Package Insert ViewRNA™ eZ-L Detection Kit–1-Plex (Red) Part Number: 19500 Product Information Contents: Components listed in Table 1 below. Handling Conditions: Do not freeze reagents. Temperature Limitations: Store at 2–8°C. Avoid temperature fluctuations. Lot Number: Refer to rack label. Use By Date: Store at 2–8°C. Avoid temperature fluctuations Manufacturer: Thermo Fisher Scientific, Inc. Product Description The ViewRNA™ eZ-L Detection Kit–1-Plex (Red) contains the universal reagents that are required to perform automated RNA in situ hybridization (ISH) for formalin-fixed, paraffin-embedded (FFPE) tissue sections on slides. The kit is compatible with Leica Biosystems’ BOND-III Advanced Staining System. The User Guide for the ViewRNA™ eZ-L Detection Kit–1-Plex (Red) can be downloaded from our web site. Safety Information A Safety Data Sheet(s) (SDS) is available on our web site. Please refer to the SDS for each component for hazard information. General Precautions ViewRNA eZ-L Detection Kit–1-Plex (Red) is for laboratory use only. Some components required for this procedure may pose significant health risks. Follow prudent laboratory practices when handling and disposing of carcinogens and toxins. Refer to the manufacturer’s Safety Data Sheet for additional information. Wear appropriate personal protective equipment when using these reagents. At a minimum, wear safety glasses and chemical resistant gloves. Perform all procedural steps in a well-ventilated area at room temperature unless otherwise noted. Discard all reagents in accordance with local, state, and federal laws. CAUTION: Formaldehyde is a poison and an irritant. Avoid contact with skin and mucous membranes. Use in a fume hood. ViewRNA eZ-L Probe Diluent and Amp R1, Amp R2, and Amp R3 contain formamide, a teratogen, irritant, and possible carcinogen. Avoid contact with mucous membranes. Using the ViewRNA™ eZ-L Detection Kit–1-Plex (Red) The user guide for the ViewRNA™ eZ-L Detection Kit–1-Plex (Red) can be downloaded from the product page: www.ThermoFisher.com Operating Leica Biosystems’ BOND-III Advanced Staining System Refer to the Leica BOND User Manual for information about chemical and mechanical hazards, electrical hazards, and additional cautionary information. For Research Use Only. Not for use in diagnostic procedures. Hazard Statements Symbol Synonym GHS08 health hazard Amide C1, Formic amide Hazard Statements H351: Suspected of causing cancer. H360: May damage fertility or the unborn child. H373: May cause damage to organs (blood) through prolonged or repeated exposure if swallowed. Symbol Table Table 1. ViewRNA™ eZ-L Detection Kit–1-Plex (Red), P/N 19500 Quantity Description ViewRNA eZ-L Fix 1 x 4.5 mL Tissue fixative ViewRNA eZ-L Rinse 1 x 27 mL Salt and sodium citrate buffer ViewRNA eZ-L Amp R1 1 x 9 mL Oligonucleotides in hybridization buffer ViewRNA eZ-L Amp R2 1 x 9 mL Oligonucleotides in hybridization buffer ViewRNA eZ-L Amp R3 1 x 9 mL Oligonucleotides in hybridization buffer ViewRNA eZ-L Red1 Red chromogen in aqueous solution ViewRNA eZ-L Red 2 1 x 8.25 mL 1 x 2.7 mL ViewRNA eZ-L Hematoxylin 1 x 9 mL Nuclear counterstain dye Open Container (for ViewRNA eZ-L Amp R4) Part 2 of 2 1 each Empty open container ViewRNA eZ-L Amp R4, 500X 1 x 50 µL Alkaline phosphatase-conjugated oligonucleotides in aqueous solution ViewRNA eZ-L Amp R4 Diluent 1 x 25 mL Hybridization buffer ViewRNA eZ-L Probe Diluent 1 x 40 mL Hybridization buffer Component Cautions Part 1 of 2 Harmful if inhaled. May cause cancer. Causes serious eye irritation. May damage the unborn child. Causes serious eye irritation. May damage the unborn child. Causes serious eye irritation. May damage the unborn child. Alkaline phosphatase substrate in aqueous solution Causes serious eye irritation. May damage the unborn child. 2 ViewRNA™ eZ-L Detection Kit–1-Plex (Red) Table 2. Graphic symbols used for Thermo Fisher Scientific product labels, Package Inserts and User Guides. Symbol / Letters Statement Lot Number Part/Catalog Number Use By YYYY–MM-DD Temperature Limitation Store at 2–8°C. Manufacturer ViewRNA™ eZ-L Detection Kit–1-Plex (Red) 3 Documentation and support Customer and technical support Visit thermofisher.com/support for the latest in services and support, including: Worldwide contact telephone numbers Product support, including: - Product FAQs - Software, patches, and updates Order and web support Limited product warranty Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale found on Life Technologies' website at www.thermofisher.com/us/en/home/global/termsand-conditions.html. If you have any questions, please contact Life Technologies at thermofisher.com/support. Product documentation, including: - User guides, manuals, and protocols - Certificates of Analysis - Safety Data Sheets (SDSs; also known as MSDSs) Note: For SDSs for reagents and chemicals from other manufacturers, contact the manufacturer. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT. Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. Corporate entity: Life Technologies | Carlsbad, CA 92008 USA | Toll Free in USA 1.800.955.6288 ©2017 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. All other trademarks are properties of their respective owners. P/N 703296 For support visit thermofisher.com/support or email [email protected] thermofisher.com 23 January 2017
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