NAME ________________________________ PERIOD ________________________________ Grades Nine Through Twelve Biology/Life Sciences Science Content Standards Standards that all students are expected to achieve in the course of their studies. Cell Biology 1. The fundamental life processes of plants and animals depend on a variety of chemical reactions that occur in specialized areas of the organism's cells. As a basis for understanding this concept: a. Students know cells are enclosed within semipermeable membranes that regulate their interaction with their surroundings. b. Students know enzymes are proteins that catalyze biochemical reactions without altering the reaction equilibrium and the activities of enzymes depend on the temperature, ionic conditions, and the pH of the surroundings. Briefly summarize the differences between the words or phrases in each of the following pairs. 1. Kinetic energy and Potential energy 2. First and second laws of thermodynamics 3. Exergonic reactions and endergonic reactions 4. Reactants and products 5. ATP and ADP; Include a discussion of the ATP Cycle 6. A reaction without an enzyme and a reaction with an enzyme An Energy profile. 7. Activation energy (EA): 8. Enzymes are proteins that act as ____________________, to speed up chemical reactions. 9. Substrate: 10. Active site of an enzyme: 11. How does an enzyme speed up a chemical reaction? 12. Diagram and discuss the “induced-fit” model scientists use to explain how enzymes might work. 13. The cellular environment affects enzyme activity. Explain how each of the following affects the rate of an enzymatic reaction. a. temperature b. pH c. increased substrate/decreased substrate 14. Define cofactors and coenzymes; What relationship do they have with enzymes? 15. Enzyme inhibitors block enzyme function. Explain each of the following. a. competitive inhibitor b. noncompetitive inhibitor c. negative feedback Membrane Structure and Function 16. Label the structures found in the plasma membrane of animal cells. d. c. e. b. g. h. f. a. 17. Explain why the plasma membrane exhibits selective permeability. Include the definition of selective permeability. 18. phospholipid bilayer: (include sketch labeling important features) 19. Scientists use the fluid-mosaic model to explain the structure and functions of the plasma membrane. Describe this model. 20. List and describe the functions of the proteins that are found in the plasma membrane. a. d. b. e. c. Movement of molecules across the plasma membrane 21. homeostasis: 22. diffusion: example: 23. passive transport: 24. concentration gradient: 25. equilibrium: 26. Substances that are able to pass through a membrane, the membrane is ________________ to it. 27. osmosis: 28. hypertonic: 29. hypotonic: 30. isotonic: 31. Directions of Osmosis Conditions a. If solute concentration in the environment is lower than in the cell b. If solute concentration in the environment is higher than in the cell c. If solute concentration in the environment is equal to that in the cell Environment solution is… Cell solution is… Water will move… 32. osmoregulation: 33. What happens to animal and plant cells when they are put into a hypotonic solution such as distilled water? Include a diagram. 34. What happens to animal and plant cells when they are put into a hypertonic solution such as seawater (high salt concentration)? Include a diagram. Osmosis is an important process that has many effects on living things. Test your understanding of osmosis by predicting in each of the following cases whether water will enter the cell (IN) or leave the cell (OUT), or whether there will be no net movement of water (NONE). Assume that the plasma membrane is permeable to water but not solutes. _____35. Cell is exposed to hypertonic solution. _____36. Cell is placed in salt solution whose concentration is greater than cell contents. _____37. Due to disease, solute concentration of body fluid outside cell is less than solute concentration of cells _____38. Cell is in isotonic solution _____39. Single-celled organism is placed in drop of pure water for examination under microscope. _____40. Cell is immersed in solution of sucrose and glucose whose individual concentrations are less than concentration of solutes in cytoplasm, but whose combined concentration is greater than concentration of solutes in cytoplasm. _____41. Solute concentration of cell is greater than solute concentration of surrounding fluid. _____42. Cell is exposed to hypotonic solution. _____43. Concentration of solutes in cytoplasm is equal to solute concentration of extracellular fluid. _____44. Cytoplasm more dilute than surrounding solution. 45. Explain the function of the contractile vacuole in a Paramecium found in freshwater. 46. If you were stranded in the ocean on a life raft and really thirsty, would you drink the seawater around you? Why/why not? Think about what you just learned about water balance in cells. 47. facilitated diffusion: which kind of molecules? 48. active transport: example: 49. Diagram and explain how an active transport system is involved with the passage of 2 different solutes across a membrane, in opposite directions. 50. Some molecules, such as food particles and waste materials, are too large to pass through the cell membrane. However, the processes called ____________________ and ____________________ enable these large molecules to enter and leave the cell without passing through the cell membrane. 51. endocytosis: 52. exocytosis: 53. pinocytosis: 54. phagocytosis: 55. receptor-mediated endocytosis: Review diffusion and the function of cell membranes by matching each of the phrases on the right with the appropriate function from the list on the left. Two questions require more than one answer. A. diffusion B. active transport C. osmosis D. phagocytosis E. passive transport F. facilitated diffusion G. pinocytosis H. receptor-mediated endocytosis I. exocytosis _____56. diffusion across a biological membrane _____57. moves solutes against concentration gradient _____58. any spread of molecules from area of higher concentration to area of lower concentration _____59. diffusion with help of transport protein _____60. three types of endocytosis _____61. engulfing of fluid in membrane vesicles _____62. diffusion of water across selectively permeable membrane, from hypotonic to hypertonic solution _____63. transport molecules need ATP to function _____64. enables cell to engulf bulk quantities of specific large molecules _____65. how oxygen and carbon dioxide enter and leave cells _____66. two types of passive transport _____67. engulfing of particle in membrane vesicle _____68. fusion of membrane-bound vesicle with membrane, and dumping of contents outside cell _____69. how a cell might capture a bacterium
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