AB SCIEX TripleTOF ™ 5600 SYSTEM
High-resolution
quant and qual
AB SCIEX TripleTOF ™ 5600 SYSTEM
AB SCIEX is a global leader in the development of life
science analytical technologies that help answer complex
scientific challenges. Scientists worldwide count on our
innovative scientific instrumentation, software, and
services to discover new drugs, advance medical science
and protect the food supply and the environment.
With a heritage of market leadership in quantitative mass
spectrometry and a passion for innovation, we provide
system solutions that maximize your productivity and the
quality of your results.
Our success relies on close partnerships with our
customers, and we are committed to bringing you the
highest level of innovation, quality, and support.
High resolution qual and quant
on one platform
The AB SCIEX TripleTOF™ 5600 System is the first accurate-mass, high-resolution LC/MS/MS
system for qualitative analysis that has the speed and sensitivity to deliver quantitation
like a high-performance triple quad.
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High sensitivity for quantitation at low
abundance levels
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SmartSpeed™ 100 Hz Acquisition collects 100
spectra/second
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Dynamic range of greater than 4 orders of magnitude
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EasyMass™ accuracy of 1ppm over 24 hours with
external calibration
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Resolution of 25,000 FWHM at low mass, m/z 100
and up to 40,000 at m/z 950, at 100 spectra/sec
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Industry-first 3 year warranty
Achieve speed, resolution
and s ensitivity simultaneously and
with no compromise.
“Among the most important
developments in mass spec
technology in years”
GERARD HOPFGARTNER, UNIV. GENEVA
One system, flexible workflows
For the first time, Pharma, Academic and Analytical Testing scientists can integrate comprehensive
qualitative exploration, rapid profiling, and high-resolution quantitation workflows on a single
platform providing faster and more accurate answers to “what is in the sample, how much is there,
and does it change?”
QUALITATIVE
ANALYSIS
QUANTITATIVE
ANALYSIS
Explore
Profile
Quantify
EXPLORE
Explore complex samples in greater depth:
Select up to 50 precursors – and generate high
resolution and high mass accuracy MS/MS spectra in
LC
High resolution MS
High resolution MS/MS
a second using powerful IDA (information-dependent
acquisition) algorithms and high-resolution, accuratemass MS and MS/MS.
Speed and resolution for definitive identification
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Select up to 50 precursors and get high resolution MS/MS in a second for fast
LC conditions
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Identify precursors with confidence with high mass accuracy in MS and MS/MS mode
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Target analyte classes with advanced IDA (information-dependent acquisition) workflows
> Multiple mass defect scanning
> Neutral loss scanning
> Exclusion / Inclusion lists / Isotope pattern matching
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Identify unknowns with robust, intelligent multiple collision energy workflows
High resolution,
accurate mass MS
PROFILE
Profile samples for quant and qual in a single run:
Quantify virtually every compound in the sample
LC
MS Quantification
High resolution,
accurate mass MS/MS
with high-resolution MS quantification and fast
chromatography; confirm compound identity with highresolution, accurate-mass MS/MS; then mine the data
for the information you need.
Quantitative and qualitative information in a single run
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Combine high-resolution MS quantification with high-resolution MS/MS ID confirmation
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Quantify every compound—even under fast LC conditions
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Re-visit data for further interrogation
High resolution,
accurate mass MS/MS
QUANTIFY
Quantify analytes with High Resolution MRM for highest
specificity and confidence: Obtain high-resolution at
high mass and low mass in the same spectrum for highresolution MRM-like quantification. LOQ’s and dynamic
range are equivalent to high-performance triple quad
instruments. Cycle times as low as 10 msec generate
Multiplexed high resolution MRM
High resolution,
accurate mass quantification
superior peak definition even with fast LC separations.
Quantification requires little optimization, and you
can choose multiple fragments per precursor from the
acquired full scan MS/MS data.
Relative & absolute quantitation with high resolution MRM (MRMHR)
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Maximize specificity using MRM-like quantitation with high resolution fragment ions
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Analyze many analytes with triple quadrupole MRM acquisition rates and collect full
scan MS/MS spectra for each precursor without additional cycle time
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Maintain fast cycle time for superior peak definition, even in fast LC separations
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Linear dynamic range of 4 orders or greater for quantitative accuracy
Inspired engineering
Beginning with the best-in-class quantitative performance of the Triple Quad™ 5500 System,
AB SCIEX researchers developed the new AcceleratorTOF™ Analyzer to deliver high resolution at the
speed and sensitivity required to maintain the precision and limits-of-quantitation associated with
MRM. The result is the breakthrough AB SCIEX TripleTOF™ 5600 System – the next generation in
Quadrupole TOF technology.
Automated Calibrant Delivery System
DuoSpray ™ source
QJet ® Ion Guide
Automated Calibration Delivery system allows easy,
MagLev turbo pumps provide ultimate robustness with silent
automated instrument calibration from a reference spray using the
running magnetic levitation technology.
DuoSpray™ source.
Software selectable dual ionization system with the
DuoSpray™ source – ESI and APCI – provides experimental flexibility
with highest performance.
> TurboIonSpray® Probe 5-3000 µL/min
> APCI Probe 50-3000 µL/min
> System also compatible with Turbo V™ source and
NanoSpray® III source with heated interface
Patented QJet® Ion Guide improves ion containment and
operates at high pressure, providing better collisional focusing
and improved ion transmission.
Next generation eQ™ electronics enable faster scan speeds
with improved sensitivity and robustness.
Patented Q0 High Pressure Cell collisionally focuses ions for
maximum transmission and sensitivity.
LINAC® collision cell increases speed of analysis and eliminates
cross-talk. True collision induced fragmentation provides reliable,
information-rich, library searchable spectra.
t 40 GHz four channel TDC and Detector provide highest
sampling speeds and maintains high resolution – even at
low mass
t 30kHz Accelerator for highest acquisition rate
t New entrance optics improves ion beam focusing post LINAC
New Accelerator TOF ™ Analyzer
improves resolution and sensitivity
t 15kV acceleration voltage for higher sensitivity and
resolution
t High transparency grids throughout for minimal ion loss
t Two-stage ion reflector compensates for energy dispersion
to maximize resolution
Performance delivered
Scientists have come to expect industry-leading performance from AB SCIEX mass spectrometers.
The TripleTOF™ 5600 System delivers it, with high-resolution, high-sensitivity data, excellent mass
accuracy stability, and fast acquisition rates. If you’re looking for uncompromised performance,
look no further than the TripleTOF™ 5600 System.
Improve productivity and specificity by acquiring information
PeakView™ Software is optimized for acquiring and processing
instead of data.
accurate mass data for multiple samples. It saves you time during
data review by enabling you to apply processing parameters across
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AutoTune provides easy, automated optimization of quadrupole
large sample sets. You can also view spectra from multiple samples
and TOF calibration and resolution as well as optimized detector
simultaneously in a single window for quick and easy qualitative
settings so that you collect the highest quality information in
review and comparison.
every run.
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Dynamic background subtraction (DBS) minimizes collection of
MS/MS on background ions to increase identification of lowlevel analytes in the presence of background noise.
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Multiple mass defect triggered IDA (Information Dependant
Acquisition) improves efficiency and reduces the need for
multiple injections to get comprehensive information. Mass
defect can be applied for specific mass ranges, and multiple
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Formula Finder Software is an enhanced elemental formula
calculator for small molecule analysis. It quickly calculates all
Neutral Loss triggered IDA provides unique specificity by
MS/MS data when available to confirm the identity of the most
triggering MS/MS when two TOF MS scans (high and low
likely candidates, while applying ‘Chemical logic’ to filter out
energy) detect a specified mass difference. The result is higher
unlikely candidates. Interactive recalibration tools based on found
efficiency IDA experiments requiring fewer runs.
common background ions or experimentally determined fragment
MS/MSALL is a powerful workflow enabled by the speed and
stepped across a mass range and high resolution, accurate mass
MS/MS is obtained at every mass step. Combined with high
resolution TOF MS data, this workflow enables the acquisition of
MS and MS/MS on all analytes.
iTRAQ® Reagent Optimization automatically adjusts the collision
energy settings specific for iTRAQ reagent labeled samples, to
ensure good ion signal is achieved for the reporter ions for high
quality quantitative results.
Auto batch calibration maintains mass accuracy during long runs
by automatically calibrating the system.
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AT MOUNT SINAI HOSPITAL
possible elemental formula for detected masses using both
MS/MS spectra are generated for all masses. A resolving Q1 is
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TONY PAWSON, SAMUEL LUNENFELD RESEARCH INSTITUTE
mass defects can be applied.
sensitivity of the Triple TOF™ 5600 System, where high quality
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“A significant leap forward...for
biomedical analysis.”
Methods Wizard enables easy creation of acquisition methods
with templates for common workflows.
accurate mass and isotope distribution. The software will use
masses ensure highest accuracy mass reporting.
Mass Accuracy (ppm)
Log Area Ratio
Log Concentration Ratio
Time (Mins)
High-resolution, high-sensitivity data with acquisition speed up to 100 spectra/sec
EasyMass™ Accuracy delivers high mass accuracy without continuous recalibration.
makes the TripleTOF 5600 System a perfect compliment to ultra-fast chromatography.
With external calibration, the mass accuracy holds rock-steady at <1ppm over 30
The system also provides 4-5 orders of magnitude of linearity to ensure accurate
minutes, and RMS=1.69 over 100 hours.
quantitation in MS and MS/MS modes.
Reserpine
R=29,950
R=34,600
R=36,500
Insulin
m/z, Da
Intensity, cps
Intensity, cps
1 s acquisition
R=43,750
100 ms acquisition
R=44,020
10 ms acquisition
R=44,000
Reserpine and fragments demonstrate high resolution at low mass, ~30,000 at 100 MS/MS
per second. Resolution of insulin (6+) is maintained at >43,000 with data accumulation
times of 1 second, 100 milliseconds, and 10 milliseconds.
Mass/Charge, amu
Applied power
Simple, clear metabolite ID, characterization, and quantitation
In-depth identification and expression analysis for Omics
and biomarker studies
From soft spot analysis to definitive metabolite ID and
characterization, to quantitation, the AB SCIEX TripleTOF™ 5600
Combine the speed of the TripleTOF™ 5600 System with high
System enables simple, clear, accurate mass metabolism workflows.
resolution and sensitivity for comprehensive identification and
quantitation workflows.
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Identify and characterize metabolites at new levels of
speed, efficiency and data quality: The TripleTOF™ 5600
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A new standard for protein identification and targeted
System is the ideal platform for rapid assessment of metabolic
quantitation: The TripleTOF™ 5600 System is capable of
stability and in vivo characterization. With the ability to perform
achieving the highest acquisition speeds while maintaining
both TOF MS and MS/MS scanning at rates suitable for fast LC,
maximum resolution and mass accuracy for unmatched protein
you can apply completely generic methodology to acquire
identification. The resolution and speed also enable MRM-like
high-quality quantitative and qualitative data simultaneously.
quantitation without significant methods development.
Quantify drug metabolites with performance equivalent to
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Comparative analysis for metabolomics studies can be
MRM on a triple quad. The TripleTOF 5600 System is the first
performed on the TripleTOF™ 5600 System with greater precision
accurate-mass, high-resolution MS system with the speed and
and confidence than ever before. Profiling of endogenous
sensitivity to deliver triple quad-like quantitation.
metabolites can be achieved with unmatched speed and
™
sensitivity to determine statical differences between samples for
the rapid determination of putative biomarkers.
Streamlined workflows for clinical research and
forensics testing
Conclusively identify and quantify low-abundance compounds in
complex samples in a single run.
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Novel workflows for Lipidomic analysis: Lipid profiling on
the TripleTOF™ 5600 System can be used to analyze every lipid
species simultaneously. With the new MS/MSALL workflow it
is possible to acquire precursor ion data at every unit mass to
The TripleTOF™ 5600 System is ideal for General Unknown
obtain high quality MS/MS data on every lipid class within
Screening (GUS) with high confidence identification and
a sample.
quantitation. High resolution and accurate mass in MS and
MS/MS modes provide superior specificity for targeted
compounds, and the data can be re-interrogated post-run to
identify unknown or unexpected components.
Multi-component food and water contaminant screening
Combine multi-target screening quantitation with non-target
screening and identification in a single run.
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High sensitivity and high resolution delivered at unmatched
speed allow MRM-like quantitation, and accurate mass MS/MS
provides the ability to detect and identify unknowns in a
single run.
You invest in our technology.
We invest in your success.
As the world leader in mass spectrometry, AB SCIEX solutions are backed by the industry’s
most extensive service and support organization. With a network of service professionals,
experienced compliance specialists, and over 150 PhD application scientists worldwide, we are
dedicated to supporting your technical needs and helping you get the most out of your
AB SCIEX systems.
AB SCIEX service professionals are recognized as the most highly qualified in the industry.
They are certified on our instrument platforms through a rigorous 4-step certification program,
with re-certification occurring every two years. This award-winning program helps to ensure
that you receive the most efficient, highest-quality, and most up-to-date service available for
AB SCIEX products and technology. Choose from flexible service plans and a variety of services
for the right level of support for your laboratory’s needs and budget.
Our customer support network is available to provide expert assistance in the use and application
of AB SCIEX products through a comprehensive range of services, including application support,
technical service, and training.
Whether you access our service and support team by phone, email, on-site visits, or through
our innovative remote monitoring technology, you can be confident that the AB SCIEX
organization will be there for you.
For more information, visit www.absciex.com
For Research Use Only. Not for use in diagnostic procedures.
© 2011 AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.
0041010-05 01/2011
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