130
127
127
159
160
63
67
68
65
66
162
163
163
163
60
167
167
164
165
167
170
171
171
171
For In Vitro Research Use Only
Index: By Catalog #
CB020-2
91
CB020-2F
91
CB020-3
92
CB020-3F
92
CB020-4-1
93
CB020-4-1F 93
CB020-4-2
93
CB020-4-2F 93
CB020-4-3
93
CB020-4-3F 93
CB020F
89
CB021
89
CB021F
89
CB022
88
CB022F
88
CB023
94
CB023F
94
CB026
87
CB027
80
CB027F
80
CB028
80
CB028F
80
CB029
80
CB029F
80
CB030
80
CB030F
80
CB031
80
CB031F
80
cDNA-ALL-BM001
cDNA-ALL-BM002
cDNA-ALL-PB001
cDNA-ALL-PB002
cDNA-AML-BM001
cDNA-AML-BM002
cDNA-AML-PB001
cDNA-AML-PB002
cDNA-BM001 70
cDNA-BM002 71
cDNA-BM006 76
cDNA-BM012 78
cDNA-BM013 75
cDNA-CB001 81
cDNA-CB002 90
cDNA-CB003 89
cDNA-CB004 88
cDNA-CB006 91
cDNA-CB007 93
cDNA-CB008 92
cDNA-CB014 87
cDNA-CB015 82
cDNA-CB016 83
cDNA-CLL-B005
cDNA-CLL-B006
193
Catalog # Index - CLL-MM
Index: By Catalog #
CLL-B037F
131
CLL-B038F
131
CLL-B043F
131
CLL-B044F
131
CLL-BM001F 130
CLL-BM003F 130
CLL-BM011F 129
CLL-BM012F 129
CLL-PB001F 127
CLL-PB002F 127
CLL-PB003F 127
CLL-PB007F 127
CLL-PB011F 126
CLL-PB012F 126
CML-BM001F 135
CML-BM003F 135
CML-BM008-1F
136
CML-BM008-2F
136
CML-BM009-1F
136
CML-BM009-2F
136
CML-BM011F 134
CML-BM012F 134
CML-PB001F 133
CML-PB003F 133
CML-PB011F 132
CML-PB012F 132
CRO-FPB05 153
CRO-FPB10 153
CRO-FPB30 153
CRO-FPB011F 153
CRO-PB001F 153
CRYO-001
174
D
ALLCELLS
FL-001F
102
FL-002
102
FL-003
102
FL004-T1-F
98
FL005-T1-F
98
FL006-T1-F
98
FL007-T1-F
98
FL008F-T2-F 98
FL009F-T2-F 98
FL010F-T2-F 98
FL011F-T2-F 98
FL-CD133-001F
FL-CD133-006F
FL-CD133-008F
FL-CD34-001F
FL-CD34-002F
FL-CD34-007F
FL-CD34-012F
FL-CD36-001F
FT001F
106
FT002F
106
100
100
100
99
99
99
99
101
H
HAF001F
105
HAF002F
105
HC001F
105
HUVEC-001 95
HUVEC-001F 95
HUVEC-001F-M
HUVEC-002 95
HUVEC-003 95
HUVEC-004 95
95
I
DIA1-FPB05
DIA1-FPB10
DIA1-FPB30
DIA1-PB001F
DIA1-PB011F
DIA2-FPB05
DIA2-FPB10
DIA2-FPB30
DIA2-PB001F
DIA2-PB011F
DMEM-001
DMEM-002
DP001F
DP002F
DP003F
DP004F
F
149
149
149
149
149
150
150
150
150
150
174
174
106
106
106
106
Toll Free Phone: 1-888-535-3444
IMDM-001
174
M
MBM-001F
MBM-002F
MCTX-001F
MDS-BM001F
MDS-BM003F
MM-BM001F
MM-BM003F
MM-BM004F
MM-BM005F
MM-BM006F
MM-BM011F
MM-BM012F
MM-BM013F
www.allcells.com
159
159
161
125
125
142
142
141
141
141
140
140
141
For In Vitro Research Use Only
194
Catalog # Index - MM-PB
ALLCELLS
MS-PB001F
MS-PB011F
MSC-001F
MSC-001F-M
MSC-002
MSC-003
MSPL-001F
MSPL-002F
MSPL-011F
MSPL-012F
MSPL-013F
145
145
78
78
78
78
162
162
163
163
163
Index: By Catalog #
MM-BM014F 141
MM-BM015F 141
MM-PB001F 139
MM-PB003F 139
MM-PB011F 138
MM-PB012F 138
MMSC-001F 160
MOCR-001F 161
MOHP-001F 161
mPB001
63
mPB002
63
mPB003
63
mPB004F
63
mPB005F
63
mPB006F
63
mPB007F
63
mPB008F
63
mPB009
65
mPB010
65
mPB011
65
mPB012
65
mPB013
65
mPB014F
65
mPB015F
65
mPB016F
65
mPB017F
65
mPB018F
65
mPB021F
64
mPB023-1
66
mPB023-2
66
mPB023-3
66
mPB023-4
66
mPB023-5
66
mPB024F-1
66
mPB024F-2
66
mPB024F-3
66
mPB024F-4
66
mPB024F-5
66
mPB025-Moz.A
mPB025-Reg.A
mPB025-Reg.E
mPB026
62
mPB033-1
67
mPB033-2
67
mPB033-3
67
mPB033F-1
67
mPB033F-2
67
mPB033F-3
67
mPB034
68
mPB034F
68
MS-FPB05
145
MS-FPB10
145
MS-FPB30
145
N
NHP-BM001
NHP-BM002
NHP-BM004
NHP-PB001
NHP-PB002
158
158
158
158
158
O
OA-FPB05
OA-FPB10
OA-FPB30
OA-PB001F
OA-PB011F
146
146
146
146
146
P
62
62
62
Toll Free Phone: 1-888-535-3444
PB001
PB002
PB003F
PB004F
PB005F
PB006F
PB007
PB007-1
PB007-2
PB007-3A
PB007-3B
PB009-1
PB009-1F
PB009-2
PB009-2F
PB009-3
PB009-3F
PB009-4
PB009-4F
PB009-5
PB009-5F
PB009-6
PB009-6F
PB009-7
PB009-7F
PB010
www.allcells.com
39
39
39
39
39
39
36
36
36
36
36
44
44
45
45
49
49
46
46
47
47
50
50
48
48
41
For In Vitro Research Use Only
195
Catalog # Index - PB-RNA
Index: By Catalog #
PB010-3
PB010-3F
PB010-4
PB010-4F
PB010F
PB010-P
PB010-P-F
PB011
PB011F
PB011-P-1
PB011-P-2
PB011-P-3
PB011-P-F-1
PB011-P-F-2
PB011-P-F-3
PB012
PB012F
PB012-P
PB012-P-F
PB013
PB013F
PB014
PB014F
PB015
PB015F
PB016
PB017
PB017F
PB018
PB018F
PB019
PB019F
PB020
PB020F
PB021
PB021F
PB022
PB022F
PB023
PB023F
PB025
PB026
PB027
PB028
PB029
PB030
PB031
PB032-1
PB032-2
PB033F-1
PB033F-2
PB033F-3
PB-DC001F
ALLCELLS
42
42
43
43
41
41
41
58
58
58
58
58
58
58
58
52
52
52
52
51
51
53
53
54
54
55
38
38
38
38
38
38
38
38
38
38
38
38
38
38
57
57
57
57
56
56
56
40
40
40
40
40
59
Toll Free Phone: 1-888-535-3444
PB-DC002F
59
PB-DC003F
59
PB-DC004F
59
PB-DC005F
59
PB-MDM-001F
60
PB-MDM-002
60
PB-MDM-003
60
PORC-PB001F
172
PORC-PB002F
172
PORC-PB003F
172
PSO-FPB05
147
PSO-FPB10
147
PSO-FPB30
147
PSO-PB001F 147
PSO-PB011F 147
R
RA-FPB05
148
RA-FPB10
148
RA-FPB30
148
RA-PB001F
148
RA-PB011F
148
RACR-001F
167
RAHP-001F
167
RBM-001F
164
RBM-002F
164
RBM-003F
166
RBM-004F
166
RBM-005F
165
RBM-006F
165
RBM-007F
165
RCTX-001F
167
RMGC-001F 168
RNA-ALL-BM001C
RNA-ALL-BM002C
RNA-ALL-PB001C
RNA-ALL-PB002C
RNA-AML-BM001C
RNA-AML-BM002C
RNA-AML-PB001C
RNA-BM001C 70
RNA-BM002C 71
RNA-BM003C 73
RNA-BM004C 74
RNA-BM005C 77
RNA-BM006C 76
RNA-BM011C 78
RNA-BM013C 75
RNA-CB001C 81
RNA-CB002C 90
RNA-CB003C 89
RNA-CB004C 88
RNA-CB006C 91
www.allcells.com
118
118
115
115
123
123
121
For In Vitro Research Use Only
196
Catalog # Index - RNA-WB
ALLCELLS
92
87
82
83
99
99
99
100
100
100
98
101
97
159
160
63
67
68
65
66
162
163
163
163
39
44
41
58
52
51
45
49
55
59
57
46
47
50
48
42
43
60
167
167
164
165
167
170
171
171
171
169
Toll Free Phone: 1-888-535-3444
RNA-WB001C
RPMI-001
174
RSPL-001F
170
RSPL-002F
170
RSPL-011F
171
RSPL-012F
171
RSPL-013F
171
37
S
SCA-FPB20
SCA-PB001F
SCA-PB011F
SLE-FPB05
SLE-FPB10
SLE-FPB30
SLE-PB001F
SLE-PB011F
SD-ILT-001
SD-ILT-002
SD-ILT-003
151
151
151
144
144
144
144
144
169
169
169
Index: By Catalog #
RNA-CB007C 93
RNA-CB008C
RNA-CB014C
RNA-CB015C
RNA-CB016C
RNA-FL001C
RNA-FL002C
RNA-FL003C
RNA-FL004C
RNA-FL005C
RNA-FL006C
RNA-FL007C
RNA-FL008C
RNA-FL010C
RNA-MBM-001C
RNA-MMSC-001C
RNA-mPB001C
RNA-mPB004C
RNA-mPB005C
RNA-mPB009C
RNA-mPB010C
RNA-MSPL-001C
RNA-MSPL-011C
RNA-MSPL-012C
RNA-MSPL-013C
RNA-PB001C
RNA-PB002C
RNA-PB003C
RNA-PB004C
RNA-PB005C
RNA-PB006C
RNA-PB007C
RNA-PB008C
RNA-PB011C
RNA-PB013C
RNA-PB014C
RNA-PB015C
RNA-PB016C
RNA-PB017C
RNA-PB018C
RNA-PB020C
RNA-PB021C
RNA-PB-MDM-001C
RNA-RACR-001C
RNA-RAHP-001C
RNA-RBM-001C
RNA-RBM-005C
RNA-RCTX-001C
RNA-RSPL-001C
RNA-RSPL-011C
RNA-RSPL-012C
RNA-RSPL-013C
RNA-SD-ILT-C
U
UC-FPB05
UC-FPB10
UC-FPB30
UC-PB001F
UC-PB011F
154
154
154
154
154
W
WB001
WB002
WB003
WB004
WB005
www.allcells.com
37
37
37
37
37
For In Vitro Research Use Only
197
NOTES
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
198
NOTES
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
199
QUICK REFERENCE GUIDE
Donor Criteria
•
Age: 18-55 years old (18-40 years old for mobilized donation)
•
Donors cannot be pregnant
•
Weight: >110 lbs for 25mL and 50mL BM donations
•
Donors are screened for HIV, Hepatitis B and Hepatitis C
•
Weight: >130 lbs for 100mL BM donations
•
Weight: >140 lbs for Normal and Mobilized Leuko Pak donation
•
CBC lab tests must meet protocol specifications
•
Donors must be in general good health. Donors with any of the
before donation
•
Donors are required to sign a procedure-specific consent form
prior to the collection procedure
•
following will be excluded from donating:
All consents and protocols have been approved by an independent biomedical review board
–History of heart, lung, liver, kidney disease (including asthma)
•
Minimum 12 months between mobilized donations
–Blood and bleeding disorders, including sickle cell disease
•
Twice in a lifetime maximum for mobilized donations
–Neurologic disorders
–Cancer
–Diabetes
–Positive: HIV, HbsAg, HCAb
Amount of White Blood Cells (WBC) in Bone Marrow
Tube Size
Median Number of WBC
Range of WBC
3ml
0.64x108
0.54-0.75x 108
10ml
2.15x108
2.05-2.25x 108
25ml
5.37x10
8
5.28-5.48x 108
50ml
1.08x109
1.07-1.09x 109
100ml
2.15x10
2.14-2.16x 109
9
The above table is for reference purposes only. In bone marrow, mononuclear cells
constitute approximately 20 to 30% of the total white blood cells. AllCells is not
responsible for the MNC or other purified cell recovery. For a specific number of a
certain cell type, please consider our bone marrow purified products.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
200
Quick Reference Guide
Cell Subset Products
The table below is for reference purposes only. Please note that the average percentage of cell subsets is referring to the recovered MNC population and not
the total white blood cell population.
Cells
NPB Average Percentage
of Cell Subsets
MPB Average Percentage
of Cell Subsets
CD34+
0.2%
BM Average Percentage
of Cell Subsets
CB Average Percentage
of Cell Subsets
0.8%
2.5%
2.5%
CD133+
0.6%
1.1%
4.1%
CD33
35%
45%
+
CD105+
0.7%
+
CD56
7%
6%
CD19+
9%
5%
CD16
16%
CD15+
0.11%
+
CD14
15%
25%
CD8+
23%
6%
+
CD4
30%
18%
CD3+
60%
33%
+
34%
24%
45%
7.8%
6%
7.8%
24%
34%
CD4+/CD45RA+
0.7%
11%
Basophils
0.3%
Neutrophils and Eosinophils
0.2%
10%
10%
RBC
Granulocytes
<0.01%
<0.01%
<0.01%
Platelets
<0.01%
<0.01%
<0.01%
CD138+ Plasma Cells
0.1-0.4%
We cannot guarantee the recovery of the cell types listed above. For a specific number of a certain cell type, please consider our purified products.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
ALLCELLS
®
www.allcells.com
[email protected]
Distributor Information
AllCells, LLC
Japan:
Spain/France:
1301 Harbor Bay Pkwy, Ste 200
Veritas Corp.
CliniSciences
Alameda, California 94502
www.veritastk.co.jp
www.clinisciences.com
1-888-535-3444
[email protected]
[email protected]
510-521-2600
03-3593-3211
33-9-77- 40-09-09
Fa x: 510-521-7600
Fa x: 03-3593-3216
Fa x: 33-9-77- 40-10-11
AllCells Shanghai
Wako Pure Chemical
Industries, Ltd.
Germany/Austria/
Switzerland:
www.wako-chem.co.jp
CellSystems
Shanghai Ju Ke Science Park
333 Guiping Road
Building 7, 2nd Floor
[email protected]
Xuhui District, 200233
81-120-052-099
Shanghai, China
[email protected]
Fa x: 81-120-052-806
49-2241-255-15
021-6485-3421
UK/Ireland:
Follow Us
Caltag
Medsystems, Ltd.
www.caltagmedsystems.co.uk
[email protected]
44-(0)-1280-827460
Fa x: 44-(0)-1280-827466
www.cellsystems.de
Fa x: 49-2241-25515-30