CURRICULUM VITAE
Mami Oikawa, Ph.D.
Address: The Kaetsu Educational & Cultural Centre, Huntingdon Road, Cambridge CB3 0SH
Phone: +81 (0) 75-4675-7000
E-mail1: [email protected] E-mail2: [email protected]
Education
06/2011 – 03/2014
04/2011 – 03/2014
04/2009 − 03/2011
04/2005 − 03/2009
Ph.D. Student Trainee at RIKEN, Japan (Supervisor: Atsuo Ogura, D.V.M., Ph.D.)
Ph.D. Sc. (Science) in Biomedical Science at Tokyo Medical and Dental University
(Supervisor: Fumitoshi Ishino, Ph.D.)
M.Sc. in Biomedical Science at Tokyo Medical and Dental University
(Supervisor: Fumitoshi Ishino, Ph.D.)
B.Sc. in Animal Science, Nippon Veterinary and Life Science University
(Supervisor: Konosuke Okada, Ph.D.)
Professional training and employment
04/2016 – Present
01/2016 – 03/2016
12/2014 – 12/2015
04/2014 – 11/2014
Postdoctoral training at Dr. John Gurdon lab, Gurdon Institute, UK
Postdoctoral training at Dr. Kei Miyamoto lab, Kinki University, Japan
Postdoctoral training at Dr. John Gurdon lab, Gurdon Institute, UK
Postdoctoral training at Dr. Atsuo Ogura lab, RIKEN, Japan
Research experience
04/2016 – Present Postdoctoral Research (Supervisors: Drs. Jerome Jullien and John Gurdon)
·
Mechanism of sperm epigenetic information transmission throughout embryogenesis
01/2016 – 03/2016 Postdoctoral Research (Supervisor: Dr Kei Miyamoto)
·
Reprogramming of somatic nuclei towards totipotency is greatly facilitated by small
molecules through epigenetic alterations
12/2014 – 12/2015 Postdoctoral Research (Supervisors: Drs. Jerome Jullien and John Gurdon)
·
Mechanism of sperm epigenetic information transmission throughout embryogenesis
04/2014 – 11/2014 Postdoctoral Research (Supervisor: Dr. Atsuo Ogura)
·
Identification of the epigenetic factor of maternal Xist imprinting
·
Germ cell nuclear transfer for analysis of gene expression pattern in H3K9me2 regions
·
Generation of cloned mice from adult different neural cells by direct nuclear transfer
(Collaboration with Dr. Teruhiko Wakayama lab.)
·
Improvement of cloned blastocyst quality upon CHIR treatment of donor cells
(Collaboration with Dr. Pasqualino Loi lab, Italy)
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07/2011 – 03/2014 Ph.D. Research (Supervisors: Drs. Fumitoshi Ishino and Atsuo Ogura)
·
Large-scale nuclear transfer for analysis of establishment and timing of Xist imprinting
·
Effect of RNAi-mediated knockdown of Xist in female cloned embryos
·
Effect of embryonic manipulation on global gene expression profile in pre-implantation
embryos
·
Production of androgenetic haploid embryonic stem cells by two different methods
04/2009 – 06/2011 M.Sc. Research (Supervisor: Dr. Fumitoshi Ishino)
·
Identification of novel imprinting regions in tammar wallaby
·
Improvement of cDNA amplification methods
04/2007 – 03/2009 B.Sc. Research (Supervisor: Dr. Konosuke Okada)
·
Treatment of porcine oocytes with caffeine to facilitate their meiotic resumption and increase
the total cell number of embryos at the blastocyst stage after ICSI.
Membership of academic societies
The Society for Reproduction and Development (Japan)
The Molecular Biology Society of Japan
Honors and Awards
Apr, 2014
Mar, 2014
May, 2013
Mar, 2009
The JRD (J Repod Dev) Outstanding Paper Award in 2013
Meeting of Medical Research Institute, Presentation Award
Best Presentation Award, The 54th Annual Meeting of the Japan Society of Ova
Research
Best Student of Award 2009, Department of Animal Science, Nippon Veterinary
and Life Science University
Fellowships
10/2015 Postdoctoral Fellowship for Research Abroad, JSPS, Japan
07/2013 Fellowship for travelling, Association for Propagation of the Knowledge of Genetics, Japan
04/2013 Fellowship for Junior Research Associate, RIKEN, Japan
License
Artificial Insemination and Embryo Transfer in cow, in Japan
Publications
1.
Motomura K, Oikawa M, Hirose M, Honda A, Togayachi S, Miyoshi H, Ohinata Y, Sugimoto M,
Abe K, Inoue K, Ogura A. (2016). Cellular Dynamics of Mouse Trophoblast Stem Cells: Identification
of a Persistent Stem Cell Type. Biol Reprod. (in press)
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2.
Hatanaka Y, Inoue K, Oikawa M, Kamimura S, Ogonuki N, Kodama EN, Ohkawa Y, Tsukada Y,
Ogura A. (2015). Histone chaperone CAF-1 mediates repressive histone modifications to protect
preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A. 112:
14641-14646.
3.
Herberg S, Simeone A, Oikawa M, Jullien J, Bradshaw CR, Teperek M, Gurdon J, Miyamoto K.
(2015). Histone H3 lysine 9 trimethylation is required for suppressing the expression of an
embryonically activated retrotransposon in Xenopus laevis. Sci Rep. 5:14236.
4.
Inoue K, Oikawa M, Kamimura S, Ogonuki N, Nakamura T, Nakano T, Abe K, Ogura A. (2015).
Trichostatin A specifically improves the aberrant expression of transcription factor genes in embryos
produced by somatic cell nuclear transfer. Sci Rep. 5: 10127.
5.
Kurotaki YK, Hatanaka Y, Kamimura S, Oikawa M, Inoue H, Ogonuki N, Inoue K, Ogura A. (2015).
Impaired active DNA demethylation in zygotes generated by round spermatid injection. Hum Reprod.
30: 1178-1187.
6.
Mizutani E, Oikawa M, Kassai H, Inoue K, Shiura H, Hirasawa R, Kamimura S, Matoba S, Ogonuki
N, Nagatomo H, Abe K, Wakayama T, Aiba A, Ogura A. (2015). Generation of cloned mice from
adult neurons by direct nuclear transfer. Biol Reprod. 92: 81.
7.
Kamimura S, Hatanaka Y, Hirasawa R, Matsumoto K, Oikawa M, Lee J, Matoba S, Mizutani E,
Ogonuki N, Inoue K, Kohda T, Ishino F, Ogura A. (2014). Establishment of paternal genomic
imprinting in mouse prospermatogonia analyzed by nuclear transfer. Biol Reprod. 91:120.
8.
Oikawa M, Inoue K, Shiura H, Matoba S, Kamimura S, Hirose M, Mekada K, Yoshiki A, Tanaka S,
Abe K, Ishino F, Ogura A (2014). Understanding the X chromosome inactivation cycle in mice: a
comprehensive view provided by nuclear transfer. Epigenetics. 9: 204-211.
9.
Kamimura S, Inoue K, Ogonuki N, Hirose M, Oikawa M, Yo M, Ohara O, Miyoshi H, Ogura A
(2013). Mouse cloning using a drop of peripheral blood. Biol Reprod. 89: 24.
10. Oikawa M, Matoba S, Inoue K, Kamimura S, Hirose M, Ogonuki N, Shiura H, Sugimoto M, Abe K,
Ishino F, Ogura A. (2013). RNAi-mediated Knockdown of Xist Does Not Rescue the Impaired
Development of Female Cloned Mouse Embryos. J Reprod Dev. 59: 231-237. Selected to cover of
the issue and Outstanding Paper Award 2013
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Presentations
Oikawa M, Inoue K, Shiura H, Matoba S, Kamimura S, Hirose M, Tanaka S, Abe K, Ishino F, Ogura A.
Nuclear transfer provides a new picture of the X chromosome inactivation cycle. Oral session (selected),
Mouse Molecular Genetics 2013, Hinxton, U.K., September, 2013
Oikawa M, Inoue K, Matoba S, Ogura A. Male germ cell nuclear transfer for analysis of gene expression
pattern in LOCKs regions. Poster session, The 107th Annual Meeting of the Society for Reproduction and
Development, Obihiro, Japan, August, 2014
Oikawa M, Inoue K, Matoba S, Shiura H, Kamimura S, Hirose M, Melada K, Yoshiki A, Tanaka S, Abe K,
Ishino F, Ogura A. Nuclear transfer can provide a new picture of the X chromosome inactivatin in mice.
Poster session, The 1st Wakate BRC Conference, Tsukuba, Japan, June, 2014
Oikawa M, Inoue K, Matoba S, Shiura H, Kamimura S, Hirose M, Mekada K, Yoshiki A, Tanaka S, Abe K,
Ishino F, Ogura A. Understanding the X chromosome inactivation cycle in mice: a comprehensive view
provided by nuclear transfer. Poster session, RIKEN EPIGENETICS in Yokohama, Japan, February, 2014
Oikawa M, Inoue K, Matoba S, Shiura H, Ogonuki N, Abe K, Ishino F, Ogura A. Maternal Xist imprinting
is erased at implantation in mice. Poster session, The 106th Annual Meeting of the Society for Reproduction
and Development, Tokyo, Japan, September, 2013
Oikawa M, Inoue K, Matoba S, Shiura H, Kamimura S, Hirose M, Tanaka S, Abe K, Ishino F, Ogura A.
Large-scale nuclear transfer for analysis of X chromosome inactivation. Poster session, RIKEN “Noyori
Summer School”, Kobe, Japan, September, 2013
Oikawa M, Matoba S, Inoue K, Kamimura S, Ogonuki N, Shiura H, Sugimoto M, Abe K, Ishino F, Ogura
A. RNAi-mediated knockdown of Xist does not rescue the impaired development of female cloned mouse
embryos. Oral session, The 54h Annual Meeting of the Molecular Biology Society of Japan, Tokyo, Japan,
May, 2013
Oikawa M Nuclear transfer for analysis of the mechanisms of X chromosome inactivation in mammals.
Oral session, The 2nd Meeting of the Society for X chromosome, Sapporo, Japan, March, 2013
Oikawa M, Inoue K, Matoba S, Shiura H, Ogonuki N, Abe K, Ishino F, Ogura A. Germ cell nuclear transfer
for analysis of the timing of establishment of maternal Xist imprinting. Poster session, The 35th Annual
Meeting of the Molecular Biology Society of Japan, Fukuoka, Japan, December, 2012
Oikawa M, Inoue K, Matoba S, Shiura H, Ogonuki N, Abe K, Ishino F, Ogura A. Germ cell nuclear transfer
for analysis of establishment and maintenance of Xist imprinting. Oral session, The 105th Annual Meeting of
the Society for Reproduction and Development, Tsukuba, Japan, September 2012
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Oikawa M, Inoue K, Matoba S, Shiura H, Ogonuki N, Abe K, Ishino F, Ogura A. Germ cell nuclear transfer
for analysis of Xist expression pattern in pre-implantation embryos. Poster session, Young Researcher
Workshop 2012, Grant-in-Aid for Scientific Research on Priority Areas “THE GERMLINE”, Sendai, Japan,
July, 2012
Oikawa M, Kohda T, Ishino F. Improvement of cDNA amplification methods for microarray analysis.
Poster session, The 34th Annual Meeting of the Molecular Biology Society of Japan, Yokohama, Japan,
December, 2011
Oikawa M, Tomogane H, Ushijima H, Okada K. Treatment of porcine oocytes with caffeine facilitates the
timing of meiotic resumption and increases the total cell number of embryos at blastocyst stage after ICSI.
Poster session, The 102th Annual Meeting of the Society for Reproduction and Development, Nara, Japan,
September, 2009
Technical Experience
In vitro maturation of oocytes in mice and pigs
In vitro fertilization in mice and frogs
Culture of oocytes and embryos in mice and pigs
Collection of non-growing and growing oocyte in mice
Collection of sperm and spermatid in mice and frogs
Microinjection of mRNA and siRNA into oocytes in mice and pigs
Intracytoplasmic sperm injection (ICSI) using conventional or a piezo-procedure in pigs
ICSI and round spermatid injection in mice with a piezo-micromanipulator
Somatic cell nuclear transfer using a piezo-procedure or HVJ-mediated fusion in mice
Production of parthenogenetic embryos in mice
Production of androgenetic embryos in mice
Production of bimaternal embryos in mouse using HVJ-mediated fusion
Pronuclear transplantation in mice
Collection of pre-implantation embryos by flushing the oviducts in mice
Collection of blastocyst-stage embryos by flushing the uteri in mice
Production of chimeras in mice
Oviduct or uterine transfer of mouse embryos
Selecting females in estrus and checking plugs in mouse
Mouse embryo cryopreservation by vitrification
Mouse sperm cryopreservation
Establishment of mouse embryonic stem (ES) cells
Culture of ES cells, trophoblast stem cells and germline stem cells in mice
Culture of mouse primary tail fibroblasts
Preparation of mouse primary embryonic fibroblasts (e.g., for feeder cell layers)
Isolation of peri- and post-implantation embryos in mice
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Isolation of mouse primordial germ cells from the genital ridges
RNA-FISH for Xist
PCR
Quantitative RT-PCR
Amplification of RNA from single mouse pre-implantation embryos
Analysis of global gene expression profiles using microarray
Bisulfite sequencing
Restriction Fragment Length Polymorphism
Western blotting
Immunohistochemical staining of mouse oocytes and preimplantation embryos
MNase digestion of sperm chromatin
Separation of nucleosome using sucrose density gradient centrifugation
Chromatin immunoprecipitation of frog sperm
Separation of frog sperm using density gradient centrifugation
Making egg extract in frog
Validation of DNA replication of egg extract treated nuclei using Tetramethyl-Rhodamine-5-dUTP
Expression of ΔGeminin protein using E.coli
Extraction and purification of ΔGeminin protein
Outreach and volunteer activities
“My science life in Cambridge”, Invited speaker, The 121th Meeting of Japanese Society of Animal Science,
Tokyo, Japan, March, 2016
“The reason why I study in Cambridge” Oral presentation, “Hands-on Activity Program in UK” the
University of Tokyo, Cambridge, UK
“Where there’s a will, there’s a way.” Oral presentation, Connect the Dots Japan, Cambridge, UK
“Program to be programmed”, Oral presentation, UCL-Japan Young Challenge, Cambridge, UK
References
John Gurdon, Kt DPhil DSc FRS
Distinguished Group Leader, Wellcome Trust/CRUK Gurdon Institute Professor of University of Cambridge
Emeritus Professor of University of Cambridge
Address: Tennis Court Road, Cambridge, CB2 1QN, UK
E-mail: [email protected]
Atsuo Ogura, D.V.M., Ph.D.
Head of Bioresource Engineering Division, RIKEN Bioresource Center, Tsukuba, Japan
Professor of Tsukuba University
Address: 3-1-1, Koyadai, Tsukuba-shi, Ibaraki 305-0074, Japan
E-mail: [email protected]
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Fumitoshi Ishino, Ph.D.
Head of Department of Epigenetics, Division of Medical Genomics, Medical Research Institute, Tokyo
Medical and Dental University
Professor of Medical Research Institute
Address: 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan
E-mail: [email protected]
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