Water In Aquaculture Systems To a Great Extent Water Quality Determines the Success or Failure of a Fish Farming Operation Water In Aquaculture Systems Fish Perform All Their Bodily Functions in Water Eat Breathe Grow Take In & Lose Salts Reproduce Pond Organisms Can Alter Water Chemistry 1. Nitrogen Cycle – Controlled by Bacterioplankton 2. Oxygen Cycle - Photosynthesis Replenishes Respiration Consumes 3. Water Chemistry Subject to: Both Diurnal and Seasonal Changes As Are Organisms Review – Components of Water Quality 1. Temperature 2. pH 3. Hardness 4. Alkalinity 5. Nitrogenous Compounds 6. Oxygen 7. Light 8. Particulates & BOD 9. Salinity 10. Feed 11. Fertilizer Water Quality Management 1. Large-Scale Solids Separation 2. Preliminary Considerations 3. Typical Pond Water Quality Data 4. Water Quality Sampling 5. Oxygen Management 6. Fertilization Policy 7. Water Exchange Policy 8. Calculating Water Exchange Large-Scale Solids Separation Sedimentation: Process Where Suspended Materials are Separated by Gravity – Used by Aquaculture Farms via Settling Ponds Goal: Remove 90% of Suspended Solids Design Parameters: Pond Cross-Sectional Area Detention Time, Depth, Overflow Rate Efficiency: Determined by Characteristics of the Water, Flow Variations – etc Avoiding Build-Up In Ponds Solids – Originating Due to Events in the Pond Sources: Unused Feed, Feces, Decaying Organisms Method 1: Method 2: Method 3: Method 4: Adequate Inflow, Effluent Thru Gates Adequate Longitudinal Slope Channeling Circulation – Reduce Dead Areas Preliminary Considerations Attempts to Improve Water Quality will Not Bear Fruit Immediately Pond Dynamics Change Only as Fast as You Can Input Changes You Must Remain Current Regarding Water Quality Must Maintain Data Base on Pond Parameters If Operating in Marine Environment, You Must Maintain Current Tide Tables Typical Pond Water Quality Data for Temperature Time Sampled: 05:30-06:00, 15:30-16:30 Frequency: At Temp Max/Min (2x Daily) Locations: Ponds, Canal Sections, Water Source Equipment: Thermometer, DO Meter, Multiprobes Depth: Bottom, Midway, Surface at Deep End Note: Pond Temps Vary Tremendously Typical Pond Water Quality Data for Salinity Time: Anytime for Ponds, But be Consistent Frequency: Daily, Estuary at High/Low Tide Location: All Ponds, Canal Sections, Estuary Equipment: Refractometer, Conductivity Meter Depth: Surface (Ponds), Various (Estuary, Canal) Note: High Tide – Higher Salinity, Low Tide – Lower Salinity Typical Pond Water Quality Data for pH Time: O2 Max/Min, 05:30-06:00 – 15:30/16:30 Frequency: 2x Daily – or If Color Changes in Pond Location: All Ponds Equipment: Any pH Meter w/ Compensating Probe Depth: Surface, Middle, Bottom (Ponds) Note: Should Increase in Afternoon, Decrease in Morning, Used to Calculate Ammonia Toxicity Typical Pond Water Quality Data for Secchi Disc - Color Time: Between 11:00 & 13:00 – be Consistent Frequency: Daily Location: All Ponds, Canals, Estuary Equipment: Secchi disk, Turbidimeter, Color Wheel Spectrophotometer Depth: Secchi (NA) Turbidometer / Spectrophotometer (Middle) – Color Wheel (Near Harvest Gate) Notes: Secchi at 2 depths (disappearing – reappearing) Secchi Correlated with Phytoplankton Counts – Color Coding Standardized Typical Pond Water Quality Data for TAN – Total Ammonia Nitrogen Time: 06:00 (low), 15:30/16:30 (High) Frequency: Weekly Location: All Ponds Equipment: Hach Drel 2-3000, Any Spectrophotometer Depth: Middle of Water Column Note: Use Same Samples as pH, Undertaken to Confirm Toxicity Potential of Fertilization with Urea or Di-Ammonium Phosphate – Unnecessary for Ponds Fertilized with Nitrates – Can be Used to Gauge Source Water, New or Old Pond Water for N2 Fertilization Typical Pond Water Quality Data for Orthophosphate Time: 06:00 (low), 15:30/16:30 (High) Frequency: Weekly Location: All Ponds Equipment: Hach Drel 2-3000 Depth: Middle of Water Column Note: Use Same Samples as pH, Undertaken to Confirm Toxicity Potential of Fertilization with Urea or Di-Ammonium Phosphate – Unnecessary for Ponds Fertilized with Nitrates General Comments: Water Quality Sampling Key to Getting Relevant Data is Proper Timing & Accuracy Sample each Water Source at the Same Time for Each Parameter – eg Temp Reading at Same Time Daily To Compare Data from One Pond with Another – Sampling Must Occur Within a Short Time Frame This Requires Established Infrastructure (Equipment Personnel, Transportation) to Allow This to Happen Areas of Concern: Oxygen Policy To Control Night-Time O2 Levels, Must Monitor O2 Trends over Several Days or a Week Problems: Overfeeding, Algal Die-Offs, Cloudy Days, No Wind Identify: Increases & Decreases Actions: Increase Water Exchange, Not Helpful if Source Water has Low O2 Levels Institute a Low O2 Policy Written and Followed by Managers Diel Oxygen Fluctuations Typical Pattern with O2 Max During Late Afternoon Problem: Differs from Pond to Pond & by Season Vertical Mixing & Aeration Can Also Impact During Dry Season – Faster Heating at Surface – Less Variation Example of a Low O2 Policy 3.0-2.5 ppm O2: No Late Night Feeding, Open Inflow Gates 25% 2.5-2.0 ppm O2: No Night Feeding, Open Inflow Gates 50% 2.0-1.5 ppm O2: Only Day Feeding, Open Inflow Gates 75% 1.5-1.0 ppm O2: No Feeding, Open Inflow Gates 100%, Remove 1 Exit Gate Board < 1.0 ppm O2: No Feeding, Open Inflow Gates 150%, Remove 2 Exit Gate Boards Aeration Most Aquatic Systems Employ Some Means of Oxygenating Holding Tanks Source Can be Purified O2 or Ambient Air Depending Upon Needs Typically This is a Three-Step Process: Transfer of Gaseous O2 to Gas-Liquid Interface Transfer Across the Gas-Liquid Interface Transfer from Interface into the Liquid Aerator Types Three Basic Types: Gravity, Surface, Diffuser Aerators use Energy to Increase Liquid Surface Area for Oxygen Transfer or Ensures Water with low DO Contacts O2 Mixing Increases Surface Area and Concentration Gradient Transfer Aeration also Helps Keep Suspended Particles in Suspension Gravity Aerators Use Energy Released when Water Loses Altitude to Increase Air-Water Surface Area, Increasing DO Concentration Turbulent Motion of Streams – Waterfalls Achieve This Effect Used in Large Applications, Tanks at Different Levels, Weirs Situated on Ponds or Raceways Gravity/Step/ Cascade Aerators Surface Aerators Agitate Water Surface Resulting in Larger O2 Transfer Rates Example: Pump Spraying Water into Air, Nozzle Aerators Surface Aerator Transfer Rate Depends On 1. Depth of Submergence 2. Rotor Speed 3. Rotor Diameter 4. Power Input per Unit Area 5. Liquid Being Aerated 6. Liquid Tank Dimensions & Shape 7. Oxygen Concentration Gradient 8. Aerator Design Diffuser Aerators Inject Air or O2 into Water as Bubbles O2 Transfer by Diffusion Bubbles Rise in Water Column Smaller Diameter Bubbles Better Diffuser Determines Bubble Size Diffuser Aerators Oxygen Transfer Depends on Steepness of Concentration Gradient Between Bubble and Surrounding Water, Percentage of O2 Saturation of Water Around Bubble Retention Time of Bubbles Rising in Water Column – Bubble Size, Gas Flow Rate Large Scale Diffuser Aerator Oxygen & Model Ecosystems I Most Aquatic Organisms Require Oxygenated Environment Some Submerged Aquatic Plants can Store O2 in Tissues Bacteria Remove O2 from H2O Directly Through Cell Wall Many Eukaryotes Have Specialized Blood Pigments for Carrying Oxygen Molds, Protozoans & Fungi can be Adapted to Anaerobic Environments Hence, Need for Aeration or Oxygenation in Aquatic Systems Oxygen & Model Ecosystems II In Microcosms & Mesocosms – All Aspects of Environment Simulated & Adequate Light for Photosynthesis Provided to Generate Baseline O2 Levels Aquarium Managers often Attempt to Mimic Conditions in The Environment Difficulties: Scaling & Ratio of Water Surface to Water Volume Do Not Allow This In Aquaria, Display Optimized—Volume Small & Biomass Density High Feeding Typically in Excess of Wild Equivalents Oxygen & Model Ecosystems III Hard to Simulate Adequate O2 Environment by Aeration Can be Achieved Using Liquid or Bottled O2 This Approach Expensive & Potentially Dangerous Model Ecosystems Typically Designed to Optimize Photosynthesis Areas of Concern: Pond Fertilization Total Algal Count Should be ~ 300,000 cells/ml If Less: Fertilization Required – If More: Reduce Feed or Flush System to Lower Count Secchi Disc Should be < 60 cm – If Less: Reduce Feeding – Flush System – If More: Fertilization Required Typical Fertilization Rate: 13 lbs Nutrilake / acre Plus 4.5 lbs Na3PO4 (TSP) / acre Response Will Take 2-3 days Areas of Concern – Water Exchange There are Two Ways to Manage Water Exchange: 1) Monitor Water Quality & Make Changes as Parameters go Bad or 2) Follow Guideline Where Water Exchange Increases with Biomass versus Method 1: Lag Time – Large-Scale Cyclic Patterns (valleys & peaks) Method 2: Less Lag Time – Smaller Peaks & Valleys Time Water Exchange Most Follow Guidelines for Water Exchange Where No Exchange Occurs Until Biomass Density is > 270 lbs / acre Often a Small Volume Added Routinely to Compensate for Evaporation and/or High Salinity Rate of Water Exchange Needs to Increase with Biomass Density – This Requires Population Sampling Cross Section Typical Inflow Gate CANAL SIDE TOP OF DIKE ATTACHMENT SLOT POND SIDE ↓ ↙ CULVERT PIPE ← PRIMARY FILTER FLASHBOARDS FILTER SLOT BAG FILTER Water Exchange Rate Changes with Density Daily Water Exchange as % of Total Pond Volume Biomass Density (Kg/m2) Water Exchange How does One Achieve Exchange? Answer: Depends on How Inflow & Exit Gates are Managed Two Approaches: Lower Flashboards in Exit Gates Increasing Output – Decreasing Volume Vary Overflow Rate Increasing Input – Increasing Volume Water Exchange Evaluate Volume of Water Flowing Over the Exit Gate The Larger this Volume, the Greater the Exchange Rate Volume can Vary Substantially for Various Reasons One Needs to Take the Average This Average Volume is the Flow Rate Water Exchange Example A Pond 8.0 hectares in Surface Area That has a Water Depth of 1.25 m Has a Volume of 100,000,000 L At an Average Flow Rate of 60 L/sec Over 24 hours the Total Volume of Water Exchanged is 5,184,000 liters The Water Exchange Rate is 5.18% Problem: Flow meters are Expensive – Need One to Obtain Reproducible Results Water Exchange Example II Question: What do You Do Without a Flow Meter? Answer: Estimate Using Discharge Equation Q = KLH1.5 Q: Discharge Volume L/sec – K: 1,838 a Unit Constant L: Width of Outflow – H: Outflow Height (depth) Imaginary Pond Q = (1,838) (1) (0.1)1.5 = 58.12 L/sec Or: 5,021,568 L/day ~5% Water Exchange – Very Close to Measured Volume of 5,184,000 or 5.18% Calculated Volume 97% of Measured Value Aquaculture Water Summary Maintenance of Water Quality Depends On: Knowledge of the Water Chemistry Interpretation of the Water Chemistry Proper Facility Design Including: Adequate Water Supply Suitable Quality of Source Water Your Ability (Skill & Resources) to Modify Conditions
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