®
easySpiral Dilute
The world's first automatic diluter & plater
A revolution
in serial dilutions!
Our quality for your lab
• Designer and manufacturer for microbiology
• R&D leadership for innovative and reliable products
• Worldwide distribution network in more than 80 countries
interscience
R&D center and manufacturing plant
Mourjou FRANCE
• Made in France
®
Spiral method:
35 years experience
The Spiral® method was designed in 1973 to automate the routine
work of bacterial enumeration by Dr. Ed Campbell, researcher at the
FDA (Food & Drug Administration). With François Jalenques, friend
and founder of interscience, they patented an updated method
in 1992.
Since then Spiral® automatic platers have been a reference for
applications in food microbiology, medical bacteriology, research on
food preservatives or cosmetological factors in compliance with the
AFNOR V08-100 and ISO 7218 standards.
Today interscience is proud to launch the 4th generation of
Spiral® platers with easySpiral® Dilute: the world's first automatic
diluter & plater.
F. Jalenques & Dr. Ed Campbell
1977
Spiral® DS
First Spiral® plater
1996
Spiral® DS+
Automatic Spiral® plater
2010
easySpiral®
World's fastest Spiral® plater
PATENTED
2012
easySpiral® Dilute
World's first automatic diluter & plater
PATENTED
interscience is proud to introduce easySpiral® Dilute, a 2-in-1 automatic diluter and plater, which allows you
to do 5 x 1/10th serial dilutions. It will then automatically plate on 1 single Petri dish, with a countable range of 30 to
10 million countable CFU/mL.
2
Plate
Place sample
1
Dilute
Place your sample in the beaker
1. The easySpiral® Dilute will automatically dilute the sample
2. The easySpiral® Dilute will automatically plate the sample on a Petri dish
Why use an automatic diluter & plater ?
easySpiral® Dilute allows you to save 50 % on consumables and 50% on your time.
Comparison between manual and automatic method below.
Example of 50 samples: Dilution: 10-7
Plating: 10-5, 10-6 and 10-7
Manual method
Automatic method
CONSUMABLES
CONSUMABLES
Dilution
Dilution
• 400 tubes
• 350 beakers
• 350 pipet tips
Plating
Plating
• 450 Petri dishes
• 150 Petri dishes
TIME
10h10
TIME
5h50
3
Key features
Compact
High precision glass syringe
38 cm wide, can be used inside and outside a laminary flow
Stainless steel housing
Typical accuracy of 0.5 %
No more pipet calibration
Fast rotating arm
5 dilutions and 1 plating in 100 sec.
PATENTED
Automatic disinfection
One button touch
Using Overflow technology
No cross contamination
For 10-5 dilution and plating
PATENTED
PATENTED
4
Direct access
Autoclavable diluent
and disinfectant bottles
Pre-set dilutions and plating
No more 9 mL tube preparation
How does it work?
Dilution
1
Sample intake
sample
2
0.5 mL of sample is deposited
sample
10-1
sample
10-1
3
4.5 mL of diluent is added to the sample and mixed
The above dilution cycle is repeated for the next serial dilution.
0.5 mL of 10-1 diluted sample intake
1
sample
10-1
sample
10-1
2
0.5 mL of 10-1 dilution is deposited
10-2
Dilution cycle can be repeated as many times as you wish.
Plating
Constant mode
Spiral® mode
Circle mode
replicates the manual plating
with a homogenous deposition.
allows a decreasing
concentration deposition.
allows the plating
of 3 dilutions on 1 Petri dish.
50 µL, 100 µL, 200 µL
and USB programmable
50 µL, 100 µL, 200 µL
and USB programmable
10 µL inner circle, 27 µL middle circle
and 56 µL outer circle
Examples:
Before
incubation
Homogenous deposition
Concentrated
Less concentrated
10-1 10-2 10-3
After
incubation
How to count?
Manual colony counting
Automatic colony counting
Scan® 100
or
with Spiral®
counting grid
Scan® 300
Scan® 500
Scan® 1200
5
Questions
What is special about
the easySpiral® Dilute ?
easySpiral® Dilute is the only 2-in-1 equipment
on the market making automatic serial dilutions and standardized plating.
I usually use 9 mL tubes
and 1 mL sample...
The accuracy of
easySpiral® Dilute plater
is high: 0.5%.
How can I check it?
It is easy and automatic.
Press on the test procedure button which fills
a beaker with 0.5 mL and another one with
4.5 mL. Weight the dispensed volume to verify the results.
The easySpiral® Dilute has a 0.5 % precision
and typical accuracy on 4.5 mL and 0.5
mL volumes thanks to its high precision
HamiltonTM glass syringe.
Common precision for the filling of the 9 mL
tubes is 2% and pipettes precision for the
1 mL intake is 2%, and they must be often
calibrated and checked.
Conclusion, you can obtain much higher
precision with volumes cut by half!
I need to make 10-7 dilutions.
How can I do it ?
Can I use peptone water
as diluent?
No, because all diluents with nutriments
can result in contamination of the machine
and of the sample.
You can use any saline solution, for example
ringer solution or NaCl solution. As the
bacteria stay in the beakers for only a few
seconds before going on a nutriment solution
on the agar, the dilution with only a saline solution has no consequence on the overall count.
6
The easySpiral® Dilute can do as many
dilution as you wish.
Do a 10-5 dilution, take the last beaker and
place it as the mother sample and then
proceed with a new automatic dilution
sequence.
Accessories & technical specifications
Housing protection
Beakers DB 50
Barcode reader
for working outside laminar flow
sterile beakers
barcode reader
Bottle set
connection GL 45
for GL 45 bottles
Ref 413 001
Ref 415 100
Ref 413 003
Ref 522 000
easySpiral® Dilute is delivered with 1 000 sterile beakers, 1 blue dye for tests, 1 detergent liquid, 3 sets of GL 45 bottle connectors, 55, 90 mm & 150 mm Petri dish ring,
Spiral® counting grids: 90 mm & 150 mm, test container stand, power cord, user’s manual, , monitoring CD-ROM software, USB cable.
Reference
Petri dish diameter
Syringe capacity
Dispensed volume
Preset volume dispensed (programmable)
Detection in CFU/mL
Plating modes
Number of dilutions at 1/10th for 1 cycle
Full dilution cycle
Full plating cycle
Full cycle (disinfection, 5 dilutions, plating of 6 dishes)
Diluent autonomy
Patented circle mode
Stylus disinfection system
Overflow technology (patented)
Monthly cleaning program
Cleaning capacity
Successive plating capacity with the same sample
Traceability
Programmable volume via USB
Filling time programmable via USB (for viscous samples)
Sample mixing before dilution/plating
Controlled by microprocessor
Dimensions
Weight
Power supply
1 year guarantee (after registration)
All stainless steel
Made in France
easySpiral® Dilute
414 000
55 mm, 90 mm and 150 mm
1 000 µL
from 10 to 1 000 µL
50, 100 and 200 µL
30 to 10 million CFU/mL
exponential, uniform and circles
5
100 sec.
25 sec.
3 min. 40 sec.
55 cycles of 5 dilutions (with 2L bottles)
3 dilutions on one 90 mm Petri dish
6 dilutions on one 150 mm Petri dish
inside and outside by Overflow
8 bars pressure
D
600 cycles (with 2L bottles)
10 Petri dishes (50 µL)
Excel™ export
D
D
D
D
39 x 42 x 29 cm
15.6 kg
100-240 V~ / 50 Hz to 60 Hz
D
D
D
WEEE
RoHS
2002/96/EC
2002/95/EC
ISO
9001
2008
MADE IN
FRANCE
7
USA & CANADA
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[email protected] - www.intersciencelab.com
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Distributor
www.
interscience .fr
easySpiral Dilute_B_0612. Pictures and information in this brochure are not contractually binding. easySpiral® is registered trademark. INTERSCIENCE reserves the right to change or improve the specifications of its products without notice. Please visit www.interscience.fr for current updates and additional information.
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