Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B.
This journal is © The Royal Society of Chemistry 2017
Electronic Supplementary Information
Facile preparation of pH/reduction dual-responsive prodrug
nanohydrogels for tumor-specific intracellular triggered
release with enhanced anticancer efficiency
Tingting Zhou, Xu Jia, Xubo Zhao, Jiagen Li, and Peng Liu*
State Key Laboratory of Applied Organic Chemistry and Key Laboratory of
Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of
Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
* Corresponding Author. Tel./Fax: 86 0931 8912582. Email: [email protected].
S1
(a)
(b)
Figure S1. Digital photos of the DOX solution, the dispersions of the mPEG-OAL20.0DOX/Cys prodrug nanohydrogels in pH 7.4 with 10 mM GSH, pH 5.0 with 10 mM
GSH, and water (from left to right) for (a) 0 h and (b) 58 h.
S2
Cumulative drug release (%)
Log of cumulative drug release
1.4
20
mPEG-OAL17.5-DOX/Cys
pH=7.4
15
10
5
y = 0.34341x - 0.94802
R2 = 0.9858
0
0
10
20
30
40
Square root of time
50
1.2 mPEG-OAL17.5-DOX/Cys
0.8
0.6
0.4
0.2
0.0
-0.4
1.0
60
Log of cumulative drug release
Cumulative drug release (%)
mPEG-OAL17.5-DOX/Cys
pH=7.4
30
20
y = 0.80368x - 1.91606
R2 = 0.98724
10
0
0
10
20
30
40
Square root of time
50
60
30
20
y = 0.8926x - 1.52007
R2 = 0.97016
0
3.5
1.6 mPEG-OAL17.5-DOX/Cys
1.4
pH=5.0 with 10 mM GSH
1.2
1.0
0.8
0.6
0.4
y = 0.72982 - 0.78295
R2 = 0.95978
0.2
10
20
30
40
50
60
1.0
1.5
Square root of time
Log of cumulative drug release
16 mPEG-OAL20.0-DOX/Cys
Cumulative drug release (%)
3.0
0.0
0
14 pH=7.4
12
10
8
6
y = 0.25788x +1.35801
R2 = 0.95688
4
2
0
2.0
2.5
Log of time
1.8
mPEG-OAL17.5-DOX/Cys
40 pH=5.0 with 10 mM GSH
Log of cumulative drug release
Cumualtive drug release (%)
1.5
1.8
1.6 mPEG-OAL17.5-DOX/Cys
pH=5.0
1.4
1.2
1.0
0.8
0.6
0.4
y = 0.7202x - 0.80909
0.2
R2 = 0.96762
0.0
-0.2
1.0
1.5
2.0
2.5
3.0
3.5
Log of time
50
10
y = 0.72716x - 1.20702
R2 = 0.94428
-0.2
50
40
pH=7.4
1.0
0
10
20
30
40
Square root of time
50
1.2
1.0
S3
3.0
3.5
mPEG-OAL20.0-DOX/Cys
pH=7.4
0.8
0.6
0.4
y = 0.47124x -0.42764
R2 = 0.97402
0.2
0.0
1.0
60
2.0
2.5
Log of time
1.5
2.0
2.5
Log of time
3.0
3.5
1.8
mPEG-OAL20.0-DOX/Cys
pH=5.0
50
Log of cumulative drug release
Cumulative drug release (%)
60
40
30
20
y = 1.16x -6.12
R2 = 0.97727
10
0
0
10
20
30
40
Square root of time
50
Log of cumulative drug release
Cumulative drug release (%)
50
40
30
20
y = 1.1859x -4.9130
R2 = 0.98408
0
10
20
30
40
Square root of time
1.4
1.2
1.0
0.8
0.6
y = 0.7255x -0.723
R2 = 0.98637
0.4
0.2
1.5
2.0
mPEG-OAL20.0-DOX/Cys
60 pH=5.0 with 10 mM GSH
0
mPEG-OAL20.0-DOX/Cys
pH=5.0
0.0
1.0
60
70
10
1.6
50
60
2.0
2.5
Log of time
3.0
3.5
1.5
mPEG-OAL20.0-DOX/Cys
pH=5.0 with 10 mM GSH
1.0
0.5
y = 0.7599x -0.799
R2 = 0.97
0.0
1.0
1.5
2.0
2.5
Log of time
3.0
3.5
Figure S2. The drug release mechanism fitted with the Higuchi and KorsmeyerPeppas models.
S4