Chapter 1 General Introduction Chapter 1A Coconut The story of the coconut and its presence around the globe is one in which evolution, immigration, trade, cultural practices and the forces of nature, all play a part (Foale, 2003). The term coconut can refer to the entire coconut palm, the seed, or the fruit, which botanically is a drupe or a nut. The coconut palm (Cocos nucifera) is a member of the family Arecaceae (palm family). The name Cocos probably derives from a Portuguese word meaning monkey because its nut, bearing three germinating pores, resembles a monkey face. Its specific name derives from Latin, meaning nut-bearing. In Sanskrit, it is called kalpa vriksha (a mythological tree supposed to grant desires) because of its versatile contribution to mankind that can provide all the necessities of life. This species is known to have several of uses as a source of food, drink, fiber, construction material, charcoal, and oil (used in cooking, pharmaceuticals, industrial applications and biofuels). Coconut is planted over 12 million hectares of land across 93 tropical countries. India is currently one of the top producers of coconuts in the world, with annual production of 11,930,000 tonnes in 2013 (Table 1A.1). 1A.1. Plant and fruit description The coconut tree belongs to the family Arecaceae in the order Palmales. The genus, Cocos, is monospecific, with a pantropical distribution, predominantly in coastal areas between latitude 20 N and S of the equator (Figure 1A.1). The species varies greatly in tree height, fruit shape and size. All coconut cultivars are classified distinctively in two groups: tall and dwarf types. The tall types are referred to as var, typica Nar. while dwarf types as var. nana (Griff.) Nar. The tall varieties are commonly cultivated in all the coconut growing regions of the world. Their fruits are ‘medium to large’ in size. The dwarf 1 varieties are characterized by their short stature and smaller nuts of varying colours, e.g. green, yellow and orange. They are presumed to have originated from the taller palms either through mutation or by inbreeding. The cultivars are usually diploids and the chromosome number is 2n=32 (Ray, 2002). The coconut palm can grow upto 30 meters in height and live for 80 to 100 years. The stem has only one terminal growing point, no axillary vegetative buds and rarely has suckers from the underground portion of the stem. As a monocotyledonous plant, the coconut palm has adventitious root system. The crown of a mature plant usually has about 30 to 40, open, 3 to 6 meter long leaves (fronds), 10 to 14 of which subtend fruit bunches at different stages of development. At each leaf, both staminate (male) and pistillate (female) flowers are borne on an axillary inflorescence. Dwarf forms may begin flowering in 3 years while the tall variety takes 5 to 7 years to flower (Janick and Paull, 2008). The fruit is formed from a flower with three carpels, only one of which develops. The coconut fruit contains only one seed, which is the largest known. The embryo itself is small and located near the stem end. Initially, the endosperm is a liquid containing free nuclei. As the endosperm matures, cell walls form around the nuclei and the endosperm solidifies into an oil rich layer referred to as “coconut meat”. If a mature coconut is left undamaged, the embryo can germinate within the coconut since there is no dormancy period. The base of the embryo swells (referred to as “coconut apple”) and eventually digests the endosperm. The germinating seedling eventually extends through one of the eyes (Pooja, 2010). A coconut tree can produce up to 75 melon-sized fruits per year. Each fruit can weigh 1- 2 kg (depending on the variety) and is composed of 35% husk, 2 12% shell, 22% kernel and 25% (w/w) water. The coconut is the entire fruit, and botanically it is a drupe, consisting of pericarp and mesocarp (husk), endocarp (shell) and testa enveloping the mature endosperm (kernel) which, importantly, has a central cavity (air space) containing residual liquid endosperm (water). Figure 1A.2 gives a diagrammatic representation of mature coconut fruit and its cross section. The large size and heavy weight of the seed nut prevent dissemination by animals or birds but the fibrous husk and the air space give it the necessary buoyancy for dispersal by floating. The fruit takes ∼11 to14 months to develop after the receptive female flower is successfully pollinated and before the growing point of the embryo penetrates the germ pore (Harries, 2012). 1A.2. Brief history Coconuts have been featured in the Hindu epic stories of the Ramayana and Mahabharata (Menon and Pandalai, 1958). Early Sanskrit writings from the 4th century B.C as well as Tamil literature dating from the 1st-4th century AD mention the coconut tree. The fruit has a special mention in the Mahavamsa texts of Sri Lanka too, dating back to the 1st century B.C. The coconut was mentioned by an Egyptian monk named Cosmos Indicopleusters in 545 A.D. when he visited India and Ceylon. Cosmos describes the coconut as ‘the great nut of India’ in his “Topographia Christiana”. The first detailed description of the palm in western literature was provided by the Italian explorer Ludovico di Varthe in his “Itinerio” of 1510 (Grimwood et al., 1975). A native of the old world tropics, coconut’s domestication history and its population genetic structure relate to human dispersal patterns. Two geographical origins of coconut cultivation: islands of Southeast Asia and 3 southern margins of the Indian subcontinent have been proposed (Gunn et al., 2011). More than 2000 years ago, people took the coconut with them across the Indian Ocean to Madagascar, from where it journeyed on to East Africa. In more recent times, Portuguese mariners, beginning with Vasco da Gama in 1498, took the coconut from India and East Africa to the tropical eastern Atlantic. From the Portuguese stronghold on the Cape Verde Islands (off the coast of Senegal in West Africa), coconuts were taken in two directions. Westwards, they were a source of food and drink on slave-trading ships bound for Cuba and other islands, with those fruits remaining on arrival being planted in the New World as a foundation food source. Eastwards, they were dispersed from the Cape Verde Islands to the coast of West Africa, from Senegal all the way to Angola. It was then dispersed to the islands of the Caribbean, on the Caribbean coast of Mexico and Central and South America, and along the coast of West Africa (Foale, 2003). It thereby became, and it remains, the most widespread and widely used palm in the world. 1A.3. Global and national scenario Coconut is grown in more than 93 countries around the world in an area of about 12.17 million hectares, producing more than 60 million tonnes of coconut annually. It provides food security and livelihood opportunities to more than 20 million people around globe. India, Indonesia, Brazil, Philippines, Thailand and Sri Lanka are the major coconut growing countries, contributing to 78% of world production. India fares better than many other countries with respect to production and productivity; and therefore, it enjoys a comparative advantage (Lathika and Kumar, 2009). Table 1A.1 gives a list of the top 5 coconut producing countries in world with respect to production and 4 percentage of world’s total coconut production. The coconut palm provides a substantial export income for many tropical countries, as well as food and drink for local consumption besides fuel and shelter. The Asian and Pacific Coconut Community (APCC) is an intergovernmental organization established in 1969 under the aegis of the United Nations Economic and Social Commission for Asia and the Pacific (UN-ESCAP). The APCC has 18 coconut producing member countries accounting for over 90% of world coconut production and exports of coconut products. The APCC member countries include: Federated States of Micronesia, Fiji, India, Indonesia, Kiribati, Malaysia, Marshall Islands, Papua New Guinea, Philippines, Samoa, Solomon Islands, Sri Lanka, Thailand, Tonga, Vanuatu, and Vietnam. Jamaica and Kenya are associate member countries of the APCC. India is bestowed with most congenial agro-climatic conditions, diverse soil types and abundant water resources required for coconut cultivation. The statistics released by the Horticulture Division of Ministry of Agriculture, India was in Metric tonnes (MT) and the production figure was 14 million MT which in terms of whole nuts is equivalent to 21892 million nuts for the year 2011-12. In India, there is a distinct difference in pattern of distribution of coconut. The four southern states; Kerala, Karnataka, Tamil Nadu and Andhra Pradesh are the main coconut growing areas in the country which together account for 90% of area under coconut cultivation and 93% in coconut production. Major portion of coconut comes from the West Coast comprising of Kerala, Goa, Karnataka and Maharashtra followed by the East Coast of Andhra Pradesh, Orissa, Tamil Nadu and Pondicherry. The Islands of Andaman & Nicobar, 5 Lakshadweep and the coastal regions of Gujarat are the other traditional coconut areas. In non-traditional states like Assam, Tripura, Nagaland, Manipur, Mizoram, Meghalaya and Arunachal Pradesh also coconut cultivation has gained momentum. Table 1A.2 gives state wise distribution of coconut in the year 2011-12. Kerala ranks first in coconut production but the growth in real estate, speedier urbanization and faster substitution of coconut area for more remunerative crops like rubber has resulted in decelerating growth in area under coconut. Karnataka and Tamil Nadu, together, account for more than half of the country’s production. About 95 per cent of the edible copra produced in the country is Karnataka’s share. In Tamil Nadu, coconut cultivation and allied industries have now become the main source of livelihood and employment security for a large portion of the population in the state. Coconut is extensively grown in three union territories of India, namely, Andaman & Nicobar Islands, Lakshadweep and Pondicherry. Coconut in Lakshadweep is cultivated using organic farming methods and this small island occupies the number one position in the country in terms of productivity of coconut. There has been perceptible increase in area under coconut cultivation in India since the last six decades. The formation of Coconut Development Board (CDB) with subsequent formation of coconut mission and its field level network in the length and breadth of the country was instrumental for the expansion of the crop nationwide giving a national image to the crop. Besides Coconut Development Board, State department of Agriculture, Horticulture, State Agricultural Universities, Research Institutes and other related organizations 6 in government and private sector contribute to the overall growth of Indian coconut sector (Gopalakrishnan, 2013). Though India has become the global leader in coconut production, it has not yet achieved much in the diversification of the produce. Coconut processing is confined only to copra production, oil extraction, production of desiccated coconut and manufacture of coir and coir products. About 60% of coconut is used for edible and religious purposes, 35% as milling copra for oil extraction, approximately 3.5% for tender coconut water and the remaining processed as desiccated coconut (NIIR Board of Consultants and Engineers, 2006). Other countries like the Philippines, Indonesia and Vietnam have been adding value to this agricultural product tremendously using the state-of-the-art technologies in the food processing industry. Failure of the Indian coconut industry to diversify from its traditional sector is the main reason attributed to the slow pace of development of coconut industry in India. Traditionally, coconut was grown on a large scale for the production of edible oil. It served as an ingredient for various industrial applications too. The changed food habits and availability of other cheaper edible oils both in the edible and industrial sectors have resulted in a drastic decline in the use of coconut oil in these areas. During the last few years, on account of heavy imports of cheaper vegetable oil, especially of the Palmolein, the price of coconut oil has been put low despite the large-scale price support operations undertaken. The diversification of coconut derived products and value addition could only help the coconut growers in getting remunerative returns for the produce. Drastic change in this situation is the need of the hour and can be achieved through processing and export of diverse coconut products. 7 Table 1A.1: Top five coconut producing countries in 2013. Rank Country Coconut production (tonnes) Percentage of world’s total coconut production* (%) 1 Indonesia 18,300,000 29.5 2 Philippines 15,353,200 24.8 3 India 11,930,000 19.3 4 Brazil 2,820,468 4.5 5 Sri Lanka 2,200,000 3.6 * World production of coconut in 2013 was 61,965,165 tonnes Compiled from http://faostat.fao.org 8 Table 1A.2: All India estimates of area under cultivation, production and productivity of coconut during 2011-12 Sl no. State /Union Territory Area Production Productivity ('000 Hectares) (000 metric ton)* (Kilogram/ha) 1 Andaman and Nicobar Islands 21.80 72.30 5184 2 Andhra Pradesh 142.00 1270.00 13976 3 Assam 18.80 101.00 8397 4 Chhattisgarh 0.80 6.30 12309 5 Goa 25.70 88.70 5394 6 Gujarat 20.90 217.90 16296 7 Karnataka 506.80 3770.00 11627 8 Kerala 766.00 3973.00 8109 9 Lakshadweep 2.70 40.00 23156 10 Maharashtra 21.00 120.00 8931 11 Nagaland 0.90 0.30 521 12 Orissa 53.90 258.00 7482 13 Puducherry 2.10 20.00 14886 14 Tamil Nadu 420.70 3692.00 13717 15 Tripura 5.90 8.60 2278 16 West Bengal 29.10 367.50 19739 2039.10 14006.50 10736 All India Source: Advisor, Horticulture Division, Ministry of Agriculture, Govt. of India. (http://coconutboard.nic.in/stat) * 1563 nuts - 1 metric ton 9 Figure 1A.1: The range of the natural habitat of the coconut palm tree delineated by the red line. Source: http://en.wikipedia.org/wiki/Coconut 10 Figure 1A.2: Diagrammatic representation of mature coconut fruit and its cross section. 11 Chapter 1B Coconut Products and Processing 1B.1. Coconut products There is a saying, “He who plants a coconut tree, plants food and drink, vessels and clothing, a habitation for himself and a heritage for his children” (Pooja, 2010). A plethora of information is available about coconut products and processing (Dendy and Grimwood, 1972; Grimwood et al., 1975; Hazelman, 1997; NIIR Board of Consultants and Engineers, 2006; Philippine Coconut Authority, 1997; United Nations Industrial Development Organization and Ranasinghe, 1980; Woodroof, 1979). The coconut tree is of 100% utility. The roots can be used for extracting dye stuff besides medicinal purposes. The trunk yields handy and durable wood which can be used to make lumber, various pieces of furniture, paper pulp and art or show pieces. The leaves produce good quality of paper pulp and used for making brooms, hats, mats, fruit trays, hand fans, midrib decors, lamp shades, bags and often used also as roof materials, creating fences and kindling fires. Coconut inflorescence, when cut, yields a liquid called Neera. Palm sugar or jaggery is produced by carefully evaporating this liquid in open pots. Neera ferments, usually spontaneously, to give palm wine or toddy which can be distilled to yield liquor known as arrack (Grimwood et al., 1975). The coconut (fruit) itself has numerous uses. The liquid endosperm in the young and fresh coconut makes a refreshing natural beverage. There has been increasing scientific evidence that supports the role of coconut water in health and medicinal applications. Coconut water is used as a growth supplement in plant tissue culture/micropropagation. The wide applications of coconut water can be justified by its unique chemical composition of minerals, vitamins, amino acids, phytohormones and sugars (Yong et al., 2009). It was 12 shown that this sterile liquid can even be used as a short-term intravenous hydration fluid (Campbell-Falck et al., 2000). The thin coconut solid endosperm (soft, jelly-like) of young coconut can be consumed as such or used in culinary especially desserts. The energy-rich kernel of mature coconut is an indispensable ingredient in most of the tropical cuisine. It is a good source of oil, fibre and protein, and acts as a natural laxative. Coconut meat can be processed to yield coconut milk, copra, oil and many other commercial products which are discussed extensively in section 1B.2. The hard shell, or endocarp, is used as fuel, food packaging material (icecream cups) and ornaments. A fine grade of charcoal can be produced which can be used as fuel or for purposes such as ironing and smokeless fires in restaurants. Shells can be also for preparation of fine grade activated carbon. The fibrous husk contains an important fibre, commercially known as coir. Coir is widely used in making mats, ropes, nets and other artefacts. Coir dust, the fluffy particles obtained during separation of coir fibres from husk, can be used as filler in soil to increase its moisture retention and as an insulating material owing to its low thermal conductivity (Grimwood et al., 1975). 1B.2. Coconut processing Coconut products have experienced astonishing growth globally over the past several years. A wide range of coconut products are internationally traded. There are more than 50 unprocessed, semiprocessed or processed coconut products entering the international markets in small and big quantities. The major products exported from coconut producing countries are copra and coconut oil. Other exports which have a significant volume are desiccated coconut, copra meal, cocochemicals (fatty acids, fatty alcohols, methyl 13 ethers), shell charcoal and activated carbon, fibre products, coconut cream, milk, powder and nata de coco. Return on growth of the coconut products’ industry depends on how coconuts are processed and handled from the farm to packaged goods. This section deals extensively with processing of commercially available edible coconut products. 1B.2.1. Copra Copra is the dried endosperm (nutritive tissue) of the coconut. More than 50 percent of the world’s coconut produce is processed into copra. It can be broadly classified as edible or milling types. Edible grade of copra is consumed as a dry fruit while milling copra is used to expel oil. The quality parameters of copra for grading for different uses are specified in IndianStandard, IS: 6220-1971, which prescribes the methods of grading and the requirements of copra for extraction of oil and for table use, together with methods of sampling and tests. The 3 types of copra are defined as: type 1 (grades 1, 2 and 3), i.e. ball copra for table purpose; type 2 (grades 1 and 2), i.e. cup copra for table purpose and type 3 (grades 1, 2 and 3), i.e. milling copra for oil extraction. Edible copra constitutes a very small percentage of the total production but there could be better prospects for export market owing to the increasing demand for edible copra by people living in the colder climatic regions. Edible copra is of two different kinds namely, cup copra and ball copra. Cup copra is usually processed in the copra kiln using fully-matured coconuts as raw material in which coconuts are split-opened into two halves and loaded onto the copra bed after a pre-drying (solar) in a cemented open yard for six to seven hours. Although pre-drying of the coconut halves in an open yard 14 contributes to the fuel saving, it increases the chances of microbial contamination in the end product. There are concerns with regard to the deposition of smoke and lack of uniformity in the dehydration in the kiln drying process which eventually affect the quality of the edible copra in terms of discoloration, under drying, scorching or case-hardening. Ball copra, in essence, is a substance formed within a fully matured whole nut due to natural dehydration of coconut water. The fully mature (10-12 months), unhusked nuts are stored for many months during which the coconut water slowly gets absorbed and the kernel dries out. The dried kernel slowly detaches from the coconut shell leading the formation of ball copra. This stage can be well-recognized with a sound inside the cavity upon shaking. At this stage, nut is dehusked and the shell is carefully removed to separate the whole dry kernel. The ball copra has a soft texture and oily and sweet taste. With increasing use of technology, ball copra formation could be expedited through a method of kiln drying, where the time duration for ball copra formation could be reduced from seven months to about two months (Marikkar et al., 2009). Coconut is one of the most widely used oil seed containing about 35% fat and 50% moisture (wet basis). Moisture content of coconut is required to be reduced to less than 7% by drying to reduce the weight, prevent microbiological deterioration and increase oil content before expelling of oil. Traditional methods include solar drying, smoke drying and drying using kiln. Copra manufacture solely by solar drying is effective only in regions with long periods of sunny clear skies, high mid-day temperatures (30–35°C in the shade) and low humidity air (60–70% RH). Solar drying involves spreading of 15 split coconuts on mats, cement floors, roof tops or even on soil along the roadsides so as to expose to solar intensity until the completion of drying. Exposure to direct sun light leads to heating of coconut pieces without regulation which destroys colour, vitamins and flavour giving rise to low quality produce. Solar drying also suffers from high product losses due to inadequate drying, fungal growth, encroachment of insects, birds and rodents. Smoke drying or curing involves drying of coconuts over open fire where the resultant combustion gases come in contact with coconut meat. It is a very fast and efficient dehydrating method but as smoke comes in direct contact with the coconut, there is formation of polycyclic aromatic hydrocarbons and increase in acid content, yielding poor quality of copra. Kiln drying involves drying using commercially produced dryers or Kilns. The main components of the kiln are drying chamber, roofing, grill, heat spreader, fire container and fuel tunnel. The kilns used to dry coconut kernels vary from country to country. The quality of the copra produced varies widely depending on the type and operational conditions of the kilns. Usually a combination of preliminary solar drying followed by kiln drying is used. Recently, a biomass fired drier that yields high quality copra was designed, fabricated and tested for drying coconut (Swain, 2013). Although copra is not considered as a very high value product and application of sophisticated dryers may not be appropriate, efforts have been made to design and develop solar tunnel dryer (Kulanthaisami et al., 2009) and forced convection solar dryer (Mohanraj and Chandrasekar, 2008). 16 1B.2.2. Coconut oil Coconut oil is an edible oil that has been consumed in tropical countries for thousands of years and currently finds various applications in food, medicine, and industry. It has a long shelf life and a melting point of about 76 °F (~24°C) (Gopala Krishna et al., 2010). A negative campaign against saturated fats in general, and the tropical oils in particular, led to abandoning coconut oil manufacture and consumption to a large extent in the past few decades. With recent peer-reviewed research (Table 1B.1) as well as publishing coconut oil testimonials’ (showing how coconut oil has changed peoples’ lives), coconut oil has gained popularity and reputation as one of the healthiest oils with versatile health benefits. The nutritional value of coconut oil is presented in Table 1B.2. Industrial applications of coconut oil involve utilization as feedstock for biodiesel to be used as a diesel engine fuel (Kumar et al., 2010), engine lubricant (Jayadas and Nair, 2006) and as transformer oil (Abeysundara et al., 2001). Different types of coconut oil (edible) are available, namely, virgin coconut oil from wet coconuts (unrefined grade); coconut oil from copra (refined/unrefined grades); and coconut oil by solvent extraction method (refined, from coconut expeller cake). Coconut oil is obtained through either "dry" or "wet" processing. In dry processing, oil is expelled from copra by pressing (using expellers) or by solvent extraction from spent cakes. Commercially, oil from copra is expelled using rotary ghanis, hydraulic presses or expellers. This "crude" coconut oil may not be suitable for direct consumption as it may contain contaminants and must be refined with further heating and filtering. Refined, bleached, and deodorized (RBD) coconut oil is widely available and 17 used for cooking, commercial food processing, and cosmetic, industrial, and pharmaceutical purposes. But it lacks the typical coconut oil aroma and has a bland taste due to the refining processes. RBD coconut oil can be processed further into partially or fully hydrogenated oil to increase its melting point (upto 40C). Wet processing involves extraction of coconut oil from fresh mature coconut rather than dried copra. When oil is extracted without employing heat, shear, chemicals or refining it is known as virgin coconut oil. Different methods, quality of oil, advantages and limitations are described in Table 1B.3. Virgin coconut oil is claimed to have more health benefits compared to coconut oil expelled from copra (Nevin and Rajamohan, 2006). 1B.2.3. Desiccated coconut Desiccated coconut, also known as coconut powder, is the dry form of grated fresh coconut. It has become a mass consumption item due to its availability round the year, long shelf life, reduction in wastage, convenient to transport and freedom to the consumers to buy the required quantity. Desiccated coconut has a great export value and its main consumers are confectionary and biscuit industry. The manufacturing process is simple and wellestablished. Fully grown and matured coconuts of around one year are stored with husk for a few days to facilitate absorption of mature coconut water into the kernel. After de-husking, their shells are removed and the testa is scrapped off (known as paring), followed by washing and disintegration. The grating is then tray dried at about 70-80C until moisture content reduces to ~3%. The dry powder is then passed through vibratory screen with different mesh sizes and packaged. Desiccated coconut is available in different grades – fine, medium and coarse, as well as fancy cuts including threads, strips, 18 chips, slices and shreds (Grimwood et al., 1975). On an average, processing of 100 coconuts gives around 12-13 kgs of desiccated coconut. The nutritional value of desiccated coconut is presented in Table 1B.2. 1B.2.4. Coconut milk and related products Coconut milk is a generic term for the aqueous emulsion expelled from wet solid coconut endosperm and it plays an important role in the cuisines of South East Asia as well as other parts of the world. It is a rich source of fat, carbohydrates and minerals (Table 1B.2). In home preparations, coconut milk is squeezed by hand from the freshly grated coconut (wrapped within a cheese cloth). This process is usually repeated twice or thrice by adding water at room temperature, each time obtaining a more dilute milk. The liquid emulsions expelled from the wet coconut endosperm are classified commercially into (1) concentrated coconut cream; (2) coconut cream concentrate (undiluted coconut milk); (3) coconut cream; (4) coconut milk and (5) light coconut milk based on minimum coconut fat and non-fat solids, and maximum water content (Seow and Gwee, 1997). The production of coconut milk begins with deshelling and paring of fully mature coconuts. It is recommended to wash the meat in water containing 100 ppm H2O2, followed by blanching at 80C for 10 min to reduce the initial microbial load and to inactivate lipase before disintegration/grating using a hammer mill (Arumughan et al., 1993). After disintegration, the coconut meat is pressed using a continuous screw press with or without addition of water to give coconut milk. This may be then filtered or centrifuged at low speed and homogenised prior to packaging in cans and sterilized in retort (Chiewchan et al., 2006). Most coconut milk processed in this manner and sold commercially 19 in cans will naturally separate into aqueous and cream phases during storage and transportation. The two phases can easily be mixed back together by stirring or shaking the can. This phase separation can be retarded by using emulsifiers and stabilizers such a polyoxyethylene sorbitan monostearate, guar gum, xanthan gum, gellan gum and sodium carboxymethyl cellulose. Sodium benzoate is usually used as a preservative. Coconut milk can be cooled to sludge, canned and frozen to retain its flavour and freshness for over a year when stored at -23C (Grimwood et al., 1975). Spray dried coconut milk powder is now available in convenient and ready to use packs with same freshness of a fresh coconut milk. This can be used on reconstitution with water in place of fresh coconut milk for food preparations/beverages in households and food industries. Its manufacturing process involves homogenizing coconut milk and addition of maltodextrin, sodium caseinate and other additives. This mixture is then spray dried into a fine powder using a spray drier. The product can be packaged in various sizes as per customer requirement. By decreasing water content and water activity, spray dried coconut milk powder ensures microbiological stability, avoids the risk of chemical and biological degradations, reduces the storage and transport costs and finally obtain a product with specific properties like instantaneous solubility. Council of Scientific and Industrial Research - Central Food Technological Research Institute (CSIR-CFTRI), Mysore with the financial assistance of the Coconut Development Board has developed the technology for spray dried coconut milk powder which was made available to entrepreneurs. Spray dried coconut milk powder is produced on a commercial scale in the Philippines, Indonesia, Malaysia, Thailand and India. The major 20 markets for coconut milk and coconut milk powder are European countries; like United Kingdom, Netherlands, Germany, France; USA, Mexico, Canada, UAE, Australia, Japan, Korea, Malaysia and South Africa (Muralidharan and Jayashree, 2011) 1B.2.5. Tender coconut water The tender coconut water, technically the liquid endosperm present in young green coconut (7-8 months old), is the most nutritious wholesome beverage that the nature has provided to mankind. Tender coconut water is often sold by street vendors who cut the tender coconut open in front of customers in tropical countries. Coconut water can be now packaged in cans, tetra packs or plastic bottles (sometimes with coconut pulp or coconut jelly included) with or without carbonation. After the tender coconuts are harvested, they are thoroughly water washed, then sanitized by transferring to a 1% bleach solution for at least 15 minutes. The nuts are cut and the coconut water is immediately filtered through a coarse filter to remove solids and particulates. The water is then transferred to a sterile refrigerated tank and cooled to 4-6C to avoid fermentation and enzymatic deterioration during further processing. A clarifying resin such as polyvinylpolypyrrolidone (PVPP) is added to reduce the level of polyphenols and tannins and to improve the stability of the final product. The resin is then removed by a coarse filtration, following which the coconut water is transferred to a pressurized holding tank. Nitrogen gas is then used to push the coconut water through sterile microfilters into a sterile holding tank. The sterile coconut water is then aseptically bottled (Rolle, 2007). The Coconut Development Board in collaboration with the Defence Food Research Laboratory (DFRL), Mysore has developed a technology for 21 preservation and packing of tender coconut water in pouches and aluminium cans with retention of its flavour for a period of three months under ambient conditions and six months under refrigerated conditions. In Thailand, young coconuts are trimmed, treated and packaged with opener, straw and spoon. These are commercially produced and marketed (even exported to countries like Australia, Europe, Japan, USA, Taiwan, Hong Kong etc.). The shelf life of the processed young coconut is 45 days at 3-6C or 3 weeks at 7–10C (Muralidharan and Jayashree, 2011). Tender coconut water serves as a mineral drink with therapeutic properties that help in regaining the vitality of the human body. Coconut water has recently been popular among athletes, health freaks and urbanites in many developed countries due to its high potassium and mineral content (Table 1B.2). Soft drink giants like Coca Cola and PepsiCo have acquired top two brands of tender coconut water, Zico and O.N.E, respectively. United Kingdom, Netherlands, Canada, Mexico, UAE, Japan, Korea and Australia are the major importers of tender coconut water. Tender coconut water contains only 3-6% solids and the remaining is water. Removal of the water, while preserving the nutrients, aids in transporting the goodness of tender coconut water across the world. This is achieved by either spray drying or freeze dying tender coconut water with or without additives. This coconut water powder is reconstitutable in water to make a hydrating drink or can be added in other recipes like smoothies and desserts. 1B.2.6. Mature coconut water As the coconut matures, the solid endosperm thickens while the amount of liquid endosperm reduces. At the age of 10-12 months, when the coconut is 22 cracked open, this sweet liquid can be collected and consumed directly as it is sterile and contains sugars and vitamins. Mature coconut water is a major byproduct of the copra and desiccated coconut industry, which often causes disposal problems. It is generally fed to pigs and cattle when fresh but usually goes to waste. It can be collected and used for different applications such as growth media for micro-organisms and plant tissue culture (Prades et al., 2012). Mature coconut water is also used for the manufacture of Nata-decoco and vinegar. 1B.2.7. Other products A tremendous scope exists for use of coconut in a variety of food products. The development of cottage industries to produce such products is recommended to increase incomes of coconut growers. One such product is Nata de coco, which is a chewy, translucent, jelly-like foodstuff produced by the fermentation of coconut water. Originating in the Philippines, Nata de coco is most commonly sweetened as a candy or dessert. Nata-de-coco is produced from filtered coconut water to which glacial acetic acid and sugar are added in specific quantities. The mixture is boiled for ten minutes and cooled. A culture solution is added and allowed to ferment for about three weeks. The Acetobacter xylinum bacteria produce cellulosic jelly substance on the surface of this coconut water. This jelly, or Nata-decoco, is harvested and washed to eliminate traces of acid and diced into cubes. These cubes are boiled in flavoured sugar solution and packaged in glass jars or retortable pouches, sterilized and sealed (Lusas et al., 1989). Nata de coco is regarded for its high dietary fibre, and its low fat and zero cholesterol content. 23 Coconut vinegar is produced with matured coconut water by addition of sugar to increase its sugar concentration to 15%. The solution undergoes fermentation for about five days in the presence of Saccharomyces cerevisiae (yeast). The clear liquid from the alcoholic fermentation is inoculated with 10% vinegar containing Acetobacter bacteria. The acetic acid fermentation takes about 1 month. The vinegar is then filtered, sterilised and bottled (Steinkraus, 1995). Value added products like mature coconut-water concentrate (i.e. coconut honey), tender coconut beverage (i.e. coconut lassi), coconut whey protein from aqueous extract after the recovery of coconut milk, coconut spread based on mature coconut-water concentrate and dietary fiber, coconut soufflé and coconut chutney have been developed at CSIR-Central Food Technological Research Institute, Mysore with the financial support of Coconut Development Board, India (Raghavarao et al., 2011). Numerous other traditional and new processed foods containing coconut are being prepared and tested for their acceptability, quality and potential for commercial manufacture. These include cakes containing 10-40% coconut flour, coconut candy, “tokua”, soya curd containing 50% coconut milk and 50% soya milk, soya tofu containing coconut cream, “coco spread”, containing coconut milk, brown sugar, citric acid and legume flour, “tahu”, a snack based on coconut and soya milk, etc. (Salunkhe and Kadam, 1995). Thus it is rightly said "The coconut palm is alone sufficient to build, rig and freight a ship with bread, wine, water, oil, vinegar, sugar and other commodities". 24 Table 1B.1: Health benefits of coconut oil Sl. No. 1. Aspect Details Comments Principal oil components Fatty acid composition: ~50% lauric acid, 70% medium-chain fatty acids (MCFAs). Highest proportion of medium-chain components of any oil. High melting point; solid below 24°C. Most stable of all the 92% saturated fatty acids cooking oils. Saturated (Gopala Krishna et al., fats have no double 2010). bonds at risk of oxidation therefore giving outstanding stability in storage and use. 2. Uptake path in human body MCFAs are directly absorbed from the intestine and sent straight to the liver to be rapidly metabolized for energy production and do not participate in the biosynthesis and transport of cholesterol (DebMandal and Mandal, 2011). All long-chain fats (saturated or not) are carried directly to lipid deposits, accumulating as body fat. 3. Reduces cholesterol and triglyceride levels Virgin coconut oil has antioxidant activities and does not adversely alter serum lipid levels. Sperm count, motility and serum testosterone levels are also reported to increase. These antioxidants offer protective effects on alcohol-induced oxidative stress in rats (Dosumu et al., 2012). Consumption of virgin coconut oil reduces total cholesterol, triglycerides, phospholipids, LDL, and VLDL cholesterol levels and increased HDL cholesterol in serum and tissues (Nevin and Rajamohan, 2004). Erroneous perception that coconut oil raises cholesterol due to selective tests with flawed diets. 4. Slows the progression of Alzheimer’s disease Medium chain triglycerides from coconut oil given to Alzheimer’s patients led to significant increase in levels of the ketone body beta-hydroxybutyrate (beta-OHB). Higher ketone Major advantage: the saturated fat of coconut oil provides is its ability to provide the brain with an alternate source of energy in ketones. 25 values were associated with greater improvement in paragraph recall with MCT treatment relative to placebo across all subjects (Nafar and Mearow, 2014; Reger et al., 2004). 5. Reduces obesity Dietary supplementation with coconut oil leads to increase in High Density Lipid (HDL) and lowers Low Density Lipid (LDL): HDL ratio (Assunção et al., 2009). Coconut oil does not cause dyslipidemia and seems to promote a reduction in abdominal obesity. 6. Antibiotic effect of mediumchain fatty acids on pathogens Derivatives of lauric and capric oils suppress bacterial, fungal and viral pathogens of humans, including HIV (German and Dillard, 2004). Coconut oil is widely used in treating skin wounds. HIV suppression is promising, and study continues (Dayrit, 2000) 26 Table 1B.2: Nutritional value (per 100 g) of coconut oil, desiccated coconut, coconut milk and coconut water. Sl. no Nutrient Coconut oil Desiccated coconut Coconut milk Coconut water 1. Water (g) 0.00 3.00 67.62 94.99 2. Energy (kcal) 862 660 230 19 3. Protein (g) 0.00 6.88 2.29 0.72 4. Total lipid (fat) (g) 100.00 64.53 23.84 0.20 5. Carbohydrate, by difference (g) 0.00 23.65 5.54 3.71 6. Fiber, total dietary (g) 0.0 16.3 2.2 1.1 7. Sugars, total (g) 0.00 7.35 3.34 2.61 8. Calcium, Ca (mg) 0 26 16 24 9. Iron, Fe (mg) 0.04 3.32 1.64 0.29 10. Magnesium, Mg (mg) 0 90 37 25 11. Phosphorus, P (mg) 0 206 100 20 12. Potassium, K (mg) 0 543 263 250 13. Sodium, Na (mg) 0 37 15 105 14. Zinc, Zn (mg) 0.00 2.01 0.67 0.10 15. Vitamin C, total ascorbic acid (mg) 0.0 1.5 2.8 2.4 16. Thiamin (mg) 0.000 0.060 0.026 0.030 17. Riboflavin (mg) 0.000 0.100 0.000 0.057 18. Niacin (mg) 0.000 0.603 0.760 0.080 19. Vitamin B-6 (mg) 0.000 0.300 0.033 0.032 20. Folate, DFE (µg) 0 9 16 3 21. Vitamin B-12 (µg) 0.00 0.00 0.00 0.00 22. Vitamin A, RAE (µg) 0 0 0 0 23. Vitamin A, IU (IU) 0 0 0 0 24. Vitamin E (alpha-tocopherol) (mg) 0.09 0.44 0.15 0.00 25. Vitamin D (D2 + D3) (µg) 0.0 0.0 0.0 0.0 0 0 0 0 27. Vitamin K (phylloquinone) (µg) 0.5 0.3 0.1 0.0 28. Fatty acids, total saturated (g) 86.500 57.218 21.140 0.176 29. Fatty acids, total monounsaturated (g) 5.800 2.745 1.014 0.008 30. Fatty acids, total polyunsaturated (g) 1.800 0.706 0.261 0.002 31. Cholesterol (mg) 0 0 0 0 32. Caffeine (mg) 0 0 0 0 26. Vitamin D (IU) Source: Compiled from Agricultural Research Service, United States Department of Agriculture, National Nutrient Database for Standard Reference, Release 26 (http://ndb.nal.usda.gov/ndb/foods) 27 Table 1B.3: Comparative analysis of different processes for producing VCO Sl. No. Type of Process Quality of Oil and Advantages Recovery Limitations Fresh-dry processes 1. High pressure expeller method FFA (Free Fatty Acid): 0.05–0.08% MC: 0.07–0.1% Wet milling route Colour: water-clear Oil recovery: 60 kg Moisture per 100 kg of dried Content (MC) of milled kernel; 31 kg dried kernel for per 100 kg of fresh extraction milled coconut should be at 3kernel with testa 4% (based on 50% initial MC of fresh kernel) Highest extraction efficiency 2. High pressure expeller method Desiccated coconut route MC of dried kernel for extraction should be at 34% 3. High pressure expeller method Grated nut route MC of dried kernel for extraction should be at 3– 4% FFA: 0.05–0.08% MC: 0.0–0.1% Colour: water-clear Oil recovery: 58 kg per 100 kg of desiccated coconut ; 30 kg/100 kg of fresh pared, ground kernel (based on 50% initial MC of fresh kernel) FFA: 0.05–0.08% MC: 0.07–0.1% Colour: water-white Oil recovery: 30 kg per 100 kg of fresh grated kernel (based on 50% initial MC of kernel) and Produces full-protein, medium- fat coconut flakes with testa as a co-product which can be further processed into coconut flour or sold as an aflatoxin-free animal feed ingredient. Long shelf-life of oil – 1 year and above. Uses mechanical type of equipment to produce the oil. Applicable in a village scale plant operation (5,000+ nuts/day). Produces full-protein, medium-fat coconut flour without testa as a coproduct. Long shelf-life of oil – 1 year and above. Uses mechanical type of equipment to produce the oil. More appropriate to be used in tandem with an existing desiccated coconut processing plant. Produces full-protein, medium-fat coconut flour without testa as a coproduct. Long shelf-life of oil – 1 year and above. Uses mechanical type of equipment to produce the oil. Applicable in a village scale 28 4. Low pressure expelling method MC of dried kernel for extraction should be within the range of 10– 12%. 5. Centrifuge method MC of dried kernel prior to micropulverisation at 5% FFA: 0.05–0.08% MC: 0.1% and below Colour – water-clear Oil recovery - 60 kg per 100 kg of dried ground kernel without testa; 31 kg per 100 kg of fresh pared kernel (based on 50% MC of fresh kernel) Second highest oil extraction efficiency. plant operation (5,000+ nuts/day). Uses manually operated equipment to produce the oil. Produces a semi-dry coconut residue that has to be further dried or processed to have market value. Shelf-life of oil can be very short if milled or grated coconut kernel is not properly prepared prior to oil extraction. Oil drying is recommended to ensure long shelf-life. Produces low-fat, high-fibre coconut milk as a coproduct. Long shelf-life of oil – 1 year and above. Can also be used in tandem with desiccated coconut processing. High investment cost since it uses highly specialised equipment and is energy intensive. Very intense, fresh coconut aroma. FFA: 0.1% MC: 0.14% and below if heating is done long enough to remove water in the coconut milk Colour - water-clear to pale yellow depending on the heating process Very low investment cost. Can be produced on a home scale operation using ordinary kitchen utensils. Produces a wet coconut residue that has to be further dried or processed to have market value. Produces a by-product (proteinaceous residue) FFA: 0.1–0.2% MC: 0.17% and below Colour: water-clear Oil recovery: 25 kg per 100 kg of fresh grated coconut kernel (based on 50% initial MC of kernel) Fresh-wet processes 6. Modified kitchen method 29 Oil recovery - 16.5 kg per 100 kg of fresh grated coconut kernel (based on 50% initial MC of kernel) which does not have commercial value at present. Oil drying is recommended to prolong shelf-life. Hardest to control in getting the correct colour and low MC. 7. Modified natural fermentation method FFA: 0. 1% MC: 0.12% and below Colour - water-clear Oil recovery - 34 litres per 100 litres of coconut milk (about 19 kg oil per 100 kg of fresh grated kernel) (Based on 50% initial MC of kernel) Very low investment cost. Lowest labour and energy input. Can be produced quickly on a home scale operation using ordinary kitchen utensils or on small/medium scale operation using semimechanised equipment. Disposal of fermented skim milk could be a big problem if done on a medium scale plant operation. Oil produced has a faint sour smell which can be removed by ageing. Produces premium and class B grades of VCO. Uses a lot of potable water. 8. Fresh-wet centrifuge method (2phase centrifuge) FFA: 0.04–0.08% MC: 0.1% and below Colour: water-clear Oil recovery: about 28 litres oil per 100 litres of coconut milk (about 17 kg oil per 100 kg fresh grated kernel) (Based on 50% initial MC of kernel) Reported oil recovery rate was computed from the information provided Produces the best quality coconut oil with best sensory attributes if done in a two stage centrifuge process. Can only be applied in a medium scale operation as investment cost is very high. Optimisation of the process is still required to improve oil recovery rate. Current oil recovery rates are much lower than the modified fermentation 30 by a VCO producer using a 2-phase centrifuge. Oil recovery rate using a 3-phase centrifuge may be different. 9. Bawalan-Masa process 10. CFTRI process FFA - 0.05–0.08% MC - 0.07–0.12% Colour – water- clear VCO from kernel Oil recovery - 17 kg fibre residue left per 100 kg of wet after expelling residue Coconut coconut milk. flour - 26.3 kg per 100 kg of wet residue Enzyme assisted freshwet centrifuge method FFA- 0.12 % MC- 0.21% Colour – water- clear Oil recovery – Upto 27 kg per 100 kg of fresh grated coconut kernel (based on 50% initial MC of kernel) process. Lowest extraction efficiency. Further processing of the coconut skim milk into health beverage and the sapal generated into coconut flour can improve profitability Further recovery of high value oil from residue makes coconut milk/VCO processing more profitable. Long shelf-life of oil – 1 year and above. Produces low fat high fibre coconut flour as a byproduct. Requires mechanical type of equipment to produce the oil. Production process has to be attached or integrated to an existing coconut milk processing plant or a high capacity VCO plant. Maximises the income from coconut kernel when used in tandem with coconut milk processing or the fresh-wet centrifuge process of VCO production. Vitamin E content in oil is high (6.2 mg/100ml). Very good sensory attributes with intense, fresh coconut aroma. Source: Updated and revised table from Bawalan (2011) 31 Chapter 1C Biotechnological Processing for Value Added Products from Coconut Biotechnology has played a vital role in different spheres of life. It is the use of biological processes, organisms, or systems to manufacture products intended to improve the quality of human life. It has made a significant impact in health care (development of vaccines and other biologicals), agriculture (development of hybrid plants), etc. as well as in food processing sector. The advances in application of biotechnology in food processing mainly concerns with the approaches such as strain improvement of microorganisms for the production of fermented foods (like curd and soy sauce) and use of enzymes for the manufacture of processed foods (like cheese and juices). It also has helped in improving the edibility, texture and storage of the food. Various amino acids, food-flavouring agents, food additives and preservatives are derivatives obtained from processing of biomass of microbial, plant or animal origin. A bioprocess involves biomass pretreatment, fermentation or biocatalysis and finally downstream processing (Yang, 2011) or isolation from natural source by physical/chemical methods before subjecting to downstream processing. It finds its application in oilseed processing and refining, starch and protein processing, non-thermal food processing (eg. filtration), fermentation, extraction techniques, enzymatic conversions, and packaging. In the coconut industry, bioprocessing is inevitable as the different products obtained are a result of different unit operations such as drying, fermentation, extraction, etc. Bioprocess engineering is a specialized branch of biotechnology which involves design and development of equipment and processes for the production of value added products. This technology has revolutionised the scale at which coconut products can be manufactured 32 compared to traditional methods especially for oil, desiccated coconut and copra production. Fermentation is known to conserve properties of food for long term storage, biotransformation and improvement in assimilation of nutrients. Products such as coconut toddy, vinegar, Nata-de-coco and virgin coconut oil (fermentation method) involves use of microorganisms for bioconversion using coconut as raw material. Coconut pro-biotics can also be prepared from coconut water or coconut milk using Kefir or Kombucha (symbiosis of lactic acid bacteria and yeast) as starter culture (Tietze et al., 2006). With increasing demand for nondairy products and some noise on the World Wide Web about soy not being healthy, coconut yoghurt has also been launched in the market (Aoyagi, 2013). Enzymes are used in the food industry for achieving high product yields and avoiding energy intensive unit operations with severe operational conditions. Domínguez et al. (1994) reported that most extraction processes (mechanical pressing and solvent extraction) suffer from major drawbacks with regard to economic, environmental and safety aspects. The high temperatures and solvents used cause undesirable side effects on the quality of the finished product and the protein obtained is often denatured, thus limiting its use for food and feed products (Johnson and Lusas, 1983). These problems could be overcome by the aqueous processing of oil seeds. Simultaneous aqueous processing and enzymatic treatment can favour the extraction of oil in environmentally safe and economical manner, also yielding an edible protein product. Lower operation temperature can be used, with consequent lower energy requirements. In the case of coconut, aqueous enzymatic processing 33 is a possible alternative technology for the extraction of oil and the protein. A number of workers have reported utilization of enzymes for extraction of coconut oil. McGlone et al. (1986) reported an extraction method of coconut oil based on the action of different enzymes like polygalacturonases, αamylase and proteases on a diluted coconut paste. About 80% yields were obtained by this method with an added advantage of very low energy inputs. Barrios et al. (1990) studied the effect of pectinase, α-amylase, proteases, cellulases and - glucanases at different concentrations and temperatures on extraction of coconut oil. This study on a laboratory as well as pilot scale indicated higher yield of oil and considerably low capital investment but suffers from drawbacks such as high cost of enzymes, requirement of fresh water and treatment of wastewater prior to disposal. Rosenthal et al. (1996) and Ricochon and Muniglia (2010) have comprehensively reviewed aqueous and enzyme based processes and discussed related issues for edible oil extraction. All studies indicate that different enzymes are required to degrade different components of the structural cell wall (mannan, galactomannan and cellulose) thus releasing oil from cells (Figure 1C.1). Enzymes also decrease emulsion stability during coconut oil extraction, resulting in rapid oil separation. Raghavendra and Raghavarao (2010) reported the effect of different treatments like thermal, pH, chilling, enzyme treatments and combination of enzyme treatment followed by chilling, thawing for the destabilization of coconut milk emulsion to obtain virgin coconut oil. The highest yield of 94.5% was observed in the coconut milk sample treated with 0.1% v/v of protease at 37°C followed by chilling and thawing to ambient conditions. Proteins (which act as emulsifiers in coconut milk) are broken 34 down into smaller peptides by protease, decreasing their emulsifying ability. This leads to aggregation of oil droplets, destabilization of the emulsion and results in higher oil yields (Figure 1C.2). 35 Figure 1C.1: Effect of enzymatic treatment on oil seed cell structure Source: Rosenthal et al. (1996) 36 Figure 1C.2: Effect of enzymatic treatment on coconut milk emulsion 37 Chapter 1D Aim and Scope of Present Work In order to fullfill the objectives, methods for dehydration of fresh coconut and copra gratings, an improved process for production of virgin coconut oil and methods for value addition to the byproducts were developed. This thesis consists of five chapters, of which chapter 1 contains general introduction about coconut, coconut products and processing as well as biotechnological processing for value added products from coconut. Chapter 2 deals with process for production of coconut oil from fresh coconut and copra gratings. The choice of dryer to dehydrate coconut gratings based on product quality was made. Drying kinetics of coconut gratings was studied. Effect of pretreatment of copra with enzymes on expelling of oil copra was investigated. Coconut milk is an oil-in-water emulsion that is expelled from fresh coconut. In chapter 3, the first section consists of stabilization studies of coconut milk using silica nanoparticles and comparison with other commercially used stabilisers. In the second section, effect of different treatments (emzymatic, pH and ultrasound) for destabilization of coconut milk for the production of VCO was studied. An improved process for production of VCO was also developed. In chapter 4, value addition to coconut skim milk was attempted by dehydration (drum drying, spray drying and freeze drying) and concentration (ultrafiltration) in two sections. The effect of dehydration methods was studied on functional properties and sensory quality of product. The process parameters considered in ultrafiltration (UF) were standardized with respect to transmembrane flux, protein retention and sugar removal. 38 Chapter 5 deals with production, characterization and storage studies of coconut protein powder (CPP). Functional properties of CPP were evaluated and compared to defatted soybean powder and cow skimmed milk powder. Storage studies of CPP were carried out in order to estimate the shelf life of the product. Sensory and instrumental analysis of CPP was carried out. A pilot consumer acceptance test was carried out to rate the acceptability of product. 39
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