Freeware DNA analysis software pDRAW32 Will perform virtual cloning and PCR for you! Plots and prints linear or circular plots with restriction and primer sites Optionally plots local GC-content Also displays and prints sequence, open reading frames, primer and restriction analysis, and virtual gel. Calculates the positions and fragment sizes also of restriction enzyme mixtures. ORFs may be translated using any of 16 different codon tables Comprehensive, yet intuitive, options for selecting restriction enzymes. Perhaps best of all, it is free. Install and join the beta-tester program now To see more and download - visit http://www.acaclone.com AcaClone Software [email protected] Freeware DNA analysis software pDRAW32 AatII - 7699 - G_ACGT'C AseI - 6949 - AT'TA_AT SnaBI - 1044 - TAC'GTA p am 2µ Plots may be exported as Windows Metafiles which may be imported into PowerPoint or other similar graphic software for further manipulation. HpaI - 1686 - GTT'AAC ori ClaI - 2267 - AT'CG_AT A3 PvuII - 5477 - CAG'CTG BclI - 304 - T'GATC_A - dam methylated! MfeI - 390 - C'AATT_G XbaI - 703 - T'CTAG_A UR tet EagI - 4348 - C'GGCC_G Sal I - 4060 - G'TCGA_C SphI - 3975 - G_CATG'C Sequence may be displayed optionally either single or double stranded. Restriction sites and oligonucleotide primer sites may be optionally display along side the sequence as well as protein translation optionally in forward, reverse, all or only open reading frames SbfI - 2481 - CC_TGCA'GG NcoI - 2704 - C'CATG_G PspOMI - 2873 - G'GGCC_C ApaI - 2877 - G_GGCC'C - dcm methylated! XmaI - 3378 - C'CCGG_G SmaI - 3380 - CCC'GGG NheI - 3638 - G'CTAG_C BamHI - 3784 - G'GATC_C Readily analyses very long sequences, such as yeast chromosomes, on standard PC’s with only 64 MB RAM. Finds oligonucleotide priming sites using user database. Highlights any mismatches relative to template sequence and calculates annealing temperatures both of perfect and partial match as well as optimum annealing temperature for PCR AcaClone Software To see more and download- visit http://www.acaclone.com [email protected]
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