ISO9001 For research use only For research use only ISO9001 PRO•HuntTM His•Bind Buffer Kit 100 PRO•HuntTM His•Bind Buffer Kit 100 Product Name Qty Cat. No. Remarks Product Name Qty Cat. No. Remarks PRO·HuntTM His•Bind 1 Kit EBE-1040 Pre-made Buffers PRO·HuntTM His•Bind 1 Kit EBE-1040 Pre-made Buffers Buffer Kit 100 Buffer Kit 100 Description. Description. His•Bind Buffer Kit 100 contains all buffer components necessary PRO·HuntTM His•Bind Buffer Kit 100 contains all buffer components necessary for the purification of polyhistidine-tagged proteins. The buffer Kit provides a for the purification of polyhistidine-tagged proteins. The buffer Kit provides a convenient set of buffers optimized for purification of polyhistidine-tagged convenient set of buffers optimized for purification of polyhistidine-tagged fusion proteins. fusion proteins. Kit Components Kit Components PRO·HuntTM 5x Binding buffer 5x Binding buffer : 100 ml (1x ; 0.5 M NaCl, 5 mM imidazole, 20 mM Tris-HCl, pH 7.9) : 100 ml (1x ; 0.5 M NaCl, 5 mM imidazole, 20 mM Tris-HCl, pH 7.9) 5x Washing buffer 5x Washing buffer : 100 ml (1x ; 0.5 M NaCl, 60 mM imidazole, 20 mM Tris-HCl, pH 7.9) : 100 ml (1x ; 0.5 M NaCl, 60 mM imidazole, 20 mM Tris-HCl, pH 7.9) 5x Elution buffer 5x Elution buffer : 50 ml (1x ; 0.25 M NaCl, 0.5 M imidazole, 10 mM Tris-HCl, pH 7.9) : 50 ml (1x ; 0.25 M NaCl, 0.5 M imidazole, 10 mM Tris-HCl, pH 7.9) 5x Charging buffer 5x Charging buffer : 50 ml (1x ; 50 mM NiSO4) : 50 ml (1x ; 50 mM NiSO4) Storage Condition Storage Condition Store at 4℃, do not freeze Store at 4℃, do not freeze ㈜ 엘피스바이오텍 [302-854] 123-12 Chunglim-Dong, Seo-Gu, Taejeon, Korea ㈜ 엘피스바이오텍 [302-854] 123-12 Chunglim-Dong, Seo-Gu, Taejeon, Korea www.elpisbio.com Tel: +82-42-581-8448. Fax: +82-42-581-8449 www.elpisbio.com Tel: +82-42-581-8448. Fax: +82-42-581-8449
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