LA
30%
OFF
R
S Kit
TCR-engineered reference samples
for assay performance control
FE
T
ER
U NC H OF
T-Cell Assay Standard
Universal T-Cell Assay Standard
TERS™ Kit
Produce TCR-engineered reference samples (TERS™)
Calibrate multimer, ICS and ELISPOT assays
Validate antigen-specific T-cell assays
Monitor assay performance
Contact us for further information: [email protected]
JPT Peptide Technologies
www.jpt.com
[email protected]
Contact
T +49-30-6392-7878
F +49-30-6392-7888
USA/Canada
T 1-888-578-2660
F 1-888-578-2666
Innovative Peptide Solutions
Universal T-Cell Assay Standard
TERS™ Kit
Performance Control of T-Cell Assays
The Universal T-Cell Assay Standard TERS™ Kit permits the generation of
human MHC-class-I restricted T-cell receptor (TCR)-engineered reference
samples (TERS™) containing defined numbers of functional, antigen-specific
T-cells by means of an antigen encoding TCR RNA.
Applications
Benefits
• Standardization of MHC Multimer, ICS and
• Comparability of antigen-specific test results
ELISPOT assays
• Validation of antigen-specific tests
• Monitoring of T-cell assay performance over
time, between different platforms or labs
• Calibration of antigen-specific assays
across different time points, platforms, or
laboratories
• Signals are comparable to patient material
• Quantitative information on assay performance
• Easy production of TERS™
Meeting the Lack of Standard Controls
The lack of standard control reagents for immunological T-cell assays has been limiting the comparability
of antigen-specific test results, whether these originate from the same laboratory at different times, from
different platforms, or from different laboratories. The Universal T-Cell Assay Standard TERS™ Kit addresses this need for standardization controls.
How to Produce TERS™
• Transfect PBMCs with provided TCR mRNA
• Optimize electroporation with provided
•
•
•
•
GFP mRNA
Quality control by flow-cytometry
Spike a defined number of TCR-positive T-cells
in untouched PBMCs
Aliquot the suspension and freeze
Use our detailed protocol for all experimental
steps
Selected References
> “Generation of TCR-Engineered T Cells and Their
Use To Control the Performance of T-Cell Assays“
Bidmon et al., J. Immunol. (2015)
TERS was developed by TRON with the help of CIP
and Wallace Coulter Foundation
Flow Cytometry
Transfection with
TCR RNA (α & β)
Production of TCR-engineered reference samples
with our Universal T-Cell
Assay Standard TERS™ Kit)
ELISPOT
PBMCs from
Buffy Coat donor
JPT Peptide Technologies
www.jpt.com
[email protected]
Contact
T +49-30-6392-7878
F +49-30-6392-7888
Cultivation and storage of
transfected and native cells
Pooling as required
Performance
control of assays
USA/Canada
T 1-888-578-2660
F 1-888-578-2666
Innovative Peptide Solutions