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DNA Repair Enzymes
NEB carries an array of reliable DNA repair enzymes for use in multiple applications.
Properties of DNA Repair Enzymes
Enzyme
Substrate
Cleavage site
Termini created
from cleavage
5'
3'
terminus terminus
APE 1
AP sites
1st phosphodiester bond 5´ to the lesion
dR5P
OH
Endo III
Oxidized Pyrimidines, AP sites
1st phosphodiester bond 3´ to the lesion
P
PA
Endo IV
AP sites
1st phosphodiester bond 5´ to the lesion
dR5P
OH
Endo V
Deoxyinosine, mismatches, hairpin/unpaired
loop, flaps, pseudo Y structures
2nd or 3rd phosphodiester bond 3´ to the lesion
P
OH
Endo VIII
Oxidized Pyrimidines, AP sites
1st phosphodiester bond both 5´ and 3´ to the lesion
P
P
Fpg
Oxidized Purines, AP sites
1st phosphodiester bond both 5´ and 3´ to the lesion
P
P
hOGG1
Oxidized Purines, AP sites
1st phosphodiester bond 3´ to the lesion
P
PA
hNEIL1
Oxidized Pyrimidines and Purines, AP sites
1st phosphodiester bond both 5´ and 3´ to the lesion
P
P
T7 Endo I
Cruciform, Holliday junctions, mismatches,
herteroduplexes
1st, 2nd or 3rd phosphodiester bond 5´ to the mismatches
P
OH
T4 PDG
Pyrimidine Dimers
N-glycosidic bond of the 5´ Thymine of the dimer and the
1st phosphodiester bond 3´ to the AP site
P*
PA
UDG &
Afu UDG
Deoxyuridine
N-glycosidic bond
AP site (no break)
hSMUG1
Deoxyuridine, 5-hyroxyuracil, 5hydroxymethyluracil, 5-formyluracil
N-glycosidic bond
AP site (no break)
hAAG
Deoxyinosine, Alkylated purines
N-glycosidic bond
AP site (no break)
*A pyrimidine dimer still covalently attached.
Table Legend:
AP
apurinic/apyrimidinic sites
P
phosphate
OH
hydroxyl
dR5P
deoxyribose-5´-phosphate
PA
3´-phospho-α, β-unsaturated aldehyde
DNA Repair Glycosylases on Various Damaged Bases
Double-stranded DNA Oligos (34-mers)
ENZYME
AP:A
DHT:A 5-hmU:A 5-hmU:G I:T
6-MeA:T 8-0G:C
8-0G:G U:A
U:G
THYMINE GLYCOL:A
APE 1
++++ +
-
-
-
-
-
-
-
-
-
Endo III
++++ +
-
-
-
-
-
-
-
-
+
Tma Endo III
++++ ++
-
-
-
-
+
+
-
-
++
Endo IV
++++ +
-
-
-
-
-
-
-
-
-
Tth Endo IV
++++ +
-
-
-
-
-
+
-
-
-
Endo V*
+++
+
+
++++
+
++
+
+
++++
++
+
Repair
Enzymes
Double-stranded DNA Oligos (34-mers)
Endo VIII
++++ ++
-
-
-
-
-
-
-
-
+++
Fpg
+
+
-
-
-
-
++++
++++
-
-
+
hAAG
-
-
-
-
++++
-
-
-
-
-
-
hNEIL1
++++ ++
-
-
-
-
+
+
-
-
++
hOGG1
++
-
-
-
-
-
++++
+
-
-
-
T4 PDG
++++ -
-
-
-
-
-
-
-
-
-
UDG
N/A
-
-
-
-
-
-
-
++++
+
-
Afu UDG
N/A
-
-
-
-
-
-
-
++++
+
-
hSMUG1
N/A
-
+++
+++
-
-
-
-
++++
++++
-
Standard reaction conditions were used to titer the enzymes with the alternate base
*Nicks only, does not remove damage
Single-stranded DNA Oligos (34-mers)
Repair
Enzymes
ENZYME
AP
DHT
5-hmU
I
6-MeA
8-0G
U
THYMINE GLYCOL:A
APE 1
++
-
-
-
-
-
-
-
Endo III
++
-
-
-
-
-
-
-
Tma Endo III
++
+
-
-
-
-
-
-
Endo IV
-
-
-
-
-
-
-
-
Tth Endo IV
-
-
-
-
-
-
-
-
Endo V
+
-
-
++++
-
+
-
-
Endo VIII
+++
-
-
-
-
-
-
-
Fpg
+
+
-
-
-
+
-
+
hAAG
-
-
-
+
-
-
-
-
hNEIL1
+
+
-
-
-
-
-
+
hOGG1
++
-
-
-
-
+
-
-
T4 PDG
-
-
-
-
-
-
-
-
UDG
N/A
-
-
-
-
-
++++
-
Afu UDG
N/A
-
-
-
-
-
++++
-
hSMUG1
N/A
-
++
-
-
-
+++
-
Table Legend:
AP apurinic/apyrimidinic site. The AP site is created by treating a uracil containing oligo with UDG.
DHT 5,6-dihydrothymine
5-hmU 5-hydroxymethyluracil
I Inosine
6-MeA 6-methyladenine
8-OG 8-oxoguanine
U uridine
AP:A apurinic/apyrimidinic site base paired with adenine
DHT:A 5,6 dihydrothymine base paired with an adenine
5-hmU:A 5-hydroxymethyluracil base paired with an adenine
5-hmU:G 5-hydroxymethyluracil base paired with a guanine
I:T Inosine base paired with a thymine
6-MeA:T 6-methyladenine base paired with a thymine
8-OG:C 8-oxoguanine base paired with a cytosine
8-OG:G 8-oxoguanine base paired with a guanine
U:A uridine base paired with an adenine
U:G uridine base paired with a guanine
Level of Repair:
++++100%
+++ 50%
++ 10% –25%
+ <10%
– no detectable enzyme activity (<0.7%)
N/A not applicable