RNA
FirstChoice® Tumor RNA
Jump Start Your Expression Analyses
with FirstChoice® Tumor RNA
• Consistent high quality RNA from non-pooled, single tumors
• Pathology and quality documentation included
• Normal adjacent tissue also offered
• Custom isolation service option also available
Lymphoma Tumor RNA
Overnight
The Perfect Start to Gene Expression Profiling
M
-20º C
37º C
Our FirstChoice® RNAs save time and money while providing access to RNA from tumor
tissues that might otherwise be difficult to obtain. Furthermore, our isolation experts have
used their experience and cost effective resources to isolate tumor RNA suitable for even the
most sensitive analyses. We provide high quality total RNA purified from individual human
tumors and the corresponding normal adjacent tissue.
Whether you want to examine gene expression in individual tumors or screen multiple
2.5
quality RNA is perfect for gene expression profiling such as:
2.0
• Identifying differentially expressed RNAs via array analysis
• Detecting and quantifying individual targets in tumor vs. normal
human RNA by RT-PCR
• Characterizing splicing variations in tumors
Fluorescence
tumors from a given tissue, Ambion has done the hard work for you. Ambion’s high
FirstChoice® Lymphoma
Tumor RNA –20º C
FirstChoice® Lymphoma
Tumor RNA 37º C
1.5
1.0
0.5
0.0
18S
19
24
29
34
39
28S
44
49
54
59
64
69
Time (seconds)
Quality You Can Trust
All of Ambion’s FirstChoice Tumor RNA samples are subjected to our stringent Quality
Control assays to ensure that the RNA is intact, stable, and free of contaminants; such
as genomic DNA (<0.05%).
Figure 1. Human Lymphoma Total RNA Stability and Quality.
FirstChoice lymphoma tumor RNA was stored overnight at either -20ºC
or 37ºC. The RNA was then evaluated on a denaturing agarose gel by
electrophoresis and on an Agilent 2100 bioanalyzer. Distinct bands on
the gel and a flat baseline on the bioanalyzer for both overnight temperatures indicates the RNA is intact and free from contaminating nucleases.
The approximate 2:1 ratio of rRNA (28S and 18S) bands and peaks
further demonstrates the integrity and stability of FirstChoice RNA, even
after overnight storage at 37ºC.
RNAi • miRNA • RNA SAMPLE PREPARATION • RNA AMPLIFICATION • RNA RESEARCH PRODUCTS
Ready for Shipment
FirstChoice® tumor RNA products include 5 µg of total RNA iso-
Ethical Tissue Procurement
lated from individual human tumors and the option for 5 µg of
Ambion is committed to maintaining the highest level of
normal adjacent tissue (NAT).
ethical standards in regards to all tissue that is used in the
Each product is derived from a
preparation of RNA and RNA-derived products. We have
single donor and has not been pooled with other RNA.
developed an extensive network of ethical tissue procurement
Custom Isolation
partners who are dedicated to taking all available measures
If you need high quality Tumor RNA not found in our existing
library, Ambion’s custom RNA Isolation Services Group will work
directly with you to fulfill your specific research needs.
The
Services Group responds to all inquiries, large or small, and provides custom RNA isolation services such as:
to make certain that each donor has given their fully informed
consent. Ambion has the ability to monitor and audit any of
our procurement partners to ensure all proper consent forms
are on record. We are also committed to protecting the
anonymity of the tissue donors.
• Isolating RNA from tissue that you provide, or
• Utilizing a network of Tissue Procurement Partners to find
specific normal or diseased tissue samples
• Working with you to determine the best and most economical
procedures to provide RNA that is ideally suited for your
downstream application
ORDERING INFORMATION
Tumor only Cat #
Size
Tumor/NAT Cat #
Size
Human Breast RNA
7221
5 µg
7220
2 x 5 µg
Contact [email protected]
Human Lung RNA
7225
5 µg
7224
2 x 5 µg
with questions or requests for more information.
Human Pancreas RNA
7229
5 µg
N/A
N/A
Human Uterus RNA
7233
5 µg
7232
2 x 5 µg
Human Colon RNA
7237
5 µg
7236
2 x 5 µg
Human Thyroid RNA
7241
5 µg
7240
2 x 5 µg
Human Liver RNA
7245
5 µg
7244
2 x 5 µg
Human Bladder RNA
7249
5 µg
7248
2 x 5 µg
Human Kidney RNA
7253
5 µg
7252
2 x 5 µg
Human Ovary RNA
7257
5 µg
7256*
2 x 5 µg
Human Testes RNA
7261
5 µg
7260
2 x 5 µg
Human Stomach RNA
7265
5 µg
7264
2 x 5 µg
Learn more at
www.ambion.com/rna
Product Name
Human Lymphoma RNA
7269
5 µg
7268*
2 x 5 mg
Human Cervix RNA
7277
5 µg
7276
2 x 5 µg
Human Prostate RNA
7289
5 µg
7288
2 x 5 µg
Human Lymph Node RNA
7291
5 µg
N/A
N/A
Human Small Intestine RNA
N/A
N/A
7280
2 x 5 µg
Human Esophagus RNA
N/A
N/A
7284
2 x 5 µg
RNAi • miRNA • RNA SAMPLE PREPARATION • RNA AMPLIFICATION*Tumor/Normal
• RNA RESEARCH
PRODUCTS
Non-adjacent
Tissue
Search our Tumor RNA database
for additional information and product availability
www.ambion.com/catalog/search_TumorRNA.html
Ambion, Inc.
U.S. 800-888-8804 • Canada 800-445-1161
fax +1-512-651-0201
Ambion (Europe) Ltd
tel +44 (0)1480-373-020
fax +44 (0)1480-373-010
Ambion K.K. (Japan)
tel +81 (0)3-5638-2181
fax +81 (0)3-5638-2182
For a list of Ambion direct free
phone numbers and distributors
in your country, go to
www.ambion.com/contact