Tobacco Education: KS3 Science Experiments and Activities 1. Nicotine Simulated Blood Vessel Restriction/Heart Rate Increases Simulated Blood Vessel Restriction 2. Tobacco – Tar Tar accumulation Tar accumulation over a One-Year Period Tar’s paralyzing Effects on Bronchial Cilia Obstructive properties of Tar Effects of Tar on plants 3. Carbon Monoxide and Other Poisons Oxygen/Carbon Monoxide Simulation Poisons in Tobacco Smoke How Cigarette Smoke Affects Mould Growth on Bread 4. Lung capacity 5. Smokeless Tobacco Tobacco juice Tobacco juice stains The purpose is to demonstrate the effects of the poisons in tobacco, focusing primarily on nicotine, Tar, and carbon monoxide. 1. Nicotine Nicotine is a poisonous, water-soluble alkaloid found in tobacco leaves and used as an insecticide. One cigarette contains approximately 8 milligrams of nicotine. Nicotine increases heart rate, and blood pressure rises when the blood vessels constrict. Nicotine affects the body in only three-and-a-half seconds, and the effect lasts approximately 90 minutes. Simulated Blood Vessel Restriction/Heart Rate Increases Purpose -- To simulate the effect of nicotine on the blood vessels. Equipment � Small C-clamp � Bicycle pump Procedure One child pumps the bicycle pump. Another child tightens the C-clamp on the pump hose. As the clamp tightens, it will get harder to pump. A third child continually tells the first child to “pump faster.” Key Points Nicotine makes the heart beat faster and blood vessels smaller, which causes blood pressure to rise. NC Ks3 Science 1.1b, 1.2a, 1.4, 2.1, 3.3c Simulated Blood Vessel Restriction Purpose -- To simulate the effect of nicotine on the blood vessels. Equipment � 6 or more plastic or Styrofoam cups � 3 or more large diameter straws � 3 or more very small diameter straws (such as plastic coffee stirrers) � Water � Stopwatch Procedure � Fill all six glasses with equal amounts of water. Put the large diameter straws in three of the glasses. Put three very small diameter straws in the other three glasses. � On the word “GO,” have six children begin to drink as fast as they can (as if it were a race). � Time them using the stopwatch. Key Points � It should take much longer to drink the water using small straws. � Nicotine constricts the blood vessels which, in turn, decreases the blood flow and makes the heart have to work harder. NC Ks3 Science Science 1.1b, 1.2a, 1.4, 2.1, 3.3c 2. Tobacco – Tar Tar is a sticky substance found on tobacco leaves. Tar coats the lungs and air sacs in smokers, preventing them from getting enough oxygen. Tar paralyzes the cilia in a smoker’s windpipe so that dust particles and pollen are not swept out of the air passages. Tar contains more than 40 cancer-causing chemicals. Organs affected by these poisons include not only the mouth, vocal chords, throat, and lungs, but also the kidneys, bladder, and uterus and ovaries (in women). Tar Accumulation Purpose -- To demonstrate the amount of tar that can accumulate from one or more cigarettes. Equipment � Filterless cigarettes and matches � Cotton balls � “Smoking Machine”: A Turkey baster or empty ketchup bottle or Large syringe � Smoke cabinet to capture fumes. Procedure � Stuff cotton balls into the end of the smoking machine. � Tape an unfiltered cigarette onto the end of the smoking machine. � Light the cigarette and draw the smoke back through the cotton balls by pulling back on the syringe or by squeezing the bottle or baster. This could be done inside a smoke cabinet as with other gas experiments in science sessions. � Examine the tar accumulation by looking, smelling, and touching. � Video clip shows how 7.2g of tar accumulates in your lungs after smoking 400 cigarettes http://www.metacafe.com/watch/914475/still_smoking_watch_this/ Key Points � Not all of the tar was caught. � Smell the inside of the “smoking machine” to see what a smoker’s breath smells like. � The accumulation was from only one or two cigarettes. Imagine what the tar accumulation would be from a whole pack of cigarettes. Teaching Point: Can use the resource “Evidence bag” tar from a single cigarette here. NC Ks3 Science Science 1.1b, 1.2a, 1.4, 3.3c Tar Accumulation Over a One-Year Period Use the “Jar of tar” Eight ounces of tar can accumulate in the lungs of a person who smokes one pack of cigarettes per day for one year. NC Ks3 Science Science 1.1b, 1.2a, 1.4, 3.3c Tar’s Paralyzing Effects on Bronchial Cilia Purpose -- To graphically show how tar affects bronchial cilia. Equipment � Balls of various sizes � Use phlegm jar Procedure � Have children form two rows facing each other, with hands stretched out and barely touching each others’ finger tips. Fingers should be wiggling gently and slowly like cilia. � One end of the line represents the lungs; the other end represents the mouth. � The teacher places a ball in the hands of children at the “lungs” end of the line. The ball is slowly passed through the “mouth.” � Do the procedure while saying that the balls represent dust, dirt, pollen, and airborne particles that healthy cilia are able to carry out of the airways. � Then tell children that the cilia have been exposed to tobacco smoke and are now paralyzed and covered with sticky tobacco tar. � The teacher again places the balls in the hands of the children at the “lungs” end of the line, but the balls stay there because the cilia are paralyzed (do not move) and sticky. Key Points � Children who are around smokers have a higher rate of respiratory problems because dust and allergic particles stay in the lungs due to cilia paralyzed by tar accumulation. Adult smokers must cough forcefully to get the dust and dirt out of their lungs. Use phlegm jar to show what smokers cough up to clear lungs NC Ks3 Science Science 1.1b, 1.2a, 1.4, 3.3c Obstructive Properties of Tar Purpose -- To demonstrate how an accumulation of tar can inhibit the absorption of oxygen. Equipment � 2 coffee filters � Water � Funnel � Beaker or jar � tar-like substance (such as treacle) Procedure � Place the funnel in the jar. Line the funnel with filter paper, and pour in the water. Note how quickly it flows through. Remove the wet paper. � Coat another piece of filter paper with tar-like substance (treacle) and place it in the funnel. Pour water into the filter, noting how slowly the water flows through the coated paper. Key Points � The filter paper represents lung tissue. � The water represents oxygen. � The tar represents tobacco tar. � The lungs coated with tar do not exchange oxygen as well as healthy lungs without tar. NC Ks3 Science Science 1.1b, 1.2a, 1.4, 2.1 a, c, 3.3 c Effects of Tar on Plants Purpose -- To demonstrate the harmful effects of tar. Equipment � Smoking machine described in Tar Accumulation activity � 5 – 6 cigarettes and matches � Smoke cabinet � Cotton balls � Several plants � Latex gloves Procedure � Using the “smoking machine” inside the smoke cabinet, collect the tar accumulated from four to five cigarettes. � Wearing latex gloves, wipe the tar on the leaves and stems of several plants. Put a few plants aside to act as controls. � Record changes taking place with plants over a period of time. Plants should still be watered and have a source of sunlight or UV light. Period of time can ne in days and weeks. Key Points � What happened to the plants? Is one healthy and the other not? � What parts of the human body would tar touch? � What are some diseases humans could get from tar? NC Ks3 Science Science 1.1b, 1.2a, 1.4, 2.1, 3.3c 3. Carbon Monoxide and Other Poisons Carbon monoxide (CO) is an odourless, colourless gas that reduces oxygen levels in the blood. CO lessens night vision and hearing and is the same deadly gas found in car exhausts. Tobacco smoke is full of dozens of other poisons, such as arsenic, cyanide and lead. Oxygen/Carbon Monoxide Simulation Purpose – To provide an analogy of how CO mixes easier in the bloodstream than oxygen. Equipment � About ½ tsp. of ground coffee � Food colouring (blue or green is best) � Eye dropper or small straw to pipette a drop of food colouring � Small, clear bottle with lid, 2/3 full of water � CO monitor Procedure � Place the coffee grounds in the bottle of water and shake it up. The coffee colours the water very slowly. This represents oxygen in the blood. � Now add one drop of food colouring to the same bottle. Note how quickly it colours the water. This represents CO as it enters the blood. Key Points CO is picked up in the blood 240 times faster than oxygen. The oxygen level drops when carbon monoxide is present. Teaching point: can use Pico CO monitor here – showing the amount of CO in a persons lungs NC Ks3 Science Science 1.1b, 1.2a, 1.4, 2.1c, 3.3c Poisons in Tobacco Smoke Purpose -- To show the effects of tobacco smoke on a living object. Equipment � 2 small potted plants of the same variety (marigolds work well) � Aquarium with some type of lid � Smoke cabinet � 6 – 8 cigarettes � Ashtray � “What’s in tobacco smoke?” display cabinet Procedure � Place one plant and the ashtray in the aquarium, Inside the smoke cabinet. Keep the other plant out of the aquarium for control comparison. � Light a cigarette and set it on the ashtray inside the aquarium, in the smoke cabinet. Cover the aquarium to prevent the smoke from escaping. Leave the lid on the aquarium until the smoke dissipates, approximately 30 minutes. � Have the plant “smoke” twice a day for duration of three to four days. � After one week, compare the smoking plant to the non-smoking plant. Key Points � The smoking plant will look shrivelled and sickly compared to the non-smoking control plant. How Does Cigarette Smoke Affect the Growth of Plants? Nutrients Like humans and other animals, plants need sufficient nutrients to function and to grow. If the plant is deprived of these nutrients, it is unable to go through the chemical processes related to its survival and growth. Cigarettes contain more than 4,000 different chemicals, including arsenic (used as rat poison), butane (lighter fluid) and formaldehyde (fluid used in embalming). Some of these chemicals actually may benefit the plant. Carbon monoxide, for example, is able to be converted by the plant into carbon dioxide, which the plant can use readily for photosynthesis. In general, however, most chemicals released during smoking contaminate the soil and air surrounding the plant to such a degree over time that the plant eventually cannot absorb sufficient amounts of the nutrients needed to sustain itself and to grow. Microorganisms Plants are aided in growth by various microorganisms. Scientists believe these microorganisms produce enzymes and hormones that encourage plant growth. The chemicals found in cigarettes and cigarette smoke can harm or kill these microorganisms. If these microorganisms are reduced in number or are killed off altogether, the plant will not receive the enzymes and hormones that the microorganisms produce and thus won't be able to grow as well. It is much like depriving a person of a vitamin pill. Respiration and Photosynthesis Plants contain openings called stomata, which are part of the plant's respiratory system. These openings are at least partially responsible for the plant's absorption of CO2 and subsequent conversion into the glucose that the plant uses as food. Smoking can leave a residue on plants that effectively plugs up these stomata, which essentially causes the plant to "choke" and to starve. Plant growth thus is slowed because the plant cannot get what it needs for photosynthesis to take place. Molecular Change Like humans and other animals, plants cells are made up of proteins, enzymes and other components. Chemicals have an impact on the stability of these components, including the plant DNA. The chemicals can break down molecular bonds, for instance, or they can cause mutations in the genetic material. These breakdowns and changes mean that the plant cell cannot function as was originally intended, which can impact everything from water absorption to seed production. Repeated exposure to the chemicals in smoke thus may hinder plant growth and health on the molecular level. Read more: How Does Cigarette Smoke Affect the Growth of Plants? | eHow.com http://www.ehow.com/howdoes_4937678_cigarette-smoke-affect-growth-plants.html#ixzz1kSlA8R4e Teaching point: Use “What’s in tobacco smoke?” display for examples and discussion How Cigarette Smoke Affects Mould Growth on Bread Purpose To examine the effects of cigarette smoke exposure on the rate of mould growth on bread. The objective is to find out whether or not cigarette smoke effects natural growth processes such as bacteria. Equipment � Smoking chamber � Plastic jar with lid � Slices of bread � Cigarettes Procedure Place one or two slices of white bread in a clear, plastic jar with lid. Cut a hole into the jar lid and plug with an air mattress pump. Every day, twice a day, pump fresh air into the jar. The other pieces of bread are exposed to cigarette smoke inside the smoke chamber. Have the bread “smoke” twice a day. Do this consecutively for several days. After two to three days you will see mould growing at an alarming rate on the bread exposed to the cigarette smoke. This displays how unhealthy cigarette smoke is to the body and natural organisms. NC Ks3 Science Science 1.1b, 1.2a, 1.4, 2.1, 3.3c 4.Lung Capacity Purpose -- To demonstrate and discuss the effects of cigarette smoke on lung capacity. Equipment � measuring jug � 2-litre or 1-gallon jug filled with water � Basin 2/3 full of water � A bendable straw � Permanent pen � COPD lung age monitor Procedure � Fill bottle with water from measuring jug and mark levels on side of bottle with permanent pen. � Hold your thumb over the end of the 2-liter bottle and turn it upside down in the basin. Pressure will keep the water in the bottle. � Insert the short end of the bendable straw into the end of the bottle. � Ask a child to take a very deep breath and blow into the straw, emptying their lungs into the bottle. The child blows just one long breath. � Remove the straw, place your thumb over the end of the bottle, and turn it right-side up. Key Points � You've just made a spirometer - a device that doctors use to measure lung capacity. Blowing air from your lungs into the bottle displaces some of the water. � The amount of air in the bottle is the amount the child had in their lungs. The amount of air you can hold in your lungs is called lung capacity. Smoking and environmental tobacco smoke reduces lung capacity. Blowing air from your lungs into the bottle forces a measurable amount of the water out of the bottle, which adds to the volume of water in your pan. This method of measuring uses displacement – you're displacing a volume of water with a volume of air. The average total lung capacity of an adult human male is about 6 litres of air, but only a small amount of this capacity is used during normal breathing. Patients with COPD have little or no reserve capacity in their lungs Tips • Check for variations in lung capacity in people of different sizes (older students,you). Ask your class to look for a correlation between body size and lung capacity. • Have each participant test for a difference between a "normal" breath of air and an "expanded" deeper breath. � Teaching point: Use COPD lung age monitor to show varying lung ages of class, compare to spirometer readings. NC Ks3 Science Science 1.1b, 1.2a, 1.4, 2.1 c, 3.3 c 5.Smokeless Tobacco Chewing tobacco and snuff are not safe alternatives to smoking. A can of snuff has three times the nicotine as a pack of cigarettes. Smokeless tobacco causes heart disease and mouth and throat cancers. It stains the teeth and destroys the gums. Making Tobacco Juice Purpose -- To show how tobacco juice stains the teeth. Equipment � Clear glass jar with a lid � Water � Chewing tobacco Procedure � Fill the jar half full with water. � Place a wad of tobacco in the jar. � Pass the jar around the room, allowing the children to shake it. Key Points � Tobacco juice is not only full of nicotine and cancer-causing poisons but also stains the teeth and destroys the lips and gums. Tobacco Juice Stains Purpose -- To demonstrate how tobacco juice stains the teeth. Equipment: � 1 hard-boiled egg (preferably with a few cracks in it) � Enough tobacco juice to cover the egg � Toothbrush � Toothpaste � Latex gloves Procedure � Soak the egg in tobacco juice in a refrigerator for two days. � Wearing latex gloves, remove the egg and observe how stained it is. � With the toothbrush and toothpaste, brush the egg as if it were a tooth with eight or 10 strokes. Note that brushing is not able to remove all of the stain, especially in the cracks. Key Points � Over time, tobacco juice badly stains teeth. � Tobacco juice causes gums to recede and damages lips. � Tobacco use also causes bad breath. NC Ks3 Science Science 1.1b, 1.2a, 1.4, 2.1a,c, 3.3 c Sources: Some of these activities and experiments have been used extensively in classrooms throughout Trumbull County, Ohio. They have been compiled from many sources by the Trumbull County Health Department’s Health Education Department, and used in the TarWars Program Curriculum. The purpose is to demonstrate the effects of the poisons in tobacco, focusing primarily on nicotine, tar, and carbon monoxide. Tryscience – Lung capacity www.tryscience.org/teachers/files/handout_lung.pdf Wikipedia http://en.wikipedia.org/wiki/Lung_volumes Still smoking? Watch this video http://www.metacafe.com/watch/914475/still_smoking_watch_this/
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