PondProtect®
Aquaculture Pond Treatment
Ammunition Against Ammonia and Nitrite
The Aquaculture Pond Environment
The importance of a good pond environment for shrimp
culture cannot be over-emphasized. Stress caused by
poor environmental
Managing the pond
conditions negatively
environment is a
affects the growth,
critical success factor
feeding, disease
for effective and profitable
resistance and ultimately
shrimp farming
the yield of the shrimp.
The pond environment
is a complex web of inter-linking processes. Maintaining the
balance of critical parameters is a fundamental requirement
for successful shrimp culture. Two critical parameters are
ammonia and nitrite.
Ammonia Problem in Shrimp Aquaculture
Ammonia and nitrite are generated in the aquaculture
pond by the decomposition of organic matter like fecal
waste of the shrimp, excess feed, dead plankton and other
nitrogenous matter under aerobic and anaerobic conditions.
High temperature, high pH values and absence of oxygen
in the pond water also augment ammonia generation.
Under healthy pond
conditions, ammonia is
converted to nitrite and
finally harmless nitrate
and nitrogen by the action
of naturally-occurring
bacteria in the pond
system. However, these
bacteria are slow-growing, Anaerobic pond bottom soil is indicative
slow-acting and often out- of a pond with a serious ammonia
spike.
competed by other species.
Hence, the process of nitrification is very slow and in the event
of anaerobic conditions doesn’t reach completion causing
ammonia and nitrite to build up in the pond.
Ammonia build-ups create toxic conditions for shrimp:
increase blood pH; reduce oxygen conductivity in the blood;
reduce oxygen content of the blood, affect the gills of the
shrimp, and increase mortality rates.
Most farmers today resort to physical and chemical treatments
to control ammonia and nitrite. Such treatments are often
inadequate, ineffective or short-lived. Ammonia and nitrite
control is a major cause of concern for the shrimp farmer.
Remedy from Novozymes Biologicals - Nitrifying Bacteria
Microbial technology from Novozymes Biologicals, world
leaders in microbial ecology, can provide a solution to these
problems. Novozymes’ PondProtect is a consortium of
non-pathogenic, nitrifying bacteria capable of effective
removal of ammonia and nitrite waste from shrimp ponds.
It is a combination of two specific nitrifying bacteria that
work together to convert harmful ammonia first to nitrite
and then to harmless nitrate. The more ammonia or nitrite
builds up, the more the consortium grows and is activated
to handle the ammonia/nitrite buildup.
This bacterial consortium supplements the naturally-occurring
nitrifying bacteria in the pond and accelerates the process
of nitrification.
Technical Benefits
Nitrite (NO2 ppm)
Ammonia (NH3 ppm)
Ammonia and Nitrite Reduction
Treatment
begins
Treatment
begins
PondProtect has shown in successful trials to drastically reduce ammonia and nitrite levels in shrimp ponds
The graphs show the toxic build up of ammonia and nitrites in the non-treated (control) pond with advancing culture days.
However, in ponds treated with PondProtect, ammonia levels and nitrite levels remained relatively low. The NH3 and especially
NO2 spikes in the non-treated ponds were serious enough to cause mortality of the culture and force early harvesting. Treated
ponds had longer culture periods than non-treated ponds.
• In addition to improvement in ammonia and
nitrite levels, the treated ponds also acquired
balance of heterotrophic bacteria, algae and bluegreen cyano bacteria indicated by the blue-green
pond colour.
• PondProtect has high salinity tolerance and is
active in salinity levels as high as 35 ppt.
Water colour/density of treated pond
Commercial Benefits
• Yield increase
• Weight increase
• FCR decrease
• Profit increase
In trials conducted using PondProtect, treated ponds showed an
average yield improvement of 20%, size improvement of 20%, and
FCR reduction of 20%, resulting in an average gross profit increase
of 55% per pond.
With PondProtect, the farmer can prevent toxic conditions caused
by ammonia and nitrite buildup which lead to stress in the shrimp,
decreased yield and increased mortality rate. PondProtect helps
maintain a healthy pond environment and improves sales and
profitability for the farmer.
Application Details
PondProtect
Shrimp Farms
Dosage
For Prevention
For Emergency Conditions
Initial
3 - 4 Kg/ha
3 - 4 Kg/ha first application
If ammonia or nitrite levels persist, reapply
1 - 2 Kg/ha, 3 days after first application
Maintenance
1 - 2 Kg/ha, per week,
1 week after first application*
*Note: The impact of Ammonia and Nitrite is most serious during
15 - 35 Days of Culture (DOC) and after 90 DOC.
The rates shown above apply under the following conditions:
• DO > 4
• Alkalinity > 100 ppm • Pond depth = 1.5 m
Shrimp hatcheries
For Prevention
For Emergency Conditions
30 gm/1000 liters every 3 days
100 gm/1000 liters
Some size and color variation of product will not affect performance.
Product Specifications
Safety
Compositon
This product contains only Bio safety Level Class I bacteria
which have been fully identified by 16S rDNA sequencing
conducted at MIDI Laboratories, Newark, Delaware, USA.
They are confirmed to be non-pathogenic, non-genetically
manipulated, non-harmful species of environmentally
beneficial microorganisms. These natural bacteria are typically
present in all known regions of the world, including shrimp
pond water and bottom solids.
Blend of two natural non-pathogenic nitrification strains Nitrosomonas eutropha
and Nitrobacter winogradskyi
Activity Ammonia oxidation rate 1000 - 1200 mg N-NH3/Kg/hr
Appearance
Tan, free-flowing powder
Odour
Yeast-like
Optimum pH range
7.5 - 8.3
Shelf Life
One year at 23°C
Optimum temperature range
27°- 32°C
Salinity Range
Up to 36 ppt
Storage and Handling
Product Code: 7003046
Store in cool conditions (23°C or less) up to one year. Can be
stored at 35°C up to 3 months.
Available Packaging
• 5 x 200 gm Solupaks™ in zip-locked foil bags /1 kg box
Novozymes Biologicals, Inc. • 5400 Corporate Circle • Salem, VA 24153
Orders: 800.284.7435 • Fax Orders: 540.389.9173
Sales and Marketing: 800.859.2972 • Fax: 540.389.2688
Novozymes is the biotech-based world leader in enzymes and microorganisms. Using nature’s own technologies, we continuously expand the frontiers of biological solutions to improve industrial performance everywhere. Novozymes’
microorganisms business and all related activities are the responsibility of Novozymes Biologicals, Inc., a wholly owned subsidiary of Novozymes A/S. Laws, regulations and third party rights may prevent customers from importing,
processing, applying and/or reselling certain products in a given manner. It is the responsibility of the customer that their specific use of products of Novozymes does not infringe relevant laws and regulations and, furthermore does not
infringe patents or other third party rights. The contents of this document are subject to change without further notice.
© Novozymes Biologicals, Inc. • www.novozymes.com/microorganisms
Revised 10/25/2007
2007-01087