Lentiviral Technology
pGreenFireTM Sterol Response
Reporters
• Dissect Liver X Receptor (LXR)
Pathways
Cholesterol Homeostasis Pathway Reporters
VLDL
Macrophages
Hepatocytes
• Lentivector transcription
reporters available as Plasmids or
prepackaged Virus
CETP
Efflux
LXRs
CETP
Cholesterol
ABCA1
HDL
ABCG1
ApoA1
ApoE
Cholesterol
LXRs
• HepG2 stable cell lines for each
reporter also available
CYP7A1
SREBP1c
PPARs
• Dual reporter vector system to
quantitate Firefly Luciferase and
GFP for live cell imaging
Bile Acids
Fatty Acids
LDL
Liver X Receptors (LXRs) play a key role in lipid homeostasis. LXRs form
heterodimers with RXR to bind to LXR response elements (LXREs) in the target
promoter regions of cholesterol regulating genes. The LXRE is composed of two
direct repeat hexanucleotide sequences, separated by four bases. LXREs are found in
the promoters of key genes involved in cholesterol synthesis, transport, and
metabolism, as well as transport and synthesis of fatty acids. LXRs are implicated as
important sensors of sterol metabolism including the maintenance of normal
cholesterol balance by promoting sterol efflux from peripheral cells. Increases in
circulating HDL-cholesterol ultimately leads to hepatic sterol catabolism and
excretion.
The LXR Response Cholesterol Homeostasis Pathway Reporters (shown in green
boxes above) are designed to facilitate research into studying cholesterol efflux
mechanisms and for developing target gene-specific LXR agonists that could
regulate reverse cholesterol transport without increasing lipogenesis. Each
construct is available as a lentivector plasmid, as prepackaged virus and as a stable
reporter HepG2 cell line.
All of the pGreenFire Cholesterol Pathway Reporters contain a downstream
constitutive EF1-Puro cassette for stable cell line selection. The HepG2 reporter cell
lines are puromycin resistant stable reporter cell lines for the particular reporter
construct indicated. Each of the Liver X Receptor Response Elements (LXREs)
originate from the promoters of the gene indicated.
RSV 5’LTR
Ψ
AmpR
pGreenFire
Cholesterol
Reporter
pUC ori
mCMV
T2A peptide
3’ΔLTR
WPRE
Puro
www.systembio.com/greenfire
cPPT
copGFP
SV40 ORI
SV40 Poly-A
ABCA1
ABCG1
ApoE
Transcription
Response
Elements
Luciferase
EF1a
CETP
CYP7A1
LXRα
SREBP1c
ApoA1
PPRE
pGreenFire™ Sterol Response Reporters
Monitor Transciption Network Activity using both GFP and Luciferase
Cat#
CYTO113-PA-1
Cat# CYTO105-PA-1
Sterol
Response Elements
from Selected Promoters
ABCA1 LXRE GreenFire Reporter (HepG2 cell line)
Peroxisomes
Untreated Control
SREBP-1c (#TR100)
ApoE (#TR108)
600
Phase
RLU (x1000)
RLU (x1000)
500
300
100
GFP
100
0
0
0 nM
+ LXR Agonist TO-901317 (10μM)
0 nM
50 nM
400
100
40
0
0
0 μM
1 μM
10 μM
100 μM
0 nM
+ Fmoc-Leu
CETP (#TR109)
+ PPAR Agonist + Fmoc-Leu (100 μM)
Cyp7A1 (#TR105)
RLU (x1000)
350
34
200
10
0
GFP
26
18
100
Phase
1 μM
5 nM
+ TO-901317
GFP
RLU (x1000)
Phase
50 nM
200
800
Untreated Control
5 nM
+ TO-901317
LXRα (#TR102)
1600
RLU (x1000)
PPRE GreenFire Reporter (HepG2 cell line)
5 nM
+ TO-901317
PPRE (#TR101)
GFP
RLU (x1000)
Phase
300
0 nM
5 nM
50 nM
1 μM
0 nM
+ TO-901317
Cytosol (Red)
5 nM
+ TO-901317
Cat# CYTO100-PA-1
We Also Offer Custom Services
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System Biosciences, Inc.
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Mountain View, CA 94043
Toll Free: 888.266.5066
Fax: 650-968-2277
Email: [email protected]
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