3/4/2015 2.04 – CELL TRANSPORT: DIFFUSION AND OSMOSIS Biology 11 TRANSPORT- PASSIVE OR ACTIVE | | Passive transport-the movement of materials across a cell membrane without the expenditure of cell energy Active transport-the transport the use of cell energy to move materials across a cell membrane (against concentration gradient) PASSIVE TRANSPORT | From high to low concentration | Does not require q energy from the cell Diffusion Osmosis y Facilitated diffusion y y 1 3/4/2015 ACTIVE TRANSPORT | From low to high concentration energy from the cell | Requires Active A i transport proteins i Endocytosis y Exocytosis y y DIFFUSION & OSMOSIS KINETIC MOLECULAR THEORY | All molecules are moving. | Solid, liquid, gas | Brownian Motion– the random movement of molecules suspended in a liquid or gas. 2 3/4/2015 DIFFUSION | The NET movement of particles from an area of high concentration to an area of low concentration Q: What is a concentration gradient?? WHAT IS A CONCENTRATION GRADIENT? | Concentration Gradient the difference in concentration of solutes in a given space | Diffusion happens when substances b t move “down” “d ”a concentration gradient (from high to low) until the concentration of the two areas becomes equal. ENDING: DYNAMIC EQUILIBRIUM | At dynamic equilibrium there is no NET movement of particles. | | DYNAMIC implies movement EQUILIBRIUM implies a balance y y No longer a concentration gradient Particles move equally in all directions 3 3/4/2015 DYNAMIC EQUILIBRIUM NOTE: At D.E. particles are still moving, its just that overall, they will remain “mixed” unless acted upon by another force. (So no “net” movement overall). DIFFUSION OSMOSIS | The diffusion of WATER across a selectively- permeable membrane. http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBooktransp.html 4 3/4/2015 OSMOSIS DEMOS http://www.biologycorner.com/bio1/diffusion.html # | http://www.emc.maricopa.edu/faculty/farabee/BI OBK/BioBooktransp.html | DIFFUSION VS. OSMOSIS | Diffusion is the NET movement of particles from an area of high concentration to an area of low concentration!” | Os Osmosis os s iss just a “special spec a case oof d diffusion”. us o . It is the diffusion of water across a selectively permeable membrane. | Sometimes we refer to this movement as being from an area of HIGH water potential to an area of LOW water potential. 5 3/4/2015 BUT WHAT IS “WATER POTENTIAL”?? | Water potential is the measure of the ability of water to move (always from high to low) . | Think of osmosis as the net movement of water (remember water is also just a particle) down its concentration gradient. WHAT IS THE CAUSE OF DIFFUSION? | | Diffusion happens because of the random kinetic energy (energy of motion) of particles and the existence of a concentration gradient. All matter is made of particles and those particles are constantly in motion. WHAT AFFECTS THE RATE OF DIFFUSION IN CELLS?? | 5 Things: 1. Temperature 2. Charge 3. Diameter (Size) of particle 4. Concentration gradient (difference in concentration) 5. Surface Area of membrane 6 3/4/2015 SOLUTIONS The movement of water in and out of cells gives rise to 3 different conditions: 1. 2 2. 3. Isotonic Solutions Hypotonic Solutions Hypertonic Solutions Solute = The substance that dissolves to form a solution Solvent = A substance, usually a liquid, in which other substances are dissolved ISOTONIC solutions have equal (iso-) concentrations of dissolved substances. Solute concentrations are the same outside the cell and inside. | (Equal amounts of water movement in and out of the cell, with a net flow is zero). Ideal environment HYPOTONIC | solutions are those with less solute present and more water. – concentration of water is higher outside the cell than inside. (Water will move INTO THE CELL) 7 3/4/2015 HYPERTONIC | solutions are those in which more solute is present than water. – concentration of water is lower outside the cell than inside. ((Water will move OUT OF THE CELL)) 8
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