Indian Journal of Natural Products and Resources Vol. 3(4), December 2012, pp. 493-500 Suitability of tin cans and glass jars for processing of peach fruits in juice Manisha Kaushal* and K D Sharma Department of Food Science and Technology, Dr Y S Parmar University of Horticulture and Forestry, Solan-173 230, Himachal Pradesh, India Received 29 August 2011; Accepted 16 July 2012 Suitability of processing of lye peeled peach halves of cultivar ‘July Elberta’ in A 2½ size tin cans and 500 g glass jars in a covering medium of 40oB syrup in different proportions (10-40%) containing either peach pulp or enzymatically extracted (pectinase @ 0.5%) peach juice fortified with or without ascorbic acid (500 ppm) was evaluated on the basis of storability, nutritional value and sensory attributes. Cut out analysis of canned peach halves after 3 and 6 months revealed that both the packages irrespective of the treatments met FPO specifications for vacuum and drained weight. On the basis of physico-chemical and sensory evaluation it was observed that higher retention of ascorbic acid and carotenoids was observed in cans as compared to jars. Further, vitamin C fortified treatments had a higher retention of ascorbic acid both in cans (24.7 mg/100 g) and glass jars (18.54 mg/100 g) than that of unfortified treatment having mean values 10.91 and 9.5 mg/100 g for cans and jars, respectively while, the vitamin C content in control samples (40oB sucrose syrup with 0.3% citric acid) was recorded as 3.91 mg/100 g after 6 months of storage. Addition of peach pulp in covering medium witnessed lesser extent of non-enzymatic browning as compared to enzymatically extracted juice when used in a covering medium. Data pertaining to non-enzymatic browning showed slightly higher values in glass jars (0.092) as compared to the tin cans (0.085). However, no apparent spoilage was noticed in cans and jars throughout the study period of 6 months. It was suggested that conventional processing of peach halves in sucrose syrup in tin cans and glass jars can successfully be replaced with a covering medium containing 30-40% of peach pulp. Keywords: Jars, Juice, Peach, Processing, Prunus persica, Pulp, Tin cans. IPC code; Int. cl. (2011.01) A23B 7/00, B65B 25/04 Introduction Peach (Prunus persica Batsch) is one of the important stone fruits grown in temperate regions of the world. World peach production is estimated to be 18428913 tonnes where India contributes 150,000 tonnes1. Fresh fruits are attractive, delicious and highly nutritious as they contain substantially good amount of carbohydrates, proteins, ascorbic acid, potassium and niacin as compared to majority of other common fruits. Due to perishable nature of the fruit, non-availability of storage facilities in the growing area and lack of cold chain for transportation, the orchard owners get unsatisfactory returns for their produce. The commercial practice of canning of peach halves in sugar syrup results in masking of original fruit flavour and wastage of the sugar syrup. Many authors have suggested replacing sugar syrups with corn syrup, apple concentrate, apples juice and mango pulp for fruit canning2-5. —————— *Correspondent author: E-mail: [email protected] Therefore, to make the fruits available to the consumers throughout the year and to have better income to the growers, preservation of fruit halves in fruit juice could be a good alternative. With the increase in domestic consumption and thrust towards export of processed products, packaging is considered as an important marketing tool. Processing of fruits is mostly done in tin cans resulting into a high price of canned products thus making them unaffordable to a common man. Thus packaging of fruit halves in light weight glass jars could be best alternative as they will be available at reasonable cost to the consumers. Therefore, the study was undertaken to determine the acceptability of processing of peach halves in covering media containing peach juice and pulp in tin cans and glass jars with respect to their quality and shelf-life. Materials and Methods Firm ripe fruits of peach cultivar ‘July Elberta’ obtained from Rajgarh area of Himachal Pradesh were manually sorted, graded and washed thoroughly. The 494 INDIAN J NAT PROD RESOUR, DECEMBER 2012 fruits were lye peeled (1.0% boiling caustic soda for 50 sec), halved, destoned and processed in a covering media of 40°B containing 10-40% peach pulp or enzymatically extracted (pectinase 0.5%) peach juice fortified with or without ascorbic acid (500 ppm) both in A 2½ tin cans and glass jars (500 g). The ripe peach fruit were used for pulp extraction where the fruits were cooked for 10-15 minutes after adding 10% water followed by passing the whole mass through the pulper. For juice extraction, pulp was enzymatically clarified (pectinases @ 0.5% at 40°C for 4 h), heated to 95°C to inactivate the added enzyme and then stored in sterilized glass bottles by pasteurization. The conventionally canned fruit halves in sugar syrup of 40°B with 0.3% citric acid served as control. The processing of tin cans and glass jars was done as per the standard method6 (Plate 1 a,b) and were stored at ambient temperature (14-30°C) for 6 months. The physico-chemical analysis of fresh and canned peach fruits was carried out according to the procedure described elsewere7,8. The sensory evaluation of prepared products was carried out by a panel of 7 semi-trained judges on a 9 point Hedonic scale9 for parameters like colour, flavour, texture, taste and overall acceptability. Triplicate determination was made for each physico-chemical attribute by taking 10 fruits for each replication. Completely Randomized Design (factorial) was used to analyze the data of quantitative estimation of various physico-chemical characteristics10. Results and Discussion Fresh fruits of ‘July Elberta’ were analyzed for their physico-chemical characteristics and the data is summarized in Table 1. The average fruit length and diameter of fruit was determined as 5.82 and 5.95 cm with an average weight of 82.60 g and firmness of 5.23 kg/cm2. Chemically, the fruit contained 11.43% total soluble solids with an acidity of 0.76% as malic acid. The fruits contained 690 µg/100 g and 16.40 mg/100 g of vitamin A and C, respectively. Thus it is apparent from the data that the fruits were suitable for canning which was in conformation with other authors11-14. Cut out analysis for physical attributes The results of cut out analysis of peach halves canned in A 2½ cans and glass jars stored at ambient temperature (14-30°C) revealed that the vacuum decreased during the storage period of 6 months although well within the FPO specifications (Table 2). The maximum vacuum in cans was observed in T14 (40% peach pulp in covering media of 40oB) and T9 (20% peach juice in covering media of 40oB containing 500 ppm of ascorbic acid) having a value Table 1 Physico-chemical characteristics of fresh peach fruits Plate 1 Peach halves: (a) in tin cans, (b) in glass jars S. No. Parameters Mean+SD* 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Length (cm) Diameter (cm) Weight (g) Specific gravity Firmness (kg/cm2) Moisture (%) Total soluble solids (oBrix) Titratable acidity (% malic acid) Ascorbic acid (mg/100 g) 5.82+0.021 5.95+0.03 82.60+4.96 1.09+0.02 5.23+0.30 88.1+0.80 11.43+0.17 0.76+0.02 16.40+0.52 690.0+0.12 Total carotenoids (µg/100 g) * Average of 3 observations (10 fruits per observation) KAUSHAL & SHARMA: SUITABILITY OF TIN CANS AND GLASS JARS FOR PEACH FRUITS PROCESSING 495 Table 2 Effect of treatment and storage period on the vacuum (kg/cm2) of the processed peaches in tin cans and glass jars Storage period 3 months Treatment ** Control (T1) 10% peach pulp (T2) 10% peach juice (T3) 10% peach pulp* (T4) 10% peach juice* (T5) 20% peach pulp (T6) 20% peach juice (T7) 20% peach pulp* (T8) 20% peach juice* (T9) 30% peach pulp (T10) 30% peach juice (T11) 30% peach pulp* (T12) 30% peach juice* (T13) 40% peach pulp (T14) 40% peach juice (T15) 40% peach pulp* (T16) 40% peach juice (T17) Mean Mean (P) 6 months Mean (T) Can (P1) Jar (P2) Mean Can (P1) Jar (P2) Mean 0.60 0.60 0.56 0.55 0.60 0.60 0.58 0.56 0.60 0.58 0.56 0.58 0.56 0.60 0.60 0.60 0.60 0.58 0.56 0.60 0.55 0.58 0.55 0.56 0.60 0.53 0.58 0.56 0.58 0.58 0.55 0.56 0.56 0.60 0.56 0.60 0.57 0.55 0.60 0.58 0.57 0.55 0.58 0.60 0.56 0.57 0.58 0.58 0.57 0.57 0.56 0.58 0.60 0.58 0.60 0.58 0.55 0.50 0.51 0.54 0.50 0.50 0.51 0.53 0.56 0.50 0.53 0.55 0.55 0.56 0.53 0.53 0.55 0.53 0.50 0.51 0.58 0.51 0.56 0.51 0.50 0.53 0.51 0.55 0.50 0.53 0.53 0.56 0.56 0.53 0.50 0.53 0.53 0.51 0.55 0.53 0.53 0.51 0.51 0.53 0.54 0.53 0.52 0.54 0.54 0.56 0.55 0.53 0.53 0.53 0.57 0.55 0.56 0.54 0.55 0.56 0.54 0.55 0.56 0.56 0.55 0.56 0.55 0.57 0.58 0.56 0.57 * Treatments with ascorbic acid @ 500 ppm ** Total soluble solids 40oBrix Effect CD0.05 Effect CD0.05 Treatment NS Treatment × Package 0.04 Package NS Treatment × Storage 0.04 Storage 0.01 Package × Storage NS Treatment × Package × Storage 0.06 of 0.56 kg/cm2 which was statistically at par with other treatments like T17, T13, T12 and T1. In jars, maximum vacuum of 0.58 was observed in T3 (10% peach pulp in covering media of 40oB), followed by 0.56 kg/cm2 in T5, T14 and T15. Data showed that mean vacuum in cans was 0.56 while in jars was 0.55 kg/cm2 which was statistically at par with each other which was in agreement with various authors15-17. Further, both the packages had a non significant effect on the drained weight of the canned peach halves. The mean drained weight in cans and jars after 6 months of storage was 56.09 and 57.05%, respectively. Cut out analysis for chemical attributes Nutritional evaluation of canned peach halves both in A 2½ tin cans and glass jars showed that the retention of ascorbic acid was comparatively higher in cans than the glass jars for the same treatment throughout the storage period (Table 3). Three months storage data revealed that mean ascorbic acid in cans was 12.56 mg/100 g while in jars it was 10.91 mg/ 100 g though maximum value of ascorbic acid was recorded in treatment T17 (20.65 mg/100 g) for both the packages. Six months analysis of stored peach halves in both the packages observed mean value of ascorbic acid as 10.69 in cans as against 9.40 mg/ 100 g in glass jars17,18. Statistically significant differences were observed in ascorbic acid for treatments, storage, and packages and their interactions. The difference of packages with reference to ascorbic acid was found significant as the packaging mean denotes 11.63 mg/100 g value for cans and 10.16 mg/100 g for jars. The storage mean was also significant with respect to ascorbic acid as the respective values for cans and jars were 11.74 and 10.05 mg/100 g. INDIAN J NAT PROD RESOUR, DECEMBER 2012 496 Table 3 Effect of treatment and storage period on ascorbic acid (mg/100 g) of the processed peaches in tin cans and glass jars Storage period Treatment ** Control (T1) 10% peach pulp (T2) 10% peach juice (T3) 10% peach pulp* (T4) 10% peach juice* (T5) 20% peach pulp (T6) 20% peach juice (T7) 20% peach pulp* (T8) 20% peach juice* (T9) 30% peach pulp (T10) 30% peach juice (T11) 30% peach pulp* (T12) 30% peach juice* (T13) 40% peach pulp (T14) 40% peach juice (T15) 40% peach pulp* (T16) 40% peach juice (T17) Mean Mean (P) 3 months 6 months Mean (T) Can (P1) Jar (P2) Mean Can (P1) Jar (P2) Mean 5.03 6.02 6.10 14.20 15.63 6.13 6.50 16.27 18.17 7.32 8.07 18.64 19.30 10.40 10.91 20.17 24.70 12.56 11.63 3.90 5.77 6.07 11.30 12.17 5.92 5.99 15.23 15.75 6.86 7.29 16.26 17.68 9.32 9.50 17.93 18.54 10.91 10.16 4.47 5.90 6.09 12.75 13.90 6.03 6.25 15.75 16.96 7.09 7.68 17.45 18.49 9.86 10.21 19.05 21.62 11.74 4.20 5.03 5.17 11.93 12.34 4.83 5.76 14.48 15.33 6.71 7.47 16.37 17.15 7.61 8.93 16.10 22.48 10.69 3.61 4.17 5.10 9.49 10.67 4.17 4.93 13.18 13.73 6.13 6.83 14.93 15.62 7.17 7.94 15.32 16.87 9.40 3.91 4.60 5.14 10.71 11.51 4.50 5.35 13.83 14.53 6.42 7.15 15.65 16.39 7.39 8.44 15.71 19.67 10.05 * Treatments with ascorbic acid @ 500 ppm ** Total soluble solids 40oBrix Effect CD0.05 Treatment 0.33 Effect Treatment × Package CD0.05 0.47 Package 0.11 Treatment × Storage 0.47 Storage 0.11 Package × Storage 0.16 Treatment × Package × Storage 0.67 Similar to ascorbic acid, the effect of treatment, storage and package was found significant on the carotenoids content of peach halves. Carotenoid content was higher in cans as compared to glass jars due to its permeability to light which is the main causative agent for the destruction of the precursor of vitamin A19. A minimum carotenoid content after 3 months of storage was observed as 429.0 and 425.0 µg/100 g in cans and jars, respectively in treatments T1 (control). Maximum carotenoid was found in treatment T17 for both cans (458.70 µg/100 g) and jars (438.10 µg/100 g) after three months of storage (Table 4). After 6 months of storage similar trend was observed in retention of carotenoids at ambient temperature where the minimum value was found in treatment T1 (control) in cans (428.0 µg/100 g) and jars (411.90 µg/100 g) and the maximum value 4.19 5.25 5.62 11.73 12.71 5.27 5.80 14.79 15.75 6.76 7.42 16.55 17.44 8.63 9.33 17.38 20.65 was observed in treatment T17 (40% peach juice in covering media of 40°B) as 445.90 and 418.50 µg/100 g in cans and jars, respectively. The mean carotenoid content in cans and jars were observed as 442.14 and 423.69 µg/100 g, respectively. The mean values of treatment × storage varied from 420.35 (T1) to 432.20 (T17). The highest mean value of the treatments irrespective of packaging and storage was 440.3 µg/100 g in T17 where treatments T16 and T15 were statistically at par with T17 with a mean T value of 439.85 and 439.28 µg/100 g, respectively. Mitchell et al20 and Kader21 also showed that peaches loose approximately 42-50% of their total carotenoids during exhausting process. Data pertaining to non enzymatic browning (NEB) in Table 5 showed that irrespective of the treatments, comparatively more NEB was observed in glass jars KAUSHAL & SHARMA: SUITABILITY OF TIN CANS AND GLASS JARS FOR PEACH FRUITS PROCESSING 497 Table 4 Effect of treatment and storage period on the carotenoids (µg/100 g) of processed peaches in tin cans and glass jars Storage period Treatment ** Control (T1) 10% peach pulp (T2) 10% peach juice (T3) 10% peach pulp* (T4) 10% peach juice* (T5) 20% peach pulp (T6) 20% peach juice (T7) 20% peach pulp* (T8) 20% peach juice* (T9) 30% peach pulp (T10) 30% peach juice (T11) 30% peach pulp* (T12) 30% peach juice* (T13) 40% peach pulp (T14) 40% peach juice (T15) 40% peach pulp* (T16) 40% peach juice (T17) Mean Mean (P) 3 months 6 months Mean (T) Can (P1) Jar (P2) Mean Can (P1) Jar (P2) Mean 429.00 432.70 437.00 438.00 438.70 445.00 446.00 446.00 449.00 451.30 450.30 453.00 453.70 455.70 456.70 457.00 458.70 446.93 442.14 425.80 428.20 428.60 428.70 428.90 429.50 429.60 430.70 431.60 432.20 435.40 436.30 437.70 437.30 438.90 438.10 432.16 423.16 423.69 427.40 430.45 432.80 433.35 433.80 437.25 437.65 437.80 439.85 441.45 441.25 444.20 445.00 446.70 447.00 447.95 448.40 439.55 428.80 430.70 430.90 431.40 431.90 432.10 432.80 433.30 433.50 437.20 440.70 444.50 444.90 445.10 445.40 445.60 445.90 437.34 411.90 412.30 412.60 412.70 413.50 414.00 414.60 414.90 415.20 416.00 416.20 416.80 416.90 417.10 417.70 417.90 418.50 415.22 420.35 421.50 421.75 422.05 422.70 423.05 423.70 424.10 424.35 426.60 428.45 430.65 430.90 431.10 431.55 431.75 432.20 426.28 * Treatments with ascorbic acid @ 500 ppm ** Total soluble solids 40oBrix Effect CD0.05 Treatment 1.10 Package 0.38 Effect Treatment × Package Treatment × Storage CD0.05 1.56 1.56 Storage Package × Storage 0.54 Treatment × Package × Storage 2.21 0.38 as compared to cans. The maximum value of OD440nm during 3 and 6 months analysis was observed in jars as 0.110 and 0.185, respectively while in cans the values recorded were 0.081 and 0.183, respectively during the same period. A minimum value of NEB was detected in T16 (40% peach pulp in covering media of 40°B containing 500 ppm ascorbic acid) as 0.032 and 0.037 in cans and jars, respectively in 3 months and 0.056 and 0.075 at 6 months storage, respectively. Overall the mean values of non enzymatic knowing in both the packages where 0.085 and 0.092 for cans and jars, respectively. Treatment, package, storage and their interactions had a significant effect on the NEB. The maximum mean value of the treatment × storage interaction was 0.0095 for T1 (Control) with a minimum of 0.035 for T16 (40% peach pulp in a covering media of 40°B containing 423.88 425.98 427.28 427.70 428.30 430.15 430.68 430.90 432.10 434.03 434.85 437.43 438.00 438.90 439.28 439.85 440.30 500 ppm ascorbic acid). It has been reported that substrate, enzyme and oxygen are three main factors responsible for browning of canned products19. Cut out analysis for sensory scores The scores for overall acceptability in canned peaches (Table 6) varied from 4.93 to 7.50 in cans for treatment T1 and T14, respectively during 3 months of storage at ambient temperature. In glass jars, the maximum and a minimum value of 6.14 and 4.21 were also scored by T14 and T1 treatments, respectively. Throughout the storage, the can packed halves had comparatively higher score to that of glass jars though none of the treatments were rejected by the panelists. Significant decrease in overall acceptability was observed for all treatments irrespective of packages during 6 months of storage INDIAN J NAT PROD RESOUR, DECEMBER 2012 498 Table 5 Effect of treatment and storage period on non-enzymatic browning (OD at 440 nm) of processed peaches in tin cans and glass jars Storage period Treatment ** Control (T1) 10% peach pulp (T2) 10% peach juice (T3) 10% peach pulp* (T4) 10% peach juice* (T5) 20% peach pulp (T6) 20% peach juice (T7) 20% peach pulp* (T8) 20% peach juice* (T9) 30% peach pulp (T10) 30% peach juice (T11) 30% peach pulp* (T12) 30% peach juice* (T13) 40% peach pulp (T14) 40% peach juice (T15) 40% peach pulp* (T16) 40% peach juice (T17) Mean Mean (P) 3 months 6 months Mean (T) Can (P1) Jar (P2) Mean Can (P1) Jar (P2) Mean 0.081 0.072 0.061 0.051 0.055 0.059 0.079 0.041 0.039 0.056 0.071 0.036 0.042 0.061 0.074 0.032 0.046 0.056 0.085 0.110 0.076 0.066 0.054 0.056 0.061 0.081 0.043 0.042 0.059 0.075 0.041 0.047 0.064 0.075 0.037 0.052 0.061 0.092 0.095 0.074 0.064 0.053 0.056 0.060 0.080 0.042 0.041 0.058 0.074 0.039 0.045 0.063 0.075 0.035 0.049 0.059 0.183 0.147 0.171 0.078 0.085 0.128 0.180 0.047 0.064 0.122 0.178 0.064 0.073 0.106 0.161 0.056 0.079 0.113 0.185 0.152 0.181 0.084 0.092 0.136 0.184 0.053 0.064 0.152 0.182 0.067 0.082 0.107 0.171 0.075 0.106 0.122 0.184 0.150 0.176 0.081 0.089 0.132 0.182 0.050 0.064 0.137 0.180 0.066 0.076 0.106 0.166 0.066 0.093 0.118 * Treatments with ascorbic acid @ 500 ppm ** Total soluble solids 40oBrix Effect CD0.05 Treatment 0.0020 Package 0.0008 Storage 0.0008 Effect Treatment × Package Treatment × Storage Package × Storage Treatment × Package × Storage 0.140 0.125 0.107 0.067 0.073 0.096 0.130 0.046 0.053 0.120 0.127 0.052 0.061 0.084 0.091 0.051 0.071 CD0.05 0.0033 0.0033 0.0011 0.0047 Table 6 Effect of treatment and storage period on overall acceptability (sensory characteristic) of processed peaches in tin cans and glass jars Storage period Treatment ** Control (T1) 10% peach pulp (T2) 10% peach juice (T3) 10% peach pulp* (T4) 10% peach juice* (T5) 20% peach pulp (T6) 20% peach juice (T7) 20% peach pulp* (T8) 20% peach juice* (T9) 30% peach pulp (T10) 30% peach juice (T11) 3 months 6 months Mean (T) Can (P1) Jar (P2) Mean Can (P1) Jar (P2) Mean 4.93 6.43 5.67 6.21 6.36 6.50 6.27 6.43 6.29 6.71 6.64 4.21 5.14 5.00 5.21 5.93 5.00 4.93 5.00 5.71 5.36 4.57 4.57 5.79 5.29 5.71 6.14 5.75 5.61 5.71 6.00 6.04 5.11 3.86 5.64 5.36 6.29 5.79 6.21 6.07 6.07 5.14 6.57 5.36 3.57 4.71 4.14 5.29 5.00 5.43 4.43 4.86 4.86 5.00 4.43 3.71 5.18 4.75 5.79 5.39 5.82 5.25 5.46 5.00 5.79 4.89 4.14 5.49 5.02 5.75 5.79 5.79 5.55 5.58 5.50 5.79 5.00 (Contd.) KAUSHAL & SHARMA: SUITABILITY OF TIN CANS AND GLASS JARS FOR PEACH FRUITS PROCESSING 499 Table 6 Effect of treatment and storage period on overall acceptability (sensory characteristic) of processed peaches in tin cans and glass jars (Contd.) Storage period 3 months Treatment ** 30% peach pulp* (T12) 30% peach juice* (T13) 40% peach pulp (T14) 40% peach juice (T15) 40% peach pulp* (T16) 40% peach juice (T17) Mean Mean (P) 6 months Mean (T) Can (P1) Jar (P2) Mean Can (P1) Jar (P2) Mean 6.21 6.14 7.50 6.14 5.79 6.36 6.26 5.99 5.03 5.00 6.14 5.71 5.29 5.00 5.24 5.01 5.57 5.57 6.82 5.93 5.54 5.68 5.73 5.21 5.73 6.79 5.57 5.93 6.00 5.72 4.71 4.86 5.71 5.43 4.50 4.21 4.77 4.96 5.14 6.25 5.50 5.21 5.11 5.25 * Treatments with ascorbic acid @ 500 ppm ** Total soluble solids 40oBrix Effect CD0.05 Treatment 0.35 Package 0.12 Storage 0.12 Effect Treatment × Package Treatment × Storage Package × Storage Treatment × Package × Storage 5.27 5.36 6.54 5.72 5.38 5.39 CD0.05 0.49 0.49 NS NS at ambient temperature. Maximum score of 6.79 was again obtained by treatment T14 followed by T10, T4 and T6 having a sensory of 6.57, 6.29 and 6.21, respectively. Highest and lowest sensory score for the overall acceptability in jars during 6 months of storage was also assigned to the treatments T14 and T1 as 5.71 and 3.57, respectively. The scores of rest of the treatments ranged between 3.57 and 3.71. It has been earlier reported that addition of acids leads to a firmer and crispy texture of canned peaches19. Further it has been reported that the canned peach halves after 50 days of storage were found to be the best and the products remained in acceptable range upto 200 days21,22. The mean treatment × storage interaction for 6 months storage varied from 3.71 (T1) to 6.25 (T14). The effect of treatments was found significant on the sensory scores of overall acceptability ranging from 4.14 (T1) followed by 5.02 for T3 to 6.54 for T14, respectively. The interaction of package × storage × and treatment × package × storage were non significant. 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