Manya Warrier, Ph.D.
769 Franklin Tree Drive
Concord, NC 28027
Phone: 980-621-0823
Email: [email protected]/[email protected]
Education
§ Ph.D. in Biomedical Sciences, The University of Toledo, Toledo, Ohio
2002 - 2008
o Concentration: Cardiovascular and Metabolic Diseases
o Advisor: Dr. Edwin R. Sanchez; Co-advisor: Dr. Sonia M. Najjar
o Dissertation: Differential Control of Metabolism by Steroid Receptor Co-chaperones FKBP2 and
FKBP51
§ M.Sc. in Biotechnology, University of Mysore, India
1996-1998
o Thesis: Studies on Micropropagation, Callus induction and Protoplast Isolation of Capsicum
(Capsicum annuum L.)
o Standardized and developed protocols for micropropagation, callus induction and protoplast
isolation of Capsicum.
§ B.Sc. in Biochemistry, Bharathiar University, India
1993-1996
Research Experience
§ Research Assistant Professor, Nutrition Research Institute, UNC Chapel Hill, Kannapolis, NC
2015 - Current
o Research: Exploring factors that regulate adipose browning.
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Postdoctoral Fellow, Cleveland Clinic Foundation, Cleveland, OH / Wake Forest School of
Medicine, Winston-Salem, NC
2011- 2014
o Principal Investigator: Dr. J. Mark Brown, Assistant Staff (Cellular and Molecular Medicine)
o Projects: 1) Identify mechanisms regulating Transintestinal Cholesterol Efflux (TICE)
2) The Role of Flavin Monooxygenase 3 in Energy Metabolism
Postdoctoral Fellow, Cardiovascular Pathobiology Program, Sanford-Burnham Diabetes and
Obesity Research Center, Orlando, FL
2008 - 2010
o Principal Investigator: Dr. Daniel P. Kelly, Professor and Scientific Director
o Project: Understand the biology of a spliced isoform of PGC-1α in heart.
Research Trainee, Pharmacology, The University of Toledo, Toledo, OH
2001-2002
o Principal Investigator: Dr. William S. Messer, Professor and Chair
Research Trainee, Indian Institute of Spices Research (IISR), India
1999-2000
o Principal Investigators: Dr. P.N. Ravindran and Dr. K. Nirmal Babu
Academic and Professional Honors
§ Pre-doctoral Research Fellowship, The University of Toledo, College of Medicine (2002-2008)
§ Student Travel Grant from Graduate School (2006, 2008)
§ Keystone Symposia Scholarship (2008)
§ Winner of Postdoctoral Scholar Development Award (Wake Forest University; 2012)
§ Recipient of Atherosclerosis Thrombosis Vascular Biology (ATVB) Travel Award for Young
Investigators (2013)
§ Invited Talk, ATVB Conference, Orlando, FL (2013)
§ Recipient of a Postdoctoral Fellowship from the American Heart Association (2014)
§ Early Career Investigator Travel Award (Kern Lipid Conference; 2014).
§ Junior Faculty Development Award from UNC-Chapel Hill (2015)
§ Murdock - NCRC Seed Fund (2015)
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Publications (Published/In Preparation)
1. Stechschulte LA, Qiu B, Warrier M, Hinds TD Jr, Zhang M, Gu H, Xu Y, Khuder SS, Russo L, Najjar
SM, Lecka-Czernik B, Yong W, Sanchez ER (2016), ‘FKBP51 Null Mice Are Resistant to Diet-Induced
Obesity and the PPARγ Agonist Rosiglitazone’, Endocrinology. 2016 Jul 21.
2. Warrier M, Zhang J, Bura K, Kelley K, Wilson MD, Rudel LL, Brown JM (2016), ‘Sterol OAcyltransferase 2-Driven Cholesterol Esterification Opposes Liver X Receptor-Stimulated Fecal Neutral
Sterol Loss’, Lipids. Feb; 51(2): 151-7.
3. Warrier M, Shih DM, Burrows AC, Ferguson D, Gromovsky AD, Brown AL, Marshall S, McDaniel
A, Schugar RC, Wang Z, Sacks J, Rong X, Vallim TA, Chou J, Ivanova PT, Myers DS, Brown HA,
Lee RG, Crooke RM, Graham MJ, Liu X, Parini P, Tontonoz P, Lusis AJ, Hazen SL, Temel RE,
Brown JM. (2015), ‘The trimethylamine-N-oxide generating enzyme flavin monooxygenase 3 (FMO3)
is a novel regulator of cholesterol balance and reverse cholesterol transport’, Cell Reports, Jan 14.
4. Koeth RA, Wang Z, Levison BS, Buffa JA, Org E, Sheehy BT, Britt EB, Fu X, Wu Y, Li L, Smith JD,
DiDonato JA, Chen J, Li H, Wu GD, Lewis JD, Warrier M, Brown JM, Krauss RM, Tang WH,
Bushman FD, Lusis AJ, Hazen SL. (2013), ‘Intestinal microbiota metabolism of L-carnitine, a nutrient
in red meat, promotes atherosclerosis’, Nature Medicine. 19(5): 576-85.
5. Bura KS, Lord C, Marshall S, McDaniel A, Thomas G, Warrier M, Zhang J, Davis MA, Sawyer JK,
Shah R, Wilson MD, Dikkers A, Tietge UJ, Collet X, Rudel LL, Temel RE, Brown JM. (2013),
‘Intestinal SR-BI does not impact cholesterol absorption or transintestinal cholesterol efflux in mice’,
Journal of Lipid Research. 54(6): 1567-77.
6. Saddar S, Carriere V, Lee WR, Tanigaki K, Yuhanna IS, Parathath S, Morel E, Warrier M, Sawyer
JK, Gerard RD, Temel RE, Brown JM, Connelly M, Mineo C, Shaul PW. (2013), ‘Scavenger receptor
class B type I is a plasma membrane cholesterol sensor’, Circulation Research.112 (1): 140-51.
7. Warrier M, Hinds TD Jr, Ledford KJ, Cash HA, Patel PR, Bowman TA, Stechschulte LA, Yong W,
Shou W, Najjar SM, Sanchez ER. (2010), ‘Susceptibility to diet-induced hepatic steatosis and
glucocorticoid resistance in FK506-binding protein 52-deficient Mice’, Endocrinology. 151(7): 322536.
8. Periyasamy S, Warrier M, Tillekeratne MP, Shou W, Sanchez ER. (2007), ‘The Immunophilin ligands
cyclosporin A and FK506 suppress prostate cancer cell growth by androgen receptor-dependent and independent mechanisms’, Endocrinology, 48(10): 4716-26.
9. Tejada FR, Nagy PI, Xu M, Wu C, Katz T, Dorsey J, Rieman M, Lawlor E, Warrier M, Messer WS Jr.
(2006), ‘Design and synthesis of novel derivatives of the muscarinic agonist tetra (ethyleneglycol)(3methoxy-1,2,5-thiadiazol-4-yl)[3-(1-methyl-1,2,5,6-tetrahydropyrid-3-yl)-1,2,5-thiadiazol- 4-yl] ether
(CDD-0304): effects of structural modifications on the binding and activity at muscarinic receptor
subtypes and chimeras’, Journal of Medicinal Chemistry, 49(25):7518-31.
10. Warrier M, Schugar RC, Burrows A, Ferguson D, Brown AL, Gromovsky AD, Chatterjee A, Li L, Li
X, Wang Z, Willard B, Richard G. Lee, Rosanne M. Crooke, Mark J. Graham, Richard E. Morton, Carl
D. Langefeld, Swapan K. Das, Lawrence L. Rudel, Nizar Zein, Arthur J. McCullough, Srinivasan
Dasarathy, W.H. Wilson Tang, Markku Laakso, Mete Civelek, Aldons J. Lusis, Sathyamangla V. Naga
Prasad, Stanley L. Hazen, and J. Mark Brown, Postprandial Gut Microbiota-Driven Choline Metabolism
Links Dietary Cues to Obesity-Induced Insulin Resistance in Humans (Submitted to Cell Metabolism –
Under Revision)
Published Abstracts
1. K. N Babu, S.P.Geetha, D. Minoo, W. Manya, P.Girija, P.N. Ravindran and K.V. Peter (1998), ‘In
Vitro Approaches for Conservation and Exchange of Spices Germplasm’, Golden Jubilee National
Symposium – Spices, Medicinal And Aromatic Plants: Biodiversity, Conservation and Utilisation (NSSMAP), Calicut, Kerala, India.
2. Manya Warrier, Edwin R. Sanchez (2006), ‘Stress Regulation of Glucocorticoid Receptor’, The
Endocrine Society’s 88th Annual Meeting, Boston, MA, USA.
3. Manya Warrier, Stephanie Marshall, Allison McDaniel, Martha Wilson, Amanda Brown, Zeneng
Wang, Diana Shih, Aldons Jake Lusis, Richard Lee, Stanley Hazen, Ryan E Temel, J Mark Brown,
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‘Flavin Monooxygenase 3 (FMO3) is a Novel Regulator of Hepatic Cholesterol Metabolism and
Transintestinal Cholesterol Efflux (2013). Atherosclerosis, Thrombosis and Vascular Biology
Conference, Orlando, Florida, USA.
Poster Presentations
1. Manya Warrier, Edwin R Sanchez (2003), ‘Effect of Endogenous Ligands on Stress-Induced
Glucocorticoid Receptor Transactivation’, The 1st Annual Great Lakes Nuclear receptor Conference,
Medical College of Ohio; Toledo, Ohio
2. Manya Warrier, Edwin R Sanchez (2005), ‘Stress Induced Glucocorticoid Receptor Signaling: Role in
Endogenous Systems’, 32nd Pharmacology Research Colloquium, Wayne State University, School of
Medicine, Detroit, MI.
3. Manya Warrier, Edwin R. Sanchez (2005), ‘Ligand and Promoter Specificity in Stress Induced
Glucocorticoid Receptor Signaling’, 20th Annual College of Graduate Studies Research Forum, Medical
College of Ohio, Toledo, Ohio.
4. Manya Warrier, Edwin R. Sanchez (2006), ‘Stress Regulation of Glucocorticoid Receptor
Signaling’, 33rd Pharmacology Research Colloquium, Michigan State University, East Lansing, MI.
5. Manya Warrier, Edwin R. Sanchez (2006), ‘Stress Regulation of Glucocorticoid Receptor
Signaling’, 21st Annual College of Graduate Studies Research Forum, Medical University of Ohio,
Toledo, Ohio.
6. Manya Warrier, Edwin R. Sanchez (2006), ‘Stress Regulation of Glucocorticoid Receptor’, The
Endocrine Society’s 88th Annual Meeting, Boston, MA.
7. Manya Warrier, Weinian Shou, Sonia Najjar, Edwin R. Sanchez (2007), ‘Role of TPR Proteins in
Glucocorticoid Regulated Metabolism’, The 3rd Biannual Great Lakes Nuclear Receptor Conference,
University of Pittsburgh, Pittsburgh, PA.
8. Sumudra Periyasamy, Manya Warrier, Mano Tillekeratne, Edwin R. Sanchez (2007), ‘The
Tetratricopeptide Repeat Protein Ligands Cyclosporine A and FK506 Regulate Androgen Receptor
Function in Prostate Cancer Cells’, The 3rd Biannual Great Lakes Nuclear Receptor Conference,
University of Pittsburgh, Pittsburgh, PA.
9. Manya Warrier, Payal Patel , Mats A. Fernström, Weidong Yong , Anthony M. DeAngelis, Weinian
Shou, Sonia M. Najjar, Edwin R. Sanchez (2008), ‘Differential control of lipid and carbohydrate
metabolism by the glucocorticoid receptor co-chaperones FKBP51 AND FKBP52’, Keystone Symposia
on Molecular Biology (Steroid Sisters), Whistler, British Columbia, Canada.
10. Manya Warrier, Steven Ewer, Ling Lai, Teresa C. Leone, Daniel P. Kelly (2010), ‘ NT-PGC-1α, an
Alternatively Spliced Form of PGC-1α, Co-localizes with and Activates the Estrogen-Related (ERRα)
Receptor in Cardiac Myocytes’, Frontiers in Biomedical Science, Inaugural Scientific Symposium,
Sanford-Burnham Medical Research Institute at Lake Nona, Orlando, Fl.
11. Manya Warrier, Steven Ewer, Gregory Aubert, Teresa C. Leone, Daniel P. Kelly (2011), ‘NT-PGC1α, an Alternatively Spliced Form of PGC-1α, Co-localizes with and Activates the Estrogen- Related
Receptor (ERRα)’, Keystone Symposia on Type 2 Diabetes, Insulin Resistance and Metabolic
Dysfunction, Keystone, Colorado.
12. Manya Warrier, Jun Zhang, Kathryn Kelly, Martha D Wilson, Lawrence L Rudel, Ryan E Temel, J
Mark Brown (2012), ‘Acyl-CoA:Cholesterol Acyltransferase (ACAT2) is a Negative Regulator of
LXR-Stimulated Reverse Cholesterol Transport’, Gordon Research Conference on Lipoprotein
Metabolism, Waterville Valley Resort, NH.
13. Manya Warrier, Stephanie Marshall, Allison McDaniel, Martha D Wilson, Ryan E Temel, J Mark
Brown (2013), ‘Flavin Monooxygenase 3 (FMO3) is a Novel Regulator of Hepatic Cholesterol
Metabolism’, 13th Graduate Student and Postdoc Research Day, Wake Forest University, WinstonSalem, NC.
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14. Manya Warrier, Jun Zhang, Kathryn Kelly, Martha D Wilson, Lawrence L Rudel, Ryan E Temel, J
Mark Brown (2013), ‘Acyl-CoA:Cholesterol Acyltransferase (ACAT2) is a Negative Regulator of
LXR-Stimulated Reverse Cholesterol Transport’, LRI Retreat, Cleveland, OH.
15. Manya Warrier, Stephanie Marshall, Allison McDaniel, Martha D Wilson, Ryan E Temel, J Mark
Brown (2013), ‘Flavin Monooxygenase 3 (FMO3) is a Novel Regulator of Hepatic Cholesterol
Metabolism’, Cleveland Clinic Research Day, Cleveland, OH.
16. Manya Warrier, Stephanie Marshall, Zeneng Wang, Rosanne M Crooke, Stanley Hazen, J Mark
Brown (2014), ‘Flavin Monooxygenase 3 (FMO3) is a Novel Regulator of Hepatic Cholesterol
Metabolism’, Kern Lipid Conference, Vail, Colorado.
Invited Seminars and Talks
1. Larry E. Gentry Student Research Forum 2003, Toledo, Ohio
2. Larry E. Gentry Student Research Forum 2004, Toledo, Ohio
3. Larry E. Gentry Student Research Forum 2005, Toledo, Ohio
4. Molecular and Cellular Biology Seminar Series, 2005, Medical College of Ohio, Toledo, Ohio
5. Larry E. Gentry Student Research Forum 2006, Toledo, Ohio
6. Department of Lipid Sciences Trainee Seminar Series 2012, Winston-Salem, NC
7. Atherosclerosis Thrombosis Vascular Biology 2013, Orlando, Florida
8. Molecular and Cellular Medicine Department Trainee Seminar Series, Lerner Research Institute 2014,
Cleveland, Ohio
9. Page Lecture Workshop with Nobel Prize Laureate Dr. Joseph Goldstein, Lerner Research Institute
2014, Cleveland, Ohio
10. Trainee Round Table Workshop with Dr. Kathryn J. Moore, Lerner Research Institute 2014, Cleveland,
Ohio.
Research Skills:
o Cell Culture: Mammalian cells (fibroblasts, hepatocytes, myoblasts and brown/white adipocytes)
Insect cells
o Molecular/Cell Biology Techniques: Transient transfections and adenoviral infections,
- Reporter gene assays (Single and Dual Luciferase and
- Chloramphenicol Acetyl Transferase (CAT) reporter assays)
- DNA manipulations (Subcloning)
- DNA/RNA/Protein preparations
- Real Time / Semi-quantitative RT-PCR
- Gel Electrophoresis (SDS PAGE), Western Blots and
- Immunoprecipitation
- Immunofluorescence
- Mitotracker fluorescence assay to study mitochondrial function
Oxygen consumption in cells (using Seahorse instrument)
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o Mouse colony management: Maintained (breeding, genotyping and record keeping) several knockout
and transgenic mice colonies
o In Vivo Skills: Retro-orbital/tail/submandibular bleeding, Tissue collection
- IP/Antisense Oligonucleotide (ASO) injections, Oral gavage
- Cholesterol absorption study, Triton block to measure VLDL secretion
- Glucose/Insulin Tolerance Tests (GTT/ITT)
- In Vivo macrophage reverse cholesterol transport assay
- Thioglycollate elicitation of macrophages and collection
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- Primary mouse hepatocyte and rat neonatal cardiomyocyte preparation and culture
- Metabolic cage measurements
o Biochemical Assays: Extract and measure lipids and bile acids from plasma, bile and tissues
- Bile acid pool size and composition measurement
- Fecal neutral and acidic sterol measurements
- Phospholipid extraction from tissues (preparation for lipidomics)
- Radioactive lipid tracing in primary hepatocytes
- Enzyme - Linked Immunosorbent Assay (ELISA)
o Others: Isolation of lipoproteins from human plasma and cholesterol efflux studies using primary
macrophages.
- Tissue sectioning and histological staining
External Funding
§ AHA Postdoctoral Fellowship
Total Cost $48,000 (Great Rivers Affiliate)
1/1/2014 – 12/31/2014
Professional Service
§ Reviewer: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy
Journal of Receptor, Ligand and Channel Research
Research and Reports in Endocrine Disorders
Professional Memberships
§ Member, The Endocrine Society: 2003 –2010
§ Member, American Heart Association: 2011- Current
References: Upon Request
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