Name _________________________________________________ PERIOD ______
Diffusion and Osmosis Practice
Below are semi-permeable bags filled with various concentrations of solutions placed in
beakers with various concentrations of solutions. Use this information to help answer the
questions below for each set-up.
• The solutions are prepared with distilled water.
• The semi-permeable membrane bag is permeable to glucose, a very small sugar
molecule.
• The semi-permeable membrane bag is NOT permeable to sucrose, a larger sugar
molecule.
For each set-up, you must:
1. Indicate whether the solution in the bag is isotonic, hypertonic, or hypotonic to
the solution in the beaker.
2. Draw a blue arrow for the net movement of water by osmosis (do not draw any
arrow if there would be no net movement of water).
3. Draw a green arrow for the net movement of solute by diffusion (do not draw
any arrow if there would be no net movement of solute).
4. Explain why you drew the arrows you did in terms of the properties of diffusion, osmosis,
and semi-permeable membranes.
50%
Sucrose
20%
Glucose
10%
Sucrose
20%
Glucose
The solution in the bag is
______________________________________
compared to the solution in the beaker
The solution in the bag is
______________________________________
compared to the solution in the beaker
Explain your arrows:
Explain your arrows:
Water goes __________ of the bag by osmosis.
Are there any arrows?
Would there be diffusion of sucrose?
WHY NOT?
Circle: YES or NO
Would there be diffusion of glucose?
Circle: YES or NO
40
grams
Sucrose
90
grams
Sucrose
100
grams
Sucrose
The solution in the bag is
______________________________________
compared to the solution in the beaker
90
grams
Sucrose
The solution in the bag is
______________________________________
compared to the solution in the beaker
Explain your arrows:
Water goes ____________ the bag by osmosis.
Are there any arrows? Why?
Would there be diffusion of sucrose?
Circle: YES or NO
Would there be diffusion of sucrose?
Circle: YES or NO
100 %
Glucose
90 %
Glucose
10 grams
Glucose
70 grams
Glucose
The solution in the bag is
______________________________________
compared to the solution in the beaker
The solution in the bag is
______________________________________
compared to the solution in the beaker
Explain your arrows:
Explain your arrows:
The blue arrow indicates water goes _______ the
bag by osmosis.
Will there be diffusion of glucose? YES or NO
The green arrow indicates glucose moving ______
the bag by diffusion.
The blue arrow indicates water moving ______
bag by osmosis.
Will there be diffusion of glucose? YES or NO
The green arrow indicates glucose moving _____
of the bag by diffusion.
60%
Sucrose
0g
glucose
60 %
Sucrose
100
grams
Glucose
The solution in the bag is
______________________________________
compared to the solution in the beaker
The solution in the bag is
______________________________________
compared to the solution in the beaker
Explain your arrows:
Explain your arrows:
Are there any arrows?
WHY NOT?
The blue arrow indicates water moving ______
bag by osmosis.
Will there be diffusion of glucose? YES or NO
Will there be diffusion of glucose? YES or NO
WHY NOT?
The green arrow indicates glucose moving _____
of the bag by diffusion.
80
grams
Sucrose
100%
Sucrose
0 grams
sucrose
200%
Sucrose
The solution in the bag is
______________________________________
compared to the solution in the beaker
The solution in the bag is
______________________________________
compared to the solution in the beaker
Explain your arrows:
Explain your arrows:
Water goes ____________ the bag by osmosis.
Water goes ____________ the bag by osmosis.
Would there be diffusion of sucrose?
Would there be diffusion of sucrose?
Circle: YES or NO
Circle: YES or NO
WHY?
WHY?
100%
Glucose
20 grams
Glucose
100 %
Glucose
10 grams
Glucose
The solution in the bag is
______________________________________
compared to the solution in the beaker
The solution in the bag is
______________________________________
compared to the solution in the beaker
Explain your arrows:
Explain your arrows:
Are there any arrows?
WHY?
THE FISH IS A FRESH
WATER FISH.
Would there be diffusion of glucose?
Circle: YES or NO
SALT WATER
FROM OCEAN
WHICH WAY WILL
WATER MOVE?
WHAT WILL HAPPEN
TO THE FISH?
0% sucrose
100% water
80 %
Sucrose
The solution in the bag is
______________________________________
compared to the solution in the beaker
Explain your arrows:
THIS FISH IS LIVES IN
THE OCEAN.
HE IS PLACED IN
FRESH WATER.
WHICH WAY WILL
WATER MOVE?
Water goes ____________ the bag by osmosis.
Would there be diffusion of sucrose?
Circle: YES or NO
WHAT WILL HAPPEN
TO THE FISH?
FRESH WATER
FROM OCEAN