BSC 2011L – Principles of Biology 2 Lab Lab Topic 19 – Animal Diversity II: Nematoda, Arthropoda, Echinodermata, and Chordata Lab Topic 19: Animal Diversity II • This lab topic is a continuation of observations of • • • • • organisms in the animal kingdom (clade Metazoa) as discussed in lab topic 18. In this lab we will look at examples of nematodes and arthropods (clade Ecdysozoa) and two deuterostome phyla (Echinodermata and Chordata) 1. What clues do similarities and differences among organisms provide about phylogenetic relationships? 2. How is body form related to function? 3. How is body form related to environment and lifestyle? 4. What characteristics can be the criteria for major branching points in producing a phylogenetic tree? Exercise 19.1 – Nematoda • Roundworms (Ascaris) • Materials • Introduction • Procedure • Results – 1. Sketch the cross-section of a female Ascaris – 2. List some features of Ascaris that are possible adaptations to parasitic life. – 3. Complete Table 19.1 for roundworm characteristics • Discussion – 1. Discuss the significance of the an animal’s having two separate openings to the digestive tract. – 2. What are the advantages of a body cavity being present in an animal? 4 5 Exercise 19.2 – Phylum Arthropoda • • • • • Lab Study A. Crayfish (Cambarus) Materials Introduction Procedure Results – 1. Complete Tables 19.1 and 19.2 for crayfish characteristics • Discussion – How does the pattern of segmentation differ in the crayfish and the earthworm studied in lab topic 18? Exercise 19.2 – Phylum Arthropoda • • • • • Lab Study B. Grasshoppers (Romalea) Materials Introduction Procedure Results – 1. Complete Tables 19.1 and 19.2 for grasshopper characteristics • Discussion – How does the pattern of segmentation differ in the crayfish and the earthworm studied in lab topic 18? Anatomy of a Grasshopper Anatomy of a grasshopper, an insect Abdomen Thorax Head Compound eye Antennae Heart Cerebral ganglion Dorsal artery Crop Anus Vagina Malpighian tubules Ovary Tracheal tubes Mouthparts Nerve cords Exercise 19.3 – Phylum Echinodermata • • • • • Sea Star Materials Introduction Procedure Results – 1. Complete Table 19.1 for the sea star characteristics and complete Table 19.2 in the Applying Your Knowledge section • Discussion – Answer the four discussion questions at the end of this exercise Anus Stomach Spine Gills Central disk Digestive glands Madreporite Radial nerve Ring canal Gonads Ampulla Podium Radial canal Tube feet 14 (a) A sea star (class Asteroidea) (b) A brittle star (class Ophiuroidea) (c) A sea urchin (class Echinoidea) (d) A feather star (class Crinoidea) (e) A sea cucumber (class Holothuroidea) (f) A sea daisy (class Concentricycloidea) Exercise 19.4 – Phylum Chordata • • • • • Lab Study A. Lancelets (Branchiostoma) Materials Introduction Procedure Results – 1. Complete the diagram of a lancelet cross section in Figure 19.10. Label all of the structures listed in step 4 of the Procedure section. – 2. Complete Table 19.1 and Table 19.2 in the Applying Your Knowledge section • Discussion – Describe the uniquely chordate features that you have detected in the lancelet that were not present in the animals previously studied. 17 Chordate characteristics Dorsal, hollow nerve cord Muscle segments Notochord Mouth Anus Muscular, post-anal tail Pharyngeal slits or clefts The lancelet, a cephalochordate Cirri 2 cm Mouth Pharyngeal slits Atrium Notochord Digestive tract Atriopore Dorsal, hollow nerve cord Segmental muscles Anus Tail Cross-section of Branchiostoma Exercise 19.4 – Phylum Chordata • • • • • Lab Study B. Fetal pigs (Sus scrofa) Materials Introduction Procedure Results – Complete Table 19.1 for the pig characteristics. – Use this information to complete Table 19.2 and answer questions in the Applying Your Knowledge section that follows. • Questions for Review – Complete questions 1-2 • Applying Your Knowledge – Complete questions 1-8
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