Org. Divers. Evol. 3, Electr. Suppl. 10, part 1: 1 - 4 (2003)
© Gesellschaft für Biologische Systematik
http://senckenberg.de/odes/03-10.htm
Part 1.
Multiple amino acid sequence alignment of 35 β-tubulin proteins.
Electr. Suppl. 10, part 1. − to:
Einax, E., Voigt, K. (2003):
Oligonucleotide primers for the universal amplification of β-tubulin genes facilitate phylogenetic
analyses in the regnum Fungi.
Org. Divers. Evol. 3(3): 185-194.
Multiple amino acid sequence alignment of 35 β-tubulin proteins (see Table 1 in the printed paper) representing
the N- and C-termini (A) and the middle part (B). The alignment is partial and shows four well-conserved regions
which are suitable priming sites. The complete amino acid sequences of 18 organisms were used to determine the
N-terminal βtub1 and the C- terminal βtub4r region (A), whereas all 35 peptides could be used to define the βtub3
and βtub2r sites in the middle part of the β-tubulin protein (B). Gaps in the alignment are indicated by hyphens (-).
Perfectly conserved positions are labelled with asterisks (*), whereas dots (.) mark less conserved amino acid
positions. Unmarked regions imply sites with variable amino acid sequences. All sequences are available from
GenBank (http://www.ncbi.nlm.nih.gov).
A (N- and C-termini)
Bombyx mori
Octopus dofleini
Brugia pahangi
Epichloe typhina
Neurospora crassa
Aspergillus flavus
Erysiphe graminis
Schizophyllum commune
Coprinus cinereus
Pleurotus sajor-caju
Uromyces fabae
Melampsora lini
Phytophthora cinnamomi
Pythium ultimum
Achlya klebsiana
Oryza sativa
Zea mays
Schizosaccharomyces pombe
MREIVHVQVGRCGNQIGSKFWEVISDEHGIDPCGRYHGDSDLQLERINVY
MREIVHIQAGQCGNQIGSKFWEVISEEHGIDPSGVYQGKLSTQLERSYVY
MREIVHVQAGQCGNQIGAKFWEVISDEHGVQPDGTYKGDSDLQIERINVY
MREIVHLQTGQCGNQIGAAFWQTISGEHGLDSNGVYNGTSELQLERMSVY
MREIVHLQTGQCGNQIGAAFWQTISGEHGLDASGVYNGTSELQLERMNVY
MREIVHLQTGQCGNQIGAAFWQTISGEHGLDGSGVYNGSSDLQLERMNVY
MREIVHLQTGQCGNQIGAAFWQTISGEHGLDGSGVYNGTSDLQLERMNVY
MREIVHLQTGQCGNQIGAKFWEVVSDEHGIEADGLYKGTNDQQLERISVY
MREIVHLQTGQCGNQIGAKFWEVVSDEHGIERDGLYKGNNDLQLERISVY
MREIVHLQTGQCGNQIGAKFWEVVSDEHGIEPDGLYKGNNDLQLERISVY
MREIVHLQTGQCGNQIGAKFWEVVSDEHGIATDGQYKGNTDLQLERISVY
MREIVHLQTGQCGNQIGAKFWEVVSDEHGIATDGQYKGTSDLQLERISVY
MRELVHIQGGQCGNQIGAKFWEVISDEHGVDPTGSYHGDSDLQLERINVY
MRELVHIQGGQCGNQIGAKFWEVISDEHGVDPTGSYHGDSDLQLERINVY
MRELVHIQGGQCGNQIGAKFWEVISDEHGVDPTGSYHGDSDLQLERINVY
MREILHIQGGQCGNQIGAKFWEVICDEHGIDHTGKYSGDSDLQLERINVY
MREILHIQGGQCGNQIGAKFWEVVCAEHGIDATGRYGGDSDLQLERVNVY
MREIVLIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVY
***.. .* *.****.*. ** .. .***.. * * *. . * ** **
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
βtub1
Org. Divers. Evol. 3, Electr. Suppl. 10, part 1 (2003)
Einax & Voigt: Primers for β-tubulin gene analyses in fungi
Bombyx mori
Octopus dofleini
Brugia pahangi
Epichloe typhina
Neurospora crassa
Aspergillus flavus
Erysiphe graminis
Schizophyllum commune
Coprinus cinereus
Pleurotus sajor-caju
Uromyces fabae
Melampsora lini
Phytophthora cinnamomi
Pythium ultimum
Achlya klebsiana
Oryza sativa
Zea mays
Schizosaccharomyces pombe
2
EGMEEGDFAEADNNVSDLLSEYQQYQDATIDQEFEDEEEVEEQNDDSDEQ
EGMDEMEFTEAESNMNDLVSEYQQYQEARSTDS-D-EYDNEEY-YNQQEE
EGMDEMEFTEAESNMNDLVSEYQQYQDATADEE-G-DLQEGESEYIEQEE
EGMDEMEFTEAESNMNDLVSEYQQYQDAGIDEE-EEEYEE-EAPVDEPLE
EGMDEMEFTEAESNMNDLVSEYQQYQDAGVDEE-EEEYEE-EAPLEGE-E
EGMDEMEFTEAESNMNDLVSEYQQYQDASISEG-EEEYLE-EEEPLEHEE
EGMDEMEFTEAESNMNDLVSEYQQYQEASISEG-EEEY---PEEVSNEEE
EGMDEMEFTEAESNMQDLVAEYQQYQDATV-EE-EGE---YEEEVIEDQE
EGMDEMEFTEAESNMQDLVAEYQQYQDATV-EE-EGE---YEDEVLEDEQ
EGMDEMEFTEAESNMQDLVAEYQQYQDATADEE-EGE---YEEEPAEEEQ
EGMDEMEFTEAESNMQDLVAEYQQYQEAHMDDE-EA-EEAYEDEAPPEEEGMDEMEFTEAESNMQDLVAEYQQYQEAHVDED-EVDEEVYEDEAPPEEEGMDEMEFTEAESNMNDLVSEYQQYQDGTAE-E-EGEFD--EDEEW--MR
EGMDEMEFTEAESNMNDLVSEYQQYQDATAE-E-EGEFD--EDEEMDEMM
EGMDEMEFTEAESNMNDLVSEYQQYQDATAE-E-EGEFD--EDEEMDEMM
EGMDEMEFTEAESNMNDLVAEYQQYQDATADDE-EEDYG--DEEEDEVAA
EGMDEMEFTEAESNMNDLVSEYQQYQDATAD-E-EGEYE--DEEEGDLQD
EGMDEMEFTEAESNMNDLVSEYQQYQEAGIDEG-DEDYEI-EEEKEPLDY
***.* .*.**..*. **..******..
. .
450
447
448
448
447
448
446
445
445
446
447
448
444
446
444
447
446
448
β tub4r
Org. Divers. Evol. 3, Electr. Suppl. 10, part 1 (2003)
Einax & Voigt: Primers for β-tubulin gene analyses in fungi
3
B (middle part)
Bombyx mori
Octopus dofleini
Brugia pahangi
Epichloe typhina
Neurospora crassa
Aspergillus flavus
Erysiphe graminis
Schizophyllum commune
Coprinus cinereus
Pleurotus sajor-caju
Uromyces fabae
Melampsora lini
Phytophthora cinnamomi
Pythium ultimum
Achlya klebsiana
Oryza sativa
Zea mays
Schizosaccharomyces pombe
Syncephalis depressa
Spiromyces minutus (btub2)
Basidiobolus ranarum (btub1)
Basidiobolus ranarum (btub2)
Rhizophlyctis rosea
Harpochytrium sp. 94 (btub1)
Spizellomyces punctatus (btub1)
Conidiobolus coronatus (btub1)
Conidiobolus coronatus (btub2)
Entomophaga maimaiga
Spiromyces minutus (btub1)
Rhizopus microsporus (btub1)
Rhizopus microsporus (btub2)
Rhizopus microsporus (btub3)
Microsporus ramannianus
Spizellomyces punctatus (btub2)
Linderina pennispora
WAKGHYTEGADLLETVLDVVRKEAEGCDCLQGFQLVHSLGGGTGSGMGTL
WAKGHYTEGAELVDTVLDVIRKECEGCECIQGFQMTHSLGGGTGAGMGTL
WAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTL
WAKGHYTEGAELVDQVLDVVRREAEGCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDQVLDVVRREAEGCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDQVVDVVRREAEGCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDQVLDVVRREAEGCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDAVLDVVRKEAEGTDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDAVLDVVRKEAEGTDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELIDSVLDVVRKEAESCDCLQGFQITHSLGGGTGSGMGTL
WAKGHYTEGAELIDSVLDVARKEAESCDCLQGFQITHSLGGGTGSGMGTL
WAKGHYTEGAELIDSVLDVVRKEAESCDCLQGFQITHSLGGGTGSGMGTL
WAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTL
SAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSAMGTL
WAKGHYTEGAELAVAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTL
WAKGHYTEGAELVDQVLDIARKEAEGCDSLQGFQFTHSLGGGTGSGMGTL
WAKGHYTEGAELVDSVLDVVRKEVENCDCLQGFQITHSLGGGTGSGMGTL
WAKGHYTEGAELVDSVLDVVRKEAEGCDGLQGFQITHSLGGGTGSGLGTL
WAKGHYTEGAELVDSVLDVVRKEAEGCDSLQGFQITHSLGGGTGSGMGTL
WAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDSVLDVVRKEAENCDCLQGFQITHSLGGGTGAGMGTL
WAKGHYTEGAELVDSVLDVVRKEAENCDCLQGFQITHSLGGGTGAGMGTL
WAKGYYTEGAELVDNILEIVRKETENCDSLQGFQVAHSLGGGTGSGLGSL
WAKGHYTEGAELVDSVLDVVRKEAENSDSLQGFQITHSLGGGTGSGMGTL
WARGYYTEGAELVESVMDVLRKEAENTDCLQGFQLCHSLGGGTGSGLGSL
WAKGYYTEGAELVESIMDVVRKEAENTDCLQGFQLAHSLGGGTGSGLGSL
WAKGYYTEGAELVESIMDVVRKEAENTDCLQGFQLAHSLGGGTGSGLGSL
WAKGYYTEGAELVESILDVVRKEAEHTDCLQGFQLCHSLGGGTGSGLGSL
WAKGYYTEGAELVESVLDIVRKESEGCDSLQGFQMVHSLGGGTGSGLGSL
WAKGFYTEGAELLDQVLDTIRQDVERCDLLSGFQLCHSIAGGTGSGMGSL
*.* *****.*
* . * . ..*** **..****...*.*
150
150
150
150
150
150
150
150
150
150
150
150
150
150
149
150
150
150
135
135
135
135
135
135
127
135
135
135
135
135
135
135
135
127
135
β tub3
Org. Divers. Evol. 3, Electr. Suppl. 10, part 1 (2003)
Einax & Voigt: Primers for β-tubulin gene analyses in fungi
Bombyx mori
Octopus dofleini
Brugia pahangi
Epichloe typhina
Neurospora crassa
Aspergillus flavus
Erysiphe graminis
Schizophyllum commune
Coprinus cinereus
Pleurotus sajor-caju
Uromyces fabae
Melampsora lini
Phytophthora cinnamomi
Pythium ultimum
Achlya klebsiana
Oryza sativa
Zea mays
Schizosaccharomyces pombe
Syncephalis depressa
Spiromyces minutus (btub2)
Basidiobolus ranarum (btub1)
Basidiobolus ranarum (btub2)
Rhizophlyctis rosea
Harpochytrium sp. 94 (btub1)
Spizellomyces punctatus (btub1)
Conidiobolus coronatus (btub1)
Conidiobolus coronatus (btub2)
Entomophaga maimaiga
Spiromyces minutus (btub1)
Rhizopus microsporus (btub1)
Rhizopus microsporus (btub2)
Rhizopus microsporus (btub3)
Microsporus ramannianus
Spizellomyces punctatus (btub2)
Linderina pennispora
4
AACDPHRGRYLTVATVFRGRMSMKEIDEQILNVQKKNKDFFVEWIPNNVQ
TACDPRHGRYLTVAAVFRGKMSMKEVDEQMFSIQNKMSPYFVEWIPNNVK
AACDPRHGRYLTVAAMFRGRMSMREVDEQMMQVQNKNSSYFVEWIPNNVK
AASDFRNGRYLTCSAIFRGKVAMKEVEDQMRNVQNKNSSYFVEWIPNNIQ
AASDFRNGRYLTCSAIFRGKVSMKEVEDQMRNVQNKNSSYFVEWIPNNVQ
AASDFRNGRYLTCSAIFRGKVSMKEVEDQMRNIQSKNQTYFVEWIPNNIQ
AASDFRNGRYLTCSAIFRGKVSMKEVEDQMRNVQQKNVSYFVEWIPNNVQ
AASDPRHGRYLTVAAMFRGKVSMKEVEEQMQNVQNKNSAYFVEWIPNNVL
AASDPRHGRYLTVAAVFRGKVSMKEVEEQMQNVQNKNSAYFVEWIPNNVL
AASDPRHGRYLTVAAVFRGKVSMKEVEEQMQNVQNKNSAYFVEWIPNNVL
AASDPRHGRYLTVAAYFRGKVSMKEVEENMLSVQNKNSNYFVEWIPNNVQ
AASDPRHGRYLTVAAYFRGKVSMKEVEENMLSVQSKNSNYFVEWIPNNVQ
CAADPRHGRYLTAACMFRGRMSTKEVDEQMLNVQNKNSSYFVEWIPNNIK
CAADPRHGRYLTAACMFRGRMSTKEVDEQMLNVQNKNSSYFVEWIPNNIK
CAADPRHGRYLTAACMFRGRMSTKEVDEQMLNVQNKNSSYFVEWIPNNIK
CAADPRHGRYLTASAMFRGKMSTKEVDEQMLNVQNKNSSYFVEWIPNNVK
CAADPRHGRYLTASAMFRGKMSTKEVDEQMLNVQNKNSSYFVEWIPNNVK
VAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVL
AASDPRHGRYLAVAAIFRGRISMKEVDEQMLLIQNKNSSYFVEWIPNNVK
AASDPRHGRYLTVAAMFRGRMSMREVDDQMINVQNKNSSYFVEWIPNNIK
AASDPRHGRYLTVAAMFRGRMSTKEVENQMINIQNKNSSYFVEWIPNNIK
AASDPRHGRYLTVAAMFRGRMSTKEVENQMINVQNKNSSYFVEWIPNNIK
AASDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
AASDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
AASDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
AASDPRHGRYLTVSAMFRGRLSMKEIDDQMLAIQNKNSAYFVEWIPNAVK
AASDPRHGRYLTVSAMFRGRLSMKEIDDQMLAIQNKNSAYFVEWIPNAVK
AASDPRHGRYLTVAAFFRGRLSMKEVEQQMVDVHKKNSNYFVEWIPHNIK
AASDPRHGRYLAVAAIYRGKVSTNEIDKTMLNVQNKNSSYFVEWIPNNVK
AASDPRHGRYLTVATIFRGRLSTKEVENQMLAVQQKNSSYFVEWIPNGVK
AASDPRHGRYLTAGTIFRGRLSTKEVENQMLAVQQKNSSYFVEWIPNSVK
AASDPRHGRYLTAGTIFRGRLSTKEVENQMLAVQQKNSSYFVEWIPNSVK
AASDPRHGRYLTVATIFRGRLSTKEVENQMLAVQQKNSSYFVEWIPNSVK
AACDPRHGRYLTVSTIFRGKVSTKEVEQQMYNMQSRNSSYFVEWIPNNIK
VDVDPRHGRYLTCAAMFRGKVSVKECEDTIWNYAEKNASSFVEWIPNSTQ
. * ..****. . .**... * .. .
.
******...
350
350
350
350
350
350
350
350
350
350
350
350
350
350
348
350
350
350
335
335
335
335
335
335
327
335
335
335
335
335
335
335
335
327
β tub2r
Org. Divers. Evol. 3, Electr. Suppl. 10, part 1 (2003)