09/10
c
a t a
l
o
tools for
MolecularBiology
g
tools for MolecularBiology
Plasmid DNA Purification
page 4
Genomic DNA Purification
page 8
Viral Nucleic Acid Isolation
page 13
PCR Clean-Up
page 14
DNA Extraction from Gels
page 16
RNA Purification
page 17
Vacuum Manifold
page 21
DNA and Protein Markers
page 22
Recombinant Screening
page 25
PCR Reagents
page 27
Electrophoresis Reagents and Stains
page 28
Plastic-ware for Sample Preparation
page 32
aComprehensivefamily
of Products for Molecular Biology research
The response to axyPrep kits has continued to be enthusiastic and
many new customers have adopted our products for their Life
Science research. axyPrep products continue to generate satisfaction
and praise from our many customers for their outstanding
performance and value. The axyPrep family of kits has been
expanded to include a full line of kits for rna purification. When
compared with the current market leaders, axyPrep kits will meet or
exceed performance specifications at a unit price which is generally
significantly lower than comparable brands.
in addition, the new 2009/10 catalog contains many new products to
accompany the axyPrep purification kits in molecular Biology
applications. These new products include: Pcr reagents, several
types of agarose, an expanded array of electrophoretic Dna and
protein markers, stains and recombinant screening kits. This catalog
contains a list of our most popular columns, spin baskets and plates
which encompass a wide array of different molecular biology
applications.
Please do not hesitate to contact us if you should ever have any
technical questions or have axygen develop a custom solution to
your application demands.
axygenBio.com
axyPrep™ Plasmid
miniPreP kiT
The axyPrep Plasmid miniprep kit
M
1
2
3
4
5
6
7
8
M
provides a raPiD
anD
economicaL meThoD for
The PurificaTion of
PLaSmiD Dna in a convenient
vacuum protocols
Lane 1 3 Kb
Lane 2 4 Kb
Lane 5 6 Kb
Lane 6 6 Kb
Lane 3 4.9 Kb
Lane 4 5 Kb
Lane 7 8 Kb
Lane 8 10 Kb
M
mini column format. each column can
I Rapid spin and
different size plasmids
purified with the axyPrep
Plasmid Miniprep
Axygen 1Kb DNA Ladder
I No alcohol
precipitation
I High yields of up
to 20 µg
I High-purity plasmid
process up to ml of bacterial culture
suitable for many
downstream
applications
grown in LB or up to 2 ml of culture
grown in rich broth. The entire
procedure can be completed
WiThin 20 minuTeS. The highly
M
purified plasmid Dna can be used
1
2
3
4
Plasmid pBluescript (with insert) purified
with the axyPrep Plasmid Miniprep Kit.
immediately for many routine
applications, such as Dna sequencing,
Lanes 1, 3
restriction digestion, in vitro
Lanes 2, 4
transcription, library screening, ligation
M
and transformation.
pBluescript
(0.4 µg/lane)
Digested with EcoRI
(0.3 µg/lane)
Mixed 5 Kb, 750 bp,
500 bp, and 100 bp
fragments
oVerVieW
sample
lysis
neutralization
spin
Binding
Washing
elution
™
aBi PrisM® 3730 data. Big dye v. 3.1.
PLASMID
MINIPREP
axygenBio.com
KIT SIZES
4 preps
50 preps
250 preps
CATALOG NO.
aP-MN-P-4
aP-MN-P-50
aP-MN-P-250
Vacuum
axyPrep™ Plasmid
miDi & maxiPreP kiTS
These kits are based upon a modified
M
1
2
3
4
SDS-alkaline lysis of bacterial cells in
combination with lysate filtration and
the selective binding of the plasmid
Dna to special axyPrep purification
columns. The protocols provide
I Rapid protocols
Plasmid puc19 purified with
the axyPrep Plasmid Midiprep
Kit. 0.4 µg/lane.
Lane 1
Lane 2
Unrestricted
Restricted with EcoR I
Lane 3
Lane 4
Restricted with Pvu II
Restricted with Taq I
M
Axygen 1Kb DNA Ladder
I No alcohol
precipitation
I High yields of up to
100 µg and 500 µg
respectively
I High-purity plasmid
SimPLe, raPiD anD efficienT
meThoDS for The
iSoLaTion of highLy
PurifieD PLaSmiD Dna. The
M
1
suitable for many
downstream
applications
2
yield of plasmid for the midiprep and
maxiprep kits is up to 100 µg and 500 µg
oVerVieW
respectively. The entire purification
processes can be completed in 30
Midi
Maxi
minutes for the midiprep, and 5
minutes for the maxiprep. Plasmid
lysis neutralization
prepared by this method will exhibit
excellent performance in most
centrifugation
applications requiring larger quantities
filtration
of highly purified plasmid, such as Dna
sequencing, restriction digestion,
bacterial transformation, etc. a vacuum
manifold and vacuum source are
required.
Binding
Plasmid pcdNa3.1 (with insert)
purified with the axyPrep Plasmid
Maxiprep Kit. 0.4µg/lane.
Lane 1
Washing
Unrestricted
Lane 2
Restricted with EcoR I
M
Axygen 1Kb DNA Ladder
elution
PLASMID
MIDIPREP
KIT SIZES
2 preps
10 preps
25 preps
CATALOG NO.
aP-Md-P-2
aP-Md-P-10
aP-Md-P-25
PLASMID
MAXIPREP
KIT SIZES
2 preps
10 preps
25 preps
CATALOG NO.
aP-MX-P-2
aP-MX-P-10
aP-MX-P-25
5
axyPrep™ 96 Plasmidkit
I High-throughput,
The axyPrep-96 Plasmid kit represents a
96-well plate format
True high-ThroughPuT
SySTem for The
PurificaTion of PLaSmiD or
coSmiD Dna in a 96-well format.
I Two-plate system
(lysate filtration and
plasmid binding)
I Up to 20 µg of highly
This product is designed to rapidly
purified plasmid per
well
produce up to 20 µg of highly purified
plasmid or cosmid Dna (per well) from
I Rapid vacuum protocol
up to 1.3 ml of bacterial culture grown
I No alcohol
in a 96-well format.
precipitation
I Automation-
Two plates rapidly and efficiently
process the bacterial lysate, using
compatible plasticware
I Suitable for automated
either vacuum or centrifugation. The
Lysate filtration Plate efficiently
removes bacterial cellular debris and
transfers the clarified lysate directly
Multiple aliquots of plasmid pBs (with 1Kb insert) grown in
JM109 purified with the axyPrep-96 Plasmid Kit. 1.3 ml of
culture processed per well. approximately 0.3 µg plasmid run
per lane on a 1% agarose gel. axygen 1Kb ladder included.
into the Plasmid Plate for desalting and
fluorescent sequencing
oVerVieW
elution. The highly-purified plasmid or
cosmid Dna is suitable for most
collection,
suspension
applications, including: automated
lysis neutralization
fluorescent sequencing on the aBi
filtration
PriSm® Dna analyzers, in vitro
transcription, restriction analysis, Pcr,
etc. a vacuum manifold and vacuum
Binding
source are required.
Washing
representative aBi PrisM® 3730 data. Big dye™ v. 3.1.
elution
Plasmid dNa
96 PLASMID
6
axygenBio.com
KIT SIZES
1x96 prep
4x96 preps
24x96 preps
CATALOG NO.
aP-96-P-1
aP-96-P-4
aP-96-P-24
axyPrep™ easy-96 Plasmidkit
I Cost-effective plasmid
The axyPrep easy-96 Plasmid kit employs
purification in a
96-well format
an optimized 96-well lysate filtration plate
and an alcohol precipitation step to
I Lysate filtration
raPiDLy anD economicaLLy
Purify PLaSmiD anD coSmiD
Dna from muLTiPLe
BacTeriaL cuLTureS. The kit is
followed by alcohol
precipitation
I Rapid vacuum and
centrifuge protocols
designed to process up to 1.3 ml aliquots
I "Molecular Biology-
of bacterial culture, grown in a 96-well
Grade" plasmid DNA
suitable for many
applications
format. plasmid or cosmid Dna prepared
by the easy-96 Plasmid kit typically
contains residual amounts of bacterial
rna and is suitable for a variety of
applications, such as automated
sequencing, restriction analysis, Pcr,
probe synthesis, etc. This kit is also
Multiple aliquots of plasmid pBs (with 1Kb insert) grown in
dh5α purified with the axyPrep easy-96 Plasmid Kit. 1.3 ml of
culture processed per well. approximately 0.5 µg plasmid run
per lane on a 1% agarose gel. axygen 1Kb ladder included.
recommended for the purification of
large recombinant constructs, such as
oVerVieW
Bacs and P1. a vacuum manifold and
single-colony cultures
vacuum source are required for vacuummediated lysate filtration.
suspension
lysis
neutralization
optional heating
step (plasmid only)
filtration
representative aBi PrisM® 3730 data. Big dye™ v. 3.1.
EASY-96
PLASMID
KIT SIZES
1x96 prep
4x96 preps
24x96 preps
Precipitation/
centrifugation
Washing
Plasmid dNa
CATALOG NO.
aP-e96-P-1
aP-e96-P-4
aP-e96-P-24
ned
esig
Red for
d
rove
Imp rmance
o
Perf
axyPrep™ Multisource GenomicDNA
miniPreP kiT
axyprep multisource genomic Dna
I Expanded protocols for
Mouse tissues
1
miniprep kits generate high yields of
2
3
4
5
6
7
8
9 10
genomic Dna from a variety of starting
sample materials. The kit has been
reDeSigneD for greaTer
eaSe-of-uSe anD
BroaDer uTiLiTy. This new
genomic dNa purified from 20 mg
of various mouse tissues with the
redesigned axyprep Multisource
genomic dNa Miniprep Kit.
lanes 1, 2: Brain
lanes 3, 4: heart
lanes 5, 6: liver
lanes 7, 8: Kidney
lanes 9, 10: Muscle
1 µg/lane
system employs a special lysis buffer
which acts in combination with
Plant tissues
M
1
2
3
4
5
M
Proteinase k to efficiently release
genomic Dna from the biologic
starting material. contaminating
proteins, pigments, carbohydrates and
genomic dNa isolated from 20 mg
of various plant tissues.
lanes 1, 2: Peanut leaves
lanes 3, 4: yam leaves
lanes 5, 6: Watermelon
leaves
200 ng/lane
animal and plant tissues,
cultured cells,
lymphocytes, bone
marrow, dried blood,
buccal swabs, yeast and
filamentous fungi
I High yields - up to 20 µg
of highly purified gDNA
I High molecular weight
gDNA, ≥30 Kb
I Rapid spin and vacuum
protocols
I No alcohol precipitation
lipids are then efficiently segregated
from the genomic Dna by a
oVerVieW
precipitation step, coupled with the
selective adsorption of the genomic
Dna to a special axyPrep column.
cultured cells
1
2
The genomic Dna produced by this kit
is exceptionally pure and
approximately 30 kb in length.
3
4
5
M
genomic dNa isolated from
cultured mammalian cells.
lanes 1:
hl-60
lanes 2:
cos-7
lanes 3:
cho
lanes 4:
hela
lanes 5:
sp20
M:
Marker
500 ng/lane
lysis
Proteinase K
digest
Neutralization
Binding
Washing
elution
MULTISOURCE
GENOMIC DNA
axygenBio.com
KIT SIZES
CATALOG NO.
4 preps
aP-MN-Ms-gdNa-4
50 preps
aP-MN-Ms-gdNa-50
250 preps
aP-MN-Ms-gdNa-250*
*shipped as AP-MN-MS-GDNA-50
axyPrep™Blood
Genomic
DNA
miniPreP kiT
The axyPrep Blood genomic Dna
M
1
2
3
4
5
6
M
I For fresh, frozen or dried
blood samples
miniprep kit is suitable for
I Up to 12 µg of highly
Purifying uP To 12 µg* of
genomic Dna from 250 µl
of WhoLe BLooD. genomic
purified genomic DNA
I Rapid spin protocol
I No alcohol precipitation
Dna is isolated directly from the white
blood cell (WBc) component of whole
I High molecular weight
blood, without the need to first remove
genomic DNA product
(≥30 Kb)
the red blood cells (rBcs). The isolation
of genomic Dna from blood by this
method is based on the efficient release
Lanes 1, 3, 5
Unrestricted
of genomic Dna by a special cell lysis
Lanes 2, 4, 6
Restricted with EcoRI
M
Axygen 1Kb DNA Ladder
and heme/protein precipitation buffer,
coupled with the selective adsorption of
genomic dNa purified
from anticoagulated
human blood samples
with the axyPrep Blood
genomic dNa Miniprep
Kit. 0.4 µg/lane.
I Purified genomic DNA
is suitable for many
downstream applications
the genomic Dna to a special axyPrep
column. The purified Dna is
oVerVieW
predominantly ≥30 kb in length, and is
M
1
2
3
4
5
M
suitable for a variety of applications
sample
demanding highly purified, high
molecular weight genomic Dna, such as
lysis
Southern blot analysis, Pcr, etc.
Precipitation
*Yields of approximately 10-12 µg per prep
are achievable when processing 500 µl of
human whole blood, whole blood with
elevated leukocyte levels or non-human
blood. Routine yields of approximately
5-6 µg are anticipated when processing
250 µl of normal human blood.
BLOOD
GENOMIC DNA
genomic dNa purified from
clotted and dried human blood
samples. 0.3 µg/lane.
Lanes 1-3
Clotted
Lanes 4-5
Dried
M
Marker
Binding
Washing
elution
KIT SIZES
4 preps
50 preps
250 preps
CATALOG NO.
aP-MN-Bl-gdNa-4
aP-MN-Bl-gdNa-50
aP-MN-Bl-gdNa-250
9
axyPrep™Blood
Genomic
DNA
miDi & maxiPreP kiTS
The axyPrep Blood genomic Dna midi
M
1
2
3
4
5
6
I Rapid protocols for
M
fresh or frozen
anticoagulated blood
samples
and maxiprep kits are designed to
ProDuce uP To 100 µg anD
250 µg of high moLecuLar
WeighT genomic Dna
reSPecTiveLy from
anTicoaguLaTeD human
or animaL BLooD. Purification is
I Unique phase-partition
step for higher yield
I No alcohol
precipitation
I High molecular weight
based upon a unique phase-
genomic DNA suitable
for many applications
partitioning technology in combination
with the selective binding of the
genomic Dna to a special axyPrep
column. The purified genomic Dna is
≥30 kb in size, and is particularly well
suited for Pcr, Southern blotting and
genomic dNa purified from anticoagulated human blood
samples with the axyPrep Blood genomic dNa Midiprep
Kit. 0.4 µg/lane.
Lanes 1, 3, 5
Unrestricted
Lanes 2, 4, 6
Restricted with EcoR I
M
Axygen 1Kb DNA Ladder
oVerVieW
other applications requiring highly
Midi
purified, high molecular weight
Maxi
release of dNa
genomic Dna. a vacuum manifold and
vacuum source are required.
Phase partition
filtration
Binding
Washing
"Phase-partitioning" is a process covered by
US patent No.7,355,038 and owned by
Axygen Inc.
BLOOD GENOMIC
DNA MIDIPREP
10
axygenBio.com
genomic dNa purified from anticoagulated human blood
(multiple donors) with the axyPrep Blood genomic dNa
Maxiprep Kit. 0.4 µg/lane.
KIT SIZES
2 preps
10 preps
25 preps
CATALOG NO.
aP-Md-Bl-gdNa-2
aP-Md-Bl-gdNa-10
aP-Md-Bl-gdNa-25
BLOOD GENOMIC
DNA MAXIPREP
elution
KIT SIZES
2 preps
10 preps
25 preps
CATALOG NO.
aP-MX-Bl-gdNa-2
aP-MX-Bl-gdNa-10
aP-MX-Bl-gdNa-25
axyPrep™ 96 Blood Genomic DNAkit
high-ThroughPuT
PurificaTion of genomic
Dna from WhoLe BLooD
in a 96-WeLL formaT.
1
2
3
4
5
6
7
8
9
I Protocols for fresh or
10
frozen anticoagulated
blood samples,
lymphocytes and
cultured cells in a 96-well
format
This kit is based upon the efficient
I Up to 10 µg of highly
release of genomic Dna from anticoagulated whole blood or cells by a
special cell lysis and protein-removal
Lanes 1, 3, 5, 7, 9 Blood genomic DNA purified from human blood with
the AxyPrep-96 Blood Genomic DNA Kit.
Lanes 2, 4, 6, 8, 10 Genomic DNAs digested by EcoR I.
0.4 µg Genomic DNA loaded in every lane. Run on a 0.8% agarose gel.
buffer in combination with a Proteinase k
1
digestion.
2
3
4
5
6
7
8
9
10
I Rapid spin protocol
I High molecular weight
genomic DNA product
(≥30 Kb)
The genomic Dna is then selectively
I No alcohol precipitation
adsorbed onto a special 96-well plate for
I Suitable for many
further purification and desalting. yields
of up to 10 µg/well are achievable*. The
purified Dna is predominantly ≥30 kb
purified genomic DNA
per well
Lanes 2-9: GAPDH amplicons generated from 8 human blood genomic DNA
samples. Template DNA samples were randomly selected from 96 genomic
DNA samples purified with the AxyPrep-96 Blood Genomic DNA Miniprep Kit.
downstream applications
in length, and is suitable for a variety of
applications demanding highly purified,
oVerVieW
high molecular weight genomic Dna,
such as Southern blot analysis, Pcr, etc.
sample
lysis
*Yields of approximately 8-10 µg per well
are achievable when processing 200 µl of
human whole blood with elevated leukocyte
levels, non-human blood, lymphocytes and
cultured cells. Routine yields of
approximately 4-5 µg per well are
anticipated when processing 200 µl of
normal human blood.
Binding
Washing
96 blood genomic dNa samples purified from human
blood (200 µl/prep) with the axyPrep-96 Blood genomic
dNa Kit. approximately 0.3 µg genomic dNa/lane on a
0.8% agarose gel.
M:
96 BLOOD
GENOMIC DNA
KIT SIZES
1x96 preps
4x96 preps
12x96 preps
elution
Axygen 1 Kb DNA Ladder
CATALOG NO.
aP-96-Bl-gdNa-1
aP-96-Bl-gdNa-4
aP-96-Bl-gdNa-12
11
axyPrep™ Bacterial
Genomic
DNA
miniPreP kiT
raPiD
iSoLaTion of uP To 20 µg
of genomic Dna from
1mL of BacTeria SamPLe
(~10 x 109 BacTeriaL ceLLS).
M
This kit is suitable for the
1
2
3
4
M
I High yield of up to 20 µg
of highly purified gram/gram+ bacterial
genomic DNA per prep
I High molecular weight
genomic DNA product
(≥30 Kb)
following lysis, separation of the
I Rapid spin and
genomic Dna from proteins,
vacuum protocols
polysaccharides and lipids is achieved
I No alcohol precipitation
by a unique phase-partitioning step.
I Suitable for many
The genomic Dna in the lower phase
is then selectively bound to a special
downstream
applications
axyPrep column for further purification
and desalting. The highly purified
genomic Dna is suitable for a variety of
oVerVieW
applications demanding highly purified,
high molecular weight genomic Dna,
such as Southern blotting, Pcr, etc.
genomic dNa purified from different 1 ml e. coli JM109 cultures with the
axyPrep Bacterial genomic dNa Miniprep Kit. 0.3 µg/lane.
M
lysis
Axygen 1Kb DNA Ladder
Phase
partition
filtration
spin
Binding
Washing
elution
"Phase-partitioning" is a process covered by
US patent No.7,355,038 and owned by
Axygen Inc.
BACTERIAL
GENOMIC DNA
12
axygenBio.com
KIT SIZES
4 preps
50 preps
250 preps
CATALOG NO.
aP-MN-Bt-gdNa-4
aP-MN-Bt-gdNa-50
aP-MN-Bt-gdNa-250
Vacuum
axyPrep™ Body
FluidViral
DNA/RNA
miniPreP kiT
The axyPrep viral Dna/rna miniprep
M
1
2
3
4
5
6
7
8
M
I Plasma, serum, ascites,
csf, urine, etc.
kit provides a raPiD
anD
highLy reProDuciBLe
meThoD To Purify viraL
nucLeic aciD from 250 µl
of BoDy fLuiD, including
I Rapid spin protocol
I No alcohol
precipitation
I Suitable for
plasma, serum, ascites, cerebrospinal
PCR/RT-PCR analyses
fluid, urine, cell culture supernatant,
etc. During lysis, proteins and Pcr
inhibitors are removed by denaturation
and precipitation. viral Dna and rna
remain soluble in the supernatant and
are copurified by binding to a special
axyPrep column. after brief washes to
remove residual impurities and salt, the
purified viral nucleic acid is eluted and
Lane 1
1:10 dilution
Lane 2
1:102 dilution
Lane 3
1:103 dilution
Lane 4
1:104 dilution
Lane 5
1:105 dilution
Lane 6
1:106 dilution
Lane 7
Positive control
Lane 8
Negative control
M
Mixed DNA marker fragments
(5 Kb, 1 Kb, 500 bp and 100 bp)
120 bp viral amplicon
generated by rt-Pcr from
a dilution series of
hepatitis a (106 phage/ml)
viral rNa purified with the
axyPrep Body fluid Viral
dNa/rNa Miniprep Kit.
oVerVieW
can be used immediately.
lysis
The viral nucleic acid purified by this
method is free from contaminants and
Pcr inhibitors, and is particularly
Protein
denaturation
suitable for demanding Pcr/rT-Pcr
analyses.
Protein
precipitation
Binding
Washing
elution
BODY FLUID
VIRAL DNA/RNA
KIT SIZES
4 preps
50 preps
250 preps
CATALOG NO.
aP-MN-Bf-VNa-4
aP-MN-Bf-VNa-50
aP-MN-Bf-VNa-250
13
axyPrep™ PCR Clean-upkit
The axyPrep Pcr cleanup kit employs
1
2
3
4
5
6
7
I Rapid spin and
8
vacuum protocols
a special Binding Solution in
I Efficient purification of
combination with an axyPrep spin
75 bp-10 Kb amplicons
column to achieve high selectivity and
recovery of Dna fragments. This
I Up to 90% recovery
product is designed to Purify
I Removal of
Dna fragmenTS >5 nt/bp
from Pcrs anD oTher
enzymaTic reacTionS,
WiTh an exPecTeD
recovery of aPProximaTeLy
0-90%*. it is not necessary to
remove mineral oil overlays from the
unincorporated primers
<50 nt
I Maintains integrity of
DNA fragments
analysis of Pcr products before ( 1, 3, 5, 7 ) and after ( 2, 4, 6, 8 )
purification with the axyPrep Pcr clean-up Kit.
Lane 1, 2:
5 Kb PCR product
Lane 5, 6:
500 bp PCR product
Lane 3, 4:
1 Kb PCR product
Lane 7, 8:
100 bp PCR product
4
5
I No alcohol precipitation
I Purified DNA fragments
are suitable for many
downstream
applications
Run on a 1.6% agarose gel.
Pcrs before purification. This kit will
remove unincorporated primers (<50
nt), enzymes and unlabeled and
fluorescent dye-labeled mono-
1
2
3
6
7
8
nucleosides. The purified Dna
oVerVieW
fragments are suitable for a variety of
applications, such as sequencing,
sample
ligation, restriction analysis, in vitro
transcription, etc.
analysis of Pcr products before (100%) and after (1-8) purification with
the axygen Pcr clean-up Kit. 50% as 50% recovery, and 100% as 100%
recovery. each lane contains mixed 5 Kb, 750 bp, 500 bp, and 100 bp
dNa fragments. run on a 1.6% agarose gel.
spin
Binding
Washing
*Factors such as fragment length can
influence the percent recovery of amplicons.
PCR CLEAN-UP
1
axygenBio.com
elution
KIT SIZES
4 preps
50 preps
250 preps
CATALOG NO.
aP-Pcr-4
aP-Pcr-50
aP-Pcr-250
Vacuum
axyPrep™ 96 PCR Clean-upkit
high-throughput purification of Pcr
50%
1
2
3
4
5
6
7
8
100%
I High-throughput,
96-well plate format
amplicons in a 96-well format. The
I Efficient purification of
axyPrep-96 Pcr clean- up kit employs
75bp-10kb amplicons
a special Binding Solution in
combination with an optimized 96-
I Up to 90% recovery
well plate to Purify
I Rapid spin and vacuum
SingLeanD DouBLe-STranDeD
Dna fragmenTS >5 nt/bp
from Pcrs anD oTher
enzymaTic reacTionS. it is
protocols
I No alcohol
precipitation
I Purified amplicons are
not necessary to remove mineral oil
suitable for many
downstream
applications
overlays from the Pcrs before
purification. This protocol will remove
unincorporated primers (<50 nt),
enzymes and unlabeled and
fluorescent dye-labeled mononucleosides. The purified Dna
fragments are suitable for a variety of
applications, such as cloning,
sequencing, igation, restriction
analysis of Pcr products before (100%) and after (lanes 1-8) purification
with the axyPrep Pcr clean-up Kit. 50% = 50% Pcr product recovery,
and 100% = 100% Pcr product recovery. each lane contains mixed 5 Kb,
750 bp, 500 bp, and 100 bp dNa fragments. run on a 1.6% agarose gel.
oVerVieW
analysis, in vitro transcription, etc.
sample
Binding
Washing
elution
96 PCR CLEAN-UP
KIT SIZES
1x96 prep
4x96 preps
24x96 preps
CATALOG NO.
aP-96-Pcr-1
aP-96-Pcr-4
aP-96-Pcr-24
15
axyPrep™ DNA Gel Extractionkit
The axyPrep Dna gel extraction kit
1
2
3
4
5
6
7
I Efficient purification of
and Binding buffers in combination
75 bp-10 Kb*
DNA fragments
with a convenient spin column to
Purify Dna fragmenTS
from eiTher Tae or TBe
agaroSe geLS (reguLar
anD LoW-meLT). Dna fragments
I Typical yields of up
to 85%*
I Maintains chemical
in a size range of 5 bp up to 10 kb*
analysis of Pcr product recovery after purification with the axyPrep
dNa gel extraction Kit
Depending upon the length of the
Dna fragment, the recovery rate is
Lane 1
Lane 2
5 Kb DNA fragment, no extraction
5 Kb DNA fragment after extraction
approximately 60-5%. unique colored
Lane 3
Lane 4
1 Kb DNA fragment, no extraction
1 Kb DNA fragment after extraction
buffer formulations ensures complete
Lane 5
Lane 6
500 bp DNA fragment, no extraction
500 bp DNA fragment after extraction
Lane 7
Lane 8
100 bp DNA fragment, no extraction
100 bp DNA fragment after extraction
gel solubilization while also protecting
the Dna fragments against damage
I Rapid spin and
vacuum protocols
employs optimized gel Solubilization
can be efficiently recovered.
8
integrity of DNA
fragments
I No alcohol
precipitation
I Suitable for many
downstream
applications
oVerVieW
and degradation. Dna fragments
gel containing dNa
purified by this method are full-length
with high biological activity. These
solubilization
fragments are suitable for all routine
molecular biology applications, such as
ligation, Pcr, sequencing, etc.
Binding
Washing
*Factors such as fragment length and gel
composition can influence the percent
recovery of DNA fragments.
DNA GEL
EXTRACTION
16
axygenBio.com
elution
KIT SIZES
4 preps
50 preps
250 preps
CATALOG NO.
aP-gX-4
aP-gX-50
aP-gX-250
axyPrep™ Multisource RNA
miniPreP kiT
The axyPrep multisource Total rna
M
miniprep kit is designed to
I For animal tissues,
Mouse liver
1
2
20 mg of fresh mouse liver was used
for each prep. tissue was flash frozen
with liquid nitrogen and disrupted
using a mortar and pestle. each sample
was eluted with 100 µl te. 2 µl of each
sample was loaded on gel.
ProDuce uP To 0 µg of
highLy PurifieD ToTaL
ceLLuLar rna from a
WiDe array of DifferenT
STarTing maTeriaL TyPeS,
protocols
or proteinase digestion
I No column clogging
organic extractions. a special
I Up to 80 µg of highly
precipitation step removes cellular
opportunity for column clogging and
I Rapid spin and vacuum
I No organic extraction
without the use of proteinase or
debris and most gDna, eliminating the
plant tissues, cultured
cells, bacteria, yeast
and filamentous fungi
yeast
M
1
2
increasing both rna binding capacity
(yield) and purity. The rna is bound to
a mini spin/vac column for washing
50 mg of yeast paste was used for
each prep. samples were flash frozen
with liquid nitrogen and disrupted
with mortar and pestle. each sample
was eluted with 100 µl te. 2 µl of
each sample was loaded on gel.
intact, highly purified
total cellular RNA
oVerVieW
and desalting, using vacuum. The rna
produced by this kit is exceptionally
lysis
pure and highly intact and is suitable
for the most demanding applications.*
dNa/Protein
Precipitation
centrifugation
corn leaves
M
1
2
50 mg of Corn leaves was used for
each prep. samples were flash frozen
with liquid nitrogen and disrupted
using a mortar and pestle. Purified rNa
was eluted with 100 µl te and 4 µl
loaded per gel.
spin
Vacuum
Binding
Washing
*Applications which are sensitive to small
amounts of residual gDNA may require
additional steps to digest and remove these
traces of gDNA.
MULTISOURCE
RNA MINIPREP
elution
KIT SIZES
CATALOG NO.
4 preps
aP-MN-Ms-rNa-4
50 preps
aP-MN-Ms-rNa-50
250 preps
aP-MN-Ms-rNa-250†
†
Shipped as 5 x AP-MN-MS-RNA-50
1
axyPrep™ Multisource
RNA
miDi & maxiPreP kiTS
The axyPrep multisource Total rna
M
rat liver
1
2
rat Brain spinage root corn leaves
3
4
5
6
7
8
9
yeast
10
midiprep and maxiprep kits are
designed to produce uP
To 1 mg
(miDi) anD 2 mg (maxi) of
highLy PurifieD ToTaL
ceLLuLar rna from a
WiDe array of DifferenT
STarTing maTeriaL TyPeS,
protocols
I No organic extraction
precipitation step removes cellular
debris and most gDna, eliminating the
Purified rNa eluted in 300 µl te Buffer. 1 µg loaded per lane.
organic extractions. a special
plant tissues, cultured
cells, bacteria, yeast
and filamentous fungi
I Rapid spin and vacuum
rNa purified with the axyPrep Multisource total rNa Midiprep Kit
M:
axygen 100bp dNa ladder
lanes 1, 2: 200 mg rat liver (duplicate samples)
lanes 3, 4: 300 mg rat brain
lanes 5, 6: 500 mg spinach root
lanes 7, 8: 500 mg corn leaves
lanes 9, 10: 2x108 S. cerevisae
without the use of proteinase or
I For animal tissues,
or proteinase digestion
I No column clogging
I Up to 1 mg and 2 mg
respectively of highly
purified total RNA
opportunity for column clogging and
increasing both rna binding capacity
(yield) and purity. The rna is bound to
M
rat liver
1
2
rat Kidney
3
4
holly leaves
5
6
Bacteria
7
8
a midi column for washing and
desalting, using either centrifugation
oVerVieW
Midi
Maxi
lysis
or vacuum. The rna produced by this
product is exceptionally pure and
Precipitation
highly intact and is suitable for the
most demanding applications.* a
vacuum manifold and vacuum source
are required for the midiprep kit.
rNa purified with the axyPrep Multisource total rNa Maxiprep Kit
M:
axygen 100bp dNa ladder
lanes 1, 2: 800 mg rat liver (duplicate samples)
lanes 3, 4: 800 mg rat kidney
lanes 5, 6: 1.3 g holly leaves
lanes 7, 8: 4x1010 E. coli
dNa/protein
removal
Purified rNa eluted in 2 ml te Buffer. 1 µg loaded per lane.
Binding
Washing
*Applications which are sensitive to small
amounts of residual gDNA may require
additional steps to digest and remove these
traces of gDNA.
MULTISOURCE
RNA MIDI &
MAXIPREP
18
axygenBio.com
elution
KIT SIZES
2 preps
10 preps
25 preps
CATALOG NO.
aP-Md-Ms-rNa-2
aP-Md-Ms-rNa-10
aP-Md-Ms-rNa-25
axyPrep™ Blood miniPreP
TotalRNA
kiT
The axyPrep Blood Total rna miniprep
kit will produce approximately 3-5 µg
of highLy PurifieD ToTaL
ceLLuLar rna from 00 µl
WhoLe BLooD SamPLeS,
total rNa was prepared from
200 µl of each sample type.
Porcine blood
1
3
Purified rNa was eluted in
60 µl te and 8 µl of sample
was loaded per lane.
without the use of proteinase or harsh
Lanes 1, 3
organic extractions. a special
Lanes 7, 8
Porcine blood Axygen
Blood RNA Mini Kit
Mouse blood Axygen
Blood RNA Mini Kit
precipitation step removes cellular
Mouse blood
7
8
I Rapid spin and vacuum
protocols
I High yield of up 5 µg
from 400 µl of whole
blood
I No organic extraction
or proteinase digestion
I No column clogging
I Highly intact, highly
debris and most gDna, eliminating the
purified total cellular
RNA
opportunity for column clogging and
increasing both rna binding capacity
(yield) and purity. The rna is bound to
a mini spin/vac column for washing
and desalting, using either
oVerVieW
centrifugation or vacuum. The rna
produced by this product is
erythrocyte lysis
exceptionally pure and highly intact
and is suitable for the most
demanding applications.*
leukocyte lysis
dNa/Protein
Precipitation
centrifugation
spin
Vacuum
Binding
*Applications which are sensitive to small
amounts of residual gDNA may require
additional steps to digest and remove these
traces of gDNA.
BLOOD TOTAL
RNA MINI
Washing
elution
KIT SIZES
CATALOG NO.
4 preps
aP-MN-Bl-rNa-4
50 preps
aP-MN-Bl-rNa-50
250 preps
aP-MN-Bl-rNa-250†
†
Shipped as 5 x AP-MN-BL-RNA-50
19
axyPrep™ Micro (miRNA) RNA
The axyPrep mirna miniprep kit
rePreSenTS a neW
aPProach for ceLLuLar
“micro” rna (mirna)
PurificaTion. it produces a
I For animal tissues,
tomato leaf, 30 mg per lane
1
2
3
4
5
plant tissues and
cultured cells
I Enriches for miRNAs
and siRNAs
purified cellular rna subfraction which
I Rapid spin protocol
is highly enriched for lower molecular
I No organic extraction
or proteinase digestion
weight species, such as mirna and
I No column clogging
sirnas. This kit is designed to eliminate
the problems associated with other
spin column-type rna kits, such as
clogged columns and incomplete
purification. Proteins and genomic
Dna are precipitated and removed
from the cell lysate. Then, larger rnas,
Northern Blot depicting Micro rNa (mirNa)
fractions isolated from 30 mg tomato leaf
samples by different methods. the target is
a 22 nt mirNa, Mr138
lanes 1, 2:
lanes 3, 4:
such as mrna, 1s and 2s rrna and
residual Dna are removed by binding
lanes 5:
axyPrep microrNa
Miniprep Kit
guanidinium - phenol chloroform extraction
control
oVerVieW
lysis
dNa/Protein
Precipitation
to a spin/vac column. mirna and
sirna remain unbound and are
recovered by the addition of
isopropanol and centrifugation.
remove
large rNa
elute micro
rNa fraction
centrifugation
desalting
resuspension
MICRO RNA
20
axygenBio.com
KIT SIZES
CATALOG NO.
4 preps
aP-MN-mirNa-4
50 preps
aP-MN-mirNa-50
250 preps
aP-MN-mirNa-250†
†
Shipped as 5 x AP-MN-miRNA-50
axyvacVacuumManifold
I Single manifold for
The axyvac vacuum manifold
BOTH column- and
plate-based sample
preps
System provides a
comPLeTe
SoLuTion for fiLTraTionTyPe SamPLe PurificaTion.
I Integrated controls for
it is designed to accommodate a
vacuum regulation
wide variety of different columns
I Constructed of durable,
and SBS-format, 96- and 3-well
long-lasting materials
plates. The axyvac is constructed
from durable, easy-to-clean
materials. The manifold base is
injection molded from aBS plastic.
The manifold top and middle
support are molded from
polycarbonate. The 96-well plate
support is constructed from stainless
steel. These materials offer
The axyvac vacuum manifold
exceptional resistance against
comes complete with all
components* required to
breakage and chemical degradation.
perform sample preparation with
The high-clarity polycarbonate
both columns and 96- or 3-
components allow progression of
well plates. components
the various filtration processes to be
provided:
monitored visually.
• manifold base
• manifold top
• middle support
• Plate support
• Block support
• Waste reservoir
*
The customer must supply tubing to
connect the vacuum manifold to the
axyVac with 96-well filter plates
AXYVAC
vacuum source.
DESCRIPTION
CATALOG NO.
Vacuum Manifold kit aP-VM
21
axygenDNA Markers
I Five markers, 100 bp ladder, 1 kb
ladder, Low Range, High Range
and Broad Range. Allow sizing of
a wide range of DNA fragments
(100 bp to 10000 bp)
I Stable at Ambient Temperature
I Long Shelf life of 2 years at
ambient temperature
I Crisp and precise band patterns
I Ready-To-Use. Formulated with
Bromophenol Blue for easy
loading
of Dna
markerS To Serve aS STanDarDS
for WiDe range of aPPLicaTionS.
of 600 µl and is sufficient for up to 125 lanes.
markers are pre-loaded with Bromophenol Blue
our 100 bp and 1 kb ladders represent the 2
dye and are supplied ready-for-use.
most popular markers used for Dna fragment
all five markers create precise sharp bands with
all markers are stable at ambient temperature
size determination and allow sizing from 100 bp
locations that are consistent under conventional
and have a long shelf of up to two years. This
to 10000 bp. These markers are supplied at a
electrophoresis conditions.
allows you to store the markers on your work
concentration of 50 µg/500 µl (1 µg/10 µl).
Sharp bands allow precise detection by gel
bench for immediate access, saves room in your
The Low, high and Broad range markers were
imaging systems and software for accurate
refrigerator, and reduces shipping costs.
introduced recently to offer an alternative to
molecular weight calculations.
each vial of the 100 bp and 1 kb ladders has 500 µl
expensive brands and offer the widest sizing range
The markers are robust and can be used for fast
of the marker sufficient for up to 100 lanes. The
from 125 bp to 10000 bp. These three markers
electrophoresis runs (10-15 min).
low high and broad range markers come in vials
are supplied at a concentration of 1 µg/ 10 µl.
axygen offers a range
DNA MARKER
22
axygenBio.com
LADDER
100 bp, 100-3,000 bp
1 kb, 300-10,000 bp
Broad range, 250 bp-8,000 bp
high range, 1,000 bp-10,000 bp
low range, 125 bp-3,000 bp
CATALOG NO.
M-dNa-100bp
M-dNa-1Kb
M-dNa-Br
M-dNa-hr
M-dNa-lr
axygenDNA Markers
100 bp Ladder
DNA Marker
(100-3,000 bp)
the 100 bp dNa ladder
contains 11 discrete dNa
fragments ranging in size
from 100 bp to 3,000 bp.
this marker is ideal for
the size determination of
Pcr products.
bp
3,000
108
1 kb Ladder
DNA Marker
2,000
147
(300-10,000 bp)
1,500
116
1,000
77
800
700
600
500
400
62
54
46
150
60
300
56
200
60
100
Broad Range
Marker
(250-8,000 bp)
the Broad range marker
has 11 discrete fragments:
75 ng/10 µl of 8000 bp,
75 ng/10 µl of 6000 bp,
75 ng/10 µl of 5000 bp,
65 ng/10 µl of 4000 bp,
90 ng/10 µl of 3000 bp,
165 ng/10 µl of 2000 bp,
60 ng/10 µl of 1500 bp,
210 ng/10 µl of 1000 bp,
50 ng/10 µl of 750 bp,
60 ng/10 µl of 500 bp,
75 ng/10 µl of 250 bp
Low Range
Marker
ng/10 µl
the 1 kb dNa ladder
contains 13 discrete dNa
fragments ranging in size
from 300 bp to 10,000 bp.
this marker is ideal for the
size determination of
digested dNa.
bp
ng/10 µl
SPECIFICATIONS :
10,000
8,000
6,000
5,000
4,000
80
80
80
80
80
• Each Vial has 500 µl of marker
3,000
2,500
120
123
• Concentration is 1 µg/10 µl
2,000
81
1,500
73
1,000
41
700
57
500
61
• Ready-to-use. Contains
300
44
Bromophenol Blue Dye
bp
ng/10 µl
60
bp
ng/10 µl
8,000
6,000
5,000
4,000
3,000
2,000
75
75
75
65
90
165
1,500
60
1,000
750
210
50
500
60
250
75
bp
ng/10 µl
3,000
2,000
1,500
120
220
65
High Range
Marker
• Recommended Loading:
5-10 µl per lane
• Each vial is sufficient for 100
lanes
LOw RANGE, HIGH RANGE
AND BROAD RANGE LADDERS
• Each Vial has 600 µl of marker
(1,000-10,000 bp)
the high range marker
has 8 discrete fragments:
100 ng/10 µl of 10000 bp,
100 ng/10 µl of 8000 bp,
100 ng/10 µl of 6000 bp,
100 ng/10 µl of 5000 bp,
100 ng/10 µl of 4000 bp,
230 ng/10 µl of 3000 bp,
70 ng/10 µl of 2000 bp and
200 ng/10 µl of 1000 bp.
100 BP AND 1 KB LADDERS
10,000
8,000
6,000
5,000
4,000
100
100
100
100
100
3,000
230
2,000
70
1,000
200
• Concentration is 1µg/10 µl
• Recommended Loading:
5-10 µl per lane
• Each vial is sufficient for 125
lanes
• Ready-to-use. Contains
Bromophenol Blue Dye
(125-3,000 bp)
the low range marker
has 9 discrete fragments:
120 ng/10 µl of 3000 bp,
220 ng/10 µl of 2000 bp,
65 ng/10 µl of 1500 bp,
235 ng of 1000 bp,
70 ng/10 µl of 750 bp,
80 ng/10 µl of 500 bp,
105 ng/10 µl of 250 bp, and
105 ng/10 µl of 125 bp.
1,000
235
750
70
500
80
250
105
125
105
Quality Control: each lot is tested under
appropriate electrophoresis conditions. The
Dna concentration is determined
spectrophotometrically and the absence of
nucleases is confirmed by appropriate
tests. Temperature stability is confirmed by
burn-in tests where the Dna marker is
subject to storage at 65°c and 95%
humidity for 2 weeks.
Storage and Shelf-life
ambient conditions (~22°c) for 2
years. Warmer conditions require
refrigeration.
23
AxygenProtein Markers
I Low range, Mid range and
figure: coomassie
and silver stain
Protein Markers run
at 100V on a 4-12%
gradient
polyacrylamide gel.
reaDy To uSe, SingLe STeP
DirecT STaining of
ProTeinS in
PoLyacryLamiDe geLS
Broad range protein
markers allow fast sizing
of a wide range of proteins
(2.5 kD to 205 kD)
I 2 distinct marker sets
The nrL ultra fast coomassie Staining
optimized for either
Coomassie or Silver
staining
kit is based on a colloidal coomassie
stain where only protein bands get
stained leaving the background gel
I Highly purified
clear. The staining is optimized for
electrophoresis grade
proteins ensure consistent
migration patterns under
conventional
electrophoresis conditions
more sensitivity than regular
coomassie stains, and protein bands
can be visualized within 5-10 minutes
during the staining procedure.
I Sharp bands for precise
Low Range Protein Marker
Broad Range Protein Marker
the low range marker has 7
discrete fragments:
the Broad range marker has 12
discrete fragments:
1 Myosin
205,000 kd
2 Beta-galacatosidase 116,000 kd
3 Phosphorylase-b
97,400 kd
4 albumin Bovine
66,000 kd
5 albumin egg
45,000 kd
6 glyceraldehyde-3-Pdehydrogenase
36,000 kd
7 carbonic anhydrase 29,000 kd
8 trypsin inhibitor
20,100 kd
9 lysozyme
14,300 kd
10 aproteinin
6,000 kd
11 insulin hc
3,500 kd
12 insulin lc
2,500 kd
1 glyceraldehyde-3-Pdehydrogenase
36,000 kd
Loading Volume:
2 carbonic anhydrase
29,000 kd
5 µl
.5 µl
10 µl
15 µl
3 trypsin inhibitor
20,100 kd
4 lysozyme
14,300 kd
5 aproteinin
6,000 kd
6 insulin hc
2,500 kd
(for 1.0 mm thick mini gel)
(for 1.5 mm thick mini gel)
(for 1.0 mm thick regular gel)
(for 1.5 mm thick regular gel)
Mid Range Protein Marker
Directions:
• Place marker tube in a 3°c water bath
before loading
• markers are ready-to-use; load directly
from the vial
• markers volume may need to be
adjusted depending on gel thickness and
staining methods
COOMASSIE
PROTEIN MARKER
24
axygenBio.com
software detection and
molecular weight
determination
the Mid range marker has 6
discrete fragments –
1 albumin Bovine
66,000 kd
2 albumin egg
45,000 kd
3 glyceraldehyde-3-Pdehydrogenase
36,000 kd
4 carbonic anhydrase
29,000 kd
5 trypsin inhibitor
20,100 kd
6 lysozyme
14,300 kd
PRODUCT DESCRIPTION
low range 2.5kd-36kd
Mid range 14.3kd-66kd
Broad range 2.5kd-205kd
QTY
500 µl
500 µl
500 µl
CATALOG NO.
MN-Pro-c-lr
MN-Pro-c-Mr
MN-Pro-c-Br
I Ready-to-use. Formulated
with Bromophenol Blue dye
SPECIFICATIONS :
each vial has 500 µl of
solution for 100 x 5µl
applications
Buffer contains: sds,
glycerol, Bromophenol Blue,
tris-hcl
Storage and Shelf Life
store at -20˚c. Markers are stable for six
months at -20˚c
SILVERSTAIN
PROTEIN MARKER
PRODUCT DESCRIPTION
low range 2.5kd-36kd
Mid range 14.3kd-66kd
high range 2.5kd-205kd
QTY
500 µl
500 µl
500 µl
CATALOG NO.
MN-Pro-s-lr
MN-Pro-s-Mr
MN-Pro-s-Br
AxygenX-Gal-IPTG Spray
I Easy, accurate and
x-gal-iPTg spray is a convenient ready-
convenient
to-use SoLuTion
of
oPTimaL quanTiTieS of
iPTg, x-gaL anD
STaBiLizerS presented in a spray
I Sprays deliver optimal
concentration and
volume consistently for
consistent and accurate
results
bottle for easy, accurate and consistent
I No need to weigh X-Gal
screening of white and blue colonies.
and IPTG and mixing
them in special
solutions saving time
and cost
I Proprietary mixtures of
stabilizers ensure the
IPTG and X-Gal are
stable for 6 months
when stored under
recommended
x-gal-iPTg spray is a ready-to-use solution
that can be directly sprayed on agar plates
containing appropriate antibiotics.
Spray solution in provided a brown bottle
and it is sufficient for approximately 100
plates (100 mm size).
hold the bottle a few inches from the plate
and spray 3- times (~200 µl - equivalent
to about 1.3 mg of each of x-gal and iPTg
per plate).
absorbs the solution quickly, it may be
necessary to use spreader.
Dry the plate by keeping it opened in a
hood after spray.
APPLICATIONS :
colony screening for
recombinant plasmids
no visible solution should be visible
once plates are dried. if plates are freshly
made, it may take about 15 minutes to
dry the plates. otherwise, it may less
than 5 minutes. after spraying, agar
plates could be stored at °c for later use.
note: use hood, safety glasses and gloves
while using spray solution.
Let the plates dry after spray. Spreader is
not necessary, but if plate are too dry and
X-GAL/
IPTG SPRAY
QTY
20 ml
CATALOG NO.
reg-XgiP-l-1
Storage: 4°c. shipped at ambient.
Stability: 6 months when stored at 4°c.
25
™
axygenSmart ampicillin
I Smart Ampicillin is
Smart ampicillin™ is a SPeciaLLy
optimized for
expression studies
ensuring perfect
recombinant colonies
formuLaTeD amPiciLLin
anTiBioTic that is optimized for
expression studies. Smart ampicillin is
I Smart Ampicillin is
superior to common antibiotics like
stronger than
traditional antibiotics
ampicillin, carbenicillin and methicillin.
These regular antibiotics are degraded
I No appearance of
by enzymes secreted by bacteria over
satellite colonies after
transformation
time and the result is the undesirable
appearance of satellite or non-
I Better suited for
recombinant colonies. Smart ampicillin
Results
is specially formulated to overcome
a. satellite colony growth in plates after 12 hours in
media containing regular ampicillin antibiotic.
this bacterial enzyme degradation and
downstream analysis
such as colony
counting, picking, etc
I Convenient pre-made
hence present you with Satellite free
solutions of 1ml at
1,000x concentrate
recombinant colonies for an extended
period of time.
I Suitable for liquid
cultures or plates
APPLICATIONS :
Perfect replacement for
B. smart ampicillin plates after 24 hours of incubation.
traditional antibiotics
such as ampicillin,
carbenicillin and
Methicillin to screen
recombinants in cloning
and expression using
bacterial plasmids
as a strong antibiotic in
tissue cultures
SMART
AMPICILLIN 1000x
26
axygenBio.com
QTY
1 ml
CATALOG NO.
reg-saMP-l-1
axygenTaq Polymerase
Taq PoLymeraSe kiT from
axygen BioScience iS
SuPPLieD comPLeTe WiTh 10x
STanDarD Taq Buffer with
1
2
3
4
5
6
7
8
I Highly pure Taq
polymerase from a
recombinant source
I High activity for standard
magnesium, buffer without magnesium
PCR applications; gives
high yields
and magnesium chloride so it is can be
I Supplied with 10X buffer
used for a range of applications. Taq is
that has optimum levels
of Mg++
supplied at a concentration of 5 units/µl.
I Exceptional value
master mixes and dnTPs are also available.
Reagents Supplied
• Standard Taq Polymerase - 500 units
• Taq reaction Buffer - 1 ml
• Taq reaction Buffer-mg2 free - 1 ml
• magnesium chloride - 1 ml
Source
an E. coli strain that carries the Taq Dna
Polymerase gene from Thermus aquaticus.
Unit Definition
one unit is defined as the amount of
enzyme that will incorporate 10 nmol
of dnTP into acid-insoluble material in
30 minutes at 5°c.
compared to high price
brands
axygen taq – Proven quality: 756 bp Pcr fragment was
generated by using 50 ng of puc vector (template dNa) and 5
units of axygen taQ. Primers concentration, dNtP and buffer
were used as specified by different vendors.
from 100 µl of Pcr reaction mixtues, 10 µl of reaction mixture
was loaded in a gel to visualize the quality of Pcr fragment.
lane 1. standard dNa ladder
lane 5. company Q
lane 2. company P
lane 6. company c
lane 3. company N
lane 7. axygen taq
lane 4. company B
lane 8. standard dNa ladder
APPLICATIONS :
Polymerase chain
reaction (Pcr)
high-throughput Pcr
dhPlc
. 2-50 pg plasmid or 50-500 ng
genomic template
5. 1 x buffer (supplied at 10x
concentration)
6. Denature at 9°c
. extend 1 minute/kb
1. add 1.0 to 2.0 units Taq Dna
polymerase
2. add 200 µm of each dnTP
3. 0.2-0.5 µm each primer
DESCRIPTION
taq Polymerase
taq Polymerase
dNtPset (25 umol/dNtP, 100 mM)
dutP (25 umol, 100 mM)
temperatures for short
periods
Primer extension
PROTOCOL: STANDARD PCR
Taq Dna polymerase is a thermostable Dna polymerase that
possesses a 5´3´ polymerase
activity and a double-strand
specific 5´3´ exonuclease
activity.
I Can be stored at ambient
QTY
500 units
1000 units
100 µl
25 umol
Storage Buffer: 20 mm Tris-hcl (ph .0),
100 mm kcl, 0.5% Tween 20, 0.1 mm eDTa,
1mm DTT, and 50% glycerol.
10x Taq Reaction Buffer: 500mm Tricine
(ph .0), 120mm Potassium acetate, 30 mm
magnesium Sulphate, 0.1% Triton x-100; and
50 µg/ml BSa (Dna and rna free).
10x Taq Reaction Buffer Magnesium Free:
500mm Tricine (Ph .0), 20mm Potassium
acetate, 0.1% Triton x-100; and 50 µg/ml BSa
(Dna and rna free).
CATALOG NO.
Pcr-taQ-r-5
Pcr-taQ-r-10
Pcr-dNtP-s-254
Pcr-dutP-r-25
Microarray analysis
Long Term Storage: -20°c
Shelf Life: 12 months at -20°c
license Notice: the Pcr process is the subject of european
Patent Nos. 201,184 and 200,262 owned by hoffman-laroche
and licensee applied Biosystems. these Pcr patents expired in
March 28, 2006 and the corresponding Pcr process patents in
the united states expired on March 29, 2005. however some
other applications of taq polymerase may require a license. this
depends on the application and it is the sole responsibility of the
buyer to ensure that use of the product does not infringe the
patent rights of third parties.
DESCRIPTION
QTY
CATALOG NO.
dNtP mixture (100 mM total conc. 100 umol/unit Pcr-dNtP-M-100
25 mM each dNtP)
taq Polymerase Master Mix 5X
1 ml/unit
Pcr-taQ-MX-1
27
AxygenAgarose
LE
(LoW eLecTroenDoSmoSiS)
agarose Le is a high
quaLiTy
moLecuLar BioLogy
graDe agaroSe SuiTaBLe
for anaLyTicaL anD
PreParaTive
eLecTroPhoreSiS of
nucLeic aciDS. nucleic acid
separation with agarose Le is between
0.2–15 kbp depending on the
concentration of agarose Le.
nucleic acid fragments separated with
agarose Le can be blotted to nylon or
PROTOCOL: ELECTROPHORESIS of DNA and RNA
The most commonly used technique for Dna separation is electrophoresis
in horizontal agarose gels submerged in either tris-acetate or tris-borate buffer.
rna molecules are separated in denaturing agarose gels containing
formaldehyde. rna gels are submerged in moPS buffer.
APPLICATIONS :
I DNA electrophoresis
I Analysis of PCR
products
step 1: add agarose Le to a measured volume of buffer in a beaker
or flask that is 3 times the volume of solution prepared. The
amount of agarose Le added depends on the desired
concentration.
I Separation of
step 2: Dissolve the agarose by melting, simply by heating the slurry
in a boiling water bath. if you are using a microwave oven,
heat on high power and mix gently.
I Electrophoresis of RNA
step 3: cool the solution to approx. 50°c before pouring. efficient
separation of Dna fragments of a wide size range can be
achieved by adjusting agarose Le concentration (see table).
nitro-cellulose membranes by all
Concentration of
standard blotting techniques.
Agarose LE %
DNA separation
range (kbp)
Size of linear DNA
fragment (bp)
0.8
1 to 15
950
1
5 to 10
525
1.25
0.3 to 5
450
1.5
0.2 to 4
400
1.75
0.2 to 2.5
300
Detection with non-radioactive probes,
e.g. digoxigenin (Dig) - labeled nucleic
acids, does not interfere with the use
of agarose Le.
restriction
endonuclease digests
of DNA
I Protein Electrophoresis
such as radial diffusion
Le is tested for preparative
electrophoresis and isolation of Dna
a
fragments. however, we recommend
B
SPECIFICATIONS :
c
clarity 1.5% (Ntu)
electroendosmosis (eeo)
sulphur as so4
also our low melting point agarose Lm
that is optimized for these applications.
agarose le gels in iX tae buffer
a-0.75%, B-1%, and c-1.25%
Marker: 1kb ladder.
electrophoresis conditions:
submarine gel, 2 hours 30 min,
4.5 V/cm in 1X tae
DESCRIPTION
agarose le
agarose le
agarose le
28
axygenBio.com
QTY
100 gm
500 gm
5 kg
CATALOG NO.
agr-le-100
agr-le-500
agr-lM-5000
≤ 3%
0.05 - 0.13
0.14%
gelling temperature (1.5%) 36°c (±1.5°c)
Melting temperature (1.5%) 88°c (±1.5°c)
gel strength (1%)
1200 g/cm2
gel strength (1.5%)
2500 g/cm2
dNase
None detected
rNase
None detected
AxygenAgarose
LM
and
MS
(LoW meLTing anD moLecuLar Screening)
agarose Lm is a LoW
meLTing
agaroSe ThaT aLLoWS for
The recovery of
unDamageD nucLeic aciDS
a
B
c
a
B
AGAROSE LM :
c
I Highest gelling/melting
temperatures and gel
strength
23,130 bp
I Electrophoresis of DNA
below their denaturation temperature.
1,230 bp
The low gelling temperature ensures that
2,322 bp
the agarose will be in a liquid state at a
517 bp
temperature range where in-gel
manipulations can be performed
154 bp
without prior extraction of the Dna from
Screening agaroSe for
imProveD reSoLuTion of
Dna fragmenTS with 500 bp or
I Suitable for tissue and
cell culture and viral
plaque assays
I Ideal for Preparative
electrophoresis
the gel slice.
agarose mS is a moLecuLar
fragments ≥ 1000 bp
agarose lM at different
concentrations.
a-0.75%, B-1% and c-1.25%.
Marker: 1kb ladder, 0.5 μg/lane.
running conditions: 1X tae
buffer, 4,5V/cm, 2 hours 30 min.
less, especially primer–sized fragments.
AGAROSE MS:
I High resolution of short
a
B
c
d
PCR products and DNA
fragments for improved
clarity of the gel,
enhancing visibility
I Stronger gel structure
SPECIFICATIONS :
LM
MS
Clarity 1.5% (Ntu)
≤7
≤4
Electroendosmosis (Eeo)
≤ 0.12
≤ 0.12
Sulphur as SO4
0.11%
0.12%
Gelling Temperature (1.5 %) ≥31°C
24-28°C
Melting Temperature (1.5 %) ≥76°C
≥65.5° C
Gel Strength (3%)
≥500 G/Cm2 ≥500 G/Cm2
DNase
--- None Detected --RNase
--- None Detected --DNA Resolution ≥1000 bp
Finely
Finely
Resolved
Resolved
DESCRIPTION
agarose lM
agarose lM
agarose Ms
agarose Ms
agarose Ms gel, 3%
concentration in 1X tae buffer.
Markers: lane 1- 250 bp ladder
lane 2
100 bp ladder
lane 3
Molecular weight
marker V (roche)
lane 4
10 bp ladder
electrophoresis conditions:
submarine gel, 2 hours,
4.5 V/cm in 1X tae buffer
QTY
50 gm
100 gm
50 gm
100 gm
and higher gel strength
for better handling. The
chances of gels
breaking or cracking
are greatly minimized,
even at lower
concentrations
I High gel strength
allows use in blotting
I Low DNA binding for
easy recovery
CATALOG NO.
agr-lM-50
agr-lM-100
agr-Ms-50
agr-Ms-100
29
ultra fastProtein Stain
The axygen ultra fast Protein Staining
coLLoiDaL
ProTein STain Where
onLy ProTein BanDS geT
STaineD Leaving The
BackgrounD geL cLear.
kit is based on a
The staining is optimized for more
STEP-BY-STEP STAINING PROCEDURE:
Shake the ultra fast Staining solution bottle well before use.
1
2
sensitivity than regular Protein stains,
and protein bands can be visualized
within 5-10 minutes during the
3
staining procedure. following the
staining process, the protein band
following electrophoresis, wash gels 3 times in de-ionized water to
remove surface SDS in the gel. each wash should be in a large volume
of water at an interval of 5 minutes each. for gels with no SDS (nondenaturing gels), a single wash is sufficient before staining step.
axygen special staining trays are highly recommended for mini gels.
for isoelectric focusing gels, prefixing the gel in 20% Tca (Trichloro
acetic acid) for 30 minutes followed by extensive washing to remove
Tca is recommended before proceeding with ultra fast staining step.
remove all water from the gel tray and add 25-50 ml of ultra fast
Staining solution sufficient amount to cover the gel. Place the gel in a
gentle shaker for 30-60 minutes depending on gel thickness. Protein
bands can be visible within 5 minutes of staining and intensify
maximum within 60 minutes.
intensities may be further enhanced by
equilibration of the stained gel in de-
ionized water. The optimized staining
procedure can detect protein bands in
ng or sub-ng quantities.
rinse the stained gel in 50-100 ml de-ionized water for 2-3 cycles. rinsing
the gel with de-ionized water enhances the band intensity of proteins.
gel can be stored in de-ionized water. alternatively, it can be dried using
axygen gel drying solution for permanent records with no cracking.
Staining of proteins using UltraFast Stain
1
2
3
4
5
6
Myosin
galactosidase
albumin bovine
205 kd
160 kd
66 kd
36 kd
albumin
gPdh
29 kd
carbonic anhydrase
21 kd
trypsin inhibitor
14 kd
lysozyme
45 kd
12% sds gel was run with various proteins at different
concentrations. following electrophoresis, gel was stained with
ultrafast stain for 10 minutes. destained with water for 10 minutes.
results show clear staining of various proteins without background
staining
DESCRIPTION
ultrafast Protein stain
axygen special staining tray
gel drying solution
30
axygenBio.com
QTY
500 ml
1 tray
500 ml
CATALOG NO.
reg-ufPs-500
sdt-1
reg-gds-500
I Unique Ready-To-Use
Protein stain
I Fast-Bands start
appearing in 3 minutes
I Single Step Staining of
proteins in
Polyacrylamide Gels –
saves time
I No de-staining needed –
saves time and money
I Axygen special coated
staining trays minimize
wastage
I Special Gel Drying
solution helps make
permanent record
without cracking
rapidSilver Staining Kit
I Novel temperature
This rapid Silver Staning kit is a
activated staining
system
comPLeTe SySTem for
raPiD anD SenSiTive
STaining of ProTeinS, Dna
anD rna in
PoLyacryLamiDe geLS. The
I Silver Staining of protein
gels in 10 minutes
I Involves an easy 4-step
staining procedure
procedure is simple compared to
I Extremely low
conventional silver staining protocols
background with
reproducible results –
protein detection at high
signal to noise
and takes only 10 minutes to complete.
figure: temperature activated staining using the rapid silver
staining Kit. shows distinct bands after 2 min. of staining.
I Contains reagents for 24
mini gels or 12 regular
size gels
I A special surface
staining tray with lid is
included
APPLICATIONS :
highly sensitive staining of
figure: distinct clear bands
of Protein after staining with
rapid silver staining Kit
proteins, dNa and rNa in
polyacrylamide gels
figure: distinct clear bands of
dNa after staining with rapid
silver staining Kit
Quantification of low
Kit Contents:
Additional Material Required for Staining Procedure
Sufficient for rapid staining of 2 mini gels or 12 standard size gels.
fixing Solutions: 50% methanol + 10% acetic acid
reagent a - reducing Solution in a calibrated dropper bottle 2 ml
mix 500 ml methanol and 100 ml glacial acetic acid and add
reagent B - Silver Solution in a brown wide-mouth bottle 25 ml
water to make 1 litre.
reagent c - Developer Solution in a white plastic bottle 250 ml
rehydration Solution: 5% methanol + % acetic acid
reagent D - Developer enhancer in a calibrated dropper 2 ml
glacial acetic acid: mix 50 ml methanol and 0 ml glacial
one Plastic Staining container (”x ”)
acetic acid and add water to make 1 liter
instructions
abundant proteins in gels
stain both non-denaturing
polyacrylamide gels and
denaturing polyacrylamide
gels containing sds or urea
Visualization of nanogram
quantities of dNa and rNa
Storage and Shelf-life
DESCRIPTION
rapid silver staining Kit
axygen special staining tray
QTY
1 Kit
1 unit
CATALOG NO.
reg-rss-1
sdt-1
store the entire kit at 4°c.
reagent d can be stored at room temperature 25°c.
if crystals form in the reagent-c while storing at 4°c,
warm reagent-c gently until they dissolve.
Shelf life is 12 months if stored at 4°C.
31
axyPrep™ Filtration Plates
This plate has reduced well dimensions
0.3 ml
to accommodate smaller-scale preps,
particularly where elution volumes
I 0.3ml and 1.5ml plates
to suit small volume and
routine applications
must be minimized. it features special
I High clarity
skirted drip directors which eliminate
I Low sample retention
cross-contamination during vacuum-
I Can be nested within
mediated elutions and allow the plate
receiver plates
I SBS-format – compatible
to nest securely within collection
plates and most Pcr plates during
with Robotic systems
centrifugation. The skirted drip
directors also allow the 0.3 ml plates to
nest securely within each other for
direct plate-to-plate transfers using
either vacuum or centrifugation
The 1.5ml filtration Plate is designed to
1.5 ml
function as a routine filtration and
sample preparation plate in molecular
96-well collection Plate, 600 µl
P-DW-500-c
96-well collection Plate, 1.1ml
P-DW-11-c
Packaging = 5 plates/pack, 10 packs/case
biology applications. The 1.5 ml well
volume will accommodate most
biological lysates and provides
sufficient volume for various wash
solutions. Long drip directors allow
direct plate-to-plate transfer of sample
when used in a vacuum format.
* friT maTeriaL conSiSTS of 1.5 mm Thick hyDroPhoBic PoLyeThyLene WiTh a 20 µm Pore Size.
32
axygenBio.com
COLLECTION PLATES
AXYPREP 0.3 ML FILTRATION PLATES
fritted plates
PACK SIZES
50 plates/case
CATALOG NO.
aP-96-0.3f-50
AXYPREP 1.5 ML FILTRATION PLATES
fritted plates
PACK SIZES
50 plates/case
CATALOG NO.
aP-96-1.5f-50
axyPrep™ Deep Well
Plates
anD SeaLing maTS
I Clear Polypropylene 96
reusable and versatile, axygen
deep well plate with
square wells
axymats minimize evaporation in a
wide range of temperatures in Pcr and
I Sizes - 1.5 ml and 2.0 ml
storage applications. These sealing
working volumes
mats are designed specifically for 96-
I SBS format allows
Well deep well plates with square wells.
sample purification
using robotic systems
I Ideal for Magentic bead
based purifications
because of its unique
base design
AXYPREP DEEP WELL PLATES
2.0 ml, square Well Plate
1.5 ml, square Well Plate
sealing Mat for square 96 Well Plates
PACK SIZES
50 plates/case
20 plates/case
10 mats/unit,
5 units/case
CATALOG NO.
P-2Ml-sQ-c
P-1Ml-sQ-c
aM-2Ml-sQ
33
axyPrep™ Mini Spin Basket
I Versatile design for
an exceptionally useful device for
many centrifuge-mediated sample
many applications
prep applications. The internal volume
- Mechanical filtration
is approximately 50 ml and is
- Gel filtration
excellent for applications, such as gel
filtration chromatography and nucleic
- Silica membrane
acid purification. This spin basket can
- Affinity membrane
be modified by adding porous frits or
I Can be capped during
retainer rings and membranes.
centrifugation†
I Molded-in support grid
Designed to be used with the axyPrep
1.5 ml or 2 ml capped microfuge tubes
I Fits all common
(#aP-mcT-1.5ml-c; #aP-mcT-2ml-c)†
microfuge tubes
when capping during centrifugation is
required. Will also fit most commonly
available 1.5 ml and 2 ml microfuge
tubes. Sold unfritted by the case (2,000
spin baskets). molded in medical grade
polypropylene. Polyethylene frits* and
retainer rings are sold separately.
†
caPPing The SPin BaSkeT requireS The uSe of eiTher of TheSe microcenTrifuge TuBeS.
* friT maTeriaL conSiSTS of 1.5mm Thick hyDroPhoBic PoLyeThyLene WiTh a 20µm Pore Size.
AXYPREP SPIN BASKETS
spin baskets (natural)
capped 1.5 ml microfuge tube (natural)
capped 2 ml microfuge tube (natural)
34
axygenBio.com
PACK SIZES
2,000 /case
4,000 /case
4,000 /case
CATALOG NO.
aP-MN-sBf
aP-Mct-1.5ml-c
aP-Mct-2ml-c
axyPrep™ G-Plates
I Unique, binary design
drip directors
I Secure fit in all assay
plates
I Secure fit within all PCR
plates
I 1 ml and 0.5 ml plates
I Sealable well design for
wet resin transport
I High clarity polystyrene
I SBS-format
The ultimate gel filtration media plate.
COLLECTION PLATES
This innovative plate has been designed specifically for centrifuge-
96-well collection Plate, 600µl
P-DW-500-c
96-well collection Plate, 1.1ml
P-DW-11-c
96-well collection Plate, 2.0ml
round Wells
P-DW-20-c
96-well collection Plate, 2.0ml
rectangular Wells
P-2mL-Sq-c
96-well collection Plate , 50µl
round Bottom
P-96-50r-c
96-well collection Plate, 50µl
‘v’ Bottom
P-96-50v-c
Plates = 5 plates/pack, 10 packs/case
mediated chromatography with gel filtration media, affinity resins and
wet resin slurries. The unique binary drip director allows this plate to be
securely nested within both standard assay plates and most Pcr plates
during centrifugation. This allows for stable and secure removal of
residual storage buffer during the initial spin (using an assay-type receiver
plate) followed by recovery of the purified samples directly into a Pcr
plate. These design features make the g-Plate ideal for applications such
as dye terminator removal. raised rings surrounding the top of each well
and broad, flattened discharge nozzles allow the wells to be sealed top
and bottom for transporting prehydrated resins. Plates are available
fritted* by the case (50 plates). Polyethylene frits are sold separately. Plates
are molded in high clarity polystyrene.
* friT maTeriaL conSiSTS of 1.5mm Thick hyDroPhoBic PoLyeThyLene WiTh a 20µm Pore Size.
AXYPREP G-PLATES PLATES
aXyPreP g-Plates 1.0ml (fritted)
aXyPreP g-Plates 0.5ml (fritted)
PACK SIZES
50 plates/case
50 plates/case
CATALOG NO.
gPf1.0-96-50
gPf0.5-96-50
35
argeNtiNa
australia
austria
BelgiuM
BoliVia
Brazil
Bulgaria
caNada
chile
chiNa
coloMBia
cyPrus
czech rePuBlic
deNMarK
doMiNicaN rePuBlic
egyPt
el salVador
eQuador
estoNia
fiNlaNd
fraNce
gerMaNy
greece
guateMala
hoNg KoNg
huNgary
icelaNd
iNdia
irelaNd
israel
italy
JaPaN
JordaN
KazaKhstaN
Korea
lithuaNia
Malaysia
Malta
MeXico
MoNteNegro
NeW zealaNd
NorWay
PaKistaN
Paraguay
Peru
PhiliPPiNes
PolaNd
russia
saudi araBia
siNgaPore
sloVaKia
south africa
sPaiN
sri laNKa
sWedeN
sWitzerlaNd
taiWaN
thailaNd
the NetherlaNds
triNidad
turKey
uKraiNe
uNited KiNgdoM
uNited states
uruguay
uzBeKistaN
VeNezuela
AXYGEN WORLD-WIDE
axygen inc is a global supplier, with
our products available in over 60
countries around the world.
axygen products are available
exclusively through a series
of distributors that hold inventory
locally. each is carefully selected
to offer technical information on
every axygen product and to
provide you with the highest
level of service.
WWW.AXYGENBIO.COM
sales: [email protected]
technical support: [email protected]
Warranty
axyPrep Products are designed for r&D and general laboratory use only.
axygen Biosciences makes no claims regarding the performance of these
products for clinical or diagnostic applications. axygen Biosciences warrants
that this product will perform as indicated for the specified application for a
period of up to 12 months from the date of receipt when stored in the
specified manner and used according to the instructions provided. in using
this product, the customer agrees that axygen Biosciences shall not be
held liable for any direct or indirect damages, including, but not limited to,
personal injury, property damage or lost profits (or other economic loss)
resulting from the use or inability to use this product. in the event that this
product fails to perform in the specified manner, remedial measures on the
part of axygen Biosciences shall be limited to the replacement of this
product and will be implemented at the discretion of axygen Biosciences.
AXYGEN BIOSCIENCES
33210 ceNtral aVeNue, uNioN city, ca 94587 usa
tel: 888-529-9246 (888-5-aXyBio) faX: 510-494-0700
WWW.aXygeNBio.coM