Syphilis Reagents for Commercial Assay Development
Recombinant T. pallidum antigens for IgG, IgM and IgA antibody detection
Syphilis is a sexually transmitted disease that can cause very serious complications if left untreated. Older
diagnostic methods (non-treponemal tests) have largely been replaced by newer methods that employ specific
treponemal antigens in novel formats, including rapid POC, ELISA, and chemiluminescence. These new screening
tests detect IgM, IgG and IgA antibodies to T. pallidum using either wild-type or recombinant antigens,
specifically Tp17, Tp15, Tp44.5 (TmpA), and Tp47.
Treponema Pallidum
Spirochetes
Syphilis is a sexually transmitted bacterial infection caused by the
bacterium Treponema pallidum. It is passed from person to person
through direct contact with a syphilis sore and causes a systemic infection
with symptoms that vary depending on the stage of the disease (primary,
secondary, latent, or tertiary). T. pallidum cannot be isolated in culture so
serological diagnostics such as ELISA-based IgG and IgM assays play a
major role in the diagnosis and follow-up of treatment for syphilis.
There are two main categories of serological testing for syphilis: (1)
treponemal tests which are aimed at detecting an antigen or an antibody
to T. pallidum and (2) non-treponemal tests which look for indirect
indications of the infection such as the presence of cardiolipins (a
mitochondrial membrane lipid) which are released when a treponeme
bacteria damages cells. Since non-treponemal tests do not detect the
bacteria directly, they lack specificity and a positive result requires
confirmation by a treponemal-specific test such as an EIA assay that
detects IgG and/or IgM and IgA antibodies to T. pallidum.
Source: academic.pgcc.edu
Copyright © 2006 Pearson Education, Inc. Publishing as Benjamin
Cummings
Several highly immunogenic lipoproteins have been identified as
diagnostic targets throughout all stages of a syphilis infection, including
Tp17, Tp15, Tp44.5 (TmpA), Tp47, Tp41 and Tp35 (TmpC). Specifically,
early immune responses are against TpN47 and some of the flagellar
proteins, followed by Tp15 and Tp17. For this reason, several commercial
tests have been developed using a combination of these immunogenic
antigens and have proven to be highly sensitive and specific for the
diagnosis of an active or latent Syphilis infection.
Meridian Life Science offers a panel of antigens to T. pallidum including a
new chimeric antigen to Tp17/p15/p44.5/p47. It contains all four antigens
expressed in E. coli and has been validated in EIA and rapid format assays.
MERIDIAN SYPHILIS (T. PALLIDUM) RECOMBINANT ANTIGENS FROM E. coli
Chimeric Tp17/p15/p44.5/p47
R01549
Chimeric protein, MW 64 kDa
Tp41
R01529
Full length protein, β-gal tag, MW 153 kDa
Tp15
R8A101
R01531
Full length protein, β-gal tag, MW 60 kDa
Mosaic protein, His tag, MW 19 kDa
Tp47
R8A403
R01568
Full length, β-gal tag, MW 92 kDa
Mosaic protein, His tag, MW ~45kDa
Tp17
R8A201
R01528
Full length protein, β-gal tag, MW 63 kDa
Mosaic protein, β-gal tag, MW 137 kDa
TmpA (Tp44.5)
R8A404
Full length protein, β-gal tag, MW 82 kDa
R01530
A.A. 23-41 and 288-325, GST tag, MW 32 kDa
APPLICATIONS
The T. pallidum genome is 1.14 Mb and encodes a putative 1,041 proteins (Genome Sequencing Project). Different strains
of T. pallidum may express different repertoires of Tp proteins as demonstrated by various immunologic studies (Leader,
B. et al. (2003) Infect. Immun. 71:6054-6057). Commercial tests have been developed using a panel of one or more of the
following immunogenic antigens:
Protein
Tp15
Tp17
Tp41
Tp47
TmpA/Tp44.5
T. Pallidum Genome
Membrane-associated 15 kDa lipoprotein
Membrane-associated 17 kDa lipoprotein
41 kDa homolog of galactose-glucose-binding protein
Membrane-associated 47 kDa lipoprotein with carboxypeptidase properties
Membrane lipoprotein 42 kDa
Recommended for:
The following applications have been tested and validated. Each laboratory should determine an optimum working titer
for use in its particular application. Other applications have not been tested but should not necessarily be excluded.
Catalog
R01549
R01528
R8A201
R01530
R8A404
R01531
R8A101
R01529
R01568
R8A403
Description
Tp17/p15/p44.5/p47
Tp17
Tp17
TmpA
TmpA
Tp15
Tp15
Tp41
Tp47
Tp47
WB
X
X
X
X
X
ELISA
X
X
X
X
X
X
X
X
LF
X
X
X
X
Testing Data:
Chimeric Tp17/p15/p44.5/p47
(R01549)
MW Ladder
FOR RESEARCH OR FURTHER MANUFACTURING USE
Meridian Life Science, Inc.
5171 Wilfong Rd. / Memphis, TN 38134
www.meridianlifescience.com
Orders/Inquiries: [email protected] / Tel: 901.382.8716 / Fax: 901.333.8223