ViewRNA™ eZ-L Detection Kit–1-Plex (Red)

Package Insert
ViewRNA™ eZ-L Detection Kit–1-Plex (Red)
Part Number: 19500
Product Information
Contents: Components listed in Table 1 below.
Handling Conditions: Do not freeze reagents.
Temperature Limitations: Store at 2–8°C. Avoid temperature fluctuations.
Lot Number: Refer to rack label.
Use By Date: Store at 2–8°C. Avoid temperature fluctuations
Manufacturer: Thermo Fisher Scientific, Inc.
Product Description
The ViewRNA™ eZ-L Detection Kit–1-Plex (Red) contains the universal reagents that are required to perform automated RNA in situ
hybridization (ISH) for formalin-fixed, paraffin-embedded (FFPE) tissue sections on slides. The kit is compatible with Leica Biosystems’
BOND-III Advanced Staining System.
The User Guide for the ViewRNA™ eZ-L Detection Kit–1-Plex (Red) can be downloaded from our web site.
Safety Information
A Safety Data Sheet(s) (SDS) is available on our web site. Please refer to the SDS for each component for hazard information.
General Precautions
ViewRNA eZ-L Detection Kit–1-Plex (Red) is for laboratory use only. Some components required for this procedure may pose significant
health risks. Follow prudent laboratory practices when handling and disposing of carcinogens and toxins. Refer to the manufacturer’s
Safety Data Sheet for additional information. Wear appropriate personal protective equipment when using these reagents. At a minimum,
wear safety glasses and chemical resistant gloves. Perform all procedural steps in a well-ventilated area at room temperature unless
otherwise noted. Discard all reagents in accordance with local, state, and federal laws.
CAUTION:

Formaldehyde is a poison and an irritant. Avoid contact with skin and mucous membranes. Use in a fume hood.

ViewRNA eZ-L Probe Diluent and Amp R1, Amp R2, and Amp R3 contain formamide, a teratogen, irritant, and possible carcinogen.
Avoid contact with mucous membranes.
Using the ViewRNA™ eZ-L Detection Kit–1-Plex (Red)
The user guide for the ViewRNA™ eZ-L Detection Kit–1-Plex (Red) can be downloaded from the product page: www.ThermoFisher.com
Operating Leica Biosystems’ BOND-III Advanced Staining System
Refer to the Leica BOND User Manual for information about chemical and mechanical hazards, electrical hazards, and additional
cautionary information.
For Research Use Only. Not for use in diagnostic procedures.
Hazard Statements
Symbol
Synonym
GHS08 health hazard
Amide C1, Formic amide
Hazard Statements

H351: Suspected of causing cancer.

H360: May damage fertility or the unborn child.

H373: May cause damage to organs (blood) through prolonged or repeated exposure if swallowed.
Symbol Table
Table 1. ViewRNA™ eZ-L Detection Kit–1-Plex (Red), P/N 19500
Quantity
Description
ViewRNA eZ-L Fix
1 x 4.5 mL
Tissue fixative
ViewRNA eZ-L Rinse
1 x 27 mL
Salt and sodium citrate buffer
ViewRNA eZ-L Amp R1
1 x 9 mL
Oligonucleotides in hybridization buffer
ViewRNA eZ-L Amp R2
1 x 9 mL
Oligonucleotides in hybridization buffer
ViewRNA eZ-L Amp R3
1 x 9 mL
Oligonucleotides in hybridization buffer
ViewRNA eZ-L Red1
Red chromogen in aqueous solution
ViewRNA eZ-L Red 2
1 x 8.25
mL
1 x 2.7 mL
ViewRNA eZ-L Hematoxylin
1 x 9 mL
Nuclear counterstain dye
Open Container (for ViewRNA eZ-L
Amp R4)
Part 2 of 2
1 each
Empty open container
ViewRNA eZ-L Amp R4, 500X
1 x 50 µL
Alkaline phosphatase-conjugated oligonucleotides in
aqueous solution
ViewRNA eZ-L Amp R4 Diluent
1 x 25 mL
Hybridization buffer
ViewRNA eZ-L Probe Diluent
1 x 40 mL
Hybridization buffer
Component
Cautions
Part 1 of 2
Harmful if inhaled. May
cause cancer.
Causes serious eye irritation.
May damage the unborn child.
Causes serious eye irritation.
May damage the unborn child.
Causes serious eye irritation.
May damage the unborn child.
Alkaline phosphatase substrate in aqueous solution
Causes serious eye irritation.
May damage the unborn child.
2
ViewRNA™ eZ-L Detection Kit–1-Plex (Red)
Table 2. Graphic symbols used for Thermo Fisher Scientific product labels, Package Inserts and User Guides.
Symbol / Letters
Statement
Lot Number
Part/Catalog Number
Use By YYYY–MM-DD
Temperature Limitation Store at 2–8°C.
Manufacturer
ViewRNA™ eZ-L Detection Kit–1-Plex (Red)
3
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
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
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Order and web support


Limited product warranty
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Product documentation, including:
- User guides, manuals, and protocols
- Certificates of Analysis
- Safety Data Sheets (SDSs; also known as
MSDSs)
Note: For SDSs for reagents and chemicals from other
manufacturers, contact the manufacturer.
The information in this guide is subject to change without notice.
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PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
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23 January 2017