eFluor organic dyes
eFluor® organic dyes
Optimized for multicolor flow cytometry
Featuring
n
eFluor® 450
n
eFluor® 506 New
n
PerCP-eFluor® 710
n
PE-eFluor® 610
n
eFluor® 660
n
APC-eFluor® 780
The eBioscience® eFluor® organic dyes are a proprietary line of
fluorescent dyes within the eFluor® brand engineered for superior
optical performance and detection for applications using laser-based
systems, notably flow cytometry.
All eFluor® products are named for their emission wavelength, and
these organic dyes are fully compatible with other commonly used
fluorescent molecules. These features, combined with our broad
portfolio of biological content, easily enable dye selection
for optimized multicolor antibody panel design for flow cytometry.
or ®
78
0
or ®
71
0
AP
Ce
eF
F lu
lu
0
rC
P-
Pe
or ®
66
lu
eF
eF
PE
-e
F lu
6
0
or ®
50
lu
or ®
45
lu
eF
or ®
61
0
eFluor® Organic Dye Emission Spectra
100
80
60
40
20
0
300 nm
350
400
450
500
550
600
650
700
750
800
850
900
Violet laser (405 nm)
eFluor 450 (peak emission 450 nm)
The eFluor 450 organic dye was developed to provide a
high-performance fluorochrome for flow cytometry as a
competitive alternative for Pacific Blue™, Horizon™ V450
and VioBlue®. Stability data shows eFluor 450 performs
equally as bright, with a minimal loss of fluorescence,
when cells are exposed to a formaldehyde fixative
treatment for up to 24 hours.
Benefits of eFluor 450 include
n
Equal or better fluorescence intensity as compared to
several other violet-excited organic dyes
n
Stable performance when treated with aldehyde fixation
n
Broad portfolio of options to extend multicolor panel
design to the violet laser
Stability of eFluor ® 450.
Right panel: Mouse splenocytes stained with Anti-CD4 eFluor ® 450 (clone
RM4-5) and analyzed immediately (red line). The sample was then fixed in
2% formaldehyde overnight at 4 oC and analyzed again (green line).
% of Max
% of Max
Fluorescence intensity comparison.
Left panel: Human PBMCs stained with Anti-CD45RA (clone HI100)
conjugated to eFluor ® 450 (purple line) or Pacific Blue™ (blue dotted line).
CD45RA (clone H100)
CD4 (clone RM4-5) eFluor® 450
eFluor 506 (peak emission 506 nm)
The newest member of the eFluor® family of conjugates,
eFluor 506, adds versatility to multicolor panels designed
for use with violet laser-equipped flow cytometers.
Like other eFluor reagents, it is engineered for superior
optical performance and compatibility with eBioscience®
fluorochrome conjugates, buffers, and fixatives.
Benefits of eFluor 506 include
n
Direct alternative for AmCyan and Horizon V500
n
Similar or better fluorescence intensity than other
500 nm-emitting organic dyes
n
Compatible with intracellular staining protocols for
CD4 (RM4-5)
2
Fluorescence intensity comparison.
Left panel: Mouse splenocytes were stained with CD4 (clone RM4-5)
conjugated to eFluor ® 506 (purple line) or to Horizon™ V500
(blue histogram), using the same concentration of antibody.
% of Max
% of Max
cytokine or transcription factor analysis
Stability of eFluor ® 506.
Right panel: Mouse splenocytes stained with Anti-CD4 (clone RM4-5)
and either left unfixed (blue line), or were fixed for 30 minutes in Foxp3/
Transcription Factor buffer set (purple line) or 30 minutes in Intracellular
(IC) Fixation buffer followed by methanol (orange line).
CD4 (RM4-5) eFluor® 506
Blue laser (488 nm)
PerCP-eFluor 710 (peak emission 710 nm)
The PerCP-eFluor 710 tandem dye was developed as an
optimal choice for the FL3 channel on a blue laser line.
It is consistently two to three times brighter than PerCPCyanine5.5 when evaluated in clone-to-clone comparison
tests on identical samples. Although PerCP-eFluor 710
is slightly red-shifted in its emission compared to PerCPCyanine5.5 (710 nm vs. 685 nm), it uses the same filter sets.
n
Greater mean fluorescence intensity (MFI) as compared
n
No need for compensation out of the PE detector
n
Stable performance when treated with formaldehyde
to PerCP-Cyanine5.5
fixation or exposed to ambient light
Staining performance compared to PerCP-Cyanine5.5.
Left panel: Direct comparison of mouse splenocytes stained with
Anti-mouse CD4 (clone RM4-5) conjugated to either PerCP-eFluor ® 710
(red line) or PerCP-Cyanine5.5 (pink line).
% of Max
% of Max
CD4 (clone RM4-5)
Benefits of PerCP-eFluor 710 include
Effects of light exposure or fixation on PerCP-eFluor ® 710.
Right panel: Human PBMCs were stained with Anti-CD4 PerCP-eFluor ®
710 (clone SK3) and analyzed immediately (red line). The sample was then
divided and either exposed to ambient light for six hours (blue line) or fixed
in 2% formaldehyde overnight at 4 oC (green line) and analyzed again.
CD4 (clone SK3)
Yellow-green laser (561 nm)
Benefits of PerCP-eFluor 610 include
PE-eFluor 610 (peak emission 610 nm)
CD3
n
Brighter than PE-Texas Red
n
Reduced compensation out of other far-red channels
n
Stable performance when treated with formaldehyde
fixation or exposed to ambient light
PE-eFluor ® 610 is brighter than PE-Texas Red®.
Left panel: Normal human peripheral blood cells were stained with CD3
conjugated to PE-eFluor ® 610 (dark orange line) or PE-Texas Red (light
orange line).
% of Max
% of Max
PE-eFluor 610 is a high-performance tandem fluorophore for
use in multicolor flow cytometry. It is designed for use with
flow cytometers equipped with blue, green, or yellow-green
laser lines (488, 532, or 561 nm) and has a peak emission of
607 nm that can be detected using typical filters for
PE-Texas® Red, such as a 610/20 bandpass filter.
Photostability of PE-eFluor ® 610.
Right panel: Normal human peripheral blood cells were stained with
Anti-CD3 PE-eFluor® 610 (clone UCHT1). Cells were then washed and incubated
for six hours at 4oC in the dark (red line), at room temperature in ambient light
(blue line), or at room temperature on the windowsill (orange line).
CD3 PE-eFluor® 610
3
Red laser (633 nm)
eFluor 660 (peak emission 668 nm)
eFluor 660 is configured as an alternative to Alexa Fluor™
647 or APC. The spectral properties make it an ideal
fluorophore that can be used in any flow cytometry
panel incorporating the red laser.
Benefits of eFluor 660 include
n
Direct alternative for APC and Alexa Fluor 647
fluorochromes
n
Stable performance when treated with
formaldehyde fixation
% of Max
Effects of fixation on eFluor ® 660.
Mouse splenocytes stained with Anti-CD4 eFluor ® 660 (clone GK1.5) and
analyzed immediately (dotted red line). The sample was then fixed in 2%
formaldehyde overnight at 4 oC (blue line) and analyzed again.
CD4 (clone GK1.5)
APC-eFluor 780 (peak emission 780 nm)
APC-eFluor 780 has similar spectral properties to APC-H7,
APC- Cyanine7 and APC-Alexa Fluor™ 750. It can replace
any of these tandem dyes when designing a multicolor
staining panel. The APC-eFluor 780 tandem dye is easily
compensated when used with APC.
Benefits of APC-eFluor 780 include
n
Equal or better sensitivity than APC-H7 tandem
n
Stable performance when treated with formaldehyde
conjugates
fixation or exposed to ambient light
n
Uses same filter sets as existing comparative
fluorochromes
% of Max
% of Max
Comparison with APC-H7.
Left panel: Mouse splenocytes stained with Anti-CD8 (clone 53-6.7)
conjugated to APC-eFluor ® 780 (blue line) or APC-H7 (red line).
CD8 (clone 53-6.7)
4
CD8 (clone RPA-T8) APC-eFluor® 780
Stability of APC-eFluor 780.
Right panel: Human peripheral blood mononuclear cells (PBMCs) were
stained with Anti-CD8 APC-eFluor ® 780 (clone RPA-T8) and analyzed
immediately (red line). The sample was then divided and either exposed to
ambient light for six hours (blue line) or fixed in 2% formaldehyde for
30 minutes at room temperature (green line) and analyzed again.
eFluor product listing
Violet laser
Human (continued)
Clone
Cat. No.
AHR
AKT (pS473)
alpha beta TCR
Basophil Marker
BATF
Bcl-6
BTK/ITK (pY551/Y551)
beta-Catenin
CCL4 (MIP-1b)
CCL5 (RANTES)
CCL7 (MCP-3)
CCL8 (MCP-2)
CD1a
CD1c
CD1d
CD2
CD3
CD3
CD3
CD4
CD4
CD4
CD5
CD7
CD7
CD8
CD8
CD8a
CD10
CD11b
CD11b
CD11c
CD11c
CD13
CD14
CD15
CD15
CD16
CD19
CD19
CD19
CD20
CD21
CD22
CD23
CD24
CD25
CD27
CD28
CD29
CD31 (PECAM-1)
CD32
CD33
CD34
CD36
CD38
CD38
CD39
CD40
CD41a
CD42a
CD44
CD45
CD45
CD45R (B220)
CD45RA
CD45RO
CD46
CD47
CD49a (Integrin alpha 1)
FF3399
SDRNR
IP26
2D7
MBM7C7
BCL-UP
M4G3LN
15B8
FL34Z3L
VL1
OLGMASCE
DWZEE
HI149
L161
51.1
RPA-2.10
SK7
OKT3
UCHT1
SK3 (SK-3)
OKT4 (OKT-4)
RPA-T4
UCHT2
4H9
eBio124-1D1 (124-1D1)
OKT8 (OKT-8)
SK1
RPA-T8
SN5c
CBRM1/5
ICRF44
3.9
BU15
WM-15 (WM15)
61D3
MMA
HI98
eBioCB16 (CB16)
J3-129
SJ25C1
HIB19
2H7
HB5
eBio4KB128 (4KB128)
EBVCS2
eBioSN3 (SN3 A5-2H10)
BC96
O323
CD28.2
TS2/16
WM-59 (WM59)
6C4 (CD32)
P67.6
4H11
eBioNL07 (NL07)
HB7
HIT2
eBioA1 (A1)
5C3
HIP8
GR-P
IM7
HI30
2D1
RA3-6B2
HI100
UCHL1
8E2
B6H12
TS2/7
9854
9715
9986
9748
9860
9880
9015
2567
7540
9905
7077
9789
0019
0015
0016
0029
0036
0037
0038
0047
0048
0049
0059
0078
0079
0086
0087
0088
0108
0113
0118
0116
0128
0138
0149
0158
0159
0168
0197
0198
0199
0209
0219
0229
0238
0247
0259
0279
0289
0299
0319
0329
0337
0349
0369
0388
0389
0399
0409
0419
0428
0441
0459
9459
0452
0458
0457
0469
0479
9490
Blue laser
eFluor® 450 eFluor® 506
Blue/Green/
Yellowgreen laser
Red laser
PerCPPEAPCeFluor® 660
eFluor® 710 eFluor®610
eFluor® 780
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n
5
Violet laser
6
Human (continued)
Clone
Cat. No.
CD49f (Integrin alpha 6)
CD54 (ICAM-1)
CD56 (NCAM)
CD56 (NCAM)
CD57
CD62L (L-selectin)
CD62P (P-Selectin)
CD63
CD64 (Fc gamma Receptor 1)
CD68
CD69
CD70
CD72
CD73
CD75
CD79a
CD80 (B7-1)
CD81
CD83
CD85d (ILT4)
CD86 (B7-2)
CD87 (UPAR)
CD90 (Thy-1)
CD91
CD95 (APO-1/Fas)
CD96 (TACTILE)
CD103 (Integrin alpha E)
CD104 (Integrin beta 4)
CD105 (Endoglin)
CD107a (LAMP-1)
CD107b (LAMP-2)
CD114
CD117 (c-kit)
CD122
CD123
CD126
CD127
CD133
CD137 (4-1BB)
CD146
CD152 (CTLA-4)
CD154 (CD40 Ligand)
CD155
CD158f
CD160
CD161
CD162 (PSGL-1)
CD163
CD166 (ALCAM)
CD169 (Siglec-1)
CD172a (SIRP alpha)
CD177
CD182 (CXCR2)
CD183 (CXCR3)
CD184 (CXCR4)
CD185 (CXCR5)
CD194 (CCR4)
CD195 (CCR5)
CD196 (CCR6)
CD197 (CCR7)
CD199 (CCR9)
CD200
CD200 Receptor
CD206 (MMR)
CD217 (IL-17 Receptor A)
CD223 (Lag-3)
CD235a (Glycophorin A)
CD235a (Glycophorin A)
CD243 (ABCB1)
CD247
CD258 (LIGHT)
eBioGoH3 (GoH3)
HA58
TULY56
CMSSB
TB01 (TBO1)
DREG-56 (DREG56)
Psel.KO2.3
H5C6
10.1
eBioY1/82A (Y1/82A)
FN50
113-16
J4-117
AD2
LN-1
HM47
2D10.4
1D6-CD81
HB15e
42D1
IT2.2
VIM5
eBio5E10 (5E10)
A2MR-a2
DX2
NK92.39
Ber-ACT8
439-9B
SN6
eBioH4A3
eBioH4B4 (H4B4)
LMM741
104D2
TU27
6H6
47.7G7.1F2
eBioRDR5
TMP4
4B4 (4B4-1)
P1H12
14D3
24-31
2H7CD155
HP-MA4
BY55
HP-3G10
FLEG
eBioGHI/61 (GHI/61)
3A6
7-239
15-414
MEM-166 (MEM166)
ebio5E8-C7-F10 (5E8-C7-F10)
CEW33D
12G5
MU5UBEE
D8SEE
NP-6G4
R6H1
3D12
BBC3M4
OX104
OX108
19.2
424LTS
3DS223H
6A7M
HIR2 (also GA-R2)
UIC2
6B10.2
7-3 (7)
0495
0549
0566
0567
0577
0629
0626
0639
0649
0689
0699
0709
0729
0739
0759
0792
0809
0819
0839
5149
0869
3879
0909
0919
0959
0969
1037
1049
1057
1079
1078
1149
1178
1228
1239
1269
1278
1338
1379
1469
1529
1548
1550
1589
1609
1619
1629
1639
1668
1699
1729
1779
1829
1839
9999
9185
1949
1956
1969
1979
1999
9200
9201
2069
7917
2239
9884
9987
2439
2479
2589
Blue laser
eFluor® 450 eFluor® 506
n
Blue/Green/
Yellowgreen laser
Red laser
PerCPPEAPCeFluor® 660
eFluor® 710 eFluor®610
eFluor® 780
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n
n
n
n
n
n
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n
Violet laser
Human (continued)
Clone
Cat. No.
CD273 (B7-DC)
CD274 (B7-H1)
CD276 (B7-H3)
CD278 (ICOS)
CD279 (PD-1)
CD279 (PD-1)
CD294
CD300c
CD300f (IREM-1)
CD303a
CD304
CD307d
CD307e
CD314 (NKG2D)
CD319 (CRACC)
CD324 (E-cadherin)
CD325 (N-cadherin)
CD326 (EpCAM)
CD335 (NKp46)
CD336 (NKp44)
CD337 (NKp30)
CD354
CD357 (AITR/GITR)
CD360
CD363
CD366 (TIM3)
CD368 (Clec6)
CD369 (Clec7A, Dectin-1)
c-ErbB3/HER3
c-Maf
Cutaneous Lymphocyte Antigen
CX3CR1
CXCL1 (GRO alpha)
CXCL10 (IP-10)
EBI3 (IL-27 subunit)
EGFR
Egr1
Eomes
ERK 1, ERK 2
Fc epsilon Receptor 1 alpha (FceRI)
Foxp3
Foxp3
Foxp3
Galectin-9
Gata-3
GARP
G-CSF
Glycoprotein VI
Granzyme A
Granzyme K
Granzyme M
phospho-H2AX (S139)
Helios
HLA-A2
HLA-ABC
HLA-DR
HLA-DR
HLA-G
IDO
IFN gamma
Ig kappa light chain
IgD
IgM
IL-2
IL-6
IL-8
IL-9
IL-9
IL-10
IL-12
IL-12/IL-23
IL-12/IL-23
MIH18
MIH1
7-517
ISA-3
eBioJ105 (J105)
MIH4
BM16
TX45
UP-D1
201A
TNKUSOHA
413D12
509F6
1D11
162
DECMA-1
8C11
1B7
9E2
44.189
AF29-4D12
Lndnb3r
eBioAITR
2SX21R
SW4GYPP
F38-2E2
9B9
15E2
SGP1
sym0F1
HECA-452
2A9-1
KTYFLF
4NY8UN
ebic6
me1B3
HEGR1DS
WD1928
MILAN8R
AER-37 (CRA1)
150D/E4
236A/E7
PCH101
9M1-3
TWAJ
G14D9
8F5CSF
HY101
CB9
G3H69
4B2G4
CR55T33
22F6
BB7.2
W6/32
L243
LN3
87G
eyedio
4S.B3
TB28-2
IA6-2
SA-DA4
MQ1-17H12
MQ2-13A5
8CH
MH9A4
MH9D1
JES3-9D7
SNKY35
C8.6
eBioHP40 (HP40, HP-40)
5888
5983
2769
9948
2799
9969
2949
3006
3008
9818
3049
3079
3078
5878
2229
3249
3259
9326
3359
3369
3379
3549
5875
3601
3639
3109
9113
9856
6555
9855
9857
6099
7515
9744
7358
9509
9851
4877
9109
5899
4774
4777
4776
9116
9966
9882
7351
9813
9177
8897
9774
9865
9883
9876
9983
9952
9956
9957
9477
7319
9970
9868
9998
7029
7069
8088
7097
7098
7108
7359
7129
7235
Blue laser
eFluor® 450 eFluor® 506
Blue/Green/
Yellowgreen laser
Red laser
PerCPPEAPCeFluor® 660
eFluor® 710 eFluor®610
eFluor® 780
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7
Violet laser
8
Human (continued)
Clone
Cat. No.
IL-17A
IL-17A
IL-17AF
IL-17F
IL-17FF
IL-21
IL-22
IL-23 p19
IL-27 p28
IL-31
IRF4
IRF5
IRF8
Ki-67
KLRG1
LAMP5
LAP
LCK (pY505)
Mature macrophage marker
Myeloperoxidase (MPO)
NF-kappa B p65 (pS529)
Osteopontin
p38 MAPK (pT180/Y182)
PARP
Perforin
Phosphotyrosine
Podoplanin
Progesterone Receptor
S100A8
S6 Ribosomal Protein
Semaphorin 4A
SLAM Associated Protein (SAP)
Sox2
Src (pY418)
SSEA-1
SSEA-4
STAT1 (pY701)
STAT3 (pY705)
STAT5 (pY694)
STAT6 (pY641)
Survivin (BIRC5)
T-bet
gamma delta TCR
TIGIT
TL1A
TNF alpha
mTOR (pS2448)
TRA-1-60 (podocalyxin)
TSLP Receptor
V alpha 24 J alpha 18 TCR
V beta 8 TCR
ZAP-70/SYK (pY319/Y352)
eBio64CAP17
eBio64DEC17
20LJS09
SHLR17
WU24A4P
eBio3A3-N2 (3A3-N2)
22URTI
23dcdp
3D1p28
31SNEZE
3E4
ALYSCLN
V3GYWCH
20Raj1
13F12F2
34.2
FNLAP
SRRCHA
eBio25F9 (25F9)
MPO455-8E6
B33B4WP
2F10
4NIT4KK
HLNC4
dG9 (delta G9)
pY20
NZ-1.3
KMC912
CF-145
cupk43k
5E3
XLP-1D12
Btjce
SC1T2M3
eBioMC-480 (MC-480)
eBioMC-813-70 (MC-813-70)
KIKSI0803
LUVNKLA
SRBCZX
CHI2S4N
STLALYV
eBio4B10 (4B10, 4-B10)
B1.1
MBSA43
Tandys1a
MAb11
MRRBY
TRA-1-60
eBio1A6 (1A6)
6B11
JR-2
n3kobu5
7178
7179
9179
7169
9178
7219
7229
7823
8277
9319
9858
9698
9852
5699
5893
9778
9829
9076
0115
1299
9863
9096
9078
6668
9994
5001
9381
9764
9745
9007
9753
9787
9811
9034
8813
8843
9008
9033
9010
9013
9176
5825
9959
9500
7911
7349
9718
8863
5499
5806
9890
9006
Blue laser
eFluor® 450 eFluor® 506
Blue/Green/
Yellowgreen laser
Red laser
PerCPPEAPCeFluor® 660
eFluor® 710 eFluor®610
eFluor® 780
n
n
n
n
n
n
n
n
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n
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n
n
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n
n
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n
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n
n
n
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n
n
n
n
n
n
n
n
n
n
n
n
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n
n
n
n
n
n
n
n
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n
n
n
n
n
n
n
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n
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n
n
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n
Violet laser
Mouse
Human (continued)
Clone
Cat. No.
AHR
Aiolos
AKT (pS473)
Bcl-2
Bcl-6
BP-1
BTK/ITK (pY551/Y551)
beta-Catenin
CCL3 (MIP-1 alpha)
CD1d
CD3
CD3e
CD3e
CD4
CD4
CD5
CD8a
CD8b
CD9
CD11a
CD11b
CD11c
CD14
CD16/CD32
CD19
CD21/CD35
CD21/CD35
CD23
CD24
CD25
CD25
CD25
CD27
CD29
CD31 (PECAM-1)
CD34
CD38
CD39
CD40
CD41
CD42d
CD44
CD45
CD45.1
CD45.2
CD45R (B220)
CD47
CD48
CD49b (Integrin alpha 2)
CD49d (Integrin alpha 4)
CD49f (Integrin alpha 6)
CD62L (L-selectin)
CD62P (P-Selectin)
CD63
CD68
CD69
CD70
CD71 (Transferrin Receptor)
CD73
CD79
CD80 (B7-1)
CD83
CD86 (B7-2)
CD90.1 (Thy-1.1)
CD90.2 (Thy-1.2)
CD90.2 (Thy-1.2)
CD93 (AA4.1)
CD94
CD95 (APO-1/Fas)
CD102 (ICAM-2)
CD103 (Integrin alpha E)
CD105 (Endoglin)
4MEJJ
8B2
SDRNR
10C4
BCL-DWN
6C3
M4G3LN
15B8
DNT3CC
1B1
17A2
145-2C11
eBio500A2 (500A2)
GK1.5
RM4-5
53-7.3
53-6.7
eBioH35-17.2 (H35-17.2)
eBioKMC8 (KMC8)
M17/4
M1/70
N418
Sa2-8
93
eBio1D3 (1D3)
eBio8D9
eBio4E3 (4E3)
B3B4
M1/69
PC61.5
eBio7D4 (7D4)
eBio3C7 (3C7)
LG.7F9
eBioHMb1-1 (HMb1-1)
390
RAM34
90
24DMS1
1C10
eBioMWReg30 (MWReg30)
1C2
IM7
30-F11
A20
104
RA3-6B2
miap301
HM48-1
DX5
R1-2
eBioGoH3 (GoH3)
MEL-14
Psel.KO2.3
NVG-2
FA-11
H1.2F3
FR70
R17217 (RI7 217.1.4)
eBioTY/11.8 (TY/11.8)
24C2.5
16-10A1
Michel-17 (Michel17)
GL1
HIS51
53-2.1
30-H12
AA4.1
18d3
15A7
3C4 (mIC2/4)
2E7
MJ7/18
5925
5789
9715
6992
5453
5891
9015
2567
7532
0011
0032
0031
0033
0041
0042
0051
0081
0083
0091
0111
0112
0114
0141
0161
0193
0211
0212
0232
0242
0251
0252
0253
0271
0291
0311
0341
0381
0391
0401
0411
0421
0441
0451
0453
0454
0452
0471
0481
5971
0492
0495
0621
0626
0631
0681
0691
0701
0711
0731
0791
0801
0831
0862
0900
0902
0903
5892
0941
0951
1021
1031
1051
Blue laser
eFluor® 450 eFluor® 506
Blue/Green/
Yellowgreen laser
Red laser
PerCPPEAPCeFluor® 660
eFluor® 710 eFluor®610
eFluor® 780
n
n
n
n
n
n
n
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n
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n
n
9
Violet laser
10
Human(continued)
(continued)
Mouse
Clone
Cat. No.
CD106 (VCAM-1)
CD107a (LAMP-1)
CD107b (LAMP-2)
CD115 (c-fms)
CD117 (c-kit)
CD117 (c-kit)
CD122
CD127
CD127
CD133 (Prominin-1)
CD134 (OX40)
CD135 (Flt3)
CD137 (4-1BB)
CD137 Ligand (4-1BB Ligand)
CD144 (VE-Cadherin)
CD146
CD150
CD152 (CTLA-4)
CD154 (CD40 Ligand)
CD160
CD169 (Siglec-1)
CD170 (Siglec F)
CD172a (SIRP alpha)
CD178 (Fas Ligand)
CD184 (CXCR4)
CD185 (CXCR5)
CD195 (CCR5)
CD196 (CCR6)
CD197 (CCR7)
CD199 (CCR9)
CD200
CD200 Receptor
CD201 (EPCR)
CD205
CD207 (Langerin)
CD209 (DC-SIGN), CD209a
CD215 (IL-15 Receptor alpha)
CD218a (IL-18 Receptor alpha)
CD223 (Lag-3)
CD244.1
CD252 (OX40 Ligand)
CD272 (BTLA)
CD273 (B7-DC)
CD274 (B7-H1)
CD275 (B7-H2)
CD278 (ICOS)
CD279 (PD-1)
CD279 (PD-1)
CD281 (TLR1)
CD282 (TLR2)
CD301b (MGL2)
CD304
CD314 (NKG2D)
CD317 (BST2/PDCA-1)
CD324 (E-cadherin)
CD326 (EpCAM)
CD335 (NKp46)
CD354
CD357 (GITR)
CD366 (TIM3)
CD369 (Clec7A, Dectin-1)
CD370 (Clec9A)
c-Maf
c-Rel
CXCL9 (MIG)
Dendritic Cell Marker (33D1)
DO11.10 TCR
Endomucin
Eomes
Eos
ERK 1, ERK 2
F4/80 Antigen
429
eBio1D4B (1D4B)
eBioABL-93
AFS98
2B8
ACK2
TM-b1 (TM-beta1)
A7R34
eBioSB/199 (SB/199)
13A4
OX-86
A2F10
17B5
TKS-1
eBioBV13 (BV13)
P1H12
mShad150
UC10-4B9
MR1
eBioCNX46-3 (CNX46-3)
SER-4
1RNM44N
P84
MFL3
2B11
SPRCL5
HM-CCR5 (7A4)
sirx6
4B12
eBioCW-1.2 (CW-1.2)
OX90
OX110
eBio1560 (1560)
205yekta
eBioRMUL.2
MMD3
DNT15Ra
P3TUNYA
eBioC9B7W (C9B7W)
C9.1
RM134L
6F7
122
MIH5
HK5.3
15F9
RMP1-30
J43
eBioTR23 (TR23)
6C2
11A10-B7
3DS304M
CX5
eBio927
DECMA-1
G8.8
29A1.4
TR3MBL1
DTA-1
8B.2C12
bg1fpj
42D2
sym0F1
1RELAH5
MIG-2F5.5
33D1
KJ1-26 (KJ126, KJI-26, KJI26)
eBioV.7C7 (V.7C7)
Dan11mag
ESB7C2
MILAN8R
BM8
1061
1071
1072
1152
1171
1172
1222
1271
1273
1331
1341
1351
1371
5901
1441
1469
1502
1522
1541
1601
5755
1702
1721
5911
9991
7185
1951
7196
1971
1991
5200
5201
2012
2051
2073
2094
7149
5183
2231
2440
5905
5950
9972
5982
5985
5890
9981
9985
9011
9021
3011
3041
5882
3172
3249
5791
3351
3541
5874
5871
5859
5975
9855
6111
3009
5884
5808
5851
4875
5758
9109
4801
Blue laser
eFluor® 450 eFluor® 506
Blue/Green/
Yellowgreen laser
Red laser
PerCPPEAPCeFluor® 660
eFluor® 710 eFluor®610
eFluor® 780
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
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n
n
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n
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n
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n
n
n
n
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n
n
n
n
n
n
n
n
n
n
n
n
n
n
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n
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n
n
n
n
n
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n
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n
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n
n
n
n
n
Violet laser
Human(continued)
(continued)
Mouse
Clone
Cat. No.
Fc epsilon Receptor 1 alpha (FceRI)
Foxp3
Galectin-3
GARP
Gata-3
G-CSF
GL7 (T and B Cell Activation Marker)
GP49 receptor
Gpnmb
Granzyme A
Granzyme B
phospho-H2AX (S139)
Helios
I kappa B zeta
IDO
IFN gamma
IgD
IgG
IgG1
IgG2a
IgM
IgM
IL-1 beta
IL-2
IL-4
IL-6
IL-9
IL-12 p35
IL-12/IL-23 p40
IL-13
IL-17A
IL-17AF
IL-17F
IL-21
IL-21
IL-22
IL-23
IL-25R (IL-17RB)
IL-33R (ST2)
IL-33R (ST2)
Integrin alpha 4 beta 7 (LPAM-1)
IRF4
IRF8
Jagged 2
Ki-67
KLRG1
LAP
LCK (pY505)
Ly-49D
Ly-49E/F
Ly-49G2
Ly-6A/E (Sca-1)
Ly-6C
Ly-6D
Ly-6G (Gr-1)
Ly-6G (Gr-1)
Lyve-1
MHC Class I (H-2Dd)
MHC Class I (H-2Kd)
MHC Class I (H-2Kd)
MHC Class I (H-2Kd/H-2Dd)
MHC Class I (H-2Kk)
MHC Class II (I-A/I-E)
MHC Class II I-Ab
mTOR (pS2448)
NK1.1
NKG2A/C/E
NKG2AB6
NOS2
Nur77
OVA257-264 (SIINFEKL)
PD-1H
MAR-1
FJK-16s
eBioM3/38 (M3/38)
YGIC86
TWAJ
9B4CSF
GL-7 (GL7)
H1.1
CTSREVL
GzA-3G8.5
NGZB
CR55T33
22F6
LK2NAP
mIDO-48
XMG1.2
11-26c (11-26)
polyclonal
M1-14D12
m2a-15F8
II/41
eB121-15F9
NJTEN3
JES6-5H4
11B11
MP5-20F3
RM9A4
4D10p35
C17.8
eBio13A
eBio17B7
B8KN8R
eBio18F10
FFA21
mhalx21
1H8PWSR
fc23cpg
MUNC33
RMST2-33
RMST2-2
DATK32 (DATK-32)
3E4
V3GYWCH
HMJ2-1
SolA15
2F1
TW7-16B4
SRRCHA
eBio4E5 (4E5)
CM4
eBio4D11 (4D11)
D7
HK1.4
49-H4
1A8-Ly6g
RB6-8C5
ALY7
28-14-8
AF6-88.5.5.3
SF1-1.1.1
34-1-2S
AF3-12.1.3
M5/114.15.2
AF6-120.1
MRRBY
PK136
20d5
16a11
CXNFT
12.14
eBio25-D1.16 (25-D1.16)
MH5A
5898
5773
5301
9891
9966
7353
5902
5784
5708
5831
8898
9865
9883
6801
9473
7311
5993
4010
4015
4210
5790
5890
7114
7021
7041
7061
8091
7352
7123
7133
7177
9171
7471
7211
7213
7221
7023
7361
9333
9335
5887
9858
9852
3392
5698
5893
9821
9076
5782
5848
5781
5981
5932
5974
9668
5931
0443
5999
5958
5957
5998
5940
5321
5320
9718
5941
5896
5897
5920
5965
5743
5919
Blue laser
eFluor® 450 eFluor® 506
n
n
Blue/Green/
Yellowgreen laser
Red laser
PerCPPEAPCeFluor® 660
eFluor® 710 eFluor®610
eFluor® 780
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
n
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n
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n
n
n
n
n
n
n
n
n
n
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n
n
n
n
n
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n
n
n
n
n
n
n
n
n
n
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n
n
11
Violet laser
Human (continued)
(continued)
Mouse
Clone
Cat. No.
Phosphotyrosine
Podoplanin
ROR gamma (t)
S6 Ribosomal Protein
Semaphorin 4A
Siglec G
Siglec H
Sox2
Src (pY418)
SSEA-1
STAT3 (pY705)
STAT5 (pY694)
STAT6 (pY641)
T-bet
TCR beta
gamma delta TCR
TER-119
Themis
TIGIT
TIM4
TL1A
TNF alpha
TOX
V alpha 2 TCR
V alpha 3.2 TCR
V alpha 11 TCR
V beta 5.1/5.2 TCR
V beta 6 TCR
V beta 8.1, 8.2 TCR
V beta 9 TCR
V beta 11 TCR
V beta 12 TCR
V beta 13 TCR
V delta 4 TCR
V gamma 2 TCR
ZAP-70/SYK (pY319/Y52)
3G11 Sialoganglioside Antigen
pY20
eBio8.1.1 (8.1.1)
B2D
cupk43k
5E3
SH2.1
eBio440c
Btjce
SC1T2M3
eBioMC-480 (MC-480)
LUVNKLA
SRBCZX
CHI2S4N
eBio4B10 (4B10, 4-B10)
H57-597
eBioGL3 (GL-3, GL3)
TER-119
1TMYS
GIGD7
54 (RMT4-54)
Tandys1a
MP6-XT22
TXRX10
B20.1
RR3-16
RR8-1
MR9-4
RR4-7
KJ16-133 (KJ-16, KJI6, KJ-I6)
MR10-2
RR3-15
MR11-1
MR12-3
GL2
UC3-10A6
n3kobu5
eBio3G11 (SM3G11, 3G11)
5001
5381
6981
9007
9753
5833
0333
9811
9034
8813
9033
9010
9013
5825
5961
5711
5921
5918
9501
5866
7911
7321
6502
5812
5799
5800
5796
5795
5813
5823
5827
5798
5797
5702
5828
9006
5443
Affymetrix, Inc: (US) +1-888-362-2447, +1-408-731-5000
USB Products: (US) +1-800-321-9322, +1-216-765-5000
eFluor® 450 eFluor® 506
n
Blue/Green/
Yellowgreen laser
Red laser
PerCPPEAPCeFluor® 660
eFluor® 710 eFluor®610
eFluor® 780
n
n
n
n
n
n
n
n
n
n
n
n
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(EU) +44-(0)1628-552550
eBioscience Products: (US) +1-888-999-1371, +1-858-642-2058
Blue laser
n
n
n
n
n
n
n
(JP) +81-(0)3-6430-4020
(EU) +43-1-796-40-40-305
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P/N FC07353 Rev 2 eFluor Organic Dyes PLF 1216