PowerLecture: Chapter 10 Meiosis and Sexual Reproduction Sexual Reproduction Chromosomes are duplicated in germ cells Germ cells undergo meiosis and cytoplasmic division Cellular descendents of germ cells become gametes Gametes from each parent meet at fertilization Leads to diversity Asexual Reproduction Single parent produces offspring All offspring are genetically identical to one another and to parent Sexual Reproduction Involves Meiosis Gamete production Fertilization Produces genetic variation among offspring Homologous Chromosomes Carry Different Alleles Cell has two of each chromosome One chromosome in each pair from mother, other from father Paternal and maternal chromosomes carry different alleles Sexual Reproduction Shuffles Alleles Through sexual reproduction, offspring inherit new combinations of alleles, which leads to variations in traits This variation in traits is the basis for evolutionary change FLOWERING PLANT anther (where cells that give rise to male gametes originate) ovules, inside an ovary (where cells that give rise to female gametes originate) Fig. 10-3a, p.156 Chromosome Number Sum total of chromosomes in a cell Germ cells are diploid (2n) Gametes are haploid (n) Meiosis halves chromosome number Human Karyotype 1 2 3 6 7 8 13 14 15 19 20 21 4 9 22 5 10 11 16 17 12 18 XX (or XY) Fig. 10-4, p.157 Meiosis: Two Divisions Two consecutive nuclear divisions Meiosis I Meiosis II DNA is not duplicated between divisions Four haploid nuclei form Meiosis I Each homologue in the cell pairs with its partner, then the partners separate p. 158 Meiosis II The two sister chromatids of each duplicated chromosome are separated from each other two chromosomes (unduplicated) one chromosome (duplicated) p. 158 Meiosis I - Stages Prophase I Metaphase I Anaphase I Telophase I Prophase I Each duplicated chromosome pairs with homologue Homologues swap segments (crossing over) Each chromosome becomes attached to spindle Fig. 10-5, p. 158 Metaphase I Homologous Chromosomes are pushed and pulled into the middle of cell Fig. 10-5, p. 158 Anaphase I Homologous chromosomes segregate The sister chromatids remain attached Fig. 10-5, p. 158 Telophase I The chromosomes arrive at opposite poles Usually followed by cytoplasmic division Fig. 10-5, p. 158 Prophase II Microtubules attach to the kinetochores of the duplicated chromosomes Fig. 10-5, p. 158 Metaphase II Duplicated chromosomes line up at the spindle equator, midway between the poles Fig. 10-5, p. 158 Anaphase II Sister chromatids separate to become independent chromosomes Fig. 10-5, p. 158 Telophase II The chromosomes arrive at opposite ends of the cell A nuclear envelope forms around each set of chromosomes Four haploid cells Fig. 10-5, p. 158 MEIOSIS I plasma membrane newly forming microtubules in the cytoplasm PROPHASE I spindle equator (midway between the two poles) METAPHASE I one pair of homologous chromosomes ANAPHASE I TELOPHASE I Fig. 10-5, p.158 there is no DNA replication between the two divisions PROPHASE II MEIOSIS II METAPHASE II ANAPHASE II TELOPHASE II Fig. 10-5b, p.159 Effect of Crossing Over After crossing over, each chromosome contains both maternal and paternal segments Creates new allele combinations in offspring Plant Life Cycle sporophyte zygote fertilization diploid meiosis haploid spores gametes gametophytes Fig. 10-8a, p.162 Animal Life Cycle multicelled body zygote fertilization diploid haploid meiosis gametes Fig. 10-8b, p.162 Fertilization Male and female gametes unite and nuclei fuse Fusion of two haploid nuclei produces diploid nucleus in the zygote Which two gametes unite is random Adds to variation among offspring Factors Contributing to Variation among Offspring Crossing over during prophase I Random alignment of chromosomes at metaphase I Random combination of gametes at fertilization Mitosis & Meiosis Compared Mitosis Functions Meiosis Asexual reproduction Growth, repair Occurs in somatic cells Produces clones Function Sexual reproduction Occurs in germ cells Produces variable offspring Prophase vs. Prophase I Prophase (Mitosis) Homologous pairs do not interact with each other Prophase I (Meiosis) Homologous pairs become zippered together and crossing over occurs Anaphase, Anaphase I, and Anaphase II Anaphase I (Meiosis) Homologous chromosomes separate from each other Anaphase/Anaphase II (Mitosis/Meiosis) Sister chromatids of a chromosome separate from each other Results of Mitosis and Meiosis Mitosis Two diploid cells produced Each identical to parent Meiosis Four haploid cells produced Differ from parent and one another Meiosis I Prophase I Metaphase I Anaphase I Telophase I Crossing over occurs between homologues. Homologous pairs align randomly. Homologues separate from their partner. Cytoplasm may divide before meiosis II. Fig. 10-11a, p.164 Meiosis II no interphase and no DNA replication between the two nuclear divisions Prophase II Metaphase II Anaphase II Telophase II New spindle All chromosomes Sister chromatids Haploid cells forms in each aligned at the function as moved to nucleus. equator. gametes or opposite spindle spores. poles. Fig. 10-11b, p.164 Mitosis Prophase A spindle forms; tethers chromosomes to spindle poles. Metaphase All chromosomes aligned at the spindle equator. Anaphase Sister chromatids moved to opposite spindle poles. Telophase Two diploid (2n) nuclei form. Fig. 10-11c, p.164
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