Protocol Sheet
InVitro Express
30 count sheet
OASIS® Grower Solutions
Tissue Culture Medium
in a Rigid Vessel
Achieve superior in vitro root
development, rapid handling at transplant
and faster greenhouse growth
35 count sheet
Product Dimensions
OASIS® InVitro Express (IVE) for rigid vessel comes in a sheet with 30 or 35 cells. The cells are separated by grooves running
the length and width of sheet. Each cell has a star shaped universal dibble hole that can accept very thin to thick caliper
micro cuttings.
• Sheet dimensions of 30 count and 35 count: 2.775" (7.05 cm) x 3.575" (9.08 cm) x 1" (2.54 cm).
• Each sheet of 30 count/35 count can hold up to 120 ml of media.
• Cell dimensions for 30 count sheet: 0.511" (1.30 cm) x 0.550" (1.40) x 1" (2.54 cm).
• Cell dimensions for 35 count sheet: 0.50" (1.27 cm) x 0.46" (1.17 cm) x 1" (2.54 cm)
• Rigid Vessel RV-750 dimensions: 3.54" (9cm) x 4.25" (10.8) x 3.40 (9.9 cm)
General Instructions for using OASIS® InVitro Express
in a rigid vessel for In Vitro Rooting (stage III)
Media Preparation
Place the IVE sheet in RV-750. Dispense the medium with or
without gelling agent in each vessel.
Media Volume
• If you are using this matrix for the first time on a new crop,
it is highly recommended to conduct a media volume
screening experiment to identify optimal media for your
specific crop. The lower the media volume used, the higher the air-filled pore space of the matrix.
• For initial screening, consider 100% (120 ml/sheet), 85% (100 ml/sheet), and
75% (90 ml/sheet) media holding capacity.
Note: Several crops are responding positively to liquid culture while some are not responding.
This is possibly due to the media composition or the liquid media itself. For this reason we are
recommending to run the first set of screening experiments without and also with gelling agent
to better understand the optimal culture media.
AUTOCLAVE
Autoclaving and Initiating Culture
• For details about experimental treatments for initial screening, refer to experimental
design sheet. After adding media with or without gelling agent, place the lids and
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InVitro Express
Autoclaving and Initiating Culture continued
autoclave using a standard cycle with slow, fluid exhaust. The autoclaving process allows the liquid media to move into
the cellular foam.
Note: If it is not possible to add culture medium before the autoclaving process, first autoclave the IVE sheets dry. Place the
autoclaved IVE sheet in the vessel and add hot (~100° C) sterile media without or with gelling agent over the top. Adding hot
media allows it to move into the cellular foam. Make sure the foam is saturated without any dry spots. Vessels can be stored
after cooling by enclosing the entire tray in a sterile, plastic bag.
• When ready to start the culture under hood, remove the lid, and insert the shoots into the dibble hole.
• Insert the cuttings approximately 1 - 1.25 cm deep so there is sufficient area in the
cube to promote uniform rooting. Note: inserting cuttings too deep can result in
compromised oxygen availability and slow down the root development.
1 - 1.25 cm
• Place the lid and seal it with a tape.
• Adjust the vent as required and follow the standard culture conditions.
For transplant
• When the plant material is ready for transplant, open the lid and tilt the
vessel sideways to remove the entire IVE matrix with the rooted plantlets
into a standard greenhouse tray with drain holes.
• Place the tray on a greenhouse bench and flush the matrix with greenhouse
fertilizer, using a hose and breaker. This process allows medium and
incorporating fertilizer in one step.
• Separate the cubes by breaking the foam along the grooves. Up pot the
plantlet with foam and roots intact.
• Acclimatize the plants as required. The acclimatization will be faster and
easier since the plantlets will have a stronger root system and minimal
damage during transplant.
FAQ
Q. Are the vessels and the filter membrane reusable?
A. Yes both vessels and filter membrane are reusable. If there is any mechanical damage,
then the filter membranes needs to be replaced.
Q. Can I place the shoots on the surface of the IVE growing medium without inserting them?
A. No, the shoots should be inserted into the matrix so the cut end of the shoot is exposed to the liquid media.
Q. How should I store the foam IVE growing medium?
A. The foam matrix can be stored at room temperature in a closed carton. During storage the foam should not
be exposed to light for a prolonged period of time because the UV light can make the foam brittle.
Q. How long can I store the foam matrix?
A. When stored properly as explained above, the foam matrix can be stored for more than a year.
OASIS® Grower Solutions, a division of Smithers Oasis
777 Stow Street Kent, OH 44240 USA
Phone: 855-585-4769 or 330-676-4486, Fax: 877-676-4409, www.oasisgrowersolutions.com
© Smithers-Oasis Company 2015. All rights reserved.
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