Version: 4
Revision Date: 07/20/2016
Purified anti-RNA Polymerase II Antibody (Previously Covance
catalog# MMS-128P)
Catalog# / Size
904001 / 100 µl
Clone
CTD4H8 (See other available formats)
Other Names
DNA-directed RNA polymerase II subunit RPB1, RNA
polymerase II subunit B1, DNA-directed RNA polymerase
II subunit A, RNA-directed RNA polymerase II subunit
RPB1, DNA-directed RNA polymerase III largest subunit
Previously
Covance Catalog# MMS-128P
Isotype
Mouse IgG1
Ave. Rating
★★★★★ 0 reviews
Description
RPB1 is the catalytic and largest component of RNA polymerase II, which
synthesizes mRNA precursors and many functional non-coding RNAs. It forms
the polymerase active center together with RPB2, the second largest
subunit. Polymerase II (Pol II) is the central component of the basal RNA
Western blot probed with anti-RNA
polymerase II transcription machinery. It is composed of mobile elements
Polymerase II antibody (clone CTD4H8) Lane
that move relatively to each other. RPB1 is part of the core element with the
1 : Molecular weight ladder; Lane 2: BSA
central large cleft, the clamp element that moves to open and close the
negative control; Lane 3 : HeLa cell lysate.
cleft, and the jaws that are thought to grab the incoming DNA template. At
the start of transcription, a single DNA template strand of the promoter is
positioned within the central active site cleft of Pol II. Then, a bridging helix
emanates from RPB1 and crosses the cleft near the catalytic site, which acts
as a ratchet that moves the RNA-DNA hybrid through the active site by
switching from straight to bent conformations during each neuocleotide
addition. This promotes translocation of Pol II. Pol II moves on the template
during transcription elongation. Elongation is influenced by the
phosphorylation status of the C-terminal domain (CTD) of Pol II's largest
subunit (RPB1), which serves as a platform for assembling factors that
regulate transcription initiation, elongation, termination, and mRNA
processing. It can act as a RNA-dependent RNA polymerase when
associated with small delta antigen of Hepatitis delta virus, being able to
conform as both a replicate and transcriptase for the viral RNA circular
genome.
Reactivity
Human, Mouse, Rat, Drosophilia, Yeast (S. cerevisiae), Zebrafish
Immunogen
The immunogen used was a peptide containing 10 repeats of the synthetic peptide YSPTSPS
using chemically synthesized phospho-ser5.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the
storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You
can also check your vial or your CoA to find the most accurate storage condition for this
antibody.
Application
WB, IP, ELISA, IF, ChIP
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:500
• IF: 1:500
Application Notes
This antibody is effective in immunoblotting, immunoprecipitation and ELISA. It can also be
used in chromatin immunoprecipitation assays.
The antibody CTD4H8 recognizes the C-terminal repeat of the largest subunit of RNA
polymerase II from HeLa and S. cerevisiae cells. This antibody recognizes both the
phosphorylated and unphosphorylated forms of the RNA polymerase II.
Based on sequence identify, this clone is liable to react with a broad range of species.
Application References
(PubMed link indicates
BioLegend citation)
1. Kristjuhan A, et al. 2002. Mol. Cell. 10:925.
2. Charos AE, et al. 2012. Genome Res. 22:1668. (ChIP)
3. Rafalska-Metcalf IU, et al. 2010. PLoS One 5:e10272. (IF) PubMed
Publication Library
RRID
AB_2565036 (BioLegend Cat. No. 904001)
Antigen Details
Gene ID
5430
UniProt
View information about RNA Polymerase II on UniProt.org
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related Protocols
Western Blotting Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Antibodies and More > Cell Biology > Transcriptional Regulators > RNA Polymerase II >
Antibodies and More > Cell Biology > Signaling/Signaling Intermediates > RNA Polymerase II >
Antibodies and More > Cell Biology > Antigens A-Z > RNA Polymerase II >
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