Universal Plus mRNA-Seq
www.nugen.com
Expand your ability to do mRNA-Seq
Universal Plus mRNA-Seq is
a comprehensive solution
for standard mRNA-seq
studies with a vastly simplified
workflow. As a novel feature,
Universal Plus mRNA-Seq
incorporates the ability to
eliminate unwanted transcripts
from mRNA-seq libraries using
the proprietary AnyDeplete
technology. This strategy,
typically employed for whole
transcriptomics studies,
expands the data that can be
obtained from an mRNA-seq
experiment.
+
AAAAA
oligoT
beads
Features
Universal Plus mRNA-Seq has
several unique features:
1. Broad dynamic input range,
from 1 μg to as low as 10 ng,
enables access to previously
inaccessible low input
samples.
2. Library construction, that
includes the proprietary
DimerFree technology, allows
for more efficient and robust
library preparation.
3. Customizable transcript
depletion with AnyDeplete
maximizes informative data
from mRNA sequencing.
cDNA
Synthesis
mRNA
Selection
tRNA
rRNA
mRNA
Why use Universal Plus
mRNA-Seq?
• Broad dynamic input range
(10 ng to 1 μg)
• Random and oligo(dT)
priming
• Stranded libraries
• Efficient library preparation
without adaptor titration
• Pre-plated adapters
• Unique barcodes included
for every sample
• Customizable transcript
depletion after library
preparation
• Fully automatable
Adaptor
Ligation
Transcript
Depletion
DimerFree
AnyDeplete
A
AA
AA
AA
AA
AAAAA
A
AAAAA
AAA
AA
no adaptor
dimer
tRNA
rRNA
unwanted transcript
removal
AA
AA
A
Figure 1 Universal Plus mRNA-Seq is a complete end-to-end solution for mRNA sequencing, with a simple easy to follow workflow.
®
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100%
•
Input range: 10 ng - 1 μg total RNA
•
Even 5’-3’ transcript coverage
•
High correlation across wide input
range
•
Simplified workflow
Percent Reads
Technical details
•
Gene expression analysis
20%
10 ng 100 ng 1 μg
•
Transcript discovery
•
Splice variant and isoform analysis
•
Gene fusion detection
100%
Percent Reads
mRNA sequencing
Removes unwanted transcripts after
library preparation
Add new probes to existing probe sets
without re-optimization
Customizable to make the kit speciesspecific or experiment specific
Exon
80%
Globin
rRNA
Other
60%
40%
+ With AnyDeplete
– No AnyDeplete
20%
0%
Why use AnyDeplete?
•
40%
Figure 2 Read alignment metrics are consistent from 1 μg to
10 ng total K562 RNA inputs, allowing access to data from low
input samples. Other includes intron, intergenic, unaligned and
multialigned reads.
•
•
60%
0%
Applications
•
Exon
rRNA
Other
80%
–
Sample
Without
AnyDeplete
With
AnyDeplete
+
–
+
rRNA
globin
Exon
Genes over
FPKM > 1
2.5%
32.8%
40.9%
9771
0.1%
1.3%
70.3%
9985
Figure 3 Read alignment metrics from 100ng whole blood mRNAseq. Targeted globin transcript depletion increases the number
of reads mapping to exons, resulting in over 200 more genes
detected. FPKM based on RefSeq transcripts. Other includes
intron, intergenic, unaligned and multialigned reads.
AnyDeplete probe sets can be customized to any transcript from any organism. For kits with custom probe sets, contact your
Account Executive or request a quote on our website.
®
www.nugen.com
NuGEN Technologies, Inc.
Headquarters USA
Europe
Worldwide
201 Industrial Road, Suite 310
San Carlos, CA 94070 USA
Toll Free Tel: 888.654.6544
Toll Free Fax: 888.296.6544
[email protected]
[email protected]
P.O. Box 109,
9350 AC Leek
The Netherlands
Tel: +31-13-5780215
Fax: +31-13-5780216
[email protected]
For our international distributors
contact information, visit our website.
[email protected]
©2017 NuGEN Technologies, Inc. All rights reserved. The Encore®, Ovation® and Applause® families of Products and methods of their use
are covered by several issued U.S. and International patents and pending applications (www.nugen.com). NuGEN, Ovation, SPIA, Ribo-SPIA,
Applause, Encore, Prelude, Mondrian and Imagine More From Less are trademarks or registered trademarks of NuGEN Technologies, Inc.
Other marks appearing in these materials are marks of their respective owners.
For research use only.
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