Magic™ Anti-PhosphoT (Phospho T) monoclonal antibody, clone SN203
Cat.No: DCABH-201822
Lot. No. (See product label)
PRODUCT INFORMATION
Product Overview
Rabbit monoclonal to Phosphothreonine
Specificity
This antibody reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated
threonine,
Target
PhosphoT
Immunogen
Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide
Isotype
IgG
Source/Host
Rabbit
Species Reactivity
N/A
Clone
SN203
Purification
Protein A affinity purified from an animal origin–free culture supernatant
Conjugate
Unconjugated
Applications
WB, IP, ICC, IHC, FC, ELISA
Procedure
Phospho-specific Antibodies
Format
Liquid
Size
100 μL
Buffer
PBS with 1% BSA and 0.09% sodium azide
Storage
Store at 2-8°C
Background
Introduction
Phosphorylation of threonine residues is associated with manygrowth factors and oncogene protein
kinases, and is important for cellsignaling in activation, proliferation and differentiation.
Proteinphosphorylation and dephosphorylation are basic mechanisms for themodification of protein
function in eukaryotic cells. Phosphorylation is arare post-translational event in normal tissue,
however, the abundance ofphosphorylated cellular proteins increases several fold following
variousactivation processes which are mediated through phosphotyrosine, phosphoserine or
phosphothreonine (p-tyr/p-ser/p-thr). Many signaltransduction pathways, such as the EGF, PDGF and
insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr
residuesupon binding of ligands to their receptors. T cell antigen receptor complexor the receptors for
some hemopoietic growth factors may stimulate thesephosphorylation associated kinases, and cells
transformed by viral oncogenescontain elevated levels of phosphorylated tyr/ser/thr. Immunoblotting
ofcellular proteins with antibodies directed against phosphoamino acids isadvantageous as it does not
involve 32P labeling, and can therefore beemployed to monitor alterations in phosphorylation of
specific proteins asthey occur in intact organs or the whole animal. Indeed, mono and
polyclonalantibodies directed against phosphorylated residues have been generated andfound useful
as analytical and preparative tools because they enable therapid identification, quantification and
immunoaffinity isolation ofphosphorylated cellular proteins.
Creative Diagnostics. All rights reserved
45-1 Ramsey Road Shirley, NY 11967, USA
Tel: 1-631-624-4882 Fax: 1-631-938-8221
E-mail: [email protected]
www.creative-diagnostics.com
1
Keywords
Phosphothreonine; threonine; Thr; T
Creative Diagnostics. All rights reserved
45-1 Ramsey Road Shirley, NY 11967, USA
Tel: 1-631-624-4882 Fax: 1-631-938-8221
E-mail: [email protected]
www.creative-diagnostics.com
2