BioVision
08/16
For research use only
Anti- Histone H4
Rabbit Monoclonal Antibody
CATALOG NO:
A1139-25
ALTERNATIVE NAME:
H4FB, HIST1H4B, HIST1H4F, H4FN, H4FH
AMOUNT:
25 µg
CONCENTRATION:
1 mg/ml
CLONE:
RM212
IMMUNOGEN:
A peptide corresponding to the C-terminus of human Histone H4
HOST/ISOTYPE:
Rabbit IgG
SPECIES REACTIVITY:
Human
PURIFICATION:
Protein A affinity purified from an animal origin–free culture
supernatant
FORM:
Liquid
FORMULATION:
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
SPECIFICITY:
This antibody reacts to the Histone H4 protein, independent of
post-translational modifications. No cross reactivity with other
histone proteins.
STORAGE CONDITIONS:
For long term storage store at -20°C in small aliquots to prevent
freeze-thaw cycles
DESCRIPTION:
The nucleosome is made up of four core histone proteins (H2A,
H2B, H3 and H4) and is the primary building block of chromatin.
The N-terminal tail of core histones undergoes different
posttranscriptional
modification
including
acetylation,
phosphorylation and methylation. These modifications occur in
response to cell signal stimuli and have a direct effect on gene
expression.
APPLICATION:
Western Blot: 0.1 ug/ml – 0.5 ug/ml
ICC: 1 μg/ml - 2 μg/ml
ELISA: 0.2 ug/ml - 1 ug/ml
Multiplex: 0.1 ug/ml – 0.5 ug/ml
Note: This information is only intended as a guide. The
optimal dilutions must be determined by the user.
Western Blot of recombinant
Histone H2A, H2B, H3.1, H3.3 and
H4 proteins, using anti-Histone H4
antibody at 0.2 ug/ml RM212 is
specific to Histone H4.
Western Blot of A375, HEK293, HeLa
and SK-MEL-2 whole cell lysates,
using anti-Histone H4 antibody at 0.2
ug/ml, showed endogenous Histone
H4
Immunohistochemistry staining of
naïve
HepG2
cells
(Negative
control)
using
anti-His-Tag
antibody.
RELATED PRODUCTS:

Histone H4 (1-103 aa), Human recombinant (Cat. No. 7671-100)

Histone H4 Antibody (Cat. No. 3624-100)

Histone H4 Peptide, tetra-acetylated, biotin conjugate (Cat. No. 7144-01)

Histone H4 Blocking Peptide (Cat. No. 3624BP-50)
FOR RESEARCH USE ONLY! Not to be used on humans.
BioVision Incorporated
155 S. Milpitas Boulevard, Milpitas, CA 95035 USA
Tel: 408-493-1800 | Fax: 408-493-1801
www.biovision.com | [email protected]