PowerPlex Fusion 6C System
®
Performance Packed.
Performance Packed.
The PowerPlex® Fusion 6C System offers rapid-cycling, direct-amplification capabilities
and superior sensitivity along with SE33 and three Y-STR loci for improved mixture
interpretation and unbeatable performance with challenging casework samples.
Additionally, the system is optimized and QC-tested to work at full- or half-volume
reactions with some protocol modifications recommended depending on sample type.
This six-color system allows co-amplification and fluorescent detection of the new 20 CODIS Core Loci: CSF1PO,
D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, vWA, D1S1656,
D2S441, D2S1338, D10S1248, D12S391, D19S433 and D22S1045 (1) plus Amelogenin and DYS391 for gender
discrimination. Penta D, Penta E and SE33 also are included to increase discrimination and allow searching of
databases that contain profiles with these loci. Eleven loci are less than 250bp, making them ideal for working with
degraded samples. Finally, two rapidly mutating Y-STR loci, DYS570 and DYS576, are included in the multiplex to
help determine the number of contributors in a mixture.
D3S1358
D16S539
TH01
D8S1179
DYS391
60 65
100
D1S1656
D2S441
D2S1338
D18S51
vWA
D12S391
D10S1248
D21S11
CSF1PO
D7S820
D19S433
D5S818
Penta E
Penta D
TPOX
SE33
FGA
200
D13S317
D22S1045
DYS576
300
DYS570
400
500
12469MA
A*
*A = Amelogenin
Figure 1. Configuration of the PowerPlex® Fusion 6C System. The PowerPlex® Fusion 6C System allows co-amplification and
fluorescent detection of 27 loci, including all CODIS and ESS loci: D3S1358, D1S1656, D2S441, D10S1248, D13S317, D16S539, D18S51,
D2S1338, CSF1PO, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, SE33, D22S1045, FGA and DYS391, as
well as DYS576, DYS570, Penta E, Penta D and Amelogenin.
1. Hares, D.R. (2015) Selection and implementation of expanded CODIS core loci in the United States. Forensic Sci. Int. Genet. 17, 33–4.
Enhanced Sensitivity
25,000
120
PowerPlex® Fusion 6C System
20,000
100
GlobalFiler® PCR Amplification Kit
80
15,000
60
10,000
40
5,000
Investigator® 24Plex QS Kit
20
2ng
1ng
500pg
250pg
125pg
62.5pg
31.2pg
0
13141MA
0
Alleles Called (%)
Peak Height (RFU)
The PowerPlex® Fusion 6C System reliably produces complete profiles from as little as 125pg of human DNA
and useable, partial profiles from DNA inputs lower than 125pg.
Input DNA Amount
Figure 2. The average peak heights (vertical bars) and percentage of alleles called (diamonds) at varying amounts of 2800M Control DNA.
Error bars represent the standard deviation of the mean from triplicate amplification reactions.
Superior Inhibitor Tolerance
Casework samples are typically laden with inhibitors and can often require laboratories to re-amplify samples and
repeat analyses. The PowerPlex® Fusion 6C System contains a robust buffer system to meet those challenges
head on and increases your chances for success with even the most difficult samples.
120
PowerPlex® Fusion 6C System
GlobalFiler® PCR Amplification Kit
Figure 3. Resistance to PCR inhibitors.
Human genomic DNA (1ng) was amplified with
the indicated kits in the presence of common PCR
inhibitors. Amplified products were separated on an
Applied Biosystem® 3500 Genetic Analyzer (1.2kV,
15-second injection). Percentage of alleles detected
is indicated for each system.
80
60
40
20
0
12560MA
Alleles Called (%)
100
500µM
750µM
Hematin
1,000µM
100ng/µl 200ng/µl 300ng/µl 100ng/µl 300ng/µl 400ng/µl
Humic Acid
Tannic Acid
PowerPlex® Fusion 6C System
Direct-Amplification Capabilities
The PowerPlex Fusion 6C System allows direct
amplification from unwashed FTA® card punches as
well as pretreated nonFTA card punches and typically
used swabs to streamline workflows and expand your
laboratory’s sample throughput.
Extracted DNA
FTA® Punches
NonFTA Punches
Swabs
Extract DNA
from sample
Punch FTA®
card into plate
Punch card into
plate; treat with
PunchSolution™
Reagent
Incubate swab in
SwabSolution™
Reagent
Add up to 15µl
of sample to
reaction
Add PCR
amplification
mix
Add PCR
amplification
mix to
dried punch
Add 2µl of
extract to PCR
amplification
mix
®
11140MC
To maximize the cost effectiveness of STR systems, many
laboratories incorporate the use of half-volume (12.5μl)
amplification reactions in their workflow. The PowerPlex®
Fusion 6C system is designed and QC-tested to work
at full- or half-volume reactions with various protocol
modifications recommended depending on sample type.
Cycling time ~65 minutes for all applications
FTA® Punch Analysis
Buccal Swab Analysis
Buccal Sample on FTA® Card
D1S1656
D3S1358
A…
50
100
150
D2S441
200
D10S1248
250
300
D13S317
350
Swab Sample
Penta E
D3S1358
A…
400
450
500
50
8000
100
D1S1656
150
D2S441
D10S1248
200
250
200
250
300
D13S317
350
Penta E
400
450
500
450
500
450
500
8000
4000
4000
0
0
D16S539
50
100
D18S51
150
D2S1338
200
250
CSF1PO
300
350
Penta D
400
450
D16S539
500
50
8000
100
D18S51
150
D2S1338
CSF1PO
300
350
Penta D
400
8000
4000
4000
0
0
TH01
50
100
vWA
150
D21S11
200
250
D7S820
300
TPOX
D5S818
350
TH01
400
450
500
50
8000
8000
4000
4000
0
100
vWA
150
D21S11
200
D7S820
250
300
250
300
250
300
D5S818
350
TPOX
400
0
D8S1179
50
100
D12S391
150
SE33
D19S433
200
250
300
350
D22S1045
400
450
D8S1179
500
50
8000
8000
4000
4000
0
100
D12S391
D19S433
150
200
150
200
SE33
350
D22S1045
400
450
500
450
500
0
100
150
200
DYS576
250
300
350
DYS391
DYS570
400
450
50
500
8000
4000
0
Figure 4. Direct amplification of two 1.2mm punches from a buccal
sample on an FTA® card. Two 1.2mm punches were added to a plate, and
PCR amplification mix was added to the punches following the recommended
protocol for 12.5µl reactions. Amplified products were separated using an
Applied Biosystems® 3500 Genetic Analyzer (1.2kV, 15-second injection).
100
FGA
DYS576
350
DYS570
400
8000
4000
0
Figure 5. Direct amplification of DNA from buccal swabs. A buccal
swab was pretreated with SwabSolution™ Reagent as recommended in the
SwabSolution™ Kit Technical Manual. Following incubation, 2μl of extract was
added to the PCR amplification mix using the recommended protocol for 12.5µl
reactions. Amplified products were separated using an Applied Biosystems® 3500
Genetic Analyzer (1.2kV, 15-second injection).
12563MA
50
FGA
12562MA
DYS391
PowerPlex® Fusion 6C System
NonFTA® Punch Analysis
Buccal Sample on Bode Buccal DNA Collector™ Device
D1S1656
D3S1358
A…
50
100
150
D2S441
D10S1248
200
250
200
250
300
D13S317
350
Figure 6. Direct amplification of a single 1.2mm punch
from a buccal sample collected using the Bode Buccal DNA
Collector™ device. A punch was pretreated with PunchSolution™
Reagent as recommended in the PunchSolution™ Technical
Manual. Following incubation, PCR amplification mix was added
to the dried punch following the recommended protocol for 12.5µl
reactions. Amplified products were separated using an Applied
Biosystems® 3500 Genetic Analyzer (1.2kV, 15-second injection).
Penta E
400
450
500
450
500
450
500
14000
7000
0
D16S539
50
100
D18S51
150
D2S1338
CSF1PO
300
350
Penta D
400
14000
7000
0
TH01
50
100
vWA
150
D21S11
200
D7S820
TPOX
D5S818
250
300
350
250
300
350
250
300
400
14000
7000
0
D8S1179
50
100
D12S391
SE33
D19S433
150
200
150
200
D22S1045
400
450
500
450
500
14000
7000
0
100
FGA
DYS576
350
DYS570
400
7000
0
13144MA
DYS391
50
14000
Product Information
The PowerPlex® Fusion 6C System is compatible with the Applied Biosystems® 3500 and 3500xL Genetic Analyzers
as well as Applied Biosystems® 3130 and 3130xl Genetic Analyzers with Data Collection Software Version 4.0 with
the DC v4 6-Dye Module v1 License (Life Technologies).
Each PowerPlex® Fusion 6C System contains:
• PowerPlex® Fusion 6C 5X Master Mix
• PowerPlex® Fusion 6C 5X Primer Pair Mix
• 2800M Control DNA, 10ng/μl
• Water, Amplification Grade
• PowerPlex® Fusion 6C Allelic Ladder Mix
• WEN Internal Lane Standard 500
For more information, visit:
www.promega.com/PowerPlexFusion
PowerPlex® Fusion 6C System
Ordering Information
Product
Size
Cat.#
50 (or 100 direct-amp) reactions
DC2705
200 (or 400 direct-amp) reactions
DC2720
5 preps
DG4900
PunchSolution Kit
100 preps
DC9271
SwabSolution Kit
100 preps
DC8271
5X AmpSolution Reagent
100 preps
DM1231
PowerPlex Fusion 6C System
®
PowerPlex 6C Matrix Standard
®
™
™
™
Maxwell, Plexor and PowerPlex are registered trademarks of Promega Corporation. AmpSolution, Differex, DNA IQ, Identity Automation, PowerQuant, PunchSolution, Slicprep
and SwabSolution are trademarks of Promega Corporation.
Applied Biosystems is a registered trademark of Applied Biosystems. Bode Buccal DNA Collector is a trademark of the Bode Technology Group, Inc. FTA is a registered
trademark of Flinders Technologies, Pty, Ltd., and is licensed to Whatman.GlobalFiler is a registered trademark of Life Technologies. Investigator is a registered trademark of
Qiagen GmbH Corp.
Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.
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