Appendix
Baird Parker Agar
Actinomycete agar
Sodium Caseinate
Sodium Propionate
MgSO4 7H2O
Asparagine
Dipotassium
Phosphate
Ferrous Sulfate
Agar
pH
2.0 g
4.0 g
0.1 g
0.1 g
0.5 g
0.001 g
15.0 g
8.1
3.0 g
0.2g
0.2g
0.2g
0.1g
0.01g
10g
1000ml
Peptone
Lactose
K2HPo4
Methylene Blue
Eosine Y
Agar
Distilled water
pH
10.0g
10.0g
2.0g
0.065g
0.4g
15.0g
1000ml
7.1
Glucose aspergine medium for
Siderophore production
Ashby’s medium
Mannitol
K2HPO4
MgSO4 7H2O
NaCl
Di-potassium sulfate
CaCO3
Agar
Distilled water
10.0g
5.0g
1.0g
100 mg
50 ml
5g
10 g
12 g
20g
1000ml
6.8
Eosin Methylene Blue Agar
Ammonification medium
KH2PO4
Potassium chloride
MgSO4 7H2O
Sodium chloride
Calcium sulfate
Ferrous sulfate
Peptone
Distilled water
pH
Casein Peptone
Meat Extract
Yeast Extract
K-Tellurite
Egg Yolk
Li-Chloride
Na-Pyruvate
Glycine
Agar
Distilled water
pH
20g
0.2g
0.2g
0.2g
0.1g
5g
15g
1000ml
L- Aspergine
Glucose
Na2PO4
KH2PO4
MgSO4.7H2O
pH
2
7
0.96
0.44
0.2
7
King’s B media for Pseudomonas
Peptone
K2HPo4
MgSO4 7H2O
Glycerol
Agar
20.0g
1.5g
1.5g
10 ml
10.0g
Kliegler Iron Agar
Beef extract
Yeast extract
Casein enzymic hydrolysate
Sodium chloride
Lactose
Glucose anhydrous
Ferrous ammonium sulfate
Sodium thiosulfate
pentahydrate
Phenol
red
Agar
Distilled water
3g
3g
20g
5g
10g
1g
0.5g
0.5g
0.025g
15g
1000ml
Luria- Bertani (LB) agar medium
Bacto-tryptone
yeast extract
Sodium chloride
Agar
pH
10.0g
5.0g
10.0g
15.0g
7.5
Martins Rose Bengal agar
Glucose
Peptone
K2HPO4
KH2PO4
MgSO4 7H2O
Rose bengal
Agar
Yeast extract
Streptomycin sulfate
Distilled water
pH
10 g
5g
0.5 g
0.5 g
0.5 g
30 mg
12 g
0.5 g
30 mg
1000ml
6.0-6.5
Media for Pectinolytic organisms
NaNo3
KCl
MgSO4
K2H PO4
FeSo4 7H2O
Pectin
Agar- Agar
Distilled water
pH
1g
0.5g
0.5g
1g
0.01g
10g
20g
1000ml
6.5-7.0
Media for Lignolytic organisms
MacConkey's Agar
Peptone
Lactose
Proteose peptone
Bile salts
Sodium Chloride
Neutral red
Agar
Distilled water
pH
17g
10g
3.0g
1.5g
5.0g
0.03g
15g
1000ml
7.1
NaNo3
KCl
MgSO4 7 H2O
K2HPO4
FeSO4 7H2O
Lignosulphate
Agar- Agar
Distilled water
pH
0.5g
0.5g
0.5g
1g
0.01g
10g
20g
1000ml
6.5-7.0
Minimal cellulose agar for cellulolytic
organisms
Yeast extract
KH2Po4
MgSo4
Carboxymethylcellulose
Agar- Agar
Distilled water
pH
20.0 g
10.0g
5.0g
5.0g
15.0g
1000ml
6.5-7.0
Nitrogen - free malate medium
Malic acid
K2HPO4
KOH
MgSO4
FeSO4
CaCl2
NaCl
Na2MoO4
Bromothymol blue
Agar
Distilled water
pH
5g
0.5g
4g
0.1g
0.05g
0.01g
0.02g
0.002g
2ml
20g
1000ml
6.9-7.3
Pikovskaya’s medium
Glucose
Yeast extract
(NH4)2SO4
KCl
MgSO4.7H2O
FeSO4.7H2O
Ca3(PO4)2
Sodium chloride
Distilled water
pH
Soil extract agar
Glucose
Yeast extract
K2HPO4
Agar- Agar
Soil extract (Prepared by
boiling one litre of water
mixed with one Kg of
soil followed by
filtration)
pH
1.0g
5.0g
0.2g
15.0g
1000ml
7.0
Starch agar for amylolytic organisms
Yeast extract
KH2Po4
MgSo4
Starch
Agar- Agar
Distilled water
pH
20.0 g
10.0g
5.0g
5.0g
15.0g
1000ml
7.0
Yeast Extract Mannitol Agar (YEMA)
10g
0.05g
0.05g
0.2g
0.1g
Traces
5g
0.2g
1000ml
7.0 –
7.2
KH2PO4
K2SO4.7H2O
NaCl
Mannitol
Yeast extract
Agar
Congo red (1% aqueous
solution) water
Distilled
pH
0.5g
0.2g
0.1g
10g
1g
20g
2.5ml
1000ml
7.0