Research Article
141
Integral membrane proteins Brr6 and Apq12 link
assembly of the nuclear pore complex to lipid
homeostasis in the endoplasmic reticulum
Christine A. Hodge1,*, Vineet Choudhary2,*, Michael J. Wolyniak1,*,‡, John J. Scarcelli1,§, Roger Schneiter2,¶
and Charles N. Cole1,3,¶
1
Department of Biochemistry and 3Department of Genetics, Dartmouth Medical School, Hanover, NH 03755, USA and 2Division of Biochemistry,
Department of Medicine, University of Fribourg, CH-1700 Fribourg, Switzerland
*These authors contributed equally to this work
‡
Present address: Department of Biology, Hampden-Sydney College, Hampden-Sydney, VA 23943, USA
§
Present address: Tetragenetics, Inc., One Broadway, 14th Floor, Cambridge, MA 02142, USA
¶
Authors for correspondence ([email protected]; [email protected])
Journal of Cell Science
Accepted 20 October 2009
Journal of Cell Science 123, 141-151 Published by The Company of Biologists 2010
doi:10.1242/jcs.055046
Summary
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Key words: Membrane dynamics, Nucleocytoplasmic transport, Lipid metabolism, Nucleoporins, Nuclear envelope
Introduction
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Journal of Cell Science
142
Journal of Cell Science 123 (1)
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Results
Genetic interactions between BRR6 and APQ12
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NPC biogenesis and lipid homeostasis
Journal of Cell Science
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brr6-1 affects the assembly of NPC filaments
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Journal of Cell Science
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Overexpression of Brr6 restores normal episterol levels to
apq12! cells
Journal of Cell Science
Production of neutral lipids is essential for viability of brr6-1
NPC biogenesis and lipid homeostasis
Journal of Cell Science
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148
Journal of Cell Science 123 (1)
Discussion
Journal of Cell Science
Genetic interactions and similar mutant phenotypes for
brr6-1 and apq12
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Journal of Cell Science
Lipid esterification is essential for viability of brr6-1
mutant cells
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Materials and Methods
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150
Journal of Cell Science 123 (1)
A*..*3,"7#'%+*-.'>,"7H#@.$,-#'-,5#'"5#1'.6,+,-#'-,5#3$($#'55$5#2$A*($#'%+*-.'>,"7
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+4$/$#'7$"+/0#/+(',"/#3$($#5,.%+$5#+$"A*.5#A*%(#+,6$/#'-(*//#(*3/#*A#'#_FP3$..#1.'+$0
'"5#\#.#/'61.$/#A(*6#+4$#*(,7,"'.#'"5#5,.%+$5#/'61.$/#3$($#/1*++$5#*"+*#g;S#1.'+$/
/%11.$6$"+$5#3,+4#+4$#'11(*1(,'+$#'7$"+H#;.'+$/#3$($#7(*3"#A*(#c#5')/#'+#DKZ<0#KEZ<
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'A+$(#X#'"5#Y\#5')/H
Journal of Cell Science
Lipid analysis
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Supplementary material available online at
http://jcs.biologists.org/cgi/content/full/123/1/141/DC1
References
8+>"4% C$4% A<*.R4% C$% S$4% T"U*?.*#3*F*/4% V$% */:% 8>+<)(/4% W$ 9Y__E=H# Q# )$'/+# 6%+'"+0
;L;DE0# '.+$($5# ,"# 6L:Q# 6$+'2*.,/6# '"5# 6',"+$"'"-$# *A# +4$# "%-.$'(# /+(%-+%($0# ,/
5$A$-+,>$# ,"# '# 7$"$# 4*6*.*7*%/# +*# +4$# 4%6'"# 7$"$# L<<Y# 34,-4# ,/# ,">*.>$5# ,"# +4$
-*"+(*.#*A#-4(*6*/*6$#-*"5$"/'+,*"H#R&FM+N)GM+N)G).M BBI0#XDPIEH
8/2(/"/4%S$4%E<<+/>+.#4%W$%*/:%',<2=4%E$ 9DEEI=H#:%-.$'(#1*($#-*61.$8#'//$62.)#+4(*%74
+4$#-$..#-)-.$O#($7%.'+,*"#'"5#6$62('"$#*(7'",N'+,*"H#VB3!+L)..M KPB0#DEE\PDEYFH
@,22R+4%J$%C$4%W(/+)4%X$%'$%*/:%@<(;34%Y$ 9Y_IE=H#JAA$-+#*A#/+$(*.#/,5$#-4',"/#*"#7(*3+4
'"5#6$62('"$#A'++)#'-,5#-*61*/,+,*"#*A#!"##$"%&'(#)*+#)%),-*-")H#WM+3"#.)%-&FM &II0
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