Ming Tang
University of Illinois at Urbana-Champaign
Department of Chemistry
A127B CLSL, Box 41-6, MC-712
600 S Mathews Ave
Urbana IL 61801
Office: (217) 244-1583
Email: mingtang_at_illinois.edu
EDUCATION
Postdoctoral Research Associate
University of Illinois at Urbana-Champaign, Urbana, IL
Mentor: Prof. Chad Rienstra
2008 – present
Doctor of Philosophy, Chemistry
Iowa State University, Ames, IA
Advisor: Prof. Mei Hong
2003 – 2008
Bachelor of Science, Chemistry
Peking University, Beijing, China
Advisor: Prof. Chunhua Yan
1999 – 2003
RESEARCH INTERESTS
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Structural biology and biophysics of membrane proteins, protein complexes and aggregates.
Applications of NMR spectroscopy and X-ray crystallography on biological systems.
Development of novel solid-state NMR methods.
RESEARCH EXPERIENCE
Postdoctoral Research, University of Illinois at Urbana-Champaign
2008 – present
• Investigating the mechanism of the disulfide bond generation in E. coli by modeling membrane protein DsbB
in the lipid bilayers.
• Studying the conformation and dynamics of phosphoinositide in the membrane.
• Improved the sensitivity of large membrane proteins using paramagnetic relaxation enhancement.
• Characterized the cofactor ubiquinone in the active site of the disulfide bond generating enzyme system:
DsbB-DsbA (41 kDa).
• Determined high-resolution structures of membrane protein complex (DsbB-DsbA) combining solid-state
NMR and X-ray data.
• Collected high-resolution MAS NMR spectra of membrane proteins: bacteriorhodopsin (26 kDa), tissue
factor (28 kDa), cytochrome P450 98A3 (58 kDa), and cytochrome bo3 (144 kDa).
PhD Research, Department of Chemistry, Iowa State University
2004 – 2008
• Studied the oligomeric structure of the aggregates of a β-hairpin antimicrobial peptide PG-1 by 2D 13C-13C
correlation NMR.
• Elucidated that the driving force of arginine insertion into lipid membranes and pore formation by a cationic
antimicrobial peptide PG-1 using 13C-31P distance measurements.
• Determined the orientation of an antibacterial and antiviral peptide Retrocyclin-2 in the membrane using the
separated-local-field experiments and advanced simulations.
• Proposed and conducted experiments on the mutants of an antimicrobial peptide PG-1 in the membrane to
understand the peptide-lipid interactions.
Ming Tang
Curriculum Vitae
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Investigated the dynamics of the antimicrobial peptide PG-1 in the bacterial-mimetic membrane and its
relation to the peptide’s oligomeric structure and pore formation.
Demonstrated the use of trehalose to preserve the structure of lipid bilayers under lyophilization for
determining membrane protein insertion and topology.
TEACHING AND MENTORING EXPERIENCE
Postdoctoral Associate, University of Illinois at Urbana-Champaign
2008 – present
• Guided four graduate students with solid-state NMR instrument setup and data analysis.
• Judge for Undergraduate Research Symposium at University of Illinois at Urbana-Champaign on April 25th,
2009 and April 16th, 2011.
Teaching Assistant, Department of Chemistry, Iowa State University
2003 – 2007
• Lab instructor for CHEM 331L Organic Chemistry, and grader for CHEM 331 Organic Chemistry and
CHEM 301 Inorganic Chemistry.
• Graded assignments and exams for CHEM 561 Quantum Mechanics.
OTHER EXPERIENCE AND SERVICE
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Participated in writing the grant proposals for NIH R01 and P01.
Reviewer for Physical Chemistry Chemical Physics (PCCP), ChemComm and Organic & Biomolecular
Chemistry.
PUBLICATIONS / SCHOLARSHIPS
20. M. Tang, G. Comellas, C. M. Rienstra, “Advanced Solid-State NMR Approaches for Structure Determination
of
Membrane
Proteins
and
Amyloid
Fibrils”,
Acc.
Chem.
Res.
in
press
(2013).
http://dx.doi.org/10.1021/ar4000168
19. L. J. Sperling, M. Tang, D. A. Berthold, A. E. Nesbitt, R. B. Gennis, C. M. Rienstra, “Solid-State NMR Study
of a 41 kDa Membrane Protein Complex DsbA/DsbB”, J. Phys. Chem. B in press (2013).
http://dx.doi.org/10.1021/jp400795d
18. M. Tang, A. E. Nesbitt, L. J. Sperling, D. A. Berthold, C. D. Schwieters, R. B. Gennis, C. M. Rienstra,
“Structure of the Disulfide Bond Generating Membrane Protein DsbB in the Lipid Bilayer”, J. Mol. Biol. 425,
1670-1682 (2013). http://dx.doi.org/10.1016/j.jmb.2013.02.009
17. D.H. Zhou, A.J. Nieuwkoop, D.A. Berthold, G. Comellas, L.J. Sperling, M. Tang, G.J. Shah, E. J. Brea, L. R.
Lemkau, and C. M. Rienstra “Solid-state NMR analysis of membrane proteins and protein aggregates by proton
detected spectroscopy”, J. Biomol. NMR 54, 291-305 (2012). http://dx.doi.org/10.1007/s10858-012-9672-z
16. M. C. Brothers, A. E. Nesbitt, M. J. Hallock, S. G. Rupasinghe, M. Tang, J. Harris, J. Baudry, M. A. Schuler,
C. M. Rienstra, “VITAL NMR: Using Chemical Shift Derived Secondary Structure Information for a Limited Set
of Amino Acids to Assess Homology Model Accuracy”, J. Biomol. NMR 52, 41-56 (2012). http://dx.doi.org/
10.1007/s10858-011-9576-3
15. M. Tang, L. J. Sperling, D. A. Berthold, C. D. Schwieters, A. E. Nesbitt, A. J. Nieuwkoop, R. B. Gennis, C.
M. Rienstra, “High-resolution membrane protein structure by joint calculations with solid-state NMR and X-ray
experimental data”, J. Biomol. NMR 51, 227-233 (2011). http://dx.doi.org/10.1007/s10858-011-9565-6
14. M. T. Lin, L. J. Sperling, H. L. Frericks Schmidt, M. Tang, R. I. Samoilova, T. Kumasaka, T. Iwasaki, S. A.
Dikanov, C. M. Rienstra, R. B. Gennis, “A rapid and robust method for selective isotope labeling of proteins”,
Methods 55, 370-378 (2011). http://dx.doi.org/10.1016/j.ymeth.2011.08.019
13. M. Tang, D. A. Berthold, C. M. Rienstra, “Solid-State NMR of a Large Membrane Protein by Paramagnetic
Relaxation Enhancement”, J. Phys. Chem. Lett. 2, 1836–1841 (2011). http://dx.doi.org/10.1021/jz200768r
Ming Tang
Curriculum Vitae
2
12. M. Tang, L. J. Sperling, D. A. Berthold, A. E. Nesbitt, R. B. Gennis, C. M. Rienstra, “Solid-State NMR Study
of the Charge-Transfer Complex between Ubiquinone-8 and Disulfide Bond Generating Membrane Protein
DsbB”, J. Am. Chem. Soc. 133, 4359-4366 (2011). http://dx.doi.org/10.1021/ja107775w
11. M. Tang*, G. Comellas*, L.J. Mueller, and C.M. Rienstra, “High resolution 13C-detected solid-state NMR
spectroscopy of a deuterated protein”, J. Biomol. NMR 48, 103-111 (2010). *Equal contribution.
http://dx.doi.org/10.1007/s10858-010-9442-8
10. M. Tang,and M. Hong, “Structure and Mechanism of Beta-Hairpin Antimicrobial Peptides in Lipid Bilayers
from Solid-State NMR Spectroscopy”, Mol. BioSyst. 5, 317-322 (2009). http://dx.doi.org/10.1039/b820398a
9. M. Tang, A.J. Waring, and M. Hong, “Effects of Arginine Density on the Membrane-Bound Structure of a
Cationic Antimicrobial Peptide from Solid-State NMR”, Biochim. Biophys. Acta. 1788, 514-521 (2009).
http://dx.doi.org/10.1016/j.bbamem.2008.10.027
8. M. Tang, A.J. Waring, R.I. Lehrer and M. Hong, “Effects of Guanidinium-Phosphate Hydrogen Bonding on
the Membrane-Bound Structure and Activity of an Arginine-Rich Membrane Peptide from Solid-State NMR”,
Angew. Chem. Int. Ed. 47, 3202-3205 (2008). http://dx.doi.org/10.1002/anie.200705993
7. M. Tang, A.J. Waring, and M. Hong,“Arg Dynamics in a Membrane-Bound Cationic Beta-Hairpin Peptide
from Solid-State NMR”, Chembiochem 9, 1487-1492 (2008). http://dx.doi.org/10.1002/cbic.200800005
6. M. Tang, A.J. Waring, and M. Hong, “Phosphate-Mediated Arginine Insertion into Lipid Membranes and Pore
Formation by a Cationic Membrane Peptide from Solid-State NMR”, J. Am. Chem. Soc. 129, 11438-11446
(2007). http://dx.doi.org/10.1021/ja072511s
5. M. Tang, A.J. Waring, and M. Hong, “Trehalose-Protected Lipid Bilayers for Determining Membrane Protein
Insertion and Topology”, J. Magn. Reson. 184, 222-227 (2007). http://dx.doi.org/10.1016/j.jmr.2006.10.006
4. R. Mani, S.D. Cady, M. Tang, A.J. Waring, R.I. Lehrer, and M. Hong, "Membrane-Dependent Oligomeric
Structure and Pore Formation of a β-hairpin Antimicrobial Peptide in Lipid Bilayers from Solid-State NMR",
Proc. Natl. Acad. Sci. U.S.A., 103, 16242-16247 (2006). http://dx.doi.org/10.1073/pnas.0605079103
3. R. Mani, M. Tang, X. Wu, J. J. Buffy, A. J. Waring, M. A. Sherman, and M. Hong, "Membrane-Bound Dimer
Structure of a β-Hairpin Antimicrobial Peptide from Rotational-Echo Double-Resonance Solid-State NMR",
Biochemistry, 45, 8341-8349 (2006). http://dx.doi.org/10.1021/bi060305b
2. M. Tang, A.J. Waring, R.I. Lehrer, and M. Hong, “Orientation of a β-hairpin Antimicrobial Peptide in Lipid
Bilayers from 2D Dipolar Chemical-Shift Correlation NMR”, Biophys. J., 90, 3616-3624 (2006).
http://dx.doi.org/10.1529/biophysj.105.062075
1. M. Tang, A.J. Waring, and M. Hong, “Intermolecular Packing and Alignment of a β-Hairpin Peptide from 2D
Solid-State NMR”, J. Am. Chem. Soc., 127, 13919-13927 (2005). http://dx.doi.org/10.1021/ja0526665
INVITED TALKS / SEMINARS
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“Solid-State NMR Studies of the Interaction of a Cationic Membrane Peptide with Lipid Bilayers”.
University of Illinois at Urbana-Champaign, Department of Chemistry, Biochemistry Seminar, October 8,
2008
AFFILIATIONS
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American Chemical Society
The American Association for the Advancement of Science
Sigma Xi
2011 – present
2010 – present
2009 – 2010
CONFERENCE PRESENTATIONS
Ming Tang
Curriculum Vitae
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53rd Experimental NMR Conference, April 15-20, 2012, Miami, Florida. Talk: “High Resolution Structure
by Joint X-ray/NMR Calculations and Paramagnetic Relaxation Sensitivity Enhancement for a Large
Membrane Protein”.
46th Midwest and 39th Great Lakes Joint Regional ACS Meeting, October 19-22, 2011, St. Louis, Missouri.
Talk: “Membrane topology and mechanistic view of a disulfide bond generating membrane protein by
a structural model of membrane-embedded DsbB”.
242nd ACS National Meeting in Denver, August 28-September 1, 2011, Denver, Colorado. Sci-Mix poster:
“Structural Model of a Disulfide Bond Generating Membrane Protein DsbB in the Lipid Bilayers”.
35th Steenbock Symposium on Advances in Biomolecular NMR, June 26-28, 2011, Madison, Wisconsin.
Poster: “Structural Model of a Disulfide Bond Generating Membrane Protein DsbB in the Lipid Bilayers”.
Chicago Area NMR Discussion Group 2010 Meeting, November 6, 2010, Urbana, Illinois. Talk: “NMR
Studies of a Disulfide Bond Generating Membrane Protein DsbB in Lipid Environment”.
51th Experimental NMR Conference, April 18-23, 2010, Daytona Beach, Florida. Poster: “Solid-State NMR
Spectroscopic Characterization of the Endogenous Ubiquinone in the Disulfide Bond Generating Membrane
Protein DsbB”.
The Magnetic Resonance Gordon Research Conference, June 14-19, 2009, Biddeford, Maine. Talk: “SolidState NMR Studies of a Disulfide Bond Generating Membrane Protein Complex”.
The Magnetic Resonance Gordon-Kenan Research Seminar, June 13-14, 2009, Biddeford, Maine. Poster:
“Solid-State NMR Studies of a Disulfide Bond Generating Membrane Protein Complex”.
50th Experimental NMR Conference, March 29- April 3, 2009, Pacific Grove, California. Poster: “Structural
Determination of an Integral Membrane Protein DsbB by Solid-State NMR”.
NIH Roadmap Membrane Protein Production and Technologies Meeting, March 26-27, 2009, San Francisco,
California. Poster: “Structural Determination of an Integral Membrane Protein DsbB by Solid-State NMR”.
49th Experimental NMR Conference, March 9-14, 2008, Pacific Grove, California. Poster: “Interaction
between a β-sheet Antimicrobial Peptide Mutant and Lipids by Solid-State NMR”.
1st US-Canada Winter School on Biomolecular Solid State NMR, January 20-25, 2008, Stowe, Vermont.
Poster: “Interaction between a β-sheet Antimicrobial Peptide Mutant and Lipids by Solid-State NMR”.
49th Rocky Mountain Conference on Analytical Chemistry, July 22-26, 2007, Breckenridge, Colorado. Talk:
“Solid-State NMR Studies of the Insertion of a Cationic Membrane Peptide into Lipid Bilayers”.
47th Experimental NMR Conference, April 23-28, 2006, Pacific Grove, California. Poster: “MembraneInsertion and Conformation of Charged Residues in a β-Sheet Membrane Peptide by Solid-State NMR”.
46th Experimental NMR Conference, April 10-15, 2005, Providence, Rhode Island. Posters: “Solid-State
NMR Investigation of the Orientation and Membrane-Interaction of a Cyclic β-Hairpin Peptide” and
“Determination of Intermolecular Packing of a β-hairpin Peptide by 2D Solid-State NMR”.
15th International Society on Magnetic Resonance Conference, October 24-28, 2004, Ponte Verde Beach,
Florida. Poster: “Solid-State NMR Investigation of the Orientation and Membrane-Interaction of a Cyclic βHairpin Peptide”.
HONORS AND AWARDS
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ISOTEC travel sponsorship, 2008
Scholarship to the 1st US-Canada Winter School on Biomolecular Solid-State NMR, 2008
Frank J. Moore and Thoreen Beth Moore Fellowship, 2005
Cotton-Uphaus Award Finalist, 2005
46th ENC Student Travel Stipend, 2005
Ming Tang
Curriculum Vitae
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