757 © IWA Publishing 2012 Water Science & Technology | 65.4 | 2012 Potential impacts of on-site greywater reuse in landscape irrigation Y. Alfiya, O. Damti, A. Stoler-Katz, A. Zoubi, A. Shaviv and E. Friedler ABSTRACT This study investigated the effects of irrigation with different types of waters on soil, plants, and public health. The test plant was ryegrass grown in 12 planters filled with sandy loam soil and irrigated with three types of waters (4 planters for each type): freshwater, raw domestic light greywater (GW), and treated domestic light GW. The sodium adsorption ratio (SAR), EC, pH and alkalinity of the three types of irrigation waters did not differ significantly, suggesting that raw or treated light GW should not exhibit negative effects. Concentrations of anionic and cationic surfactants in the freshwater and the treated GW were about the same, while their concentrations in the raw GW were higher. Surfactant levels in the three drainage water types were low. Some accumulation of surfactants occurred in planters irrigated with raw and treated GW. The COD of the drainage water of planters irrigated with raw GW was higher than the COD of other two drainage water types. Although raw and treated GW contained faecal coliforms, they were hardly detected in the drainage waters. All plants did not show any signs of stress. This may be due to the fact that the GW originated mainly from showers and washbasins. Key words | greywater, landscape irrigation, microbial quality, on-site, ryegrass, SAR, soil Y. Alfiya A. Shaviv E. Friedler (corresponding author) Faculty of Civil and Environmental Eng., Technion – Israel Institute of Technology, Haifa 32000, Israel E-mail: [email protected] O. Damti GES ltd., Akko Industrial Park, Israel A. Stoler-Katz Ha’Emek St, Nofit 36001, Israel A. Zoubi Tahal Consulting Engineers ltd., 154 Begin Rd., Tel-Aviv 64921, Israel INTRODUCTION With growing demand for freshwater, greywater (GW) reuse for non-potable consumption becomes an attractive alternative water source. GW can be reused for toilet flushing (Nolde ; Friedler et al. ), laundry and car washing, fire protection, air conditioning (Lu & Leung ; Pidou et al. ) and for garden and landscape irrigation (Weil-Shafran et al. , ). GW reuse for landscape irrigation has the highest water saving potential in rural areas. In addition, garden farming with GW effluent may lead to poverty alleviation in poor peri-urban/rural neighbourhoods. GW constitutes 60–70% of the volume of domestic wastewater and only 2–3% and 22% of the ammonia and TSS, respectively (Almeida et al. ). Thus, it is considered to be less polluted than domestic wastewater. Nevertheless, GW contains elevated levels of COD, surfactants, salts and in some cases also boron (Friedler ; Weil-Shafran et al. ). GW further contains up to 105 cfu/100 mL and 107 cfu/mL faecal coliforms (FC) and heterotrophic plate count doi: 10.2166/wst.2012.903 (HPC), respectively (Casanova et al. ; Friedler et al. ; Gilboa & Friedler ). Pathogen bacteria may also be present in GW, such as Pseudomonas aeruginosa sp. (P.a. – mucous tissues pathogen), Staphylococcus aureus sp. (S.a. – skin pathogen) and Clostridium prefringes sp. (C.p. – faecal pathogen). Therefore, GW reuse may pose a public health hazard, and specifically, GW reuse for landscape irrigation may lead to accumulation and growth of pathogenic bacteria in the irrigated soils (Ottoson & Stenström ). Introducing high loads of organic matter, surfactants and salts by GW irrigation can negatively affect soil properties and harm the environment, especially when practiced above sensitive groundwater. This study endeavours to investigate and quantify the effects of irrigation with raw/treated domestic light GW (originating mainly from, showers and washbasins) on soil properties, on plants growth, and their potential public health and environmental implications in a comparative manner. 758 Y. Alfiya et al. | Potential impacts of on-site greywater reuse MATERIALS AND METHODS Experimental set-up Twelve rounded planters (soil depth 30 cm, diameter 24 cm (top), 22 cm (bottom)) were filled with 13 L of red loam sand (Hamra) with the following characteristics: porosity 40%, field capacity 20%, clay 3.75%, silt 2.50%, sand 93.75%; CaCO3 2.1%; density 1.3 g/cm3; organic matter content 0.4%, cation exchange capacity 3.0 meq/100 g; pH in water 6.7 (1:2 ratio 1 portion soil and 2 portions distilled water). Ryegrass (Lolium perenne) was selected as the model plant due to its high growth rate, high water and nutrients uptake and the possibility to perform multiple harvests (Hall ). Each planter was seeded with 15–20 seeds of ryegrass. Planters were placed outdoors on benches in a random order, in order to avoid location bias. They were irrigated with three types of water: freshwater (four planters), raw domestic light GW (four planters), and RBC (Rotating Biological Contactor) treated light GW effluent (four planters). The GW was collected from 14 flats in a house accommodating married students (some with young children), within the Technion Campus. The collected greywater was treated in a pilotscale treatment plant situated in the basement of the building and consisting of an equalisation basin, RBC (rotating biological contactor), followed by sedimentation basin. A detailed description of the raw GW, the treatment system and the treated GW can be found in Friedler et al. () and in Gilboa & Friedler (). The raw and treated GW were transported to experiment site on the date of irrigation. The time elapsed between collection of the two types of GW and commencing irrigation was very short (not more than 30 min). The plants were irrigated for 144 d from 27/4/06 to 18/ 9/06 (spring–summer). During this period, no precipitation Figure 1 | The schedule of irrigation, washing and harvesting as implemented. Water Science & Technology | 65.4 | 2012 occurred. They were irrigated by drip irrigation, each planter by two drippers of 2 L/h, which were placed very close to the soil surface. Irrigation was performed every 3 days on average, with increasing volumes following plant growth (Figure 1). Each planter was connected to a flexible drainage hose. About every fortnight (days 25, 39, 52, 70, 83 and 96, after planting) the planters were irrigated with excess water in order to wash the soils of accumulated substances (3.5– 6 l, Figure 1). On these occasions the whole volume of the drainage water from each planter was collected in a vessel and analyzed in the laboratory. Soluble fertilizer solution was added to the three types of irrigation water, with the following concentrations: 2 mg-P/L potassium phosphate dibasic, 30 mg-N/L ammonium sulfate, and 50 mg-K/L potassium sulfate. Two weeks before each harvest the dose of ammonium sulfate was doubled, in order to meet the higher demand of the plants. Plants were harvested four times during the experiment (days 43, 68, 89 and 111 after planting). During the harvest, the biomass of the plants was cut from 5 cm above the soil surface, collected from each planter separately, and weighed (wet weight) within 1–2 h. Analysis methods The three types of irrigation water and the drainage water from each planter were analyzed according to the Standard Methods (APHA et al. ) for the following parameters: total suspended solids (TSS), volatile suspended solids (VSS), chemical oxygen demand (COD), total ammonium nitrogen (TAN), anionic surfactants as methylene blue active substances (MBAS). Cationic surfactants (CS) were measured by extraction with bromophenol blue and chloroform (Kornecki et al. ). Alkalinity was measured with Gran titration (Stumm & Morgan ). Turbidity was 759 Y. Alfiya et al. | Potential impacts of on-site greywater reuse measured with Lamotte 2020e nephlometer (method 2310B). Nitrate (NO3), nitrite (NO2), phosphate (PO43) and sulfate (SO42) were measured using Ion Chromatograph DIONEX AS4. Total organic carbon (TOC) and total nitrogen (TN) were measured with Multi N/C 2000 analyzer (Analytik-Jena). Na, K, Ca and Mg were measured by ICP (Perkin-Elmer, Optima 3000 DV). Bacterial quality was characterized by Heterotrophic Plate Count (HPC) (method 9215C), Faecal Coliforms (FC) (method 9213D), Pseudomonas aeruginosa sp. (P.a.) (method 9215E) and Staphylococcus aureus sp. (S.a.) (method 9215E). The latter two bacteria species are pathogens that are commonly found in light GW (Gilboa & Friedler ). RESULTS AND DISCUSSION Irrigation water characteristics Raw GW quality exhibited high variability especially in key parameters, such as TSS, VSS, Turbidity, COD and TOC (Table 1). Treated GW quality was more stable, but still showed some variability. Nevertheless, both the raw and treated (un-disinfected) GW exhibited high variability, as expressed by the large standard deviations. This high variability is common and reported in the literature (Friedler et al. ; Gilboa & Friedler ). The variability exhibited in the freshwater quality, that as expected was much lower, resulted from the fact that the source of water of Haifa varies from surface water to groundwater and vice-versa. pH, EC, Na2þ Ca2þ and Mg2þ concentrations did not exhibit large differences between the three types of water. Consequently, the sodium adsorption ratio (SAR) did not differ significantly, rising from 2.88 in freshwater to 3.23 (12% rise) in raw GW and 3.12 in treated GW (8% rise). Thus, in regard to SAR, irrigation with either raw or treated light GW is not expected to harm the soil structure. Alkalinity of the freshwater was the highest while in the raw GW and treated GW it was somewhat lower. As the GW stream did not contain laundry and dishwasher streams, no alkalinity addition occurred during water use within the households. In addition, some of the household products which may enter this type of GW are acidifying (e.g. hydrochloric acid, boric acid). The difference between the alkalinity of the raw and treated GW was not found to be significantly different (p 0.7624). Minor differences between anionic and cationic surfactant concentrations in the freshwater and the treated GW Water Science & Technology | 65.4 | 2012 were noticed, but as expected, their concentrations in the raw GW were higher. The RBC efficiently removed both cationic and anionic surfactants. This is an important point, as accumulation of surfactants in soils may lead to hyrophobicity (Gross et al. ). The loads of anionic surfactants in the raw and treated GW were 113 and 0.43 μg/(kgsoil d), respectively. Sodium and sulfate concentrations in the raw and treated GW were higher than in freshwater (∼10% and 70% higher for sodium and sulfate, respectively). The RBC did not remove phosphorus and nitrogen compounds, but succeeded to transform some of the TAN to nitrate. The overall N:P:K contribution of the irrigation waters (expressed as mg-N/L:mg-P/L:mg-K/L), including contribution of fertilizers, of the freshwater, treated GW and raw GW were 48:7:88, 55:7:98 and 51:6:96 respectively. These differences between the irrigation waters were marginal (± 10%). Generally, the microbial quality of all three types of irrigation waters were within the range reported in the literature (Table 1). As expected, no FC were found in the freshwater. In the raw GW, FC concentrations were 3 orders higher than in the treated GW. HPC concentration in the raw GW was the highest (∼3 orders of magnitude higher than in freshwater), but, in the treated GW, HPC was only about 0.5 order of magnitude lower than in the raw GW. This is due to the fact that residual organic matter in the treated GW enabled bacterial growth. In 60% of the freshwater samples P.a. was found. Pathogen bacteria should not be present in tap water, especially when indicator bacteria like FC are not found. Thus, the presence of P.a. could be artificial (i.e. owing to contamination of the samples). In the raw GW, P.a. was in the order of 104 cfu/100 mL, while the treated GW contained two orders less. S.a. was found only in one third of the raw GW samples, while in the other two types of irrigation waters it was not found. Following these findings, it can be postulated that raw light GW and even the treated GW may pose a health risk, especially if irrigated by sprinklers, due to possible body contact. This stresses the need for proper disinfection prior to reuse. Finally, it should be noted that the microbial quality of the irrigation and drainage waters were sampled for two bacterial indicators and two bacterial pathogens, while other pathogens could potentially be present (e.g. viruses). Quality of the drainage water The EC of the three types of irrigation waters was around 1 mS/cm, while the EC of the drainage waters was, as | 760 Table 1 Irrigation water characteristics (n ¼ 6) Parameter Fresh-water Raw GW GW Parameter water Raw GW GW 0.987 0.232 1.044 0.220 1.081 0.142 TAN (mg-N/L) AVG range SD 0.07 0.08 3.46 (1–75) 3.27 6.95 12.9 pH AVG range SD 7.74 0.28 7.24 (6.4–10) 0.37 7.64 0.56 NO3 (mg-N/L) AVG range SD 1.97 1.54 1.21 (0.1–17) 1.48 4.53 2.54 Alkalinity (mgCaCO3/L) AVG range SD 160 80 112 39 122 21 NO2 (mg-N/L) AVG range SD 1.2 0.6 4.9 (0.04–0.4) 7.2 2.1 1.6 TSS (mg/L) AVG range SD 8 1 52 (2–1,070) 28 19 8 TN (mg/L) AVG range SD 2.2 1.1 10.5 (0.1–128) 7.5 11.2 14.3 VSS (mg/L) AVG range SD <8b 45 (6–413) 22 18 7 PO43 (mg-PO4/L) AVG range SD 0.6 0.9 1.9 (0.1–49) 1.0 2.1 1.0 Turbidity (NTU) AVG range SD 1.0 0.38 28 (20–279) 19 1.7 0.53 SO42 (mg-SO4/L) AVG range SD 89 51 157 (0.5–72) 146 149 147 COD (mg-O2/L) AVG range SD 40 15 174 (7–2,570) 30 74 27 Naþ (mg/L) AVG range SD 114 6.8 125 (7.4–480) 4.3 123 6.8 TOC (mg-C/L) AVG range SD 2.94 0.61 27 (73–93) 7.7 5.8 1.0 Kþ (mg/L) AVG range SD 13.8 12.7 18.7 (0.2–24) 17.1 21.1 19.0 Cationic surfactants (mg/L) AVG range SD 0.10 0.11 0.64 (NA) 0.30 0.14 0.11 Ca2þ (mg/L) AVG range SD 64 14.7 57 (3.5–58) 7.3 61 6.9 Anionic surfactant (mgMBAS/L) AVG range SD 0.07 0.07 2.87 (1.4–56) 2.20 0.08 0.07 Mg2þ (mg/L) AVG range SD 34 3.2 33 (1.1–34) 2.6 34 2.9 SAR AVG range 2.88 3.23 (3–7) 3.12 SD 0.43 0.25 0.28 P.a. (cfu/100 mL) AVG range 2.9 × 101 3.1 × 102 SD %0 3.4 × 101 40 3.0 × 104 (3 × 103–3 × 104) 3.9 × 104 33 AVG range 0 SD %0 0 100 FC (cfu/100 mL) HPC (cfu/mL) AVG range B.D.b SD %0c 0 100 AVG range 3.9 × 104 SD %0 2.9 × 104 17 3.0 × 105 (2 × 102 6 × 106) 3.8 × 105 0 8.8 × 106 (8 × 106 3 × 107) 7.9 × 106 0 1.7 × 102 2.3 × 102 0 1.7 × 106 2.5 × 106 0 1.2 × 104 (2 × 103 1 × 104) 1.8 × 104 67 0 0 100 Range reported in the literature (Compilation of: Fox et al. 2002; Ottoson & Stenström 2003; Friedler 2004; Jefferson et al. 2004; Gross et al. 2005; Palmquist & Hanaeus 2005; Friedler et al. 2006; Vinnerås et al. 2006; Briks & Hills 2007; b 2012 Below detection. %0 – Proportion of samples in which concentrations were below the detection limit. | c 65.4 Ghunmi et al. 2008; Gilboa & Friedler 2008; Jamrah et al. 2008; Winward et al. 2008; Meinzinger & Oldenburg 2009). | a S.a. (cfu/100 mL) 5.4 × 102 60 Water Science & Technology AVG range SD Potential impacts of on-site greywater reuse EC (mS/cm) | a RBC treated Fresh- Y. Alfiya et al. RBC treated 761 Y. Alfiya et al. | Potential impacts of on-site greywater reuse expected, significantly higher (Figure 2(a)). The excess irrigation water washed salts that accumulated in the soil during normal irrigation. The EC of the drainage water of planters irrigated with raw GW was higher than that of planters irrigated with treated GW and with freshwater. Furthermore, large variability was observed in the EC values of the drainage water between the four planters that were irrigated with raw GW. The pH of the drainage water at the beginning of the experiment was low: 6.19, 6.00 and 6.68 for planters irrigated with freshwater, treated GW and raw GW respectively (Figure 2(b)). The pH values rose during the experiment, reaching their final values after 60–70 days, at the end of the experiment the pH values were 7.20, 7.11 and 7.19 (freshwater, treated GW and raw GW, respectively). Travis et al. () observed the same pattern of rising pH after irrigation with freshwater treated GW and raw GW. Differences in pH values between the drainage waters of different irrigation treatments were marginal. At the beginning of the experiment the alkalinity of the drainage waters was lower than that of the irrigation waters (Figure 2(c)), with values of 74, 21 and 45 mg-CaCO3/L in the drainage water of the planters irrigated with freshwater, treated GW and raw GW respectively. It should be noted that the alkalinity of 21–45 mg-CaCO3/L is very low and under these conditions the soil may suffer from pH instability. During the experiment, the alkalinity gradually increased, generally reaching the values of the irrigation Figure 2 | Water Science & Technology | 65.4 | 2012 waters towards the end of the experiment. This may explain the changes in the pH. Differences between the alkalinity values of the drainage waters of the different irrigation treatments were not significant. Alkalinity expresses the buffer capacity of liquids. Therefore, irrigation had a positive effect on the buffer capacity of the soil. The COD of the drainage water of the planters irrigated with raw GW was higher than the COD of the other two drainage water types, with concentrations generally equaling the values in the irrigation waters (Figure 2(d)). The difference in the COD of the drainage water of the planters irrigated with freshwater and treated GW was marginal. TOC in the drainage waters exhibited the same general trend (data not shown), lying in the range of 15–30 mg-C/L. COD leaching from fields irrigated with GW can contaminate surface water and groundwater, and promote growth of pathogen bacteria. Anionic and cationic surfactant concentrations in the drainage water of all types of irrigation water were low and decreased during the experiments to close-to or below the detection limits of the analytical methods used (Figure 2(e)–(f)). This can result from gradual development of the microbial community in the soil that can degrade the surfactants added by the irrigation waters. Nevertheless, minor (statistically significant) accumulation of surfactants was observed in the soil, 0.35 and 0.73 μg/ (kgsoil d), anionic surfactants in planters irrigated with treated and raw GW, respectively, and 9.5 μg/(kgsoil d) cationic surfactants in planters irrigated with raw GW. Considering EC (a), pH (b), alkalinity (c), COD (d), anionic surfactants (e) and cationic surfactants (f) in irrigation and drainage waters. 762 Y. Alfiya et al. | Potential impacts of on-site greywater reuse the above accumulation, the loads of surfactants exerted by raw GW (see irrigation quality characteristics, above), and the amounts lost by drainage, the calculated degradation of surfactants was 99 and 46% respectively for anionic and cationic surfactants in the soil of the planters irrigated with raw GW. As expected, in most of the samples of drainage water of the planters irrigated with freshwater, FC were not detected (Figure 3(a)), while only on one occasion was low FC level detected in one planter (out of four replicates). Thus, it can be assumed that the sample was contaminated. The treated GW contained FC (1.7 · 102 ± 2.3 · 102 cfu/100 mL), while only in three samples (at days 39, 109 and 144) from the same planter drainage (out of four replicates), FC were detected at concentrations lower than 10 cfu/100 mL (Figure 3(b)). This was probably, due to the wall effect. Although the raw GW exerted a high load of FC on the planters (3 · 105 ± 3.8 · 105 cfu/100 mL), only on two occasions were FC detected in the planters’ drainage water: on day 52 in planter 12 drainage (1 · 101 cfu/100 mL) and on day 69 in planter 10 drainage (2 · 103 cfu/100 mL). The fact that hardly any FC leached out of the planters may be due to the fact that the environment in the root zone (represented by the soil in the planters) is hostile to FC and thus they do not proliferate. This finding is supported by Gross et al. () who found that FC did not survive in soil irrigated with raw greywater. Nevertheless, if the load of FC were high enough they could get to the leachate as well. HPC concentrations both in the irrigation and drainage waters were much higher (6–7 orders of magnitude) than Figure 3 | Water Science & Technology | 65.4 | 2012 those of FC for all irrigation water types (Figure 3(d)–(f)), and HPC were present in all samples. HPC concentrations in the drainage waters were in the range of 106 cfu/mL, regardless of the type of irrigation water. In planters irrigated with freshwater HPC, concentrations in the drainage water were higher than in the irrigation water, in planters irrigated with treated GW it was about the same, while in planters irrigated with raw GW, HPC concentration in the drainage water was somewhat lower than in the irrigation water. Thus, it can be postulated that HPC present in the drainage water are not necessarily the ones that were introduced to the soil by the irrigation water and may originate from a population that developed in the soil within the planters. S.a. was found only in two samples (33%) of raw GW. Consequently, its concentration in all drainage waters was always below its detection limit (<1/100 mL). Although P.a. is widely known as an opportunistic pathogen for humans and animals, it can be found ubiquitously in nature from sources as diverse as water, soil and plants (Alonso et al. ). Thus, although it was present in the irrigation waters, it was not possible to study its fate and transport in this experiment. Influence of the irrigation on plant growth All plants, including the ones irrigated with raw GW, did not show any signs of disease or phytotoxicity throughout the experimental period (144 d). This is contrary to the findings of Eriksson et al. () who reported that bathroom GW was toxic to algae, and kitchen and laundry Geometric means of FC (a–c), HPC (d–f) concentrations in the irrigation and drainage waters. NM – Not measured; %zero – proportion of samples where bacteria were below detection limit; vertical lines (HPC) indicate one SD. 763 Figure 4 Y. Alfiya et al. | | Potential impacts of on-site greywater reuse Water Science & Technology | 65.4 | 2012 Irrigation water effects on plant growth – average weight of harvested biomass (a); plant growth rate (b). GW were toxic to algae and willow cuttings. Weil-Shafran et al. () observed chlorosis of lettuce leaves following irrigation with laundry GW. The GW in this study originated mainly from showers and washbasins and hardly contained laundry GW. This could explain why no phytotoxicity was observed. Nevertheless, irrigation with treated GW resulted in biomass yield higher than the other two types of irrigation water (statistically significant two-tailed t-test; p 0.001 over freshwater, p 0.0023 over raw GW; (Figure 4(a)). The difference between yields of plants irrigated with freshwater and those irrigated with raw GW was very small and not proven to be statistically significant. No obvious reason for this difference was found. Pinto et al. () reported that no detrimental effects of irrigating Silver-beet plants (a common garden plant) with raw GW were observed, although there was a slight reduction in biomass. Growth rate of all plants slightly increased with time (Figure 4(b)), with growth rate of plants irrigated with treated GW being higher (statistically significant) than the ones of plants irrigated with freshwater and raw GW. The higher yield of plants irrigated with treated GW may be related to higher levels of nutrients, although nutrient levels in the three types of irrigation water were about the same, with slightly higher levels in the raw and treated GW (although differences between nutrient levels were not found to be statistically different, see irrigation water characteristics, above). The lower growth rate of the plants irrigated with raw GW may be attributed to compounds present in raw GW that can inhibit the plants' growth (and degraded during treatment). However, no evidence was found to support this hypothesis. CONCLUSIONS This study investigated the effects of irrigation with raw/treated domestic light GW (originating predominantly from, showers and washbasins) on soil properties, plant growth, and public health. The SAR, EC, pH and alkalinity of the three types of irrigation water did not exhibit large differences, suggesting that regarding these parameters irrigation with raw or treated light GW is not expected to harm soil structure or have detrimental effects on plants. Anionic and cationic surfactant concentrations in freshwater and treated GW were about the same, being higher in the raw GW (as expected). Surfactant concentrations in the three drainage water types were low, nevertheless, minor accumulation was observed in planters irrigated with treated GW and raw GW. This may lead to some increase of soil hydrophobicity. The COD of the drainage water of planters irrigated with raw GW was higher than the COD of the other two drainage water types. This higher leaching can contaminate surface and groundwater and promote growth of pathogen bacteria. Although raw and treated GW contained FC, they were hardly detected in the respective drainage waters. This could possibly indicate that the health risk associated with contamination of groundwater due to irrigation with GW may be minimal, however, should pathogen loads in the GW used for irrigation be high enough, they may leach from the root zone. As HPC concentrations in the drainage waters did not correlate with their concentration in the respective irrigation waters, it seems that HPC in the drainage waters is not a good indicator of the microbial quality of the drainage waters. All plants, including the ones irrigated with raw GW, did not show any signs of disease or phytotoxicity. This may be due to the fact that the GW in this study originated mainly from showers and washbasins, which are the less polluted GW streams. Irrigation with treated GW resulted in statistically significant higher biomass yield. The difference between yields of plants irrigated with freshwater and ones irrigated with raw GW was very small. No obvious reason for this difference was found, however, slightly higher nutrient levels in the treated greywater may supply an explanation for this observation. 764 Y. Alfiya et al. | Potential impacts of on-site greywater reuse ACKNOWLEDGEMENTS The study was supported by the Grand Water Research Inst. (Technion) and by IL Water Authority. REFERENCES Almeida, M. C., Butler, D. & Friedler, E. At-source domestic wastewater quality. Urban Wat. 1, 49–55. Alonso, A., Rojo, F. & Martínez, J. L. Environmental and clinical isolates of Pseudomonas aeruginosa show pathogenic and biodegradative properties irrespective of their origin. Environ. 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First received 19 July 2011; accepted in revised form 10 October 2011
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