Mouse Quick Genotyping
DNA Preparation Kit
Catalog # GK0100
50 mL of each buffer
Description:
The Mouse Quick Genotyping DNA Preparation Kit is a simple two buffer-two step protocol to extract DNA from mouse toes, ear punches,
tail snips or tissue samples. Extracted DNA is ready for PCR genotyping in 30 minutes or less. Each kit includes enough buffer to process
as many as 650 mice.
Contents:
Storage:
50 mL Tissue Lysis Buffer
Room Temperature, up to 1 year
50 mL DNA Stabilization Buffer
Protocol:
1. Collect mouse tissue (e.g. young mouse toes, ear punches, 2-3 mm tail snips or 25 mg tissue samples) into 0.2 mL microcentrifuge
tubes, 96-well plate wells or 1.5 mL minicentrifuge tubes.
2. Add 75 μL Tissue Lysis Buffer to each sample. Heat samples in Tissue Lysis Buffer to 95°C for 10-30 minutes. Cool samples to 4°C
by placing in the refrigerator for a few minutes.
3. Add 75 μL DNA Stabilization Buffer (or add equal volume of DNA Stabilization Buffer to Tissue Lysis Buffer if more than 75 μL was
used) to each sample and vortex. Add 0.5-2 μL of stabilized DNA sample to the PCR mastermix for a final reaction volume of 25 μL.
Notes:
✦
Mouse tail snips and tissue samples must be small. If larger tail snips or tissue samples are collected, the volume of buffer used needs
to be increased.
✦
Undissolved tissue does not interfere with PCR, however. Samples that are too large (i.e. DNA in excess) can inhibit PCR amplification.
✦
Using too much DNA in the PCR can cause band smearing. Consider optimizing the PCR by adding different volumes (concentrations)
of extracted DNA solution (e.g. 0.5 μL, 1 μL, 2 μL DNA solution).
✦
Extracted DNA samples should be stored at 4°C for up to 3 month or -20°C long term.
✦
DNA extracted using the Mouse Quick Genotyping DNA Preparation Kit cannot be used for Southern blot analysis.
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