BD Transduction Laboratories™
Technical Data Sheet
Purified Mouse Anti-AKAP95
Product Information
610994
50 µg
250 µg/ml
47/AKAP95
Human AKAP95 aa. 460-781
Mouse IgG1, κ
QC Testing: Human
95 kDa
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium
azide.
Material Number:
Size:
Concentration:
Clone:
Immunogen:
Isotype:
Reactivity:
Target MW:
Storage Buffer:
Description
Induction of cAMP results in the activation of the cAMP-dependent protein kinase (PKA) which, in turn, phosphorylates and regulates
downstream effectors. The localization and, hence, the specificity of PKA action is mediated by the interaction of four different PKA
regulatory subunits (RIα, RIβ, RIIα, and RIIβ) and specific PKA anchoring proteins. Several proteins have been identified as PKA type I/II
anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). Several AKAPs (AKAP79/75, AKAP350, and AKAP250)
associate with PKA type II and direct its localization to cortical actin, centrosomes, and filopodia. AKAP95 is a specific nuclear anchoring
protein that contains two zinc finger motifs and an RII binding domain. Although AKAP95 does not interact with RIIβ, it has a high affinity
for RIIα. During interphase, AKAP95 is bound to DNA. However, entry into mitosis results in AKAP95 detachment from the DNA and
association with RIIα. Although the specific function of this AKAP95/RIIα heterodimer is unknown, its formation is cell cycle-dependent and
may be essential for proper progression through the cell cycle.
Western blot analysis of AKAP95 on Jurkat lysate.
Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of
AKAP95.
immunofluorescence staining on FHs 738 (ATCC
HTB-157) cells.
Preparation and Storage
Store undiluted at -20°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Application Notes
Application
Western blot
Routinely Tested
Immunofluorescence
Tested During Development
610994 Rev. 2
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Suggested Companion Products
Catalog Number
554002
611451
Name
HRP Goat Anti-Mouse Ig
Jurkat Cell Lysate
Size
1.0 ml
500 µg
Clone
(none)
(none)
Product Notices
1.
2.
3.
4.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
References
Collas P, Le Guellec K, Taskén K. The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis. J Cell
Biol. 1999; 147(6):1167-1180. (Clone-specific: Functional assay)
Eide T, Coghlan V, Orstavik S. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein,
AKAP95. Exp Cell Res. 1998; 238(2):305-316. (Biology)
Schillace RV, Andrews SF, Liberty GA, Davey MP, Carr DW. Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring
protein in T lymphocytes. J Immunol. 2002; 168(4):1590-1599. (Clone-specific: Immunofluorescence, Western blot)
Steen RL, Cubizolles F, Le Guellec K, Collas P. A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for
chromosome condensation in mitotic extract. J Cell Biol. 2000; 149(3):531-536. (Clone-specific: Western blot)
Steen RL, Martins SB, Taskén K, Collas P. Recruitment of protein phosphatase 1 to the nuclear envelope by A-kinase anchoring protein AKAP149 is a
prerequisite for nuclear lamina assembly. J Cell Biol. 2000; 150(6):1251-1262. (Clone-specific: Western blot)
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