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#2867
Store at
-20ºC
Hexokinase II (C64G5) Rabbit mAb
Orders: 877-616-2355 (U.S.)
[email protected]
rev. 12/31/15
Entrez-Gene ID #3099
UniProt ID #P52789
For Research Use Only. Not For Use In Diagnostic Procedures.
Applications
W
Endogenous
Species Cross-Reactivity*
Molecular Wt.
Isotype
H, M, R, Mk
102 kDa
Rabbit IgG**
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
140
100
7
S-
RD
La
CO
Recommended Antibody Dilutions:
Western blotting
non-specific protein
Hexokinase II
80
1:1000
For product specific protocols and a complete listing
of recommended companion products please see the
product web page at www.cellsignal.com
60
50
Source/Purification: Monoclonal antibody is produced by
immunizing animals with a synthetic peptide corresponding to
the sequence of human
40
Background References:
(1) W
arburg, O. (1956) Science 123, 309-314.
50
(3) S mith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.
**Anti-rabbit secondary antibodies must be used to
detect this antibody.
200
Specificity/Sensitivity: Hexokinase II (C64G5) Rabbit mAb
detects endogenous levels of total hexokinase II protein. This
antibody also cross-reacts with a non-specific protein at 125
kDa in certain models.
(2) S emenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol.
35, 71-103.
He
kDa
A1
Background: Hexokinase catalyzes the conversion of glucose
to glucose-6-phosphate, the first step in glycolysis. Four
distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial
membrane and are critical for maintaining an elevated rate of
aerobic glycolysis in cancer cells (Warburg Effect) (1) in order
to compensate for the increased energy demands associated
with rapid cell growth and proliferation (2,3).
72
*Species cross-reactivity is determined by western blot.
30
β-Actin
40
Western blot analysis of various cell extracts using Hexokinase II
(C64G5) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970
(lower).
U. S. Patent No. 5,675,063
Tween is a registered trademark of ICI Americas, Inc.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween®20 at 4°C with gentle shaking, overnight.
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Applications: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide Species Cross-Reactivity: H—human M—mouse R—rat Hm—hamster
Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—Horse All—all species expected Species enclosed in parentheses are predicted to react based on 100% homology.